| potato mop-top virus in infected cells. | | 1972 | 4113729 |
| expression of e6/e7 or sv40 large t antigen-coding oncogenes in human corneal endothelial cells indicates regulated high-proliferative capacity. | human corneal endothelial cells are thought to have limited capacity for proliferation. little is known about the mechanisms that regulate the proliferation of these cells. the authors introduced oncogenes into human corneal endothelial cells to modulate proliferation. in addition, they sought to establish cell lines to facilitate study of human corneal endothelial cells. | 1995 | 7822156 |
| conservation of coat protein sequence among isolates of potato mop-top virus from scotland and peru. | coat protein gene sequences of eight isolates of potato mop-top virus from the peruvian andes and of three isolates from scotland were compared. despite wide geographical separation, there was little sequence variation among all isolates. | 1996 | 8712928 |
| identification of structural similarities between putative transmission proteins of polymyxa and spongospora transmitted bymoviruses and furoviruses. | comparison of amino acid sequence and hydropathy profiles shows conserved, structural similarities between the capsid readthrough protein of potato mop top virus (transmitted by spongospora subterranea) and furovirus and bymovirus proteins implicated in transmission by polymyxa spp. this suggests that these proteins have a common ancestry and are involved in a common biological process: virus transmission by plasmodiophorid fungi. | 1996 | 8879126 |
| detection of potato mop-top virus capsid readthrough protein in virus particles. | potato mop-top furovirus (pmtv) rna 3 encodes the 20 kda coat protein and a larger readthrough protein of 67 kda. the readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. a 21 kda c-terminal fragment of the readthrough protein was doned, fused to glutathione s-transferase and expressed in e. coli. an antiserum prepared against purified fusion protein was used in elisa to detect the readthrough protein in extracts of pmtv-infected leaves. ... | 1997 | 9225055 |
| transfer rna mimicry in a new group of positive-strand rna plant viruses, the furoviruses: differential aminoacylation between the rna components of one genome. | recent sequencing of the genomes of several furoviruses--fungus-transmitted rod-shaped positive-strand plant viruses--has suggested the presence of trna-like structures (tlss) at the 3' ends of the genomic rnas. we show here that the genomic rnas of soil-borne wheat mosaic virus (sbwmv), beet soil-borne virus (bsbv), potato mop-top virus (pmtv), peanut clump virus (pcv), and indian peanut clump virus (ipcv) all possess functional tlss that are capable of high-efficiency valylation. while the sbw ... | 1998 | 9657004 |
| complete sequence of rna 1 and the presence of trna-like structures in all rnas of potato mop-top virus, genus pomovirus. | the complete nucleotide sequence (6043 nt) of rna 1 from potato mop-top virus (pmtv-sw), the type member of the genus pomovirus, was determined. the first (5'-terminal) open reading frame (orf 1) encodes a predicted protein of 148 kda. orf 2 extends through the opal stop codon of orf 1 producing a predicted readthrough protein of 206 kda which resembles the rna-dependent rna polymerases (rdrp) of other fungal-transmitted viruses. it includes a methyltransferase, a helicase and a gdd rdrp motif, ... | 1999 | 10573175 |
| potato mop-top virus: the coat protein-encoding rna and the gene for cysteine-rich protein are dispensable for systemic virus movement in nicotiana benthamiana. | full-length genomic cdna clones of the swedish isolate of potato mop-top virus (pmtv) were transcribed in vitro using t7 rna polymerase. the combination of rna 1, 2 and 3 synthesized in the presence of m(7)gpppg cap analogue was infectious when inoculated onto nicotiana benthamiana plants. also, the combination of rna 1 (encodes the viral replicase) with rna 3 [encodes the triple gene block proteins and a small cysteine-rich protein (crp)] was infectious and both rnas moved systemically in n. be ... | 2003 | 12655103 |
