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effect of carbon and nitrogen sources on the production of penitrem b by penicillium aurantiogriseum.effect of different carbon and nitrogen sources on the production of penitrem b was studied. d-xylose induced maximum penitrem b production, while melibiose, glycerol, citric acid and succinic acid were poor substrates. potassium nitrate, l-asparagine, sodium nitrate, glycine, dl-aspartic acid and l-tryptophan supported good production of penitrem b. conversely zirconyl nitrate, barium nitrate, aluminum nitrate, acetanilide, 4-aminobenzoic acid, 4-nitrobenzoic acid and 4-nitroaniline were toxic ...19921505862
imi descriptions of fungi and bacteria no. 1101. penicillium aurantiogriseum. 19921640978
persistent karyomegaly caused by penicillium nephrotoxins in the rat.continuous or intermittent consumption by rats of food moulded by penicillium aurantiogriseum induced prominent and extensive histopathological changes within several weeks seen specifically at the renal cortico-medullary junction. many cells of the p3 segment of proximal tubules contained either giant nuclei or multiple enlarged nuclei, described in this text as karyomegaly, but also included within a cytomegalic change. the changes contrasted with the tubular cell necrosis and concomitant mito ...19911686091
comparative acute nephrotoxicity of penicillium aurantiogriseum in rats and hamsters.air-dried mycelium of penicillium aurantiogriseum2, grown as a surface culture on yeast extract-sucrose medium, was incorporated into powdered diet and fed to rats and hamsters for different periods up to 28 days. at intervals, animals were anaesthetized and the kidneys fixed in situ by perfusion. in rats, the fungus produced scattered exfoliation of pyknotic cells and an increased frequency of mitotic figures involving the pars recta segment of proximal tubular epithelium. this lesion was detec ...19911820323
penicillium aurantiogriseum-induced, persistent renal histopathological changes in rats; an experimental model for balkan endemic nephropathy competitive with ochratoxin a.renal histopathological changes in rats, caused by food partially moulded by a common fungus isolated from an area of nephropathy in yugoslavia, were differentiated into acute and chronic responses. the acute response to a few days on the diet specifically involved necrosis and concomitant mitosis in proximal tubule cells. more protracted, continuous or intermittent administration of nephrotoxic mould led to a marked karyomegaly in the same corticomedullary region. the phenomenon is more promine ...19911820324
acute histopathological changes produced by penicillium aurantiogriseum nephrotoxin in the rat.shredded wheat moulded by an isolate of penicillium aurantiogriseum elicited progressive histopathological changes at the rat renal cortico-medullary junction during 5 days of dosing, when incorporated into diet as a 20% component. the changes of acute tubular necrosis and regeneration were seen in the p3 segment of the nephron. in rats exposed to contaminated diet for 5 days the histopathological changes regressed in severity by about one-half within a further 4 days on normal diet and by 7 day ...19911888665
nephrotoxicity of penicillium aurantiogriseum and p. commune from an endemic nephropathy area of yugoslavia.seven out of nine penicillium isolates from mouldy maize in yugoslavia have been differentiated into the adjacent species p. aurantiogriseum and p. commune. nephrotoxicity of cultured mycelia in the rat has been demonstrated for all isolates of both species and was correlated usefully, though indirectly, with the production of benzodiazepine secondary metabolites, notably auranthine. shredded wheat (22 g) moulded by an example of each species and fed to a rat over 4 days elicited renal pathology ...19902089255
[effect of temperature on the growth of penicillium from moldy feed].178 penicillium strains were isolated from moulded feedstuffs (mainly corn and wheat after ambient air drying, wheat after refrigeration, corn after treatment with propionic acid), and investigated for the effect of temperature on growth rate. temperatures between 4 and 27 degrees c were used. 159 strains proved psychrotrophic, they were able to grow at 4 degrees c on malt extract agar (ma) as well as in wheat; the growth in wheat was indicated by the production of ergosterol. on ma all psychrot ...19902091576
volatile metabolites and other indicators of penicillium aurantiogriseum growth on different substrates.penicillium aurantiogriseum dierckx was cultivated on six agar substrates (barley meal agar, oat meal agar, wheat meal agar, malt extract agar, czapek agar, and norkrans agar) and on oat grain for 5 days in cultivation vessels provided with an inlet and an outlet for air. volatile metabolites produced by the cultures were collected on a porous polymer adsorbent by passing an airstream through the vessel. volatile metabolites were collected between days 2 and 5 after inoculation. co2 production w ...19902128008
