| nocardia pseudobrasiliensis sp. nov., a new species of nocardia which groups bacterial strains previously identified as nocardia brasiliensis and associated with invasive diseases. | we studied five strains of a new nocardia taxon recently identified among nocardia brasiliensis strains associated with invasive diseases (r. j. wallace, jr., b. a. brown, z. blacklock, r. ulrich, k. jost, j. m. brown, m. m. mcneil, g. onyi, v. a. steingrube, and j. gibson, j. clin. microbiol. 33:1528-1533, 1995) to determine their taxonomic status. several characteristics of these organisms, including the presence of chemotype iv cell walls, nocardomycolic acids, a predominant menaquinone simil ... | 1996 | 8573505 |
| nocardia inohanensis sp. nov., nocardia yamanashiensis sp. nov. and nocardia niigatensis sp. nov., isolated from clinical specimens. | comparative 16s rdna studies on six strains of actinomycete isolated from clinical specimens revealed that they belong to the genus nocardia and are closely related to nocardia seriolae, nocardia otitidiscaviarum, nocardia uniformis, nocardia pseudobrasiliensis and nocardia crassostreae. however, the novel organisms consistently formed a clade distinct from that of the five latter species. determination of dna-dna relatedness indicated that these strains could be classified under three novel spe ... | 2004 | 15023976 |
| nocardiosis in large yellow croaker, larimichthys crocea (richardson). | an epizootic in seawater-cage reared large yellow croaker, larimichthys crocea, in china was caused by a nocardia sp. from august to october 2003. the cumulative mortality rate was 15% and the diseased fish were 16 months old with individual length varying from 25 to 30 cm. multiple, white nodules, 0.1-0.2 cm in diameter, were scattered on the heart, spleen and kidney. the morphology of isolated bacteria from lowenstein-jensen medium and tryptic soy agar was bead-like or long, slender, filamento ... | 2005 | 15960657 |
| nocardia concava sp. nov., isolated from japanese patients. | two actinomycete strains, ifm 0354(t) and ifm 0576, isolated from japanese patients, were found to have morphological, biochemical and chemotaxonomic properties consistent with their classification in the genus nocardia. the strains resembled nocardia otitidiscaviarum and nocardia uniformis in their phenotypic properties. comparative 16s rrna gene sequence analysis showed that the strains are closely related to nocardia seriolae. dna-dna relatedness values and phenotypic differences from n. seri ... | 2005 | 16166713 |
| loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens. | fish and shellfish diseases are a constant threat to the sustainability and economic viability of aquaculture. early diagnosis plays a vital role in management of fish and shellfish diseases. traditionally, various biochemical and serological tests have been used for fish disease diagnosis. however, the time and expertise required for such diagnoses makes it difficult for aquaculturists to easily adopt them under production conditions. polymerase chain reaction and probe-based nucleic acid detec ... | 2005 | 16302951 |
| detection of fish nocardiosis by loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) is a novel method that amplifies dna with high specificity and rapidity under isothermal conditions. in this study, using the lamp method, a protocol for detecting nocardia seriolae which is a causative agent of fish nocardiosis, was designed. | 2006 | 16696687 |
| experimental induction of nocardiosis in yellowtail, seriola quinqueradiata temminck and schlegel by artificial challenge. | challenge methods for inducing nocardiosis, caused by nocardia seriolae in yellowtail, seriola quinqueradiata, were evaluated. the first method involved intraperitoneal injection of 1.1 x 10(2), x 10(3) and x 10(4) cfu of n. seriolae; the second was by bath immersion with three different concentrations of bacterial suspension for 10 min; the third was by intradermal injection using a multipuncture device; the fourth was by oral administration using a tube; the fifth was based on the co-habitatio ... | 2006 | 16948702 |
| genetic and phenotypic comparison of nocardia seriolae isolated from fish in japan. | the phenotypic and genetic characterizations of 58 isolates of the fish pathogen nocardia seriolae, from amberjack, seriolae dumerili, yellowtail, seriola quinqueradiata, japanese flounder, paralichthys olivaceus, and chub mackerel, scomber japonicus, in japan from 1970-2005, were examined to investigate the epidemiological relationship between isolates. the phenotypic and genetic characterizations were determined by alpha-glucosidase activity and biased sinusoidal field gel electrophoresis (bsf ... | 2008 | 18482385 |