| nucleotide sequences of coat protein coding regions of six potato mop-top virus isolates. | coat protein (cp) coding regions of six potato mop-top virus (pmtv) isolates from the czech republic and denmark (54-10, 54-11, 54-15, 54-19, korneta and pacov) were sequenced. comparison of the obtained nucleotide sequences as well as alignment of the deduced amino acid sequences were performed. the obtained results showed that the isolates from different parts of europe seem to have highly conserved coding regions which is unexpected for a viral rna genome known for its high mutation rate. thu ... | 2003 | 12828342 |
| dna microarray: parallel detection of potato viruses. | dna microarray assay has become a useful tool for gene expression studies. less frequent is its application to detection of viruses or diagnostics of virus diseases. here we show design of a microscope slide-based microarray assay for simultaneous identification of several potato viruses. different primer pairs were designed or adopted to obtain specific amplicons from six potato viruses: potato virus a (pva), potato virus s (pvs), potato virus x (pvx), potato virus y (pvy), potato mop-top virus ... | 2003 | 12828343 |
| efficient bacterial expression of recombinant potato mop-top virus non-structural triple gene block protein 1 modified by progressive deletion of its n-terminus. | to obtain strong bacterial expression of proteins that seem to be hard to express in bacteria or are highly toxic for bacteria, it is possible to create a palette of similar constructs, differing only by several nucleotides, gradually deleted from the full-length clone by exonuclease iii. when a construct is equipped with the 6xhis tag, a simple colony-blot procedure can be performed and a colony giving strong and efficient expression can easily be selected for high range protein expression. we ... | 2005 | 15802230 |
| assessment of the integral membrane protein topology in living cells. | the bimolecular fluorescence complementation (bifc) phenomenon has been successfully applied for in vivo protein-protein interaction studies and protein tagging analysis. here we report a novel bifc-based technique for investigation of integral membrane protein topology in living plant cells. this technique relies on the formation of a fluorescent complex between a non-fluorescent fragment of the yellow fluorescent protein (yfp) targeted into a specific cellular compartment and a counterpart fra ... | 2006 | 16553902 |
| [incidence and altitudinal distribution of 13 virus cultures in solanum tuberosum (solanaceae) from costa rica]. | a survey was conducted in 30 fields located at three different altitudes in cartago, costa rica's main potato producing area. twenty plants were sampled per farm, for a total of 600 samples with 200 samples per altitude. elisa was used with commercial reagents to independently test for pvx, pvy, pvm, pva, pvs, plrv, pmtv, pamv, pvv, pvt, aplv, apmov and trsv. the presence of the following viruses was determined: pvx (77 %), pamv (62 %), plrv (42 %), trsv (42 %), pvt (39 %), pvv (37 %), pmtv (31% ... | 2006 | 18457151 |
| two plant-viral movement proteins traffic in the endocytic recycling pathway. | many plant viruses exploit a conserved group of proteins known as the triple gene block (tgb) for cell-to-cell movement. here, we investigated the interaction of two tgb proteins (tgb2 and tgb3) of potato mop-top virus (pmtv), with components of the secretory and endocytic pathways when expressed as n-terminal fusions to green fluorescent protein or monomeric red fluorescent protein (mrfp). our studies revealed that fluorophore-labeled tgb2 and tgb3 showed an early association with the endoplasm ... | 2004 | 15608333 |
| transient coexpression of individual genes encoded by the triple gene block of potato mop-top virus reveals requirements for tgbp1 trafficking. | tgbp1, tgbp2, and tgbp3, three plant virus movement proteins encoded by the "triple gene block" (tgb), may act in concert to facilitate cell-to-cell transport of viral rna genomes. transient expression of potato mop-top virus (genus pomovirus) movement proteins was used as a model to reconstruct interactions between tgb proteins. in bombarded epidermal cells of nicotiana benthamiana, green fluorescent protein (gfp)-tgbp1 was distributed uniformly. however, in the presence of tgbp2 and tgbp3, gfp ... | 2004 | 15305613 |
| extended sequence analysis of three danish potato mop-top virus (pmtv) isolates. | the entire nucleotide sequence for the coding regions of a danish pmtv isolate 54-15 was determined and compared to other known and sequenced isolates of pmtv. many nucleotide and amino acid changes were found in parts of rna coding for the triple gene block (tgb) proteins and in the part of the rna coding for the read-through region of the coat protein (cp). these regions for two other isolates, the mild one 54-10 and the severe one 54-19, were sequenced. only two amino acid changes were found ... | 2004 | 15284485 |
