| impaired renal function in owl monkeys (aotus nancymai) infected with plasmodium falciparum. | impaired renal function was observed in sixteen aotus nancymai 25 and 3 months following infection with the uganda palo alto strain of plasmodium falciparum. decrease were noted in the clearance of endogenous creatinine, creatinine excretion, and urine volume while increases were observed in serum urea nitrogen, urine protein, urine potassium, fractional excretion of phosphorus and potassium, and activities of urinary enzymes. the results were suggestive of glomerulonephropathy and chronic renal ... | 1992 | 1343724 |
| platelet kinetics and other hematological profiles in experimental plasmodium falciparum infection: a comparative study between saimiri and aotus monkeys. | levels of platelets and other hematological values were monitored in 21 saimiri and 12 aotus monkeys over a period of three weeks post-infection with monkey-adapted indochina cdc-1 strain of plasmodium falciparum. in both saimiri sciureus boliviensis and aotus nancymai karyotype-1 monkeys the severest thrombocytopenia was observed at 14 days post-infection coinciding with peak parasitemia, neutropenia, lymphocytosis, and anemia associated with severe hemoglobinemia and elevated fibrinogen degene ... | 1992 | 1420030 |
| development of a polymorphic strain of plasmodium vivax in monkeys. | a strain of plasmodium vivax from thailand with a polymorphic repeat unit of the circumsporozoite protein was established in saimiri sciureus boliviensis and 3 species of aotus monkeys. all 11 attempts to transmit infection via sporozoite inoculation, 4 times to splenectomized s. sciureus boliviensis, 2 times to splenectomized aotus nancymai, and 5 times to intact saimiri monkeys, were successful. anopheles freeborni, anopheles stephensi, anopheles dirus, and anopheles gambiae mosquitoes were in ... | 1992 | 1597793 |
| immunogenicity and efficacy trials in aotus nancymai monkeys with model compounds representing parts of a 75-kd merozoite surface antigen of plasmodium falciparum. | we tested the ability of a recombinant dna-encoded fragment (c7ag) of a plasmodium falciparum merozoite protein (p75) and of two carrier-free peptide models (28-mer and 76-mer) to stimulate boostable antibody responses in aotus nancymai monkeys. in addition, we evaluated protection against challenge with the uganda palo alto (fup) strain of this parasite. the data indicate that c7ag elicited a strong and boostable igg antibody response in all the monkeys immunized. however, studies with the pept ... | 1992 | 1621894 |
| serum chemistry of the wild caught karyotype i night monkey (aotus nancymai). | serum samples from 254 wild-caught aotus nancymai were analyzed to determine the reference intervals for serum chemistry parameters in this species. findings show values of total bilirubin, creatinine, alkaline phosphatase, alanine aminotransferase, urea nitrogen, serum albumin, and gamma glutamic transpeptidase having a non-normal distribution. based on nonparametric tests, significant differences between male and female values were observed for cholesterol, serum calcium, and gamma glutamic tr ... | 1991 | 1658442 |
| spontaneous hydrocephalus in an adult owl monkey (aotus nancymai). | | 1991 | 1658453 |
| twinning in the karyotype i night monkey (aotus nancymai). | a case of successful survival of twins in a karyotype i (2n = 54) night monkey (aotus nancymai) is described. monthly weights of the pregnant female, and of the twins, are compared to those of pregnant females and their single birth offspring of the same sex and karyotype. | 1991 | 1787534 |
| immunization of owl monkeys with the ring-infected erythrocyte surface antigen of plasmodium falciparum. | aotus nancymai were immunized with the 4-mer, 8-mer, and 11-mer repeat peptides of the ring-infected erythrocyte surface antigen molecule of plasmodium falciparum conjugated to diphtheria toxoid with muramyl dipeptide (mdp) as adjuvant. immunization failed to induce protective immunity against the uganda palo alto strain of p. falciparum as judged by maximum levels of parasitemia of immunized monkeys relative to those of controls. the fused polypeptide fpag632, when combined with mdp, also faile ... | 1991 | 1996739 |
| mortality and body weight changes in aotus nancymai shipped from iquitos, peru to richland, washington. | four hundred and fifty wild-caught owl monkeys (aotus nancymai) were shipped from iquitos, peru to richland, washington. mortality and body weight changes from the time of shipment to 30 days after arrival were evaluated using age, sex, and transit time as comparison criteria. no association between mortality and sex, age, or transit time was observed. the mortality percentage from shipment to 30 days after arrival was 2.44%. both age and transit time had a significant effect on weight changes, ... | 1991 | 2051470 |
| immunogenicity and protective efficacy of affinity-purified plasmodium falciparum exoantigens in aotus nancymai monkeys. | soluble plasmodium falciparum polypeptides, affinity-purified from culture supernatant fluids using sequential immunoadsorptions employing both monoclonal and polyclonal antibodies, induced protective immunity against experimental falciparum malaria in peruvian aotus nancymai monkeys. susceptible monkeys were vaccinated with polypeptides affinity-purified from supernatant fluids of p. falciparum indochina i/cdc cultures. eighteen animals (6 immunized with purified antigens plus adjuvants, 6 inje ... | 1991 | 2052857 |
| hematology of the wild caught karyotype 1 owl monkey (aotus nancymai). | | 1990 | 2157105 |
| relationship of serum total calcium and albumin and total protein in owl monkeys (aotus nancymai). | a positive linear relationship was found between total calcium and albumin and between total calcium and total protein in the serum of 205 owl monkeys. adjustment formulas for calcium were derived: adjusted calcium (mg/dl) = measured calcium (mg/dl) - 0.84 [albumin (g/dl)] + 3.8 and adjusted calcium (mg/dl) = measured calcium (mg/dl) - 0.82 [total protein (g/dl)] + 6.5. adjusted calcium calculations for monkeys based on albumin concentration were similar to those for man and dogs, but different ... | 1990 | 2213852 |
| immunization of owl monkeys with a combination of plasmodium falciparum asexual blood-stage synthetic peptide antigens. | a mixture of 3 synthetic peptides (35.1, 55.1, and 83.1) corresponding to portions of the 35 kda, 55 kda, and 83 kda proteins from the asexual blood stages of plasmodium falciparum and a polymer of a syntheic peptide incorporating the 3 individual peptides (spf66) were tested as candidate malaria vaccine antigens in aotus nancymai. monkeys were immunized with combinations of the 3 peptides from 2 separate sources (centers for disease control [cdc], atlanta, ga or colombia) or with the synthetic ... | 1990 | 2240363 |
| hypercalcemia and disseminated histoplasmosis in an owl monkey. | an adult male owl monkey (aotus nancymai) had hypercalcemia associated with a disseminated case of histoplasmosis (histoplasma capsulatum var. capsulatum). histopathologic examination of tissues at necropsy showed moderate to marked numbers of small granulomas involving multiple organs. the granulomas contained numerous round, basophilic, intracellular structures, 1-2 microns in diameter, that stained positive in periodic acid-schiff and gomori's methenamine silver tests. the hypercalcemia in th ... | 1990 | 2254918 |
