| immunocytochemical identification of spore proteins in two microsporidia, with emphasis on extrusion apparatus. | microsporidia can form small spores with a unique invasive apparatus featuring a long polar tube whose extrusion allows entry of infectious sporoplasm into a host cell. the reactivity of mouse polyclonal antibodies raised against sporal proteins from two microsporidian species belonging to different genera (glugea atherinae and encephalitozoon cuniculi) was studied by western blotting and indirect immunofluorescence. whole protein antisera provided a few cross-reactions relatable to some protein ... | 1998 | 9561775 |
| lipids of three microsporidian species and multivariate analysis of the host-parasite relationship. | sporal lipids of 3 microsporidia, encephalitozoon cuniculi from mammals and glugea atherinae and spraguea lophii from fishes, were investigated. high phospholipid levels were found (54.8-64.5% of total lipids), which is in agreement with the presence of highly developed internal membranes in microsporidian spores. sphingomyelin was not detected in g. atherinae. triglycerides (less than 10% of total lipids), cholesterol, and free fatty acids were identified in all species. analysis of fatty acids ... | 2001 | 11426718 |
| development, characterization and future prospects of monoclonal antibodies against spores of glugea atherinae (protozoa-microsporidia-fish parasites). | monoclonal antibodies against spores of glugea atherinae were obtained after lymphocytic hybridization made from immunized mouse splenocytes. screening using an indirect enzyme linked immunosorbent assay (elisa), revealed seven monoclonal antibodies with an intense but variable reaction with the spores of fish microsporidia, and a moderate reaction with those of an insect microsporidium (nosema sp.). the reaction was weaker with spores of encephalitozoon intestinalis found in hiv+ patients. fitc ... | 1997 | 9435136 |
| identification and phylogenetic relationships of microsporidia by riboprinting. | the ssurdna and the its of different microsporidia from eight fishes, four insects and a shrimp were amplified and digested with restriction enzymes. the generated riboprints suggest a close evolutionary relationship between glugea americanus and spraguea lophii suggesting that glugea americanus should be renamed spraguea americanus and that the tissue infected and host origin should be considered of greater taxonomic importance for defining a genus than previously considered. phylogenetic analy ... | 1997 | 9435126 |
| comparative study of microsporidian spores by flow cytometric analysis. | spore suspensions of microsporidian parasites of fish (microsporidium ovoideum, glugea stephani, glugea atherinae and spraguea lophii) have been analyzed by flow cytometry. spore nuclei were dyed either by propidium iodide or bis-benzimide (hoechst 33342). by observation of forward light scatter and fluorescence the four species could be distinguished and the mono- and diplokaryotic populations of s. lophii identified. staining of dna by bis-benzimide was better and easier than propidium iodide. ... | 1994 | 8049684 |
| use of cross-reactive antigens of the microsporidian glugea atherinae for the possible detection of enterocytozoon bieneusi by western blot. | the microsporidia enterocytozoon bieneusi is reported in 10-30% of those infected with the human immunodeficiency virus. the parasite appears to be a cause of gastralgia, malabsorption, and diarrhea. a western blot technique using another microsporidian species, glugea atherinae, has demonstrated an antigenic similarity between this parasite and e. bieneusi. preliminary results show the variability of the antigenic profiles obtained from the sera of immunodeficient patients infected with e. bien ... | 1995 | 7856832 |
| fatty acid composition of four microsporidian species compared to that of their host fishes. | the fatty acid composition of four microsporidian species (glugea atherinae, spraguea lophii, glugea americanus, and pleistophora mirandellae) and their host fishes has been determined using gas chromatography. twenty-four fatty acids were identified with differences in relative abundance of fatty acids among the four parasites. certain even-saturated fatty acids were found in a very high proportion: palmitic acid (16:0) represented one-third of total fatty acids in pleistophora mirandellae. the ... | 2000 | 10651288 |
| on small genomes in eukaryotic organisms: molecular karyotypes of two microsporidian species (protozoa) parasites of vertebrates. | pulsed field gel electrophoresis (pfge) was used to separate chromosome-sized dna from two species of microsporidia of fishes. the molecular karyotype of glugea atherinae exhibits 16 dna bands from 420 to 2,700 kb, and that of spraguea lophii 12 bands from 230 to 980 kb. until now they represent respectively the largest and the smallest genomes visualized for microsporidia: 19.5 mb for g. atherinae and 6.2 mb for s. lophii (the smallest nuclear genome in eukaryotic organism). we have analysed se ... | 1994 | 7994619 |