| [efficient approach for potato viral pathogen sensitive diagnostic and identification]. | potato, one of the most widespread agricultural plants in russia, is strongly affected by various pathogens of viral, bacterial, and fungal origin as well as by pests. their simple and accurate diagnostics and identification sound rather important both for production of virus free planting material and to perform monitoring of the phytosanitary state of planting areas. based on qualitative fluorescent amplification--based specific hybridization polymerase chain reaction (flash-pcr) we have devel ... | 2009 | 19548542 |
| production of recombinant potato mop-top virus coat protein in escherichia coli and generation of antisera recognising native virus protein. | potato mop-top virus (pmtv, pomovirus) is difficult to detect because it is unevenly distributed and present at low concentration in infected tissues. the production of pmtv-free seed relies on sensitive and specific detection methods of virus detection, including serological methods. the possibility of using a pmtv recombinant coat protein (cp) as an antigen for antiserum production was investigated. the region encoding the pmtv cp was inserted into pet3a, expressed in escherichia coli, and the ... | 2003 | 12757925 |
| the role of microtubule association in plasmodesmal targeting of potato mop-top virus movement protein tgbp1. | cell-to-cell movement of potato mop-top virus (pmtv) is mediated by three virus-encoded 'triple gene block' (tgb) proteins termed tgbp1, tgbp2 and tgbp3. tgbp1 binds virus rnas to form viral ribonucleoprotein complexes (vrnps), the transport form of viral genome. tgbp2 and tgbp3 are necessary for intracellular delivery of tgbp1-containing vrnps to plasmodesmata. to analyze subcellular localization and transport of tgbp1 we used a single binary vector for agrobacterium-mediated co-expression of p ... | 2011 | 21660184 |
| Resistance to multiple viruses in transgenic tobacco expressing fused, tandem repeat, virus-derived double-stranded RNAs. | Transgenic tobacco plants expressing fused, tandem, inverted-repeat, double-stranded RNAs derived either from the three viruses [potato virus Y (PVY), potato virus A (PVA), and potato leafroll virus (PLRV)] or the five viruses [PVY, PVA, PLRV as well as tobacco rattle virus (TRV), and potato mop-top virus (PMTV)] were generated in this study to examine whether resistance could be achieved against these three viruses or five viruses, respectively, in the same plant. The transgenic lines were engi ... | 2011 | 21853332 |
| identification of two nucleotide sequence sub-groups within potato mop-top virus. | to evaluate the variation of potato mop-top pomovirus from potato fields, 21 isolates were collected from different danish locations. reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (rt-pcr-rflp) of regions of rna2 was performed for all 21 isolates resulting in the establishment of two sub-groups of isolates. the nucleotide sequence of a region encoding part of the 'readthrough protein' of rna2 was compared for 9 of these isolates. this sequence analysis ... | 2003 | 12557000 |
| subcellular localisation, protein interactions, and rna binding of potato mop-top virus triple gene block proteins. | subcellular localisation, protein interactions, and rna binding of the triple gene block proteins (tgbp) of potato mop-top virus (pmtv) were studied. the 13-kda (tgbp2) and 21-kda (tgbp3) proteins with or without green fluorescent protein fused to their n-terminus, and the 51-kda protein (tgbp1) were expressed individually from a recombinant tobacco mosaic virus (tmv) vector. fluorescent images and western immunoblotting experiments of recombinant tmv-infected nicotiana benthamiana cells suggest ... | 2002 | 12093178 |
| [detection and molecular characterization of potato virus s (pvs, carlavirus) from colombia]. | in colombia, potato crops are affected by a wide variety of viruses such as pvy, plrv, pvx, pmtv and pvs. unfortunately, there are very few studies on the biology, distribution and pathogenicity of these viruses; this situation is even worse for the latent virus pvs. in this work, we evaluated the presence ofpvs in four colombian provinces (antioquia, boyacá, cundinamarca, nariño) by the use of elisa. we also studied the degree of molecular variation by sequence comparison of a segment of the ge ... | 2013 | 23885575 |
