| a myoglobin evolved from indoleamine 2,3-dioxygenase. | hemoglobins and myoglobins are some of the best studied proteins. they are distributed in animals, plants and bacteria, and the characteristic two intron-three exon structure is widely conserved in animal globin genes (jhiang et al., 1988). to date, all of the hemoglobins and myoglobins are believed to have a common origin, and so they are considered to be homologous. we have isolated a completely new type of myoglobin from the red muscle of the abalone sulculus diversicolor aquatilis. the myogl ... | 1992 | 1453473 |
| the ear-shell (sulculus diversicolor aquatilis) myoglobin is composed of an unusual 39 kda polypeptide chain. | an unusual myoglobin was isolated from the buccal mass of the ear-shell sulculus diversicolor aquatilis. the myoglobin consists of a 39 kda polypeptide chain which is about double the size of the usual myoglobin subunit, contains one heme per molecule, and has an unusual spectral property ion the oxy-form. on the basis of these properties and partial amino acid sequencing, we propose that sulculus myoglobin has a didomain structure, and that one of the two domains does not function as an oxygen- ... | 1989 | 2806465 |
| abalone myoglobins evolved from indoleamine dioxygenase: the cdna-derived amino acid sequence of myoglobin from nordotis madaka. | the cdna for the unusual 41 kd myoglobin of the abalone nordotis madaka was amplified by polymerase chain reaction (pcr), and the cdna-derived amino acid sequence of 378 residues was determined. as with the myoglobin of the related abalone sulculus diversicolor (suzuki and takagi, j. mol. biol. 228, 698-700, 1992), the sequence of nordotis myoglobin showed no significant homology with any other globins, but showed high homology (35% identity) with vertebrate indoleamine 2,3-dioxygenase, a trypto ... | 1994 | 8011076 |
| convergent evolution. the gene structure of sulculus 41 kda myoglobin is homologous with that of human indoleamine dioxygenase. | the abalone sulculus diversicolor contains abundant myoglobin in its buccal mass. the myoglobin consists of 377 amino acid residues and has a molecular mass of 41 000 da, 2.5 times larger than that of other myoglobins. sulculus myoglobin can bind oxygen reversibly, and the p50 was determined to be 3.8 mmhg at 20 degrees c and ph 7.4, showing that the oxygen affinity of sulculus myoglobin is lower than those of vertebrate and invertebrate myoglobins. the cdna-derived amino acid sequence showed no ... | 1996 | 8765749 |
| comparative studies of the indoleamine dioxygenase-like myoglobin from the abalone sulculus diversicolor. | the abalone sulculus diversicolor contains abundant myoglobin in its buccal mass. the myoglobin is homodimeric and the molecular mass of the constituent polypeptide chain is 41,000 da. the amino acid sequence and gene structure are highly homologous with those of a vertebrate tryptophan-degrading enzyme, indoleamine dioxygenase (ido). thus sulculus myoglobin evolved from an ido gene, and represents a typical case of functional convergence. the oxygen equilibrium properties of sulculus myoglobin ... | 1997 | 9297802 |
| amino acid sequence, spectral, oxygen-binding, and autoxidation properties of indoleamine dioxygenase-like myoglobin from the gastropod mollusc turbo cornutus. | myoglobin was isolated from the radular muscle of the archaeogastropod mollusc turbo cornutus (turbinidae). this myoglobin is a monomer carrying one protoheme group; the molecular mass was estimated by sds-page to be about 40 kda, 2.5 times larger than that of usual myoglobin. the cdna-derived amino acid sequence of 375 residues was determined, of which 327 residues were identified directly by chemical sequencing of internal peptides. the amino acid sequence of turbo myoglobin showed no signific ... | 1998 | 9988528 |
| lethal attribute of serine protease secreted by vibrio alginolyticus strains in kuruma prawn penaeus japonicus. | toxicity of the extracellular products (ecp) and the lethal attribute of serine protease secreted by five pathogenic vibrio alginolyticus strains from various sources in kuruma prawn penaeus japonicus were studied. the ecps of organisms originally isolated from diseased kuruma prawn or small abalone haliotis diversicolor supertexta were more lethal (ld50 value of 0.48 or 0.41 microg protein/g prawn) than those from diseased tiger prawn p. monodon, yellowfin porgy acanthopagrus latus or horse mac ... | 2000 | 10739107 |
| virulence of vibrio parahaemolyticus isolated from cultured small abalone, haliotis diversicolor supertexta, with withering syndrome. | outbreaks of mass mortality among cultured small abalone haliotis diversicolor supertexta with withering syndrome occurred in may and september 1998 in kao-hsiung, taiwan. bacterial strains ch-1 and b4 were isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% nacl and/or thiosulphate citrate bile salt sucrose agar. these two strains were characterized and identified as vibrio parahaemolyticus on the basis of various biochemical tests. the b4 stra ... | 2000 | 11123551 |
| tropomyosin is the major mollusk allergen: reverse transcriptase polymerase chain reaction, expression and ige reactivity. | the complementary dnas encoding tropomyosins of the abalone haliotis diversicolor, the scallop chlamys nobilis, and the mussel perna viridis were amplified by reverse transcriptase polymerase chain reaction and thereafter cloned in plasmid vectors for expression. immunoblot analysis showed that recombinant proteins of abalone, scallop, and mussel tropomyosin were reactive to serum ige antibodies from subjects allergic to shellfish but not to nonallergic controls. nucleotide and amino acid sequen ... | 2000 | 11246417 |
| pathogenicity of vibrio alginolyticus isolated from diseased small abalone haliotis diversicolor supertexta. | outbreaks of mass mortality among cultured small abalone haliotis diversicolor supertexta with abscess/ulcers in the mantle occurred in 1998 at kao-hsiung, taiwan. a swarming bacterium, strain h-11 was isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% nacl and/or thiosulphate citrate bile salt sucrose agar. this strain was characterized and identified as vibrio alginolyticus on the basis of various biochemical tests. the h-11 strain and its ex ... | 2001 | 11297013 |
| toxicokinetics and acute toxicity of waterborne zinc in abalone (haliotis diversicolor supertexta lischke). | | 2001 | 11443329 |
| withering syndrome of the small abalone, haliotis diversicolor supertexta, is caused by vibrio parahaemolyticus and associated with thermal induction. | the susceptibility of the small abalone haliotis diversicolor supertexta to vibrio parahaemolyticus 880915 strain and its extracellular products (ecp) at different temperatures was investigated. the strain was previously isolated from the haemolymph of the moribund small abalone with withering syndrome during an outbreak of mass mortality among the cultured animals in september 1999 in i-lan, taiwan. the bacterium and its ecp were lethal to the small abalone. onset of the withering syndrome in t ... | 2001 | 11724402 |
