| helicobacter bilis sp. nov., a novel helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. | a fusiform bacterium with 3 to 14 multiple bipolar sheathed flagella and periplasmic fibers wrapped around the cell was isolated from the liver, bile, and lower intestine of aged, inbred mice. the bacteria grew at 37 and 42 degrees c under microaerophilic conditions, rapidly hydrolyzed urea, were catalase and oxidase positive, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and were resistant to both cephalothin and nalidixic acid but sensitive to metronidazole. on th ... | 1995 | 7536217 |
| identification of murine helicobacters by pcr and restriction enzyme analyses. | three murine helicobacter species have recently been identified: helicobacter hepaticus, helicobacter muridarum, and helicobacter bilis. infections with h. hepaticus and h. bilis have been associated with hepatitis and hepatic neoplasia. in this study, oligonucleotide primers were designed from regions of the 16s rrna gene that are conserved among members of the helicobacter genus. the assay amplified the expected 374-bp product from all three rodent helicobacter species and was able to detect a ... | 1996 | 8815113 |
| helicobacter canis isolated from a dog liver with multifocal necrotizing hepatitis. | on the basis of biochemical, phenotypic, and 16s rrna analysis, a novel gram-negative bacterium, isolated from normal and diarrheic dogs as well as humans with gastroenteritis, has been recently named helicobacter canis. a 2-month-old female crossbred puppy was submitted to necropsy with a history of weakness and vomiting for several hours prior to death. the liver had multiple and slightly irregular yellowish foci up to 1.5 cm in diameter. histologically, the liver parenchyma contained randomly ... | 1996 | 8880504 |
| serodiagnosis of helicobacter hepaticus infection in mice by an enzyme-linked immunosorbent assay. | helicobacter hepaticus is a newly recognized bacterium associated with chronic active hepatitis, hepatic carcinoma, and inflammatory bowel disease in mice. currently, fecal or tissue pcr, fecal culture, or histologic examination of silver-stained liver sections is used to diagnose h. hepaticus infection. in this report, we describe an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of h. hepaticus infection in mice with a membrane digest preparation of h. hepaticus as the antigen. se ... | 1997 | 9114413 |
| helicobacter rodentium sp. nov., a urease-negative helicobacter species isolated from laboratory mice. | a spiral-shaped bacterium with bipolar, single, nonsheathed flagella was isolated from the intestines of laboratory mice. the organism grew at 37 and 42 degrees c under microaerobic and anaerobic conditions, did not hydrolyze urea, was weakly positive for catalase and oxidase, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and was resistant to cephalothin and nalidixic acid. this is the first urease-negative, murine helicobacter spp. isolated from intestines. also, h ... | 1997 | 9226892 |
| helicobacter bilis-induced inflammatory bowel disease in scid mice with defined flora. | helicobacter bilis has been isolated from aged inbred mice with multifocal chronic hepatitis and from scid mice with diarrhea, proliferative typhlitis, and colitis. to determine the pathogenic potential of h. bilis, we inoculated 4-week-old female tac:icr:ha(icr)-scidfdf mice by intraperitoneal injection of approximately 10(8) cfu of h. bilis in phosphate-buffered saline (pbs) (n = 15) or pbs alone (n = 10) and necropsied them at 7 weeks postinfection. sham-inoculated mice had no significant gro ... | 1997 | 9353076 |
| inflammatory large bowel disease in immunodeficient rats naturally and experimentally infected with helicobacter bilis. | proliferative and ulcerative typhlitis, colitis, and proctitis were found incidentally in a breeding colony of male athymic nude (cr:nih-rnu) rats. within the crypts of the large intestine, modified steiner's silver stain revealed spiral organisms that were identified by culture, polymerase chain reaction, and sequencing to be helicobacter bilis. the large bowel disease was reproduced in h. bilis-free male athymic nude rats that were injected intraperitoneally with a culture of h. bilis from the ... | 1998 | 9598583 |
| enterohepatic lesions in scid mice infected with helicobacter bilis. | helicobacter bilis is a recently identified species that colonizes the intestine and liver of mice. in immunocompetent mice, infections have been associated with mild hepatitis, and in immunocompromised mice, inflammatory bowel disease has been induced by intraperitoneal inoculation of the organism. we report inoculation of 6-week-old c.b-17 scid/scid mice by gastric gavage with approximately 10(7) h. bilis colony-forming units. groups of mice were euthanized and necropsied 12, 24, and 36 weeks ... | 1998 | 10090038 |
| helicobacter bilis/helicobacter rodentium co-infection associated with diarrhea in a colony of scid mice. | an outbreak of diarrhea spanning 3 months occurred in a breeding colony of scid/trp53 knockout mice. approximately a third of the 150 mice were clinically affected, with signs ranging from mucoid or watery diarrhea to severe hemorrhagic diarrhea with mortality. helicobacter bilis and the newly recognized urease-negative organism h. rodentium were isolated from microaerobic culture of feces or cecal specimens from affected mice. dual infection with h. bilis and h. rodentium were confirmed by cult ... | 1998 | 10090057 |
| essential role of helicobacter pylori gamma-glutamyltranspeptidase for the colonization of the gastric mucosa of mice. | constitutive expression of gamma-glutamyltranspeptidase (ggt) activity is common to all helicobacter pylori strains, and is used as a marker for identifying h. pylori isolates. helicobacter pylori ggt was purified from sonicated extracts of h. pylori strain 85p by anion exchange chromatography. the n-terminal amino acid sequences of two of the generated endo-proteolysed peptides were determined, allowing the cloning and sequencing of the corresponding gene from a genomic h. pylori library. the h ... | 1999 | 10200957 |
| cloning and expression of an immunogenic membrane-associated protein of helicobacter hepaticus for use in an enzyme-linked immunosorbent assay. | helicobacter hepaticus is a bacterial pathogen that causes chronic active hepatitis and inflammatory bowel disease in mice. the purpose of this study was to develop a recombinant antigen-based enzyme-linked immunosorbent assay (elisa) to detect h. hepaticus-infected mice. a genomic library of h. hepaticus was constructed and was screened with sera from h. hepaticus-infected mice. a 459-bp open reading frame that coded for an 18-kda immunoreactive protein, map18, was identified. the gene had high ... | 1999 | 10473529 |
| comparison of iron uptake in different helicobacter species. | comparison of iron uptake of four helicobacter species (helicobacter pylori, helicobacter felis, helicobacter acinonyx, and helicobacter mustelae), associated with various degrees of gastritis in their respective host, with five other species which colonize the intestinal tract of various animals (helicobacter fennelliae, helicobacter cinaedi, helicobacter muridarum, helicobacter bilis, and helicobacter hepaticus), demonstrated that the iron acquisition system differed according to the ecologica ... | 1999 | 10540911 |
