the effect of fixation procedures on the electron density of polysaccharide granules in nocardia corallina. 197551094
[distribution of pyrimidine blocks in the dna of brevibacterium linens, arthrobacter globiformis, nocardia corallina and nocardia rubra].the nucleotide composition and the frequency of pyrimidine blocks were studied in dna of the following bacteria: brevibacterium linens (weignamm, 1910) breed, 1953; arthrobacter globiformis (conn, 1928) conn et dimmick, 1947; nocardia corallina (bergey et al., 1923) waksman et henrici, 1948; nocardia rubra (krassilnikov, 1949) waksman et henrici, 1948. these organisms are classed by some microbiologists as mycobacteria (the mycobacteriaceae family) while other authors regard them as representati ...1975241002
[nocardia corallina pigment]. 19761026746
effect of growth substrates on morphology of nocardia corallina.cells of nocardia corallina atcc 4273 form multiply branched coenocytic mycelia and subsequent fragment to spherical cells when grown on solidified complex media. in liquid shake cultures using complex media the organisms grow into pleomorphic but seldomly branched rods, divide as rods and then the rods fragment to spheres as the stationary phase is reached. in a defined liquid medium with glucose as carbon source, the organisms divide entively as spheres at a doubling time of 44 hrs. the additi ...19751147739
mutualistic degradation of the lignin model compound veratrylglycerol-beta-(o-methoxyphenyl) ether by bacteria.complete degradation of the lignin model compound veratrylglycerol-beta-(o-methoxyphenyl) ether is accomplished mutualistically by a two-membered bacterial culture. bacterial isolate e1, which has been tentatively identified as an acinetobacter, grows on veratrylglycerol-beta-(o-methoxyphenyl) ether producing guaiacol (o-methoxyphenol) as a non-metabolizable, bacteriocidal by-product. when nocardia corallina (strain a81) is also present in media containing veratrylglycerol-beta-(o-methoxyphenyl) ...19751201511
characterization of rrh4273i, a restriction-modification system of rhodococcus rhodochrous atcc 4273 (nocardia corallina) which recognizes the same sequence as the streptomyces albus g sali restriction-modification system.rhodococcus rhodochrous atcc 4275 (nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the sali restriction-modification system. both the restriction endonuclease and the dna-methyltransferase (dna-mtase) have been partially purified and characterized. the nuclease has requirements of activity similar to sali, and a native mr of about 46,000. the dna-mtase is a protein with an mr of about 67,000. no dna homology was ...19911919505
[ultrastructural features of rhodococcus rubropertinctus and streptococcus lactis dissociants].three rhodococcus rubropertinctus dissociants (r, s and m) and two streptococcus lactis dissociants (r and s) were compared using the electron microscopy method of negative contrasting. the cell wall of r.rubropertinctus dissociants had a thickness of 40 nm (r), 30 nm (s) and 20 nm (m). the cell wall of s. lactis dissociants was 35-55 nm (r) and 25-30 nm (s) thick. 1.5-2-fold variations in the thickness of cell walls could account for the different resistance of the dissociants against the actio ...19911921769
steroid transhydrogenase activity of 3-ketosteroid-delta 1-dehydrogenase from nocardia corallina.3-ketosteroid-delta 1-dehydrogenase from nocardia corallina catalyzes transhydrogenation of 3-keto-4-ene-steroid to 3-keto-1,4-diene-steroid e.g., progesterone to 1,4-androstadiene-3,17-dione. the reaction proceeded linearly at first and then soon slowed down owing to equilibration. the turnover number of this reaction was of the same magnitude as that of the dehydrogenation of 3-keto-4-ene-steroid. the ph optimum was 8.4, which is lower than that of the dehydrogenase reaction. the enzyme has a ...19902229003
microbial metabolism studies of the antimalarial sesquiterpene artemisinin.microbial metabolism of the sesquiterpene lactone antimalarial drug artemisinin [1] was studied. screening studies have shown a number of microorganisms capable of metabolizing artemisinin [1]. scale-up fermentation with nocardia corallina (atcc 19070) and penicillium chrysogenum (atcc 9480) have resulted in the production of two major microbial metabolites that have been characterized with the use of 2d-nmr techniques. these metabolites have been identified as deoxyartemisinin [2] and 3 alpha-h ...19892746260
metabolism of endogenous trehalose by streptomyces griseus spores and by spores or cells of other actinomycetes.the disaccharide trehalose is accumulated as a storage product by spores of streptomyces griseus. nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. in contrast, spores rapidly depleted their trehalose pools during the first hours of germination. extracts of dormant spores contained a high specific activity of the enzyme trehalase. the level of trehalase remained relatively constant during germination or incubation in buffer. nongerminating sp ...19873117770
nocardioform actinomycete (rhodococcus rubropertinctus)-induced abortion in a mare. 19883212898