| vibrio carchariae, a pathogen of the abalone haliotis tuberculata. | since 1997, mass mortality of the abalone haliotis tuberculata l. has occurred in the natural environment along the french coast. the outbreak of disease started on the south coast of brittany near concarneau in 1997, then spread to the north of brittany (in 1998) and the west coast of normandy (golfe de st. malo in 1999). between 60 and 80% of the abalone died. in 1999, mortality also affected a land-based abalone farm in normandy during the summer. at this farm, a vibrio sp. was isolated in ab ... | 2002 | 12152903 |
| coupling toxicokinetics and pharmacodynamics for predicting survival of abalone (haliotis diversicolor supertexta) exposed to waterborne zinc. | we developed a mortality model, by coupling an acute toxicity model and a pharmacodynamic model, to predict survival of abalone (haliotis diversicolor supertexta) exposed to waterborne zinc (zn). we conducted a laboratory 14-day exposure experiment to obtain biokinetic parameters of depuration rate constant (k(2)) and bioconcentration factor (bcf). a one-compartment uptake-depuration model was used to fit the exposure data to estimate bcf and k(2) values. the acute toxicity model was developed b ... | 2002 | 12242679 |
| semen cryopreservation of small abalone (haliotis diversicolor supertexa). | methods for cryopreserving spermatozoa and maximizing fertilization rate in taiwan small abalone, haliotis diversicolor supertexa, were developed. the gametes (spermatozoa and eggs) of small abalone were viable 3 h post-spawning, with fertilization, and development rate decreasing with time. a minimum of 10(2) cell/ml sperm concentration and a contact time of 2 min between gametes is recommended for artificial insemination of small abalone eggs. eight cryoprotectants, dimethyl sulfoxide (dmso), ... | 2002 | 12374126 |
| linking biokinetics and consumer-resource dynamics of zinc accumulation in pond abalone haliotis diversicolor supertexta. | a dynamic model that links biokinetics and consumer-resource dynamics for describing zinc (zn) accumulation in abalone haliotis diversicolor supertexta has been developed and then applied to zn data from real abalone farms. the biokinetic parameters used in this study, uptake and depuration rate constants of abalone and their food source, red alga gracilaria tenuistipitata var. liui, were obtained from a laboratory 14-d exposure experiment. we carried out a sensitivity analysis of the model by u ... | 2002 | 12448558 |
| purification and characterization of amylases from small abalone (sulculus diversicolor aquatilis). | amylases ii-1 and ii-2 with molecular weights of 55.7 and 65 kda, respectively, were purified to electrophoretical homogeneity from small abalone (sulculus diversicolor aquatilis) by ammonium sulfate fractionation, sepharose cl-6b, cm-sepharose cl-6b, and sephacryl s-100 chromatographs. they had optimal temperatures of 45 and 50 degrees c and an optimal ph of 6.0. the purified amylases were stable at ph 5.0-8.0 and 6.0-8.0, respectively. they were completely or partially inhibited by hg(2+), cu( ... | 2003 | 12568573 |
| vibrio parahaemolyticus infectious for both humans and edible mollusk abalone. | the aims of this study are to report evidence of the first laboratory-acquired infection of vibrio parahaemolyticus associated with handling experimentally infected abalones and to describe the virulence of the two bacterial strains tested in these animals. two strains of v. parahaemolyticus, one from the stool of a patient with acute gastroenteritis (strain 880713) and the other from the hemolymph of a diseased small abalone haliotis diversicolor supertexta (strain 880915), were identified and ... | 2003 | 12758276 |
| probabilistic risk assessment of abalone haliotis diversicolor supertexta exposed to waterborne zinc. | this paper describes a risk assessment approach that integrates predicted tissue concentrations of zinc (zn) with a concentration-response relationship and leads to predictions of survival risk for pond abalone haliotis diversicolor supertexta as well as to the uncertainties associated with these predictions. the models implemented include a probabilistic bioaccumulation model, which linking biokinetic and consumer-resource models, accounts for zn exposure profile and a modified hill model for r ... | 2004 | 14568721 |
| shell of abalone haliotis diversicolor supertexta can eliminate waterborne zinc biokinetically. | dietary and nondietary accumulation of waterborne zinc (zn) by shell of abalone haliotis diversicolor supertexta was studied to determine if abalone shell can accumulate and eliminate zn biokinetically. shell of h. diversicolor supertexta rapidly accumulated zn at microgram per gram concentrations during a 7-d uptake period for dietary and nondietary exposure to 1 microgml(-1) zn seawater. depuration half-lives were 7.22 and 15.40 d for dietary and nondietary exposure, respectively. the uptake r ... | 2004 | 14592739 |
| growth toxicity bioassays of abalone haliotis diversicolor supertexta exposed to waterborne zinc. | | 2004 | 15058656 |
| the immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus at different salinity levels. | addition of nacl at 2.5% to 3.5% to tryptic soy broth (tsb) significantly increased the growth of vibrio parahaemolyticus. taiwan abalone haliotis diversicolor supertexta held in 30 per thousand seawater were injected with v. parahaemolyticus grown in tsb containing nacl at 0.5, 1.5, 2.5, 3.5 and 4.5% at a dose of 1.6 x 10(5)colony-forming units (cfu) abalone(-1). after 48 h, the cumulative mortality was significantly higher for the abalone challenged with v. parahaemolyticus grown in 2.5% than ... | 2004 | 15123299 |
| effect of ammonia on the immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus. | taiwan abalone haliotis diversicolor supertexta held in 30 parts/per thousand seawater and 26 degrees c were injected with tsb-grown vibrio parahaemolyticus (1.6 x 10(5)cfu abalone(-1)), and then placed in water containing different concentrations of ammonia-n (un-ionized plus ionized ammonia) at 0.01 mg l(-1) (control), 1.12, 3.22, 5.24 and 10.18 mg l(-1). mortality of abalone increased directly with ambient ammonia-n concentration. after 12 h, the mortality of v. parahaemolyticus-injected abal ... | 2004 | 15276599 |
| change in water temperature on the immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus. | taiwan abalones, haliotis diversicolor supertexta, held in 30 parts/per thousand seawater at 28 degrees c, were injected with tsb-grown vibrio parahaemolyticus (1.6x10(5) cfu abalone(-1)) and then transferred to 20, 24, 28 and 32 degrees c. all abalones transferred to 32 degrees c died by 72 h. the mortality of v. parahaemolyticus-injected abalone held at 20 and 24 degrees c was significantly lower over 24-96 h, compared to animals held at 28 and 32 degrees c. in a separate experiment designed t ... | 2004 | 15276603 |
| effect of nitrite on immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus. | taiwan abalones haliotis diversicolor supertexta held in 30% per thousand seawater and 26 degrees c were injected with tryptic soy broth (tsb)-grown vibrioparahaemolyticus (1.6 x 10(5) cfu [colony-forming units] abalone(-1)), and then placed in water containing different concentrations of nitrite-n (nitrite as nitrogen): 0.01 mg l(-1) (control), 1.05, 3.04, 5.10 and 10.06 mg l(-1). mortality of the abalones increased in direct parallel to ambient nitrite-n concentration. over 12 to 48 h, the mor ... | 2004 | 15460860 |