| phylogenetic characterization of 'candidatus helicobacter bovis', a new gastric helicobacter in cattle. | recently helicobacter-like organisms have been reported in the pyloric part of the abomasum of calves and adult cattle. cultivation of these spiral bacteria has not been successful to date. in the present study, comparative 16s rdna sequence analysis was used to determine the taxonomic position of these bacteria. seven abomasal biopsies of adult cattle were sampled from different belgian and dutch farms. in all samples the presence of helicobacter-like organisms was demonstrated by biochemical, ... | 1999 | 10555352 |
| identification of helicobacter pylori and other helicobacter species by pcr, hybridization, and partial dna sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. | helicobacter pylori was identified in human liver tissue by pcr, hybridization, and partial dna sequencing. liver biopsies were obtained from patients with primary sclerosing cholangitis (n = 12), primary biliary cirrhosis (n = 12), and noncholestatic liver cirrhosis (n = 13) and (as controls) normal livers (n = 10). pcr analyses were carried out using primers for the helicobacter genus, helicobacter pylori (the gene encoding a species-specific 26-kda protein and the 16s rrna), helicobacter bili ... | 2000 | 10698999 |
| characteristics of helicobacter pylori natural transformation. | for helicobacter pylori, which exhibits substantial genetic diversity, many strains are naturally competent for transformation by exogenous dna. to better understand the mechanism of natural transformation and its role in the generation of diversity, we sought to systematically identify factors important for natural transformation in h. pylori. we now show that the highest frequency of h. pylori transformation occurs when dna is introduced prior to exponential phase growth, and that it is a satu ... | 2000 | 10802184 |
| slaughter pigs are commonly infected by closely related but distinct gastric ulcerative lesion-inducing gastrospirilla. | an association between (unculturable) gastrospirillum-like organisms (glo) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. in dogs glo detected by microscopy may represent several helicobacter species or subspecies. therefore we investigated which helicobacter spp. are present in stomachs of swine and their possible association with ulcerative lesions of the pars oesophagea. the presence of helicobacter spp. in the antrum and pars oesophagea in 122 stomachs o ... | 2000 | 10878060 |
| identification of a unique helicobacter species by 16s rrna gene analysis in an abdominal abscess from a patient with x-linked hypogammaglobulinemia. | a unique helicobacter species, mz640285, was isolated from a patient with x-linked hypogammaglobulinemia suffering from recurrent abdominal abscesses and was identified by 16s rrna gene sequence analysis. in the phylogenetic tree, the isolate fell into a cluster which included flexispira rappini, helicobacter bilis, and helicobacter sp. strain mainz. helicobacters are being increasingly recognized as pathogens in immunocompromised hosts. these fastidious bacteria are not easily cultured in the r ... | 2000 | 10878075 |
| 'flexispira rappini' strains represent at least 10 helicobacter taxa. | 'flexispira rappini' is a provisional name given to gram-negative, microaerophilic, motile, spindle-shaped micro-organisms with spiral periplasmic fibres and bipolar tufts of sheathed flagella. several investigators, including kirkbride, romero, and archer isolated strains possessing this morphology. previously, the phylogenetic position of three 'flexispira rappini' strains was determined by 165 rrna sequencing, which indicated that flexispira were members of the genus helicobacter. as more org ... | 2000 | 11034487 |
| helicobacters of possible zoonotic origin: a review. | since the isolation of helicobacter pylori, many new helicobacter species have been identified from the gastrointestinal tract in humans and animals. in humans, a spiral organism different from h. pylori and provisionally named "helicobacter heilmannii", has been associated with gastritis, gastric ulceration and to a lesser degree, gastric cancer. in addition helicobacter cinaedi, helicobacter fennelliae, helicobacter pullorum and "flexispira rappini" have been isolated from cases of enteric dis ... | 2000 | 11233522 |
| characterization of proteins in the outer membrane preparation of a murine pathogen, helicobacter bilis. | helicobacter bilis is a bacterial pathogen associated with multifocal hepatitis and inflammatory bowel disease in certain strains of mice. this bacterium colonizes the liver, bile, and lower intestine in mice and has also been isolated from a wide spectrum of laboratory animals. in this study, proteins present in the outer membrane preparation (omp) of four h. bilis strains isolated from a mouse, a dog, a rat, and a gerbil were characterized and compared with that of helicobacter pylori, a human ... | 2001 | 11292784 |
| coinfection of enteric helicobacter spp. and campylobacter spp. in cats. | during a 6-year period, 64 of 227 commercially reared cats had microaerobic bacteria isolated from their feces. all the isolates were initially identified as campylobacter-like organisms based on biochemical and phenotypic characteristics. dna extractions from 51 of these isolates were subjected to pcr using primers specific for helicobacter spp. and campylobacter spp. of the isolates, 92% (47 of 51 isolates) were positive for campylobacter spp., 41% (21 of 51 isolates) were positive for helicob ... | 2001 | 11376052 |
| cholangiohepatitis and inflammatory bowel disease induced by a novel urease-negative helicobacter species in a/j and tac:icr:hascidfrf mice. | helicobacter bilis and h. hepaticus, both urease-positive intestinal helicobacters of mice, have been shown experimentally to induce proliferative typhlocolitis in scid mice. we recently isolated a urease-negative helicobacter sp. (h. sp.) that also induced proliferative typhlocolitis in pilot studies in scid mice. to determine the pathogenic potential of h. sp. in immunocompromised and immunocompetent mice, 5-week old male a/j or tac:icr:ha(icr)-scidfrf mice were inoculated by intraperitoneal ( ... | 2001 | 11393169 |
| helicobacter-induced inflammatory bowel disease in il-10- and t cell-deficient mice. | inflammatory bowel disease (ibd) is thought to result from a dysregulated mucosal immune response to luminal microbial antigens, with t lymphocytes mediating the colonic pathology. infection with helicobacter spp has been reported to cause ibd in immunodeficient mice, some of which lack t lymphocytes. to further understand the role of t cells and microbial antigens in triggering ibd, we infected interleukin (il)-10(-/-), recombinase-activating gene (rag)1(-/-), t-cell receptor (tcr)-alpha(-/-), ... | 2001 | 11518689 |