| infection of peruvian aotus nancymai monkeys with different strains of plasmodium falciparum, p. vivax, and p. malariae. | aotus nancymai (karyotype i) monkeys from peru were studied for their susceptibility to infection with plasmodium falciparum, p. vivax, and p. malariae. three strains of p. falciparum (santa lucia from el salvador, indochina i/cdc from thailand, and uganda palo alto) were inoculated into 38 monkeys. the results indicated that this species of aotus monkey is highly susceptible to infection. the uganda palo alto and the santa lucia strain parasites appear to be the most useful for immunologic and ... | 1988 | 3288739 |
| plasmodium vivax: in vitro growth and reinvasion in red blood cells of aotus nancymai. | plasmodium vivax was maintained in experimentally infected aotus nancymai. positive monkeys were used as donors for culture material. after leucocyte removal with two different methods, including the classic cf11 method and a commercially available filter, parasites were grown under continuous shaking conditions in standard rpmi 1640, containing 20% human ab + serum. when mature schizonts were present, artificially induced reticulocytes from monkeys pretreated with the hemolytic drug phenylhydra ... | 1988 | 3294025 |
| transmission of plasmodium simium to aotus nancymai, a. vociferans, a. azarae boliviensis, and saimiri sciureus boliviensis monkeys. | | 1987 | 3598812 |
| selection of different strains of plasmodium falciparum for testing blood-stage vaccines in aotus nancymai monkeys. | three strains of plasmodium falciparum, vietnam oak knoll (fvo), uganda palo alto (hawaii) (fup-h) and uganda palo alto (cayenne) (fup-c), were examined in 154 aotus nancymai monkeys as suitable models for testing blood-stage vaccines. the vietnam oak knoll strain had the greatest number of animals with maximum parasite counts > 200,000/microliters. uniformity of the parasitemia curve increased from passage 4 to passage 6 with an accompanying decrease in the number of days required to reach maxi ... | 1994 | 8074257 |
| trypanosomes and microfilariae in feral owl and squirrel monkeys maintained in research colonies. | a group of 358 owl and squirrel monkeys imported from colombia, peru, and bolivia for the u.s. agency for international development malaria vaccine development program was examined for trypanosomes and microfilariae. trypanosoma rangeli, isolated by hemoculture from aotus nancymai, saimiri b. boliviensis, and s. b. peruviensis, accounted for 76.6% of all trypanosome infections. trypanosoma cruzi was isolated from 25 of 194 s. b. boliviensis, including two mixed infections with t. rangeli. identi ... | 1993 | 8357088 |
| immunization of aotus nancymai with recombinant c terminus of plasmodium falciparum merozoite surface protein 1 in liposomes and alum adjuvant does not induce protection against a challenge infection. | merozoite surface protein 1 (msp-1) of plasmodium falciparum is an antimalarial vaccine candidate. the highly conserved 19-kda c-terminal processing fragment of msp-1 (msp-1(19)) is of particular interest since it contains epitopes recognized by monoclonal antibodies which inhibit the invasion of erythrocytes in vitro. the presence of naturally acquired anti-msp-1(19) antibodies in individuals exposed to malaria has been correlated with reduced morbidity, and immunization with an equivalent reco ... | 1996 | 8751907 |
| use of olfactory cues in foraging by owl monkeys (aotus nancymai) and capuchin monkeys (cebus apella). | the authors tested free-ranging new world monkeys (nocturnal owl monkeys [aotus nancymai] and diurnal capuchin monkeys [cebus apella]) to determine the extent to which they use olfactory cues to locate food hidden in containers at 2 of 6 feeding sites within a 1 1/2-ha forested enclosure. these 2 sites were selected randomly for each trial and then were baited with banana and banana peel residue. the 4 other sites were unbaited and unscented. in trials in which the food was not visible to the mo ... | 1997 | 9170280 |
| baculovirus-mediated expression of plasmodium falciparum erythrocyte binding antigen 175 polypeptides and their recognition by human antibodies. | the erythrocyte binding antigen eba-175 is a 175-kda plasmodium falciparum protein which mediates merozoite invasion of erythrocytes in a sialic acid-dependent manner. the purpose of this study was to produce recombinant eba-175 polypeptide domains which have previously been identified as being involved in the interaction of eba-175 with erythrocytes and to determine whether these polypeptides are recognized by malaria-specific antibodies. the eba-175 gene was cloned by pcr from genomic dna isol ... | 1997 | 9284129 |
| adaptation of a strain of plasmodium vivax from mauritania to new world monkeys and anopheline mosquitoes. | a strain of plasmodium vivax from mauritania was adapted to develop in aotus lemurinus griseimembra, aotus nancymai, saimiri boliviensis, and hybrid aotus monkeys. infections were induced via the inoculation of sporozoites dissected from the salivary glands of anopheles gambiae, anopheles freeborni, and anopheles stephensi mosquitoes or the intravenous passage of infected erythrocytes. infections in 3 a. lemurinus griseimembra monkeys readily infected mosquitoes. four lines of the mauritania par ... | 1998 | 9645868 |
| sequence and diversity of t-cell receptor alpha v, j, and c genes of the owl monkey aotus nancymaae. | we cloned and sequenced tcr alpha chain cdna of three healthy aotus nancymaae monkeys. fifteen different traj segments and 9 different trav genes were identified in the 29 rearrangements analyzed. as expected from the greater phylogenetic distance, a. nancymaae tra gene sequences diverged more from the human sequences than those of the chimpanzee or the rhesus macaque. however, no aotus traj segment or trav gene was found which lacked a human counterpart. these counterparts were aj02, aj05, aj09 ... | 1998 | 9716644 |
| schistosoma mansoni infection in owl monkeys (aontus nancymai): evidence for the early elimination of adult worms. | detailed parasitologic, serologic, clinical and histopathologic studies were conducted in owl monkeys (aotus nancymai) exposed to varying numbers of cercariae of schistosoma mansoni. all the experimental animals had clinical symptoms suggestive of infection (weight loss diarrhoea, mucus in stools, etc.) which were not seen in uninfected individuals. the only a. vociferans included in this study passed s. mansoni eggs 8 weeks after infection. none of the a. nancymai passed eggs in their faeces. n ... | 1998 | 9777712 |
| passive transfer of growth-inhibitory antibodies raised against yeast-expressed recombinant plasmodium falciparum merozoite surface protein-1(19). | purified rabbit immunoglobulin raised against yeast-expressed recombinant fvo or 3d7 plasmodium falciparum merozoite surface protein-1 (msp-1) 19k-d c terminal fragment (msp-1(19)) was transfused into malaria-naive aotus nancymai monkeys that were immediately challenged with fvo asexual stage malaria parasites. control monkeys received rabbit immunoglobulin raised against the sexual stage antigen pfs25 or aotus hyperimmune serum obtained from monkeys immunized by p. falciparum infection and drug ... | 1998 | 9886211 |
| sequence and diversity of t-cell receptor beta-chain v and j genes of the owl monkey aotus nancymaae. | the new world primate aotus nancymaae is susceptible to infection with the human malaria parasite plasmodium falciparum and has therefore been recommended by the world health organization as a model for the evaluation of malaria vaccine candidates. recently, we have shown that aotus tcrva genes and tcrja segments exhibit a high degree of similarity to human counterparts. in the present report we used reverse transcription polymerase chain reaction to analyze the sequences of a. nancymaae tcr bet ... | 1999 | 10398806 |