mycotoxins and toxigenic fungi on cereal grains in western canada.toxins occasionally present on cereal grains in the field in western canada include ergot alkaloids produced by claviceps purpurea and trichothecenes produced by fusarium species, particularly fusarium sporotrichiodes and fusarium graminearum. ht-2 toxin, t-2 toxin, diacetoxyscirpenol, and deoxynivalenol are the main trichothecenes encountered. during storage of cereals, the predominant toxins and toxigenic fungi are ochratoxin a and citrinin produced by penicillium aurantiogriseum, p. chrysogen ...19902200591
[detection of cyclopiazonic acid and its producers in food].in the course of six months, 60 samples of foods were examined for their contents of cyclopiazonic acid. these samples were subjected to a basal mycological screening aimed at aspergillus flavus and penicillium sp. strains. cyclopiazonic acid contents in samples of hermelín cheese, peanuts, rice, peeled barley grains, folican salami, and packaged meat did not exceed the value of 0.5 mg.kg-1. when using a modification of the method of cyclopiazonic acid isolation described by dorner et al. (1983) ...19892508296
volatile metabolites of some barley storage molds.volatile metabolites from penicillium species grown on barley were concentrated by adsorption on tenax and analyzed by gas chromatography and gas chromatography/mass spectrometry. of the compounds identified from penicillium aurantiogriseum, p. verrucosum and p. viridicatum, 3-methyl-1-butanol (mb), styrene (s) and 1-octen-3-ol (oo) were prominent while smaller amounts of 2-pentylfuran (pf), 3-methylanisole (ma), 2-(2-furyl)pentanal (fp) and 2-ethyl-5-methylphenol (emp) were detected. p. coproph ...19892641484
nephrotoxicity of penicillium aurantiogriseum, a possible factor in the aetiology of balkan endemic nephropathy.water-soluble components of a nephrotoxic isolate of penicillium aurantiogriseum have been fractionated by sequential ion-exchange, size-exclusion gel filtration, reverse-phase silica chromatography and hplc. nephrotoxicity in the rat was confined to a size-exclusion fraction approximating to 1,500 daltons, which also inhibited dna synthesis in cultured kidney cells. the more sensitive in vitro assay allowed toxicity to be followed to a sub-fraction from gradient-elution hplc which in further hp ...19883419480
renal histopathological responses to nephrotoxic penicillium aurantiogriseum in the rat during pregnancy, lactation and after weaning.the typical renal histopathological changes in proximal tubules of rats consuming food containing shredded wheat moulded by penicillium aurantiogriseum did not occur in neonates of rats ingesting the nephrotoxic diet during pregnancy nor in pups fed only by lactation from a treated dam. in contrast, weanlings consuming the moulded diet consistently showed within a few days densely staining mitosis-like structures in the proximal tubules which was the first step leading to development of the prom ...19948107961
effects of potassium sorbate on growth and penicillic acid production by aspergillus ochraceus and penicillium aurantiogriseum.the effect of potassium sorbate on the growth and penicillin acid production of aspergillus ochraceus and penicillium aurantiogriseum was studied. yeast extract sucrose (yes) broth at initial ph values of 5.5 or 7.0, and containing different concentrations of potassium sorbate was inoculated with fungal spores and incubated for 35 days at 28 degrees c. in all cases, although the ph changes in sorbate-containing media were delayed, patterns were similar to those of the cultures without sorbate. t ...19938212939
lipolytic activity and degradation of rapeseed oil and rapeseed by spoilage fungi.aspergillus, eurotium and penicillium spp. from rapeseed were able to grow and produce lipases over a range of water activities (aw) at both 15 and 25 degrees c on tributyrin agar. the ability to produce lipases was not directly related to growth rate. the clearing zone: growth rate ratios gave lipase indices which varied markedly between test fungi and with aw. the fungi with the highest indices were aspergillus candidus and aspergillus versicolor and penicillium expansum and penicillium hordei ...19938217518
