| calcium sensitivity of abalone, haliotis discus, myosin. | superprecipitation was observed with abalone myosin and purified rabbit actin in the presence of calcium ions, but was not observed in the absence of calcium. the mg-atpase [ec 3.6.1.3] activity of abalone myosin and rabbit actin in the absence of calcium ions (egta present) showed about 60% inhibition as compared with values in the presence of calcium ions. the calcium sensitivity may be attributable to abalone myosin, as in the case of scallop myosin. | 1976 | 134995 |
| the neurosecretory staining in the cerebral ganglia of the japanese abalone (ezoawabi), haliotis discus hannai, and its relationship to reproduction. | neurosecretory staining in the cerebral ganglia of the japanese abalone (ezoawabi), haliotis discus hannai, was studied during the reproductive cycle. the variation in stain affinity of the cells and the amount of neurosecretory material contained in the cells were measured during the study. four cell types were found in the cerebral ganglion. neurosecretory activity was found in cell types a and b, and the amount of neurosecretory material in the cells and the stain intensity of cellular materi ... | 1994 | 7515014 |
| vibrio halioticoli sp. nov., a non-motile alginolytic marine bacterium isolated from the gut of the abalone haliotis discus hannai. | six alginolytic, facultatively anaerobic, non-motile marine bacteria were isolated from the gut of abalone haliotis discus hannai. dna-dna hybridization data showed that the six strains constituted a single genospecies. phylogenetic analyses of 16s rdna sequences indicated that the isolates should be assigned to the genus vibrio. the phenotypic features of the isolates were closely related to vibrio fischeri and vibrio pelagius biovar i, but 13 traits (motility, luminescence, alginase production ... | 1998 | 9731299 |
| cloning and sequence analysis of vibrio halioticoli genes encoding three types of polyguluronate lyase. | the alginate lyase-coding genes of vibrio halioticoli iam 14596(t), which was isolated from the gut of the abalone haliotis discus hannai, were cloned using plasmid vector puc 18, and expressed in escherichia coli. three alginate lyase-positive clones, pvhb, pvhc, and pvhe, were obtained, and all clones expressed the enzyme activity specific for polyguluronate. three genes, alyvg1, alyvg2, and alyvg3, encoding polyguluronate lyase were sequenced: alyvg1 from pvhb was composed of a 1056-bp open r ... | 2000 | 10804241 |
| abalone collagens: immunological properties and seasonal changes of their mrna levels. | the antisera were raised against pepsin-solubilized abalone collagen and its corresponding gelatin. the reactivity against abalone collagen was higher with the anti-collagen than anti-gelatin antiserum. the two antisera recognized all type i collagens from various vertebrates, whereas these had no reactivity against vertebrate type iii and type v collagens. furthermore, both antisera reacted with only alpha 2(i) chains from chicken, rat, and calf. the strong reactivity was observed against the t ... | 2000 | 10825665 |
| dictyterpenoids a and b, two novel diterpenoids with feeding-deterrent activity from the brown alga dilophus okamurae. | in our continuing efforts to study the presence of biologically active compounds that control seaweed-herbivore interactions, we examined the presence of such chemicals in freshly collected dilophus okamurae dawson. our results demonstrated the presence of two diterpenoids, named dictyterpenoid a (1) and dictyterpenoid b (2), having a novel carbon skeleton, along with 10 known diterpenes, 3-12. the structures of these novel metabolites were elucidated by spectroscopic and chemical methods. the i ... | 2002 | 11858741 |
| purification and cdna cloning of a cellulase from abalone haliotis discus hannai. | a cellulase [endo-beta-1,4-d-glucanase (ec 3.2.1.4)] was isolated from the hepatopancreas of abalone haliotis discus hannai by successive chromatographies on toyopearl cm-650m, hydroxyapatite and sephacryl s-200 hr. the molecular mass of the cellulase was estimated to be 66 000 da by sds/page, thus the enzyme was named hdeg66. the hydrolytic activity of hdeg66 toward carboxymethylcellulose showed optimal temperature and ph at 38 degrees c and 6.3, respectively. cdnas encoding hdeg66 were amplifi ... | 2003 | 12581217 |
| cdna cloning of an alginate lyase from abalone, haliotis discus hannai. | an alginate lyase, termed hdaly in the present paper, was isolated from the hepatopancreas of abalone, haliotis discus hannai, by ammonium sulfate fractionation, followed by toyopearl cm-650m column chromatography. enzymatic properties of hdaly were similar to those of previously reported haliotis and turbo poly(m) lyases, e.g., it preferentially degraded a poly(beta-d-mannuronate)-rich substrate with an optimal ph and temperature at ph 8.0 and 45 degrees c, respectively. in order to determine t ... | 2003 | 14667705 |
| inheritance of microsatellite dna markers in the pacific abalone haliotis discus hannai. | microsatellite markers have been developed for a variety of abalones, and locus-specific homozygote excesses at population level have been recorded for microsatellite loci. to ascertain whether null alleles exist at microsatellite loci in the pacific abalone, we studied the mode of inheritance of 7 microsatellite loci in 4 families with a reciprocal cross of 2 females x 2 males. all loci segregated codominantly, but only 3 loci ( hdh1321, hdh78, and hdd108c) conformed to mendelian segregation an ... | 2003 | 14723229 |
| vibrio neonatus sp. nov. and vibrio ezurae sp. nov. isolated from the gut of japanese abalones. | five alginolytic, facultative anaerobic, non-motile bacteria were isolated from the gut of japanese abalones (haliotis discus discus, h. diversicolor diversicolor and h. diversicolor aquatilis). phylogenetic analyses based on 16s rrna gene and gap gene sequences indicated that these strains are closely related to v. halioticoli. dna-dna hybridizations, faflp fingerprintings, and phylogenies of gap and 16s rrna gene sequences showed that the five strains represent two species different from all c ... | 2004 | 15490553 |
| complementary dna cloning and molecular evolution of opine dehydrogenases in some marine invertebrates. | the complete complementary dna sequences of genes presumably coding for opine dehydrogenases from arabella iricolor (sandworm), haliotis discus hannai (abalone), and patinopecten yessoensis (scallop) were determined, and partial cdna sequences were derived for meretrix lusoria (japanese hard clam) and spisula sachalinensis (sakhalin surf clam). the primers odh-9f and odh-11r proved useful for amplifying the sequences for opine dehydrogenases from the 4 mollusk species investigated in this study. ... | 2004 | 15565272 |
| preparation and characterization of monoclonal antibody against abalone allergen tropomyosin. | muscle protein tropomyosin is a major and common allergen of mollusks. in order to develop immunoassays based on monoclonal antibody (mab) for allergen characterization, a mab against japanese abalone (haliotis discus) was prepared and characterized in the present study. in comparison with the ige reactivities of sera from crustacean allergic individuals, the selected mab ae9f9 showed specific reaction to the abalone allergenic tropomyosin. the mab ae9f9 reacted to the crustaceans including lobs ... | 2004 | 15684662 |
| purification and characterization of a novel extracellular beta-1,3-glucanase produced by bacillus clausii nm-1 isolated from ezo abalone haliotis discus hannai. | a novel extracellular alkaline stable beta-1,3-glucanase produced by bacillus clausii nm-1 isolated from the ezo abalone haliotis discus hannai was purified by ammonium sulfate precipitation, deae-sepharose ff ion exchange chromatography and sephacryl s-200hr gel filtration. the molecular weight of the purified enzyme was estimated to be 71 kda from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the enzyme was very stable at ph 5.3 to 11.5 but unstable at ph 4.0 to 4.5. th ... | 2003 | 16233365 |
