Publications

TitleAbstractYear
Filter
PMID
Filter
predominance of mite allergy over allergy to pollens and animal danders in a farming population.the prevalence of ige-mediated hypersensitivity to respiratory allergens, including lepidoglyphus destructor (a storage mite) and dermatophagoides pteronyssinus, was studied in a rural population of gotland, a swedish island in the baltic sea. a sample of 440 farmers underwent examination, comprising skin-prick tests (animal danders, pollens, moulds, house dust mite and wheat) and blood sampling for radio-allergosorbent tests (rast) (birch, timothy grass, dog, d. pteronyssinus, cladosporium and ...19873499998
sensitization to storage mites and other work-related and common allergens among finnish dairy farmers.skin-tests with the prick technique were made on 121 dairy farmers with allergic rhinitis and/or asthma (symptomatic subjects), 64 dairy farmers without respiratory symptoms (asymptomatic subjects), and 26 non-farming controls. the antigen panel consisted of the storage mites acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae, as well as other work-related allergens (cereal grains, animal epithelia, fungi and yeasts), and common allergens (house dust mite, pollens). mean areas o ...19873478215
storage mites in the work environment of farmers.occurrence of storage mites was studied in cow houses and hay stores on 19 farms, most of which were located in eastern finland. on two farms we did a monthly follow-up. mites were extracted from hay by sieving, using the so-called "water trap" and for longitudinal analysis, with a warm extractor. the mites isolated in the water trap were identified under a microscope. contrary to previous finnish findings, there apparently were more mites in cow houses than in hay stores. in cow houses there we ...19873478210
[allergy to tyrophagus putrescentiae and lepidoglyphus destructor in a population of young adult asthmatics].the goal of this work was the study of the prevalence of allergy to two mites, tyrophagus putrescentiae and lepidoglyphus destructor in a population of 105 young asthmatic men from east of france. the association of skin tests and specific ige shows that 43.10% are allergic to tyrophagus and 44.95% to lepidoglyphus destructor. the significance of skin tests is doubtful and depends on the laboratories which provide the allergens. therefore, specific ige has to be performed. as in other studies it ...19863453717
study of the sensitization to storage mites in a pediatric population in barcelona.we performed the following study in order to determine the prevalence and characteristics of sensitization to storage mites in children mostly from the city of barcelona. all children over 3 years of age attending our unit for the first time due to respiratory problems were given cutaneous tests using the prick test for the house dust mites dermatophagoides pteronyssinus and d. farinae, and the storage mites acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae. we carried out a ras ...19938281346
demonstration of allergen components in the storage mite lepidoglyphus destructor by an immunoblotting technique.the allergen composition of crude extracts of the storage mite lepidoglyphus destructor were investigated by sodium dodecylsulphate polyacrylamide gel electrophoresis and immunoblotting. the allergen components were detected by sera from allergic farmers with a positive radioallergosorbent test to l. destructor. the allergen-antibody complexes were visualized by rabbit anti-ige and beta-galactosidase-labelled sheep antirabbit igg in a chromogenic substrate. six out of the forty-three sera also h ...19883338856
clinical significance and allergenic cross-reactivity of euroglyphus maynei and other nonpyroglyphid and pyroglyphid mites.the clinical significance and allergenic cross-reactivity of the storage mites lepidoglyphus destructor and acarus siro and the house dust mites dermatophagoides pteronyssinus and euroglyphus maynei were investigated with specific ige antibodies by use of the rast and the rast-inhibition technique. rast-positive sera were obtained from 64 swedish farmers whose test results were positive to at least one of the four mite species. e. maynei was shown to be a common cause of sensitization among the ...19892926080
skin and serum reactivity to some storage mites in children sensitive to dermatophagoides pteronyssinus.the incidence of skin test and rast positivity for dermatophagoides farinae and storage mites (glycyphagus privatus, lepidoglyphus destructor, tyrophagus putrescentiae, acarus siro, and gohieria fusca) have been evaluated in children with bronchial asthma and positive prick test to dermatophagoides pteronyssinus. the storage mite with highest incidence of skin and rast positivity was gohieria fusca, which caused a positive response in more than 50% of the children evaluated. this antigen appears ...19892742217
[pathology caused by acari].this report concerns the observations the authors have been carrying out for over twelve years in sardinia; they regard the sensitization to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and to storage mites (acarus siro, glycyphagus domesticus, lepidoglyphus destructor, tyrophagus putrescentiae) and are been obtained with: 1) skin tests with mite extracts; 2) radioallergosorbenttest (rast) for specific ige and igg; 3) mite exposure in homes and mite analysis; 4) hy ...19892519754
dermatophagoides and storage mites: statistical analysis of rast results.r.i.a. dosages of specific ige antibodies to dermatophagoides pteronyssinus, dermatophagoides farinae (house dust mites), acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae (storage mites) in 467 unselected patients were statistically analyzed. a significant noncorrelation between specific ige antibodies to storage mites and those to house dust mites has been found, suggesting that storage mites have their own allergenic specificity.19902321810
ige antibodies to aeroallergens in allergic children in são paulo, brazil.the sensitization to inhaled allergens was studied in 80 brazilian children and 12 controls, aged 6 to 16 years. we performed skin tests with alternaria alternata, cat, dog, lolium perenne, grasses and the following domestic mites: blomia tropicalis, dermatophagoides pteronyssinus, dermatophagoides farinae, lepidoglyphus destructor, chortoglyphus arcutus and aleuroglyphus ovatus. the frequencies of positive skin tests (mean diameter > or = 23 mm) were respectively 15%, 11%, 11%, 6%, 7%, 95%, 92% ...19979330189
comparison of clinical significance and allergenic cross-reactivity of storage mites blomia kulagini and lepidoglyphus destructor in sweden and brazil.comparison of the clinical significance and allergenic cross-reactivity of blomia kulagini (b. kulagini) and lepidoglyphus destructor (l. destructor) was made on sera from sweden and brazil using the radio-allergo-sorbent test (rast) and the rast inhibition technique. rast-positive sera were obtained from 53 allergic swedish farmers and 31 allergic subjects from brazil who were positive to b. kulagini and/or l. destructor. b. kulagini was shown to be a common cause of sensitization especially in ...19902244671