composition and toxigenic potential of the mould population on dry-cured iberian ham.the fungal population on dry-cured iberian ham can be essential to the development of the product's unique characteristics, but health hazards due to mycotoxins may be significant. we examined the natural fungal population of iberian hams during ripening at three different locations. chloroform extracts from 59 selected isolates were tested for toxicity to brine shrimp larvae and vero cells, for mutagenicity in the ames test and for antimicrobial activity against staphylococcus aureus. the diver ...19968880338
interactive effects of propionic acid and temperature on growth and ochratoxin a production by penicillium aurantiogriseum.propionate at concentrations up to 0.05% decreased at 25 degrees c the growth and sporulation of penicillium aurantiogriseum. the standard size of conidiogenous structures (metulae, phialids) and conidia was diminished. the effect was more pronounced at a higher temperature (30 degrees c). inhibition of ochratoxin production by propionate was also demonstrated.19958919930
two-step hydrolysis of amygdalin in molds.mucor circinelloides lu m40 and penicillium aurantiogriseum p 35, characterized by extracellular beta-glucosidase activity on cyanogenic glycosides, hydrolyse amygdalin by a two-step reaction mechanism being the first step of hydrolysis, from amygdalin to prunasin, very rapid (15 min) and the second one, from prunasin to mandelonitrile, much slower (120 min).19969122587
amygdalin degradation by mucor circinelloides and penicillium aurantiogriseum: mechanisms of hydrolysis.mucor circinelloides lu m40 and penicillium aurantiogriseum p 35 produce extracellular beta-glycosidases that are active on the cyanogenic glycoside amygdalin. from the culture broths of m. circinelloides, only one beta-glycosidase could be identified, while two different enzymes - both having amygdalase activity - were found in culture broths of p. aurantiogriseum. the study of the mechanism of hydrolysis of the beta-bis-glycoside amygdalin with purified enzymes from the two organisms indicated ...19989446681
production and properties of the linamarase and amygdalase activities of penicillium aurantiogriseum p35.the effects of medium composition on the production of beta-glucosidase (amygdalase and linamarase) by penicillium aurantiogriseum p35 were studied and the medium optimized as follows (g/l of deionized water): pectin, 10.0; (nh4)2so4, 8.0; kh2po4, 8.0; na2hpo4, 2.8; mgso4.7h2o, 0.5; yeast extract, 4.0; initial ph 6.0. when grown in a bench fermenter on this medium, the fungus produced 50.5 mu of amygdalase and 9.4 mu of linamarase per ml of culture broth. two beta-glucosidases (pgi and pgii), ea ...199910380623
uv-guided isolation of verrucines a and b, novel quinazolines from penicillium verrucosum structurally related to anacine from penicilliumaurantiogriseumtwo novel quinazolines, verrucines a (1) and b (2), have been isolated as a major and a minor metabolite, respectively, of penicillium verrucosum. both are condensates of one mole each of anthranilic acid, phenylalanine, and glutamine. the structures were elucidated by spectroscopic methods, and the two compounds were found to be epimers. the spectroscopic data for the ostensible benzodiazepine anacine reported from penicillium aurantiogriseum, composed of one mole each of anthranilic acid, leuc ...199910579880
impact of environmental factors on fungal respiration and dry matter losses in wheat straw.an automatic electrolytic respirometer enabled replicated determinations of the respiration rates of individual fungi on sterile straw, and the mixed mycoflora of naturally contaminated wheat straw at different steady-state temperatures (10-30 degrees c) and water activities (a(w), 0.75-0.98) over periods of 8-14 days. generally, the respiratory activity of individual spoilage fungi (alternaria alternata, cladosporium cladosporioides, eurotium amstelodami, fusarium culmorum and penicillium auran ...200011124368
effect of water activity and temperature on growth of three penicillium species and aspergillus flavus on a sponge cake analogue.this study compared the effect of temperature and water activity and their interactions on the rate of mycelial growth of penicillium aurantiogriseum, p. chrysogenum, p. corylophilum and aspergillus flavus on a sponge cake analogue. as expected, growth rates showed dependence on a(w), and temperature. however, no significant differences were observed in the growth rates of different isolates. the minimum a(w) values for growth of the penicillium spp. was 0.85-0.90. a. flavus was able to grow at ...200111789933
anicequol, a novel inhibitor for anchorage-independent growth of tumor cells from penicillium aurantiogriseum dierckx tp-f0213.a novel inhibitor for anchorage-independent growth of tumor cells was isolated from the culture broth of a fungal strain. the producing strain tp-f0213 was identified as penicillium aurantiogriseum dierckx based on the taxonomic study. the compound designated anicequol was obtained by solvent extraction, hp-20 and silica gel chromatographies and recrystallization. the planar structure was elucidated by nmr analysis to be 16-acetoxy-3,7,11-trihydroxyergost-22-en-6-one. the absolute configuration ...200212061544
