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effects of different vertebrate growth factors on primary cultures of hemocytes from the gastropod mollusc, haliotis tuberculata.a useful experimental system from primary cultures of hemocytes from haliotis tuberculata has been established. six days after initiation of the culture, the viability of hemocytes remained constant as measured by the mtt assay. in addition, hemocytes showed physiological responses as judged by protein and dna syntheses in response to treatment with vertebrate growth factors. porcine insulin and human epidermal growth factor (egf) stimulated [3h]-leucine and [3h]-thymidine incorporation in hemoc ...19968688833
nmr spectroscopy analysis of oligoguluronates and oligomannuronates prepared by acid or enzymatic hydrolysis of homopolymeric blocks of alginic acid. application to the determination of the substrate specificity of haliotis tuberculata alginate lyase.the 1h and 13c nmr chemicals shifts of the various saturated and unsaturated timers obtained by acid or enzymatic depolymerisation of homopolymeric blocks of alginates are reported. in addition, 13c nmr chemical shifts are assigned for several saturated oligomers of higher polymerisation degrees. breakdown of alginate and of homopolymeric alginate blocks by haliotis tuberculata alginate lyase was monitored with 1h nmr spectroscopy and the signals relevant to the identification of the lyase produ ...19968805773
characterization of a calcitonin gene related peptide-like molecule in the abalone, haliotis tuberculata.immunoreactive related cgrp molecules (ir-cgrp) were identified in the abalone, haliotis tuberculata, mainly in mantle and cephalic part extracts. ir-cgrp in both tissues accounted for 461 and 455.6 pg per mg of proteins, respectively. these cgrp-immunoreactive molecules were further analyzed for their ability to interact with the cgrp radioreceptor assay. in specific target tissues for cgrp (rat liver membranes), 50% inhibition of 125i-labeled cgrp specific binding was observed with 4.7 microgr ...19979134700
abalone (haliotis tuberculata) hemocyanin type 1 (hth1). organization of the approximately 400 kda subunit, and amino acid sequence of its functional units f, g and h.we have identified two separate hemocyanin types (hth1 and hth2) in the european abalone haliotis tuberculata. hth1/hth2 hybrid molecules were not found. by selective dissociation of hth2 we isolated hth1 which, as revealed by electron microscopy and sds/page, is present as didecamers of a approximately 400 kda subunit. immunologically, hth1 and hth2 correspond to keyhole limpet hemocyanin (klh)1 and klh2, respectively, the two well-studied hemocyanin types of the closely related marine gastropo ...199910447670
collagen study and regulation of the de novo synthesis by igf-i in hemocytes from the gastropod mollusc, haliotis tuberculata.to evidence a collagen synthesis and identify which type(s) of collagen is present in hemocytes from the mollusc haliotis tuberculata, we have performed three separate approaches, namely, de novo synthesis by cultured cells, immunological approaches, and northern blot analysis. we demonstrated first that after 40-hr labeling, the de novo synthesis of collagen in the cell layer of cultured hemocytes represents 9.48 +/- 1.25% with respect to the total [(3)h]proline-labeled protein synthesis. in ad ...200010951387
dietary effects of marine food intake on intestinal and hepatic enzyme activities in rats.dietary effects of two diets high in protein from two marine species (haliotis tuberculata and anemonia viridis) as compared to a high-quality patron protein such as casein (or casein supplemented with olive oil) on intestinal and hepatic enzymes were studied. after 23 days, the two marine species as diet compared to casein increased the disaccharidase and alkaline phosphatase activities. feeding haliotis tuberculata meal produced a decrease on intestinal leucine aminopeptidase activity. the hep ...200111339110
nutritional value of the marine invertebrates anemonia viridis and haliothis tuberculata and effects on serum cholesterol concentration in ratsopen starthe purpose of this study was to determine the nutritional value of diets with protein from two marine species (haliotis tuberculata and anemonia viridis) as compared to a high-quality protein reference based on casein or casein supplemented with olive oil. we also investigated the effects of these diets on serum lipid levels. male rats were fed these diets for 23 days. protein quality indicators (true digestibility, net protein utilization, biological value) were similar to those obtained for c ...200111834211
amino acid sequence and glycosylation of functional unit rth2-e from rapana thomasiana (gastropod) hemocyanin.the complete amino acid sequence of rapana thomasiana hemocyanin functional unit rth2-e was determined by direct sequencing and matrix-assisted laser desorption ionization mass spectrometry of peptides obtained by cleavage with endolysc proteinase, chymotrypsin, and trypsin. the single-polypeptide chain of rth2-e consists of 413 amino acid residues and contains two consensus sequences nxs/t (positions 11-19 and 127-129), potential sites for n-glycosylation. monosaccharide analysis of rth2-e reve ...200211888200
