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neutralizing antibodies to bovid herpes virus 1 (infectious bovine rhinotracheitis/infectious pustular vulvo-vaginitis) in african wildlife with special reference to the cape buffalo (syncerus caffer). 1978207748
neutralising antibodies to parainfluenza 3 virus in african wildlife, with special reference to the cape buffalo (syncerus caffer).as part of a study to assess the prevalence of common viral agents in african wildlife, nearly 3,300 sera from 44 different wild species, from eight african countries, have been examined for neutralising antibodies to parainfluenza 3 (pi3) virus. antibody was demonstrated in 20 of the 44 species examined, including seven species not previously reported as sero-positive. sera were collected between 1963 and 1977 and results indicated that infection has been widespread for a considerable time. the ...1978211274
the role of wild mammals in the epidemiology of bovine theilerioses in east africa.the theileriidae of east african wild mammals are reviewed. three species of wild bovidae were captured in east africa. they were africa cape buffalo (syncerus caffer), blue wildebeest (connochaetes taurinus) and eland (taurotragus oryx), and all were found to be naturally infected with theileria species. these animals were studied to determine the transmissibility and pathogenicity of their theilerial infections to cattle. adult rhipicephalus appendiculatus ticks, which had engorged as nymphs o ...1975803580
anthrax in wildlife in the luangwa valley, zambia.an abnormally high mortality among hippos (hippopotamus amphibius) in the luangwa river valley between june and november 1987 and estimated to number more than 4000 deaths was attributed to anthrax. several other species, particularly cape buffalo (syncerus caffer) and elephant (loxodonta africana), appear to have been affected. a smaller outbreak of anthrax in hippos occurred between august and september 1988, approximately 100 km up-river. a field study was arranged in august 1989 to assess th ...19911907048
basic haematological values in the african buffalo (syncerus caffer caffer) and in the red buffalo (syncerus caffer nanus).basic haematological values in 13 african buffaloes (syncerus caffer caffer), 11 cows and 2 bulls, and in 2 red buffaloes (syncerus caffer nanus), 1 bull and 1 cow, are reported. in the african buffalo, the erythrocyte count--mean 9.76 x 10(12) 1(-1)--ranged from 6.72 to 12.42 x 10(12) 1(-1), the haematocrit values--mean 0.391--varied from 0.32 to 0.47 and the haemoglobin content--mean 148.17--was in the range from 122.1 to 172.5 g 1(-1). for the red buffalo only individual values are given. in ...19852866870
variation among species in the endocrine control of mammary growth and function: the roles of prolactin, growth hormone, and placental lactogen.prolactin, growth hormone, and placental lactogen form a family of structurally related hormones, which may have evolved from a common ancestral peptide. prolactin and growth hormone are present in all mammals, but the biological activity associated with placental lactogen has been detected in only some groups. attempts to detect placental lactogen using bioassay and radioreceptor assay are reported and have been unsuccessful in an insectivore (the shrew), a bat, an edentate (the armadillo), a l ...19863519707
heartwater challenge of two cape buffalo (syncerus caffer).two fifth generation male cape buffalo (syncerus caffer) born in a european zoo, produced no signs of disease when injected with cowdria ruminantium infected blood. it would suggest that this species has an inate natural resistance to heartwater.19853999108
a severe spinal deformity in a cape buffalo calf (syncerus caffer) similar to "short spine lethal" of angus cattle (bos taurus). 19744856527
amino acid sequence studies on artiodactyl fibrinopeptides. i. dromedary camel, mule deer, and cape buffalo. 19676033721
anaplasma infections in wild and domestic ruminants: a review.anaplasma marginale can be transmitted, will grow and can survive in a large number of domestic and wild animals. it is pathogenic in cattle, and usually produces nonapparent or mild infections in other species. anaplasma marginale has been recovered from cattle, sheep, goats, water buffalo (bubalus bubalis), white-tailed deer (odocoileus virginianus), mule deer (odocoileus hemionus hemionus), black-tailed deer (odocoileus hemionus columbianus), pronghorn (antilocapra americana americana), rocky ...19846716555
tuberculosis in wildlife in the ruwenzori national park uganda (part i).the technique adopted for a survey of tuberculous infection in wild cape buffalo (syncerus caffer, sparrman) living in the ruwenzori national park, uganda is described and the results of gross post-mortem examinations of a random sample of 52 buffalo and a selected sample of 64 thin buffalo are tabulated. tuberculosis was found to occur in buffalo in the area of the park north of the maramagambo forest; the disease is considered to be absent from the area south of the forest. tuberculosis was fo ...19827201688
