| diol metabolism and diol dehydratase in clostridium glycolicum. | levels of the five enzymes involved in the fermentation of 1,2-ethanediol and 1,2-propanediol in the strictly anaerobic bacterium, clostridium glycolicum, were investigated. all enzymes with the exception of the first enzyme in the pathway, diol dehydratase, were found to be constitutive, stable to exposure to oxygen, and present in the cytosol. diol dehydratase was found to be extremely oxygen sensitive and strongly associated with the cell membrane. treatment with ionic and nonionic detergents ... | 1986 | 2936306 |
| solubilization of a membrane-bound diol dehydratase with retention of epr g = 2.02 signal by using 2-(n-cyclohexylamino)ethanesulfonic acid buffer. | a procedure for solubilization of the oxygen-labile, membrane-bound diol dehydratase from clostridium glycolicum with retention of enzymatic activity is described. the procedure involves sonication of crude membrane preparations anaerobically in 0.1 m 2-(n-cyclohexylamino)ethane-sulfonic acid (ches) buffer (ph 8.6-9.0) containing 2 mm dithiothreitol. the addition of dimethylsulfoxide (30%) and lysophosphatidylcholine (0.15 mg/ml) to the solubilization buffer resulted in a 10-fold increase in rec ... | 1987 | 3025865 |
| clostridium glycolicum in an addax. | | 1987 | 3590558 |
| isolation and characterization of a new bacterium carboxylating phenol to benzoic acid under anaerobic conditions. | a consortium of spore-forming bacteria transforming phenol to benzoic acid under anaerobic conditions was treated with antibiotics to eliminate the four clostridium strains which were shown to be unable to accomplish this reaction in pure culture and coculture. clostridium ghonii was inhibited by chloramphenicol (10 micrograms/ml), whereas clostridium hastiforme (strain 3) and clostridium glycolicum were inhibited by clindamycin (20 micrograms/ml), without the transformation of phenol being affe ... | 1996 | 8626321 |
| physiological ecology of clostridium glycolicum rd-1, an aerotolerant acetogen isolated from sea grass roots. | an anaerobic, h(2)-utilizing bacterium, strain rd-1, was isolated from the highest growth-positive dilution series of a root homogenate prepared from the sea grass halodule wrightii. cells of rd-1 were gram-positive, spore-forming, motile rods that were linked by connecting filaments. acetate was produced in stoichiometries indicative of an acetyl coenzyme a (acetyl-coa) pathway-dependent metabolism when rd-1 utilized h(2)-co(2), formate, lactate, or pyruvate. growth on sugars or ethylene glycol ... | 2001 | 11571179 |
| isolation of a cinnamic acid-metabolizing clostridium glycolicum strain from oil mill wastewaters and emendation of the species description. | a strictly anaerobic, gram-positive, motile, sporulated bacterium, designated strain cin5, was isolated from olive mill wastewaters after enrichment on cinnamic acid. the rod-shaped cells were slightly curved (0.4-1.1 x 2.0-15 microm) and occurred singly or in pairs. strain cin5 utilized a limited number of carbohydrates (glucose, fructose, maltose, sorbitol), grew optimally at 37 degrees c and at ph 7.3-7.5 and had a dna g+c content of 29.1+/-0.3 mol%. strain cin5 was very closely related to cl ... | 2001 | 11760946 |
| fermentation of ethylene glycol by clostridium glycolicum, sp. n. | gaston, lamont w. (national heart institute, national institutes of health, bethesda, md.) and e. r. stadtman. fermentation of ethylene glycol by clostridium glycolicum, sp. n. j. bacteriol. 85:356-362. 1963.-an anaerobic organism which utilizes ethylene glycol as a source of energy and carbon has been isolated from mud. it is a long (5 mu), slender, motile, gram-positive, spore-forming rod, with peritrichous flagellae. it grows well from 22 to 37 c at ph 7.4 to 7.6, and ferments glucose, fructo ... | 1963 | 13946772 |
| clostridium bartlettii sp. nov., isolated from human faeces. | seven obligately anaerobic, gram-positive, rod-shaped, spore-forming organisms isolated from human faecal specimens were characterized using phenotypic and molecular taxonomic methods. strains of the unidentified bacterium used carbohydrates as fermentable substrates, producing acetic acid, isovaleric acid and phenylacetic acid (paa) as the major products of glucose metabolism, and possessed a g +c content of approximately 29.8 mol%. comparative 16s rrna gene sequencing showed that the 7 strains ... | 2004 | 16701516 |
| volatilisation of metals and metalloids by the microbial population of an alluvial soil. | in order to assess the microbial contribution to the volatilisation of metal(loid)s by methylation and hydridisation in the environment, we focused on soils of different origin. here, we describe the biogenic production of volatile metal(loid) species of an alluvial soil with rather low metal(loid) contamination. the production of volatile metal(loid) compounds was monitored in soil suspensions kept under anaerobic conditions over an incubation time of 3 months. in the headspace of the samples, ... | 2007 | 16797907 |
| fatal clostridial enterotoxemia (clostridium glycolicum) in an ornate nile monitor (varanus ornatus). | enterotoxemia caused by clostridium glycolicum was identified as the cause of circulatory collapse and death in a female, 3-yr-old, captive-bred ornate nile monitor (varanus ornatus). anaerobic culture of fecal samples from 12 other monitor lizards from the collection failed to demonstrate the presence of this pathogen. | 2006 | 17312813 |
| clostridium glycolicum bacteremia in a bone marrow transplant patient. | we describe a case of clostridium glycolicum bacteremia and septic shock in an adult woman with a recent bone marrow transplant for relapsed hodgkin's disease. the bacterium was identified by 16s rrna gene sequencing. this is the first published report of the recovery of this organism from human clinical material. | 2007 | 17344364 |