vibrio carchariae, a pathogen of the abalone haliotis tuberculata.since 1997, mass mortality of the abalone haliotis tuberculata l. has occurred in the natural environment along the french coast. the outbreak of disease started on the south coast of brittany near concarneau in 1997, then spread to the north of brittany (in 1998) and the west coast of normandy (golfe de st. malo in 1999). between 60 and 80% of the abalone died. in 1999, mortality also affected a land-based abalone farm in normandy during the summer. at this farm, a vibrio sp. was isolated in ab ...200212152903
gene structure and hemocyanin isoform hth2 from the mollusc haliotis tuberculata indicate early and late intron hot spots.we have cloned and sequenced cdnas coding for the complete primary structure of hth2, the second hemocyanin isoform of the marine gastropod haliotis tuberculata. the deduced protein sequence comprises 3399 amino acids, corresponding to a molecular mass of 392 kda. it shares only 66% of structural identity with the previously analysed first isoform hth1, and according to a molecular clock, the two isoforms of haliotis hemocyanin separated ca. 320 million years ago. by genomic polymerase chain rea ...200212490323
vibrio gallicus sp. nov., isolated from the gut of the french abalone haliotis tuberculata.five alginolytic, facultatively anaerobic, non-motile bacteria were isolated from the gut of the abalone haliotis tuberculata. phylogenetic analyses based on 16s rdna data indicated that these strains are related to vibrio wodanis, vibrio salmonicida, vibrio logei and vibrio fischeri (but with <97 % 16s rrna gene sequence similarity). dna-dna hybridization and fluorescence amplified fragment length polymorphism fingerprinting demonstrated that the five strains constituted a single species that w ...200415143033
ultrastructural and molecular characterization of haplosporidium montforti n. sp., parasite of the european abalone haliotis tuberculata.a new member of the parasitic phylum haplosporidia, which was found infecting the connective tissue, gill, digestive gland, and foot muscle of haliotis tuberculata imported from ireland and experimentally grown in galicia (nw spain), is described. scanning electron microscopy, transmission electron microscopy, and molecular characterization of the small subunit ribosomal rna (ssu rrna) gene were carried out to confirm the description of this species. the ultrastructural morphology of the spores ...200616563428
shell disease: abnormal conchiolin deposit in the abalone haliotis tuberculata.shell disease in the abalone haliotis tuberculata l. is characterized by a conchiolin deposit on the inner surface of the shell. the gross clinical signs appear similar to the brown ring disease (brd) of clams. brd has been extensively described in clams and is known to be responsible for severe mortalities and the collapse of the clam aquaculture industry in western france. in the clam, it was found to be caused by the infection of the mantle by vibrio tapetis. brown protein deposits have been ...200616610593
ultrastructural description of new rickettsia-like organisms in the commercial abalone haliotis tuberculata (gastropoda: haliotidae) from the nw of spain.rickettsia-like organisms (rlos) were found in the commercially farmed abalone haliotis tuberculata in the northwestern region of the atlantic coast of spain and are described from light and transmission electron microscopy observations. the rlos measured approximately 1.6 x 0.9 microm and were found in intracytoplasmic, spherical to ellipsoidal vacuoles (up to 8 microm) in the epithelial cells of the digestive diverticulae. the morphological ultrastructure of these organisms was typically proka ...200617058604
variations of 210po and 210pb in various marine organisms from western english channel: contribution of 210po to the radiation dose.measurements of (210)po were carried out in various marine matrices (mussels, oysters, seaweed, fish, and abalones) and in seawater at several points along the french coast, over a period of 2 years (2003-2005). these measurements contribute to a better knowledge of this element, since few recent data exist for the french coast. marked seasonal variations have been revealed in some species and there are differences according to the way of life of these species. activities in mussels (mytilus edu ...200717566617
summer immune depression associated with increased susceptibility of the european abalone, haliotis tuberculata to vibrio harveyi infection.haliotis tuberculata mortality outbreaks have occurred in france since 1998 and were attributed to a pathogenic vibrio harveyi. these mortalities were recorded in september, a month with abalone reproduction and characterised by high seawater temperatures. the importance of gonadal maturation and temperature increase on abalone immunity and susceptibility to v. harveyi infection needed to be clarified. therefore, an immune survey analyzing a large panel of parameters was performed from june to s ...200818786640
