| thioredoxin activity in the c terminus of phalaris s protein. | self-incompatibility in the grass phalaris coerulescens is controlled by two genes s and z. isolation and sequencing of two s alleles showed that they encode proteins that are highly conserved at the c terminus with significant homology to thioredoxin h proteins. in particular, the residues in and around the active site of thioredoxin, trp-cys-gly-pro-cys, are perfectly conserved. the c terminus of the s protein has been expressed in eschericia coli and purified to homogeneity on ni-nta resin. f ... | 1995 | 7655504 |
| a self-fertile mutant of phalaris produces an s protein with reduced thioredoxin activity. | gametophytic self-incompatibility in the phalaris coerulescens is controlled by two unlinked genes, s and z. isolation of the s gene from the pollen of this grass species indicated that the c terminus has significant homology with thioredoxin h proteins. the protein from the c terminus, expressed in escherichia coli, exhibits thioredoxin-life activity. this paper demonstrates that the c terminus of the s protein from an s complete mutant shows significant reduction in thioredoxin activity when c ... | 1996 | 8811864 |
| suspected blue canary grass (phalaris coerulescens) poisoning of horses. | | 1999 | 10494401 |
| cloning and expression of a distinct subclass of plant thioredoxins. | mrnas encoding a novel thioredoxin were isolated from pollen rna of lolium perenne (lptrx), hordeum bulbosum (hbtrx), phalaris coerulescens (ptrx) and secale cereale (sctrx). the cdnas contain a single orf of 393 bp encoding a protein of 131 amino acids. the predicted proteins showed highest homology to plant thioredoxins of the h class yet form a distinct subgroup that is characterized by a high level of sequence conservation (95.4-97.7% identity). genbank searches revealed additional members o ... | 2000 | 11106422 |
| a simple tae-based method to generate large insert bac libraries from plant species. | large insert libraries are valuable tools for the positional cloning of genes of interest, physical mapping of chromosomes, comparative genomics, and molecular breeding. there are five types of large dna insert libraries: cosmid, yeast artificial chromosomes (yacs), bacteriophage p1, bacterial artificial chromosomes (bacs) and p1-derived artificial chromosomes (pacs) libraries. of these libraries, bac libraries are the most widely used due to their ease of manipulation, large insert size, and st ... | 2009 | 19347648 |
| transgenic barley with overexpressed ptrx increases aluminum resistance in roots during germination. | a transgenic barley line (lsy-11-1-1) with overexpressed phalaris coerulescens thioredoxin gene (ptrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/l alcl(3) on filter paper. the results show that (1) compared with the non-transgenic barley, lsy-11-1-1 had enhanced root growth, although both were seriously inhibited after alcl(3) treatment; (2) the degree of protein oxidation and loss ... | 2010 | 21043055 |