bacterial metabolism of side chain fluorinated aromatics: cometabolism of 3-trifluoromethyl(tfm)-benzoate by pseudomonas putida (arvilla) mt-2 and rhodococcus rubropertinctus n657.the tol plasmid-encoded enzymes of the methylbenzoate pathway in pseudomonas putida mt-2 cometabolized 3-trifluoromethyl (tfm)-benzoate. two products, 3-tfm-1,2-dihydroxy-2-hydrobenzoate (3-tfm-dhb) and 2-hydroxy-6-oxo-7,7,7-trifluoro-hepta-2,4-dienoate (7-tfhod) were identified chemically and by spectroscopic properties. tfm-substituted analogues of the metabolites of the methylbenzoate pathway were generally converted at drastically reduced rates. the catechol-2,3-dioxygenase from pseudomonas ...19883365096
[regulation of terephthalate catabolism in rhodococcus rubropertinctus].the regulation of terephthalate catabolism was studied in rhodococcus rubropertinctus which decomposed this synthetic monomer. the pathway (a) of terephthalate (tp) catabolism is as follows: tp----benzoate----4-hydroxybenzoate----protocatechuate----pyrocatechol-- --cycle ortho-cleavage. the following results were obtained when studying why two other catabolic pathways were realized if benzoate and 4-hydroxybenzoate were taken as a sole carbon source, namely, (b) benzoate----pyrocatechol----cycle ...19863821594
sulindac oxidation/reduction by microbial cultures; microbial models of mammalian metabolism.the oxidation and reduction of the sulphoxide moiety of the anti-inflammatory agent sulindac was investigated to explore microbial systems exhibiting parallels of known mammalian metabolism. of 24 cultures initially screened, four catalysed the expected reactions in analytical studies. arthrobacter species (atcc 19140) and sporobolomyces pararoseus (atcc 11386) produced sulindac sulphide, aspergillus alliaceus (nrrl 315) produced sulindac sulphone, and nocardia corallina (atcc 19070) produced bo ...19854072250
[in vitro study of characteristics of 5 alpha-delta 4 steroid reductase from nocardia corallina]. 19724144963
evidence for the pentose cycle in nocardia corallina. 19664380627
glutamine synthetase from nocardia corallina. 19714396340
[demonstration of a 5 - 4-steroid reductase in cell-free extracts of nocardia corallina]. 19724622360
[effect of thiamine on pigment formation by mycobacterium luteum and nocardia corallina]. 19734789637
the mycolic acids of mycobacterium rhodochrous and nocardia corallina. 19744854962
gas chromatography and mass spectrometry of the trimethylsilyl ether methyl ester derivatives of long chain hydroxy acids from nocardia corallina. 19715113498
pigment of nocardia corallina. 19665958117
enzyme and permeability changes during morphogenesis of nocardia corallina. 19676045660
numerical classification of some rhodococci, corynebacteria and related organisms.nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ...19826811696
microbial transformations of warfarin: stereoselective reduction by nocardia corallina and arthrobacter species.the microbiological metabolism of warfarin was examined as a model of metabolism in higher organisms, including humans, and to determine the chirality of microbial reductases for application in organic synthesis. nineteen cultures were examined based on their reported abilities to reduce ketonic substrates, and several were shown to catalyze the desired reaction. nocardia corallina (atcc 19070) exhibited complete substrate and product stereoselectivity as it reduced s-warfarin to the correspondi ...19827081986
cloning of the nocardia corallina polyhydroxyalkanoate synthase gene and production of poly-(3-hydroxybutyrate-co-3-hydroxyhexanoate) and poly-(3-hydroxyvalerate-co-3-hydroxyheptanoate).the polyhydroxyalkanoate (pha) synthase gene (phacnc) from nocardia corallina was identified in a lambda library on a 6-kb bamhi fragment. a 2.8-kb xhoii subfragment was found to contain the intact pha synthase. this 2.8-kb fragment was subjected to dna sequencing and was found to contain the coding region for the pha synthase and a small downstream open reading frame of unknown function. on the basis of dna sequence, phacnc is closest in homology to the pha synthases (phacpai and phacpaii) of p ...19989783428
heterologous expression of alkene monooxygenase from rhodococcus rhodochrous b-276.alkene monooxygenase (amo) from rhodococcus rhodochrous (formerly nocardia corallina) b-276 is a three-component enzyme system encoded by the four-gene operon amoabcd. amo catalyses the stereoselective epoxygenation of aliphatic alkenes, yielding primarily r enantiomers. the presumed site of alkene oxygenation is a dinuclear iron centre similar to that in the soluble methane monooxygenases of methanotrophic bacteria, to which amo exhibits a significant degree of amino acid sequence identity. the ...199910095780
degradation of methoxylated benzoic acids by a nocardia from a lignin-rich environment: significance to lignin degradation and effect of chloro substituents.strain a81 of nocardia corallina hydroxylates or demethylates p-anisic acid to p-hydroxybenzoic acid and isovanillic acid. it demethylates veratric acid to a mixture of vanillic and isovanillic acids. these are both demethylated to protocatechuic acid, which undergoes ring cleavage to afford beta-carboxy-cis-cis-muconic acid. the intermediacy of protocatechuic acid in the catabolic pathway of veratric acid was confirmed by blocking ring cleavage with an additional substituent in the ring: 5-chlo ...19734743871