| characterization of the bpi-like gene from a subtracted cdna library of large yellow croaker (pseudosciaena crocea) and induced expression by formalin-inactivated vibrio alginolyticus and nocardia seriolae vaccine challenges. | one expressed sequence tag (est 64lf004 clone), which is from the subtracted cdna library of the head kidney of large yellow croaker (pseudosciaena crocea) stimulated with peptidoglycan (pg) by suppression subtractive hybridization (ssh) method, was cloned using race-pcr. the full length cdna, which possesses typical structural features of a signal peptide, a conserved lps binding domain and two bactericidal permeability-increasing (bpi) motifs as in higher vertebrates, was identified as a novel ... | 2008 | 18952461 |
| nocardia seriolae infection in the three striped tigerfish, terapon jarbua (forsskål). | an epizootic in pond cultured three striped tigerfish, terapon jarbua, in taiwan was caused by nocardia seriolae. diseased fish first showed clinical signs and mortalities in february and march 2003. the cumulative mortality within 2 months was 2.4% (1200 of 50 000) and affected fish were 7 months old with total lengths from 18 to 25 cm. most affected fish were pale and lethargic with haemorrhages and ulcers on the skin. the most significant gross pathological changes were varying degrees of asc ... | 2009 | 19335609 |
| expression of cytokine genes in head kidney and spleen cells of japanese flounder (paralichthys olivaceus) infected with nocardia seriolae. | nocardiosis caused by nocardia seriolae is an important disease affecting marine fish for which neither control nor preventive measures are available. in this study, we investigated cytokine gene expression in japanese flounder (paralichthys olivaceus) infected with n. seriolae to understand the innate immune response. japanese flounder were challenged with different concentrations of n. seriolae suspensions (0, 1, and 10 mg/l) by immersion for 10min. mortality was 75% and 95% in fish infected b ... | 2010 | 19819029 |
| immunohistochemical and taqman real-time pcr detection of mycobacterial infections in fish. | real-time pcr and immunohistochemistry (ihc) assays were developed to detect fish mycobacterial infections at the genus level, based on the rna polymerase ß subunit (rpob) gene and polyclonal anti-mycobacterium rabbit serum, respectively. the pcr assay positively identified a number of pathogenic mycobacteria including mycobacterium abscessus, m. avium ssp. avium, m. bohemicum, m. chelonae ssp. chelonae, m. farcinogenes, m. flavescens, m. fortuitum ssp. fortuitum, m. gastri, m. gordonae, m. immu ... | 2011 | 21306590 |
| mycobacterium bovis bcg vaccine induces non-specific immune responses in japanese flounder against nocardia seriolae. | nocardiosis caused by nocardia seriolae has been causing severe loss of fish production, so that an effective vaccine is urgently needed. mycobacterium bovis bcg (bcg) is a live attenuated vaccine for tuberculosis, which is effective against various infectious diseases including nocardiosis in mammals. in this study, the protective efficacy of bcg against n. seriolae was evaluated in japanese flounder paralichthys olivaceus and antigen-specific immune responses induced in bcg vaccinated fish wer ... | 2012 | 22609413 |
| analysis of the complete genome sequence of nocardia seriolae utf1, the causative agent of fish nocardiosis: the first reference genome sequence of the fish pathogenic nocardia species. | nocardiosis caused by nocardia seriolae is one of the major threats in the aquaculture of seriola species (yellowtail; s. quinqueradiata, amberjack; s. dumerili and kingfish; s. lalandi) in japan. here, we report the complete nucleotide genome sequence of n. seriolae utf1, isolated from a cultured yellowtail. the genome is a circular chromosome of 8,121,733 bp with a g+c content of 68.1% that encodes 7,697 predicted proteins. in the n. seriolae utf1 predicted genes, we found orthologs of virulen ... | 2017 | 28257489 |
| identification, molecular cloning of il-1β and its expression profile during nocardia seriolae infection in largemouth bass, micropterus salmoides. | in the present study, il-1β cdna was identified and analyzed from largemouth bass (micropterus salmoides). full length il-1β mrna was obtained using rapid amplification of cdna ends (race), which contains 78 bp 3'-utr, a 455 bp 5'-utr, and an open reading frame (orf) of 702 bp coding for 233 amino acid residues. the molecular weight and theoretical isoelectric point of largemouth bass il-1β protein was predicted to be 26.7 kda and 6.08 respectively. a largemouth bass il-1β phylogenetic analysis ... | 2016 | 27706080 |
| de novo transcriptome analysis of differential functional gene expression in largemouth bass (micropterus salmoides) after challenge with nocardia seriolae. | largemouth bass (micropterus salmoides) are common hosts of an epizootic bacterial infection by nocardia seriolae. we conducted transcriptome profiling of m. salmoides to understand the host immune response to n. seriolae infection, using the illumina sequencing platform. de novo assembly of paired-end reads yielded 47,881 unigenes, the total length, average length, n50, and gc content of which were 49,734,288, 1038, 1983 bp, and 45.94%, respectively. annotation was performed by comparison again ... | 2016 | 27529219 |