[isolation of penicillium dierckx 1901--a producer of diketopiperazine alkaloids rokefortin and 3,12-dihydrokefortin--from permafrost].secondary metabolites of the three strains of penicillium aurantiogriseum, isolated from permafrost sediments, were identified. it was found that these fungi synthesized diketopiperazine alkaloids roquefortine and 3,12-dihydroroquefortine. the strain vkm fw-766 synthesized alkaloids in in the course of certain growth-related processes. when the strain was grown on a mineral medium, time courses of roquefortine and 3,12-dihydroroquefortine concentrations were characterized by biphasic curves.200314520965
evaluation of some fungi and bacteria for biocontrol of anthracnose disease of cowpea.the efficacy of some fungal and bacterial isolates obtained from cowpea phylloplane in inhibiting the in vitro and in vivo growth of colletotrichum lindemuthianum, causal agent of anthracnose of cowpea was investigated. inhibition of growth of the pathogen with production of zones of inhibition was observed for aspergillus flavus, a. ochraceus, penicillium aurantiogriseum, bacillus subtilis-bs21, b. subtilis-bs22 and b. subtilis-bs23. inhibition of growth on contact was recorded for a. niger whi ...200414768021
ecological determinants for germination and growth of some aspergillus and penicillium spp. from maize grain.this study compared the effect of temperature (5-45 degrees c), water availability (water activity, aw; 0.995-0.75) and their interactions on the temporal rates of germination and mycelial growth of three mycotoxigenic strains of aspergillus ochraceus and one isolate each of a. flavus, a. niger, penicillium aurantiogriseum and p. hordei in vitro on a maize extract medium. germination was very rapid at > 0.90 aw with an almost linear increase with time for all species. however, at < 0.90 aw, the ...199815244054
[the alkaloids of penicillium aurantiogriseum dierckx (1901) var. aurantiogriseum vkm f-1298].the fungus penicillium aurantiogriseum var. aurantiogriseum vkm f-1298 produces two benzodiazepine alkaloids (anacine and aurantine) and one diketopiperazine alkaloid (aurantiamine). when cultured in a submerged mode in abe medium, the alkaloids are mostly secreted into the medium. the dynamics of aurantine and aurantiamine accumulation in the medium is characterized by the presence of a relatively sharp maximum in the idiophase, whereas the accumulation of anacine in the medium is characterized ...200415521175
mites and fungi in heavily infested stores in the czech republic.toxigenic and allergen-producing fungi represent a serious hazard to human food and animal feed safety. ninety-four fungal species were isolated from mite-infested samples of seeds taken from czech seed stores. fungi were isolated from the surface of four kinds of seeds (wheat, poppy, lettuce, and mustard) and from the gut and external surface of five species of mites (i.e., acarus siro l., 1758, caloglyphus rhizoglyphoides (zachvatkin, 1973), lepidoglyphus destructor (schrank, 1781), tyrophagus ...200415666776
fungi recovered from slovakian poultry feed mixtures and their toxinogenity.to contribute towards the knowledge of microbiology of feeds, more than 100 samples of poultry feed mixtures from slovakia were mycologically investigated in terms of the overall fungal diversity and toxicological potential of isolated fungi. the study revealed that out of 22 genera recovered, penicillium was the most frequent and diverse genus, followed by aspergillus and mucor being found in 89 % (34 spp.), 69 % (11 spp.) and 50 % (4 spp.), respectively. the most frequently encountered taxa we ...200617195991
aurantiomides a-c, quinazoline alkaloids from the sponge-derived fungus penicillium aurantiogriseum sp0-19.three new quinazoline alkaloids, aurantiomides a (1), b (2), and c (3), along with the known metabolite anacine (4) were isolated from the sponge-derived fungus strain penicillium aurantiogriseum sp0-19 by bioassay-guided fractionation. their structures were elucidated by spectroscopic and chemical methods. their absolute configurations were deduced by comparison of their cotton effects with anacine (4) and by chemical transformations. compounds 2 and 3 showed moderate cytotoxicities against hl- ...200717455978
