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identity of naegleria strains isolated from organs of freshwater fishes.eighteen naegleria strains were isolated from organs of freshwater fishes belonging to 5 species. morphometric study allowed the separation of the naegleria strains from the non-vahlkampfiid amoeboflagellates, but was inadequate for species determination. six strains, representatives of groups that had a slightly different cyst size, were selected and corresponding derived clones were subjected to sequence analysis and riboprinting restriction fragment length polymorphism (rflp)-pcr analysis of ...200111678226
application of taqman qpcr for the detection and monitoring of naegleria species in reservoirs used as a source for drinking water.naegleria spp. can be found in the natural aquatic environments. naegleria fowleri can cause fatal infections in the central nervous system in humans and animals, and the most important source of infection is through direct water contact. in this study, pcr of 5.8s ribosomal rna (rrna) gene and internal transcribed spacer (its) region was performed in order to identify naegleria isolates and quantify the naegleria spp. by taqman real-time quantitative pcr in reservoir water samples. the occurren ...201425079704
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