| analysis of acid proteinases from aspergillus terricola. | the specific action and composition of the functional groups of active centres of three fractions of acid proteinases from aspergillus terricola have been studied. with respect to the hydrolysis rates of acetyl-l-phenylalanyl-l-tyrosine and carbobenzoxy-d, l-glycyl-phenylalanine by the three fractions it is suggested that the interaction of acid proteinases with the substrate involves hydrophobic forces. it has been shown that the above fractions are no metal enzymes. by means of the diazocarbon ... | 1975 | 1740 |
| preparative isolation of terrilytin components and study of their enzymatic properties. | by ion-exchange chromatography the composition of the fibrinolytic enzyme from aspergillus terricola-terrilytine-was studied. the preparation contained five components that differed in their substrate specificity. three of the components and proteolytic and fibrinolytic activities. the isolated fractions were examined with respect to their ph stability, ph optima (casein, hemoglobin and fibrin used as substrates), fibrinolytic and fibrinohenolytic activities. | 1976 | 11462 |
| cultivation conditions of the aspergillus terricola mutant producing proteolytic enzyme. | the effect of temperature, aeration and various foam suppressors on the growth of a mutant of aspergillus terricola h-20 and on its production of proteolytic enzymes was studied. a high level of the proteolytic activity (12-15 pu/ml) was found at 28-30 degree c in conditions of aeration with the rate of oxygen dissolution being 0.38-0.86 g of oxygen per litre per hour. synthesis of the enzyme was inhibited by a decrease in temperature to 22-24 degree c, a surplass (1.07 g o2 per litre per hour) ... | 1976 | 12453 |
| [effect of ph on the conformation properties and enzyme activity of alpha-amylase from aspergillus terricola]. | the effect of ph values on the conformation state of the protein globule and enzyme activity of alpha-amylase isolated from the culture liquid of the fungus aspergillus terricola was studied. by the method of dispersion of optical rotation, it was demonstrated that together with the disordered structure the alpha-amylase macromolecule in its native form contained alpha-helix and beta-structures. with a ph change the enzyme macromolecule showed two conformational transformations: with a ph decrea ... | 1978 | 34837 |
| [separation and characteristics of the acid proteinases from a surface culture of aspergillus terricola]. | the enzymic preparation isolated by ethanol precipitation from the aqueous extract of the surface culture of aspergillus terricola was purified on biogel p-10, fractionated on se-sephadex. as a result, 10 fractions of acid proteinases that differed in ph optima and activity were obtained. after purification specific activity of some fractions per 1 mg protein increased 24-32-fold. the temperature optimum of the enzymes was at 50 degrees c and ph optimum at 2.5--2.8. | 1975 | 241995 |
| [effectiveness of terrilytin in the treatment of experimental pulmonary vascular thrombosis]. | the thrombolytic effect of aspergillus terricola proteinase terrilytin was studied in 24-hour experimental pulmonary vascular thrombosis of rabbits. the drug was dissolved in polyvinylpyrrolidon and injected into the blood flow in doses of 175 and 220 pu/kg by two routes: locally (into the thrombosed vessel) and systemically. the drug produced a high thrombolytic effect irrespective of the administration and dosage; however, the effect was more marked in local infusion of terrilytin in a dose of ... | 1979 | 427272 |
| [population composition of aspergillus terricola, producer of terrilytin]. | | 1979 | 502916 |
| [direct selection of aspergillus terricola variants having different toxicity on a culture of fibroblasts]. | a direct method is proposed to select less toxic mutants of aspergillus terricola on a culture of fibroblasts. the conidia are first irradiated with uv, and then are used to grow colonies on the glass surface of tubes or flasks containing medium 199. the cells of fibroblasts are added thereupon and the two cultures are being grown together for 24--48 hours. the colonies which inhibit the growth of fibroblasts to a less extent are selected using microscopy. the method can be used for primarily se ... | 1977 | 600087 |