multispecies interactions affect cytoplasmic incompatibility in tribolium flour beetles.previous studies established that cytoplasmic incompatibility in the confused flour beetle, tribolium confusum, is mediated by a maternally inherited rickettsia-like bacteria: crosses between infected males and uninfected females are sterile. all other crosses are fertile, and individuals can be cured of infection by treatment with tetracycline. we report that a third species-either actinomycete bacteria streptomyces or fungi penicillium-can cure beetles of infection with these parasites. progen ...199219426036
mycotoxic nephropathy in bulgarian pigs and chickens: complex aetiology and similarity to balkan endemic nephropathy.spontaneous nephropathy in bulgaria, which is observed frequently during meat inspection and which differs morphologically from the classical description of mycotoxic porcine/chicken nephropathy as made in denmark, was found to have a multi-mycotoxic aetiology being mainly provoked by a combined effect of ochratoxin a, penicillic acid and fumonisin b1 in addition to a not-yet-known metabolite. mean contamination levels of ochratoxin a were consecutively low (188.8 and 376.4 microg kg(-1)) in con ...201019753495
assessment of alcohol percentage test for fungal surface hydrophobicity measurement.to determine whether assessing the penetration of solutions with different concentrations of ethanol (alcohol percentage test: apt) on fungal surfaces is effective in characterization of hydrophobicity on fungal surfaces.201020070510
[effects of pa-pex 11 gene on penicillin production in penicillium aurantiogriseum].to study the effect of pa-pex 11 gene on penicillin production in pencillium aurantiogriseum.200920112676
first morphomolecular identification of penicillium griseofulvum and penicillium aurantiogriseum toxicogenic isolates associated with blue mold on apple.postharvest blue mold decay caused by penicillium spp. is the most important disease of fresh apple fruit in the world, which extend from the field to the store. two new penicillium spp. responsible for apple fruit decay were recovered. the morphological and molecular features of penicillium griseofulvum and penicillium aurantiogriseum isolated from apple fruits were characterized morphologically and molecularly. pathogenicity test exhibited that both p. griseofulvum and p. aurantiogriseum were ...201020367389
verrucisidinol and verrucosidinol acetate, two pyrone-type polyketides isolated from a marine derived fungus, penicillium aurantiogriseum.the new secondary metabolites verrucosidinol (1) and its derivative verrucosidinol acetate (2), together with a potent neurotoxin verrucosidin (3), a congener norverrucosidin (4) and a mixture of two known phytotoxic metabolites terrestric acids (5 and 6), were isolated from the marine derived fungus penicillium aurantiogriseum. verrucosidinol has a ring-opened ethylene oxide moiety in the polyene α-pyrone skeleton, and verrucosidinol acetate is its acetate derivative. the chemical structures we ...201021139842
comparative effects of native filamentous and arbuscular mycorrhizal fungi in the establishment of an autochthonous, leguminous shrub growing in a metal-contaminated soil.the aim of this study was to assess the effectiveness of inoculation with a native arbuscular mycorrhizal (am) fungus, glomus mosseae (nicol. and gerd.) gerd. and trappe, or a filamentous fungus, penicillium aurantiogriseum dierckx 1901, on the establishment of coronilla juncea l. seedlings grown in a polluted, semiarid soil. for that, root and shoot biomass, nutrient uptake, mycorrhizal colonisation and nitrate reductase (nr) and phosphatase activities were analysed. six months after planting, ...201121211827
synthesis of sugars embodying conjugated carbonyl systems and related triazole derivatives from carboxymethyl glycoside lactones. evaluation of their antimicrobial activity and toxicity.the synthesis of a series of pyranoid derivatives comprising a conjugated carbonyl function and related triazole derivatives, structurally suitable for bioactivity evaluation, was achieved in few steps starting from readily available carboxymethyl glycoside lactones (cmgl). 3-enopyranosid-2-uloses were generated by oxidation/elimination of tri-o-acylated 2-hydroxy pyranosides. subsequent wittig olefination provided stereoselectively 2-c-branched-chain conjugated dienepyranosides with (e)-configu ...201021215645
rhizosphere dynamics during phytoremediation of olive mill wastewater.the potential of phytoremediation as a treatment option for olive mill wastewater (omw) was tested on five perennial tree species. cupressus sempervirens and quercus ilex proved tolerant to six-month omw treatment followed by six-month water irrigation, whereas salix sp. and laurus nobilis and, later, pinus mugo suffered from phytotoxic effects. test plants were compared to controls after treatment and irrigation, by monitoring biochemical and microbiological variations in the rhizosphere soil. ...201021256739