| segregation and linkage analysis of 75 novel microsatellite dna markers in pair crosses of japanese abalone (haliotis discus hannai) using the 5'-tailed primer method. | we present novel microsatellite markers of the japanese abalone (haliotis discus hannai) for general mapping studies in this species. a total of 75 microsatellite markers were developed, and the allele-transmission patterns of these markers were studied in three families generated by pair crosses. for allele scoring, we employed the 5'-tailed primer polymerase chain reaction (pcr) technique, which substantially reduces the cost for fluorescent labeling of primers. of the 225 possible marker-fami ... | 2006 | 16874445 |
| cloning and expression analysis of a hsp70 gene from pacific abalone (haliotis discus hannai). | heat shock protein 70 (hsp70), the primary member of hsps that are responsive of thermal stress, is found in all multicellular organisms and functions mostly as molecular chaperon. the inducible hsp70 cdna cloned from pacific abalone (haliotis discus hannai) using rapid amplification of cdna ends (race), was highly homologous to other hsp70 genes. the full-length cdna of the pacific abalone hsp70 was 2631bp, consisting of a 5'-terminal untranslated region (utr) of 90bp, a 3'-terminal utr of 573b ... | 2007 | 16901719 |
| molecular cloning and characterization of mn-superoxide dismutase from disk abalone (haliotis discus discus). | the mitochondrial enzyme manganese superoxide dismutase (mitmn-sod) is one of the antioxidant enzymes involved in cellular defense against oxidative stress and catalyzes the conversion of o(2)(-) into the stabler h(2)o(2). in this study, a putative gene encoding mn-sod from disk abalone (haliotis discus discus, amn-sod) was cloned, sequenced, expressed in escherichia coli k12(tb1) and the protein was purified using pmal protein purification system. sequencing resulted orf of 681 bp, which corres ... | 2006 | 17020816 |
| first report of invertebrate mx: cloning, characterization and expression analysis of mx cdna in disk abalone (haliotis discus discus). | myxovirus resistance (mx) protein is one of the most studied antiviral proteins. it is induced by the type i interferon system (ifn alpha/beta) in various vertebrates, but its expression has not been identified or characterized in mollusks or other multi-cellular invertebrates to date. in this study, we isolated the mx gene from a disk abalone (haliotis discus discus) normalized cdna library. mx cdna was sequenced, cloned and compared to other known mx proteins. the full-length 1664 bp of abalon ... | 2007 | 17097889 |
| genetic differences between hatchery stocks and natural populations in pacific abalone (haliotis discus) estimated using microsatellite dna markers. | genetic variations within and between nine hatchery stocks and seven natural populations of abalone including ezo-abalone (haliotis discus hannai) and kuro-abalone (h. d. discus) were assayed with nine microsatellite markers. marked reductions of genetic variability in the hatchery stocks were recognized in the allelic diversity and mean heterozygosity compared with the natural populations. thirteen of 16 significant hwe deviations in hatchery stocks revealed heterozygotes excess, while all natu ... | 2007 | 17294314 |
| two ferritin subunits from disk abalone (haliotis discus discus): cloning, characterization and expression analysis. | ferritin plays a key role in cellular iron metabolism, which includes iron storage and detoxification. from disk abalone, haliotis discus discus, the cdna that encodes the two ferritin subunits abalone ferritin subunit 1 (abf1) and abalone ferritin subunit 2 (abf2) were cloned. the complete cdna coding sequences for abf1 and abf2 contained 621 and 549 bp, encoding for 207 and 183 amino acid residues, respectively. the h. discus discus abf2 subunit contained a highly conserved motif for the ferro ... | 2007 | 17442591 |
| vibrio comitans sp. nov., vibrio rarus sp. nov. and vibrio inusitatus sp. nov., from the gut of the abalones haliotis discus discus, h. gigantea, h. madaka and h. rufescens. | nine alginolytic, facultatively anaerobic, non-motile bacteria were isolated from the guts of the abalones haliotis discus discus, h. gigantea, h. madaka and h. rufescens. phylogenetic analyses based on 16s rrna gene sequences indicated that these bacteria were closely related to vibrio superstes g3-29(t) (98.6-99.3 % sequence similarity). dna-dna hybridization and phylogenetic analysis based on the gapa gene demonstrated that six strains constituted one bacterial species, two strains represente ... | 2007 | 17473233 |
| molecular cloning and expression analysis of interferon-gamma inducible lysosomal thiol reductase (gilt)-like cdna from disk abalone (haliotis discus discus). | interferon gamma (ifn-gamma) inducible lysosomal thiol reductase (gilt) has been described as a key enzyme in processing and presentation of major histocompatibility complex (mhc) class ii restricted antigen (ag) by catalyzing disulfide bond (s-s) reduction in mammals. abalone gilt-like (abgilt) full-length cdna was isolated from the normalized disk abalone cdna library. the 807-bp abgilt cdna consists of an open reading frame of 684-bp, encoding 228 amino acid residues. the predicted abgilt pro ... | 2007 | 17599346 |
| shewanella haliotis sp. nov., isolated from the gut microflora of abalone, haliotis discus hannai. | a motile, rod-shaped, pink-orange pigmented bacterium, designated strain dw01(t), was isolated from the gut microflora of abalone collected from the south sea (republic of korea). cells were gram-negative, facultatively anaerobic, catalase- and oxidase-positive. the major fatty acids were iso-c(15 : 0) (17.7 %), c(16 : 0) (13.4 %), iso-c(15 : 0) 2-oh and/or c(16 : 1)omega7c (12.5 %) and c(17 : 1)omega8c (10.7 %). the dna g+c content was 53.7 mol%. a phylogenetic tree based on the 16s rrna gene s ... | 2007 | 18048751 |
| comparative study of two thioredoxin peroxidases from disk abalone (haliotis discus discus): cloning, recombinant protein purification, characterization of antioxidant activities and expression analysis. | thioredoxin peroxidase (tpx), also named peroxiredoxin (prx), is an important peroxidase, which can protect organisms against various oxidative stresses. two tpxs were isolated from a disk abalone (haliotis discus discus) cdna library, named as abtpx1 and abtpx2, respectively. abtpx1 and abtpx2 consist of 1315 and 1045 bp full-length cdna with 753 and 597 bp open reading frames encoding 251 and 199 amino acids, respectively. the tpx signature motif 1 (fypldftfvcptei) and motif 2 (gevcpa) were co ... | 2008 | 18226547 |
| mitochondrial thioredoxin-2 from disk abalone (haliotis discus discus): molecular characterization, tissue expression and dna protection activity of its recombinant protein. | thioredoxin-2 is a mitochondria-specific member of the thioredoxin (trx) super-family that plays an important role as a component of the mitochondrial antioxidant system. the gene coding mitochondrial trx-2 was isolated from the disk abalone (haliotis discus discus) cdna library, denoted as abtrx-2. it contains 1214-bp full length with 519-bp open reading frame, encoding 173 amino acids. abtrx-2 showed characteristic trx active site at (96)wcgpc(100) and mitochondrial targeting peptide at the n- ... | 2008 | 18255328 |
| cdna cloning, characterization and expression analysis of the antioxidant enzyme gene, catalase, of chinese shrimp fenneropenaeus chinensis. | catalase is an important antioxidant protein that protects organisms against various oxidative stresses by eliminating hydrogen peroxide. the full-length catalase cdna of chinese shrimp fenneropenaeus chinensis was cloned from the hepatopancreas using degenerate primers by the method of 3' and 5' rapid amplification of cdna ends pcr. the cdna sequence consists of 1892 bp with a 1560 bp open reading frame, encoding 520 amino acids with high identity to invertebrate, vertebrate and even bacterial ... | 2008 | 18353680 |