| [effect of inoculum material on biosynthesis of proteolytic and amylolytic enzymes of aspergillus tarricola]. | the effect of inoculation material on biosynthesis of proteolytic and amylolytic enzymes was studied with aspergillus terricola h-20. the highest yield of proteolytic enzymes was found with the inoculation material obtained on oatmeal flakes with single shaking of the substrate. the highest number of viable conidia was detected by the sixth day of the growth; they favoured the synthesis of both proteases and amylases. the maximum yield of proteases was found when the density of inoculation was 6 ... | 1977 | 895556 |
| [comparative cytomorphologic study of strains of aspergillus terricola in the process of biosynthesis of proteolytic enzymes]. | the cytology and morphology of three strains of aspergillus terricola were studied in the course of synthesis of proteolytic enzymes. the level of the enzymes in the cultural broth was highest during the growth of the diffused mycelium, and was accompanied with specific cytodifferentiation of the fungal hyphae. the contact between the hyphae of the producing culture and the growth medium is presumed to be important for biosynthesis of the enzymes. | 1977 | 895562 |
| some properties of the protease from aspergillus terricola. | the protease from aspergillus terricola (terrilytin) was isolated and purified by the ion exchange method and by gel chromatography. the data on its molecular weight, isoelectric point, n-terminal amino acids were described. the protease included polysaccharide which stabilized the enzyme activity under the storage and lyophylization. polysaccharides isolated from different fungi and yeasts were found to stabilize and activate terrilytin rising the affinity of enzyme to substrate. terrilytin was ... | 1975 | 1207716 |
| [component composition of the proteolytic complex synthesized by mutant aspergillus terricola strains]. | | 1986 | 2419376 |
| [proteolytic complex from aspergillus terricola (review)]. | results are presented of the studies on the proteolytic enzymes produced by the fungus aspergillus terricola that comprise the antiinflammatory drug terrilytin. terrilytin was shown to be a complex of three proteolytic enzymes that contained amylase as well. two of the proteases (proteases i and ii) were of the group of serine proteases by their physico-chemical and enzymatic characteristics and might be considered as two types of the same enzyme. protease iii showed characteristics specific for ... | 1986 | 2419893 |
| -glutamyl transpeptidase from aspergillus terricola. | | 1972 | 4144290 |
| [physicochemical and enzymatic properties of protease from aspergillus terricola]. | | 1972 | 4634390 |
| [proteinase biosynthesis by mutant strains of aspergillus terricola]. | | 1972 | 4655592 |
| [denaturation and autolysis of protease from aspergillus terricola]. | | 1973 | 4701625 |
| [purification of preparations of proteolytic enzymes from the surface of a culture of aspergillus terricola by the method of chromatography on biogel p-10]. | | 1973 | 4772154 |
| [biosynthesis of a proteolytic enzyme complex by aspergillus terricola mutants]. | | 1973 | 4790724 |
| [the influence of components of the medium on biosynthesis of a proteolytic enzyme by the fungus aspergillus terricola]. | | 1973 | 4799204 |
| [effect of medium composition on the synthesis of proteinase and amylase by aspergillus terricola 3-374]. | | 1973 | 4805935 |
| [optimization of composition and concentration of components of the culture medium for biosynthesis of proteinase by aspergillus terricola 3-374]. | | 1972 | 5044889 |
| [study of the proteinase of aspergillus terricola by electrophoresis]. | | 1969 | 5345732 |
| [isolation and properties of the proteinase of aspergillus terricola]. | | 1969 | 5345744 |
| [mutants of aspergillus terricola producing proteases with fibrinolytic activity]. | | 1969 | 5396575 |
| [biosynthesis of aspergillus terricola proteinase on media containing organic nitrogen compounds]. | | 1970 | 5490453 |
| [effect of carbohydrates on proteinase biosynthesis by aspergillus terricola in submerged cultures]. | | 1970 | 5493348 |
| [repression of protease synthesis in aspergillus terricola caused by exogenous amino acids]. | | 1971 | 5567623 |
| [growth media stimulating protease synthesis in aspergillus terricola and its mutants]. | | 1971 | 5580115 |
| [variability in the activity of proteolytic enzymes in aspergillus terricola, caused by the action of mutagenic factors]. | | 1971 | 5580124 |
| [effect of l-lysine on growth rate of aspergillus terricola]. | | 1967 | 5618179 |