| adaptation of the amru-1 strain of plasmodium vivax to aotus and saimiri monkeys and to four species of anopheline mosquitoes. | a chloroquine-resistant strain of plasmodium vivax (amru-1) from papua new guinea has been adapted to grow in 4 species of aotus monkeys (aotus lemurinus griseimembra, aotus vaciferans, aotus nancymai, and aotus azarae boliviensis), hybrid aotus monkeys, and saimiri boliviensis monkeys. whereas it was possible to infect saimiri monkeys with this parasite by inoculation of parasitized erythrocytes, only 42% of saimiri monkeys became infected, compared to 92% of aotus monkeys attempted. comparativ ... | 1999 | 10461947 |
| immunogenicity and efficacy in aotus monkeys of four recombinant plasmodium falciparum vaccines in multiple adjuvant formulations based on the 19-kilodalton c terminus of merozoite surface protein 1. | the immunogenicity and protective efficacy of four versions of recombinant c-terminal 19-kda epidermal growth factor-like region of the major surface protein 1 (rmsp1(19)) of plasmodium falciparum was studied in aotus monkeys. vaccination with each of the four rmsp1(19) constructs elicited high levels of antibodies to msp1(19) but only one construct, the 19-kda fragment expressed as a secreted fusion protein from saccharomyces cerevisiae (yp30p2msp1(19)), induced a high degree of protective immu ... | 2000 | 10722622 |
| immunoglobulin kappa light-chain v, j, and c gene sequences of the owl monkey aotus nancymaae. | sequences of aotus nancymaae immunoglobulin kappa light-chain rearrangements were analyzed after reverse transcription polymerase chain reaction. among 22 in-frame rearrangements analyzed, 12 igkv genes belonging to the families 1, 2, or 3 were identified. aotus counterparts for all five human igkj genes were found. the identity of the deduced human and aotus amino acid sequences was between 83% and 92% for junctional regions and 74% for the constant region. sequence comparisons between rearrang ... | 2000 | 10752631 |
| sequence and diversity of drb genes of aotus nancymaae, a primate model for human malaria parasites. | the new world primate aotus nancymaae is susceptible to infection with the human malaria parasite plasmodium falciparum and plasmodium vivax and has therefore been recommended by the world health organization as a model for evaluation of malaria vaccine candidates. we present here a first step in the molecular characterization of the major histocompatibility complex (mhc) class ii drb genes of aotus nancymaae (owl monkey or night monkey) by nucleotide sequence analysis of the polymorphic exon 2 ... | 2000 | 10752632 |
| conditionally replicating herpes simplex virus mutant, g207 for the treatment of malignant glioma: results of a phase i trial. | g207 is a conditionally replicating derivative of herpes simplex virus (hsv) type-1 strain f engineered with deletions of both gamma(1)34.5 loci and a lacz insertion disabling the ul39 gene. we have demonstrated the efficacy of g207 in treating malignant glial tumors in athymic mice, as well as the safety of intracerebral g207 inoculation in mice and in aotus nancymai. we sought to determine the safety of g207 inoculation into cerebral malignant glial tumors in humans. criteria for inclusion int ... | 2000 | 10845725 |
| sequence and diversity of mhc dqa and dqb genes of the owl monkey aotus nancymaae. | the new world primate aotus nancymaae has been recommended by the world health organization (who) as a model for evaluation of malaria vaccine candidates, given its susceptibility to experimental infection with the human malaria parasites plasmodium falciparum and plasmodium vivax. we present here the nucleotide sequences of the complete cdna of mhc-dqa1 and of the polymorphic exon 2 segments of mhc-dqb1/dqb2. in a group of three nonrelated animals captured in the wild, five alleles of mhc-dqa1 ... | 2000 | 10912504 |
| efficacy of two alternate vaccines based on plasmodium falciparum merozoite surface protein 1 in an aotus challenge trial. | in an attempt to produce a more defined, clinical-grade version of a vaccine based on plasmodium falciparum merozoite surface protein 1 (msp1), we evaluated the efficacy of two recombinant forms of msp1 in an aotus nancymai challenge model system. one recombinant vaccine, bvmsp1(42), based on the 42-kda c-terminal portion of msp1, was expressed as a secreted protein in baculovirus-infected insect cells. a highly pure baculovirus product could be reproducibly expressed and purified at yields in e ... | 2001 | 11179324 |
| adaptation of a chloroquine-resistant strain of plasmodium vivax from indonesia to new world monkeys. | the spread of chloroquine-resistant plasmodium vivax from papua new guinea and indonesia poses a serious health threat to areas of southeast asia where this species of malaria parasite is endemic. a strain of p. vivax from indonesia was adapted to develop in splenectomized aotus lemurinus griseimembra, aotus vociferans, aotus nancymai, and saimiri boliviensis monkeys. transmission to splenectomized saimiri monkeys was obtained via sporozoites. chemotherapeutic studies indicated that the strain w ... | 2000 | 11220765 |
| abundance, diversity, and patterns of distribution of primates on the tapiche river in amazonian peru. | this work presents data on the relative diversity, abundance, and distribution patterns of primates in a 20 km2 area of the tapiche river in the peruvian amazon. population data were collected while the study area was both inundated and dry (march to september 1997) using conventional line-transect census techniques. survey results reflected the presence of 11 primate species, but population parameters on only eight of the species will be presented, including saddleback tamarins (saguinus fuscic ... | 2001 | 11376449 |
| functional and structural similarity of v gamma 9v delta 2 t cells in humans and aotus monkeys, a primate infection model for plasmodium falciparum malaria. | gammadelta t cells are implicated to play crucial roles during early immune responses to pathogens. a subset of human gammadelta t cells carrying the vgamma9vdelta2 tcr recognize small, phosphorylated nonpeptidic ags. however, the precise role of these cells and the ligands recognized in human immune responses against pathogens remains unclear because of the lack of suitable animal models. we have analyzed the reactivity of spleen cells of the new world monkey aotus nancymaae against isopentenyl ... | 2001 | 11714808 |
| a recombinant vaccine expressed in the milk of transgenic mice protects aotus monkeys from a lethal challenge with plasmodium falciparum. | two strains of transgenic mice have been generated that secrete into their milk a malaria vaccine candidate, the 42-kda c-terminal portion of plasmodium falciparum merozoite surface protein 1 (msp1(42)). one strain secretes an msp1(42) with an amino acid sequence homologous to that of the fvo parasite line, the other an msp1(42) where two putative n-linked glycosylation sites in the fvo sequence have been removed. both forms of msp1(42) were purified from whole milk to greater than 91% homogenei ... | 2002 | 11752405 |
| merozoite surface protein 3 and protection against malaria in aotus nancymai monkeys. | a blood-stage vaccine based on plasmodium falciparum merozoite surface protein 3 (msp3) was tested for efficacy in a primate model. aotus nancymai monkeys were vaccinated with yeast-expressed msp3 before a lethal challenge with plasmodium falciparum parasites. five of 7 control monkeys had acute infections and required treatment to control parasitemia. only 1 of 7 monkeys vaccinated with msp3 required this treatment. the efficacy of the msp3 vaccination appeared to be comparable to that of msp1( ... | 2002 | 11865423 |