gordonia desulfuricans sp. nov., a benzothiophene-desulphurizing actinomycete.the taxonomic position of two actinomycetes isolated from soil was established using a polyphasic approach. the organisms, designated 213et and 213f, were found to have chemical and morphological properties consistent with their assignment to the genus gordonia. nearly complete sequences of the 16s rdna genes of the two strains were determined following the isolation and direct sequencing of the amplified genes. the tested strains were found to have identical 16s rdna sequences and formed a phyl ...199910555368
identification of a lipoarabinomannan-like lipoglycan in gordonia rubropertincta.lipoarabinomannans are well characterised lipoglycans present in the cell envelopes of mycobacteria and closely related bacteria. to define further the distribution of these lipoglycans we have investigated the mycolic acid-containing bacterium gordonia rubropertincta and, by analysis of carbohydrate composition and antigenic cross-reactivity, demonstrated the presence of a lipoarabinomannan-like lipoglycan. a fraction that appeared to contain higher phosphatidylinositolmannosides was also isola ...199910794140
gordonia nitida sp. nov., a bacterium that degrades 3-ethylpyridine and 3-methylpyridine.a bacterial strain, le31t, which is capable of degrading 3-ethylpyridine and 3-methylpyridine, was isolated from an industrial wastewater and was taxonomically studied by using a polyphasic approach. strain le31t was identified as a member of the genus gordonia on the basis of chemotaxonomic characteristics and phylogenetic inference-based 16s rdna sequence. the cell wall contained meso-diaminopimelic acid, arabinose and galactose (wall chemotype iv). the predominant menaquinone was mk-9(h2). th ...200010843064
characterization of s-triazine herbicide metabolism by a nocardioides sp. isolated from agricultural soils.atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. nine gram-positive bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from four farms in central canada. the strains were divided into two groups based on repetitive extragenic palindromic (rep)-pcr genomic fingerprinting with eric and boxa1r primers. based on 16s ribosomal dna sequence analysis, both groups were identified as nocardioides sp. strains. none ...200010919761
gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete.the taxonomic position of a dibenzothiophene-desulphurizing soil actinomycete was established using a polyphasic taxonomic approach. the organism, strain iegmt, was shown to have chemical and morphological properties typical of members of the genus gordonia. the tested strain formed a distinct phyletic line within the evolutionary radiation occupied by the genus gordonia, with gordonia alkanivorans dsm 44369t, gordonia desulfuricans ncimb 40816t and gordonia rubropertincta dsm 43197t as the most ...200011155977
benzo[b]thiophene desulfurization by gordonia rubropertinctus strain t08.a benzothiophene-desulfurizing bacterium which has a novel desulfurization pathway was isolated and identified as gordonia rubropertinctus strain t08. gas chromatography/mass spectroscopy analysis of the ethyl acetate extract of the culture broth detected benzothiophene sulfoxide, benzothiophene sulfone, benzo[e][1,2]oxathiin s-oxide (bt-sultine), benzo[e][1,2]oxathiin s,s-dioxide (bt-sultone), o-hydroxystyrene, and 2-coumaranone, but not 2-(2'-hydroxyphenyl)ethan-1-al, which has been reported t ...200111693923
gordonia namibiensis sp. nov., a novel nitrile metabolising actinomycete recovered from an african sand.a polyphasic approach was used to establish the taxonomic position of two actinomycetes isolated from a namibian soil and shown to utilise nitrile compounds as growth substrates. the organisms, strains nam-bn063at and nam-bn063b, had chemical and morphological properties consistent with their assignment to the genus gordonia. direct 165 rrna sequencing studies confirmed the taxonomic position of the strains following the generation of phylogenetic trees using four different algorithms. the strai ...200111876358
[comparison of the sensitivity of rhodococcus rubropertinctus r, s and m variants to antibiotic action].comparison of sensitivity of 63 clones of r, s and m variants of rhodococcus rubropertinctus 104 to 6 antibiotics showed that sensitivity of the variants was similar only to erythromycin. their sensitivity to penicillin, streptomycin, chlortetracycline, rifampicin and actinomycin c was different. still, they preserved similar regularity: m cells were the most sensitive and r cells were the most resistant. there was a 1.5-3 fold difference in sensitivity of r and m variants to the antibiotics. th ...19873674838