demodicosis in african buffalo (syncerus caffer caffer) in botswana.demodex cafferi causes a nodular skin disease in african buffalo (syncerus caffer caffer) resulting in eczematous lesions in severely affected animals. average prevalence in 193 animals was 28%, infection rates being highest in animals 1 to 3 years old and equal in both sexes. a granulomatous reaction occurred in the lumina of infected hair follicles with numerous mites developing in the follicles and associated sebaceous glands. the demodex in buffalo probably is not transmitted to cattle.19807463604
cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.reactivities of the monoclonal antibodies (mabs) from the workshop panel with cells from cattle, sheep, goats, cape buffalo (syncerus caffer) and waterbuck (kobus defassa) were tested. one hundred and sixty-nine mabs reacted with bovine cells and 111 with sheep cells; 86 were shown to react with goat cells, 71 with buffalo cells and 70 with waterbuck cells. some mabs cross-reacted with all five ruminants tested, and are likely to react with epitopes that are conserved in other ruminant species. ...19938310653
the trypanocidal cape buffalo serum protein is xanthine oxidase.plasma and serum from cape buffalo (syncerus caffer) kill bloodstream stages of all species of african trypanosomes in vitro. the trypanocidal serum component was isolated by sequential chromatography on hydroxylapatite, protein a-g, mono q, and superose 12. the purified trypanocidal protein had a molecular mass of 150 kda, and activity correlated with the presence of a 146-kda polypeptide detected upon reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. amino acid sequences of t ...19979284156
anti-trypanosoma brucei activity of nonprimate zoo sera.constitutive anti-trypanosoma brucei subsp. brucei s 427 clone 1 and 22 activities were evaluated in sera from 22 species of nonprimate mammals. the sera fell into 5 categories. sera from cape buffalo, giraffe, and greater kudu showed a concentration-dependent inhibition of replication of the 2 clones of organisms, which was dependent on the presence of xanthine oxidase. sera from warthog and springbok also severely limited trypanosome replication but lacked xanthine oxidase. their antitrypanoso ...199910207362
infection-associated decline of cape buffalo blood catalase augments serum trypanocidal activity.clearance of trypanosomes from the blood of infected cape buffalo was associated with the development of two responses: (i) complement-dependent and clone-specific lytic activity and (ii) complement-independent trypanocidal activity that was not restricted by trypanosome clone or species. this latter activity was mediated by h2o2 and required the presence of xanthine oxidase in serum but not the addition of purine substrates. expression of the xanthine oxidase-dependent trypanocidal activity in ...199910338483
innate and acquired resistance to african trypanosomiasis.the review discusses the current field status of human and bovine trypanosomiases, and focuses on the molecular basis of innate and acquired control of african trypanosomes in people, cattle, and cape buffalo.200111227870
purine requirements for the expression of cape buffalo serum trypanocidal activity.cape buffalo serum contains xanthine oxidase which generates trypanocidal h(2)o(2) during the catabolism of hypoxanthine and xanthine. the present studies show that xanthine oxidase-dependent trypanocidal activity in cape buffalo serum was also elicited by purine nucleotides, nucleosides, and bases even though xanthine oxidase did not catabolize those purines. the paradox was explained in part, by the presence in serum of purine nucleoside phosphorylase and adenosine deaminase, that, together wi ...200011790327
polymorphisms in mhc-dra and -drb alleles of water buffalo (bubalus bubalis) reveal different features from cattle dr alleles.seventy-five individuals of bubalus bubalis belonging to four different breeds, three of river buffalo and one of swamp buffalo, were studied for polymorphism in mhc drb (bubu-drb) and dra (bubu-dra) loci. eight alleles of bubu-drb were found, and all alleles in the swamp type were shared with the three river breeds. all alleles sampled from the breed of european origin (mediterranean) were present in breeds sampled in brazil, thus variability of this locus may have been preserved to a great ext ...200312580780
tuberculosis in the free living african (cape) buffalo (syncerus caffer caffer. sparrman). 196314081948
a panel of microsatellite and minisatellite markers for the characterisation of field isolates of theileria parva.mini- and microsatellite sequences show high levels of variation and therefore provide excellent tools for both the genotyping and population genetic analysis of parasites. herein we describe the identification of a panel of 11 polymorphic microsatellites and 49 polymorphic minisatellites of the protozoan haemoparasite theileria parva. the pcr products were run on high resolution spreadex gels on which the alleles were identified and sized. the sequences of the mini- and microsatellites were dis ...200314636680