| a novel c-type lectin from abalone, haliotis discus discus, agglutinates vibrio alginolyticus. | owing to its specific binding to carbohydrates, lectins play important roles in pathogen recognition and clearance in invertebrate animals. in this study, a novel c-type lectin (designated clhd) gene was isolated from abalone, haliotis discus discus, cdna library. the complete cdna sequence of the clhd gene is 508 base pairs in length, and encodes 151 amino acids. clhd shares a highly conserved carbohydrate recognition domain with c-type lectins from mollusk and fish. the mrna expressions of clh ... | 2008 | 18372041 |
| molecular characterization and expression analysis of regucalcin in disk abalone (haliotis discus discus): intramuscular calcium administration stimulates the regucalcin mrna expression. | regucalcin is a novel calcium (ca(2+)) binding protein and it has been demonstrated to play a multifunctional role in many organisms. here, we report the molecular cloning of invertebrate regucalcin cdna from disk abalone haliotis discus discus. the full length cdna showed 1321 bp of nucleotides with a polyadenylated sequence (aataaa). abalone regucalcin (hdreg) open reading frame (orf) consists of 918 nucleotides encoding 305 amino acids (aa). estimated molecular mass was 33 kda and predicted i ... | 2008 | 18372203 |
| the manganese superoxide dismutase gene in bay scallop argopecten irradians: cloning, 3d modelling and mrna expression. | a novel manganese superoxide dismutase (mnsod) was cloned from bay scallop argopecten irradians by 3' and 5' rapid amplification of cdna ends (race) pcr. the full-length cdna of mnsod was of 1207 bp with a 678 bp open reading frame encoding 226 amino acids. the deduced amino acid sequence contained a putative signal peptide of 26 amino acids. sequence comparison showed that the mnsod of a. irradians shared high identity with mnsod in invertebrates and vertebrates, such as mnsod from abalone hali ... | 2008 | 18674624 |
| molecular cloning and characterization of three sigma glutathione s-transferases from disk abalone (haliotis discus discus). | three novel glutathione s-transferase (gsts) cdnas were cloned from a disk abalone (haliotis dicus discus) cdna library. multiple alignment and phylogenetic analysis of three gsts revealed that their closest relationship is with insect sigma gsts. recombinant gsts were over-expressed in escherichia coli as soluble fusion proteins. hdgsts1 and hdgsts2 were active towards 1-chloro-2,4-dinitrobenzene and ethacrynic acid, whereas hdgsts3 appeared to be a non-enzymatic gst. two active gsts had simila ... | 2008 | 18703158 |
| transcriptional up-regulation of disk abalone selenium dependent glutathione peroxidase by h(2)o(2) oxidative stress and vibrio alginolyticus bacterial infection. | selenium dependent glutathione peroxidase (se-gpx) belongs to the family of selenoprotein, which acts mainly as an antioxidant in the cellular defence system. we have identified se-gpx full length cdna from disk abalone (haliotis discus discus) designated as abse-gpx. it has a characteristic codon at (223)tga(225) that corresponds to selenocysteine (sec) amino acid as u(75). the full length cdna consists of 675 bp, an open reading frame encoding 225 amino acids. sequence characterization reveale ... | 2008 | 18722791 |
| molecular characterization and gene expression analysis of a pattern recognition protein from disk abalone, haliotis discus discus. | pattern recognition molecules play an important role in innate immunity by recognizing common epitopes on the surface of invading microorganism. a pattern recognition protein (prp) was isolated from a disk abalone, haliotis discus discus, normalized cdna library. it encodes 420 amino acids (aa) including a 20 aa signal peptide sequence. the mature protein has an estimated molecular mass of 45kda and predicted pi of 5.0. the deduced aa sequence showed greatest homology (50%) with a beta-glucan re ... | 2008 | 18782621 |
| cloning and expression analysis of a small hsp26 gene of pacific abalone (haliotis discus hannai). | heat shock proteins (hsps) are evolutionally conserved from micro organism to mammals and play important roles in many biological processes including thermal tolerance. we isolated a homologue of small hsp26 (shsp26) from a subtracted cdna library of heat shock-treated abalone (haliotis discus hannai). the abalone shsp26 encompossed 793 nt, including a coding region of 501 nt. the deduced amino acid sequence of the abalone shsp26 contained well conserved alpha-crystallin domain and showed overal ... | 2008 | 19195400 |
| suppressor of cytokine signaling 2 (socs-2) homologue in disk abalone: cloning, sequence characterization and expression analysis. | suppressor of cytokine signaling (socs) proteins are inhibitors of cytokine signaling pathways and key physiological regulators of both innate and adaptive immune systems in vertebrates. in present study, we provided the initial characterization of socs-2 homologue from the mollusk abalone haliotis discus discus as a member of invertebrate socs-2. the disk abalone socs-2 cdna (absocs-2) contains 1700-bp full length with 771-bp coding sequence, which codes 257 amino acids protein. based on the se ... | 2009 | 19340953 |
| molecular cloning, characterization and expression analysis of peroxiredoxin 6 from disk abalone haliotis discus discus and the antioxidant activity of its recombinant protein. | peroxiredoxins (prxs) play an important role against various oxidative stresses and intra-cellular signal transduction. peroxiredoxin 6 (prxvi) was identified from the disk abalone haliotis discus discus cdna library and named hdprxvi. the full length cdna of hdprxvi was 1457 bp with a 654 bp open reading frame (orf) encoding 218 amino acids. the predicted molecular mass and estimated isoelectric point (pi) of hdprxvi were 24 kda and 7.3, respectively. the deduced amino acid sequence demonstrate ... | 2009 | 19460442 |
| a novel fas ligand in mollusk abalone: molecular characterization, immune responses and biological activity of the recombinant protein. | fas ligand is a member of the tnf superfamily that plays an important role by inducing apoptosis and homeostasis of immune responses. the gene encoding fas ligand was isolated from a disk abalone (haliotis discus discus) cdna library, denoted as the abfas ligand. it contains an 1832bp transcript with a 945bp open reading frame, encoding 315 amino acids. the abfas ligand showed characteristic transmembrane and tnf family signature domains. the deduced amino acid comparison showed that the abfas l ... | 2009 | 19576285 |
| molecular cloning, characterization, and the response of manganese superoxide dismutase from the antarctic bivalve laternula elliptica to pcb exposure. | manganese superoxide dismutase (lemnsod) cdna was cloned from the antarctic bivalve laternula elliptica. the full-length cdna of lemnsod is 1238 bp in length and contains an open reading frame of 681 bp encoding 226 amino acid residues including a putative mitochondrial targeting peptide of 26 amino acids in the n-terminal region. the calculated molecular mass is 24.8 kda with an estimated isoelectric point of 6.75. lemnsod signatures from 185 to 192 (dvwehayy) and four conserved amino acids (h5 ... | 2009 | 19628043 |
| wandonia haliotis gen. nov., sp. nov., a marine bacterium of the family cryomorphaceae, phylum bacteroidetes. | a novel, strictly aerobic, gram-stain-negative, yellow-orange-pigmented, rod-shaped bacterium was isolated from abalone (haliotis discus) under aquaculture in seawater off the wando coast, southern korea, and subjected to a polyphasic taxonomic study. cells of strain haldis-1(t) were catalase- and oxidase-positive rods with flexirubin pigments. phylogenetic analysis based on 16s rrna gene sequences revealed that strain haldis-1(t) formed a distinct lineage within the family cryomorphaceae and co ... | 2010 | 19654361 |
