| non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes. | alkaline phosphatases from different trematodes occupying the same habitat have identical ph otima but different levels of enzyme activities. isoparorchis hypselobagri, from the fish wallago attu, shows four to six times more enzyme activity than fasciolopsis buski, gastrodiscoides hominis and echinostoma malayanum, from the pig sus scrofa, and fasciola gigantica, gigantocotyle explanatum, cotylophoron cotylophorum and gastrothylax crumenifer, from the buffalo bubalus bubalis. at least two peaks ... | 1975 | 1442 |
| studies on in vitro survival of isoparorchis hypselobagri. | adult parasites, isoparorchis hypselobagri were maintained in vitro in four basic salt solutions, viz. stephenson saline, ab saline, tyrode's solution and locke's solution. survival was prolonged by adding sugars to tyrode's solution. of all the sugars, glucose was found most effective and parasites survived for 39 days in solution containing glucose, 6 days in galactose and sucrose, 10 days in fructose and lactose and 12 days in maltose. the parasites absorb carbohydrates through the cuticle. l ... | 1977 | 18849 |
| polyphenol oxidase and scleroprotein/melanin pigments of isoparorchis hypselobagri. | the polyphenol oxidase activity of isoparorchis hypselobagri was estimated biochemically and was believed to be associated with egg-shell formation in the parasite. the eggs gave positive tests to color reaction of scleroprotein/melanin. | 1978 | 207031 |
| quantitative studies on acetylcholinesterase in seven species of digenetic trematodes. | quantitative estimation of absolute levels and in vitro release of acetylcholinesterase (ache) in seven species of digenetic trematodes: isoparorchis hypselobagri from the swim bladder of catfish, wallago attu; srivastavaia indica and gastrothylax crumenifer from the rumen, and gigantocotyle explanatum from the liver of the water buffalo, bubalus bubalis; fasciolopsis buski, echinostoma malayanum from the small intestine and gastrodiscoides hominis from the caecum of the pig, sus scrofa reveale ... | 1977 | 562036 |
| alkali denaturation of oxyhaemoglobins of some digenetic trematodes and their hosts. | oxyhaemoglobins of six digenetic trematodes,--srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri, and of their 3 vertebrate hosts, bubalus bubalis, sus scrofa, wallago attu, were subjected to alkali denaturation at a ph of 12.4. all oxyhaemoglobins from trematodes and their hosts differ from each other in the rate and extent of alkali denaturation which may be explained due to variations in the amino acid ... | 1977 | 599278 |
| egg-shell formation in isoparorchis hypselobagri. | egg-shell formation in isoparorchis hypselobagri is descirbed. the egg-shell in isoparorchis hypselobagri is colorless and transparent as in case of diplodiscus mehrai (madhavi, 1968). eleven histochemical tests were used to elucidate the process and it was observed that the basic mechanism of egg-shell formation followed the quinone tanned system as all the three precursors i.e. proteins, phenols and phenolase were present in vitelline globules. | 1976 | 817526 |
| effect of anoxic incubation on the oxygen uptake of isoparorchis hypselobagri (digenea: trematoda). | anoxic incubation for varying periods of isoparorchis hypselobagri from the swim bladder of the catfish wallago attu, led to an accumulation of oxygen debt on the part of the parasite. with increasing length of anoxic incubation, there is a corresponding increase in post-anaerobic oxygen uptake, and the longer the period of incubation is, the greater is the time taken to return to a normal rate of oxygen uptake. | 1977 | 919680 |
| qualitative analysis of substances excreted by isoparorchis hypselobagri (trematoda) during aerobic in vitro culture. | isoparorchis hypselobagri from the swim bladder of the catfish, wallago attu was aerobically cultured in vitro. the dialyzed incubate was microchemically analysed for either individual or group of compounds excreted by the parasite. amino acids, amines, amides, ammonia, aldehyde, glycine, alpha-alanine, tyrosine, alpha-aminocarboxylic acids, haemoglobin, nitrogen, reducing materials, and lactic, pyruvic, succinic, propionic and acetic acids were found in the incubate, but acetaldehyde, formaldeh ... | 1976 | 961002 |
| spectrophotometric analysis of haemoglobins of some digenetic trematodes and their hosts. | the haemoglobins of six different species of trematodes: gastrothylax crumenifer, srivastavaia indica, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri and their three different hosts: bubalus bubalis, sus scrofa, and wallago attu were spectrophotometrically investigated, and were found to contain porphyrin ix as the common prosthetic group. oxyhaemoglobin, carbonmonoxy-haemoglobin and reduced haemoglobin of all 6 species of trematodes and their 3 ... | 1976 | 1010924 |
| gas content of swim bladder of wallago attu and oxygen consumption in isoparorchis hypselobagri (trematoda). | the swim bladder gas of wallago attu contains o2 (22-58 mm hg) and the presence or absence of isoparorchis hypselobagri does not influence the o2 content of the swim bladder. glucose increases the o2 consumption of i. hypselobagri by 50%. with the passage of in vitro culture time, the rate of o2 consumption gradually decreases to the extent of 64% on the 40th day. the optimal temperature for o2 consumption is 30 degrees c beyond which the rise in temperature is detrimental to the fish trematode ... | 1975 | 1210529 |
| trace elements in isoparorchis hypselobagri. | the trace elements contents of the tissue of isoparorchis hypselobagri were ascertained by quantitative analyses. the iron content was found to be particularly high in comparison of copper and zinc. | 1976 | 1258527 |
| protein metabolism in isoparorchis hypselobagri. | isoparorchis hypselobagri was maintained in a non-nutrient medium containing streptomycin and penicillin to prevent bacterial growth for a period of 12 hours. the parasites were kept under aerobic conditions at a suitable temperature of 30 degrees c. the protein content in isoparorchis hypselobagri ranged from 56.25% to 67.5% with an average of 63.5%+/-4.6 of dry seight of the tissue. the nitrogen of isoparorchis hypselobagri excreted as ammonia, after 12 hours of starvation was 3.04% of the tot ... | 1976 | 1274386 |