| helicobacter bilis infection accelerates and h. hepaticus infection delays the development of colitis in multiple drug resistance-deficient (mdr1a-/-) mice. | mdr1a-deficient mice lack p-glycoprotein and spontaneously develop colitis with age. helicobacter spp. are gram-negative organisms that have been associated with colitis in certain mouse strains, but helicobacter spp. have been excluded as contributing to the spontaneous colitis that develops in mdr1a-/- mice. we wished to determine whether infection with either h. bilis or h. hepaticus would accelerate the development of inflammatory bowel disease (ibd) in mdr1a-/- mice. we found that h. bilis ... | 2002 | 11839595 |
| influence of activated charcoal, porcine gastric mucin and beta-cyclodextrin on the morphology and growth of intestinal and gastric helicobacter spp. | bile-tolerant helicobacter spp. are emerging human and animal pathogens. however, due to their fastidious nature, which requires nutrient-rich complex media to grow, infection with these bacteria may be underestimated. the accumulation of toxic metabolites in cultures may be one of the main obstacles for successful culture of these organisms. the present study examined various potential growth-enhancing substances for helicobacter spp. and, furthermore, how they may affect spiral to coccoid conv ... | 2002 | 11882701 |
| evaluation of diagnostic methods for helicobacter bilis infection in laboratory mice. | disease-susceptible (c3h) and -resistant (b6) immunocompetent and immunodeficient (c3h-scid and b6-rag1) mice were examined up to 10 weeks after inoculation with helicobacter bilis (a prototype species of proven virulence). infection was monitored weekly by use of fecal culture, polymerase chain reaction (pcr) nucleic acid amplification, membrane extract enzyme-linked immunosorbent assay (elisa), and histologic examination. all mice became infected by three to five weeks after inoculation, on th ... | 2001 | 11924799 |
| cloning and expression of a helicobacter bilis immunoreactive protein. | in an effort to identify immunoreactive helicobacter bilis antigens with potential for serodiagnosis, sera from mice experimentally infected with h. bilis were used to screen an h. bilis genomic dna expression library. among 17 immunoreactive clones, several contained sequences that encoded a predicted 167-kda protein (p167). five overlapping p167 peptides (p167a to p167e) of approximately 40 kda each were generated and tested. immune sera reacted with fragments p167c and p167d at dilutions of 1 ... | 2002 | 11986271 |
| immunogenic proteins of helicobacter pullorum, helicobacter bilis and helicobacter hepaticus identified by two-dimensional gel electrophoresis and immunoblotting. | the ecological niches occupied by various species of helicobacter are not yet known and the full spectrum of diseases associated with helicobacter infections are not yet defined. since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. immunogenic cell surface proteins of helicobacter pullorum, helicobacter bili ... | 2002 | 12112861 |
| association between helicobacter bilis in bile and biliary tract malignancies: h. bilis in bile from japanese and thai patients with benign and malignant diseases in the biliary tract. | japan and thailand have high incidences of bile duct carcinoma and gallstones. the presence of helicobacter bilis (h. bilis) detected by polymerase chain reaction (pcr) and 16s rrna analysis in bile samples from chileans with chronic cholecystitis was reported. the association between h. bilis in bile and biliary tract malignancies has not been investigated, and therefore the aim of this study is to determine whether malignant diseases of the biliary tract are associated with the presence of h. ... | 2002 | 12149151 |
| immune responses to bile-tolerant helicobacter species in patients with chronic liver diseases, a randomized population group, and healthy blood donors. | bile-tolerant helicobacter species such as helicobacter pullorum, helicobacter bilis, and helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (cld) in humans. antibody responses to cell surface proteins of h. pullorum, h. bilis, and h. hepaticus in serum samples from patients with cld, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (elisa). the ... | 2002 | 12414744 |
| cytolethal distending toxin b gene (cdtb) homologues in taxa 2, 3 and 8 and in six canine isolates of helicobacter sp. flexispira. | the presence of the cytolethal distending toxin b gene (cdtb) was examined in eight helicobacter sp. flexispira reference strains, helicobacter trogontum atcc 700114(t) and 12 finnish porcine h. trogontum strains and canine flexispira isolates. part of the cdtb gene was amplified by pcr with degenerate primers vat2 and dhf1, cloned and sequenced. the presence/absence of the cdtb gene as determined by pcr was confirmed by southern hybridization and toxin production by hela cell-line experiments. ... | 2003 | 12543914 |
| the rapid detection of low molecular mass proteins differentially expressed under biological stress for four helicobacter spp. using proteinchip technology. | helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. in addition h. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from h. ... | 2003 | 12627380 |
| glycoconjugate binding of gastric and enterohepatic helicobacter spp. | helicobacter pylori is able to utilize several lectin-like, protein-carbohydrate interactions for binding to mucins, cell surfaces, and extracellular matrix proteins. as determined by hemagglutination assays and binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms, strains of gastric helicobacters and enterohepatic helicobacters, including helicobacter canis, helicobacter hepaticus, and helicobacter bilis, also demonstrated evidence for the presence of lectin-hemagg ... | 2003 | 12704182 |
| helicobacter sp. flexispira 16s rdna taxa 1, 4 and 5 and finnish porcine helicobacter isolates are members of the species helicobacter trogontum (taxon 6). | the term 'flexispira' refers to micro-organisms with a particular morphology: fusiform-shaped with helical periplasmic fibrils and bipolar tufts of sheathed flagella. two flexispira taxa have been formally named, helicobacter bilis and helicobacter trogontum, a third named species is helicobacter aurati and eight additional 16s rrna sequence-based flexispira taxa have been described by dewhirst et al. (int j syst evol microbiol 50, 1781-1787, 2000) and given the provisional designation helicobac ... | 2003 | 12710608 |
| potential for proteomic profiling of helicobacter pylori and other helicobacter spp. using a proteinchip array. | the helicobacter genus is associated with a wide spectrum of pathologies in the gastrointestinal tract. however, in contrast to helicobacter pylori, few data are available regarding proteomic characteristics of enterohepatic helicobacters. proteomic analysis of this genus has predominantly utilised two-dimensional gel electrophoresis methodology. in the present study we applied an innovative technique using proteinchip arrays coupled with surface-enhanced laser desorption/ionisation time of flig ... | 2003 | 12738385 |
| common presence of helicobacter dna in the gallbladder of patients with gallstone diseases and controls. | several species of helicobacter colonise the biliary tract of animals and cause hepatobiliary diseases. helicobacter species have also been identified in the gallbladder of a high proportion of chilean patients with gallbladder cancer. | 2003 | 12801034 |