| novel omega glutathione s-transferases in disk abalone: characterization and protective roles against environmental stress. | omega glutathione s-transferases (gsts) are a newly identified class of gsts with unique properties compared to other members in gst superfamily. this present study reports the cloning, characterization and stress-induced expression analysis of two omega gst genes in disk abalone, haliotis discus discus. two disk abalone omega gst genes, hdgsto1 and hdgsto2, encode two polypeptides with calculated molecular mass of 27.4 and 26.9 kda, respectively. their deduced amino acid sequences showed highes ... | 2009 | 19689930 |
| transcriptional analysis of antioxidant and immune defense genes in disk abalone (haliotis discus discus) during thermal, low-salinity and hypoxic stress. | this study describes the transcriptional analysis of antioxidant and immune defense genes in gills tissue of disk abalone exposed to thermal, salinity and hypoxia-related stress, using quantitative real-time pcr. results showed that manganese superoxide dismutase (mnsod), copper zinc superoxide dismutase (cuznsod), catalase (cat), thioredoxin peroxidase (tpx), selenium dependant glutathione peroxidase (segpx), and thioredoxin-2 (trx-2) transcripts were expressed differently in gills, and that th ... | 2009 | 19699310 |
| abhisin: a potential antimicrobial peptide derived from histone h2a of disk abalone (haliotis discus discus). | antimicrobial peptides (amps) play an important role in the immune defense against pathogenic microorganisms. in this study, a histone h2a full-length cdna was cloned from disk abalone haliotis discus discus. we identified a 40-amino acid amp designated as abhisin from the n-terminus of the abalone histone h2a sequence. abhisin shows the characteristic features of amps including net positive charge (+13), higher hydrophobic residues (27%) and 2.82 kcal/mol protein binding potential. abhisin shar ... | 2009 | 19706329 |
| chitinase of bacillus licheniformis from oyster shell as a probe to detect chitin in marine shells. | bacillus licheniformis cbfos-03 is a chitinase producing bacteria isolated from oyster (crassostrea gigas) shell waste. we have cloned and expressed the chi18b gene of b. licheniformis cbfos-03, which encodes a glycohydrolase family 18 chitinase (gh18). chi18b is a predicted 598 amino acid protein that consists of a catalytic domain (gh18), a fibronectin type iii domain (fn3), and a chitin binding domain (cbd). purified chi18b showed optimum chitinase activity at ph 9 and 55 degrees c, and activ ... | 2010 | 19756579 |
| environmental determinants correlated to vibrio harveyi-mediated death of marine gastropods. | vibrio harveyi is an emerging pathogen that causes mass mortality in a wide variety of marine animal species; however, it is still unclear which environmental determinants correlate v. harveyi dynamics and the bacterium-mediated death of marine animal life. we conducted a correlation analysis over a 5-year period (2003-2007) analysing the following data: v. harveyi abundance, marine animal mortality and environmental variables (seawater temperature, salinity, ph, chlorophyll a, rainfall and tota ... | 2010 | 19758346 |
| defensin from disk abalone haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection. | gene-encoded antimicrobial peptides (amps) serve a major role in host defense systems against pathogens. in this study, cdna of a new mollusk defensin was identified from a normalized cdna library constructed from whole tissues of disk abalone. abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. the presence of an invertebrate defensin family domain, an arrangement of six cysteine ... | 2010 | 19922800 |
| disk abalone (haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: molecular cloning and immune response against bacterial infection. | a novel antistasin-like cdna homologue named as ab-antistasin was isolated from the disk abalone haliotis discus discus normalized cdna library. the ab-antistasin (1398-bp) consisted of an 1185-bp open reading frame encoding 395 amino acid (aa) residues. the predicted molecular mass and isoelectric point of ab-antistasin was 44 kda and 8.5, respectively, and showed highest identity (23.1%) to hydra magnipapillata antistasin. the most striking feature of ab-antistasin is the 12-fold internal repe ... | 2010 | 20060477 |
| molecular evidence for the existence of lipopolysaccharide-induced tnf-alpha factor (litaf) and rel/nf-kb pathways in disk abalone (haliotis discus discus). | the lipopolysaccharide-induced tnf-alpha factor (litaf) and rel family nuclear factor kappab (rel/nf-kb) are two important transcription factors which play major roles in the regulating inflammatory cytokine, apoptosis and immune related genes. here, we report the discovery of disk abalone litaf (ablitaf) and rel/nf-kb (abrel/nf-kb) homologues and their immune responses. full-length cdna of ablitaf consists of 441 bp open reading frame (orf) that translates into putative peptide of 147 aa. analy ... | 2010 | 20153832 |
| molecular cloning, characterization and mrna expression of selenium-dependent glutathione peroxidase from abalone haliotis discus hannai ino in response to dietary selenium, zinc and iron. | a novel selenium-dependent glutathione peroxidase (se-gpx) was cloned from abalone haliotis discus hannai ino (hdhgpx) by homology cloning with degenerate primers and race techniques. the full length of hdhgpx cdna was 963bp with a 669bp open reading frame (orf) encoding 222 amino acids and a 101bp eukaryotic selenocysteine insertion sequence (secis) in 3' untranslated region (utr). it was showed that hdhgpx has a characteristic codon at (235)tga(237) that corresponds to selenocysteine (sec) as ... | 2010 | 20348022 |
| characterization and expression analysis of ef hand domain-containing calcium-regulatory gene from disk abalone: calcium homeostasis and its role in immunity. | the complete amino acid sequence of a calcium-regulatory gene (denoted as ab-careg i) was identified from the disk abalone haliotis discus discus cdna library. the ab-careg i is composed of 176 amino acids and the calculated molecular mass and isoelectric point were 20 and 4.2, respectively. the sequence homology of ab-careg i was 28-30 and 18-27% of known calmodulin and troponin c, respectively. four characteristic calcium-binding ef hand motifs with some modifications at conserved positions of ... | 2010 | 20420919 |
| allograft inflammatory factor-1 in disk abalone (haliotis discus discus): molecular cloning, transcriptional regulation against immune challenge and tissue injury. | here, we report the identification and characterization of allograft inflammatory factor-1 (aif-1) from disk abalone haliotis discus discus that was denoted as abaif-1. the full-length cdna of abaif-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kda molecular mass. analysis of abaif-1 sequence showed that it shares characteristic two ef hand ca(+2)-binding motifs. results from phylogenetic analysis further confirm that abaif-1 is a member of the aif-1 family similar to inve ... | 2010 | 20435145 |
| tumor suppressor qm-like gene from disk abalone (haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge. | we describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor qm-like protein, abqm, in the disk abalone haliotis discus discus. the full-length cdna (765-bp) of abqm was found to consist of a 654-bp orf coding for a 218 amino acid protein of a 25 kda molecular mass with a 10.2 isoelectric point. analysis of abqm sequence revealed the presence of characteristic motifs, including the ribosomal protein l10 signature, sh3-binding motif and two antibiotic ... | 2010 | 20580829 |
| brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna from disk abalone haliotis discus discus. | a brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna was cloned from the disk abalone (haliotis discus discus) and designated as ablect-1. a full-length (705 bp) of ablect-1 cdna was composed of a 576 bp open reading frame that translates into a putative peptide of 192 amino acids. deduced amino acid sequence of ablect-1 had 15.5- and 27.8% identity and similarity to human lect-1, respectively. quantitative real-time pcr analysis results showed that the mrna of ablect-1 was ... | 2010 | 20688173 |