| clostridium glycolicum isolated from a patient with otogenic brain abscesses. | we describe a case of brain abscesses with gas formation following otitis media, for which the patient treated himself by placing clay in his ear. several microorganisms, including clostridium glycolicum, were cultured from material obtained from the patient. this is the first report of an infection in an immunocompetent patient associated with this microorganism. | 2009 | 19109475 |
| effect of a single oral administration of lactobacillus plantarum dsmz 8862/8866 before and at the time point of weaning on intestinal microbial communities in piglets. | the aim of this study was to evaluate whether a single administration of two strains of lactobacillus plantarum (dsmz 8862 and 8866) either before or at the time point of weaning can influence the intestinal microbiota of piglets. a total of 176 piglets were allocated into five groups: control (lp0), administration of 5 x 10(9) or 5x10(10) cfu at day 25 of life (lp1, lp2) and administration of 5 x 10(9) or 5 x 10(10) cfu at day 28 of life (lp3, lp4). piglets were weaned on day 28 of life. on day ... | 2009 | 19237219 |
| clostridium glycolicum wound infections: case reports and review of the literature. | we describe two cases of clostridium glycolicum wound infections in immunocompetent adults. the bacterium was identified by 16s rrna gene sequencing. this is the third published report of the recovery of this organism from human clinical material and highlights the importance of the organism as a potential human pathogen. our report extends the spectrum of the diseases caused by c. glycolicum. | 2009 | 19261780 |
| molecular inventory of faecal microflora in patients with crohn's disease. | intestinal microbial community is involved in the pathogenesis of crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. using sequence analysis of randomly cloned bacterial 16s ribosomal dna, the dominant faecal species from four crohn's disease patients and four controls were compared. whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly re ... | 2004 | 19712374 |
| trophic links between the acetogen clostridium glycolicum kha and the fermentative anaerobe bacteroides xylanolyticus khb isolated from hawaiian forest soil. | isolate kh was obtained from forest soil and found to be composed of two functionally linked anaerobes, kha and khb. gene analyses (16s rrna, fhs, coos) identified kha as an acetogenic strain of clostridium glycolicum and khb as bacteroides xylanolyticus. khb fermented xylan and other saccharides that kha could not utilize and formed products (e.g., ethanol and h(2)) that supported the acetogenic growth of kha. | 2011 | 21764978 |
| effects of a simple or a complex starter microbiota on intestinal microbiota composition in caesarean derived piglets. | the present study was designed to develop a model in piglets that allows the investigation of the effects of postnatal association with a simple or a complex microbiota on gut health and development. thirty piglets from 2 sows were obtained by caesarean delivery (day 0) and were equally divided over 2 treatment groups housed in separate clean, nonsterile rooms. all piglets received orally a simple microbiota consisting of lactobacillus amylovorus, clostridium glycolicum, and parabacteroides spp. ... | 2012 | 23365401 |
| detection of pathogenic clostridia in biogas plant wastes. | as the number of biogas plants has grown rapidly in the last decade, the amount of potentially contaminated wastes with pathogenic clostridium spp. has increased as well. this study reports the results from examining 203 biogas plant wastes (bgws). the following clostridium spp. with different frequencies could be isolated via a new enrichment medium (krüne medium) and detected by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (maldi-tof ms): clostridium perfringens ... | 2015 | 24984829 |
| characterization of romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus clostridium into the genera romboutsia gen. nov., intestinibacter gen. nov., terrisporobacter gen. nov. and asaccharospora gen. nov. | a gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated cribt, was isolated from the gastro-intestinal tract of a rat and characterized. the major cellular fatty acids of strain cribt were saturated and unsaturated straight-chain c12-c19 fatty acids, with c16:0 being the predominant fatty acid. the polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. the ... | 2014 | 24480908 |
| [bioconversion of cellulose to methane by a consortium consisting of four microbial strains]. | cellulose was usually degraded by microbial communities in natural habitats. construction of a simple cellulolytic consortium is necessary to understand the underlying interaction within microorganisms involved in cellulose conversion. a screening approach was developed to obtain a simple microbial community with the ability of cellulose degradation to methane. this technique was based on the method of enrichment culture accompanying with denaturing gel gradient electrophoresis (dgge) fingerprin ... | 2014 | 24720223 |
| a case report of a deep surgical site infection with terrisporobacter glycolicus/t. mayombei and review of the literature. | there are increasing data regarding terrisporobacter glycolicus as an emerging anaerobic pathogen. however, the few published cases to date usually report it as part of a polymicrobial infection. here, we describe the first reported monomicrobial surgical site infection with this bacterium. identification methods, taxonomy, and clinical management of this rarely identified pathogen are also discussed. | 2016 | 27686579 |
| terrisporobacter petrolearius sp. nov., isolated from an oilfield petroleum reservoir. | a gram-staining-positive, spore-forming, strictly anaerobic bacterium, designated strain lam0a37t, was isolated from enrichment samples collected from a petroleum reservoir in shengli oilfield. cells of strain lam0a37t were rod-shaped and motile by peritrichous flagella. the optimal temperature and ph for growth were 40 °c and 7.0–7.5, respectively. the strain did not require nacl for growth but tolerated up to 3 % (w/v) nacl. strain lam0a37t was able to utilize glucose, fructose, maltose, xylos ... | 2015 | 26297478 |