| do molluscs possess indoleamine 2,3-dioxygenase? | indoleamine 2,3-dioxygenase (ido)-like myoglobin (mb) was discovered in 1989 in the buccal mass of the abalone sulculus diversicolor, and it has since been isolated from several archaegastropods. the amino acid sequences and genomic structures of ido-like mbs show significant homology with those of mammalian idos, suggesting that they have evolved from a common ancestral gene. however, details of the evolutionary relationships between them remain unknown. here, we isolated a novel multicopy gene ... | 2005 | 15694593 |
| predictive risk thresholds for survival protection of farmed abalone, haliotis diversicolor supertexta, exposed to waterborne zinc. | using a probabilistic risk-based framework, we have developed a simple predictive risk threshold model for protecting the survival of farmed abalone, haliotis diversicolor supertexta, exposed to waterborne zinc (zn). probabilistic techniques using monte carlo analysis propagate parameter uncertainty/variability throughout the model, providing decision makers with a credible range of information and increased flexibility in establishing a specific zn level in aquacultural ecosystems. we coupled a ... | 2005 | 15793814 |
| herpes-like virus infection causing mortality of cultured abalone haliotis diversicolor supertexta in taiwan. | a herpes-like virus is demonstrated for the first time to be associated with high mortality rates in maricultured abalone haliotis diversicolor supertexta in taiwan. histopathology of moribund abalone indicated that the nerve system was the primary target tissue. the lesions were characterised by tissue necrosis accompanied with infiltration of haemocytes. electron microscopic examination demonstrated viral particles within the degenerated cerebral ganglion cells. the viruses were hexagonal, app ... | 2005 | 16042040 |
| [studies on extracellular virulent factors produced by potential pathogenic bacteria isolated from abalone postlarvae and biofilms]. | to find out the potential pathogen(s) that caused massive death of abalone postlarvae( haliotis diversicolor supertesta ) in southern china, 105 bacterial strains were isolated from the water, whitened postlarvae and their biofilms of an abalone farm in guangdong province. extra-cellular protease, gelatinase, lipase as well as haemolysis tests were performed on them. lysophospholipase (tlh)-targeted pcr was also carried out in order to reveal if the haemolysis caused by bacterial strains were re ... | 2005 | 16245888 |
| isolation and identification of shewanella alga and its pathogenic effects on post-larvae of abalone haliotis diversicolor supertexta. | | 2006 | 16911538 |
| characterization of a rel\nf-kappab homologue in a gastropod abalone, haliotis diversicolor supertexta. | rel\nf-kappab signal transduction pathway is evolutionarily conserved and involved in numerous biological processes. we report here, for the first time that a homologue of rel\nf-kappab transcription factor, ab-rel, was identified and functionally characterized in a gastropod abalone, h. diversicolor supertexta. the full-length ab-rel cdna consists of 1943 bp with an orf encoding a 584 amino acids protein. amino acid sequence analysis revealed that ab-rel shares conserved signature motifs with o ... | 2007 | 16949156 |
| magnetocapture of abalone transcription factor nf-kappab: a new strategy for isolation and detection of nf-kappab both in vitro and in vivo. | rel\nf-kappab plays an important role in the immune response. recently, a homologue of rel\nf-kappab transcription factor was identified from a gastropod abalone, haliotis diversicolor supertexta, named ab-rel. we present here a new strategy, so called magnetocapture, based on magnetic separation that was developed to isolate and detect this abalone transcriptional factor. with this method, ab-rel was successfully isolated from abalone haemocytes and the recombinant ab-rel protein from an insect ... | 2007 | 16978726 |
| bacterial expression and characterization of molluscan ido-like myoglobin. | the indoleamine 2,3-dioxygenase (ido)-like myoglobin (mb) is a unique type of mb isolated from the buccal mass of several archgastropod species. here, we expressed sulculus diversicolor ido-like mb as a gst-fusion protein in bacteria. the visible spectrum of gst-fusion ido-like mb shows characteristic alpha- and beta-peaks, indicating that it binds oxygen. to identify residues important in heme and oxygen binding, we constructed site-directed mutants. we initially replaced each of the 7 histidin ... | 2007 | 17276715 |
| isolation and characterization of pathogenic vibrio parahaemolyticus from diseased post-larvae of abalone haliotis diversicolor supertexta. | mass mortality among the post-larvae of cultured abalone haliotis diversicolor supertexta has occurred on the south coast of china since 2002. the diseased abalone are generally 10 to 30 days old, and typical signs of the disease include them turning white in colour and falling off the diatom films on which they were cultured. among sixteen different motile bacteria isolated from the diseased post-larvae, four were identified as vibrio parahaemolyticus on the basis of biochemical characteristics ... | 2007 | 17304623 |
| characterization and identification of virulent klebsiella oxytoca isolated from abalone (haliotis diversicolor supertexta) postlarvae with mass mortality in fujian, china. | an epidemic of mass mortality of abalone (haliotis diversicolor supertexta) postlarvae aged 40 days or less has existed across south coast of china since the second half of 2002. among 20 bacterial strains isolated from diseased abalone postlarvae on 2216e marine agar plates during an outbreak of postlarval disease in august 2005, a predominant strain (designated strain 20) was demonstrated to be virulent to postlarvae with an ld(50) value of 1.0x10(5) colony forming units (cfuml(-1)) on day 4, ... | 2008 | 17716687 |
| molecular cloning and responsive expression of macrophage expressed gene from small abalone haliotis diversicolor supertexta. | the complete cdna sequence of macrophage expressed gene (sampeg1), a perforin-like molecule, was isolated from small abalone (haliotis diversicolor supertexta) by homology cloning and rapid amplification of cdna ends (race). the full-length cdna of sampeg1 was 2781 bp, consisting of a 5'-terminal untranslated region (utr) of 252 bp, a 3'-terminal utr of 342 bp with a signal sequence taa and a poly (a) tail, and an open reading frame of 2184 bp. the deduced protein (sampeg1) was composed of 728 a ... | 2008 | 18255313 |
| cloning, characterization and tbt exposure response of cuzn superoxide dismutase from haliotis diversicolor supertexta. | the full-length cdna and genomic dna of a cytoplasmic copper, zinc superoxide dismutase (cuzn-sod) were cloned from the hepatopancreas of small abalone haliotis diversicolor supertexta by rt-pcr, race and tail pcr. the full-length cytoplasmic cuzn-sod cdna (designated sasod) comprises 984 bp. its orf encodes a polypeptide of 154 amino acids with a predicted molecular mass of 15.7 kda and theoretical isoelectric point of 6.30. the deduced amino acid (designated sasod) shares a common consensus pa ... | 2009 | 18360784 |