seroprevalences of antibodies to neospora caninum and toxoplasma gondii in zoo animals.neospora caninum is an apicomplexan parasite that causes neuromuscular disease in dogs and abortions in cattle. little is known about the prevalence of antibodies to this parasite in zoo animals. sera from 556 animals, from 13 czech and slovak zoos were tested for antibodies to n. caninum and toxoplasma gondii by indirect fluorescent antibody test. antibodies to n. caninum were found in 31 of 556 zoo animals (5.6%), representing 18 of 114 species tested: eurasian wolf (canis lupus lupus), maned ...200616387445
anti-trypanosoma brucei activity in cape buffalo serum during the cryptic phase of parasitemia is mediated by antibodies.cape buffalo are reservoir hosts of african trypanosomes. they rapidly suppress population growth of the highly antigenically variable extracellular haemoprotozoa and subsequently maintain a cryptic infection. here we use in vitro cultures of trypanosomes cloned from cape buffalo blood during cryptic infection, as well as related and unrelated trypanosomes, to identify anti-trypanosome components present in cryptic-phase infection serum. trypanosome clone-specific complement-dependent trypanolyt ...200717583714
serological survey for foot-and-mouth disease virus in wildlife in eastern africa and estimation of test parameters of a nonstructural protein enzyme-linked immunosorbent assay for buffalo.in this study we estimate the seroprevalence of foot-and-mouth disease virus (fmdv) in wildlife from eastern and central africa. sera were sourced from between 1994 and 2002 from a rinderpest surveillance program. our study compared a nonstructural protein enzyme-linked immunosorbent assay (cedi test) with a virus neutralization test. the study shows that there is only a low seroprevalence of fmdv in sampled nonbuffalo species. the seroprevalence in the cape buffalo was high for sat2, lower for ...200818385460
development and evaluation of a real-time polymerase chain reaction test for the detection of theileria parva infections in cape buffalo (syncerus caffer) and cattle.corridor disease, caused by the tick-borne protozoan parasite theileria parva, is a controlled disease in south africa. the cape buffalo is the reservoir host and uninfected buffalo have become sought-after by the game industry in south africa, particularly for introduction into corridor disease-free areas. a real-time polymerase chain reaction (pcr) test for detection of t. parva dna in buffalo and cattle was developed to improve the sensitivity and specificity of the official diagnostic test p ...200818514421
climate extremes promote fatal co-infections during canine distemper epidemics in african lions.extreme climatic conditions may alter historic host-pathogen relationships and synchronize the temporal and spatial convergence of multiple infectious agents, triggering epidemics with far greater mortality than those due to single pathogens. here we present the first data to clearly illustrate how climate extremes can promote a complex interplay between epidemic and endemic pathogens that are normally tolerated in isolation, but with co-infection, result in catastrophic mortality. a 1994 canine ...200818575601
in vitro production of cattlexbuffalo hybrid embryos using cattle oocytes and african buffalo (syncerus caffer caffer) epididymal sperm.interspecies hybridization of bovids occurs between domestic cattle and at least three other species; american bison (bison bison), yak (bos grunniens) and banteng (bos banteng). birth of a cattlexbuffalo (bubalus bubalis) hybrid has reportedly occurred in russia and in china, but these reports were not authenticated. such hybrids could be important in improving livestock production and management of diseases that impede production in tropical africa. this study investigated hybridization betwee ...200919118889
can small wildlife conservancies maintain genetically stable populations of large mammals? evidence for increased genetic drift in geographically restricted populations of cape buffalo in east africa.the cape buffalo (syncerus caffer caffer) is one of the dominant and most widespread herbivores in sub-saharan africa. high levels of genetic diversity and exceptionally low levels of population differentiation have been found in the cape buffalo compared to other african savannah ungulates. patterns of genetic variation reveal large effective population sizes and indicate that cape buffalos have historically been interbreeding across considerable distances. throughout much of its range, the cap ...201020298469
theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around lake mburo national park in uganda.wildlife, especially cape buffalo (syncerus caffer), are thought to act as a reservoir for many of the important tick-borne pathogens of cattle. in this study, we have determined the prevalence of the most significant tick-borne haemoparasites in wildlife (buffalo, impala, eland and bushbuck) as well as in cattle grazing inside and neighbouring lake mburo national park (lmnp) in uganda. a high percentage of buffalo were carriers of theileria parva, theileria mutans, theileria velifera, theileria ...201120827491