| infection of aotus and saimiri monkeys with plasmodium gonderi. | attempts were made to infect 4 species of new world monkeys (saimiri boliviensis, aotus nancymai, a. vociferans, a. azarae boliviensis) with plasmodium gonderi, a malaria parasite of african monkeys. sporozoites were obtained from anopheles dirus or a. stephensi mosquitoes that fed on an infected rhesus monkey (macaca mulatta). inoculation of sporozoites was by injection of dissected sporozoites by either the intravenous or intrahepatic routes, or by mosquito bite. liver biopsies done 7 or 8 day ... | 2002 | 12054031 |
| plasmodium vivax: parasitemia determination by real-time quantitative pcr in aotus monkeys. | plasmodium vivax and plasmodium falciparum are the two prevalent human malaria species. a colombian p. vivax wild strain has been adapted in aotus nancymaae monkeys for use in further biological and immunological studies. we present data validating a real-time pcr assay quantifying p. vivax parasitemia, using the small subunit ribosomal rna genes as an amplification target. p. vivax species-specific primers were designed on the 18s ribosomal gene v8 region, for amplifying both asexual and sporoz ... | 2002 | 12054703 |
| sequence and expression of mhc-dpb1 molecules of the new world monkey aotus nancymaae, a primate model for plasmodium falciparum. | aotus nancymaae represents an animal model for the pre-clinical evaluation of blood-stage vaccine candidates against plasmodium falciparum and plasmodium vivax. we present here the nucleotide sequences of exon 2 and 3 of mhc-dpb1 genes. in a group of seven unrelated animals captured in the wild, three alleles of mhc-dpb1 exon 2 could be identified. phylogenetic analysis shows that in contrast to aona- drb and - dqb, the aona-dpb1exon 2 amino acid sequences cluster in a species-specific manner. n ... | 2002 | 12136336 |
| potential of the panama strain of plasmodium vivax for the testing of malarial vaccines in aotus nancymai monkeys. | aotus monkeys were infected with a strain of plasmodium vivax from panama to determine its potential for the testing of malarial vaccines. after sporozoite inoculation, 3 splenectomized aotus nancymai that had been infected previously with plasmodium falciparum and p. vivax had prepatent periods of 13, 15, and 15 days with maximum parasite counts of 12,726/microl, 5,310/microl, and 9,180/microl. three other a. nancymai previously infected with p. falciparum only had prepatent periods of 17, 15, ... | 2002 | 12479543 |
| cytogenetic identification of a hybrid owl monkey, aotus nancymaae x aotus lemurinus griseimembra. | a neonate male owl monkey (aotus sp.) was identified cytogenetically as a hybrid after it failed to nurse and died. phenotypically, the male parent possessed characteristics of the "gray-neck group," and g-banded karyotypes identified him as aotus lemurinus griseimembra (2n = 53), heterozygous for the centric fusion of chromosomes 13 and 14. the female parent belonged to the "red-neck group" and was identified cytogenetically as aotus nancymaae (2n = 54). the neonate hybrid had 2n = 54 chromosom ... | 2001 | 12790410 |
| a highly infective plasmodium vivax strain adapted to aotus monkeys: quantitative haematological and molecular determinations useful for p. vivax malaria vaccine development. | the new world primate aotus nancymaae is susceptible to infection by the human malaria parasite plasmodium vivax and has therefore been recommended by the world health organization as a model for malaria vaccine candidate evaluation. we report the isolation, adaptation, titration and genetic characterization of a p. vivax wild strain in splenectomized a. nancymaae monkeys. parasitemia remained high after 22 passages, reaching 7.88% by giemsa and acridine orange staining and real-time pcr determi ... | 2003 | 12922128 |
| splenectomised and spleen intact aotus monkeys' immune response to plasmodium vivax msp-1 protein fragments and their high activity binding peptides. | two e. coli expressed recombinant polypeptides (rpvmsp-1(14) and rpvmsp-1(20)) contained in the 33kda fragment, located within plasmodium vivax merozoite surface protein (pvmsp-1) 42kda c-terminal region, and a cocktail of high reticulocyte binding synthetic peptides located within these fragments, were evaluated for immunogenicity and protective immune responses in splenectomised and spleen intact aotus nancymaae monkeys. thirty splenectomised monkeys who had been previously immunised with eith ... | 2003 | 14505893 |
| characterizing t-cell receptor gamma-variable gene in aotus nancymaae owl monkey peripheral blood. | gammadelta t lymphocytes have a heterodimeric complex formed by the association of gamma and delta chains as receptor. proliferation of this lymphocyte population has been observed, when infection by several pathogens such as mycobacterium tuberculosis and plasmodium spp. occurs. the new world monkey aotus nancymaae has become a very good experimental model for the immunological and physiopathological study of these infectious agents. the a. nancymaae gamma-variable region was characterized from ... | 2003 | 14617030 |
| orientating peptide residues and increasing the distance between pockets to enable fitting into mhc-tcr complex determine protection against malaria. | the erythrocyte binding antigen eba-175 is a 175-kda plasmodium falciparum protein, which has been shown to be involved in the process of invasion of erythrocytes. it has been found that conserved peptide 1818 belonging to this protein has high red blood cell binding capacity and plays an important role in the invasion process. this peptide is neither immunogenic nor protective. peptide 1818 analogues had some of their previously recognized critical red blood cell binding residues substituted fo ... | 2004 | 15157087 |
| comparative analysis of cd45 proteins in primate context: owl monkeys vs humans. | transmembrane protein tyrosine phosphatase (ptpase) cd45 has been implicated in activating, differentiating and the development of different immune system cells. it regulates t-or b-cell activation during receptor-specific recognition by dephosphorylating tyrosine residues in protein kinase substrates. aotus nancymaae, aotus nigriceps, and aotus vociferans cd45 nucleotide and deduced amino acid sequences are presented here, where we found 90-92% identity with the human counterpart in the nucleot ... | 2004 | 15245371 |
| identifying and structurally characterizing cd1b in aotus nancymaae owl monkeys. | this study reports the molecular characterization and tissue expression of the non-human aotus nancymaae primate cd1b isoform in the search for an experimental animal model to be used in evaluating the role of non-peptide antigen-presentation molecules in the immune response to infectious agents. cd1b expression on the surface of a. nancymaae peripheral blood monocyte-derived dendritic cells, shown by flow cytometry, was made possible by using human cd1b isoform antibodies. studying the expressi ... | 2004 | 15365647 |
| detection of a plasmodium vivax erythrocyte binding protein by flow cytometry. | the malaria parasite plasmodium vivax preferentially invades reticulocytes. it is therefore relevant for vaccine development purposes to identify and characterize p. vivax proteins that bind specifically to the surface of reticulocytes. we have developed a two-color flow cytometric erythrocyte binding assay (f-eba) that has several advantages over traditional erythrocyte binding assays (t-ebas) used in malaria research. we demonstrate the use of f-eba using the p. vivax duffy binding protein reg ... | 2005 | 15584018 |