storage mites dominate the fauna in swedish barn dust.the mite fauna in hay dust of 16 farms located on the island of gotland was analysed to establish whether there is a clear relationship between environmental exposure to storage mites and development of allergic symptoms. eight of these farms belonged to farmers with respiratory symptoms related to barn dust (rs) and eight to healthy farmers (h). all farmers had previously participated in an epidemiological survey of hypersensitivity to respiratory allergens. the symptomatic farmers had not only ...19912039080
sensitization to blomia tropicalis and lepidoglyphus destructor in dermatophagoides spp-allergic individuals.serum was collected from 77 atopic subjects from cartagena, colombia, who had a positive skin test (greater than or equal to 3 mm) to either dermatophagoides farinae (df) (73) or d. pteronyssinus (dp) (70). twenty-nine healthy volunteers with negative allergy histories and negative skin tests were used as control subjects. rast and rast-inhibition assays were used to study the prevalence of specific ige to blomia tropicalis (bt), lepidoglyphus destructor (ld), and df. rast results were considere ...19911744365
skin cross-reactivity between dermatophagoides pteronyssinus and storage mites in atopic children.in our environment, some children with allergic respiratory pathology do not have as favorable an evolution as expected after three years of immunotherapy with d. pteronyssinus extracts. we believe this is due to the existence of other mites whose antigenic determinants are not present in d. pteronyssinus. the purpose of the present study was to evaluate skin cross-reactivity between dermatophagoides and storage mites in children with bronchial asthma. fifty patients were chosen, with a mean age ...19911669575
comparison of allergic responses to dust mites in u.k. bakery workers and swedish farmers.the ige rast response to dermatophagoides pteronyssinus and four storage mites (lepidoglyphus destructor, tyrophagus putrescentiae, glycyphaghus domesticus, and acarus siro) was examined in 251 u.k. bakery workers and compared with that previously found in 440 swedish farmers. storage mites are found commonly in stored hay and grain so both these groups potentially encounter them in their work. in neither group of workers was a positive rast (greater than or equal to 0.35 pru) to d. pteronyssinu ...19921571817
localization of major allergens in the dust mite lepidoglyphus destructor with confocal laser scanning microscopy.the dust mite lepidoglyphus destructor is the dominating source of allergens giving rise to asthma and rhinitis among farmers. in a previous study of the localization of allergens in l. destructor we demonstrated that the 39 kda allergen is associated with digestion. here we describe the localization of the principal 15 kda allergen and the high molecular weight allergen complex (79 and 93 kda) in l. destructor with confocal laser scanning microscopy (clsm). cryostat-cut sections of mite bodies ...19957648460
food-induced and occupational asthma due to barley flour.occupational exposure to inhalant allergens may induce asthma but the presence of asthma after the ingestion of the allergen is rarely reported.19957648375
[skin tests for different species of acari in asthmatic children from mexico city].house dust mites, dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) are the predominant cause of allergic asthma in our population. to detect the presence of sensitivity to other mites besides dp and df in 100 children that live in mexico city, skin tests were performed in their arms with multi-test devices and glycerinated extracts. there were 34 female and 66 male, aged 4 to 14 years (x = 9.1y), with history of mild and moderate asthma, 2 months to 12 years of evolution. dp ...19957552183
localization of allergens in the domestic mite lepidoglyphus destructor.the non-pyroglyphid domestic mite lepidoglyphus destructor is a major source of allergen causing respiratory symptoms in farming environments. this study is the first to focus on the localization of the allergens in the non-pyroglyphid domestic mite lepidoglyphus destructor. cryostat-cut sections of l. destructor mite bodies and faecal pellets were probed with one of three mouse monoclonal antibodies (moabs) raised against l. destructor or with patient sera, and stained with immunoperoxidase. ei ...19921373986
sensitization in a grain handler to the storage mite lepidoglyphus destructor (schrank).allergy to one of the most common canadian storage mites. lepidoglyphus destructor, was demonstrated in a grain worker by immediate skin testing and inhalation challenge. the frequency of sensitization to this mite was found to be 12% in 100 asthmatics by skin testing. in 4% of these asthmatics sensitization to another storage mite, acarus siro, was found, all of whom were also sensitized to l. destructor and the house dust mite, dermatophagoides farinae. there was a significantly higher proport ...19836849517
storage mite allergy is common in a farming population.the prevalence of storage mite allergy was investigated in an epidemiological study of respiratory symptoms in farmers on gotland, an island in the baltic sea. a questionnaire concerning work-related and chronic symptoms from the airways and eyes was completed by 2578 farmers. a sample of 440 farmers subsequently underwent examination comprising skin-prick tests (animal danders, pollens, moulds and house dust mite), blood sampling for rast against four storage mites (acarus siro, lepidoglyphus d ...19854075515
storage mites as a cause of bronchial asthma in denmark.from october 1982 to july 1983, 133 consecutive patients complaining of allergic symptoms were prick-tested with three storage mites (acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae) and two housedust mites (dermatophagoides pteronyssinus and dermatophagoides farinae). a total of 60 patients (45%) had a positive prick-test for one or more of the storage mites. a mutual positive correlation was found between the prick-test results for the two house-dust mites as well as for the th ...19854003230
allergy to storage mites or cow dander as a cause of rhinitis among finnish dairy farmers.the role of storage mites acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae, compared with that of cow dander, as a cause of allergic rhinitis was studied by nasal challenges in a population of 106 non-smoking dairy farmers. thirty-six of the subjects were challenged with storage mites alone, each with only one of the three species in consecutive order, 43 with cow dander alone, and 27 with both. thus mite challenges were performed on 63 farmers and cow dander challenges on 70. ...19853977027
