| virus-like particles associated with banana bunchy top disease contain small single-stranded dna. | virus-like particles were purified from banana plants with banana bunchy top disease. these particles were isometric with a diameter of 18 to 20 nm and a density of 1.28 to 1.30 g/ml in caesium sulphate. associated with these particles were an ssdna of about 1 kb and one major protein of mr 20,100. dsdna was synthesized from nucleic acid extracts from these particles and cloned. one clone, pbt338, hybridized specifically (i) with sap extracts from plants infected with banana bunchy top virus (bb ... | 1991 | 1993864 |
| novel virus-like particles containing circular single-stranded dnas associated with subterranean clover stunt disease. | novel virus-like particles, 17-19 nm in diameter, have been isolated from subterranean clover and pea plants infected with the pathogen of subterranean clover stunt disease (scsd). the structure and genetic organization of these particles suggest that the pathogen of scsd is representative of a new group of plant dna viruses. scs virus-like particles (scsv) are isometric and band as a single component with buoyant densities of 1.24 g/ml in cs2so4 and 1.34 g/ml in cscl. the a260 nm/a280 nm is abo ... | 1988 | 2847424 |
| the genome organization of banana bunchy top virus: analysis of six ssdna components. | we have cloned, sequenced and analysed an additional five circular ssdna components of banana bunchy top virus (bbtv) which we have called components 2, 3, 4, 5 and 6. these components were present in all bbtv infections tested. four of these components (components 3, 4, 5 and 6) had one large open reading frame (orf) in the virion sense located 3' of a stem-loop structure. each orf had a potential tata box and one or two potential polyadenylation signals associated with it and each polyadenylat ... | 1995 | 7782775 |
| sequence analysis of a faba bean necrotic yellows virus dna component containing a putative replicase gene. | faba bean necrotic yellows virus (fbnyv) has a circular ssdna genome possibly consisting of several components of about 1 kb each. the complete nucleotide sequence of one component of fbnyv (fbnyv dna 1) containing a putative replicase gene is presented. this component consists of 1002 nucleotides and, in the virion orientation, contains one large open reading frame (orf1) potentially encoding a 32.3 kda replicase with the ntp-binding motif ggegks. no obvious functions could be assigned to two s ... | 1995 | 7844570 |
| sequence of subterranean clover stunt virus dna: affinities with the geminiviruses. | the nucleotide sequences of seven circular, single-stranded dna components of one isolate of subterranean clover stunt virus (scsv) have been determined. each component, of about 1 kb, appears to encode a single open reading frame in the same sense as the encapsidated dna. notably, the proteins encoded by two scsv components are related. each has a consensus nucleotide binding motif and shares about 40% amino acid identity with the other and with the putative replication proteins of banana bunch ... | 1995 | 7886939 |
| evidence that banana bunchy top virus has a multiple component genome. | a 93 nucleotide sequence was found to be strongly conserved between two ssdna genomic components of banana bunchy top virus (bbtv). two outwardly extending degenerate primers were designed from this sequence and used in a polymerase chain reaction (pcr) with dna extracted from purified bbtv virions. pcr amplified products consisting of at least seven distinct bands all approximately 1 kb and possibly representing full-length bbtv dsdna were resolved. the pcr amplified products were cloned and th ... | 1994 | 7944956 |
| nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. | one dna component of the banana bunchy top virus (bbtv) genome was cloned and sequenced. this component is present as a circular, ssdna in the virions and consists of 1111 nucleotides. it contains one large open reading frame (orf) of 858 nucleotides in the virion sense; this orf encodes a putative replicase based on the presence of a dntp-binding motif (ggegkt). two smaller orfs (249 and 366 nucleotides), in the complementary orientation, could not be assigned any obvious function. neither of t ... | 1993 | 8445361 |
| putative full-length clones of the genomic dna segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. | subterranean clover stunt disease is an economically important aphid-borne virus disease affecting certain pasture and grain legumes in australia. the virus associated with the disease, subterranean clover stunt virus (scsv), was previously found to be representative of a new type of single-stranded dna virus. analysis of the virion dna and restriction mapping of double-stranded cdna synthesized from virion dna suggested that scsv has a segmented genome composed of 3 or 4 different species of ci ... | 1993 | 8460528 |
| sequence of porcine circovirus dna: affinities with plant circoviruses. | the complete nucleotide sequence (1759 nt) of the ssdna genome of porcine circovirus (pcv) was determined from a cloned dsdna replicative form isolated from pcv-infected cells. sequence analysis detected no significant nucleic acid or protein similarity with another animal circovirus, chicken anaemia virus (cav) but, surprisingly, the highest protein similarity was obtained between the product of the largest predicted pcv orf (orf1; encoding a potential protein of 35.7 kda) and a putative protei ... | 1997 | 9010307 |
| analysis of six dna components of the faba bean necrotic yellows virus genome and their structural affinity to related plant virus genomes. | faba bean necrotic yellows virus (fbnyv) has a multicomponent circular ssdna genome. in addition to a previously described genome component (c1) coding for a replicase-associated protein (rep), five further components (c2 to c6) have now been identified. each of the six components is about 1 kb in size, contains one major open reading frame (orf) in the virion sense with a tata box and polyadenylation signal, and has a noncoding region containing a highly conserved sequence possibly forming a st ... | 1997 | 9217049 |
| beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. | circoviruses are a diverse group of animal and plant pathogens with undefined relationships to one another but for their non-geminate, non-enveloped capsids and circular, single-stranded dna genomes. the sequences of the beak and feather disease virus and porcine circovirus genomic dnas are presented and analyzed in the context of the other members of the family. sequence comparisons, inferred phylogenies, and geographic occurrence suggest that the ambisense circoviruses, particularly the beak a ... | 1998 | 9787657 |
| psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. | cloning and sequencing of the circular, single-stranded dna of one isolate of psittacine beak and feather disease virus (bfdv) demonstrate a genome composed of a circular molecule of 1993 nucleotide bases. an analysis of the assembled replicative form demonstrated seven open reading frames (orfs) (three in the virion strand and four in the complementary strand), potentially encoding seven viral proteins of >8.7 kda. high amino acid sequence similarity was demonstrated between a potential 33.3-kd ... | 1998 | 9791035 |
| ten distinct circular ssdna components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome. | four further circular ssdna components (c7-c10), about 1 kb in size and structurally similar to the previously described components (c1-c6) found associated with a syrian (sy) isolate of faba bean necrotic yellows virus (fbnyv), have been identified. similar to c1 and c2, two of the new components (c7 and c9) encode putative replication-associated (rep) proteins of 33.2 and 32.7 kda, respectively, the former of which is 90% identical to the c10 rep protein of milk vetch dwarf virus (mdv). c8 enc ... | 1998 | 9880028 |
| sequences of ten circular ssdna components associated with the milk vetch dwarf virus genome. | milk vetch dwarf virus (mdv) is a member of the proposed genus nanovirus, and its genome is composed of multiple, circular ssdna components of about 1 kb. we have cloned and sequenced ten ssdna components and designated them mdv-c1 to c10. each dna component contains one potential major open reading frame, and contains a putative stem-loop structure in the non-coding region. notably, four components (c1, c2, c3 and c10) encode distinct replication-associated (rep) proteins of 33 kda, which show ... | 1998 | 9880029 |
| similarity and divergence among viruses in the genus furovirus. | nucleotide sequences of rnas 1 and 2 of a japanese strain of soil-borne wheat mosaic virus (sbwmv), the type species of the genus furovirus, and sorghum chlorotic spot virus (scsv) were determined from cloned cdna. the relationship among the japanese and us strains of sbwmv, scsv, oat golden stripe virus (ogsv), and recently proposed chinese wheat mosaic and european wheat mosaic viruses (cwmv and ewmv) were examined at the nucleotide and amino acid levels. pairwise comparisons of genome-encoded ... | 2000 | 10772992 |
| the master rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment. | faba bean necrotic yellows virus (fbynv), milk vetch dwarf virus (mdv), and subterranean clover stunt virus (scsv) are nanoviruses that infect leguminous plants. from mdv- and scsv-infected tissue we identified viral dnas that encode a replication initiator protein (rep), essential for replication of the multiple circular single-stranded dnas of these viruses. these previously undescribed rep proteins of mdv and scsv are strikingly similar in sequence and functionally equivalent to the master re ... | 2000 | 10936099 |
| the 3' non-coding region of a c4 photosynthesis gene increases transgene expression when combined with heterologous promoters. | the me1 gene of the dicot flaveria bidentis encodes nadp malic enzyme, which catalyses the decarboxylation reaction of c4 photosynthesis in bundle sheath cells. we have previously shown that the 3' non-coding region (me1 3') controls quantitative expression of the gene. we wondered whether me1 3' can increase expression when combined with heterologous promoters. we tested a highly expressed, constitutive promoter, the s4 promoter from subterranean clover stunt virus, and a highly expressed, leaf ... | 2001 | 11488479 |
| birth-and-death evolution of protein-coding regions and concerted evolution of non-coding regions in the multi-component genomes of nanoviruses. | genomes of the four plant viruses of the genus nanovirus consist of multiple circular single-stranded dna components, each of which encodes a single protein. protein phylogenies supported the hypothesis that faba bean necrotic yellows virus (fbnyv) and milk vetch disease virus (mdv) are sister taxa; that subterranean clover stunt virus (scsv) branched next; and that banana bunchy top virus (bbtv) is an outgroup to the three other species. the phylogeny of replication (rep) proteins indicate that ... | 2004 | 14715221 |
| transcripts encoding the nanovirus master replication initiator proteins are terminally redundant. | the multicomponent single-stranded dna plant nanoviruses encode unique master replication initiator (rep) proteins. we have mapped the 5' and 3' termini of the corresponding polyadenylated mrnas from faba bean necrotic yellows virus (fbnyv) and subterranean clover stunt virus and found that these are terminally redundant by up to about 160 nt. moreover, the origin of viral dna replication is transcribed into rna that is capable of folding into extended secondary structures. other nanovirus genom ... | 2008 | 18198390 |
| full-length sequence analysis of a distinct isolate of bidens mottle virus infecting sunflower in taiwan. | the full-length genome of a potyvirus, previously known as sunflower chlorotic spot virus isolate sf-1 (scsv-sf-1) which causes novel symptoms on sunflowers (helianthus annuus), was sequenced and analyzed. the genome of scsv-sf-1 is 9,741 nucleotides long, encoding a polyprotein of 3,071 amino acids containing the consensus motifs of potyviruses. sequence comparison revealed that the 3'-terminus of scsv-sf-1 shared over 96% similarities with isolates of bidens mottle virus (bimov). however, scsv ... | 2009 | 19308314 |