| seroprevalence of astrovirus types 1 and 6 in london, determined using recombinant virus antigen. | we have developed a microimmunofluorescence test (if) which uses cells infected with a recombinant baculovirus which expresses the capsid proteins of astrovirus types 1 or 6. the if test was sensitive and specific and the results for human astrovirus type 1 (hast-1) were comparable to those obtained by immune electronmicroscopy and radioimmunoassay. application of the test to a panel of 273 sera collected from patients and staff at two childrens hospitals in london showed that over 50% of the po ... | 1996 | 8760964 |
| genomic characterization of human astrovirus type 6 katano virus and the establishment of a rapid and effective reverse transcription-polymerase chain reaction to detect all serotypes of human astrovirus. | we previously reported that human astrovirus type 6 (hastv t6) was the etiologic agent of a large-scale outbreak of acute gastroenteritis that occurred in 1991 in katano city, osaka, japan [oishi et al., 1994]. the two representative strains, katano virus k23 and k24, have been analyzed by sequencing the open reading frame 2 (orf2) region after amplification by reverse transcription-polymerase chain reaction (rt-pcr). the orf2 region of hastv t6 strains, including k23, was found to be about 20 b ... | 2000 | 10745244 |
| one-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants in beijing, china and associated health risk assessment. | to evaluate the presence and distribution of the three main viruses (rotavirus, astrovirus, and norovirus) responsible for human acute gastroenteritis in sewerage system an one-year study was carried out in beijing, china. a total of 96 samples of influent and effluents from three sewage treatment plants (stps) were collected from november 2006 to october 2007. silica was used to concentrate viral particles from water samples and a reverse transcriptase-nested polymerase chain reaction (rt-neste ... | 2011 | 21245573 |
| one-year monthly survey of rotavirus, astrovirus and norovirus in three sewage treatment plants (stps) in beijing, china and associated health risk assessment. | to evaluate the presence and distribution of the three main viruses (rotavirus, astrovirus, and norovirus) responsible for human acute gastroenteritis in sewerage systems, a one-year study was carried out in beijing, china. a total of 96 samples of influent and effluents from three sewage treatment plants (stps) were collected from november 2006 to october 2007. silica was used to concentrate viral particles from water samples and a reverse transcriptase-nested polymerase chain reaction (rt-nest ... | 2011 | 22214071 |