| the clinical-grade 42-kilodalton fragment of merozoite surface protein 1 of plasmodium falciparum strain fvo expressed in escherichia coli protects aotus nancymai against challenge with homologous erythrocytic-stage parasites. | a 42-kda fragment from the c terminus of major merozoite surface protein 1 (msp1) is among the leading malaria vaccine candidates that target infection by asexual erythrocytic-stage malaria parasites. the msp1(42) gene fragment from the vietnam-oak knoll (fvo) strain of plasmodium falciparum was expressed as a soluble protein in escherichia coli and purified according to good manufacturing practices. this clinical-grade recombinant protein retained some important elements of correct structure, a ... | 2005 | 15618165 |
| comparative chromosome painting in aotus reveals a highly derived evolution. | the genus aotus represents a highly diverse group with an especially intricate taxonomy. no standard cytogenetic nomenclature for the genus has yet been established. so far, cytogenetic studies have characterized 18 different karyotypes with diploid numbers ranging from 46 to 58 chromosomes. by combining g-banding comparisons and molecular cytogenetic techniques, we were able to describe the most likely pattern of chromosome evolution and phylogenetic position of two aotus karyomorphs (kms) from ... | 2005 | 15645457 |
| the t-cell receptor in primates: identifying and sequencing new owl monkey trbv gene sub-groups. | the new world primate aotus nancymaae (owl monkey) has been shown to be an excellent experimental model when studying malarial parasites. characterising the t-cell receptor (tr) alphabeta repertoire by means of the different variable beta (trbv) genes displayed contributes to a better understanding of these lymphocytes' role in the response against several malarial antigens. this study describes identifying and characterising eleven new trbv gene sub-groups in cdna from aotus nancymaae's periphe ... | 2005 | 15711805 |
| structural and functional characterisation of the toll like receptor 9 of aotus nancymaae, a non-human primate model for malaria vaccine development. | in the absence of suitable rodent animal models for plasmodium falciparum malaria, the efficacy testing of asexual blood-stage vaccine candidates in aotus nancymaae represents a tool to select between different formulations before conducting expensive human clinical trials. cpg oligonucleotides (odn) specifically promote the production of pro-inflammatory and th1-type cytokines and they enhance the immunogenicity of co-administered antigens. toll like receptor 9 (tlr-9) binds directly and sequen ... | 2005 | 15900500 |
| studies on two strains of plasmodium cynomolgi in new world and old world monkeys and mosquitoes. | infections that cause the gombak and smithsonian strains of plasmodium cynomolgi were induced in macaca mulatta, aotus lemurinus griseimembra, aotus nancymai, and saimiri boliviensis monkeys. transmission of the gombak strain to aotus spp. monkeys was obtained by the injection of sporozoites dissected from the salivary glands of experimentally infected anopheles dirus and by the bites of infected an. dirus and anopheles farauti mosquitoes. two s. boliviensis monkeys were infected via the injecti ... | 2005 | 15986601 |
| observations on the vietnam palo alto strain of plasmodium vivax in two species of aotus monkeys. | thirty-three splenectomized aotus lemurinus griseimembra monkeys with no previous experience with malaria were infected with the vietnam palo alto strain of plasmodium vivax. the median maximum parasite count was 280,000/microl. nine splenectomized monkeys with previous infection with plasmodium falciparum had median maximum parasite counts of 120,000/microl. splenectomized aotus nancymai monkeys supported infections at a lower level. transmission via the bites of anopheles dirus mosquitoes was ... | 2005 | 15986626 |
| preliminary observations on the efficacy of a recombinant multistage plasmodium falciparum vaccine in aotus nancymai monkeys. | a vaccine trial was conducted to determine the efficacy of a multicomponent candidate vaccine, falvac-1, against plasmodium falciparum in aotus nancymai monkeys. after two immunizations, animals were challenged intravenously with parasites of the vietnam oak knoll (fvo) strain of p. falciparum. the primary outcome was to determine the protective response of the monkeys to immunization with the falvac-1 antigen produced in baculovirus when combined with different adjuvants (alum, qs-21, aso2a, cr ... | 2005 | 16222010 |
| identification of five different ighv gene families in owl monkeys (aotus nancymaae). | in order to characterize immunoglobulin heavy-chain variable (ighv) genes in aotus nancymaae monkeys, different mrnas encoded by five ighv families in this non-human primate were molecularly analysed considering their paramount importance in antibody production in an immune response. this study reports gene products exhibiting 91% amino acid similarity with ighv1, ighv2, ighv3, ighv4 and ighv7 human ighv families. our analyses suggest that the ighv gene has several conserved characteristics in h ... | 2005 | 16305680 |
| adaptation of plasmodium vivax to growth in owl monkeys (aotus nancymai). | the purpose of this study was reactivation and adaptation of a strain of plasmodium vivax to aotus nancymai monkeys. a need arose for malarial parasites for use in serologic and molecular studies and for teaching slides. this particular strain of parasite had been characterized previously as producing high-density parasitemia in splenectomized new world monkeys and therefore represented a good candidate for reactivation. p. vivax (vietnam ii), isolated in 1970, was reactivated after adaptation i ... | 2005 | 16422149 |
| immunization of aotus monkeys with recombinant cysteine-rich interdomain region 1 alpha protects against severe disease during plasmodium falciparum reinfection. | after continuous exposure to malarial infections in regions of africa where malaria is hyperendemic, children attain clinical immunity. this immunity results, in part, from the acquisition of antibodies against a large repertoire of variant antigens expressed on the surface of infected erythrocytes, such as the plasmodium falciparum erythrocyte membrane protein 1 (pfemp1). we determined whether a subunit vaccine to a portion of pfemp1 could induce protection in nonhuman primates. | 2006 | 16453270 |
| aotus nancymaae as a potential model for the testing of anti-sporozoite and liver stage vaccines against plasmodium falciparum. | the santa lucia strain of plasmodium falciparum was transmitted to aotus lemurinus griseimembra, a. azarae boliviensis, a. vociferans, and a. nancymaae monkeys by bite and by intravenous inoculation of sporozoites dissected from anopheles freeborni, an. stephensi, an. gambiae, an. albimanus, and an. maculatus mosquitoes. the data obtained from these infections indicate that a. nancymaae can be considered a suitable host model when combined with the santa lucia strain of p. falciparum for the tes ... | 2006 | 16525100 |
| social patterns of food sharing in monogamous owl monkeys. | captive owl monkeys (aotus nancymaae, a. azarai) share food frequently within both families and pairs. in this study food sharing was observed in seven mated pairs and four families (i.e., four mated pairs and their offspring). patterns of food sharing were examined with respect to age class, sex, and the presence or absence of dependent offspring. within families, most food transfers were from adult males to developing offspring. adult males and females transferred food to their mates in caged ... | 2006 | 16541439 |