diversity in the 18s ssu rrna v4 hyper-variable region of theileria spp. in cape buffalo ( syncerus caffer) and cattle from southern africa.summarysequence variation within the 18s ssu rrna v4 hyper-variable region can affect the accuracy of real-time hybridization probe-based diagnostics for the detection of theileria spp. infections. this is relevant for assays that use non-specific primers, such as the real-time hybridization assay for t. parva (sibeko et al. 2008). to assess the effect of sequence variation on this test, the theileria 18s gene from 62 buffalo and 49 cattle samples was cloned and ~1000 clones sequenced. twenty-si ...201121349232
mixed theileria infections in free-ranging buffalo herds: implications for diagnosing theileria parva infections in cape buffalo ( syncerus caffer).summarybuffalo-adapted theileria parva causes corridor disease in cattle. strict control measures therefore apply to the movement of buffalo in south africa and include mandatory testing of buffalo for the presence of t. parva. the official test is a real-time hybridization pcr assay that amplifies the v4 hypervariable region of the 18s rrna gene of t. parva, t. sp. (buffalo) and t. sp. (bougasvlei). the effect that mixed t. parva and t. sp. (buffalo)-like infections have on accurate t. parva di ...201121524322
The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle.SUMMARYCorridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infec ...201121902875
escherichia coli population structure and antibiotic resistance at a buffalo/cattle interface in southern africa.at a human/livestock/wildlife interface, escherichia coli populations were used to assess the risk of bacterial and antibiotic resistance dissemination between hosts. we used phenotypic and genotypic characterization techniques to describe the structure and the level of antibiotic resistance of e. coli commensal populations and the resistant enterobacteriaceae carriage of sympatric african buffalo (syncerus caffer caffer) and cattle populations characterized by their contact patterns in the sout ...201626712551
the african buffalo parasite theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of theileria parva antigen genes.african cape buffalo (syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus theileria, including theileria parva which causes east coast fever in cattle. a parasite, which has not yet been formally named, known as theileria sp. (buffalo) has been recognized as a potentially distinct species based on rdna sequence, since 1993. we demonstrate using reverse line blot (rlb) and sequencing of 18s rdna genes, that in an area where buffalo and cattle co- ...201526543804
molecular evolution of a central region containing b cell epitopes in the gene encoding the p67 sporozoite antigen within a field population of theileria parva.protective immunity induced by the infective sporozoite stage of theileria parva indicates a potential role for antibodies directed against conserved serologically reactive regions of the major sporozoite surface antigen p67 in vaccination to control the parasite. we have examined the allelic variation and determined the extent of b cell epitope polymorphism of the gene encoding p67 among field isolates originating from cattle exposed to infected ticks in the marula area of the rift valley in ce ...201525673078
novel snp discovery in african buffalo, syncerus caffer, using high-throughput sequencing.the african buffalo, syncerus caffer, is one of the most abundant and ecologically important species of megafauna in the savannah ecosystem. it is an important prey species, as well as a host for a vast array of nematodes, pathogens and infectious diseases, such as bovine tuberculosis and corridor disease. large-scale snp discovery in this species would greatly facilitate further research into the area of host genetics and disease susceptibility, as well as provide a wealth of sequence informati ...201223144973
rumen ciliates in the african (cape) buffalo (syncerus caffer caffer) living in the vicinity of the orpen gate entrance into kruger national park, south africa.samples of rumen contents were obtained from 10 african (cape) buffalo living in the vicinity of the orpen gate entrance into kruger national park in south africa. total number of ciliate protozoa per animal ranged from 3.15 to 23.25 x 10<sup>3</sup>. forty three different species and forms were observed, of which 35 are a new host record. the total number of species and forms per animal varied from 10 to 17. eudiplodinium maggii occurred in all 10 animals, followed by dasytricha ruminantium in ...201425112244