| assessment of pcr-dgge for the identification of diverse helicobacter species, and application to faecal samples from zoo animals to determine helicobacter prevalence. | helicobacter species are fastidious bacterial pathogens that are difficult to culture by standard methods. a pcr-denaturing gradient gel electrophoresis (pcr-dgge) technique for detection and identification of different helicobacter species was developed and evaluated. the method involves pcr detection of helicobacter dna by genus-specific primers that target 16s rdna and subsequent differentiation of helicobacter pcr products by use of dgge. strains are identified by comparing mobilities of unk ... | 2003 | 12909652 |
| increased prevalence of seropositivity for non-gastric helicobacter species in patients with autoimmune liver disease. | various helicobacter species have been isolated from the stomach, intestinal tract and liver of a variety of mammalian and some avian species, and helicobacter dna has been detected in human bile and liver samples. an immunoblot assay was established to analyse serum antibody responses to non-gastric helicobacter species in patients with autoimmune liver diseases, in comparison with healthy individuals. sera from 36 patients with primary sclerosing cholangitis (psc), 21 with primary biliary cirr ... | 2003 | 14532338 |
| assessing the risk of transmission of three infectious agents among mice housed in a negatively pressurized caging system. | previous studies from our institution have shown that ventilated caging run at negative pressure to a mouse room dramatically reduced exposure of personnel to the major mouse allergen, mus m 1. the current study was designed to determine whether negative cage ventilation posed an inordinate risk for spread of infectious agents between cages and/or racks. b6;129s-tnfsf5(tm1imx)/j (tnf) mice, which were naturally and persistently infected with pneumocystis carinii, helicobacter bilis, and pasteure ... | 2003 | 14615955 |
| use of the p167 recombinant antigen for serodiagnosis of helicobacter bilis. | helicobacter bilis is widespread among research mouse colonies. serodiagnosis of helicobacter infections involves use of bacterial lysates or membrane antigen preparations that lack specificity, necessitating the need to identify a specific and sensitive antigen. a previously reported recombinant protein (p167) was evaluated for use as an h. bilis-specific antigen for serologic testing. seventy-six mice naturally infected with helicobacter spp. were identified from commercially bred or sentinel ... | 2004 | 15027617 |
| regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in mdr2 (abcb4) knockout mice. | because the mechanisms leading to bile duct damage in sclerosing cholangitis are unknown, we aimed to determine the pathogenesis of bile duct injury in multidrug resistance gene (mdr2) (abcb4) knockout mice (mdr2(-/-)) as a novel model of the disease. | 2004 | 15236191 |
| comparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters. | the lipopolysaccharide of helicobacter pylori plays an important role in colonization and pathogenicity. the present study sought to compare structural and biological features of lipopolysaccharides from gastric and enterohepatic helicobacter spp. not previously characterized. | 2004 | 15270745 |
| helicobacter bilis infection in biliary tract cancer. | biliary tract cancer is a highly fatal disease with poor prognosis, but the aetiology is poorly understood. | 2004 | 15298612 |
| recombinant helicobacter bilis protein p167 for mouse serodiagnosis in a multiplex microbead assay. | infection of mice with helicobacter bilis is widespread in research and commercial mouse colonies. therefore, sensitive, specific, and high-throughput assays are needed for rapid and accurate testing of mice in large numbers. this report describes a novel multiplex assay, based on fluorescent microbeads, for serodetection of h. bilis infection. the assay requires only a few microliters of serum to perform and is amenable to a high-throughput format. individual microbead sets were conjugated to p ... | 2004 | 15539512 |
| pathogenicity of helicobacter rodentium in a/jcr and scid mice. | helicobacter rodentium was first recognized as a potential pathogen when it was isolated, along with helicobacter bilis, from a colony of scid/trp53 knockout mice with diarrhea. clinical disease in these mice was more severe than that previously reported in mice infected with h. bilis alone, thus suggesting that h. rodentium contributed to the pathogenesis of enteritis. the purpose of the study reported here was to address two questions: is h. rodentium pathogenic in mice, and when co-infection ... | 2004 | 15575369 |
| helicobacter bilis-associated hepatitis in outbred mice. | although helicobacter bilis infects mice worldwide, it is not known whether h. bilis causes enterohepatic disease in outbred swiss webster (sw) mice. intestinal and liver specimens from four groups of 39 sw mice, five of which were treated with creatine in the drinking water, were obtained for culture for the presence of h. bilis and were analyzed as to whether infection status was associated with h. bilis seroconversion and/or hepatitis. helicobacter bilis was isolated from the colon of all 27 ... | 2004 | 15575372 |
| a novel enterohepatic helicobacter species 'helicobacter mastomyrinus' isolated from the liver and intestine of rodents. | a number of novel helicobacter species have been isolated from both animals and humans. many of these helicobacters colonize the lower gastrointestinal tract and hepatobiliary tract and are associated with diseases. | 2005 | 15691316 |
| extension of the species helicobacter bilis to include the reference strains of helicobacter sp. flexispira taxa 2, 3 and 8 and finnish canine and feline flexispira strains. | the evolution and taxonomy of enterohepatic helicobacter species with flexispira morphology were studied by a polyphasic approach including phenotypic characterization, analysis of 16s rrna and ureb gene sequences and dot-blot dna-dna hybridization of whole genomic dna. in addition, available phylogenetic data on the hsp60 gene were used in the analysis. the study included 14 finnish canine and feline flexispira strains, the reference strains of helicobacter sp. flexispira taxa 2, 3 and 8 and he ... | 2005 | 15774681 |
| identification of cholelithogenic enterohepatic helicobacter species and their role in murine cholesterol gallstone formation. | helicobacter spp are common inhabitants of the hepatobiliary and gastrointestinal tracts of humans and animals and cause a variety of well-described diseases. recent epidemiologic results suggest a possible association between enterohepatic helicobacter spp and cholesterol cholelithiasis, chronic cholecystitis, and gallbladder cancer. to test this, we prospectively investigated the effects of helicobacter spp infection in cholesterol gallstone pathogenesis in the highly susceptible c57l/j mouse ... | 2005 | 15825083 |