| biochemical and immunohistochemical analyses of a gnrh-like peptide in the neural ganglia of the pacific abalone haliotis discus hannai (gastropoda). | we examined whether gonadotropin-releasing hormone (gnrh)-like peptides are present in the neural ganglia of the gastropod pacific abalone (haliotis discus hannai) by reverse-phase high performance liquid chromatography (rphplc) combined with time-resolved fluoroimmunoassay (tr-fia) analysis and by immunohistochemistry. cerebral ganglion extracts showed a similar retention time to lamprey gnrh-ii (lgnrh-ii) in rphplc combined with tr-fia analysis. gnrh-like-immunoreactive (ir) cell bodies (which ... | 2010 | 20695781 |
| first molluscan transcription factor activator protein-1 (ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury. | the regulation of transcriptional activation is an essential and critical point in gene expression. in this study, we describe a novel transcription factor activator protein-1 (ap-1) gene from disk abalone haliotis discus discus (abap-1) for the first time in mollusk. it was identified by homology screening of an abalone normalized cdna library. the cloned abap-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. the abap-1 gene is composed of a charac ... | 2010 | 20732431 |
| molecular characterization of a mollusk chicken-type lysozyme gene from haliotis discus hannai ino, and the antimicrobial activity of its recombinant protein. | a chicken-type lysozyme was obtained and characterized from the abalone haliotis discus hannai ino (hdlysc). the full-length cdna of hdlysc was 586 bp, and it contained an open reading frame of 441 bp, encoding a 147-amino acid protein with a calculated molecular mass of 15.64 kda and an isoelectric point of 4.87. the amino acid sequence of hdlysc possessed all conserved features critical for the fundamental structure and function of c-type lysozymes, including the two catalytic residues, glu54 ... | 2010 | 20951809 |
| expression and localization of mcsialec, a sialic acid-specific lectin in the marine bivalve manila clam, ruditapes philppinarum. | a novel sialic acid-specific lectin (mcsialec) was detected from an expressed sequenced tag (est) sequence from manila clam haemocytes infected with perkinsus olseni. the cdna of the lectin was cloned using gene-specific primers based on a previously determined est and characterized. the full-length cdna of mcsialec is 603 bp in length and encodes a polypeptide of 200 amino acids with a calculated molecular mass of 21.928 kda. sequence alignment and protein motif analyses showed that mcsialec sh ... | 2010 | 21039608 |
| molluscan death effector domain (ded)-containing caspase-8 gene from disk abalone (haliotis discus discus): molecular characterization and expression analysis. | the caspase family represents aspartate-specific cysteine proteases that play key roles in apoptosis and immune signaling. in this study, we cloned the first death effector domain (ded)-containing molluscan caspase-8 gene from disk abalone (haliotis discus discus), which is named as hdcaspase-8. the full-length hdcaspase was 2855 bp, with a 1908 bp open reading frame encoding 636 amino acids. the hdcaspase-8 had 72 kda predicted molecular mass with an estimated isoelectric point (pi) of 6.0. the ... | 2010 | 21130887 |
| microarray analysis of gene expression in disk abalone haliotis discus discus after bacterial challenge. | in this study, we investigated the gene expression profiling of disk abalone, haliotis discus discus challenged by a mixture of three pathogenic bacteria vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes using a cdna microarray. upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels ≥2 or ≤2 -fold in gills and digestive tract, respectively. there were 46 tissue-specific transcripts that up-regulated specifically in the digestive ... | 2011 | 21215803 |
| effects of dietary zinc on gene expression of antioxidant enzymes and heat shock proteins in hepatopancreas of abalone haliotis discus hannai. | the expression patterns of different genes encoding antioxidant enzymes and heat shock proteins were investigated, in present study, by real-time quantitative pcr in the hepatopancreas of abalone haliotis discus hannai fed with different levels of dietary zinc (6.69, 33.8, 710.6 and 3462.5 mg/kg) for 20 weeks. the antioxidant enzymes include cu/zn-superoxide dismutase (cu/zn-sod), mn-superoxide dismutase (mn-sod), catalase (cat), mu-glutathione-s-transferase (mu-gst) and thioredoxin peroxidase ( ... | 2011 | 21406247 |
| transcriptional analysis of disk abalone (haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge. | diverse antioxidant enzymes are essential for marine organisms to overcome oxidative stress as well as for the fine-tuning of immune reactions through activating different signal transduction pathways. this study describes the transcriptional analysis of antioxidant enzymes of disk abalone by challenging with bacteria (vibrio alginolyticus, vibrio parahemolyticus, and listeria monocytogenes) and viral hemorrhagic septicemia virus (vhsv). upon bacteria and vhsv challenge, manganese superoxide dis ... | 2011 | 21549200 |
| permanent genetic resources added to molecular ecology resources database 1 june 2010 - 31 july 2010. | this article documents the addition of 205 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: bagassa guianensis, bulweria bulwerii, camelus bactrianus, chaenogobius annularis, creontiades dilutus, diachasmimorpha tryoni, dioscorea alata, euhrychiopsis lecontei, gmelina arborea, haliotis discus hannai, hirtella physophora, melanaphis sacchari, munida isos, thaumastocoris peregrinus and tuberolachnus salignus. these loci were cro ... | 2010 | 21565125 |
| cdna cloning and mrna expression of a tandem-repeat galectin (pogal2) from the pearl oyster, pinctada fucata. | galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. we cloned the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated as pogal2). pogal2 cdna is 1347 bp long and consists of a 5'-untranslated region (utr) of 3 bp, a 3'-utr of 297 bp with one cytokine rna instability motif (attta), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, wi ... | 2011 | 21948759 |
| formation of the cylindrical structure during the acrosome reaction of abalone spermatozoa. | spermatozoa of abalone haliotis discus were examined before and during the acrosome reaction with special regard to one of the newly formed structures: a cylindrical structure surrounding a part of the elongated acrosomal process near the opening of the acrosomal vesicle. the structure, about 0.2 micron in diameter and about 1 micron in length, was revealed to be composed of a tightly coiled, fine tubular structure about 20 nm in diameter. in the course of the acrosome reaction, a triple-spiral ... | 2012 | 3506898 |
| actinomyces haliotis sp. nov., a bacterium isolated from the gut of an abalone, haliotis discus hannai. | a novel, gram-staining-positive, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain wl80(t), was isolated from the gut of an abalone, haliotis discus hannai, collected from the northern coast of jeju in korea. optimal growth occurred at 30 °c, ph 7-8 and with 1% (w/v) nacl. phylogenetic analyses based on the 16s rrna gene sequence revealed that strain wl80(t) fell within the cluster of the genus actinomyces, with highest sequence similarity to the type strains of actinomyces ... | 2014 | 24096355 |
| antimicrobial peptide, hdmolluscidin, purified from the gill of the abalone, haliotis discus. | a 4.7 kda antimicrobial peptide was purified from the acidified gill extract of the abalone, haliotis discus, by cation-exchange and c18 reversed-phase high performance liquid chromatography (hplc). comparison of the amino acid sequences and molecular weight of this peptide with those of other known antimicrobial peptides revealed that this antimicrobial peptide have high sequence homology with that of cgmolluscidin and was designated hdmolluscidin. hdmolluscidin is composed of 46 amino acid res ... | 2016 | 27033467 |