[acari of food storage facilities. an ecologic and immuno-allergic study].the authors studied the commonest species of storage mites, living in granaries and in house dust. they found nine species of mites belonging to different systematic groups and were identified: acarus siro, tyrophagus putrescentiae, glycyphagus domesticus, lepidoglyphus destructor, chortoglyphus arcuatus, androlaelaps casalis, cheyletus fortis, cheyletus tenuipilis, thyreophagus entomophagus. skin tests were performed on 14 patients with house dust allergy, using extracts of three storage mites, ...19853837918
detection of at least one high-molecular-mass, ige-binding component of the dust mite lepidoglyphus destructor.we studied the allergen composition in an extract of the storage mite lepidoglyphus destructor. monoclonal antibodies (mabs) were raised against l. destructor by a standard hybridoma technique. in the subsequent screening, we concentrated on mabs fulfilling three criteria. first, in elisa, mabs were assessed against a panel of various mite species, and only those reacting exclusively with l. destructor extract were selected for further analyses. secondly, mabs were selected in immunoblotting acc ...19947653740
a naturally occurring model of immunoglobulin e antibody-mediated hypersensitivity in laboratory animals.a naturally occurring model of immunoglobulin e (ige) antibody-mediated hypersensitivity in canine and rhesus monkey species to environmental allergens is described. cutaneous responses to a dust mite mix (dermatophagoides farinae/pteronyssinus) and to four storage mite extracts (aleuroglyphus ovatus, blomia tropicalis, chortoglyphus sp., lepidoglyphus destructor) occurred in the canine species. rhesus monkeys had ige antibody-mediated hypersensitivity to dust mite on cutaneous challenge, but to ...19948201269
mite allergy in the tropics: sensitization to six domestic mite species in cartagena, colombia.sensitization to the domestic mites blomia tropicalis, chortoglyphus arcuatus, lepidoglyphus destructor and aleuroglyphus ovatus was investigated in 77 subjects with clinical symptoms of asthma and/or allergic rhinitis and a positive skin prick test (wheal > or = 3 mm) to dermatophagoides pteronyssinus and/or d. farinae. seventy patients were skin test-positive to d. pteronyssinus and 73 to d. farinae. serum samples from 29 nonallergic, skin test-negative individuals were used as negative contro ...19938281353
inhaled aeroallergen and storage mite reactivity in a wisconsin farmer nested case-control study.a nested case-control study was undertaken to assess the role of antibody (ab) to a panel of antigens associated with hypersensitivity pneumonitis in the development of lung disease among wisconsin dairy farmers. immunologic reactivity to a panel of inhaled allergens was assessed in the ab+ cases and compared to the ab+ control, ab- control, and ab- blue-collar control subjects by skin tests, rast assays, and a modified american thoracic society questionnaire. the most prevalent allergens among ...19938430958
crossreactivity between dermatophagoides siboney and other house dust mite allergens in sensitized asthmatic patients.the role of pyroglyphid mites in ige-mediated sensitization has been well established. although mites belonging to the genus dermatophagoides dominate in the acarofauna of domestic dust, non-pyroglyphid mites might also be of clinical importance. in cuba, dermatophagoides siboney is found in dust coexisting with d. pteronyssinus and blomia tropicalis. dermatophagoides farinae is not found. storage mites, such as acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae, might also be pr ...19958556563
allergen-specific ige levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects.sensitization to allergens has been shown to be a risk factor for adults with acute asthma first seen in the emergency department.19968757201
prevalence of specific ige to storage mites (a. siro, l. destructor and t. longior) in an urban population and crossreactivity with the house dust mite (d. pteronyssinus).the role of pyroglyphid mites in house dust allergy is well established and the major allergens from the common house dust mites (dermatophagoides species) have been characterized. there is, however, relatively little progress in the understanding of the human ige response to non-pyroglyphid storage mites, allergenic crossreactivity with other mite species and extent of environmental exposure. we studied 196 individuals from an urban environment who were not occupationally exposed to storage mit ...19901695869
aeroallergens in dairy barns near cooperstown, new york and rochester, minnesota.we sampled atmospheric barn air using a volumetric air sampler in ten barns near cooperstown, ny and six barns near rochester, mn, and, with radioimmunoassays, measured allergens of aspergillus fumigatus, thermoactinomyces vulgaris, micropolyspori faeni, short ragweed, rye grass group i pollen, alternaria (alt-1), dermatophagoides sp. lepidoglyphus destructor, common insect allergen, mouse urine, rat urine, and cattle epithelium. the most abundant allergen present was a. fumigatus followed by l. ...19892764367
occupational allergic disease in cereal workers by stored grain pests.it is well known that workers occupationally exposed to grain dust have a high prevalence of respiratory symptoms, but their pathogenesis remains obscure when sensitization to cereal flour cannot be demonstrated. storage mites, tenebroids, and cockroaches are stored-grain pests found in grain and cereal products frequently in our area, where the cereal industry is the most important industry. an epidemiological analysis of sensitization of these stored-grain pests was performed on 4379 patients ...19979350153
mite fauna of german farms.storage mites are important sources of airborne allergens, especially on farms. a mite survey was conducted in the working environments of 121 farms in five regions of germany. of 859 samples, 743 (86.4%) contained mites, representing 547,857 astigmatic mites in 723 g of dust and 34,501 prostigmatic mites in 469 g of dust. dust samples from nine sites of each farm were collected and analyzed. ninety-three percent of all mites belonged to the order astigmata (storage- and house-dust-mite species) ...19979450144
house dust mite fauna in turkey.storage and house dust mites (hdm) appear to be important allergen sources in sensitive patients. the acarofauna of dust samples collected from 133 homes located in five regions of anatolia with different weather conditions were evaluated. hdm and/or storage mites were found in 32 samples. twenty of them were identified as containing d. pteronyssinus and d. farinae, and 21 had storage mites. lepidoglyphus destructor and gamasidae were the most frequently found, followed by cheyletus eraditus, gl ...19979491198