| initial infection of roots and leaves reveals different resistance phenotypes associated with coat protein gene-mediated resistance to potato mop-top virus. | resistance to the pomovirus potato mop-top virus (pmtv) was studied in potato (solanum tuberosum cv. saturna) and nicotiana benthamiana transformed with the coat protein (cp) gene of pmtv. the incidence of pmtv infections was reduced in tubers of the cp-transgenic potatoes grown in the field in soil infested with the viruliferous vector, spongospora subterranea. however, in those tubers that were infected, all three virus rnas were detected and virus titres were high. the cp-transgenic n. bentha ... | 2002 | 11961276 |
| the readthrough region of potato mop-top virus (pmtv) coat protein encoding rna, the second largest rna of pmtv genome, undergoes structural changes in naturally infected and experimentally inoculated plants. | molecular data on potato mop-top virus (pmtv), genus pomovirus, is currently mostly based on analysis of two scottish isolates, pmtv-s and pmtv-t. here we report the complete sequence of "the coat protein (cp) encoding rna" of an isolate of pmtv obtained from the field in sweden. our data show that this rna (3134 nt) is the second largest of the three rna species in the tripartite pmtv genome, and it should, therefore, be referred to as rna 2. this nomenclature is consistent with other pomovirus ... | 2001 | 11338384 |
| potato mop-top virus rna can move long distance in the absence of coat protein: evidence from resistant, transgenic plants. | transgenic expression of a translatable version of the potato mop-top virus (pmtv) coat protein (cp) gene (encoded by rna 3) in nicotiana benthamiana prevented production of symptoms and infective virus particles. rnas 1 and 2 accumulated in inoculated and systemic leaves but, apart from small amounts of cp transgene rna transcript, no genomic-length rna 3 was found. crude leaf extracts from inoculated plants were not infective. however, when rna extracts from such transgenic plants were inocula ... | 2000 | 10656594 |
| a naturally occurring deleted form of rna 2 of potato mop-top virus. | a spontaneous deletion in rna 2 of potato mop-top virus (pmtv) was identified by rt-pcr. the deletion occurred reproducibly during manual passage of two isolates of pmtv and during fungal inoculation of plants with viruliferous soil. the borders of the deletion were conserved in all instances and sequence analyses showed that a contiguous segment of 2113 nucleotides was deleted internally from the genomic rna 2, leaving the 5'- and 3'-terminal sequences. rt-pcr experiments also showed that the d ... | 1999 | 10466821 |
| diversity and evolution of potato mop-top virus. | nearly complete sequences of rna-cp and 3'-proximal rna-tgb were determined for 43 samples of potato mop-top virus (pmtv) originating from potato tubers and field soil from sweden, denmark and the usa. the results showed limited diversity and no strict geographical grouping, suggesting only a few original introductions of pmtv from the andes. two distinguishable types of rna-cp and rna-tgb were found in the samples, but no specific combination of them correlated with spraing symptoms in tubers. ... | 2015 | 25753427 |
| association of sequences in the coat protein/readthrough domain of potato mop-top virus with transmission by spongospora subterranea. | a monofungal culture of spongospora subterranea was unable to acquire and transmit the t isolate of potato mop-top pomovirus (pmtv-t), which has been maintained by manual transmission in the laboratory for 30 years. a recently obtained field isolate (pmtv-s) was efficiently acquired and transmitted by the same fungus culture. sequence analysis of the readthrough (rt) protein-coding region of pmtv-s showed the presence of an additional 543 nt in the 3' half of the coding region relative to that o ... | 1998 | 9780038 |
| the genome organization of the broad bean necrosis virus (bbnv). | the genome of the broad bean necrosis virus oita-isolate (bbnv-o) [rna1 (6.0 kb), rna2 (2.8 kb) and rna3 (2.4 kb)] was cloned and sequenced. computer analysis indicates that methyltransferase, helicase and rna-dependent rna polymerase (rdrp) motifs are present in rna1. the viral capsid protein (cp) cistron is located at the 5' terminal end of rna2 and the mr of cp (20 k) is close to that determined by sds-page analysis. an ochre codon (uaa) in the cp cistron is thought to be partially suppressed ... | 1998 | 9722878 |
