| antibodies to marine caliciviruses in the steller sea lion (eumetopias jubatus schreber). | sera from 145 steller sea lions (76 adults, three subadults, 37 pups, and 29 fetuses) were tested for neutralizing antibodies to nine marine calicivirus serotypes. antibodies were found to san miguel sea lion virus (smsv) types 1, 5, 6, 7, 8, 10 and 13, and to tillamook (bovine) calicivirus, but no antibodies were found to the walrus calicivirus. titers (microtiter neutralization assay) ranged from 1:20 to 1:320, with many positive reactions at the higher dilutions (greater than or equal to 1:80 ... | 1987 | 3820427 |
| prevalence and distribution of serum neutralizing antibodies to tillamook (bovine) calicivirus in selected populations of marine mammals. | neutralizing antibodies to tillamook calicivirus (tcv) were found in sera collected from california sea lions (zalophus c. californianus lesson) in 1983 and 1984 and in sera collected from steller sea lions (eumetopias jubatus schreber) in 1976 and 1985. the combined prevalence of antibodies for these two species was 10/228 = 4.38%. titers ranged from 1:20 (five animals), to 1:40 (four animals), to 1:80 (one animal) by standard microtiter neutralization assay. the seropositive pinnipeds were dis ... | 1987 | 3820428 |
| characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus. | bovine enteric caliciviruses (bec) are associated with diarrhea in young calves. the bec strains detected in europe form a third genogroup within the genus "norwalk-like viruses" (nlv) of the family caliciviridae. in this report, we present sequence, clinical, and histological data characterizing a novel enteropathogenic bec strain, nb, detected in fecal specimens from calves in the united states. the complete rna genome of the nb virus is 7,453 bases long and is organized into two open reading ... | 2002 | 12239283 |
| evaluation of a p30 gene-based real-time reverse transcriptase polymerase chain reaction assay for detection of feline caliciviruses. | this report describes a feline calicivirus (fcv) p30 gene-based real-time sybr green i reverse transcriptase polymerase chain reaction (rt-pcr) assay that is capable of detecting low virus concentrations and a broad range of fcv isolates. the assay consisted of a 1-step rt-pcr reaction with primers delineating a 126-base-pair (bp) region of the fcv p30 gene. sensitivity of the rt-pcr assay was determined to be equivalent to a fcv titer of 1.2 x 10(1) to 1.2 x 10(2) tcid50/ml. the assay was linea ... | 2004 | 14765747 |