| [analysis of the component constitution and substrate specificity of a fibrinolytic preparation from the fungus flammulina velutipes]. | the degree of heterogeneity of the proteolytic complex from the fungus flammulina velutipes was studied by gel chromatography and analytical isoelectrofocusing. the fibrinolytic, thrombolytic, caseinolytic, endo- and aminopeptidase activities of the enzyme complex were compared to those of aspergillus terricola and streptomyces griseus proteinases. the proteolytic complex under study consists of at least two proteinases with pi 6.1 and 7.1, which possess fibrinolytic, thrombolytic and endopeptid ... | 1982 | 7052148 |
| mode and extent of degradation of adenosine and guanosine by extracts of aspergillus terricola. | the mode of degradation of adenosine by extracts of aspergillus terricola was suggested to be affected preliminary by adenosine deaminase to inosine and the resulting ribonucleoside was then degraded hydrolytically to give hypoxanthine and ribose. with regard to guanosine, the same extracts could initially catalyze the hydrolytic cleavage of guanosine to guanine and ribose. the resulting base was then deaminated to give xanthine by guanine deaminase. addition of arsenate to the reaction mixture ... | 1995 | 7551735 |
| hydrolytic cleavage of purine ribonucleosides by extracts of aspergillus terricola. | cell-free extracts of nitrate-grown aspergillus terricola could catalyze the hydrolytic cleavage of the n-glycosidic bond of adenosine, guanosine and inosine optimally at ph 4 and 50 degrees c. incubation of the extracts at 60 degrees c for 60 minutes caused about 86%, 67% and 54% loss of activity respectively. the similarities between the ph or the temperatures profiles indicate that the hydrolytic cleavage of these purine ribonucleosides might be effected by one hydrolase. the results obtained ... | 1994 | 7740979 |
| properties of adenosine deaminase in extracts of asperigillus terricola. | cell-free extracts of nitrate-grown aspergillus terricola catalyze the hydrolytic deamination of adenosine to inosine at maximum rate at ph 6.5 and 50 degrees c. incubation of the extracts at 60 degrees c for 30 minutes caused about 66.7% loss in activity. results indicated the involvement of sh groups in the catalytic site of adenosine deaminase. frequent freezing and thawing of the enzyme preparation for three days (3 times) resulted in about 47% loss in activity. the enzyme is also inhibited ... | 1994 | 7740980 |
| crab collagenase in wound debridement. | a comparative study was performed of the caseinolytic, necrolytic and fibrinolytic activities of a crab collagenase product prepared from the hepatopancreas of the king crab (paralithodes camtschatica) and four enzyme preparations (trypsin/chymotrypsin and protease complexes isolated from aspergillus terricola, carica papaya and pseudomonodaceae). this paper reports an in vitro investigation, an in vivo study with rats and a clinical evaluation. it was found that crab collagenase has the highest ... | 1997 | 9117826 |
| [new method of obtaining high activity proteolytic ferments from aspergillus terricola]. | | 1952 | 13002134 |
| [comparative evaluation of a new culture medium from aspergillus terricola]. | | 1952 | 13010499 |
| [study of toxin formation of c1. perfringens on medium prepared by the method of hydrolysis of casein and fish bone meal with the aid of proteases from the fungus aspergillus terricola]. | | 1960 | 13730673 |
| isolation and evaluation of tannin-degrading fungal strains from the mexican desert. | eleven fungal strains (4 penicillium commune, 2 aspergillus niger, 2 aspergillus rugulosa, aspergillus terricola, aspergillus ornatus and aspergillus fumigatus) were isolated, characterized morphologically and by their capacity to degrade tannins. aspergillus niger aa-20 was used as control strain. several concentrations of hydrolysable tannin (tannic acid) were used as sole carbon source. all strains were able to degrade hydrolysable tannins. aspergillus niger gh1 and psh showed the highest tan ... | 2005 | 16402543 |
| enantioselective baeyer-villiger oxidation of bicyclo[3.2.0]hept-2-en-6-one with fungi: optimization of biotransformation and use of tio2 as support of cell growth. | fungi from amazonian forest soil (ecuador) and an italian factory were screened for baeyer-villiger (bv) oxidation of bicyclo [3.2.0]hept-2-en-6-one to 2-oxabicyclo[3.3.0]oct-6-en-3-one (corey's lactone). isolates of fusarium sp. and f. solani produced the (+)-(1r,5s)-lactone while isolates of aspergillus terricola and a. amazonicus afforded the (-)-(1s,5r)-lactone. highest conversions (85% yield and 70% enantiomeric excess) were obtained with a. amazonicus grown in presence of 2.7 mm titanium d ... | 2006 | 16786245 |