| identification of the up-regulated expression genes in hemocytes of variously colored abalone (haliotis diversicolor reeve, 1846) challenged with bacteria. | variously colored abalone (haliotis diversicolor reeve, 1846), which is an important commercial aquatic species and has been widely cultured, frequently suffers from bacterial infection. knowledge of the defense mechanism in this animal is still lacking and, so far few genes related to immune responses in abalones have been reported. in order to isolate differentially expressed genes in h. diversicolor challenged with bacteria, a forward suppression subtractive hybridization (ssh) cdna library w ... | 2008 | 18538840 |
| [construction of ssh library from haemocyte of variously colored abalone challenged with bacteria and differential expression analysis of macrophage expressed protein]. | abalones are considered to be the most precious delicacy from the sea, and become very important commercial seafood in aquaculture worldwide. variously colored abalone (haliotis diversicolor reeve, 1846) has been widely cultured on the southeast coast for more than twenty years. however, abalone culture frequently suffers from bacterial infection and mass mortality of reared abalones causes serious economic losses. unfortunately, knowledge of the defense mechanism in this animal is still lacking ... | 2008 | 18779156 |
| unfolding and inactivation of abalone (haliotis diversicolor) alkaline phosphatase during denaturation by guanidine hydrochloride. | abalone, a kind of low poikilothermic invertebrate, is easily exposed to ocean environment stress. since it is one of the important mariculture animals, the attention paid to the abalone study becomes increasing. alkaline phosphatase (alpase, ec 3.1.3.1) is a kind of zinc-contained metalloenzyme, which catalyzes the nonspecific hydrolysis of phosphate monoesters. unfolding and inactivation of alpase from abalone (haliotis diversicolor) during denaturation by guanidine hydrochloride (guhcl) of di ... | 2009 | 18931946 |
| toxicity of seven phthalate esters to embryonic development of the abalone haliotis diversicolor supertexta. | the toxicity of seven phthalate esters (paes), dimethyl phthalate (dmp), diethyl phthalate (dep), dibutyl phthalate (dbp), butylbenzyl phthalate (bbp), di-n-hexyl phthalate (dnhp), di-(2-ethylhexyl) phthalate (dehp) and di-n-octyl phthalate (dop) to embryogenesis and larval development of the marine univalve haliotis diversicolor supertexta was examined by means of two-stage embryo toxicity test. at the blastula stage, the normal embryonic development of h. diversicolor supertexta showed a good ... | 2009 | 19030987 |
| identification and functional characterization of a novel cytidine deaminase in a gastropod abalone, haliotis diversicolor supertexta. | cytidine deaminase (cda, also designated cdd) is a zinc-dependent enzyme involved in the pyrimidine salvage pathways and becoming very important in anticancer and antiviral therapy. here we report the identification and characterization of a cda homologue in abalone, which we named ab-cda. the analysis of the amino acids sequence revealed that the ab-cda shares conserved signature motifs and belongs to homotetrameric class of cda family. real-time pcr analysis indicated that the ab-cda was ubiqu ... | 2009 | 19150626 |
| immunomodulation in the marine gastropod haliotis diversicolor exposed to benzo(a)pyrene. | it has been reported that environmental pollutants in the aquatic ecosystem could weaken immune competence of organisms. the purpose of the present study was to investigate the effects of benzo(a)pyrene [b(a)p] on immunomodulation in marine gastropods and to see if these effects are caused by or related to the generation of reactive oxygen species. in our present study, the marine gastropod haliotis diversicolor was exposed to sublethal concentrations (0.01, 0.02, 0.04 and 0.08 mg l(-1)) of b(a) ... | 2009 | 19171366 |
| gene cloning of a sigma class glutathione s-transferase from abalone (haliotis diversicolor) and expression analysis upon bacterial challenge. | glutathione s-transferases (gsts) are a multigene family of xenobiotic metabolizing phase ii detoxification enzymes which take part in many pathological and physiological processes, and which can potentially be used as indicators and biomarkers for cancer diagnoses and organic or inorganic pollutant exposure. in this study, a full-length cdna of a sigma class gst (abgstsigma) (genbank accession number ef546619) from variously colored abalone (haliotis diversicolor) was identified. it was 1328bp ... | 2009 | 19414031 |
| molecular cloning and characterization of macrophage migration inhibitory factor from small abalone haliotis diversicolor supertexta. | the macrophage migration inhibitory factor (mif) cdna and its genome were cloned from small abalone haliotis diversicolor supertexta. small abalone mif (samif) was originally identified from an expressed sequence tag (est) fragment from a normalized cdna library. it's 5' untranslated region (utr) was obtained by 5' rapid amplification of cdna end (race) techniques and its genomic dna was cloned by pcr. the full-length cdna of samif was of 535 bp, consisting of a 5'-terminal utr of 49 bp, an open ... | 2009 | 19426810 |
| molecular cloning and expression analysis of a beta-thymosin homologue from a gastropod abalone, haliotis diversicolor supertexta. | the beta-thymosins are a family of highly conserved small peptides with multiple functions. in this study, we isolated the full-length cdna of a beta-thymosin homologue from a gastropod abalone haliotis diversicolor supertexta which we named ab-tmsb. the full-length cdna of ab-tmsb consists of 499 bp with an orf encoding a 43 amino acids protein. the deduced amino acid sequence of ab-tmsb shows 61-76% identity to other beta-thymosins and shares a conserved actin-binding domain. the phylogenetic ... | 2009 | 19524680 |
| first identification and functional analysis of a histidine triad nucleotide binding protein in an invertebrate species haliotis diversicolor supertexta. | histidine triad nucleotide binding protein (hint) represents the most ancient and widespread branches in the histidine triad superfamily. hint plays an important role in many biological processes especially in cell biology, and it has been found in a wide variety of species. however, the functional attributes of hint homologues in invertebrates have not yet been reported. here we identified a hint homologue in abalone, which we named ab-hint. the ab-hint shows significant structural and function ... | 2010 | 19720079 |
| identification and analysis of a cu/zn superoxide dismutase from haliotis diversicolor supertexta with abalone juvenile detached syndrome. | a partial cdna sequence of a putative cu/zn superoxide dismutase (sod) from haliotis diversicolor supertexta with abalone juvenile detached syndrome (ajds) was isolated by suppression subtractive hybridization library screening. the full 988 base pair (bp) abalone cu/zn sod cdna representing full cdna coding sequence was obtained by rapid amplification of cdna ends (race), and included a 462-bp open reading frame encoding 154 amino acids, plus 49 bp of 5'-, and 477 bp of 3'-untranslated region. ... | 2010 | 19951710 |