microbiological and chemical transformations of argentatin b.argentatin b is a naturally occurring tetracyclic triterpene isolated from parthenium argentatum x p. tomentosa. it was microbiologically transformed to 16, 24-epoxycycloartan-3alpha, 25-diol, (isoargentatin d), by nocardia corallina var. taoka atcc 31338, mycobacterium species nrrl b3683 and septomyxa affinis atcc 6737. the later microbe also produced 16, 24-epoxycycloartan-3beta, 25-diol (argentatin d) and 1, 2-didehydroargentatin b, (isoargentatin d). sodium hydroxide converted argentatin b t ...200312710737
lung infection caused by rhodococcus.we report a case of lung infection, clinically resembling tuberculosis, caused by rhodococcus rubropertinctus. the patient had no apparent immunosuppression which is unusual for disease caused by the 'rhodochrous' complex. the infection responded successfully to oral anti-tuberculous therapy, which included rifampicin, and to oral tetracycline.19883242467
a cytological study of nocardia corallina and other actinomycetes. 195413152065
effect of successive x-irradiation on nocardia corallina. 195713462956
cytogenetic study of nocardia corallina. 195713462957
studies on the nutrition of nocardia corallina. 195713475225
oxidation of propionic acid by nocardia corallina. 195713475250
slime as a possible factor in cell clumping in nocardia corallina. 195813525342
variation to radiation resistance in nocardia corallina. 195913641204
decomposition of pyrimidines by nocardia corallina. 196113687767
induction of enzymes for pyrimidine catabolism in nocardia corallina. 196113687768
photoprotection against x-ray inactivation in nocardia corallina. 196113693833
heterotrophic fixation of carbon dioxide by extracts of nocardia corallina. 196013797685
fat bodies in nocardia corallina. 196013810456
overlap of site of action of x-ray and ultraviolet irradiation in nocardia corallina. 196013810457
propionate carboxylation by nocardia corallina. 196113865894
oxidation of polyols by nocardia corallina.maurer, p. r. (university of otago medical school, dunedin, new zealand) and r. d. batt. oxidation of polyols by nocardia corallina. j. bacteriol. 83:1131-1139. 1962.-two diphosphopyridine nucleotide-linked polyol dehydrogenases were induced in nocardia corallina strain s, and grown on either mannitol or sorbitol. one enzyme was labile and was specific for the conversion of sorbitol to d-fructose. the second enzyme, which converted d-mannitol to d-fructose and, more slowly, d-arabitol to d-xylul ...196214471555
evolution of the soluble diiron monooxygenases.based on structural, biochemical, and genetic data, the soluble diiron monooxygenases can be divided into four groups: the soluble methane monooxygenases, the amo alkene monooxygenase of rhodococcus corallinus b-276, the phenol hydroxylases, and the four-component alkene/aromatic monooxygenases. the limited phylogenetic distribution of these enzymes among bacteria, together with available genetic evidence, indicates that they have been spread largely through horizontal gene transfer. phylogeneti ...200314550940
purification and characterisation of glutamine synthetase from nocardia corallina.glutamine synthetase (gs) (ec has been purified 67-fold from nocardia corallina. the apparent mr of the gs subunit was approximately 56,000. assuming the enzyme is a typical dodecamer this indicates a particle mass for the undissociated enzyme of 672,000. the gs is regulated by adenylylation and deadenylylation, and subject to feedback inhibition by alanine and glycine. the ph profiles assayed by the gamma-glutamyl transferase method were similar for nh+4-treated and untreated cell extr ...19882906794
spectral properties of 3-ketosteroid-delta 1-dehydrogenase from nocardia corallina.3-ketosteroid-delta 1-dehydrogenase from nocardia corallina is a flavoenzyme that catalyzes 1,2-desaturation of 3-ketosteroid. the dehydrogenase generated complexes with 3-ketosteroids and phenolic steroids such as estradiol with remarkable perturbations of the visible spectrum. the enzyme did not make the adduct with sulfite ion, but could use molecular oxygen as the electron acceptor. the cd spectra of oxidized and steroid-bound enzymes exhibited positive dichroisms in the visible region which ...19902400777
purification and characterization of 3-ketosteroid-delta 1-dehydrogenase from nocardia corallina.the inducible 3-ketosteroid-delta 1-dehydrogenase of nocardia corallina which catalyzes the introduction of a double bond into the position of carbon 1 and 2 of ring a of 3-ketosteroid has been obtained in four steps with a 50% yield and 360-fold purification. the enzyme is homogeneous as judged by sds-gel electrophoresis and is a monomeric protein with a molecular weight of 60,500. the isoelectric point of the enzyme is about 3.1. the enzyme contains 1 mol of flavin adenine dinucleotide per mol ...19902317517
microbial metabolism studies of the antimalarial drug arteether.microbial metabolism studies of the antimalarial drug arteether (1) have shown that arteether is metabolized by a number of microorganisms. large-scale fermentation with aspergillus niger (atcc 10549) and nocardia corallina (atcc 19070) have resulted in the isolation of four microbial metabolites which have been characterized using two-dimensional nuclear magnetic resonance (2d-nmr) techniques. these metabolites have been identified as "aem1" (2), 3 alpha-hydroxydeoxyarteether (3), 3 alpha-hydro ...19902308900