fermentation medium for collagenase production by penicillium aurantiogriseum urm4622.medium composition and culture conditions for maximal collagenase production by penicillium aurantiogriseum urm4622 were optimized using a response surface approach. a full two-level design on three factors (initial medium ph, soybean flour concentration, and temperature) was employed to identify the most significant fermentation parameters for collagenase production, and a subsequent central composite design (ccd) was used to find the optimal levels of the two most significant factors (initial ...201121774096
microbial and physico-chemical changes during the ripening of dry fermented sausages superficially inoculated with or having added an intracellular cell-free extract of penicillium aurantiogriseum.the effect of inoculation of the "salchichón" (dry fermented sausage) surface with an atoxygenic, proteolytic and lipolytic strain of penicillium aurantiogriseum and/or the addition of an intracellular cell-free-extract (icfe) of the same mould on the ripening process was studied. four batches of salchichón were manufactured: control, superficially inoculated, added with the intracellular cell free extract and combination of both treatments. superficial mould modified micrococcaceae counts, ph, ...200122062509
combined use of pronase e and a fungal extract (penicillium aurantiogriseum) to potentiate the sensory characteristics of dry fermented sausages.a combination of an extract of penicillium aurantiogriseum and pronase e was used in an attempt to enhance the sensory characteristics of dry fermented sausages, specifically, a salami-like one named "salchichón". the addition of pronase e alone (600 units/kg) produced a rise in free amino acids and biogenic amines and also an increase in the ammonia content. addition of the protease and fungal extract (100.87 mg protein/kg of mixture) brought about a decrease in the level of free amino acids an ...200022060609
the contribution of penicillium aurantiogriseum to the volatile composition and sensory quality of dry fermented sausages.an atoxygenic, proteolytic and lipolytic strain of penicillium aurantiogriseum was tested for its ability to accelerate the production of volatile compounds and to improve the sensory properties of dry fermented sausages. the following batches of sausages were prepared: control; superficially inoculated with a spore suspension; added with an intracellular cell free extract; and superficially inoculated and added with the intracellular cell free extract. higher levels of lipid oxidation products ...200122062510
antimicrobial and radical scavenging properties of bovine collagen hydrolysates produced by penicillium aurantiogriseum urm 4622 collagenase.a 2(3) full factorial design was used to identify the main effects and interactions of ph, collagen concentration and temperature on the degree of collagen hydrolysis (dh) by collagenase from penicillium aurantiogriseum urm 4622. increases in both ph and collagen concentration improved dh, and a positive interaction effect was observed for these variables. on the other hand, temperature had a negative main effect on dh. the maximum value of dh (4.65 μg/ml) was achieved at 7.5 mg/ml collagen conc ...201426139912
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
control of household mycoflora in fermented sausages using phenolic fractions from olive mill wastewaters.biopreservation using polyphenols represents an alternative to chemical molecules for improving food safety. in this work, we evaluated the antifungal activity of polyphenols extracted from olive mill wastewater (omwwp) to reduce or eliminate the growth of undesired fungi on the surface of dry fermented sausages. antagonism against penicillium expansum dsmz 1282, penicillium verrucosum dsmz 12639, penicillium nalgiovense ms01, aspergillus ochraceus dsmz 63304, cladosporium cladosporioides ms12, ...201525996624
assessment of susceptibility of mould isolates towards biocides.the aim of this study was to determine the susceptibility of 77 mould strains: aspergillus fumigatus (20), aspergillus flavus (8), aspergillus niger (4), aspergillus ochraceus (2), penicillium citrinum (15), penicillium crysogenum (14), penicillium aurantiogriseum (1), penicillium roquefortii (4), penicillium paneum (2), rhizopus spp. (3), tricoderma spp. (1) and mucor spp. (3) to biocides.201526420602
fungi in housefly (musca domestica l.) as a disease risk indicator-a case study in south africa.houseflies are the commonest insects which have increasingly overcrowded human dwellings, particularly in rural areas and constitute a health hazard. in the environment they move back and forth by feeding and breeding on food commodities and filth. this may lead to the spread of diseases and also mycotoxin-producing fungi. thus frequent exposure to the activity of such houseflies will have an impact on the welfare of humans. the study investigated the natural occurrence of fungal contamination i ...201425193135