| molecular cloning and characterization of prohormone convertase 1 gene in abalone (haliotis diversicolor supertexta). | prohormone convertases (pcs) are calcium-dependent serine endoproteases of the subtilisin family that play a key role in the posttranslational processing of precursors for bioactive peptides. in this study, the cdna of pc1 from abalone (haliotis diversicolor supertexta) was cloned and sequenced. the pc1 cdna consisted of 2216 bp with an open reading frame of 2010 bp encoding a 670 amino acid peptide. comparative structural analysis revealed that abalone pc1 shared high similarity and identity wi ... | 2010 | 20044021 |
| first molecular cloning of a molluscan caspase from variously colored abalone (haliotis diversicolor) and gene expression analysis with bacterial challenge. | mammal caspases have been demonstrated to possess important functions in apoptosis and immune signaling, but there is less knowledge available on abalone caspases. in the present study, a molluscan caspase gene, abcaspase, was cloned for the first time from the variously colored abalone (haliotis diversicolor) and its full-length cdna sequence was 2427 bp, with a 1008 bp of open reading frame encoding a protein of 336 aa. the molecular mass of the deduced protein was approximately 36.97 kda with ... | 2010 | 20045058 |
| a proteomics based approach to assessing the toxicity of bisphenol a and diallyl phthalate to the abalone (haliotis diversicolor supertexta). | the contamination of marine ecosystems by endocrine disrupting compounds (edcs) is of great concern. protein expression profile maybe a good method to help us understand the molecular mechanisms of edcs-toxicity to aquatic organisms. in this study, the abalone (haliotis diversicolor supertexta), was selected as the target organism. toxicological effects of two reference endocrine disruptors: diallyl phthalate (dap, 50microgl(-1)) and bisphenol-a (bpa, 100microgl(-1)) were investigated after a th ... | 2010 | 20189630 |
| expressed sequence tag analysis for identification and characterization of genes related to tributyltin (tbt) exposure in the abalone haliotis diversicolor supertexta. | the analysis of expressed sequence tags (ests) is an efficient approach for gene discovery, expression profiling, and the development of resources for functional genomics. to analyze the transcriptome response to tributyltin of small abalone haliotis diversicolor supertexta, a normalized cdna library of hepatopancreas after exposure of animals to tributyltin was constructed. three thousand and forty eight high quality ests were generated. after processing, a total of 2473 unigenes comprising 370 ... | 2009 | 20403754 |
| a modified enrichment method to construct microsatellite library from plateau pika genome (ochotona curzoniae). | a microsatellite-enriched library of plateau pika (ochotona curzoniae) was constructed according to the strong affinity between biotin and streptavidin. firstly, genomic dna was fragmented by ultrasonication, which is a major improvement over traditional methods. linker-ligated dna fragments were hybridized with biotinylated microsatellite probes, and then were subjected to streptavidin-coated magnetic beads. pcr amplification was performed to obtain double-stranded dna fragments containing micr ... | 2010 | 20451163 |
| innate immune parameters and haemolymph protein expression profile to evaluate the immunotoxicity of tributyltin on abalone (haliotis diversicolor supertexta). | the immunotoxicity of tributyltin (tbt) on marine gastropods has been comparatively little studied although risks to wildlife associated with this compound are well known. in this study, a 30-day trial was conducted to evaluate the immunotoxic effects on abalone (haliotis diversicolor supertexta) by exposing a range of doses of tbt (0, 2, 10, and 50 ng/l). innate immune parameters, including phagocytic ability (pa), lysozyme activity, phenoloxidase (po) level and superoxide dismutase (sod) activ ... | 2010 | 20488199 |
| responses of abalone haliotis diversicolor to sublethal exposure of waterborne and dietary silver and cadmium. | in this study, we examined the chronic waterborne and dietary exposure of silver (ag) and cadmium (cd) to the abalone haliotis diversicolor using various endpoints such as growth and feeding rates, metal body burden, subcellular distribution, and metallothionein (mt) concentration over a period of 7 weeks of exposure. the growth and feeding rates of abalones were inhibited during the early stage of exposure to different extents, but then recovered to nearly the control levels. a large portion of ... | 2010 | 20570350 |
| withering syndrome in the abalone haliotis diversicolor supertexta. | abalone aquaculture is a small but growing industry in thailand and is based on both the exotic haliotis diversicolor supertexta and the native h. asinina. withering syndrome (ws) in abalone is caused by an infection with the rickettsia-like organism (rlo) 'candidatus xenohaliotis californiensis' and has been spread to many countries globally. the present study reports the first observation of the ws-rlo agent in the small abalone, h. diversicolor supertexta in thailand, taiwan (roc) and the peo ... | 2010 | 20597432 |
| tributyltin toxicity in abalone (haliotis diversicolor supertexta) assessed by antioxidant enzyme activity, metabolic response, and histopathology. | a toxicity test was performed to investigate the possible harmful effects of tributyltin (tbt) on abalone (haliotis diversicolor supertexta). animals were exposed to tbt in a range of environmentally relevant concentrations (2, 10 and 50 ng/l) for 30 days under laboratory conditions. tbt-free conditions were used as control treatments. the activity of antioxidant enzymes superoxide dismutase (sod) and peroxidase (pod), and malondialdehyde (mda), along with levels of haemolymph metabolites, and h ... | 2010 | 20709453 |
| identification and functional characterization of an rbx1 in an invertebrate haliotis diversicolor supertexta. | rbx1 (ring box1) is an evolutionarily conserved ring-h2 finger protein and belongs to the ring-finger family of ubiquitin ligase e3, which determines the substrate specificity of ubiquitination and regulates a variety of biological processes. we report here the identification and functional characterization of an rbx1 homologue in abalone, which we named ab-rbx1. ab-rbx1 contains conserved cysteine/histidine residues which are the characteristics of rbx proteins. phylogenetic tree analysis furth ... | 2011 | 20801156 |
| the impacts of bisphenol a (bpa) on abalone (haliotis diversicolor supertexta) embryonic development. | the effects of bisphenol a (bpa) on abalone (haliotis diversicolor supertexta) embryonic development were investigated by exposing the fertilized eggs to four different concentrations of bpa (0.05, 0.2, 2 and 10 μg ml(-1)). toxicity endpoints including the embryo development parameters, the physiological features and the expression profile of several reference genes (prohormone convertase 1, pc1; cyclin b, cb; and cyclin-dependent kinase 1, cdk1) were assessed. the results showed that bpa could ... | 2010 | 20970156 |