3-keto-5 alpha-steroid-delta 4-dehydrogenase from nocardia corallina: purification and characterization.the inducible 3-keto-5 alpha-steroid-delta 4-dehydrogenase of nocardia corallina was purified to homogeneity using affinity chromatography on 19-nortestosterone-17-acetoxyaminoethyl sepharose 4b. sds-polyacrylamide gel electrophoresis, gel filtration and spectral analysis of flavin suggest that the purified dehydrogenase is a monomeric protein of mr 60,000 containing one flavin. it has a typical absorption spectrum of flavoprotein with maxima at 457, 375, and 277 nm. the values shifted to 470 an ...19911869511
secondary life cycle of nocardia corallina. 195716561831
endogenous respiration and oxidative assimilation in nocardia corallina. 196016561850
essential histidine residue in 3-ketosteroid-delta 1-dehydrogenase.the variation with ph of kinetic parameters was examined for 3-ketosteroid-delta 1-dehydrogenase from nocardia corallina. the vmax/km profile for 4-androstenedione indicates that activity is lost upon protonation of a cationic acid-type group with a pk value of 7.7. the enzyme was inactivated by diethylpyrocarbonate at ph 7.4 and the inactivation was substantially prevented by androstadienedione. analyses of reactivation with neutral hydroxylamine, ph variation, and spectral changes of the inact ...19921639754
silicone-immobilized biocatalysts effective for bioconversions in nonaqueous media.a hydrophobic silicone polymer could be effectively applied to immobilization of two kinds of biocatalysts operating in organic media. horse liver alcohol dehydrogenase, which was solubilized in a small amount of water, or deposited on water-filled hydrophilic particles, was immobilized in this material. this configuration of the preparation involving finely dispersed aqueous phase permitted a simple packed-bed operation for the enzymatic oxidation of alcohol and reduction of aldehyde with a cou ...19921368113
[destruction of motor oils by actinobacteria].the influence of high concentrations of motor oils (mo) and additives to them on the growth of strains dietzia maris ukm ac-205, gordonia rubripertincta ukm ac-179 and rhodococcus erythropolis ukm ac-50 as well as the ability of these actinobacteria to destruction of different mo brands were investigated. it was shown that all strains were resistant to the used motor oils but showed sensitivity to fresh (unused) mo and additives containing zinc dithiophosphate. the oil "esso ultra" which had min ...201020812505
immobilization of microbial cells in a mixed matrix of silicone polymer and calcium alginate gel: epoxidation of 1-octene by nocardia corallina b-276 in organic media.immobilization of nocardia corallina b-276 was examined for production of 1,2-epoxyoctane from 1-octene in the presence of n-hexadecane as the organic solvent. hydrophobic silicone polymer was the most suitable material for entrapment of the cells. coentrapment of aqueous reaction medium into the silicone polymer matrix improved the epoxide productivity. it was also effective to immobilize the cells in a mixed matrix composed of silicone polymer and calcium alginate gel involving the reaction me ...19901366873
[significance of evaluation of the antibiotic-resistant properties of bacterial producing strains in the hygienic standardization system].the study was undertaken to evaluate the antibiotic sensitivity of microorganisms that were used in biotechnological production and belonged to different taxons: gram-positive (bacillus subtilis, bacillus licheniformis, and bacillus thuringiensis) and gram-negative (alcaligenes denitrificans and pseudomonas caryophylii) bacteria and noncardioform actinomycetes (rhodococcus erythropolis and rhodococcus corallinus). the sensitivity of the strains to a range of antibiotics was determined by the aga ...201021344698
[the effect of physicochemical environmental factors on the growth of gram-positive bacterial dissociants].the growth of r-, s- and m (g)-dissociants of streptococcus lactis, bacillus coagulans, rhodococcus rubropertinctus under the action of some physico-chemical factors: temperature, ph, ultraviolet (uv) rays, high concentration of nacl and storage have been compared. r-variants gain selective advantage under the influence of uv-irradiation, high temperature and storage; s-variants--at decreasing of active ph of medium; m (g)-variants--at decreasing of growth temperature, high values of ph, increas ...19921302518
indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance.introduction: transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. the purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora. methods: microorganisms in pristine and oily soils wer ...201121948132