| amino acid composition in isoparorchis hypselobagri. | the free amino acids of isoparorchis hypselobagri have been determined by the chromatographic method (two-dimensional ascending). these are: leucine, valine, proline, alanine, glycine, glutamic acid and aspartic acid. | 1976 | 1274387 |
| isoparorchis hypselobagri: ultrastructure of the tegument and associated tissues in a digenean inhabiting a gaseous aerobic environment. | adult i. hypselobagri live in the swim bladder of the indian catfish wallago attu, a gaseous environment with a relatively high oxygen content. the ventral tegument, which in life is applied close to the swim bladder wall, is relatively unspecialized, showing typical ultrastructural features of the digenean surface. the dorsal tegument, which is exposed to the oxygen-rich surroundings, has numerous pyriform extensions of superficial parenchymal cells closely applied to the base of the surface sy ... | 1990 | 2242957 |
| some trematodes of freshwater fishes from north vietnam with a list of recorded endohelminths by fish hosts. | the present paper comprises a systematic survey of trematodes collected from 12 species of freshwater fishes from north vietnam (the red river near hanoi). altogether 16 trematode species (14 adults and 2 juvenile forms) have been recorded. these include: amurotrema dombrowskajae, platycladorchis microacetabularis, p. macroacetabularis, neocladorchis multilobularis, carassotrema koreanum, azygia hwangtsiyui, phyllodistomum clariasi, phyllodistomum sp., orientocreadium batrachoides, masenia colla ... | 1989 | 2583614 |
| ascorbic acid levels of trematode parasites of fish and mammalian hosts. | ascorbic acid levels of seven species of digenetic trematodes, living under different environmental conditions in fish and mammals were determined colorimetrically. ascorbic acid levels ranged from 0.312 mg/100 g fresh weight in gastrothylax crumenifer to 5.070 mg/100 g fresh weight in fasciola indica. low levels of ascorbic acid were detected in the rumen parasites and in isoparorchis hypselobagri. in contrast the liver parasites were found to have relatively high ascorbic acid levels. | 1980 | 7395312 |
| effects of some sugars, amino acids, glycerol and alpha-glycerophosphate on oxygen uptake of some digenetic trematodes. | effect of various substrates on the oxygen uptake of some digenetic trematodes -- srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum from the buffalo, bubalus bubalis and isoparorchis hypselobagri from the fish wallago attu was studied. in all the four species, the effect of various substrates except maltose was found to be stimulatory. the inhibitory or stimulatory effect of these substrates on oxygen uptake reflects the importance of these compounds in the energy metabolism ... | 1980 | 7450612 |
| structural and functional comparison of trematode haemoglobins. | haemoglobins from three different species of trematodes (gastrothylax crumenifer, paramphistomum epiclitum and isoparorchis hypselobagri) and their respective hosts were digested with papain. peptides so produced were separated on 10-15% sds-pagge. the probability of coincidence of the peptides was analysed statistically. oxygen affinity curves of trematode haemoglobins were found to be a rectangular hyperbolic. the hill coefficient values of all the trematode haemoglobins were found to be 1. th ... | 1995 | 7622793 |
| purification and properties of the hemoglobins of the platyhelminth isoparorchis hypselobagri (trematoda: isoparorchidae) and its host wallagu attu (catfish). | 1. the hemoglobins of the trematode isoparorchis hypselobagri and of its host wallagu attu (catfish) were isolated and purified. 2. sds-polyacrylamide gel electrophoresis showed both to consist of single, 15-17 kda chains, having different electrophoretic mobilities. 3. isoelectric focusing showed the trematode hemoglobin to be homogeneous with a pi of 4.2 and the host hemoglobin to consist of several components. 4. gel filtration of freshly prepared trematode hemoglobin revealed one peak corres ... | 1993 | 8299358 |
| trematode myoglobins, functional molecules with a distal tyrosine. | the myoglobins of two trematodes, paramphistomum epiclitum and isoparorchis hypselobagri, were isolated to homogeneity. the native molecules are monomeric with mr 16,000-17,000 and pi 6.5-7.5. in each species, at least four different globin isoforms occur. primary structure was determined at the protein level. the globin chains contain 147 amino acid residues. although major determinants of the globin fold are conserved, characteristic substitutions are present. a tyr residue occurs at the helic ... | 1997 | 9006947 |
| trematode hemoglobins show exceptionally high oxygen affinity. | ligand binding studies were made with hemoglobin (hb) isolated from trematode species gastrothylax crumenifer (gc), paramphistomum epiclitum (pe), explanatum explanatum (ee), parasitic worms of water buffalo bubalus bubalis, and isoparorchis hypselobagri (ih) parasitic in the catfish wallago attu. the kinetics of oxygen and carbon monoxide binding show very fast association rates. whereas oxygen can be displaced on a millisecond time scale from human hb at 25 degrees c, the dissociation of oxyge ... | 1998 | 9675199 |
| molecular cloning and immunolocalization of the 17 kda myoglobin of clonorchis sinensis. | we purified the 17 kda protein abundant in clonorchis sinensis crude extracts. the n-terminal amino acid sequence of this protein was determined and an oligonucleotide probe synthesized. using this probe, the cdna encoding the protein was cloned and sequenced from the c. sinensis cdna library. it was found to consist of a total of 150 amino acids and to have 41% conserved homology with the myoglobin of the trematodes paramphistomum epiclitum and isoparorchis hypselobagri. the gene product over-e ... | 2003 | 12720088 |