| phylogenetic analysis of triple gene block viruses based on the tgb 1 homolog gene indicates a convergent evolution. | the complete nucleotide sequence of the triple gene block one (tgb 1) of cymbidium mosaic potexvirus (cymmv) was compared to those from other potex-, carla-, furo- and hordeiviruses. seven conserved motifs in the tgb 1, including the atp-gtp binding domain (p-loop) consensus gxxgktsts, were found in all four virus genera. we propose that all tgbv can be classified into phylogenetic clusters based on their tgb 1 homolog genes. these clusters can be further delineated to form subgroups. the first ... | 1998 | 9654683 |
| beet soil-borne virus rna 2: similarities and dissimilarities to the coat protein gene-carrying rnas of other furoviruses. | the complete sequence of the 3454 nt of rna 2 of the ahlum isolate of beet soil-borne furovirus (bsbv) has been determined starting with two short stretches of cloned cdna. unknown parts of the sequence were amplified by means of rt-pcr techniques using combinations of specific and random primers. bsbv rna 2 is more similar in its genetic organization to potato mop top virus (pmtv) rna 3 than to any other furoviral rna, although it is more than 1100 nt longer. its 3'-end, unlike that of pmtv rna ... | 1997 | 9018071 |
| beet soil-borne virus rna 3--a further example of the heterogeneity of the gene content of furovirus genomes and of triple gene block-carrying rnas. | the complete nucleotide sequence of rna 3 of the ahlum isolate of beet soil-borne virus (bsbv) was determined from cdnas obtained with immunocaptured virus particles and denatured preparations of dsrna. bsbv rna 3 is unique among the plant virus rnas studied so far in containing apparently only the coding sequences of a triple gene block (tgb). the derived amino acid sequences of the three putative tgb-encoded proteins showed the highest level of sequence similarities with those of the correspon ... | 1996 | 8614988 |
| the nucleotide sequence of potato mop-top virus rna 2: a novel type of genome organization for a furovirus. | particles of isolate t of potato mop-top furovirus (pmtv) contain three rna species (6.5, 3.0 and 2.5 kb). hybridization tests with cloned cdna probes showed that none of these species was derived from another. rna 2 (2962 nt), which was sequenced, has non-coding regions of 368 nt and 285 nt at the 5' end and 3' end, respectively. near the 5' terminus, nucleotides 46 to 110 are able to form a stem-loop structure, the stem of which has 23 bp with only one mismatch and one unpaired nucleotide. fro ... | 1994 | 7996148 |
| sequence analysis and gene content of potato mop-top virus rna 3: further evidence of heterogeneity in the genome organization of furoviruses. | the complete sequence of the 2315 nucleotides in rna 3 of potato mop-top furovirus (pmtv) isolate t was obtained by analysis of cdna clones and by direct rna sequencing. the sequence contains an open reading frame for the coat protein (20k) terminated by an amber codon, followed by an in-phase coding region for an additional 47k. pmtv therefore resembles soil-borne wheat mosaic (sbwmv) and beet necrotic yellow vein (bnyvv) viruses (two other fungus-transmitted viruses with rod-shaped particles) ... | 1995 | 7831829 |
| immunity to potato mop-top virus in nicotiana benthamiana plants expressing the coat protein gene is effective against fungal inoculation of the virus. | nicotiana benthamiana stem tissue was transformed with agrobacterium tumefaciens harboring a binary vector containing the potato mop-top virus (pmtv) coat protein (cp) gene. pmtv cp was expressed in large amounts in some of the primary transformants. the five transgenic lines which produced the most cp were selected for resistance testing. flowers on transformed plants were allowed to self-fertilize. transgenic seedlings selected from the t1 seed were mechanically inoculated with two strains of ... | 1995 | 7756694 |