effect of fungal mycelia on the hplc-uv and uv-vis spectrophotometric assessment of mycelium-bound epoxide hydrolase using glycidyl phenyl ether.the use of mycelia as biocatalysts has technical and economic advantages. however, there are several difficulties in obtaining accurate results in mycelium-catalysed reactions. firstly, sample extraction, indispensable because of the presence of mycelia, can bring into the extract components with a similar structure to that of the analyte of interest; secondly, mycelia can influence the recovery of the analyte. we prepared calibration standards of 3-phenoxy-1,2-propanediol (ppd) in the pure solv ...201626902669
chemical composition and biological activity of the essential oil from leaves of moringa oleifera lam. cultivated in mozambique.the antioxidant capacity and antimicrobial activity of the essential oil of moringa oleifera (moringaceae) grown in mozambique was investigated. the chemical composition was studied by means of gc and gc-ms analysis. hexacosane (13.9%), pentacosane (13.3%) and heptacosane (11.4%) were the main components. ultra high performance chromatography-dad analysis detected the flavonoids quercetin (126 μg/g) and luteolin (6.2 μg/g). the essential oil exhibited a relatively low free radical scavenging cap ...201324022760
complex etiology and pathology of mycotoxic nephropathy in south african pigs.spontaneous nephropathy in pigs seen in south africa was found to have multi-mycotoxic etiology involving several mycotoxins such as ochratoxin a (ota), penicillic acid (pa) and fumonisin b1 (fb1) in addition to a not yet identified mycotoxin. contamination levels of ota were comparatively low (67-75 μg/kg) in contrast to high contamination levels of fb1 (5,289-5,021 μg/kg) and pa (149-251 μg/kg). a heavy contamination with gibberella fujikuroi var. moniliformis and penicillium aurantiogriseum c ...201023605239
discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen.sulfhydryl oxidases (sox) are fad-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. although the quantity of scientific papers and suggested applications for sox is constantly increasing, only a limited number of microbial sox have been reported and are commercially available. hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal sox. the screening was based on the ellman's reagent, i.e. 5,5 ...201323417429
in vitro treatments of echinococcus granulosus with fungal chitosan, as a novel biomolecule.to determined the antiparasitic activity of the isolated chitosan from penicillium viridicatum, penicillium aurantiogriseum and commercial chitosan against protoscolicidal of hydatid cysts were determined.201324075347
the anti-giardial effectiveness of fungal and commercial chitosan against giardia intestinalis cysts in vitro.chitosan with poly-n-acetylglucosamine sequences is a deacetylated derivative of chitin that can be found in the exoskeletons of crabs, shrimp and lobsters, the cuticles of insects and the cell walls of fungi. the aim of the present study was to compare the effects of fungal chitosan (fc) prepared from the cell walls of penicillium viridicatum and penicillium aurantiogriseum with commercially available chitosan (cc) against giardia intestinalis cysts in vitro. the giardia cysts were isolated usi ...201627065602
fungus-mediated synthesis of gold nanoparticles: a novel biological approach to nanoparticle synthesis.the biological effects of nanoparticles and their uses as molecular probes are research areas of growing interest. the present study demonstrates an eco-friendly biosynthesis of gold nanoparticles. the pure colonies of penicillium aurantiogriseum, penicillium citrinum, and penicillium waksmanii were cultured in fluid czapek dox broth. then, their supernatants were examined for the ability to produce gold nanoparticles. in this step, 1 mm solution of aucl added to the reaction matrixes separately ...201323646653
optimization of penicillium aurantiogriseum protease immobilization on magnetic nanoparticles for antioxidant peptides obtainment.this work reports an optimization of protease from penicillium aurantiogriseum immobilization on polyaniline coated magnetic nanoparticles for antioxidant peptides obtainment derived from bovine casein. immobilization process was optimized using a full two-level factorial design (2(4)) followed by a response surface methodology (rsm). using the derivative, casein was hydrolyzed uncovering its peptides that were sequenced and had antioxidant properties tested via abts radical scavenging and hydro ...201728278110