gene polymorphisms in african buffalo associated with susceptibility to bovine tuberculosis infection.bovine tuberculosis (btb) is a chronic, highly infectious disease that affects humans, cattle and numerous species of wildlife. in developing countries such as south africa, the existence of extensive wildlife-human-livestock interfaces poses a significant risk of mycobacterium bovis transmission between these groups, and has far-reaching ecological, economic and public health impacts. the african buffalo (syncerus caffer), acts as a maintenance host for mycobacterium bovis, and maintains and tr ...201323691232
rift valley fever in kruger national park: do buffalo play a role in the inter-epidemic circulation of virus?rift valley fever (rvf) is a zoonotic mosquito-borne virus disease of livestock and wild ruminants that has been identified as a risk for international spread. typically, the disease occurs in geographically limited outbreaks associated with high rainfall events and can cause massive losses of livestock. it is unclear how rvf virus persists during inter-epidemic periods but cryptic cycling of the virus in wildlife populations may play a role. we investigated the role that free-living african buf ...201524330522
identification and sequence characterization of novel theileria genotypes from the waterbuck (kobus defassa) in a theileria parva-endemic area in kenya.waterbuck (kobus defassa), an ungulate species endemic to the eastern african savannah, is suspected of being a wildlife reservoir for tick-transmitted parasites infective to livestock. waterbuck is infested by large numbers of rhipicephalus appendiculatus, the tick vector for theileria parva, and previous data suggests that the species may be a source of t. parva transmission to cattle. in the present study, a total of 86 cattle and 26 waterbuck blood samples were obtained from marula, a site i ...201424690249
captive breeding of the white rhinoceros, ceratotherium simum, and the cape buffalo, syncerus caffer.breeding records of 40 white rhinoceros and 155 cape buffalo were analysed. three rhinoceros cows bred in captivity, themselves conceived for the first time at 84, 87 and 95 months of age, respectively. rhinoceros cows breed throughout the year. there is no evidence of a relationship between calving interval and month of birth. calving intervals were normally distributed about the mean of 34 months and there were no significant differences between bulls, cows or sex of calf. there was no differe ...200617058447
trophy hunting and sustainability: temporal dynamics in trophy quality and harvesting patterns of wild herbivores in a tropical semi-arid savanna ecosystem.the selective nature of trophy hunting may cause changes in desirable phenotypic traits in harvested species. a decline in trophy size of preferred species may reduce hunting destination competitiveness thus compromising the sustainability of trophy hunting as a conservation tool. we explored the trophy quality and trends in harvesting patterns (i.e., 2004-2015) of cape buffalo (syncerus caffer), african elephant (loxodonta africana), greater kudu (tragelaphus strepsiceros) and sable (hippotragu ...201627736930
the genomes of three stocks comprising the most widely utilized live sporozoite theileria parva vaccine exhibit very different degrees and patterns of sequence divergence.there are no commercially available vaccines against human protozoan parasitic diseases, despite the success of vaccination-induced long-term protection against infectious diseases. east coast fever, caused by the protist theileria parva, kills one million cattle each year in sub-saharan africa, and contributes significantly to hunger and poverty in the region. a highly effective, live, multi-isolate vaccine against t. parva exists, but its component isolates have not been characterized. here we ...201526403690
an exploration of diversity among the ostertagiinae (nematoda: trichostrongyloidea) in ungulates from sub-saharan africa with a proposal for a new genus.abomasal nematodes (ostertagiine: trichostrongyloidea) representing a previously unrecognized genus and species are reported in african buffalo (syncerus caffer caffer) from kenya, uganda, and south africa. africanastrongylus buceros gen. nov. et sp. nov. is characterized by a symmetrical tapering synlophe in the cervical region and a maximum of 60 ridges in males and females. bursal structure is 2-2-1, with subequal rays 4/5, massive rays 8, and rays 9/10, and a massive dorsal lobe that is redu ...200818372646
spatial and temporal changes in group dynamics and range use enable anti-predator responses in african buffalo.the reintroduction of large predators provides a framework to investigate responses by prey species to predators. considerable research has been directed at the impact that reintroduced wolves (canis lupus) have on cervids, and to a lesser degree, bovids, in northern temperate regions. generally, these impacts alter feeding, activity, and ranging behavior, or combinations of these. however, there are few studies on the response of african bovids to reintroduced predators, and thus, there is limi ...201222834371