expression of two isoforms of lep d 2, the major allergen of lepidoglyphus destructor, in both prokaryotic and eukaryotic systems.the dust mite lepidoglyphus destructor is a major cause of allergic diseases among farmers. we have previously cloned and sequenced two isoforms of the major allergen lep d 2 (formerly designated lep d 1) and found significant homology to group 2 allergens of the house dust mite species dermatophagoides. we now report on the production and characterization of recombinant lep d 2.19989756203
ige binding capacity of synthetic and recombinant peptides of the major storage mite (lepidoglyphus destructor) allergen, lep d 2.lepidoglyphus destructor is an important non-pyroglyphid mite species in europe and a dominant allergen in farming environments. the major allergen of l. destructor, lep d 2, is a protein of 13.2 kd that is recognised by about 90% of sera rast positive to this mite species.19989831803
identification and purification of a 16-kda allergen from psoroptes ovis (acarina: psoroptidae).psoroptes ovis, (hering), the sheep scab mite, is the causative agent of an allergic dermatitis of sheep and cattle. recent studies of the host immune response to this ectoparasite have provided information that suggests control may be achieved by immune intervention. a significant effector in protection of the host from clinical lesions is host behavioral grooming. host grooming is believed to be intensified by a pruritic immediate hypersensitivity response to mite allergens. knowledge of poten ...199910534947
sensitization to non-pyroglyphid mites in urban population of croatia.determination of prevalence of sensitization to dermatophagoides pteronyssinus, dermatophagoides farinae (pyroglyphid mites), lepidoglyphus destructor, and tyrophagus putrescentiae (non-pyroglyphid mites) in urban continental croatia.200010810168
serum eosinophil cationic protein in asthma of farmers.there is need for relevant markers of bronchial inflammation in epidemiologic studies of asthma. serum eosinophil cationic protein (ecp) is a marker of eosinophil inflammation and asthma activity. we have studied serum ecp in atopic farmers with current asthma, in non-atopic asthmatics and in non-asthmatic, non-atopic controls.200010817397
factors influencing the clinical picture and the differential sensitization to house dust mites and storage mites.sensitization to house dust mites and storage mites has been studied in a number of papers, but several environmental factors and clinical conditions that differently affect sensitization to these mites are still controversial. the purpose of this study was to determine the influence of climatic conditions, occupation and patient age in the differential sensitization to house dust and storage mites, and also to search for possible different symptoms caused by each group of mites. eighty patients ...200011039840
cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology.the dust mite lepidoglyphus destructor is a common species in europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. one major allergen, lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of l. destructor-sensitized subjects. additional recombinant allergens are needed to obtain a reliable diagnostic tool for l. destructor allergy. the aim of this s ...200111168362
sensitization to different mite species in german farmers: clinical aspects.various mite species referred to collectively as house dust and storage mites are recognized worldwide as a cause of allergic airway disease. our study aimed to investigate the frequency of sensitization and potential importance of mite species in farmers using a broad mite spectrum. a total of 86 german farmers with rhinitis and/or asthma were studied by skin prick testing and/or enzyme allergosorbent test (east) with the following mites: blomia tjibodas, blomia tropicalis, blomia kulagini, gly ...200011206935
allergenic cross-reactivity between the nematode anisakis simplex and the dust mites acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae, and dermatophagoides pteronyssinus.the nematode anisakis simplex is a common parasite on fish and other seafood. it is considered to be a food allergen and to induce ige-mediated reactions. allergenic cross-reactivity between a. simplex and other nematodes has been reported, as has cross-reactivity with arthropods: red mosquito larvae and german cockroach. we have here studied the allergenic relationship between a. simplex and four different dust-mite species.200111421925
work-related skin symptoms and type i allergy among eastern-polish farmers growing hops and other crops.73 eastern-polish farmers growing hops and other crops were examined. they were questioned by a dermatologist and subsequently skin prick-tested with allergens of hops, grain dust, straw dust, hay dust, storage mites, and antigens of microorganisms typical for farm environment. results: 14 farmers (19.2%) complained of work-related skin symptoms, caused most often by hops (11%), followed by grain (5.6%), hay (5.5%) and straw (4.1%). five farmers (6.8%) complained of hand dermatitis, four (5.5%) ...200111426925
the occurrence of mites in cereal-based foods destined for human consumption and possible consequences of infestation.seven categories of cereal-based food products purchased at food retail outlets in uk were examined for the presence of mites by analysing 20 g samples using a flotation method. mites were found in 21% of 571 samples, which were examined soon after purchase, and in 38% of 421 samples, derived from the 571 samples which were examined after 6 weeks of storage in volunteers' homes. most of the samples where mites were detected had fewer than five mites. however, a few samples contained more than 20 ...200111523916
occurrence of storage mites in finnish farming environments.qualitative and semi-quantitative microscopic analyses were performed on the mite populations in dust samples collected from the byres and hay and grain storages of 11 farms in eastern finland. storage mites were found in all farms. the mite fauna was more abundant in the byres and hay storages than in the grain storages. the most common species or genera were acarus siro, acarus farris, glycyphagus domesticus, lepidoglyphus destructor, cheyletus eruditus, tydeus spp., and tarsonemus spp. the re ...19827137517
t cell responses to recombinant isoforms, synthetic peptides and a mutant variant of lep d 2, a major allergen from the dust mite lepidoglyphus destructor.the dust mite lepidoglyphus destructor is an important cause of allergic reactions to dust, especially in farming environments. two isoforms, recombinant (r)lep d 2.01 and rlep d 2.02, of the major allergen lep d 2, have previously been expressed as recombinant proteins. these isoforms differ 10.4% at the amino acid level. furthermore, a mutant form of lep d 2.01 (rlep d 2.6cys) with a highly reduced ige reactivity, has also been produced.200111737040