| significance of transiently positive enzyme-linked immunosorbent assay results in detection of helicobacter pylori in stool samples from children. | in young children, the significance of stool samples transiently positive for helicobacter pylori antigen is unknown. as part of a larger prospective study on enteric infections, stool samples were obtained from 323 children at two time points 3 months apart and tested for h. pylori antigen using a commercially available enzyme-linked immunosorbent assay (elisa) test. seminested pcr for a helicobacter-specific 16s rrna gene was performed on all 26 pairs reverting from positive to negative (trans ... | 2005 | 15872245 |
| dual infection with helicobacter bilis and helicobacter hepaticus in p-glycoprotein-deficient mdr1a-/- mice results in colitis that progresses to dysplasia. | patients with inflammatory bowel disease (ibd) are at increased risk for developing high-grade dysplasia and colorectal cancer. animal ibd models that develop dysplasia and neoplasia may help elucidate the link between inflammation and colorectal cancer. mdr1a-/- mice lack the membrane efflux pump p-glycoprotein and spontaneously develop ibd that can be modulated by infection with helicobacter sp: h. bilis accelerates development of colitis while h. hepaticus delays disease. in this study, we de ... | 2005 | 15920164 |
| monitoring a mouse colony for helicobacter bilis using a helicobacter-genus-specific nested pcr. | although helicobacter infections of laboratory mice are usually subclinical, they may interfere with in vivo experiments and thus may lead to misinterpretation of data. as such, it is important to provide a means to unequivocally identify infections with murine helicobacter spp. in the present study, a nested polymerase chain reaction (pcr) was established and shown to be 10 to 100 times more sensitive than the single-step pcr commonly used for routine diagnosis of helicobacter spp. experimental ... | 2005 | 16197707 |
| gallbladder cancer worldwide: geographical distribution and risk factors. | gallbladder cancer is a relatively rare neoplasm that shows, however, high incidence rates in certain world populations. the interplay of genetic susceptibility, lifestyle factors and infections in gallbladder carcinogenesis is still poorly understood. age-adjusted rates were calculated by cancer registry-based data. epidemiological studies on gallbladder cancer were selected through searches of literature, and relative risks were abstracted for major risk factors. the highest gallbladder cancer ... | 2006 | 16397865 |
| serum antibodies to enterohepatic helicobacter spp. in patients with chronic liver diseases and in a population with high prevalence of h. pylori infection. | enteric helicobacter species might be a risk factor for chronic liver and biliary tract diseases. | 2006 | 16464652 |
| induction of differential immune reactivity to members of the flora of gnotobiotic mice following colonization with helicobacter bilis or brachyspira hyodysenteriae. | aberrant host immune responses to bacterial components of the resident microflora may initiate and perpetuate gastrointestinal inflammation. to investigate how microbial perturbation promotes host immunological responsiveness to commensal bacteria and contributes to the development of typhlocolitis, we selectively colonized defined (altered schaedler) flora c3h mice with either helicobacter bilis or brachyspira hyodysenteriae. following selective colonization, tissues were analyzed for gross/his ... | 2006 | 16698302 |
| extragastric manifestations of helicobacter pylori infection--other helicobacter species. | recent studies have indicated a strong link between helicobacter pylori and idiopathic thrombocytopenic purpura and iron deficiency anemia. interesting results have also been obtained for ischemic heart disease, though most putative associations between h. pylori infection and extragastric disease remain speculative. with regard to other helicobacter species, helicobacter felis has been shown to play a role in gastric carcinogenesis in mouse models. an increased susceptibility to cholesterol gal ... | 2006 | 16925612 |
| association of helicobacter with cholangiohepatitis in cats. | infection with helicobacter spp. is increasingly linked with hepatobiliary inflammation and neoplasia in people and in a variety of animals. we sought to determine if helicobacter species infection is associated with cholangiohepatitis in cats. deoxyribonucleic acid was extracted from tissue blocks from cats with cholangiohepatitis (32), noninflammatory liver disease (13), and cats with normal liver histology (4). deoxyribonucleic acid was polymerase chain reaction-amplified with 2 sets of helic ... | 2006 | 16955803 |
| helicobacter bilis triggers persistent immune reactivity to antigens derived from the commensal bacteria in gnotobiotic c3h/hen mice. | infection with helicobacter species has been associated with the development of mucosal inflammation and inflammatory bowel disease (ibd) in several mouse models. however, consensus regarding the role of helicobacter as a model organism to study microbial-induced ibd is confounded by the presence of a complex colonic microbiota. | 2007 | 17145736 |
| specific and quantitative detection of pcr products from clostridium piliforme, helicobacter bilis, h. hepaticus, and mouse hepatitis virus infected mouse samples using a newly developed electrochemical dna chip. | we developed a microfabricated electrochemical dna chip for detection of polymerase chain reaction (pcr) products from 16s rrna sequences of clostridium piliforme (cp), helicobacter bilis (hb) and helicobacter hepaticus (hh), and the nucleocapsid protein gene of mouse hepatitis virus (mhv). this chip does not require dna labeling, and the hybridization signal can be detected as an anodic current. the average anodic currents of 9 (cp), 5 (hb), 8 (hh) and 7 (mhv) pcr positive samples derived from ... | 2007 | 17267057 |
| eradication of helicobacter bilis and h. hepaticus from infected mice by using a medicated diet. | infection of laboratory mice with helicobacter spp. is a serious problem for many laboratory animal facilities worldwide. rederivation and antibiotic treatment are two of the most common methods used to eliminate the bacterial infection from rodent colonies. forty-seven newly imported mice were suspected to be positive for helicobacter infection based on pcr analysis of pooled fecal samples from sentinel animals. we treated the mice with a medicated feed containing four antibiotic compounds (amo ... | 2007 | 17450169 |
| helicobacter bilis: bacterial provocateur orchestrates host immune responses to commensal flora in a model of inflammatory bowel disease. | | 2007 | 17566023 |
| serum biomarkers in a mouse model of bacterial-induced inflammatory bowel disease. | the diagnosis and classification of inflammatory bowel disease (ibd) require both clinical and histopathologic data. serum biomarkers would be of considerable benefit to noninvasively monitor the progression of disease, assess effectiveness of therapies, and assist in understanding disease pathogenesis. currently, there are limited noninvasive biomarkers for monitoring disease progression in animal ibd models, which are used extensively to develop new therapies and to understand ibd pathogenesis ... | 2008 | 18095317 |