genetic structure of fragmented southern populations of african cape buffalo (syncerus caffer caffer).african wildlife experienced a reduction in population size and geographical distribution over the last millennium, particularly since the 19th century as a result of human demographic expansion, wildlife overexploitation, habitat degradation and cattle-borne diseases. in many areas, ungulate populations are now largely confined within a network of loosely connected protected areas. these metapopulations face gene flow restriction and run the risk of genetic diversity erosion. in this context, w ...201425367154
the prion protein gene polymorphisms associated with bovine spongiform encephalopathy susceptibility differ significantly between cattle and buffalo.prion protein, encoded by the prion protein gene (prnp), plays a crucial role in the pathogenesis of transmissible spongiform encephalopathies (tses). several polymorphisms within the prnp are known to be associated with influencing bovine spongiform encephalopathy (bse) susceptibility in cattle, namely two insertion/deletion (indel) polymorphisms (a 23-bp indel in the putative promoter and a 12-bp indel in intron 1), the number of octapeptide repeats (octarepeats) present in coding sequence (cd ...201526319996
transcriptional profiling of inflammatory cytokine genes in african buffaloes (syncerus caffer) infected with theileria parva.theileria parva (t. parva) causes east coast fever (ecf), which is of huge economic importance to eastern and southern african countries. in a previous bovine model, inflammatory cytokines were closely associated with disease progression in animals experimentally infected with t. parva. the african cape buffalo (syncerus caffer), the natural reservoir for t. parva, is completely resistant to ecf despite a persistently high parasitaemia following infection with t. parva. characterizing basic immu ...201222854202
geographic distribution of theileria sp. (buffalo) and theileria sp. (bougasvlei) in cape buffalo (syncerus caffer) in southern africa: implications for speciation.strict control measures apply to movement of buffalo in south africa including testing for theileria parva, the causative agent of corridor disease in cattle. the official test is a real-time hybridization pcr assay that amplifies the 18s rrna v4 hyper-variable region of t. parva, t. sp. (buffalo) and t. sp. (bougasvlei). mixed infections with the latter organisms affect diagnostic sensitivity due to pcr suppression. while the incidence of mixed infections in the corridor disease endemic region ...201424229841
disease at the wildlife-livestock interface: acaricide use on domestic cattle does not prevent transmission of a tick-borne pathogen with multiple hosts.several prominent and economically important diseases of livestock in east africa are caused by multi-host pathogens that also infect wildlife species, but management strategies are generally livestock focused and models of these diseases tend to ignore the role of wildlife. we investigate the dynamics of a multi-host tick-borne disease in order to assess the efficacy of tick control from an ecological perspective. we examined the efficacy of a widespread measure of tick control and developed a ...201424315187
investigating the diversity of the 18s ssu rrna hyper-variable region of theileria in cattle and cape buffalo (syncerus caffer) from southern africa using a next generation sequencing approach.molecular classification and systematics of the theileria is based on the analysis of the 18s rrna gene. reverse line blot or conventional sequencing approaches have disadvantages in the study of 18s rrna diversity and a next-generation 454 sequencing approach was investigated. the 18s rrna gene was amplified using rlb primers coupled to 96 unique sequence identifiers (mids). theileria positive samples from african buffalo (672) and cattle (480) from southern africa were combined in batches of 9 ...201627084674
detection of antibodies to tuberculosis antigens in free-ranging lions (panthera leo) infected with mycobacterium bovis in kruger national park, south africa.bovine tuberculosis (tb), caused by mycobacterium bovis, has become established in kruger national park, south africa, in the cape buffalo (syncerus caffer) population and in other species. tb in prey species has resulted in infection and morbidity in the resident lion (panthera leo) prides. the only validated live animal test currently available for lions is the intradermal tuberculin test. because this test requires capture twice, 72 hr apart, of free-ranging lions to read results, it is logis ...201222779235
haemoparasite prevalence and theileria parva strain diversity in cape buffalo (syncerus caffer) in uganda.cape buffalo (syncerus caffer) are considered to be an important reservoir for various tick-borne haemoparasites of veterinary importance. in this study we have compared the haemoparasite carrier prevalence in buffalo from four geographically isolated national parks in uganda [lake mburo national park (lmnp), queen elizabeth national park (qenp), murchison falls national park (mfnp) and kidepo valley national park (kvnp)]. differences were seen in haemoparasite prevalence in buffalo from the fou ...201021074945
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