mitochondrial dna introgression in the european abalone haliotis tuberculata tuberculata: evidence for experimental mtdna paternal inheritance and a natural hybrid sequence.two subspecies of the european abalone have been morphologically recognized: haliotis tuberculata tuberculata, present in the north atlantic, and haliotis tuberculata coccinea, present in the canary islands. among the different nuclear markers used to differentiate these two subspecies, the sperm lysin gene was the most reliable, leading to a 2.2% divergence. concerning the subunit i of the mitochondrial cytochrome oxydase gene (coi), we observed a difference of 3.3% between the two subspecies. ...201020938796
characterization of a non-fibrillar-related collagen in the mollusc haliotis tuberculata and its biological activity on human dermal fibroblasts.in invertebrates, members of the collagen family have been found in various phyla. surprisingly, in mollusc, little is known about such molecules. in this study, we characterize the full-length abalone type iv collagen and we analysed its biological effects on human fibroblast in order to gain insights about this molecule in molluscs and particularly clues about its roles. we screened a cdna library of haliotis tuberculata hemocytes. the expression pattern of the transcript is determined using r ...201121271271
permanent genetic resources added to the molecular ecology resources database 1 february 2010-31 march 2010.this article documents the addition of 228 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: anser cygnoides, apodemus flavicollis, athene noctua, cercis canadensis, glis glis, gubernatrix cristata, haliotis tuberculata, helianthus maximiliani, laricobius nigrinus, laricobius rubidus, neoheligmonella granjoni, nephrops norvegicus, oenanthe javanica, paramuricea clavata, pyrrhura orcesi and samanea saman. these loci were cross-t ...201021565086
proteomic strategy for identifying mollusc shell proteins using mild chemical degradation and trypsin digestion of insoluble organic shell matrix: a pilot study on haliotis tuberculata.a successful strategy for the identification of shell proteins is based on proteomic analyses where soluble and insoluble fractions isolated from organic shell matrix are digested with trypsin with the aim of generating peptides, which are used to identify novel shell proteins contained in databases. however, using trypsin as a sole degradative agent is limited by the enzyme's cleavage specificity and is dependent upon the occurrence of lysine and arginine in the shell protein sequence. to bypas ...201122160345
structures of two molluscan hemocyanin genes: significance for gene evolution.we present here the description of genes coding for molluscan hemocyanins. two distantly related mollusks, haliotis tuberculata and octopus dofleini, were studied. the typical architecture of a molluscan hemocyanin subunit, which is a string of seven or eight globular functional units (fus, designated a to h, about 50 kda each), is reflected by the gene organization: a series of eight structurally related coding regions in haliotis, corresponding to fu-a to fu-h, with seven highly variable linke ...200111287637
acute toxicity of 8 antidepressants: what are their modes of action?currently, the hazard posed by pharmaceutical residues is a major concern of ecotoxicology. most of the antidepressants belong to a family named the cationic amphipathic drugs known to have specific interactions with cell membranes. the present study assessed the impact of eight antidepressants belonging to selective serotonin reuptake inhibitors or serotonin norepinephrine reuptake inhibitors by the combination of multi-approaches (in vivo, in vitro, in silico) and gives some insights on the mo ...201424534154
characterization of abalone haliotis tuberculata-vibrio harveyi interactions in gill primary cultures.the decline of european abalone haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus vibrio. following the summer mortality outbreaks reported in france between 1998 and 2000, vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone h. tuberculata and v. harveyi. this work reports the development of primary cell cultures from abalone gill tissue, a target tissue fo ...201323756730
the impacts of handling and air exposure on immune parameters, gene expression, and susceptibility to vibriosis of european abalone haliotis tuberculata.wild or farmed abalone are regularly exposed to stressors, such as air exposure and handling. immune and transcriptional responses as well as susceptibility to vibriosis of sexually mature or immature european abalone acclimated at 16 or 19 °c were determined following handling or air exposure. hemocyte density and h2o2 production increased while hemocyte viability and phagocytic index decreased following handling. air exposure induces a decrease of hemocyte density and phagocytic index. measure ...201424215911