| cross-reactivity between immune responses to helicobacter bilis and helicobacter pylori in a population in thailand at high risk of developing cholangiocarcinoma. | helicobacter bilis dna has been detected in human tissue and is a candidate for etiologic investigations on the causes of hepatic and biliary tract diseases, but reliable serologic tests need to be developed in order to pursue such investigations. the scope of this study was to assess the specificity of two assays for h. bilis immune response allowing for h. pylori, and their cross-reactivity in a population in thailand at high risk for cholangiocarcinoma. plasma samples from 92 thai volunteers ... | 2008 | 18596203 |
| differential susceptibility of p-glycoprotein deficient mice to colitis induction by environmental insults. | p-glycoprotein (p-gp), the product of the multidrug resistance gene (mdr), is an atp-dependent transmembrane pump, which is expressed in multiple cell lineages including epithelial and hematopoetic cells. the human mdr gene is located on chromosome 7 (7q21.1), a susceptibility loci for inflammatory bowel disease (ibd). a significant number of ibd patients carry mutations in this gene and p-gp-deficient fvb/n mice develop a severe spontaneous colitis, characterized by impaired intestinal barrier ... | 2009 | 19067430 |
| murine norovirus: an intercurrent variable in a mouse model of bacteria-induced inflammatory bowel disease. | murine norovirus (mnv) has recently been recognized as a widely prevalent viral pathogen in mouse colonies and causes disease and mortality in mice with impaired innate immunity. we tested the hypothesis that mnv infection would alter disease course and immune responses in mice with inflammatory bowel disease (ibd). fvb.129p2-abcb1a(tm1bor) n7 (mdr1a-/-) mice develop spontaneous ibd that is accelerated by infection with helicobacter bilis. as compared with controls, mdr1a-/- mice coinfected with ... | 2008 | 19149409 |
| concurrent helicobacter bilis infection in c57bl/6 mice attenuates proinflammatory h. pylori-induced gastric pathology. | because coinfections can alter helicobacter gastritis, we investigated whether enterohepatic helicobacter bilis modulates helicobacter pylori gastritis in c57bl/6 mice. thirty mice per group were sham dosed, h. bilis or h. pylori infected, or h. bilis infected followed in 2 weeks by h. pylori and then evaluated at 6 and 11 months postinfection (mpi) for gastritis and premalignant lesions. compared to h. pylori-infected mice, h. bilis/h. pylori-infected mice at 6 and 11 mpi had less severe gastri ... | 2009 | 19223483 |
| mucosal gene expression profiles following the colonization of immunocompetent defined-flora c3h mice with helicobacter bilis: a prelude to typhlocolitis. | an aberrant immune response to the commensal microbiota is widely hypothesized to contribute to the development of inflammatory bowel disease. helicobacter bilis colonization of defined-flora mice has been shown to trigger host immune responses to the commensal flora. however, the magnitude of the effects on mucosal homeostasis following colonization with h. bilis has not been determined. using microarray analysis, differential gene expression within the cecal mucosa was assessed at 15, 30, or 4 ... | 2009 | 19397886 |
| effect of helicobacter bilis infection on human bile duct cancer cells. | helicobacter pylori infection is known to be associated with chronic atrophic gastritis, peptic ulcers, and gastric malignancies. however, the effects of other helicobacter species have not been investigated extensively. in mice, a close relationship is observed between helicobacter hepaticus and hepatocellular carcinoma, and helicobacter species can be found in humans, most commonly in extragastric organs. there have also been reports that h. bilis may be associated with biliary malignancies in ... | 2010 | 19731027 |
| chronic hepatitis, hepatic dysplasia, fibrosis, and biliary hyperplasia in hamsters naturally infected with a novel helicobacter classified in the h. bilis cluster. | we recently described helicobacter-associated progressive, proliferative, and dysplastic typhlocolitis in aging (18- to 24-month-old) syrian hamsters. other pathogens associated with typhlocolitis in hamsters, clostridium difficile, lawsonia intracellularis, and giardia spp., were not indentified. the presence of helicobacter genus-specific dna was noted by pcr in cecal and paraffin-embedded liver samples from aged hamsters by the use of helicobacter-specific pcr primers. by 16s rrna analysis, t ... | 2009 | 19759229 |
| delineation of two helicobacter bilis genomospecies: implications for systematics and evolution. | the evolution and taxonomy of helicobacter bilis strains isolated in italy and finland were studied by phylogenetic analysis of different genes, comparative analysis of small rrna gene intervening sequence (ivs), amplified fragment length polymorphism analysis and dna-dna hybridization. the results of this study divided the h. bilis strains into two distinct and divergent genomic groups. in the absence of a specific phenotype or pathotype to distinguish these groups, however, they may be referre ... | 2010 | 19946059 |
| [latest advances in non-pylori helicobacter species]. | the genus helicobacter presently comprises 32 validly named species. helicobacter equorum, helicobacter baculiformis and helicobacter suis were recognized as new species in 2007 and 2008. non-pylori helicobacter species are occasionally isolated from human clinical specimens, though their natural hosts are mammals other than human and birds. it remains unclear whether they are associated with human diseases. 'helicobacter heilmannii' causes human chronic gastritis. several reports suggest that h ... | 2009 | 19999111 |
| helicobacter bilis colonization of the biliary system in patients with pancreaticobiliary maljunction. | helicobacter bilis is considered to be a causative factor in the pathogenesis of biliary cancer. this study investigated the prevalence of h. bilis colonization of the biliary system of patients with pancreaticobiliary maljunction (pbm). | 2010 | 20155791 |
| pyoderma gangrenosum-like ulcer in a patient with x-linked agammaglobulinemia: identification of helicobacter bilis by mass spectrometry analysis. | pyoderma gangrenosum-like ulcers and cellulitis of the lower extremities associated with recurrent fevers in patients with x-linked (bruton) agammaglobulinemia have been reported to be caused by helicobacter bilis (formerly classified as flexispira rappini and then helicobacter strain flexispira taxon 8). consistent themes in these reports are the difficulty in recovering this organism in blood and wound cultures and in maintaining isolates in vitro. we confirmed the presence of this organism in ... | 2010 | 20479300 |
| helicobacter bilis in human gallbladder cancer: results of a case-control study and a meta-analysis. | gallbladder cancer is an uncommon neoplasm of uncertain etiology and poor survival. recently, interest has been generated in bacterial infections and cancers. helicobacter is one such bacterium found to be associated with gastric maltoma, gastric adenocarcinoma and hepatobiliary neoplasms. | 2010 | 20843113 |
| could helicobacter organisms cause inflammatory bowel disease? | the discovery of helicobacter pylori sparked a revolution in the understanding and management of peptic ulcer disease and gastric cancer. other helicobacter species are recognized as important pathogenic agents in colitic diseases of rodents and primates, in particular helicobacter bilis, helicobacter fennelliae, helicobacter hepaticus and helicobacter trogontum. helicobacter bilis and h. hepaticus are now routinely used to initiate rodent models of inflammatory bowel disease (ibd), particularly ... | 2010 | 20955468 |