[nocardia pellegrini: iii. an attempt of phage typing(author's transl)].the typing of nocardia pellegrini with different nocardia-phages was studied. the investigation included 25 nocardia pellegrini strains, 19 strains of different nocardia species and also strains of different species of mycobacteria. it could be stated that phages typical for nocardia pellegrini were also active against other fast fragmentating nocardia species, such as nocardia corallina or nocardia rubra. however, they did not attack nocardia asteroides or strains of mycobacteria. phages typica ...19751098326
[fine structure of nocardia corallina oxidizing phenol].intracellular structures were detected in nocardia corallina, strain 4, on a medium containing phenol as a sole source of carbon but not on a conventional growth medium (mpa). the structures are light vacuoles containing granular and fibrillar electron-dense substance. the structures specific of this medium may be regions of phenol oxidation.19761004250
degradation of trichloroethene by a linear-plasmid-encoded alkene monooxygenase in rhodococcus corallinus (nocardia corallina) b-276.rhodococcus corallinus (formerly nocardia corallina) b-276, isolated with propene as sole carbon and energy source, is able to oxidize trichloroethene (tce). glucose- or propene-grown r. corallinus b-276 cells exhibited no difference in tce degradation efficiency. tce degradation was found to be growth-phase-dependent and maximum rates were monitored with stationary-phase cells. k(m) and vmax values for tce degradation of r. corallinus b-276 grown in nutrient broth medium in the presence of gluc ...199910439411
evidence for an inducible nucleotide-dependent acetone carboxylase in rhodococcus rhodochrous b276.the metabolism of acetone was investigated in the actinomycete rhodococcus rhodochrous (formerly nocardia corallina) b276. suspensions of acetone- and isopropanol-grown r. rhodochrous readily metabolized acetone. in contrast, r. rhodochrous cells cultured with glucose as the carbon source lacked the ability to metabolize acetone at the onset of the assay but gained the ability to do so in a time-dependent fashion. chloramphenicol and rifampin prevented the time-dependent increase in this activit ...199910217764
electron transfer reactions in the alkene mono-oxygenase complex from nocardia corallina b-276.nocardia corallina b-276 possesses a multi-component enzyme, alkene mono-oxygenase (amo), that catalyses the stereoselective epoxygenation of alkenes. the reductase component of this system has been shown by epr and fluorescence spectroscopy to contain two prosthetic groups, an fad centre and a [2fe-2s] cluster. the role of these centres in the epoxygenation reaction was determined by midpoint potential measurements and electron transfer kinetics. the order of potentials of the prosthetic groups ...199910085230
sequence-alignment modelling and molecular docking studies of the epoxygenase component of alkene monooxygenase from nocardia corallina b-276.whole cells of nocardia corallina b-276 catalyse the stereoselective epoxygenation of alkenes to chiral epoxides. the bacterium expresses an enzyme, alkene monooxygenase, which catalyses the epoxygenation reaction stereoselectively. the enzyme consists of a terminal oxygenase (epoxygenase), an nadh-dependent reductase (reductase) and a regulatory component (coupling protein). the epoxygenase component contains a bridged diiron centre similar to that found in the hydroxylase component of soluble ...19989688257
identification and characterization of epoxide carboxylase activity in cell extracts of nocardia corallina b276.the metabolism of aliphatic epoxides (epoxyalkanes) by the alkene-utilizing actinomycete nocardia corallina b276 was investigated. suspensions of n. corallina cells grown with propylene as the carbon source readily degraded propylene and epoxypropane, while suspensions of glucose-grown cells did not. the addition of propylene and epoxypropane to glucose-grown cells resulted in a time-dependent increase in propylene- and epoxypropane-degrading activities that was prevented by the addition of rifa ...19989555888
alkene monooxygenase from nocardia corallina b-276 is a member of the class of dinuclear iron proteins capable of stereospecific epoxygenation reactions.nocardia corallina b-276 possesses a constitutive multi-component alkene monooxygenase which catalyses the epoxidation of terminal and sub-terminal alkenes. the epoxygenase component of this system has been purified with an overall yield of 35%. the electron paramagnetic resonance spectrum of the oxidised protein has a weak signal at g = 4.3, which we ascribe to rhombic iron and a free radical signal at g(ave) = 2.01. upon partial reduction with dithionite using methyl viologen as a mediator, a ...19979266707
application of an optimized electroporation procedure for replacement of the polyhydroxyalkanoate synthase i gene in nocardia develop a system for gene replacement in nocardia corallina, a protocol for electroporation was optimized by systematic alterations of growth conditions, field strength, time constant and the electroporation buffer. transformation efficiencies of 0.5 x 10(6) - 3 x 10(6) transformants/microgram plasmid dna were obtained routinely. the gene encoding the polyhydroxyalkanoate (pha) synthase i of n. corallina was cloned and interrupted by insertion of a kanamycin-resistance gene. the resulting pla ...19968764686