| ferritin from the pacific abalone haliotis discus hannai: analysis of cdna sequence, expression, and activity. | ferritin plays an important role in iron homeostasis due to its ability to bind and sequester large amounts of iron. in this study, the gene encoding a ferritin (hdhfer2) was cloned from pacific abalone (haliotis discus hannai). the full-length cdna of hdhfer2 contains a 5'-utr of 121 bp, an orf of 516 bp, and a 3'-utr of 252 bp with a polyadenylation signal sequence of aataaa and a poly(a) tail. it also contains a 31 bp iron-responsive element (ire) in the 5'-utr position, which is conserved in ... | 2016 | 26766182 |
| molecular insights into a molluscan transferrin homolog identified from disk abalone (haliotis discus discus) evidencing its detectable role in host antibacterial defense. | the basic function of transferrin is to bind iron (iii) ions in the medium and to deliver them to the locations where they are required for metabolic processes. it also takes part in the host immune defense mainly via its ability to bind to iron (iii) ions. hence, transferrin is also identified as an important acute-phase protein in host immunity. abalones are major shellfish aquaculture crops that are susceptible to a range of marine microbial infections. since transferrin is known to be a majo ... | 2015 | 26191782 |
| hdhctl1 is a novel c-type lectin of abalone haliotis discus hannai that agglutinates gram-negative bacterial pathogens. | c-type lectins (ctls) are ca(2+)-dependent carbohydrate recognition proteins, which play important roles in the innate immunity of both vertebrates and invertebrates. in this study, we identified and characterized a c-type lectin (named hdhctl1) from pacific abalone, haliotis discus hannai. hdhctl1 is composed of 176 amino acid residues and shares low (23.9%) identity with the known ctl of abalone. hdhctl1 possesses a putative signal peptide and a carbohydrate-recognition domain (crd) typical of ... | 2014 | 25301718 |
| evidences for the involvement of an invertebrate goose-type lysozyme in disk abalone immunity: cloning, expression analysis and antimicrobial activity. | lysozymes are ubiquitously distributed enzymes with hydrolytic activity against bacterial peptidoglycan and function to protect organisms from microbial pathogens. in this study, an invertebrate goose-type lysozyme, designated as ablysg, was identified in the disk abalone, haliotis discus discus. the full-length cdna of ablysg was 894 bp in length with an open reading frame of 789 bp encoding a polypeptide of 263 amino acids containing a signal peptide and a characteristic soluble lytic transgly ... | 2013 | 23973847 |
| expression characterization and activity analysis of a cathepsin b from pacific abalone haliotis discus hannai. | cathepsin b (ec 3.4.22.1) is a member of lysosomal cysteine protease and has a papain-like fold. in mammals, it is involved in protein degradation and other physiological processes including immune response. however, little is known about the function of cathepsin b in mollusks. in this study, we identified and analyzed a cathepsin b homolog (hdcatb) from pacific abalone (haliotis discus hannai), an economically important mollusk species cultured in east asia. hdcatb is composed of 336 amino aci ... | 2013 | 23473863 |
| molecular and functional characterization of hdhsp20: a biomarker of environmental stresses in disk abalone haliotis discus discus. | heat shock proteins (hsps) production in cell is inducible by many physical and chemical stressors, providing adaptive significance for organisms when faced with environmental changes. in this study, we characterized a novel small hsp gene from disk abalone, designated as hdhsp20, and investigated its temporal expression by different environmental stimuli. the full-length genome sequence of hdhsp20 is composed of three exons and two introns. the 5' flanking region contains multiple putative tran ... | 2012 | 22498576 |
| recombinant perlucin nucleates the growth of calcium carbonate crystals: molecular cloning and characterization of perlucin from disk abalone, haliotis discus discus. | perlucin is well known as an important functional protein regulating pearl formation and shell biomineralization. in this study, we cloned the perlucin gene from the abalone haliotis discus discus cdna library. the full-length cdna of the abalone h. discus discus perlucin gene consisted of 1038 bp nucleotides, encoding a putative signal peptide of 22 amino acids and a mature protein of 129 amino acids, which shared 55% identity with the homologous protein in greenlip abalone. the mature protein ... | 2008 | 18068384 |
| analysis of microbiota on abalone (haliotis discus hannai) in south korea for improved product management. | abalone is a popular seafood in south korea; however, because it contains various microorganisms, its ingestion can cause food poisoning. therefore, analysis of the microbiota on abalone can improve understanding of outbreaks and causes of food poisoning and help to better manage seafood products. in this study, we collected a total of 40 abalones from four different regions in march and july, which are known as the maximum abalone production areas in korea. the microbiota were analyzed using hi ... | 2016 | 27371902 |
| molecular identification and functional delineation of a glutathione reductase homolog from disk abalone (haliotis discus discus): insights as a potent player in host antioxidant defense. | glutathione reductase (gsr) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (gssg) into the reduced form (gsh). since the ratio between the two forms of glutathione (gsh/gssg) is important for the optimal function of gsh to act as an antioxidant against h2o2, the contribution of gsr as an enzymatic regulatory agent to maintain the proper ratio is essential. abalones are marine mollusks that frequently encounter environmental factors that can trigger the overproduct ... | 2017 | 27919756 |
| three novel c1q domain containing proteins from the disk abalone haliotis discus discus: genomic organization and analysis of the transcriptional changes in response to bacterial pathogens. | the globular c1q (gc1q) domain containing proteins, commonly referred as c1q domain containing (c1qdc) proteins, are an essential family of proteins involved in various innate immune responses. in this study, three novel c1qdc proteins were identified from the disk abalone (haliotis discus discus) transcriptome database and designated as abc1qdc1, abc1qdc2, and abc1qdc3. the cdna sequences of abc1qdc1, abc1qdc2, and abc1qdc3 consisted of 807, 1305, and 660 bp open reading frames (orfs) encoding ... | 2016 | 27417231 |
| a molluscan calreticulin ortholog from haliotis discus discus: molecular characterization and transcriptional evidence for its role in host immunity. | calreticulin (calr), a ca(2+) binding chaperone of the endoplasmic reticulum (er) and mainly involved in ca(2+) storage and signaling. in this study, we report the molecular characterization and immune responses of calr homolog from disk abalone (abcalr). the full length abcalr cdna (1837 bp) had an orf of 1224 bp. according to the multiple alignments analysis, n- and p-domains were highly conserved in all the selected members of calrs. in contrast, the c-domain which terminated with the charact ... | 2016 | 27086846 |
| an invertebrate signal transducer and activator of transcription 5 (stat5) ortholog from the disk abalone, haliotis discus discus: genomic structure, early developmental expression, and immune responses to bacterial and viral stresses. | signal transducer and activator of transcription (stat) family members are key signaling molecules that transduce cellular responses from the cell membrane to the nucleus upon janus kinase (jak) activation. although seven stat members have been reported in mammals, very limited information on stat genes in molluscans is available. in this study, we identified and characterized a stat paralog that is homologous to stat5 from the disk abalone, haliotis discus discus, and designated as abstat5. com ... | 2016 | 26616564 |