| helicobacter spp. other than helicobacter pylori. | over the last 12 months, new insights into the association of non-helicobacter pylori helicobacters with a range of human diseases in children and adults, including hepatobiliary disease, crohn's disease, sepsis, and gastric disease were published. studies investigating the presence of non-h. pylori helicobacters in domestic animals reinforce previous findings that cats and dogs harbor gastric helicobacter species and thus may be an important source of these organisms in humans. the confounding ... | 2010 | 21054656 |
| effect of heparin, fucoidan and other polysaccharides on adhesion of enterohepatic helicobacter species to murine macrophages. | helicobacter species have been isolated and cultured from both the gastric and enterohepatic niches of the gastrointestinal tract and are associated with a wide spectrum of diseases. some members of the enterohepatic helicobacter species (ehs), which include helicobacter bilis, helicobacter hepaticus and helicobacter pullorum, are associated with chronic inflammatory and proliferative bowel inflammation, hepatitis and in experimental murine studies with hepatic cancer. the present study aimed to ... | 2010 | 21088929 |
| noninvasive detection of inflammation-associated colon cancer in a mouse model. | helicobacter bilis-infected smad3(-/-) mice represent an attractive model of inflammation-associated colon cancer. most infected mice develop mucinous adenocarcinoma (muc) by 6 weeks post inoculation (pi); however, approximately one third do not progress to muc. the ability to predict the development of muc in mice used in therapeutic studies would confer a considerable saving of time and money. in addition, the inadvertent use of mice without muc may confound therapeutic studies by making treat ... | 2010 | 21170269 |
| helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger. | background: aberrant mucosal immune responses to antigens of the resident microbiota are a significant cause of inflammatory bowel diseases (ibd), as are genetic and environmental factors. previous work from our laboratory demonstrated that helicobacter bilis colonization of immunocompetent, defined microbiota mice induced antigen-specific immune responses to the resident microbiota, yet these mice failed to develop colitis, suggesting that the immunological provocation induced by h. bilis alone ... | 2011 | 21503679 |
| a case of helicobacter cinaedi bacteremia in an asplenic patient. | helicobacter cinaedi is an enterohepatic species. it can cause bacteremia, gastroenteritis, and cellulitis, particularly in immunocompromised individuals, such as those with acquired immunodeficiency syndrome, malignancy, or alcoholism. there are no previous reports of h. cinaedi infection in korea. a 71-yr-old man was admitted to the emergency room because of dyspnea on november 9, 2011. he had undergone splenectomy 3 yr ago because of immune hemolytic anemia. chest plain radiography revealed b ... | 2012 | 23130344 |
| evidence for conserved function of γ-glutamyltranspeptidase in helicobacter genus. | the confounding consequences of helicobacter bilis infection in experimental mice populations are well recognized, but the role of this bacterium in human diseases is less known. limited data are available on virulence determinants of this species. in helicobacter pylori, γ-glutamyltranspeptidase (γgt) contributes to the colonization of the gastric mucosa and to the pathogenesis of peptic ulcer. the role of γgt in h. bilis infections remains unknown. the annotated genome sequence of h. bilis rev ... | 2012 | 22348013 |
| role of campylobacter jejuni gamma-glutamyl transpeptidase on epithelial cell apoptosis and lymphocyte proliferation. | a gamma-glutamyl transpeptidase (ggt) is produced by up to 31% of strains of campylobacter jejuni isolates. c. jejuni ggt is close to helicobacter pylori ggt suggesting a conserved activity but unlike the latter, c. jejuni ggt has not been studied extensively. in line with the data available for h. pylori, our objectives were to purify c. jejuni ggt from the bacteria, and to evaluate its inhibitory and proapoptotic activities on epithelial cells and human lymphocytes. | 2014 | 24995041 |
| co-infection of the siberian hamster (phodopus sungorus) with a novel helicobacter sp. and campylobacter sp. | we report the isolation of a novel helicobacter isolated from the caecum of the siberian hamster (phodopus sungorus). sequence analysis showed 97% sequence similarity to helicobacter ganmani. in addition, we report the co-infection of these siberian hamsters with a campylobacter sp. and a second helicobacter sp. with 99% sequence similarity to helicobacter sp. flexispira taxon 8 (helicobacter bilis), a species isolated previously from patients with bacteraemia. gross necropsy and histopathology ... | 2015 | 25752854 |
| diagnosis of genus helicobacter through a hemi-nested pcr assay of 16s rrna. | the present study aimed to establish a genus-specific pcr-based assay to detect helicobacters using 16s rrna gene as the target template. we designed the hemi-nested primers based on sequences of 16s rrna gene of 34 types of helicobacter species. the inclusivity, sensitivity, and specificity of the pcr assay using these primers were examined in three different models, comprising feces simulated samples, blab/c mice infection model and clinic patients samples. the detection sensitivity of helicob ... | 2016 | 27275113 |
| rapid identification and subtyping of helicobacter cinaedi strains by intact-cell mass spectrometry profiling with the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. | helicobacter cinaedi infection is recognized as an increasingly important emerging disease in humans. although h. cinaedi-like strains have been isolated from a variety of animals, it is difficult to identify particular isolates due to their unusual phenotypic profiles and the limited number of biochemical tests for detecting helicobacters. moreover, analyses of the 16s rrna gene sequences are also limited due to the high levels of similarity among closely related helicobacters. this study was c ... | 2014 | 24153128 |
| association between helicobacter spp. infections and hepatobiliary malignancies: a review. | hepatobiliary cancers are highly lethal cancers that comprise a spectrum of invasive carcinomas originating in the liver hepatocellular carcinoma, the bile ducts intrahepatic cholangiocarcinoma and extrahepatic cholangiocarcinoma, the gallbladder and the ampulla of vater (collectively known as biliary tract cancers). these tumors account for approximately 13% of all annual cancer-related deaths worldwide and for 10%-20% of deaths from hepatobiliary malignancies. cholangiocarcinoma (cca) is a dev ... | 2015 | 25663761 |