| endemic infections of parastrongylus (=angiostrongylus) costaricensis in two species of nonhuman primates, raccoons, and an opossum from miami, florida. | parastrongylus (=angiostrongylus) costaricensis was first reported in the united states from cotton rats, sigmodon hispidus, in texas in 1979. here, we report the findings of p. costaricensis in a siamang (hylobates syndactylus) from the miami metrozoo, in 2 ma's night monkeys (aotus nancymaae) from the dumond conservancy located at monkey jungle in miami, in 4 raccoons (procyon lotor) trapped near the metrozoo, and in an opossum (didelphis virginiana) trapped at the metrozoo. these records are ... | 2006 | 16729706 |
| reference strand conformational analysis (rsca) is a valuable tool in identifying mhc-drb sequences in three species of aotus monkeys. | the aotus monkey has been of great value in the pre-clinical study of malaria vaccine candidates. several components of this primate's immune system have been studied and they display great similarity to their human counterparts. cloning and sequencing studies have revealed extensive sequence polymorphisms in aotus mhc-drb with very high similarities to several human allelic lineages, grouping at least nine distinct mhc-drb lineages. as the efficacy of peptide vaccines in this animal model may b ... | 2006 | 16733718 |
| owl monkey mhc-drb exon 2 reveals high similarity with several hla-drb lineages. | one hundred and ten novel mhc-drb gene exon 2 nucleotide sequences were sequenced in 96 monkeys from three owl monkey species (67 from aotus nancymaae, 30 from aotus nigriceps and 13 from aotus vociferans). owl monkeys, like humans, have high mhc-drb allele polymorphism, revealing a striking similarity with several human allele lineages in the peptide binding region and presenting major convergence with drb lineages from several catarrhini (humans, apes and old world monkeys) rather than with ot ... | 2006 | 16791623 |
| immunity to recombinant plasmodium falciparum merozoite surface protein 1 (msp1): protection in aotus nancymai monkeys strongly correlates with anti-msp1 antibody titer and in vitro parasite-inhibitory activity. | a number of malarial blood-stage candidate vaccines are currently being tested in human clinical trials, but our understanding of the relationship between clinical immunity and data obtained from in vitro assays remains inadequate. an in vitro assay which could reliably predict protective immunity in vivo would facilitate vaccine development. merozoite surface protein1 (msp1) is a leading blood-stage malaria vaccine candidate, and anti-msp1 antibodies from individuals that are clinically immune ... | 2006 | 16861644 |
| a linear peptide containing minimal t- and b-cell epitopes of plasmodium falciparum circumsporozoite protein elicits protection against transgenic sporozoite challenge. | an effective malaria vaccine is needed to address the public health tragedy resulting from the high levels of morbidity and mortality caused by plasmodium parasites. the first protective immune mechanism identified in the irradiated sporozoite vaccine, the "gold standard" for malaria preerythrocytic vaccines, was sporozoite-neutralizing antibody specific for the repeat region of the surface circumsporozoite (cs) protein. previous phase i studies demonstrated that a branched peptide containing mi ... | 2006 | 17030584 |
| studies on sporozoite-induced and chronic infections with plasmodium fragile in macaca mulatta and new world monkeys. | plasmodium fragile continues to be investigated because of its biologic similarities to the human malaria parasite, plasmodium falciparum. two strains of p. fragile are available for study; one strain is able to infect mosquitoes, whereas the other strain is transmissible only by blood inoculation. the sri lanka strain of p. fragile was transmitted to macaca mulatta, macaca fascicularis, aotus lemurinus griseimembra, aotus nancymaae, aotus vociferans, and saimiri boliviensis monkeys via sporozoi ... | 2006 | 17152944 |
| the antigenicity of a plasmodium vivax reticulocyte binding protein-1 (pvrbp1) recombinant fragment in humans and its immunogenicity and protection studies in aotus monkeys. | plasmodium vivax merozoites have been found to specifically invade immature red blood cells (reticulocytes) and this preference has been associated with two proteins named reticulocyte binding protein-1 and protein-2 (pvrbp1 and pvrbp2). previous reticulocyte binding assays using 15-mer synthetic peptides spanning the entire pvrbp1 sequence have shown that 25 out of the 195 peptides synthesised (grouped into 4 different regions) displayed high affinity binding to reticulocytes. the pvrbp1 region ... | 2007 | 17240494 |
| mutational changes in s-cone opsin genes common to both nocturnal and cathemeral aotus monkeys. | aotus is a platyrrhine primate that has been classically considered to be nocturnal. earlier research revealed that this animal lacks a color vision capacity because, unlike all other platyrrhine monkeys, aotus has a defect in the opsin gene that is required to produce short-wavelength sensitive (s) cone photopigment. consequently, aotus retains only a single type of cone photopigment. other mammals have since been found to show similar losses and it has often been speculated that such change is ... | 2007 | 17253622 |
| sex, age, and family differences in the chemical composition of owl monkey (aotus nancymaae) subcaudal scent secretions. | numerous behavioral studies have shown that animals use olfactory cues as inbreeding avoidance or kin avoidance mechanisms, implying that scent is unique to families. however, few studies have analyzed the chemical profile of a scent and ascertained the messages that are conveyed in scent secretions. owl monkeys (aotus nancymaae) are socially monogamous primates that utilize scent when interacting with foreign conspecifics. this suggests there is a difference in the chemical composition of scent ... | 2008 | 17577212 |
| flow cytometric analysis on cross-reactivity of human-specific cd monoclonal antibodies with splenocytes of aotus nancymaae, a non-human primate model for biomedical research. | the reactivity of 204 monoclonal antibodies (mab) out of 377 commercially available antibodies collected by the animal homologue group of the hlad8 was analyzed by single colour flow cytometry. most of these mab were originally developed against human cell surface molecules. fifty-eight mab (28%) showed reactivity with spleen cells of aotus nancymaae, a non-human primate animal model in biomedical research. out of these 58 mab, 22 also showed reactivity with mononuclear cells derived from rhesus ... | 2007 | 17644187 |
| ultrasonographic monitoring of a spontaneous abortion in an owl monkey (aotus nancymaae). | this case report describes the ultrasonographic findings during an idiopathic spontaneous abortion in an owl monkey. the female owl monkey presented for a transabdominal ultrasonogram to evaluate her pregnancy. this evaluation is a routine monitoring procedure in our owl monkey breeding colony. although the fetus and placenta appeared normal at the initial scan, no fetal heartbeat could be detected. we followed the abortion with serial ultrasonographic scans and documented complete involution of ... | 2007 | 17645301 |
| adaptation of a multi-drug resistant strain of plasmodium falciparum from peru to aotus lemurinus griseimembra, a. nancymaae, and a. vociferans monkeys. | a strain of plasmodium falciparum from peru was adapted to splenectomized aotus nancymaae and aotus vociferans monkeys. the peru 134/cdc strain of p. falciparum was shown to be resistant to treatment with chloroquine in monkeys and partially resistant to mefloquine and malarone. genetic mutations in crt, dhfr, dhps, and cytochrome b genes conferring drug resistance were also determined for this peruvian strain of p. falciparum. | 2007 | 17690397 |