| antigenic structure of the coat protein of potato mop-top furovirus. | the antigenic structure of the coat protein (cp) of potato mop-top furovirus (pmtv) was studied by electron microscopy of virus particles labeled with gold-conjugated monoclonal antibodies (mabs) and by the reactions of mabs with overlapping octapeptides (pepscan) representing the complete amino acid sequence of the cp. a total of seven epitopes were identified in the cp. mab scr 69 detected a continuous epitope, which was located at the extreme n-terminus of the cp, was exposed at the surface a ... | 1994 | 7519806 |
| unusual long-distance movement strategies of potato mop-top virus rnas in nicotiana benthamiana. | the potato mop-top virus (pmtv) genome encodes replicase, movement, and capsid proteins on three different rna species that are encapsidated within tubular rod-shaped particles. previously, we showed that the protein produced on translational readthrough (rt) of the coat protein (cp) gene, cp-rt, is associated with one extremity of the virus particles, and that the two rnas encoding replicase and movement proteins can move long distance in the absence of the third rna (rna-cp) that encodes the c ... | 2009 | 19271953 |
| the beet virus q coat protein readthrough domain is longer than previously reported, with two transmembrane domains. | ten beet virus q (bvq) strains from six different countries were sequenced to characterize the readthrough (rt) domain of the coat protein (cp). the genbank/embl/ddbj accession numbers for the sequences reported in this paper are fm244643-fm244652. with three nucleotide additions of 5, 285 and 1 nt, the common rt of 76 kda was found to be longer than the single reference available to date (35 kda). it is hypothesized that multiple inoculation cycles on chenopodium quinoa were responsible for the ... | 2009 | 19218222 |
| recent developments in design and application of plant virus-based gene vectors. | a new horizon is lit up by exploiting plant viruses as the vectors to deliver foreign genes into plants for various purposes such as production of valuable pharmaceutical proteins, to understand pathogenesis of a plant virus, and to establish gene silencing for blocking, for example, production of an undesirable intermediate metabolite in a metabolic pathway. here, the recent patents concerning the design of new gene vectors on the basis of the genomes of the viruses including tobacco mosaic vir ... | 2007 | 19075936 |
| detection of potato mop top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. | tobacco rattle virus (trv) and potato mop top virus (pmtv) are important diseases of potato that are difficult to diagnose reliably by visual symptoms. effective control strategies rely on accurate diagnosis. this paper describes the development of a multiplex assay for the detection of trv and pmtv directly from potato tubers and leaves by polymerase chain reaction (pcr) combined with in-tube fluorescent product detection (taqman). this technology obviates any post-pcr manipulations and has man ... | 2000 | 18944548 |
| sequence analysis of the czech potato mop-top virus (pmtv) isolate korneta-nemilkov. | the nucleotide sequence was determined for czech potato mop-top virus (pmtv) isolate korneta-nemilkov, found in the potato field situated in south bohemia. the nucleotide and amino acid sequences were compared with other pmtv isolates available in databases. the sequence identity was always >99% when czech isolate rna 2 and rna 3 sequences were compared with each of the 3 danish isolates and with sw isolate, and slightly lower when compared to scottish isolates. similarity of deduced proteins wa ... | 2007 | 17571797 |
| expression, localization and effects on virulence of the cysteine-rich 8 kda protein of potato mop-top virus. | potato mop-top virus (pmtv) rna3 contains a triple gene block (tgb) encoding viral movement proteins and an open reading frame for a putative 8 kda cysteine-rich protein (crp). in this study, pmtv crp was shown to be expressed in the course of virus infection, and a pmtv crp-specific subgenomic rna was mapped. crp has previously been shown to be dispensable for infection of pmtv in nicotiana benthamiana. in this study, pmtv crp was found to increase the severity of disease symptoms when expresse ... | 2005 | 16186244 |
| an accelerated soil bait assay for the detection of potato mop top virus in agricultural soil. | an accelerated soil bait test can be used to determine whether a field harbours virus-carrying spongospora subterranea. s. subterranea is the causal agent of powdery scab and also the only vector of potato mop top virus (pmtv). real-time rt-pcr can detect pmtv rna in the roots of bait plants after 2 weeks of growth in viruliferous soil. this test may be used to assess the risk of planting potato crops in a particular field. | 2009 | 19301761 |