vibrio harveyi adheres to and penetrates tissues of the european abalone haliotis tuberculata within the first hours of contact.vibrio harveyi is a marine bacterial pathogen responsible for episodic epidemics generally associated with massive mortalities in many marine organisms, including the european abalone haliotis tuberculata. the aim of this study was to identify the portal of entry and the dynamics of infection of v. harveyi in the european abalone. the results indicate that the duration of contact between v. harveyi and the european abalone influences the mortality rate and precocity. immediately after contact, t ...201425107972
the early stages of the immune response of the european abalone haliotis tuberculata to a vibrio harveyi infection.vibrio harveyi is a marine bacterial pathogen responsible for episodic abalone mortalities in france, japan and australia. in the european abalone, v. harveyi invades the circulatory system in a few hours after exposure and is lethal after 2 days of infection. in this study, we investigated the responses of european abalone immune cells over the first 24 h of infection. results revealed an initial induction of immune gene expression including rel/nf-kb, mpeg and clathrin. it is rapidly followed ...201525766281
de novo assembly and annotation of the european abalone haliotis tuberculata transcriptome.the european abalone haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic vibrio infection. transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. to g ...201626971316
immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the european abalone.since 1997, populations of the european abalone haliotis tuberculata suffer mass mortalities attributed to the bacterium vibrio harveyi. these mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °c, leading to favorable conditions for v. harveyi proliferation. in order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct brittany populations: one that has ...201727836724
gene expression patterns of abalone, haliotis tuberculata, during successive infections by the pathogen vibrio harveyi.since 1998, episodic mass mortality of the abalone haliotistuberculata has been observed along the northern brittany coast of france caused by a complex interaction among the host, pathogen and environmental factors. in the present study, abalone were submitted to two successive infections with the pathogen vibrioharveyi under controlled conditions. during the first challenge, infection by v.harveyi resulted in 64% mortality of mature abalone. after a second infection of those surviving the firs ...201020692263
molecular characterization and induction of heat shock protein 90 in the antarctic bivalve laternula elliptica.heat shock protein 90 (hsp90) is a highly conserved molecular chaperone that plays a key role in protein synthesis, folding, denaturation prevention, and signal transduction. we cloned the complete complementary dna (cdna) sequence of the laternula elliptica hsp90. the full-length cdna was 2,823 bp in size and contained an open reading frame of 2,190 bp that was translated into 729 amino acids with a calculated molecular weight of 83.4 kda. the deduced amino acid sequence of hsp90 showed the hig ...200918987993
ultrastructural developmental cycle of haplosporidium montforti (phylum haplosporidia) in its farmed abalone host, haliotis tuberculata (gastropoda).the sequential developmental cycle of haplosporidium montforti, a recently described species from farmed abalone haliotis tuberculata (gastropoda), was studied. ornamented and operculated mature spores were electron dense. the nucleus of the uninucleated free cell divided successively, giving rise to multinucleate plasmodia, containing up to 100-120 nuclei. later, the plasmodia developed into sporonts inside sporocysts with irregular contours. each of their nuclei gave rise to uninucleate sporob ...200818372632
construction of a stable gfp-tagged vibrio harveyi strain for bacterial dynamics analysis of abalone infection.vibrio harveyi is a bacterial marine pathogen that can cause fatal disease in a large range of vertebrates and invertebrates, including the commercially important marine gastropod, haliotis tuberculata. since 1997, strains of this bacterium have regularly been causing high mortalities in farmed and wild abalone populations. the way in which the pathogen enters into abalone and the disease transmission mechanisms are thus far unknown. therefore, a pathogenic strain, orm4, was green fluorescent pr ...200819054091
comparison of the sensitivity of seven marine and freshwater bioassays as regards antidepressant toxicity assessment.the hazards linked to pharmaceutical residues like antidepressants are currently a major concern of ecotoxicology because they may have adverse effects on non-target aquatic organisms. our study assesses the ecotoxicity of three antidepressants (fluoxetine, sertraline and clomipramine) using a battery of marine and freshwater species representing different trophic levels, and compares the bioassay sensitivity levels. we selected the following bioassays: the algal growth inhibition test (skeleton ...201425185786
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