| colitis and colon cancer in wasp-deficient mice require helicobacter species. | wiskott-aldrich syndrome protein-deficient patients and mice are immunodeficient and can develop inflammatory bowel disease. the intestinal microbiome is critical to the development of colitis in most animal models, in which helicobacter spp. have been implicated in disease pathogenesis. we sought to determine the role of helicobacter spp. in colitis development in wiskott-aldrich syndrome protein-deficient (wko) mice. | 2013 | 23820270 |
| [role of infectious agents in the emergence of malignant tumors]. | according to the data of the international agency for research on cancer (iarc), at least 6 virus species (hpv, ebv, hhv-8/kshv, htlv-1, hbv, hcv), 4 helminthes species (schistosoma haematobium and japonicum, opisthorchis viverrini, clonorchis sinensis) and i bacterium species (helicobacter pylori) have been proved to be capable of causing the development of cancer. the analysis of the data available shows that merkel cell polyomavirus (mcv), herpes simplex virus (hsv), john cunningham polyomavi ... | 2012 | 23163048 |
| helicobacter bilis infection alters mucosal bacteria and modulates colitis development in defined microbiota mice. | helicobacter bilis infection of c3h/hen mice harboring the altered schaedler flora (asf) triggers progressive immune responsiveness and the development of colitis. we sought to investigate temporal alterations in community structure of a defined (asf-colonized) microbiota in normal and inflamed murine intestines and to correlate microbiota changes to histopathologic lesions. | 2016 | 27755267 |
| helicobacter bilis and helicobacter trogontum: infectious causes of abortion in sheep. | the aim of our study was to determine the association of helicobacter spp. that had flexispira morphology with ovine abortion, and to understand the importance of these organisms as a cause of ovine abortion in new zealand. a retrospective diagnostic survey was carried out on laboratory submissions from ovine abortion outbreaks. a comparison was made of the proportion of laboratory submissions where helicobacter spp. were detected from flocks that had no other agent identified (group a) with a g ... | 2016 | 27016722 |
| male syrian hamsters experimentally infected with helicobacter spp. of the h. bilis cluster develop malt-associated gastrointestinal lymphomas. | aged hamsters naturally infected with novel helicobacter spp. classified in the h. bilis cluster develop hepatobiliary lesions and typhlocolitis. | 2016 | 26348390 |
| infection with helicobacter bilis but not helicobacter hepaticus was associated with extrahepatic cholangiocarcinoma. | the biliary tract cancer or cholangiocarcinoma (cca) represents the sixth leading cause of gastrointestinal tumors in the western world, and mortality varies across the world, with regions such as chile, thailand, japan, and northeastern india presenting the highest rates. cca may develop in the bile duct, gallbladder, or ampulla of vater; and risk factors include obesity, parity, genetic background, geographical and environmental factors. inflammation induced by bacterial infections might play ... | 2015 | 25582431 |
| infection with murine norovirus 4 does not alter helicobacter-induced inflammatory bowel disease in il10(-/-) mice. | infection of laboratory mice with murine noroviruses (mnv) is widely prevalent. mnv alters various mouse models of disease, including the helicobacter bilis-induced mouse model of inflammatory bowel disease (ibd) in mdr1a(--) mice. to further characterize the effect of mnv on ibd, we used mice deficient in the immunoregulatory cytokine il10 (il10(-/-) mice). in vitro infection of il10(-/-) bone marrow-derived macrophages (bmdm) with mnv4 cocultured with h. bilis antigens increased the gene expre ... | 2014 | 25296012 |
| increased dietary vitamin d suppresses mapk signaling, colitis, and colon cancer. | epidemiologic studies associate low serum vitamin d levels with an increased risk of colon cancer and inflammatory diseases such as inflammatory bowel disease (ibd). 129-smad3(tm1par)/j (smad3(-/-)) mice are a model of bacteria-driven colitis and colon cancer when infected with helicobacter bilis (h. bilis). thus, we used this mouse model to determine whether increased dietary vitamin d would reduce inflammation and colon cancer. smad3(-/-) mice were fed purified diet with either maintenance (1 ... | 2014 | 24938764 |
| standard operating procedures for maintaining cleanliness in a novel compact facility for breeding spf mice. | a compact facility for spf mice that was not equipped with a large autoclave used disposable mouse cages instead. the spf clean room was 5.7 × 8.1 × 2.7 m(3), with a breeding capacity of 1008 cages (168 cages on each of 6 racks). we evaluated cleanliness in the spf clean room under the conditions of an occupation rate of 60% to 70% and typically 1 to 3 personnel (maximum, 4 to 6) daily on weekdays. personnel were taught standard procedures and received training beforehand. during the 15-mo study ... | 2013 | 24351759 |
| gastric and enterohepatic non-helicobacter pylori helicobacters. | a substantial number of reports published in the last year have contributed to a better understanding of both human and animal infection with non-helicobacter pylori helicobacter species (nhph). gastric infection of humans with helicobacter suis and helicobacter felis as well as unidentified nhph has been described to cause a chronic gastritis and a variety of clinical symptoms, whereas enterohepatic nhph, including helicobacter cinaedi, helicobacter bilis, and helicobacter canis, have been repo ... | 2013 | 24011248 |
| helicobacter bilis gamma-glutamyltranspeptidase enhances inflammatory stress response via oxidative stress in colon epithelial cells. | helicobacter bilis (h. bilis) infection is associated with cases of inflammatory bowel disease, thyphlocolitis, hepatitis and cholecystitis. however, little is known about the bacterial virulence determinants or the molecular mechanisms involved. recently, h. bilis γ-glutamyltranspeptidase (hbggt) was shown to be a virulence factor decreasing host cell viability. bacterial ggts play a key role in synthesis and degradation of glutathione and enables the bacteria to utilize extracellular glutamine ... | 2013 | 24009737 |
| histopathological studies on cases of chronic mouse hepatitis by natural helicobacter infections. | it is known that helicobacter hepaticus or helicobacter bilis infection causes chronic inflammation of the colon and liver. chronic active hepatitis was found in radiation exposure experiments using male c3h/henrs mice at our institute. histopathologically, 103 cases among 978 mice (64-91 weeks of age at autopsy) had hepatic lesions regardless of irradiation exposure. mild lesions showed only focal necrosis and focal inflammation in the liver. severe cases were accompanied by hepatocytomegaly, b ... | 2013 | 23615172 |
| infections of helicobacter spp. in the biliary system are associated with biliary tract cancer: a meta-analysis. | as helicobacter spp. have been successfully isolated from the biliary system, a hypothetical question was raised about the role of these organisms in the development of biliary tract cancer. this meta-analysis has been carried out to explore the association between helicobacter spp. infection and biliary tract cancer. | 2013 | 23470268 |
| interleukin-7 receptor blockade suppresses adaptive and innate inflammatory responses in experimental colitis. | | 2012 | 23057802 |