| two variants of selenium-dependent glutathione peroxidase from the disk abalone haliotis discus discus: molecular characterization and immune responses to bacterial and viral stresses. | glutathione peroxidase (gpx) is an essential member of the antioxidant systems of living organisms and may be involved in immune defense against pathogenic invasion. in the current study, two selenium-dependent glutathione peroxidases (absegpxs) that shared 54.3% identity were identified from the disk abalone haliotis discus discus. the open reading frames (orfs) of absegpx-a and absegpx-b coded for 222 and 220 amino acids, respectively, with a characteristic selenocysteine residue encoded by an ... | 2015 | 26025184 |
| three novel clade b serine protease inhibitors from disk abalone, haliotis discus discus: molecular perspectives and responses to immune challenges and tissue injury. | serine protease inhibitors (serpins) control cellular protease activity in order to maintain cellular homeostasis. the immune and inflammatory responses of invertebrate clade b serpins have not been widely reported. in the present study, three proteins with high similarity to clade b serpins, referred to as abserpin-1, abserpin-2 and abserpin-3, were identified from disk abalone (haliotis discus discus). while abserpin-1 (399 aa) was of a typical size for this protein class, abserpin-2 (506 aa) ... | 2015 | 25917971 |
| antibacterial activity and immune responses of a molluscan macrophage expressed gene-1 from disk abalone, haliotis discus discus. | the membrane-attack complex/perforin (macpf) domain-containing proteins play an important role in the innate immune response against invading microbial pathogens. in the current study, a member of the macpf domain-containing proteins, macrophage expressed gene-1 (mpeg1) encoding 730 amino acids with the theoretical molecular mass of 79.6 kda and an isoelectric point (pi) of 6.49 was characterized from disk abalone haliotis discus discus (abmpeg1). we found that the characteristic macpf domain (v ... | 2014 | 24852343 |
| a bifunctional invertebrate-type lysozyme from the disk abalone, haliotis discus discus: genome organization, transcriptional profiling and biological activities of recombinant protein. | lysozyme is an important enzyme in the innate immune system that plays a vital role in fighting microbial infections. in the current study, we identified, cloned, and characterized a gene that encodes an invertebrate-type lysozyme from the disk abalone, haliotis discus discus (ablysi). the full-length cdna of ablysi consisted of 545 bp with an open reading frame of 393 bp that encodes 131 amino acids. the theoretical molecular mass of mature ablysi was 12.3 kda with an isoelectric point of 8.03. ... | 2013 | 23796790 |
| molluskan fasciclin-1 domain-containing protein: molecular characterizationand gene expression analysis of fasciclin 1-like protein from disk abalone (haliotis discus discus). | cell-to-cell contacts play a key role in multicellular systems and organisms. fasciclin-1 (fas-1) is a lipid-linked membrane associated glycoprotein that is a member of a newly recognized family of cell adhesion molecules sharing features with the immunoglobulins, cadherins, integrins, and selectins. here, we report the identification and molecular characterization of a novel fas-1 domain-containing cdna from disk abalone (haliotis discus discus), including its gene expression profile and immune ... | 2013 | 23562785 |
| polaribacter haliotis sp. nov., isolated from the gut of abalone haliotis discus hannai. | a gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated ra4-7t, was isolated from the gut of an abalone (haliotis discus hannai) collected from the sea around jeju island, south korea, and subjected to a polyphasic taxonomic study. ra4-7t grew optimally at 25 °c, at ph 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) nacl. the phylogenetic trees based on 16s rrna gene sequences showed that ra4-7t represented a member of the genus polaribacter. ra4-7 ... | 2016 | 27902190 |
| the role of hybridization in improving the immune response and thermal tolerance of abalone. | recently, frequent death of cultured abalone drew our attention to the stress tolerance of abalone. hybridization is an effective way of genetic improvement in aquaculture, which can introduce improved traits to the hybrids. in this study, we challenged the hybrids between haliotis discus hannai and haliotis gigantea, and their parents with bacteria (vibrio harveyi, vibrio alginolyticus and vibrio parahemolyticus), then held them at 20 °c and 28 °c, survival rates of the parental populations and ... | 2014 | 24794582 |
| structural characterization and expression analysis of a novel cysteine protease inhibitor from haliotis discus hannai ino. | the sequence of the cysteine protease inhibitor gene of haliotis discus hannai (designated hdcpi) was determined using the race method. the full-length hdcpi cdna is 1049 bp long, and contains an open reading frame of 813 bp, encoding a 271-amino-acid protein with a calculated molecular mass of 29.83 kda and an isoelectric point of 8.57. the deduced amino acid sequence of hdcpi contains two cystatin-like domains, and each has the structural features of the cystatin family, including three evolut ... | 2015 | 25463299 |
| effects of dietary supplementation of probiotic shewanella colwelliana wa64, shewanella olleyana wa65 on the innate immunity and disease resistance of abalone, haliotis discus hannai ino. | the effects of dietary administration of two probiotics, shewanella colwelliana wa64 and shewanella olleyana wa65, on the innate immunity of abalone (haliotis discus hannai ino), and survival of juvenile abalone challenged with vibrio harveyi have been studied. two groups of abalone were fed with three different diets: one control, and two diets supplemented with 10(9) cell g(-1) of probiotic wa64 (wa64 diet) and wa65 (wa65 diet) for up to four weeks. results showed that abalone fed diets contai ... | 2013 | 23602848 |
| cathepsin l is an immune-related protein in pacific abalone (haliotis discus hannai)--purification and characterization. | cathepsin l, an immune-related protein, was purified from the hepatopancreas of pacific abalone (haliotis discus hannai) by ammonium sulfate precipitation and column chromatographies of sp-sepharose and sephacryl s-200 hr. purified cathepsin l appeared as two bands with molecular masses of 28.0 and 28.5 kda (namely cathepsin la and lb) on sds-page under reducing conditions, suggesting that it is a glycoprotein. peptide mass fingerprinting (pmf) analysis revealed that peptide fragments of 95 amin ... | 2015 | 26549175 |
| molecular insights of the first gastropod tlr counterpart from disk abalone (haliotis discus discus), revealing its transcriptional modulation under pathogenic stress. | toll-like receptors (tlrs) are well-characterized pattern recognition receptors of innate immunity, known to induce immune responses against the pathogens by interacting with evolutionarily conserved pathogen-associated molecular patterns (pamps). in this study, a novel tlr homolog from disk abalone (haliotis discus discus) was identified and characterized at molecular level. the open reading frame (orf) of abtlr is 3804 bp in length and encodes a 1268 amino acid peptide with a calculated molecu ... | 2013 | 23669649 |
| identification of a novel molluscan short-type peptidoglycan recognition protein in disk abalone (haliotis discus discus) involved in host antibacterial defense. | peptidoglycan recognition proteins (pgrps) are a widely studied group of pattern recognition receptors found in invertebrate as well as vertebrate lineages, and are involved in bacterial pathogen sensing. however, in addition to this principal role, they can also function in multiple host defense processes, including cell phagocytosis and hydrolysis of peptidoglycans (pgns). in this study, a novel invertebrate short-type pgrp was identified in disk abalone (haliotis discus discus) designated as ... | 2014 | 24811007 |