atopy, allergic diseases and work-related symptoms among students of agricultural schools: first results of the lublin study.136 eastern-polish farming students (51 females and 85 males, aged 16-23 years) underwent clinical examination, skin prick tests with common and farm-specific allergens, total ige measurement and phadiatop test. atopy was found in 35.3% (95% ci: 27.3-43.3%) of students. for allergic skin diseases, the point prevalence was 5.9%, the lifetime prevalence 28.7%; for allergic rhinitis 12.7% and 16.4%; for asthma 2.2% and 8.8% respectively. 56 students (41.2%) complained of work-related symptoms; most ...200111748886
sensitivity patterns to house dust mites and forage mites in atopic dogs: 150 cases.this study investigated intradermal test reactions to extracts of six species of mites in 150 dogs with atopic dermatitis. at least one positive reaction was seen in 120 animals (80%). dermatophagoides farinae attracted the highest number of positive reactions (108 dogs, 90% of dogs and 72% of atopic dogs showing positive reactions). positive reactions to other mites were not uncommon, with many dogs testing positive for dermatophagoides pteronyssinus (32% of dogs tested), acarus siro (35%), tyr ...200211896969
cloning and sequence analysis of a cdna encoding pso o ii, a mite group ii allergen of the sheep scab mite (acari: psoroptidae).psoroptes ovis (hering), the sheep scab mite, is responsible for psoroptic scabies of cattle and sheep. reverse translation of 30 n-terminal amino acids of the major p. ovis allergen, previously chosen as a candidate immunogen and identified as a 16 kda protein yielded a degenerate sequence used to design oligodeoxynucleotide polymerase chain reaction (pcr) primers. use of the pcr primers with a p. ovis cdna library succeeded in amplification of a 90 bp cdna gene fragment that was cloned, sequen ...200211931040
sensitization to dermatophagoides siboney, blomia tropicalis, and other domestic mites in asthmatic patients.mite species adapted to warm, humid climates are commonly found in house dust in the tropics. in cuba, dermatophagoides pteronyssinus, d. siboney, and blomia tropicalis are the most common and abundant mite species in house dust. to investigate the pattern of sensitization of cuban asthmatic patients to common mite species, we skin-prick-tested (spt) 148 patients with a clinical history of asthma and possible mite allergy, and determined specific ige antibodies against mite allergens (d. pterony ...19968863927
mite sensitivity and exposure in the city of santa fe, argentina.little information is available on mite allergen levels and rates of sensitization to domestic mites in south america. the purpose of this study was to establish the rate of sensitization to domestic mites in asthmatics and measure mite allergen levels in pillows and mattresses of mite allergic individuals in santa fe, argentina. dust samples were collected from the pillows and mattresses of 56 asthmatics (24 females and 32 males, mean age 17.6 +/- 11.5 years), who had been previously skin teste ...19968959537
evaluation of the acarofauna of the domiciliary ecosystem in juiz de fora, state of minas gerais, brazil.from august 1999 to january 2000, samples of house dust were collected from 160 domiciles in the city of juiz de fora, state of minas gerais, brazil. in 36 of these domiciles kitchen samples were obtained. prevalence rate was 77.5%, varying according to the geographical sector. there were found 2,278 specimens of mites, with 1,530 (67.2%) in the adult stage and 748 (32.8%) in immature forms. the main species found were dermatophagoides pteronyssinus, d. farinae, euroglyphus maynei, blomia tropic ...200111685254
studies on house dust mites in great cairo, egypt.nowadays, house dust mites have showed themselves as one of the health problem worldwide. they have to be considered in the differential diagnosis of allergy particularly in children. house dust mites were collected from eight different areas in great cairo. nine species of mites were recovered from indoors. in order of abundance, they were dermatophagoides pteronyssinus, d. farinae, tyrophagous putrescentiae, acarus siro, cheyletus malaccensis, blomia kulagini, acheles graciles, ornithonyssus b ...199912561892
associations of fc epsilon r1-beta polymorphisms with immunoglobin e antibody responses to common inhalant allergens in a rural population.polymorphisms within the beta subunit of the high-affinity receptor for ige (fc epsilon r1-beta ) on chromosome 11q13 have been related to atopy and asthma and the lymphotoxin alpha (lt alpha) gene on chromosome 6 is implicated in asthma.200212047428
the 3 mm skin prick test (spt) threshold criterion is not reliable for tyrophagus putrescentiae: the re-evaluation of spt criterion to dust mites.the mean wheal diameter >/= 3 mm is the usual criterion for positive skin prick test (spt) reaction to dust mites. the study assessed the accuracy of this spt criterion with respect to specific ige values of above 0.35 kua/l (+ sige).200212464048
in vivo and in vitro sensitization to domestic mites in german urban and rural allergic patients.sensitization to domestic mites is common in germany. the main objectives of this study were (1) to establish the rate of skin test sensitivity to dermatophagoides pteronyssinus, acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae in 512 consecutive patients evaluated for upper and/or lower respiratory complaints; (2) to verify how many of the patients with a positive skin test to at least one of the three storage mites were also skin test positive to d. pteronyssinus; and (3) to ...200212530116
biologic activity of dermatophagoides siboney and blomia tropicalis allergens in exposed and unexposed mite-allergic individuals. effect of patient selection on the biologic standardization of mite extracts.this study aimed to investigate the influence of patient selection criteria, i.e., mite-allergic individuals exposed and not exposed to dermatophagoides siboney and blomia tropicalis, on the biologic activity of mite extracts. determination of the potency of mite extracts in vivo requires selection of patients with a clinical history of mite allergy. in scandinavia, there are some anamnestic criteria for mite allergy, whereas in the tropics, where patients are continuously exposed to high levels ...199910371100
ige-mediated allergy to lepidoglyphus destructor in an urban population--an epidemiologic study.the aim of this study was to estimate the prevalence of ige-mediated allergy to lepidoglyphus destructor and its clinical importance in reykjavik, iceland.199910485393
exposure levels and skin reactivity to german cockroach (blattella germanica) in croatia.to evaluate german cockroach (blattella germanica) allergen levels in house dust and skin reactivity to german cockroach in adult atopic and non-atopic subjects in inland areas of croatia.200314652891