atrazine chlorohydrolase from pseudomonas sp. strain adp: gene sequence, enzyme purification, and protein characterization.pseudomonas sp. strain adp metabolizes atrazine to carbon dioxide and ammonia via the intermediate hydroxyatrazine. the genetic potential to produce hydroxyatrazine was previously attributed to a 1.9-kb avai dna fragment from strain adp (m. l. de souza, l. p. wackett, k. l. boundy-mills, r. t. mandelbaum, and m. j. sadowsky, appl. environ. microbiol. 61:3373-3378, 1995). in this study, sequence analysis of the 1.9-kb avai fragment indicated that a single open reading frame, atza, encoded an acti ...19968759853
metabolic pathway for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) formation in nocardia corallina: inactivation of mutb by chromosomal integration of a kanamycin resistance gene.the gene encoding the large subunit of the methylmalonyl-coenzyme a (coa) mutase in nocardia corallina (mutbnc) was cloned. a 4.3-kbp bamhi fragment containing almost the entire mutbnc was identified by southern hybridization experiments employing a digoxigenin-labeled probe deduced from mutb of streptomyces cinnamonensis, mutbnc was interrupted by insertion of a kanamycin resistance gene block (mutb::kan or mutb::neo) and introduced into n. corallina to obtain mutb-negative strains by homologou ...19968593043
cloning and expression of the s-triazine hydrolase gene (trza) from rhodococcus corallinus and development of rhodococcus recombinant strains capable of dealkylating and dechlorinating the herbicide atrazine.we used degenerate oligodeoxyribonucleotides derived from the n-terminal sequence of the s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r in an amplification reaction to isolate a dna segment containing a 57-bp fragment from the trza gene. by using the nucleotide sequence of this fragment, a nondegenerate oligodeoxyribonucleotide was synthesized and used to screen a genomic library of r. corallinus dna for fragments containing trza. a 5.3-kb psti fragment containing trza was cloned ...19957592318
synthesis of imidazol-2-yl amino acids by using cells from alkane-oxidizing bacteria.sixty-one strains of alkane-oxidizing bacteria were tested for their ability to oxidize n-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide to imidazol-2-yl amino acids applicable for pharmaceutical purposes. after growth with n-alkane, 15 strains formed different imidazol-2-yl amino acids identified by chemical structure analysis (mass and nuclear magnetic resonance spectrometry). high yields of imidazol-2-yl amino acids were produced by the strains gordonia rubropertincta sbug 105, gordonia terra ...200312620858
effect of starvation on resuscitation and the surface characteristics of bacteria.resuscitation behavior of bacteria after starvation for carbon and nitrogen was investigated. in addition effect of carbon and nitrogen starvation conditions on the surface characteristics and adhesive properties of bacteria were studied. two pure culture herbicide degrading bacteria were used in the study: pseudomonas sp. strain a, and rhodococcus corallinus strain 11. these bacteria are known to degrade cyanuric acid which is a derivative of s-triazine, a common herbicide used widely. selected ...200312929805
rapid detection and identification of the metabolically diverse genus gordonia by 16s rrna-gene-targeted genus-specific primers.the importance of the emerging genus gordonia in industrial and environmental biotechnology is evidenced by the recent increase in associated publications and patents. but, investigations into potentially valuable gordonia members are restricted by the limitations of current isolation and detection techniques. this motivated us to design a genus-specific oligonucleotide primer pair which could assist in rapid detection of species of the genus gordonia by means of pcr-specific amplification. the ...200516098691
purification and characterization of an inducible s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r.the widespread use and relative persistence of s-triazine compounds such as atrazine and simazine have led to increasing concern about environmental contamination by these compounds. few microbial isolates capable of transforming substituted s-triazines have been identified. rhodococcus corallinus nrrl b-15444 has previously been shown to possess a hydrolase activity that is responsible for the dechlorination of the triazine compounds deethylsimazine (6-chloro-n-ethyl-1,3,5-triazine-2,4-diamine) ...199416349190
two short-chain dehydrogenases confer stereoselectivity for enantiomers of epoxypropane in the multiprotein epoxide carboxylating systems of xanthobacter strain py2 and nocardia corallina b276.epoxide carboxylase from the bacterium xanthobacter strain py2 is a multicomponent enzyme system which catalyzes the pyridine nucleotide-dependent carboxylation of aliphatic epoxides to beta-ketoacids as illustrated by the reaction epoxypropane + co2 + nadph + nad+ --> acetoacetate + h+ + nadp+ + nadh. the combination of four distinct proteins, designated components i-iv, are required for the reconstitution of epoxide carboxylase activity with racemic mixtures of short-chain (c3-c5) terminal epo ...19999890905