| congenital radial and thumb aplasia in a neonatal owl monkey (aotus nancymaae). | this report describes congenital radial and thumb aplasia in a neonatal owl monkey. congenital limb deformities in human neonates and old world primate species have been well characterized. the many probable causes of these congenital defects in skeletal structure include fetal exposure to environmental toxins and genetic influences. in nonhuman primates, the cause frequently remains undetermined. in the case we present, the neonate presented for examination because of inability to cling to the ... | 2007 | 17877333 |
| erythrocyte binding protein pfrh5 polymorphisms determine species-specific pathways of plasmodium falciparum invasion. | some human malaria plasmodium falciparum parasites, but not others, also cause disease in aotus monkeys. to identify the basis for this variation, we crossed two clones that differ in aotus nancymaae virulence and mapped inherited traits of infectivity to erythrocyte invasion by linkage analysis. a major pathway of invasion was linked to polymorphisms in a putative erythrocyte binding protein, pfrh5, found in the apical region of merozoites. polymorphisms of pfrh5 from the a. nancymaae-virulent ... | 2008 | 18621009 |
| protection induced by plasmodium falciparum msp1(42) is strain-specific, antigen and adjuvant dependent, and correlates with antibody responses. | vaccination with plasmodium falciparum msp1(42)/complete freund's adjuvant (fa) followed by msp1(42)/incomplete fa is the only known regimen that protects aotus nancymaae monkeys against infection by erythrocytic stage malaria parasites. the role of adjuvant is not defined; however complete fa cannot be used in humans. in rodent models, immunity is strain-specific. we vaccinated aotus monkeys with the fvo or 3d7 alleles of msp1(42) expressed in escherichia coli or with the fvo allele expressed i ... | 2008 | 18665258 |
| [characterizing the cd3 epsilon chain from the new world primate aotus nancymaae]. | the t-cell receptor (tcr)-associated complex, cd3 (d, g, e) and z-chains are essential transmembrane proteins for signal transduction during t cell activation and immune response, as well as during thymocyte development. | 2008 | 18719729 |
| transmission of different strains of plasmodium cynomolgi to aotus nancymaae monkeys and relapse. | forty-four splenectomized aotus nancymaae monkeys were infected with 6 different strains of plasmodium cynomolgi, 11 via trophozoites and 33 via sporozoites. sporozoites from anopheles dirus, anopheles freeborni, anopheles gambiae, anopheles maculatus, and anopheles stephensi resulted in prepatent periods ranging from 9 to 39 days (median of 15 days). importantly, relapse was demonstrated in 5 of 5 sporozoite-induced infections with the rossan strain following treatment with chloroquine. | 2009 | 18788885 |
| characterization of a protective escherichia coli-expressed plasmodium falciparum merozoite surface protein 3 indicates a non-linear, multi-domain structure. | immunization with a recombinant yeast-expressed plasmodium falciparum merozoite surface protein 3 (msp3) protected aotus nancymai monkeys against a virulent challenge infection. unfortunately, the production process for this yeast-expressed material was not optimal for human trials. in an effort to produce a recombinant msp3 protein in a scaleable manner, we expressed and purified near-full-length msp3 in escherichia coli (ecmsp3). purified ecmsp3 formed non-globular dimers as determined by anal ... | 2009 | 19073223 |
| capsule polysaccharide conjugate vaccine against diarrheal disease caused by campylobacter jejuni. | the capsule polysaccharide (cps) of campylobacter jejuni is one of the few identified virulence determinants of this important human pathogen. since cps conjugate vaccines have been so effective against other mucosal pathogens, we evaluated this approach using cpss from two strains of c. jejuni, 81-176 (hs23 and hs36 serotype complex) and cg8486 (hs4 serotype complex). the cpss of 81-176 and cg8486 were independently linked to the carrier protein crm(197) by reductive amination between an aldehy ... | 2009 | 19114545 |
| susceptibility of the aotus nancymaae owl monkey to eastern equine encephalitis. | eastern equine encephalitis virus (eeev) is an arthropod-borne virus associated with life-threatening encephalitis in humans, equines, birds and many other domestic animals. to investigate the suitability of the aotus nancymaae new world owl monkey as a viable animal model for eee candidate vaccine testing we used clinical presentation, serology, viral isolation and pcr to evaluate pathogenesis and immunity in infected animals. monkeys were inoculated subcutaneously (sq) or intranasally (in) wit ... | 2009 | 19186197 |
| molecular cloning, sequence characterization and tissue transcription profile analyses of two novel genes: lck and cdk2 from the black-boned sheep (ovis aries). | the complete coding sequences of two sheep genes--lck and cdk2--were amplified using the rapid amplification of cdna ends method based on three sheep est sequences whose translated amino acids contain the domain ptkc_lck_bik and s_tkc domain, respectively. the sequence analyses of these two genes revealed that the sheep lck gene encodes a protein of 509 amino acids which has high homology with the lymphocyte-specific protein tyrosine kinase (lck) of eight species: bovine (99%), human (96%), dog ... | 2010 | 19340603 |
| a new world primate deficient in tetherin-mediated restriction of human immunodeficiency virus type 1. | human immunodeficiency virus type 1 (hiv-1) does not replicate in primary cells of new world primates. to better understand this restriction, we expressed owl monkey (aotus nancymaae) cd4 and cxcr4 in the owl monkey kidney cell line, omk. an hiv-1 variant modified to evade the owl monkey restriction factor trim-cyp replicated efficiently in these cells but could not replicate in primary a. nancymaae cd4-positive t cells. to understand this difference, we examined apobec3g and tetherin orthologs ... | 2009 | 19553332 |
| restasis for the treatment of 'dry eye' in aotus nancymaae. | this case report describes the treatment of three male owl monkeys (aotus nancymaae) diagnosed with chronic dry eye with a topical cyclosporine product, restasis, approved for use in humans. these owl monkeys had ocular disease resulting from procedures performed at a biotechnology company. they were moved to the center for neotropical primate research and resources at university of south alabama to be incorporated into the breeding colony. | 2009 | 19619131 |
| susceptibility of owl monkeys (aotus nancymaae) to experimental infection with bartonella bacilliformis. | bartonellosis, caused by bartonella bacilliformis, is a clinically significant disease in parts of south america, where it is characterized by fever and hemolytic anemia during the often-fatal acute stage and warty skin eruptions during chronic disease. in this study, we evaluated owl monkeys (aotus nancymaae) as a potential model for studying the immunogenicity and pathology of bartonellosis. two groups of animals (n = 3 per group) received either 9.5 x 10(7) cfu b. bacilliformis by the id rout ... | 2008 | 19793460 |
| plasmodium vivax dbp binding to aotus nancymaae erythrocytes is duffy antigen dependent. | plasmodium vivax is the second leading cause of malaria worldwide. invasion of human erythrocytes by p. vivax merozoites is dependent upon the interaction between the parasite duffy binding protein (pvdbp) and the erythrocyte duffy antigen receptor. therefore, disruption of this vital interaction is an attractive target for therapeutic intervention. although aotus nancymaae is a commonly used primate model for human p. vivax infections, it has not been confirmed that the interaction between ao. ... | 2010 | 19799492 |