| identification and expression localization of a ran homologue in mollusc abalone, haliotis diversicolor supertexta. | ran protein is a central molecular in several key nuclear functions, including nucleocytoplasmic transport, cell-cycle progression and nuclear envelope assembly. in this study, we have isolated and characterized a ran homologue from a gastropod abalone which we named ab-ran. the full-length cdna consists of 1239 bp with an orf encoding a 220 amino acid protein. the deduced amino acid sequence of ab-ran shows highly similar to that of other ran members (84-88%). moreover, the ab-ran contains five ... | 2011 | 21232607 |
| passive immune-protection of small abalone against vibrio alginolyticus infection by anti-vibrio igy-encapsulated feed. | small abalone (haliotis diversicolor supertexta) is a high value-added shellfish. it however has been suffering vibrio alginolyticus infections, which cause mass death of small abalone and thus great economic losses, particularly in artificial aquaculture. in this study, we attempted to treat small abalone with anti-vibrio igy to elicit a passive immunity directly against v. alginolyticus infections. anti-vibrio igy was alginate encapsulated in egg powders as feed, which may avoid antibody inact ... | 2011 | 21300158 |
| molecular cloning and expression of interleukin-1 receptor-associated kinase 4, an important mediator of toll-like receptor signal pathway, from small abalone haliotis diversicolor. | mammal interleukin-1 receptor-associated kinases (iraks) have been demonstrated to play important functions in tlrs (toll-like receptor) signal pathway and t cell proliferation, but there is less knowledge available on mollusc iraks. in this study, a molluscan irak-4 gene, sairak-4, was cloned for the first time from the small abalone (haliotis diversicolor). its full-length cdna sequence was 2062 bp, with a 1548 bp open reading frame encoding a protein of 516 aa. the molecular mass of the deduc ... | 2011 | 21362486 |
| potential mechanisms of phthalate ester embryotoxicity in the abalone haliotis diversicolor supertexta. | the effects and associated toxicological mechanisms of five phthalate esters (paes) on abalone embryonic development were investigated by exposing the embryos to a range of paes concentrations (0.05, 0.2, 2 and 10 µg/ml). the results showed that paes could significantly reduce embryo hatchability, increase developmental malformations, and suppress the metamorphosis of abalone larvae. the possible toxicological mechanisms of paes to abalone embryos included, affecting the na+-k+-pump and ca2+-mg2 ... | 2011 | 21377254 |
| tio2 nanoparticles in the marine environment: impact on the toxicity of tributyltin to abalone (haliotis diversicolor supertexta) embryos. | little information is available on the potential ecotoxicity of manufactured nanomaterials (mnms) in the marine environment. to carefully address this issue, the toxicity of nanosized titanium dioxide (ntio(2)) aggregates in the marine environment was evaluated using abalone (haliotis diversicolor supertexta) embryonic development as a model. the effect of ntio(2) aggregates on the toxicity of the highly toxic marine antifouling compound tributyltin (tbt) to abalone embryos was also investigated ... | 2011 | 21413738 |
| assessing abalone growth inhibition risk to cadmium and silver by linking toxicokinetics/toxicodynamics and subcellular partitioning. | the purpose of this study was to link toxicokinetics/toxicodynamics and subcellular partitioning for assessing the susceptibility and the growth inhibition risks of abalone haliotis diversicolor supertexta exposed to waterborne and foodborne cadmium (cd) and silver (ag). we reanalyzed published data on growth inhibition and subcellular partitioning associated with the present mechanistic model to explore the correlations among elimination (k (e)), detoxification (k (d)), and recovery (k (r)) rat ... | 2011 | 21437626 |
| acute toxicity of nonylphenols and bisphenol a to the embryonic development of the abalone haliotis diversicolor supertexta. | acute toxic effects and mechanisms of two typical endocrine disrupting chemicals, nonylphenols (nps) and bisphenol a (bpa), to the embryonic development of the abalone haliotis diversicolor supertexta, were investigated by the two-stage embryo toxicity test. the 12-h median effective concentrations (ec(50)) of nps and bpa to the trochophore development were 1016.22 and 30.72 μg l(-1), respectively, and the respective 96-h ec(50) values based on the completion of metamorphosis (another experiment ... | 2011 | 21479784 |
| identification and functional characterization of a putative 17β-hydroxysteroid dehydrogenase 12 in abalone (haliotis diversicolor supertexta). | the 17β-hydroxysteroid dehydrogenases (17β-hsds) are key enzymes in the downstream process of steroid hormone biosynthesis. to date, relatively little is known about the role of 17β-hsds in marine gastropods. in the present study, a putative cdna sequence encoding type 12 17β-hsd (17β-hsd-12) was identified in abalone (haliotis diversicolor supertexta). the full-length cdna was 1,978 bp, including an open reading frame (orf) of 963 bp that encoded a protein of 321 amino acids. comparative struct ... | 2011 | 21479786 |
| the toxicity and oxidative stress of tio(2) nanoparticles in marine abalone (haliotis diversicolor supertexta). | the increasing use of nanotechnology highlights the need to understand and clarify the environmental impacts of nanomaterials. in this study, the acute toxicity and oxidative stress of titanium dioxide nanoparticles (ntio(2)) in mature marine abalone (haliotis diversicolor supertexta) were assessed. no acute effect was found in any of the treatment groups with ntio(2) concentration gradients ranging from 0.1 to 10mg/l. however, the activity of an antioxidant enzyme superoxide dismutase (sod) sig ... | 2011 | 21492882 |
| effects of tributyltin and benzo[a]pyrene on the immune-associated activities of hemocytes and recovery responses in the gastropod abalone, haliotis diversicolor. | our previous study reports that short-term exposure to sublethal concentrations of benzo[a]pyrene (bap) induces immunomodulation in the gastropod abalone, haliotis diversicolor. in the present study, it was further observed that long-term chronic exposure to sublethal concentrations of bap modulated the immunocompetence of abalones in terms of the change in activity of the antioxidant and immune associated parameters tested. in addition, the effect of tributyltin (tbt), another important genotox ... | 2011 | 21549218 |
| development and characterization of 60 microsatellite markers in the abalone haliotis diversicolor. | the abalone, haliotis diversicolor, is one of the most important mariculture species in southern china. we developed 60 new polymorphic microsatellite markers for h. diversicolor and characterized them in 30 individuals from a cultured population in sanya, china. all 60 markers were found to be polymorphic. the number of alleles ranged from two to nine per locus, with an average of 4.12/locus. the expected and observed heterozygosities ranged from 0.10 to 0.88 and from 0.07 to 0.87, respectively ... | 2011 | 21574142 |
| construction of an integrated map of haliotis diversicolor using microsatellite markers. | small abalone, haliotis diversicolor, is naturally distributed along the coastal waters of east asia from japan to the philippines. it is an economically important maricultured species in southern china and taiwan. genetic linkage maps for small abalone were constructed using a total of 308 simple sequence repeat markers including 297 novel markers. segregation data on 96 progeny were genotyped using a pseudo-testcross strategy. sixteen linkage groups were identified in both female and male maps ... | 2011 | 21617897 |