genome sequencing and analysis of the paclitaxel-producing endophytic fungus penicillium aurantiogriseum nrrl 62431.paclitaxel (taxol™) is an important anticancer drug with a unique mode of action. the biosynthesis of paclitaxel had been considered restricted to the taxus species until it was discovered in taxomyces andreanae, an endophytic fungus of t. brevifolia. subsequently, paclitaxel was found in hazel (corylus avellana l.) and in several other endophytic fungi. the distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis betwee ...201424460898
mycological survey of chicken feed and some feed ingredients in turkey.level of mold contamination and mycoflora were determined for 144 mixed feed and feed ingredients, including corn, sunflower cake, soja cake, meat and bone meal, and fish meal. four samples were found to be free of mold. among the feed samples examined, the mold count has been found to be low (10(2) to 10(3) colonies/g) for fish meal, high (10(4) to 10(5) colonies/g) for meat-bone meal and sunflower cake, and extremely high (more than 10(5) colonies/g) for soja cake, corn and mixed feed. the pre ...198828398859
mycoviruses of an endophytic fungus can replicate in plant cells: evolutionary implications.so far there is no record of a specific virus able to infect both fungal and plant hosts in nature. however, experimental evidence shows that some plant virus rdrps are able to perform replication in trans of genomic or di rnas in the yeast saccharomyces cerevisiae. furthermore, tobacco mosaic virus was recently shown to replicate in a filamentous ascomycetous fungus. thus, at least experimentally, some plant viruses can infect some fungi. endophytic fungi have been reported from many plants and ...201728507331
transmission of penicillium aurantiogriseum partiti-like virus 1 to a new fungal host (cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes.we attempted to transfect six recently characterized virus species to protoplasts of penicillium janczewskii and chryphonectria parasitica. none of the recovered p. janczewskii colonies was positive for the transfected viruses, but penicillium aurantiogriseum partiti-like virus 1 (paplv1) was detected in three distinct regenerated c. parasitica colonies. we screened the phenotype of the infected strains in up to 45 different conditions combining different media, salinity and temperatures: our re ...201728836717
complete nucleotide sequences of dsrna2 and dsrna7 detected in the phytopathogenic fungus sclerotium hydrophilum and their close phylogenetic relationship to a group of unclassified viruses.ten dsrna segments were extracted from sclerotium hydrophilum isolate (hz11). the isolation of virus-like particles contained 10 dsrna segments with the same number and migration as those extracted directly from the fungal mycelia. two of these dsrna segments, dsrna2 and dsrna7, were cloned and sequenced. they were 2121 and 1953 bp, respectively. the dsrna2 encodes a rna-dependent rna polymerase. the dsrna7 contains two open reading frames that encode putative proteins of unknown functions. phyl ...201627473231
collagenolytic enzymes produced by fungi: a systematic review.specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. there are few complete reports that fully uncover production, characterization and purification of fungi collagenases. in this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic or collagenase) and (fungi or fungus or fungal) and (production or synthesis or synthesize) and (ch ...201727756540
six new polyketide decalin compounds from mangrove endophytic fungus penicillium aurantiogriseum 328.six new compounds with polyketide decalin ring, peaurantiogriseols a-f (1-6), along with two known compounds, aspermytin a (7), 1-propanone,3-hydroxy-1- (1,2,4a,5,6,7,8,8a-octahydro-2,5-dihydroxy-1,2,6-trimethyl-1-naphthalenyl) (8), were isolated from the fermentation products of mangrove endophytic fungus penicillium aurantiogriseum 328#. their structures were elucidated based on their structure analysis. the absolute configurations of compounds 1 and 2 were determined by ¹h nmr analysis of the ...201526473887
quinazolin-4-one coupled with pyrrolidin-2-iminium alkaloids from marine-derived fungus penicillium aurantiogriseum.three new alkaloids, including auranomides a and b (1 and 2), a new scaffold containing quinazolin-4-one substituted with a pyrrolidin-2-iminium moiety, and auranomide c (3), as well as two known metabolites auranthine (4) and aurantiomides c (5) were isolated from the marine-derived fungus penicillium aurantiogriseum. the chemical structures of compounds 1-3 were elucidated by extensive spectroscopic methods, including ir, hresims and 2d nmr spectroscopic analysis. the absolute configurations o ...201222822373
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