| isolation and genetic characterization of nocardia seriolae from snubnose pompano trachinotus blochii in vietnam. | a total of 480 cage-cultured fish were collected from 4 coastal provinces in central vietnam to investigate the causative agent of nocardiosis. fish displayed unique characteristics such as paleness and lethargy and exhibited haemorrhages and ulcers on the skin. prominent white nodules varying in size were observed in the spleen, kidney, and liver. furthermore, histopathological sections showed typical granulomatous lesions in these organs. using the ziehl-neelsen staining method, isolated bacte ... | 2016 | 27409241 |
| identification and functional characterization of multiple interleukin 12 in amberjack (seriola dumerili). | interleukin (il) -12 is a heterodimeric cytokine mainly produced by monocytes, macrophages, and dendritic cells in mammals. il-12p70 composed of il-12p35 and il-12p40, is known to play a crucial role in promoting cell-mediated immunity (cmi) through th1 differentiation and ifn-γ production. although two types of il-12p35 (p35a, p35b) and three types of il-12p40 (p40a, p40b and p40c) have been identified in several fish species, the knowledge on functional characteristics of teleost il-12 is stil ... | 2016 | 27238429 |
| development of vaccines against nocardiosis in fishes. | nocardiosis, one of the most systemic and devastating diseases, is currently emerging as an important disease of cultured marine and freshwater fishes. the causative agent of this disease is nocardia seriolae, a gram-positive acid-fast bacterium. an effective vaccine/vaccination strategy against this pathogen is necessary to control the significant loss in aquaculture practices. in this chapter, we present the vaccination/immunization protocol in fish against both live (sublethal) and inactivate ... | 2016 | 27076299 |
| draft genome sequence of erythromycin- and oxytetracycline-sensitive nocardia seriolae strain u-1 (nbrc 110359). | in japan, the emergence of macrolide- and oxytetracycline-resistant strains of nocardia seriolae has previously been reported. here, we describe the draft genome sequence of n. seriolae strain u-1, isolated in 2011 from a diseased yellowtail in kagoshima prefecture. the draft genome does not have any genes responsible for macrolide and tetracycline resistance. | 2016 | 26798107 |
| draft genome sequence of nocardia seriolae strain n-2927 (nbrc 110360), isolated as the causal agent of nocardiosis of yellowtail (seriola quinqueradiata) in kochi prefecture, japan. | we report the draft genome sequence of nocardia seriolae strain n-2927 (nbrc 110360), isolated from cultured yellowtail seriola quinqueradiata. rast annotation of the genome revealed 117 genes involved in the virulence, disease, and defense subsystem. eleven of these genes were predicted as antibiotic resistance genes. | 2015 | 25767227 |
| draft genome sequence of nocardia seriolae zj0503, a fish pathogen isolated from trachinotus ovatus in china. | nocardia seriolae is a pathogen that causes nocardiosis in marine and freshwater fish. here, we report the draft genome sequence of n. seriolae strain zj0503, which was isolated from trachinotus ovatus in guangdong, china. | 2015 | 25555736 |
| development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals. | rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ... | 2014 | 24954661 |
| immune responses to live and inactivated nocardia seriolae and protective effect of recombinant interferon gamma (rifn γ) against nocardiosis in ginbuna crucian carp, carassius auratus langsdorfii. | looking into the fact that substantial mortality and morbidity is associated with intracellular gram +ve bacterium, nocardia seriolae infection, an effective vaccine against this pathogen is necessary to control the significant losses in aquaculture practices. therefore, an attempt was made to evaluate the effect of live (sub-lethal) and inactivated (antigenic form) n. seriolae on cellular and humoral immunity in ginbuna crucian carp, carassius auratus langsdorfii as well as the therapeutic pote ... | 2014 | 24882019 |
| adjuvant effect of recombinant interleukin-12 in the nocardiosis formalin-killed vaccine of the amberjack seriola dumerili. | nocardiosis causes serious economic damage in the fish farming of japanese yellowtail fish. nocardia seriolae identified as pathogenic bacterium is an intracellular-pathogen. in general, induction of cell-mediated immunity (cmi) is effective in infection defense against intracellular-pathogen. however, the conventional formalin-killed n. seriolae (fkc) vaccine induces humoral immunity. interleukin-12 (il-12) is th1 type heterodimeric cytokine and induces cell differentiation in mammals. our prev ... | 2017 | 28602739 |