| development of 11 polymorphic microsatellite loci in the small abalone (haliotis diversicolor reeve). | eleven polymorphic microsatellite loci for haliotis diversicolor were isolated and characterized from (ct)(n) - and (ac)(n) -enriched library. they were tested in 24 individuals from a natural population. all of them were polymorphic, with the number of alleles varying between three and 10. the observed heterozygosities and expected heterozygosities ranged from 0.083 to 0.913 and from 0.159 to 0.856, respectively. the 11 isolated microsatellite loci, except sa-jmu6 and sa-jmu12, followed hardy-w ... | 2008 | 21586054 |
| gene expression profiling in respond to tbt exposure in small abalone haliotis diversicolor. | in this study, we investigated the gene expression profiling of small abalone, haliotis diversicolor by tributyltin (tbt) exposure using a cdna microarray containing 2473 unique transcripts. totally, 107 up-regulated genes and 41 down-regulated genes were found. for further investigation of candidate genes from microarray data and est analysis, quantitative real-time pcr was performed at 6 h, 24 h, 48 h, 96 h and 192 h tbt exposure. 26 genes were found to be significantly differentially expresse ... | 2011 | 21767652 |
| thermodynamic characterization of muscle tropomyosins from marine invertebrates. | structural properties of invertebrate muscle tropomyosin (tm) have not been characterized in detail to date. tms were thus purified from the mantle muscle of japanese common squid todarodes pacificus, the foot muscle of tokobushi abalone haliotis diversicolor and the tail muscle of kuruma prawn marsupenaeus japonicus, and investigated for their thermodynamic properties by circular dichroism (cd) spectrometry and differential scanning calorimetry (dsc). from the cd spectrometry data, the apparent ... | 2011 | 21722747 |
| influences of dmp on the fertilization process and subsequent embryogenesis of abalone (haliotis diversicolor supertexta) by gametes exposure. | di-methyl phthalate (dmp), a typical endocrine disrupting chemical (edc), is ubiquitously distributed in aquatic environments; yet studies regarding its impact on gametes and the resulting effects on embryogenesis in marine gastropods are relatively scarce. in this study, the influences of dmp on the gametes and subsequent developmental process of abalone (haliotis diversicolor supertexta, a representative marine benthic gastropod) were assessed. newborn abalone eggs and sperm were exposed separ ... | 2011 | 22028799 |
| mitogenome of the small abalone haliotis diversicolor reeve and phylogenetic analysis within gastropoda. | the complete mitochondrial coding regions of three small abalones haliotis diversicolor reeve, two collected from vietnam and one from southern china, were successfully sequenced. the molecular feature of the mitochondrial genome is identical with the general description of the family haliotidae mtdnas that have been sequenced so far. the sequenced nucleotides are 16,186-16,266bp in length. the mitogenome encodes 13 protein-coding genes, 2 ribosomal rna genes, and 22 transfer rna genes. between ... | 2011 | 22118637 |
| functional annotation of an expressed sequence tag library from haliotis diversicolor and analysis of its plant-like sequences. | the small abalone, haliotis diversicolor, is a widely distributed and cultured species in the subtropical coastal area of china. to identify and classify functional genes of this important species, a normalized expressed sequence tag (est) library, including 7069 high quality ests from the total body of h. diversicolor, was analyzed. a total of 4781 unigenes were assembled and 2991 novel abalone genes were identified. the gc content, codon and amino acid usage of the transcriptome were analyzed. ... | 2011 | 21867971 |
| cloning, characterization, and expression analysis of a putative 17 beta-hydroxysteroid dehydrogenase 11 in the abalone, haliotis diversicolor supertexta. | the 17-beta-hydroxysteroid dehydrogenases (17β-hsds) are key enzymes for sex steroid biosynthesis. to date, relatively little is known about the presence and function of 17β-hsds in marine gastropods. in the present study, a cdna sequence encoding putative 17β-hsd type 11 (17β-hsd-11) was identified in marine abalone (haliotis diversicolor supertexta). the full-length cdna contains 1058bp, including an open reading frame (orf) of 900bp that encodes a protein of 299 amino acids. comparative struc ... | 2012 | 22249002 |
| involvement of antizyme characterized from the small abalone haliotis diversicolor in gonadal development. | the small abalone haliotis diversicolor is an economically important mollusk that is widely cultivated in southern china. gonad precocity may affect the aquaculture of small abalone. polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. ornithine decarboxylase (odc) and antizyme (az) are essential elements of a feedback circuit that regulates cellular polyamines. this paper presents the molecular cloning and characterization of az fr ... | 2015 | 26313647 |
| pyrosequencing of haliotis diversicolor transcriptomes: insights into early developmental molluscan gene expression. | the abalone haliotis diversicolor is a good model for study of the settlement and metamorphosis, which are widespread marine ecological phenomena. however, information on the global gene backgrounds and gene expression profiles for the early development of abalones is lacking. | 2012 | 23236463 |
| the development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus dna. | a loop-mediated isothermal amplification (lamp) assay was developed for the detection of abalone herpesvirus dna. two pairs of primers were designed, based on the sequence of the dna polymerase gene of abalone herpesvirus. the reaction temperature and time were optimized to 63°c and 60min, respectively. lamp amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. the method developed was specific for the detection of abalon ... | 2014 | 24291740 |
| cloning, characterization and functional expression of taenia solium 17 beta-hydroxysteroid dehydrogenase. | the 17β-hydroxysteroid dehydrogenases (17β-hsd) are key enzymes involved in the formation (reduction) and inactivation (oxidation) of sex steroids. several types have been found in vertebrates including fish, as well as in invertebrates like caenorhabditis elegans, ciona intestinalis and haliotis diversicolor supertexta. to date limited information is available about this enzyme in parasites. we showed previously that taenia solium cysticerci are able to synthesize sex steroid hormones in vitro ... | 2014 | 24698785 |
| development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the dna polymerase gene. | a 5781-base pair (bp) fragment of genomic dna from the taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the dna polymerase gene of the abalone herpesvirus strain victoria/aus/2007. homology of the amino acid sequence with the dna polymerase of ostreid herpesvirus 1 was 30% (563/1856). in this study, a pcr-based procedure for detecting herpesvirus infection of abalone, haliotis diversicolo ... | 2012 | 22579937 |
