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phage specificity and lipopolysaccarides of stem- and root-nodulating bacteria (azorhizobium caulinodans, sinorhizobium spp., and rhizobium spp.) of sesbania spp.phage susceptibility pattern and its correlation with lipopolysaccharide (lps) and plasmid profiles may help in understanding the phenotypic and genotypic diversity among highly promiscuous group of rhizobia nodulating sesbania spp.; 43 phages were from two stem-nodulating bacteria of s. rostrata and 16 phages were from root-nodulating bacteria of s. sesban, s. aegyptica and s. rostrata. phage susceptibility pattern of 38 sesbania nodulating bacteria was correlated with their lps rather than pla ...200817989956
isolation and characterization of a novel cross-infective rhizobia from sesbania aculeata (dhaincha).the sesbania has been widely used as green manure to improve the productivity of several crops. sinorhizobium saheli strain (sb2) was isolated from the root nodule of sesbania aculeata. the tn5 mutants (300) of sb2 were generated and studied for their nodulation efficiencies in its specific and cross-infective host plants. the mutant, sb2m3, was found to have two- and four fold higher nodulation efficiency than wild type in parent host and nonspecific host plant, respectively. sb2m3 differed fro ...200817896133
the sesbania root symbionts sinorhizobium saheli and s. teranga bv. sesbaniae can form stem nodules on sesbania rostrata, although they are less adapted to stem nodulation than azorhizobium caulinodans.sesbania species can establish symbiotic interactions with rhizobia from two taxonomically distant genera, including the sesbania rostrata stem-nodulating azorhizobium sp. and azorhizobium caulinodans and the newly described sinorhizobium saheli and sinorhizobium teranga bv. sesbaniae, isolated from the roots of various sesbania species. a collection of strains from both groups were analyzed for their symbiotic properties with different sesbania species. s. saheli and s. teranga bv. sesbaniae st ...199716535538
diversity in a promiscuous group of rhizobia from three sesbania spp. colonizing ecologically distinct habitats of the semi-arid delhi region.sesbania-rhizobia associations have immense significance in soil amelioration programs for diverse habitats. diversity in symbiotic properties, lps profiles, sym plasmid and rhizobiophage sensitivity of 28 root- and stem-nodulating bacterial isolates of three sesbania species (s. sesban, s. aegyptica and s. rostrata) inhabiting six ecologically distinct sites of semi-arid delhi region was analyzed. the isolates were highly promiscuous among the symbiotic partners (sesbania spp.). the root nodule ...200515636748
the genus name ensifer casida 1982 takes priority over sinorhizobium chen et al. 1988, and sinorhizobium morelense wang et al. 2002 is a later synonym of ensifer adhaerens casida 1982. is the combination "sinorhizobium adhaerens" (casida 1982) willems et al. 2003 legitimate? request for an opinion.the synonymy of the genera ensifer and sinorhizobium was recently reported, but it was proposed that the later-named genus, sinorhizobium, take priority in nomenclature. there is no justification in the international code of nomenclature of bacteria (prokaryotes) for this step; ensifer is the correct name of the genus, with ensifer adhaerens as the type species. species previously allocated to sinorhizobium are here proposed as the new combinations ensifer arboris, ensifer fredii, ensifer kostie ...200314657154
genetic diversity of fast-growing rhizobia that nodulate soybean ( glycine max l. merr).the fast-growing rhizobium sp. strain ngr234, isolated from papua new guinea, and 13 strains of sinorhizobium fredii, isolated from china and vietnam, were fingerprinted by means of rapd, rep, eric and ardra. eric, rep and rapd markers revealed a considerable genetic diversity among fast-growing rhizobia. chinese isolates showed higher levels of diversity than those strains isolated from vietnam. ardra analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, ...200312802480
[study on polyphasic taxonomy of rhizobia isolated from lespedeza species].the diversity of rhizobia isolated from lespedeza spp. was determined on the basis of numerical analysis of phenotypic characteristics, sodium dodecyl sulfate-polyacrylarmide gel electrophoresis (sds-page) of proteins, dna-dna homology and restriction fragment length polymorphism (rflp) analysis of 16s-ribosomal dna genes. according to numerical analysis of 125 phenotypic characteristics, strains were divided into two groups at a similarity level of 67%. group i included all the fast-growing str ...199912555566
characterization of rhizobia that nodulate legume species of the genus lespedeza and description of bradyrhizobium yuanmingense sp. nov.legume species belonging to the genus lespedeza are annual or perennial herb or shrub plants that grow in the northern hemisphere. they are known for the formation of root nodules, but little information is available about their microsymbionts. in this study, 58 root-nodule isolates from lespedeza spp., obtained from china and the usa, were characterized using numerical taxonomic analysis of phenotypic features, sds-page analysis of whole-cell proteins, dna-dna hybridization, 16s rrna gene seque ...200212508891
the diversity of rhizobia nodulating beans in northwest argentina as a source of more efficient inoculant strains.the common bean (phaseolus vulgaris l.) is cultivated widely in central and south america and particularly in the northwest of argentina. in order to describe the diversity of the common bean nodulating rhizobial population from the bean producing area in northwest argentina (nwa), a collection of about 400 isolates of common beans recovered from nodules and soil samples from nwa were characterized by using nifh-pcr, analysis of genes coding for 16s rrna and nodc, and rep-fingerprinting, respect ...200111566389
analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system.previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ...200010758890
sinorhizobium arboris sp. nov. and sinorhizobium kostiense sp. nov., isolated from leguminous trees in sudan and kenya.sds-page of total bacterial proteins was applied to the classification of 25 sudanese and five kenyan strains isolated from the root nodules of acacia senegal and prosopis chilensis. twenty strains were also studied by multilocus enzyme electrophoresis (mlee) and the whole 16s rrna gene was sequenced from two strains representing the two major clusters. these results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, dna-dna dot-blot hyb ...199910555313
three phylogenetic groups of noda and nifh genes in sinorhizobium and mesorhizobium isolates from leguminous trees growing in africa and latin america.the diversity and phylogeny of noda and nifh genes were studied by using 52 rhizobial isolates from acacia senegal, prosopis chilensis, and related leguminous trees growing in africa and latin america. all of the strains had similar host ranges and belonged to the genera sinorhizobium and mesorhizobium, as previously determined by 16s rrna gene sequence analysis. the restriction patterns and a sequence analysis of the noda and nifh genes divided the strains into the following three distinct grou ...19989464375
rapid identification of medicago nodulating strains by using two oligonucleotide probes complementary to 16s rdna sequences.symbiotic bacteria associated with the medicago genus are separated into two closely related species named sinorhizobium meliloti and sinorhizobium medicae. to discriminate rapidly between these two bacterial species, two 15-base dna probes, 16smfs and 16smed, were designed from the alignment of 16s rdna sequences to differentiate s. meliloti from s. medicae. their specificities were evaluated by dot-blot hybridization experiments on 25 reference strains representing 13 species of rhizobium and ...19979336948
sinorhizobium medicae sp. nov., isolated from annual medicago spp.the taxonomic position of isolates of a new genomic species (designated genomic species 2) obtained from several annual medicago species and originating from different geographical locations was established through the results of phenotypic tests (including the results of auxanographic and biochemical tests and symbiotic properties) and 16s rrna phylogenetic inferences. a comparison of the complete 16s rrna sequence of a representative of genomic species 2 (strain a 321t [t = type strain]) with ...19968863426
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