cloning, expression, and site-directed mutagenesis of the propene monooxygenase genes from mycobacterium sp. strain m156.propene monooxygenase has been cloned from mycobacterium sp. strain m156, based on hybridization with the amoabcd genes of rhodococcus corallinus b276. sequencing indicated that the mycobacterial enzyme is a member of the binuclear nonheme iron monooxygenase family and, in gene order and sequence, is most similar to that from r. corallinus b-276. attempts were made to express the pmoabcd operon in escherichia coli and mycobacterium smegmatis mc(2)155. in the former, there appeared to be a proble ...200515812019
adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases.alkene monooxygenase (amo) from rhodococcus rhodochrous (formerly nocardia corallina) b-276 belongs to a family of multicomponent nonheme binuclear iron-centre oxygenases that includes the soluble methane monooxygenases (smmos) found in some methane-oxidizing bacteria. the enzymes catalyse the insertion of oxygen into organic substrates (mostly hydrocarbons) at the expense of o2 and nad(p)h. amo is remarkable in its ability to oxidize low molecular-mass alkenes to their corresponding epoxides wi ...200515943801
microbial hydrocarbon co-oxidation. iii. isolation and characterization of an alpha, alpha'-dimethyl-cis, cis-muconic acid-producing strain of nocardia corallina.a soil isolate identified as a strain of nocardia corallina accumulated alpha, alpha'-dimethyl-cis, cis-muconic acid under co-oxidation conditions employing n-hexadecane for growth and p-xylene as the co-oxidizable substrate. n. corallina v-49 was postulated to have two pathways for the oxidation of p-xylene. one pathway proceeds throughp-benzyl alcohol, p-tolualdehyde, and p-toluic acid to 2, 3-dihydroxy-p-toluic acid, and the other pathway results in ortho ring cleavage of 3, 6-dimethylpyrocat ...196916349849
analysis of the 7.6-kb cryptic plasmid pnc500 from rhodococcus rhodochrous b-276 and construction of rhodococcus-e. coli shuttle vector.four circular cryptic plasmids were detected from propene-degrading rhodococcus rhodochrous (formerly nocardia corallina) b-276 and the smallest 7.6-kb plasmid, named pnc500 was used to construct rhodococcus-e. coli shuttle vector, pnc5403. sequence analysis of pnc500 revealed that the plasmid contains eight potential orfs, namely 1 through 8. the deduced amino acid sequences for orfs 3, 4, 6, and 7 show homology with those of rep a, rep b, dna methyl-transferase (m.xami), and restriction nuclea ...200717043815
formation of propylene oxide by nocardia corallina immobilized in liquid paraffin.nocardia corallina b276 cells were immobilized by emulsification with liquid paraffin and an antifoam agent at room temperature. the immobilized cells were studied for their ability to carry out the formation of propylene oxide from propylene and oxygen. the evaluations were done with the cells in a bubble-type reactor with a continuous gas feed of 5% propylene and 11.6-95% oxygen, with the balance nitrogen. by using liquid paraffin and antifoam, both the epoxidation activity and the stability w ...198618555334
[growth peculiarities of hydrocarbon-oxidizing rhodococcus and pseudomonads dissociates in mono- and mixed cultures].growth of r-, s-, and m-dissociates of rhodococcus rubropertinctus in mixed culture with r-, s-, and m-dissociates of rhodococcus aeruginosa in comparison with rhodococcus monoculture cultivated on mineral nutrient medium with hexadecane has been studied. the amount of cells in the stationary growth phase has increased 10-15 times in comparison with the monoculture, and pseudomonads which dominated in population, in associations of m-dissociate of r. rubropertinctus with any dissociate of r. aer ...201021061599
modeling horizontal gene transfer (hgt) in the gut of the chagas disease vector rhodnius prolixus.abstract: background: paratransgenesis is an approach to reducing arthropod vector competence using genetically modified symbionts. when applied to control of chagas disease, the symbiont bacterium rhodococcus rhodnii, resident in the gut lumen of the triatomine vector rhodnius prolixus (hemiptera: reduviidae), is transformed to export cecropin a, an insect immune peptide. cecropin a is active against trypanosoma cruzi, the causative agent of chagas disease.while proof of concept has been achiev ...201121569540
[catalase activity of hydrocarbon-oxidizing bacteria].the dynamics of catalase activity of the hydrocarbon-oxidizing bacteria cordona terrae, rhodococcus rubropertinctus, and rhodococcus erythropolis during petroleum product destruction has been studied. a direct relationship between decreasing catalase activity of hydrocarbon-oxidizing microorganisms and the intensity of petroleum product destruction has been established experimentally. the revealed dependence allows one to consider the catalase activity of bacteria as an indicator of the initial ...201223330387
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