| detection of potato mop-top virus in potato tubers and sprouts: combinations of rna2 and rna3 variants and incidence of symptomless infections. | potato mop-top virus (pmtv, genus pomovirus) causes severe quality problems by inducing necrotic arcs (spraing symptoms) in potato tubers. in this study, coat protein (cp) gene and read-through domain of rna2 and 8k gene and 3' untranslated region of rna3 were characterized from 37 pmtv isolates detected in tubers from fields in finland and a screenhouse in latvia. two distinguishable types of rna2 and rna3 were found, each showing only little genetic variability. sequencing and restriction frag ... | 2009 | 19351248 |
| the n-terminal domain of pmtv tgb1 movement protein is required for nucleolar localization, microtubule association, and long-distance movement. | the triple-gene-block (tgb)1 protein of potato mop-top virus (pmtv) was fused to fluorescent proteins and expressed in epidermal cells of nicotiana benthamiana under the control of the 35s promoter. tgb1 fluorescence was observed in the cytoplasm, nucleus, and nucleolus and occasionally associated with microtubules. when expressed from a modified virus (pmtv.yfp-tgb1) which formed local lesions but was not competent for systemic movement, yellow fluorescent protein (yfp)-tgb1 labeled plasmodesma ... | 2010 | 20923354 |
| the potato mop-top virus tgb2 protein and viral rna associate with chloroplasts and viral infection induces inclusions in the plastids. | the potato mop-top virus (pmtv) triple gene block 2 (tgb2) movement proteins fused to monomeric red fluorescent protein (mrfp-tgb2) was expressed under the control of the pmtv subgenomic promoter from a pmtv vector. the subcellular localizations and interactions of mrfp-tgb2 were investigated using confocal imaging [confocal laser-scanning microscope, (clsm)] and biochemical analysis. the results revealed associations with membranes of the endoplasmic reticulum (er), mobile granules, small round ... | 2012 | 23269927 |
| the cysteine-rich proteins of beet necrotic yellow vein virus and tobacco rattle virus contribute to efficient suppression of silencing in roots. | many plant viruses encode proteins that suppress rna silencing, but little is known about the activity of silencing suppressors in roots. this study examined differences in the silencing suppression activity of different viruses in leaves and roots of nicotiana benthamiana plants. infection by tobacco mosaic virus, potato virus y and cucumber mosaic virus but not potato virus x (pvx) resulted in strong silencing suppression activity of a transgene in both leaves and roots, whereas infection by b ... | 2012 | 22647371 |
| molecular and pathobiological characterization of sixty one potato mop-top virus full-length cdnas reveals great variability of the virus in the center of potato domestication, novel genotypes and evidence for recombination. | evolutionary divergence of potato mop-top virus (pmtv), a tri-partite single-stranded rna virus, is exceptionally low, based on the analysis of sequences obtained from isolates from europe, asia, and north america. in general, rna viruses exist as dynamic populations of closely related and recombinant genomes that are subjected to continuous genetic variation. the reason behind the low genetic variation of pmtv remains unclear. the question remains whether low variability is a shared property of ... | 2017 | 28390168 |
| molecular and biochemical examination of spraing disease in potato tuber in response to tobacco rattle virus infection. | field-grown tubers of potato were examined for infection by tobacco rattle virus (trv) and consequent production of corky ringspot or spraing symptoms. a microarray study identified genes that are differentially expressed in tuber tissue in response to trv infection and to spraing production, suggesting that hypersensitive response (hr) pathways are activated in spraing-symptomatic tubers. this was confirmed by quantitative reverse transcription-polymerase chain reaction (qrt-pcr) of a selected ... | 2016 | 27681277 |