sequence tag catalogs of dust mite-expressed genomes: utility in allergen and acarologic studies.dust mites are a major source of indoor allergens. they contain a large number of components that react with immunoglobulin (ig) e in individuals with allergies and are capable of inducing sensitization, and allergic respiratory and cutaneous diseases. with a significant proportion of the population affected in some way by mite allergies, it is essential that we improve our understanding of these organisms so that control strategies could be defined and its allergens better understood. thus, we ...200415651897
mites and fungi in heavily infested stores in the czech republic.toxigenic and allergen-producing fungi represent a serious hazard to human food and animal feed safety. ninety-four fungal species were isolated from mite-infested samples of seeds taken from czech seed stores. fungi were isolated from the surface of four kinds of seeds (wheat, poppy, lettuce, and mustard) and from the gut and external surface of five species of mites (i.e., acarus siro l., 1758, caloglyphus rhizoglyphoides (zachvatkin, 1973), lepidoglyphus destructor (schrank, 1781), tyrophagus ...200415666776
allergen-specific sensitization in asthma and allergic diseases in children: the study on farmers' and non-farmers' children.farmers' children are less frequently sensitized to common allergens than the non-farmers' children, but less is known about their sensitization to other allergens and its association with clinical diseases.200515725186
prevalence of serum ige to storage mites in a southwestern ohio population.storage mites of the families chortoglyphidae, glycyphagidae, echimyopodidae, and acaridae are commonly found in agricultural and nonagricultural environments. the prevalence of sensitization to these storage mites in the general population in the united states is unknown.200616498860
sensitization and exposure to house dust and storage mites in high-altitude areas of ecuador.few studies have addressed exposure and sensitization to mite allergens in andean countries.200617069110
[house dust mite in kutahya, turkey.]the aim of this study was to determine the prevalence and species of house dust mites (hdm) in kutahya (turkey). the prevalence of house dust mites (hdm) was found to be 18.05%. the following species were identified: tyrophagus putrescentiae, (43.96%), dermatophagoides pteronyssinus (31.03%), acarus siro (13.79%), lepidoglyphus destructor (1.72%), glycphagus domesticus (2.58%) and cheyletus spp. (1.72%). hdm were found from april to october and a high rate, from april to june. a very high rate o ...200517160837
enzymatic activity in extracts of allergy-causing astigmatid mites.many of the previously characterized allergens of house dust mites are known to be proteases, and this enzymatic activity is thought to contribute to their allergenicity. other astigmatid mites, including stored-product mites and the ectoparasitic itch mite, sarcoptes scabiei de geer, are also known to be allergenic, but little or nothing is known about their enzymatic activities. the purpose of this study was to characterize the enzymatic activities present in extracts of the parasitic itch mit ...200617162954
risk factors for sensitization to anisakis simplex: a multivariate statistical evaluation.anisakis simplex is a nematode belonging to the anisakidae family. the ingestion of third stage larvae in uncooked or undercooked seafood may cause human diseases known as anisakiasis and anisakidosis. a total of 400 (159 atopic and 241 non-atopic) subjects living in an area of southern italy (bari district) were consecutively evaluated to identify the association of some factors (sex, age, atopy, consumption of uncooked seafood and sensitization to dust mites) with the risk of anisakis simplex ...200617166406
acaroid mite, intestinal and urinary acariasis.to investigate epidemiology and pathogenic mite species of intestinal and urinary acariasis in individuals with different occupations.200312679953
cloning and characterisation of two ige-binding proteins, homologous to tropomyosin and alpha-tubulin, from the mite lepidoglyphus destructor.the dust mite lepidoglyphus destructor is a major source of mite allergy in european rural environments, but it also causes allergy in urban populations around the world. we have previously cloned, sequenced and expressed several allergens from l. destructor (lep d 2, lep d 5, lep d 7 and lep d 13). the aim of this study was to identify and clone additional allergens from l. destructor, and to evaluate their ige-binding reactivities.200312740526
contribution of disulphide bonds to antigenicity of lep d 2, the major allergen of the dust mite lepidoglyphus destructor.to study the contribution of the 3 disulphide bonds in the major allergen lep d 2 to the antigenic structure, site-directed mutagenesis was performed. mutants with one or more cysteine residues altered were constructed with a histidine residue tag for purification purposes and expressed as recombinant proteins in e. coli. seven mutants were analysed: 3 single mutants (cys 8, cys 21 and cys 72), 3 double mutants (cys 8-117, cys 21-16 and cys 72 77) and one mutant with all 6 cysteines altered (6 c ...199810068036
nasal complaints and signs of disease in farmers--a methodological study.the methods used in this study are suitable for field studies that involve examinations of groups of workers. for individual examinations, there is no gold standard method that can discriminate work-related discomfort from other causes of rhinitis.200817917841
[house dust mites in the etiology of allergic diseases].one of the more important etiological factors of allergies are mites occuring in the closest vicinity of humans, i.e. in the house dust and in the stored foodstuffs. the most important are the species representing the families: pyroglyphidae (mainly dermatophagoides pteronyssinus, d. farinae, and euroglyphus maynei), glycyphagidae (e.g. lepidoglyphus destructor, blomia sp.), and acaridae (e.g. tyrophagus putrescentiae, acarus siro). mites shed an abundance of allergenic proteins. particularly ab ...200617633128
digestive function of lysozyme in synanthropic acaridid mites enables utilization of bacteria as a food source.the activity of lysozyme, the enzyme that hydrolyzes peptidoglycan in g(+) bacterial cell walls, was detected in whole mite extracts (wme) and in spent growth medium extracts (sgme) of 14 species of synanthropic mites (acari: acaridida). the adaptation of lysozyme for digestive activity and bacteriophagy was based on: (i) high lysozyme activity in sgme, and (ii) the correlation of maximum lysozyme activity at acidic ph values, corresponding to ph in the ventriculus and caeca. we show that the di ...200818357505
