| microbacterium koreense sp. nov., from sea water in the south sea of korea. | microbacterium strains js53-2t and js53-5 were isolated from sea water in the south sea of korea and subjected to phenotypic, chemotaxonomic and genetic characterization. the cells were found to be gram-positive. these strains contained mk-11 and mk-12 as the main respiratory quinones and anteiso-c15:0, anteiso-c17:0 and iso-c16:0 as the major cellular fatty acids. the dna g+c content was 68 mol%. the cell-wall sugars of the isolates were galactose and xylose, and the diamino acid in the cell-wa ... | 2006 | 16449451 |
| carbohydrate-binding module 74 is a novel starch-binding domain associated with large and multidomain α-amylase enzymes. | microbacterium aurum b8.a is a bacterium that originates from a potato starch-processing plant and employs a gh13 α-amylase (maamya) enzyme that forms pores in potato starch granules. maamya is a large and multi-modular protein that contains a novel domain at its c terminus (domain 2). deletion of domain 2 from maamya did not affect its ability to degrade starch granules but resulted in a strong reduction in granular pore size. here, we separately expressed and purified this domain 2 in escheric ... | 2016 | 27101946 |
| degradation of granular starch by the bacterium microbacterium aurum strain b8.a involves a modular α-amylase enzyme system with fniii and cbm25 domains. | the bacterium microbacterium aurum strain b8.a, originally isolated from a potato plant wastewater facility, is able to degrade different types of starch granules. here we report the characterization of an unusually large, multidomain m. aurum b8.a α-amylase enzyme (maamya). maamya is a 1,417-amino-acid (aa) protein with a predicted molecular mass of 148 kda. sequence analysis of maamya showed that its catalytic core is a family gh13_32 α-amylase with the typical abc domain structure, followed b ... | 2015 | 26187958 |
| characterization of the starch-acting maamyb enzyme from microbacterium aurum b8.a representing the novel subfamily gh13_42 with an unusual, multi-domain organization. | the bacterium microbacterium aurum strain b8.a degrades granular starches, using the multi-domain maamya α-amylase to initiate granule degradation through pore formation. this paper reports the characterization of the m. aurum b8.a maamyb enzyme, a second starch-acting enzyme with multiple fniii and cbm25 domains. maamyb was characterized as an α-glucan 1,4-α-maltohexaosidase with the ability to subsequently hydrolyze maltohexaose to maltose through the release of glucose. maamyb also displays e ... | 2016 | 27808246 |
| enzymatic degradation of granular potato starch by microbacterium aurum strain b8.a. | microbacterium aurum strain b8.a was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. extracellular enzymes hydrolyzing granular starch were detected in the growth medium of m. aurum b8.a, while the type strain m. aurum dsmz 8600 produced very little amylase activity, and hence was unable to degrade granular starch. the strain b8.a extracellular enzyme fraction degraded wheat, tapioca and potato starch ... | 2011 | 21732245 |
| performance and microbial diversity of a membrane bioreactor treating real textile dyeing wastewater. | the textile industry is one of the major industries of taiwan but unfortunately it produces toxic and low biodegradable wastewater. to remedy this problem, this study compared the performance of the membrane bioreactor (mbr) and sequencing batch reactor (sbr) processes for treating real textile dyeing wastewater. the microbial diversity of the mbr process was also identified by a combination of culturing methods and molecular biotechnology. the removal efficiencies of the mbr process for color, ... | 2007 | 17879852 |
| microbacterium deminutum sp. nov., microbacterium pumilum sp. nov. and microbacterium aoyamense sp. nov. | three novel bacterial strains were isolated from a soil sample collected in japan by culture on a gpm agar plate supplemented with superoxide dismutase and catalase. the strains were gram-positive, catalase-positive, non-motile bacteria with l-ornithine as a diagnostic diamino acid of the peptidoglycan. the acyl type of the peptidoglycan was n-glycolyl. the major menaquinones were mk-12, 13 and 14. mycolic acids were not detected. g+c contents of the dna were in the range 69-71 mol%. comparative ... | 2006 | 16957107 |