| striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses. | the sequence of the 3'-terminal 2956 nucleotides, excluding the poly(a) tail, of the citrus tatter leaf virus (ctlv) genome was determined and compared with that of the apple stem grooving virus (asgv) genome. the sequence of the 3'-terminal region of ctlv contains two overlapping open reading frames (orfs) and a 3'-terminal non-coding region of 142 nucleotides. the long, incomplete orf1 ends at uag (position 2812) and encodes a protein with at least 938 amino acids (m(r) > 108,703). this protei ... | 1993 | 8277280 |
| tepovirus, a novel genus in the family betaflexiviridae. | tepovirus is a new monotypic genus of plant viruses typified by potato virus t (pvt), a virus with helically constructed filamentous particles that are 640 nm long, previously classified as unassigned species in the family betaflexiviridae. virions have a single-stranded positive-sense polyadenylated rna genome that is 6.5 kb in size, and a single type of coat protein with a size of 24 kda. the viral genome contains three slightly overlapping orfs encoding, respectively, the replication-related ... | 2012 | 22592959 |
| characterization by deep sequencing of prunus virus t, a novel tepovirus infecting prunus species. | double-stranded rnas purified from a cherry tree collected in italy and a plum tree collected in azerbaijan were submitted to deep sequencing. contigs showing weak but significant identity with various members of the family betaflexiviridae were reconstructed. sequence comparisons led to the conclusion that the viral isolates identified in the analyzed prunus plants belong to the same viral species. their genome organization is similar to that of some members of the family betaflexiviridae, with ... | 2015 | 25054616 |
| infection of capilloviruses requires subgenomic rnas whose transcription is controlled by promoter-like sequences conserved among flexiviruses. | the first open-reading frame (orf) of apple stem grooving virus (asgv), of the genus capillovirus, encodes an apparently chimeric polyprotein containing conserved regions for replicase (rep) and coat protein (cp). however, our previous study revealed that asgv mutants with distinct and discontinuous rep- and cp-coding regions successfully infect plants, indicating that cp expressed via a subgenomic rna (sgrna) is sufficient for viability of the virus. here we identified a transcription start sit ... | 2012 | 22401846 |
| the complete nucleotide sequence of potato virus t. | potato virus t (pvt), a member of an unassigned species in the family flexiviridae, has a genome 6,539 nt in size with three orfs coding for replication-associated proteins (185 kda, orf 1), movement protein (40 kda, orf 2) and coat protein (24 kda, orf 3), respectively. pvt differs from the type members of all genera of the family flexiviridae with a 30k-type movement protein and is phylogenetically distant from all of these viruses, least so from grapevine virus a (gva, genus vitivirus), with ... | 2009 | 19115033 |