comparison between an intradermal skin test and allergen-specific ige-elisa for canine atopic dermatitis.the aim of this study was to compare the results of an intradermal skin test (idst) with those of an allergen-specific ige-elisa in 210 dogs with atopic dermatitis. all the dogs had a clinical diagnosis of atopic dermatitis and underwent an idst. the sera of all dogs were analysed for allergen-specific ige by elisa using the monoclonal antibody d9 against dog ige. idst was used as the standard assay. in both methods, the following antigens provided a positive test result: dermatophagoides farina ...200818429502
importance of house dust and storage mites in canine atopic dermatitis in the geographic region of galicia, spain.sensitisation to mites is frequent in atopic dogs. the main mite genus involved in canine atopic dermatitis is dermatophagoides. the importance of storage mite allergens in dogs has been controversial. the aim of this study was to evaluate the sensitisation rates against storage mites (lepidoglyphus destructor and tyrophagus putrescentiae) and house dust mites (dermatophagoides farinae and d. pteronyssinus) in atopic dogs from galicia, a highly humid and temperate region of spain, using a fcepsi ...200818669243
comparative mites and cockroaches sensitization study in three cities of mexico.in mexico there are few research papers dealing with mites and cockroaches sensibilizing in patients suffering from respiratory allergies and even less susceptibility comparative studies to the same between cities having different climatic and geographic characteristics.200819157220
morphological diagnosis of the eggs of stored-products mites.eggs of 13 species of common, economically important stored-product pest mites (acarus siro, a. gracilis, tyrophagus putrescentiae, t. brevicrinatus, tyroborus lini, aleuroglyphus ovatus, caloglyphus redikorzevi, c. oudemansi, lepidoglyphus destructor, glycyphagus domesticus, aeroglyphus robustus, chortoglyphus arcuatus and carpoglyphus lactis) from four families (acaridae, glycyphagidae, chortoglyphidae, carpoglyphidae) were studied in order to build a diagnostic key. the morphological study de ...200919252823
bacillus thuringiensis var. tenebrionis control of synanthropic mites (acari: acaridida) under laboratory conditions.bacillus thuringiensis (bt) toxins present a potential for control of pest mites. information concerning the effect of bt and its possible application to the biocontrol of synathropic mites is rare. the toxic effect of bacillus thuringiensis var. tenebrionis producing cry3a toxin was tested on the mites acarus siro l., tyrophagus putrescentiae (schrank), dermatophagoides farinae hughes, and lepidoglyphus destructor (schrank) via feeding tests. fifty mites were reared on bt additive diets in conc ...200919381844
sensitization to different mite species in german farmers: in vitro analyses.allergic airway diseases are often caused by house dust mites (hdm) and storage mites (sm), respectively, and we were recently able to demonstrate that symptomatic german farmers are frequently sensitized to different mite species. the present study aimed to obtain information on the protein and immunobiochemical characteristics of the extracts of the following mites: dermatophagoides pteronyssinus, dermatophagoides farinae, acarus siro, acarus farris, lepidoglyphus destructor, tyrophagus putres ...200312861848
assessment of cross-reactivity among five species of house dust and storage mites.in vitro cross-reactivity among two house dust (dermatophagoides farinae, d. pteronyssinus) and three storage (acarus siro, tyrophagus putrescentiae, lepidoglyphus destructor) mites was examined in 20 mite-sensitive dogs with natural occurring atopic dermatitis (group a), 13 high-ige beagles experimentally sensitized to d. farinae (group b), and five healthy beagles (group c). intradermal testing (idt) and serology for allergen-specific ige demonstrated that co-sensitization for all possible pai ...200818336423
the importance of starch and sucrose digestion in nutritive biology of synanthropic acaridid mites: alpha-amylases and alpha-glucosidases are suitable targets for inhibitor-based strategies of mite control.the adaptation of nine species of mites that infest stored products for starch utilization was tested by (1) enzymatic analysis using feces and whole mite extracts, (2) biotests, and (3) inhibition experiments. acarus siro, aleuroglyphus ovatus, and tyroborus lini were associated with the starch-type substrates and maltose, with higher enzymatic activities observed in whole mite extracts. lepidoglyphus destructor was associated with the same substrates but had higher activities in feces. dermato ...200919480003
molecular and biochemical properties of storage mites (except blomia species).in recent years, the allergological importance of different mite species not belonging to the family pyroglyphidae has been demonstrated. these mites, commonly named storage mites, include lepidoglyphus destructor, glycyphagus domesticus, tyrophagus putrescentiae, acarus siro, aleuroglyphus ovatus, suidasia medanensis and thyreophagus entomophagus. several allergens from these species have been purified, sequenced and cloned. many of these allergens have shown sequence homology and a biological ...200718220992
[acarological faunistic and statistical analysis of dust from various compartments of human dwellings in north-western poland].the aim of this paper was to compare the species composition of mites in dust samples collected at different compartments of human dwellings of north-western (nw) poland. in 30 urban apartments (szczecin, police, pyrzyce and łobez) and rural quarters (przelewice, brzesko szczecińskie, bylice, krasne, kakolewice, wierzchowo, przeradz, kłodzino) from nw poland, 150 samples of dust were collected at 5 locations: bed area, bedroom carpet, sitting area in the living room, hall carpet, and kitchen flo ...201020450008
sensitization to dermatophagoides, blomia tropicalis, and other mites in atopic patients.house dust mites (hdms) are important causes of persistent allergic diseases, such as asthma and rhinitis. various types of mites are found in the house dust of many countries, including israel.201020846087
house dust and storage mite extracts influence skin keratinocyte and fibroblast function.the bodies of allergy-causing dust and storage mites likely contain many bioreactive molecules, including some that are allergenic. these molecules may penetrate the epidermis and dermis of the skin. however, little is known about the effects that most of the molecules from mites have on the function of cells in the skin, the overall inflammatory and immune reactions and the manifestation of allergic disease. the purpose of this research was to determine the response of cultured skin cells (kera ...200817703098