| molecular and biological characterisation of two novel pomo-like viruses associated with potato (solanum tuberosum) fields in colombia. | potato is the fourth most important crop worldwide that is used as a staple food, after rice, wheat and maize. the crop can be affected by a large number of pathogens, including fungi, oomycetes, bacteria and viruses. diseases caused by viruses are among the most important factors contributing to reduced quality and yield of the crop. potato mop-top virus (genus pomovirus) induces necrotic flecks in the tuber flesh and skin of potato in temperate countries. spongospora subterranea is the vector ... | 2016 | 27016929 |
| elucidation of virus-host interactions to enhance resistance breeding for control of virus diseases in potato. | potato virus y (pvy) and potato mop-top virus (pmtv) are viruses whose geographical distribution is expanding and economic losses are increasing, in contrast to most of other viruses infecting potato crops. most potato cultivars lack broad-spectrum resistance to the new, genetically complex strains of pvy, and no efficient resistance to pmtv is known in potato. control of the vectors of these viruses is not an efficient or possible strategy to prevent infections. studies on molecular virus-host ... | 2015 | 25931981 |
| importin-α-mediated nucleolar localization of potato mop-top virus triple gene block1 (tgb1) protein facilitates virus systemic movement, whereas tgb1 self-interaction is required for cell-to-cell movement in nicotiana benthamiana. | recently, it has become evident that nucleolar passage of movement proteins occurs commonly in a number of plant rna viruses that replicate in the cytoplasm. systemic movement of potato mop-top virus (pmtv) involves two viral transport forms represented by a complex of viral rna and triple gene block1 (tgb1) movement protein and by polar virions that contain the minor coat protein and tgb1 attached to one extremity. the integrity of polar virions ensures the efficient movement of rna-cp, which e ... | 2015 | 25576325 |
| complete genomic characterization of a potato mop-top virus isolate from the united states. | potato mop-top virus (pmtv; family virgaviridae) was reported recently in the pacific northwestern usa. to better understand the genetic diversity of this virus, the complete genome of an isolate from washington state (wa), usa, was characterized. sequence comparisons of the wa isolate with other known sequences revealed that the rna-rep-encoded rdrp protein and the rna-cp-encoded coat protein displayed >99 % amino acid sequence identity to those of two nordic (rdrp) and several european and nor ... | 2014 | 25287129 |
| detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, elisa and rt-pcr. | the hilly region of northwest of pakistan is leading seed potato producing areas of the country. soil and plant samples were collected from the region and tested for pmtv using both conventional and molecular techniques. the bait plants exhibited pmtv-characteristic v-shaped yellow leaf markings in nicotiana debneyi plants grown in putative viruliferious soils from 20/26 locations. the results were confirmed by back inoculation of sap from both roots and leaves of bait plant on indicator hosts ( ... | 2014 | 24161813 |
| deciphering the mechanism of defective interfering rna (di rna) biogenesis reveals that a viral protein and the di rna act antagonistically in virus infection. | potato mop-top virus (pmtv) produces a defective rna (d rna) encompassing the 5'-terminal 479 nucleotides (nt) and 3'-terminal 372 nt of rna-tgb (where tgb is triple gene block). the mechanism that controls d rna biogenesis and the role of d rna in virus accumulation was investigated by introducing deletions, insertions, and point mutations into the sequences of the open reading frames (orfs) of tgb1 and the 8-kilodalton (8k) protein that were identified as required for efficient production of t ... | 2013 | 23514891 |
| tyrosine phosphorylation of the triple gene block protein 3 regulates cell-to-cell movement and protein interactions of potato mop-top virus. | functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases in plants, but little is known about the involvement of tyrosine kinases in plant virus infection. in this study, tgbp3, one of the three movement proteins encoded by a triple gene block (tgb) of potato mop-top virus (pmtv), was detected for the first time in pmtv-infected plants and found to be tyrosine phosphorylated. phosphorylation sites (tyr(87-89) and tyr(120)) were located in two amino acid mo ... | 2013 | 23365450 |