| insect-foraging in captive owl monkeys (aotus nancymaae). | whereas the diets of diurnal primate species vary greatly, almost all nocturnal primate species consume insects. insect-foraging has been described in nocturnal prosimians but has not been investigated in owl monkeys (aotus spp.). we studied 35 captive owl monkeys (aotus nancymaae) in order to describe their foraging behavior and to determine if there were any age or sex differences in their ability to capture insect prey. because owl monkeys cooperate in parental care and in food-sharing, we ex ... | 2010 | 20523055 |
| mated pairs of owl monkeys (aotus nancymaae) exhibit sex differences in response to unfamiliar male and female conspecifics. | in socially monogamous species, mate-guarding could be a reproductive strategy that benefits both males and females, especially when males contribute to parental care. by actively guarding mates, males may reduce their chances of being cuckolded, whereas females that mate-guard may reduce the likelihood that their mates will desert them or acquire additional mates, and hence limit or reduce paternal care of offspring. owl monkeys (aotus spp.) are socially monogamous with biparental care of young ... | 2010 | 20623504 |
| identification, classification and evolution of owl monkeys (aotus, illiger 1811). | owl monkeys, belonging to the genus aotus, have been extensively used as animal models in biomedical research but few reports have focused on the taxonomy and phylogeography of this genus. moreover, the morphological similarity of several aotus species has led to frequent misidentifications, mainly at the boundaries of their distribution. in this study, sequence data from five mitochondrial regions and the nuclear, y-linked, sry gene were used for species identification and phylogenetic reconstr ... | 2010 | 20704725 |
| measurement of fetal biparietal diameter in owl monkeys (aotus nancymaae). | owl monkeys are new world primates frequently used in biomedical research. despite the historical difficulty of breeding owl monkeys in captivity, several productive owl monkey breeding colonies exist currently. the animals in the colony we describe here are not timed-pregnant, and determination of gestational age is an important factor in prenatal care. gestational age of human fetuses is often determined by using transabdominal measurements of fetal biparietal diameter. the purpose of this stu ... | 2010 | 20858355 |
| pattern of maternal circulating crh in laboratory-housed squirrel and owl monkeys. | the anthropoid primate placenta appears to be unique in producing corticotropin-releasing hormone (crh). placental crh is involved in an endocrine circuit key to the production of estrogens during pregnancy. crh induces cortisol production by the maternal and fetal adrenal glands, leading to further placental crh production. crh also stimulates the fetal adrenal glands to produce dehydroepiandrostendione sulfate (dheas), which the placenta converts into estrogens. there are at least two patterns ... | 2010 | 20872786 |
| characterisation and comparative analysis of mhc-dpa1 exon 2 in the owl monkey (aotus nancymaae). | the aotus nancymaae (owl monkey) is an important animal model in biomedical research, particularly for the preclinical evaluation of vaccine candidates against plasmodium falciparum and plasmodium vivax, which require a precisely typed major histocompatibility complex. the exon 2 from a. nancymaae mhc-dpa1 gene was characterised in order to infer its allelic diversity and evolutionary history. aona-dpa1 shows no polymorphism and is related to other primate dpa alleles (including catarrhini and p ... | 2010 | 20884341 |
| immunogenicity and protective efficacy of a psoralen-inactivated dengue-1 virus vaccine candidate in aotus nancymaae monkeys. | psoralens are photoreactive compounds that cross-link pyrimidines after exposure to uva radiation. in this experiment, we tested the protective efficacy of a psoralen-inactivated dengue vaccine candidate in non-human primates. two groups of 7 aotus nancymaae monkeys received either 10ng per dose of inactivated denv1 plus alum adjuvant or alum alone (controls). doses were injected intradermally on days 0, 14, and 28. monkeys then received a challenge inoculation of 1.1 × 10(4)pfus of westpac 74 d ... | 2011 | 21303709 |
| increased positive selection pressure within the complementarity determining regions of the t-cell receptor ß gene in new world monkeys. | because of the long-term co-evolution of tcr and mhc molecules, numerous nucleotide substitutions have accumulated within the domains of tcrß genes. we previously found that nonsynonymous nucleotide substitutions occurred more frequently in complementarity determining region (cdr)ß than in cdra, even though only a limited number of common marmoset (callithrix jacchus) and human t-cell receptor ß variable (trbv) sequences were compared. this interesting finding raised the question of whether the ... | 2011 | 21769905 |
| development of an aotus nancymaae model for shigella vaccine immunogenicity and efficacy studies. | several animal models exist to evaluate the immunogenicity and protective efficacy of candidate shigella vaccines. the two most widely used nonprimate models for vaccine development include a murine pulmonary challenge model and a guinea pig keratoconjunctivitis model. nonhuman primate models exhibit clinical features and gross and microscopic colonic lesions that mimic those induced in human shigellosis. challenge models for enterotoxigenic escherichia coli (etec) and campylobacter spp. have be ... | 2014 | 24595138 |
| immunogenicity of a chimeric plasmodium falciparum merozoite surface protein vaccine in aotus monkeys. | the production of properly folded, recombinant sub-unit plasmodium falciparum malaria vaccine candidates in sufficient quantities is often a challenge. success in vaccine immunogenicity studies in small animal models does not always predict immunogenicity in non-human primates and/or human subjects. the aim of this study was to assess the immunogenicity of a chimeric blood-stage malaria vaccine in aotus monkeys. this vaccine candidate includes the neutralizing b cell epitopes of p. falciparum me ... | 2016 | 26975721 |
| a replicating adenovirus capsid display recombinant elicits antibodies against plasmodium falciparum sporozoites in aotus nancymaae monkeys. | decades of success with live adenovirus vaccines suggest that replication-competent recombinant adenoviruses (rads) could serve as effective vectors for immunization against other pathogens. to explore the potential of a live rad vaccine against malaria, we prepared a viable adenovirus 5 (ad5) recombinant that displays a b-cell epitope from the circumsporozoite protein (csp) of plasmodium falciparum on the virion surface. the recombinant induced p. falciparum sporozoite-neutralizing antibodies i ... | 2014 | 25368113 |
| protective efficacy of a plasmodium vivax circumsporozoite protein-based vaccine in aotus nancymaae is associated with antibodies to the repeat region. | we have previously reported that vivax malaria protein 001 (vmp001), a vaccine candidate based on the circumsporozoite protein of plasmodium vivax, is immunogenic in mice and rhesus monkeys in the presence of various adjuvants. in the present study, we evaluated the immunogenicity and efficacy of vmp001 formulated with a tlr9 agonist in a water-in-oil emulsion. following immunization, the vaccine efficacy was assessed by challenging aotus nancymaae monkeys with p. vivax sporozoites. monkeys from ... | 2014 | 25329054 |