| beta-1,3-1,6-glucan modulate the non-specific immune response to enhance the survival in the vibrio alginolyticus infection of taiwan abalone (haliotis diversicolor supertexta). | this research aims to investigate the non-specific immune response of taiwan abalone (haliotis diversicolor supertexta) which was treated with the beta-1,3-1,6-glucan to be observed in the survival impact after the vibrio alginolyticus infection. the non-specific immune and physiological response of superoxide anion radical (o2(-)), phenoloxidase (po), phagocytic index (pi), phagocytic rate (pr) and lucigenin-chemiluminescence for reactive oxygen intermediates (rois) were enhanced via in-vitro e ... | 2016 | 27150049 |
| identification and involvement of ferritin in the response to pathogen challenge in the abalone, haliotis diversicolor. | accumulating data has demonstrated that ferritin plays an important role in host defense responses against infection by pathogens in many organisms. in this study, ultracentrifugation was used to isolate ferritin from abalone, haliotis diversicolor, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis revealed that this ferritin consisted of two subunits (designated as hdfer1 and hdfer2). there are no disulfide bonds between the hdfer1 and hdfer2 subunits; however, t ... | 2016 | 26875633 |
| identification and characterization of vibrio harveyi associated with diseased abalone haliotis diversicolor. | mass mortality of farmed small abalone haliotis diversicolor occurred in fujian, china, from 2009 to 2011. among isolates obtained from moribund abalones, the dominant species ap37 exhibited the strongest virulence. after immersion challenge with 106 cfu ml-1 of ap37, abalone mortalities of 0, 53 and 67% were induced at water temperatures of 20°c, 24°c, and 28°c, respectively. following intramuscular injection, ap37 showed a low ld50 (median lethal concentration) value of 2.9 × 102 cfu g-1 (colo ... | 2013 | 23548363 |
| metabolic responses of haliotis diversicolor to vibrio parahaemolyticus infection. | vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone haliotis diversicolor. elucidation of metabolic mechanisms of abalones in responding to v. parahemolyticus infection is essential for controlling the epidemic. in this work, (1)h nmr-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of h. diver ... | 2017 | 27890800 |
| pi3k-akt signaling pathway is involved in hypoxia/thermal-induced immunosuppression of small abalone haliotis diversicolor. | the pi3k-akt signal pathway has been found to be involved in many important physiological and pathological processes of the innate immune system of vertebrates and invertebrates. in this study, the akt (hdakt) and pi3k (hdpi3k) gene of small abalone haliotis diversicolor were cloned and characterized for the important status of pi3k and akt protein in pi3k-akt signaling pathway. the full length cdnas of hdakt and hdpi3k are 2126 bp and 6052 bp respectively, encoding proteins of 479 amino acids a ... | 2016 | 27825946 |
| identification and expression analysis of immune-related genes linked to rel/nf-κb signaling pathway under stresses and bacterial challenge from the small abalone haliotis diversicolor. | inhibitor of nf-κb (iκb), nuclear factor-κb (nf-κb), and akirin2 are all important members of rel/nf-κb signaling pathway, which plays a pivotal role in regulating the innate immune response of vertebrates and invertebrates. in this study, the iκb (saiκb) and akirin2 (saakirin2) cdnas of small abalone haliotis diversicolor were cloned and characterized. the full length cdna of saiκb and saakirin2 were 1748 bp and 1452 bp respectively, encoding a protein of 401 aa and 187 aa respectively. a conse ... | 2014 | 25158315 |
| effects of the dietary administration of sodium alginate on the immune responses and disease resistance of taiwan abalone, haliotis diversicolor supertexta. | sodium alginate extracted from brown algae was reported to enhance the immune response and resistance of fish and shrimp. in this study, survival rates of the abalone, haliotis diversicolor supertexta, against vibrio parahaemolyticus, and its non-specific immune parameters such as the total haemocyte count (thc), phenoloxidase (po) activity, respiratory bursts, superoxide dismutase (sod) activity, phagocytic activity, and clearance efficiency to v. parahaemolyticus by h. diversicolor supertexta ... | 2013 | 23296117 |
| insulin-like growth factor binding protein 7, a member of insulin-like growth factor signal pathway, involved in immune response of small abalone haliotis diversicolor. | insulin-like growth factor binding protein 7 (igfbp7), the only member of the igfbp superfamily that binds strongly to insulin, may have different functions from other igfbps. unlike other igfbps, there is no knowledge available on aquatic invertebrate igfbp7. in this study, a molluscan igfbp7 gene, saigfbp7, was cloned for the first time from the small abalone haliotis diversicolor. its full-length cdna sequence is 1812 bp, with a 720 bp open reading frame encoding a protein of 239 aa. the mole ... | 2012 | 22584203 |
| viruses infecting marine molluscs. | although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. the lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. our current knowledge is mostly restricted to viruses affecting farmed species such as oysters crassostrea gigas, abalone haliotis diversicolor supertexta or the scallop chlamys farreri. molecular app ... | 2017 | 28189502 |
| evolution of molluscan hemocyanin structures. | hemocyanin transports oxygen in the hemolymph of many molluscs and arthropods and is therefore a central physiological factor in these animals. molluscan hemocyanin molecules are oligomers composed of many protein subunits that in turn encompass subsets of distinct functional units. the structure and evolution of molluscan hemocyanin have been studied for decades, but it required the recent progress in dna sequencing, x-ray crystallography and 3d electron microscopy to produce a detailed view of ... | 2013 | 23454609 |
| pseudotenacibaculum haliotis gen. nov., sp. nov., a new member within the tenacibaculum-polaribacter clade of the family flavobacteriaceae, isolated from the intestine of adult abalones, haliotis diversicolor and h. discushannai. | two rod-shaped marine bacterial strains named fdzsb0410t and fdzwpb0420, were isolated from the intestine of two adult abalone species, haliotis diversicolor and haliotis discushannai, respectively. the strains were non-flagellated, motile by gliding, oxidase-positive but catalase-negative, and agar-degrading. the two strains had identical 16s rrna gene sequences with the highest sequence similarity less than 96.0 % with species of the genera tenacibaculumand polaribacter within the family flavo ... | 2016 | 27373534 |
| draft genome sequence of the agar-degrading bacterium catenovulum sp. strain ds-2, isolated from intestines of haliotis diversicolor. | catenovulum sp. strain ds-2, isolated from intestines of haliotis diversicolor, is able to degrade agar and produce agaro-oligosaccharides. here, we report the draft genome sequence of catenovulum sp. strain ds-2. | 2014 | 24604650 |