| kazal-type proteinase inhibitor from disk abalone (haliotis discus discus): molecular characterization and transcriptional response upon immune stimulation. | proteinases and proteinase inhibitors are involved in several biological and physiological processes in all multicellular organisms. proteinase inhibitors play a key role in regulating the activity of the respective proteinases. among serine proteinase inhibitors, kazal-type proteinase inhibitors (kpis) are widely found in mammals, avians, and a variety of invertebrates. in this study, we describe the identification of a kazal-type serine proteinase inhibitor (ab-kpi) from the disk abalone, hali ... | 2013 | 23859879 |
| cdna microarray analysis of disk abalone genes in gills and hemocytes after viral hemorrhagic septicemia virus (vhsv) challenge. | a disk abalone haliotis discus discus 4.2 k cdna microarray was designed by selecting abalone expressed sequence tags (ests). transcriptional profiles in gills and hemocytes were analyzed upon abalone challenged with viral hemorrhagic septicemia virus (vhsv) in order to select candidates for screening of immune response genes. among the 4188 genes analyzed, 280 (6.6%) transcripts were changed their expression level in gills and hemocytes against vhsv challenge compared to control animals. total ... | 2012 | 22465362 |
| recovery of coastal fauna after the 2011 tsunami in japan as determined by bimonthly underwater visual censuses conducted over five years. | massive tsunamis induce catastrophic disturbance in marine ecosystems, yet they can provide unique opportunities to observe the process of regeneration. here, we report the recovery of fauna after the 2011 tsunami in northeast japan based on underwater visual censuses performed every two months over five years. both total fish abundance and species richness increased from the first to the second year after the tsunami followed by stabilization in the following years. short-lived fish, such as th ... | 2016 | 27942028 |
| effects of traditional chinese medicine on immune responses in abalone, haliotis discus hannai ino. | a traditional chinese medicine (tcm) preparation was formulated from orange peel (pericarpium citri reticulatae), hawthorn (crataegus pinnatifida), astragalus (astragalus membranaceus (fisch.) bunge), pilose asiabell root (radix codonopsis), indigowoad root (radix isatidis), taraxacum (herba taraxaci) and malt (fructus hordei germinatus) at a weight ratio of 1:1:1.5:1.5:1.5:1.5:2. a feeding experiment was conducted to determine the effects of tcm on innate immunity of abalone, haliotis discus ha ... | 2008 | 18378466 |
| cloning, characterization and tissue expression of disk abalone (haliotis discus discus) catalase. | catalase is an antioxidant enzyme that plays a significant role in protection against oxidative stress by detoxification of hydrogen peroxide (h2o2). a gene coding for a putative catalase was isolated from the disk abalone (haliotis discus discus) cdna library and denoted as ab-catalase. the full-length (2864 bp) ab-catalase cdna contained 1,503 bp open reading frame (orf), encoding 501 amino acid residues with 56 kda predicted molecular weight. the deduced amino acid sequence of ab-catalase has ... | 2008 | 18187341 |
| high expression of new genes in trochophore enlightening the ontogeny and evolution of trochozoans. | animals with trochophore larvae belong to trochozoa, one of the main branches of bilateria. in addition to exhibiting spiral cleavage and early cell fate determination, trochozoans typically undergo indirect development, which contributes to the most unique characteristics of their ontogeny. the indirect development of trochozoans has provoked discussion regarding the origin and evolution of marine larvae and is interesting from the perspective of phylogeny-ontogeny correspondence. while these p ... | 2016 | 27698463 |
| molecular cloning and characterization of socs-2 from manila clam ruditapes philippinarum. | suppressor of cytokine signaling (socs) family members are key regulators of immunological homeostasis. in this study, we have discovered the socs-2 member from manila clam ruditapes philippinarum and further analyzed its immune responses against lipopolysaccharide (lps) and polyinosinic:polycytidylic acid (poly i:c). amino acid sequence of rpsocs-2 consists of cytokine inducible src homology 2 (sh2) and socs box domains similar to vertebrate socs counterparts. it has the highest amino acid iden ... | 2014 | 24378684 |
| genomic characterization and expression profiles upon bacterial infection of a novel cystatin b homologue from disk abalone (haliotis discus discus). | cystatins are a large family of cysteine proteinase inhibitors which are involved in diverse biological and pathological processes. in the present study, we identified a gene related to cystatin superfamily, abcyt b, from disk abalone haliotis discus discus by expressed sequence tag (est) analysis and bac library screening. the complete cdna sequence of abcyt b is comprised of 1967 nucleotides with a 306 bp open reading frame (orf) encoding for 101 amino acids. the amino acid sequence consists o ... | 2012 | 22878425 |
| transcriptional up-regulation of a novel ferritin homolog in abalone haliotis discus hannai ino by dietary iron. | a novel cdna encoding ferritin (hdhnft) was cloned from the hepatopancreas of abalone, haliotis discus hannai ino. the deduced protein contains 171 amino acid residues with a predicted molecular mass (mw) about 19.8 kda and theoretical isoelectric point (pi) of 4.792. amino acid alignment revealed that hdhnft shared high similarity with other known ferritins. the hdhnft contained a highly conserved motif for the ferroxidase center, which consists of seven residues of a typical vertebrate heavy-c ... | 2010 | 20647051 |
| marine lectins dlfbl and hddsbl fused with soluble coxsackie-adenovirus receptor facilitate adenovirus infection in cancer cells but have different effects on cell survival. | cancer development and progression are usually associated with glycosylation change, providing prognostic and diagnostic biomarkers, as well as therapeutic targets, for various cancers. in this work, dicentrarchus labrax fucose binding lectin (dlfbl) and haliotis discus discus sialic acid binding lectin (hddsbl) were genetically fused with soluble coxsackie-adenovirus receptor (scar), and produced through a bacterial expression system. results showed that recombinant scar-dlfbl not only facilita ... | 2017 | 28335432 |
| lectins with potential for anti-cancer therapy. | this article reviews lectins of animal and plant origin that induce apoptosis and autophagy of cancer cells and hence possess the potential of being developed into anticancer drugs. apoptosis-inducing lectins encompass galectins, c-type lectins, annexins, haliotis discus discus lectin, polygonatum odoratum lectin, mistletoe lectin, and concanavalin a, fucose-binding dicentrarchus labrax lectin, and strongylocentrotus purpuratus lectin, polygonatum odoratum lectin, and mistletoe lectin, polygonat ... | 2015 | 25730388 |
| two duplicated chicken-type lysozyme genes in disc abalone haliotis discus discus: molecular aspects in relevance to structure, genomic organization, mrna expression and bacteriolytic function. | lysozymes are crucial antibacterial proteins that are associated with catalytic cleavage of peptidoglycan and subsequent bacteriolysis. the present study describes the identification of two lysozyme genes from disc abalone haliotis discus discus and their characterization at sequence-, genomic-, transcriptional- and functional-levels. two cdnas and bac clones bearing lysozyme genes were isolated from abalone transcriptome and bac genomic libraries, respectively and sequences were determined. cor ... | 2013 | 23664908 |
| genome sequence of arcobacter sp. strain la11, isolated from the abalone haliotis discus. | arcobacter sp. strain la11 was isolated from the gut of the abalone haliotis discus here, we present the annotation and analysis of the draft genome of this strain, which is involved in nitrogen metabolism. | 2017 | 28302779 |