| purification and characterization of pectin lyase produced by aspergillus terricola and its application in retting of natural fibers. | an indigenously isolated fungal strain identified as aspergillus terricola with assigned fungal strain number mtcc 7588 has been used as source for pectin lyase production. the extracellular pectin lyase was purified to homogeneity from the culture filtrate of a. terricola by ion exchange and gel filtration chromatography. the determined molecular weight was 35 +/- 01 kda. the k(m) and k(cat) (turnover) values of the purified enzyme at 37 degrees c using citrus pectin as the substrate were found ... | 2009 | 19089648 |
| production and properties of xylanases from aspergillus terricola marchal and aspergillus ochraceus and their use in cellulose pulp bleaching. | aspergillus terricola and aspergillus ochraceus, isolated from brazilian soil, were cultivated in vogel and adams media supplemented with 20 different carbon sources, at 30 degrees c, under static conditions, for 120 and 144 h, respectively. high levels of cellulase-free xylanase were produced in birchwood or oat spelt xylan-media. wheat bran was the most favorable agricultural residue for xylanase production. maximum activity was obtained at 60 degrees c and ph 6.5 for a. terricola, and 65 degr ... | 2010 | 20091051 |
| purification and characterization of a novel thermostable mycelial lectin from aspergillus terricola. | lectin has been isolated from mycelia of aspergillus terricola by single step purification on porcine stomach mucin-sepharose 4b affinity column. lectin could be effectively purified with 75% recovery and 4.47-fold increase in specific activity. lectin migrated as a single band on sds-page with an apparent molecular mass of 32.5 kda. sugar inhibition assay revealed that the lectin did not strongly interact with most carbohydrates and their derivatives tested while strong binding affinity to d-gl ... | 2010 | 20108052 |
| production of xylanolytic enzymes by aspergillus terricola in stirred tank and airlift tower loop bioreactors. | fungi producing high xylanase levels have attracted considerable attention because of their potential industrial applications. batch cultivations of aspergillus terricola fungus were evaluated in stirred tank and airlift bioreactors, by using wheat bran particles suspended in the cultivation medium as substrate for xylanase and β-xylosidase production. in the stirred tank bioreactor, in physical conditions of 30°c, 300 rpm, and aeration of 1 vvm (1 l min(-1)), with direct inoculation of fungal s ... | 2011 | 21626207 |
| production of xylanase and β-xylosidase from autohydrolysis liquor of corncob using two fungal strains. | agroindustrial residues are materials often rich in cellulose and hemicellulose. the use of these substrates for the microbial production of enzymes of industrial interest is mainly due to their high availability associated with their low cost. in this work, corncob (ccs) particles decomposed to soluble compounds (liquor) were incorporated in the microbial growth medium through autohydrolysis, as a strategy to increase and undervalue xylanase and β-xylosidase production by aspergillus terricola ... | 2012 | 22367528 |
| crab collagenase in wound debridement. | a comparative study was performed of the caseinolytic, necrolytic and fibrinolytic activities of a crab collagenase product prepared from the hepatopancreas of the king crab (paralithodes camtschatica) and four enzyme preparations (trypsin/chymotrypsin and protease complexes isolated from aspergillus terricola, carica papaya and pseudomonodaceae). this paper reports an in vitro investigation, an in vivo study with rats and a clinical evaluation. it was found that crab collagenase has the highest ... | 1997 | 27937744 |
| effects of aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages. | cellulose and hemicellulose are quantitatively the most important structural carbohydrates present in ruminant diets. rumen micro-organisms produce enzymes that catalyse their hydrolysis, but the complex network formed by structural carbohydrates and lignin reduces their digestibility and restricts efficient utilisation of feeds by ruminants. this study aimed to produce two enzymatic extracts, apply them in ruminant diets to determine the best levels for ruminal digestibility and evaluate their ... | 2012 | 22508186 |