[atopic allergy in an icelandic urban population 20-44 years of age.]this is a part of a second stage of ec respiratory health survey and the aim of this study is to estimate the prevalence of atopic allergy among urban population of men and women aged 2cm4 years, living in the capital reykjavik and suburbs. altogether 800 individuals were invited of whom 570 participated (77%). all participants answered questionnaire and 540 individuals were skin prick tested by 12 allergens. 20.5% had positive skin tests, defined as at least one allergen positive by a weal reac ...199520065461
passive airborne dust sampling to assess mite antigen exposure in farming environments.the aim of this study was to estimate mite antigen exposure in farming environments by passive sampling of airborne dust. antigen concentrations were measured with enzyme immunoassays specific for three storage mites (sm): acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae and the house dust mite (hdm) dermatophagoides pteronyssinus. dust samples were collected with electrostatic dust fall collectors (edcs) in three different areas of cattle farms. edcs were placed in cow stables (w ...201121842065
Seasonal Dynamics of House Dust Mites in Dust Samples Collected From Sleeping Places in North-Western Poland.The most common families of mites found in house dust are Pyroglyphidae, Glycyphagidae and Acaridae; all are a source of many antigens responsible for allergic diseases. The aim of this study was to examine the seasonal dynamics of allergenic mite populations in dust samples collected from sleeping places in apartments in north-western Poland. The mites were isolated from the dust using a saturated saline floating method. In 132 dust samples we determined: Dermatophagoides farinae, Dermatophagoi ...201121824362
Modelling and Bioinformatics Analysis of the Dimeric Structure of House Dust Mite Allergens from Families 5 and 21: Der f 5 Could Dimerize as Der p 5.Allergy represents an increasing thread to public health in both developed and emerging countries and the dust mites Dermatophagoides pteronyssinus (Der p), Blomia tropicalis (Blo t), Dermatophagoides farinae (Der f), Lepidoglyphus destructor (Lep d) and Suidasia medanensis (Sui m) strongly contribute to this problem. Their allergens are classified in several families among which families 5 and 21 which are the subject of this work. Indeed, their biological function as well as the mechanism or e ...201222208271
Detection and Identification of Species-Specific Bacteria Associated with Synanthropic Mites.Internal bacterial communities of synanthropic mites Acarus siro, Dermatophagoides farinae, Lepidoglyphus destructor, and Tyrophagus putrescentiae (Acari: Astigmata) were analyzed by culturing and culture-independent approaches from specimens obtained from laboratory colonies. Homogenates of surface-sterilized mites were used for cultivation on non-selective agar and DNA extraction. Isolated bacteria were identified by sequencing of the 16S rRNA gene. PCR amplified 16S rRNA genes were analyzed b ...201122057398
the effect of antibiotics on associated bacterial community of stored product mites.bacteria are associated with the gut, fat bodies and reproductive organs of stored product mites (acari: astigmata). the mites are pests due to the production of allergens. addition of antibiotics to diets can help to characterize the association between mites and bacteria.201425387104
trophic determinants of hypopus induction in the stored-product mite lepidoglyphus destructor (acari: astigmata).a unique (synapomorphic) characteristic of astigmatic mites is the heteromorphic deuteronymph also called hypopus. it is a non-feeding and facultative instar between protonymph and tritonymph. the hypopus is adapted for dispersal and sometimes also for dormancy, as in lepidoglyphus destructor. the experiments reveal a correlation between the composition of the foodstuff, the duration of development of homomorphic instars, the mortality of protonymphs and the production of hypopodes. as food qual ...200314580061
assessment of monitoring methods for early detection of three species of storage mite in bulk oilseed rape.two types of trap, the bt trap and pc(tm) pitfall cone trap, were evaluated against the most widely used method of sampling and sieving for their ability to detect low numbers of storage mites (acarus siro, tyrophagus longior and lepidoglyphus destructor) in bulk oilseed rape, under uk maritime conditions. in addition, a synthetic lure (2-nonanone) was incorporated into the two types of trap to assess whether its presence improved trapping performance. the bt traps and pc traps were significantl ...200516180079
comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites.microplate assays with 96 wells were optimized to screen proteolytic activities in mite homogenates. whole-mite extracts of acarus siro, aleuroglyphus ovatus, tyrophagus putrescentiae, tyroborus lini, carpoglyphus lactis, lepidoglyphus destructor, and dermatophagoides farinae exhibited non-specific proteolytic activity in buffers from ph 2 to 12, and three peaks of highest activity at ph 3, 5-6, and 10 were distinguished. the reducing agent tris(2-carboxyethyl)phosphine hydrochloride decreased g ...201020936642
[airborne immediate allergy in mid-north iceland.]objective: to investigate main causes of airborne immediate allergy in a region of one primary health care center with about 17,000 inhabitants in the northern part of iceland, both in general and with special reference to diagnoses. material and methods: totally 600 individuals with symptoms indicating airborne immediate allergy were investigated under the periode 1988-1995. skin prick test (spt) was used in a standardized way and with standardized solutions. histamine 10 mg/ml was used as a po ...199819667457
sensitization of children to storage mites in kutahya, turkey.specific ige against acarus siro, glycphagus domesticus, tyrophagus putrescentiae, and lepidoglyphus destructor have been investigated by elisa in sera of 92 children. of them, 41 were found to be specific ige positive (> or = 0.35 iu/ml) against at least one of house dust mite species, dermatophagoides pteronyssinus and dermatophagoides farinae, by an immunoblot. in 65.9% of the dust mite-sensitized children, specific ige against at least one of these mite species was found. sensitization level ...200919967087
temperature preference and respiration of acaridid mites.the thermal preferences in a grain mass and respiration at various temperatures in mites (acari: acarididae) of medical and economical importance [acarus siro (l. 1758), dermatophagoides farinae hughes 1961, lepidoglyphus destructor (schrank 1871), and tyrophagus putrescentiae (schrank 1781)] were studied under laboratory conditions. based on the distribution of mites in wheat, triticum aestivum l., grain along a thermal gradient from 10 to 40 degrees c, l. destructor, d. farinae, and a. siro we ...201021309251
Displaying items 1 - 100 of 200