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neuroprotective effects of a standardized extract of diospyros kaki leaves on mcao transient focal cerebral ischemic rats and cultured neurons injured by glutamate or hypoxia.naoxinqing (nxq, a standardized extract of diospyros kaki leaves) is a patented and approved drug of traditional chinese medicine (tcm) used for the treatment of apoplexy syndrome for years in china, but its underlying mechanism remains to be further elucidated. the present study investigates the effects of nxq against focal ischemia/reperfusion injury induced by middle cerebral artery occlusion (mcao) in rats and against glutamate-induced cell injury of hippocampal neurons as well as against hy ...200717583826
antibacterial activity of plants used in cooking for aroma and taste.thirty-three plants used in cooking for aroma and taste were examined for antibacterial activity against pathogens causing foodborne infections. vibrio parahaemolyticus and staphylococcus aureus were sensitive to many kinds of plant extracts, whereas listeria monocytogenes, escherichia coli o157:h7, and salmonella enteritidis populations decreased in only six, one, and three plant extracts, respectively. the polyphenol content in the plants was significantly different between the antibacterial p ...200415633695
the anticoagulant fraction from the leaves of diospyros kaki l. has an antithrombotic activity.the leaves of persimmon (diospyros kaki l.) has long been used for tea in korea since it was thought to be effective against hypertension. an anticoagulant fraction was purified through gel filtration g-100, hydrophobic, gel filtration g-150, and fplc, phenyl superpose column chromatographies. the purified fraction was homogenous and its mr was estimated 10,000 da by gel filtration and sds-page. the purified fraction was sensitive to treatment of subtilisin b, but not to heat and its activity wa ...200516042075
[effects of flavone from leaves of diospyros kaki on expression of apoptosis signal-regulating kinase 1 and rat vascular smooth muscle cells proliferation by tumor necrosis factor alpha in vitro].to observe the effects of flavone from leaves of diospyros kaki on expression of apoptosis signal-regulating kinase 1 (ask1) and rat vascular smooth muscle cells (vsmcs) proliferation by tumor necrosis factor alpha in vitro.200717944194
management of sah with traditional chinese medicine in china.china lacks large scale authorized epidemiological study results in allusion to subarachnoid hemorrhage (sah) within recent 15 years since monica (multinational monitoring of trends and determinants in cardiovascular disease) study revealed sah situation in china in 2000. the main cause of sah in china is aneurysm which takes up 30-50%, while over 90% aneurysm locates at willis circle. early surgery for sah after aneurysm rupture is the dominant procedure to deal with sah in china. moreover, cal ...200616759447
screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, dpph radical scavenging assay, and folin-ciocalteu assay.oxidation of low-density lipoprotein (ldl) has been implicated in atherogenesis. antioxidants that prevent ldl from oxidizing may reduce atherosclerosis. this study investigated ldl antioxidant activity in edible plant products for development of dietary supplementation to prevent atherosclerosis. fifty-two kinds of edible plants were extracted using 70% aqueous ethanol solution, and the antioxidant activity of the extracts, which inhibit human ldl oxidation induced by copper ion, was determined ...200415080652
[effects of flavone from leaves of diospyros kaki on rat vascular smooth muscle cells proliferation stimulated by native low-density lipoprotein in vitro].to observe whether rat vascular smooth muscle cells (vsmcs) proliferation induced by native low-density lipoprotein (ldl) is affected by flavone from leaves of diospyros kaki in vitro.200415658826
[flavonoids from diospyros kaki inhibit the adhesion between lymphocyte and dorsal root ganglion].to investigate the effects of flavonoids from the leaves of diospyros kaki l (fldk) on the adhesion between the lymphocyte and the neurone.200919771850
native and introduced host plants of anastrepha fraterculus and ceratitis capitata (diptera: tephritidae) in northwestern argentina.wild or commercially grown, native and exotic fruit were collected in 30 localities in the tucumán province (nw argentina) from january 1990 to december 1995 to determine their status as hosts of anastrepha fraterculus (wiedemann) and/or ceratitis capitata (wiedemann), the only two fruit fly species of economic and quarantine importance in argentina. a total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. we determined the followin ...200314503581
in vitro effects of flavone from leaves of diospyros kaki on rat cardiac myocyte apoptosis induced by hypoxia-reoxygenation and advanced glycation end products.to investigate the effects of flavone extracted from the leaves of diospyros kaki on the apoptosis of rat cardiac myocytes induced by hypoxia-reoxygenation and advanced glycation end products in vitro.200312865219
[changes in cell wall component metabolism and ultrastructure of postharvest persimmon fruit during softening].the changes in cellular wall hydrolases, cellular wall components and cellular wall ultrastructure of postharvest persimmon (diospyros kaki l. cv. bianhua) fruit during softening were studied. pectinesterase activity increased sharply at first and reached a peak (fig.3a). significant correlation was observed between the pectinesterase activity and the loss of flesh firmness (r= -0.74). polygalacturonase activity increased slowly (fig.3b), but there was no significant correlation between the poly ...200516361794
biological activity of persimmon (diospyros kaki) peel extracts.fractionated extracts of persimmon (diospyros kaki) peels were studied for cytotoxic activity, multidrug resistance (mdr) reversal activity, anti-human immunodeficiency virus (hiv) activity and anti-helicobacter pylori (h. pylori) activity. the potent cytotoxic activity against human oral squamous cell carcinoma cells (hsc-2) and human submandibular gland tumor (hsg) cells was found in the acetone fractions (a4 and a5) with ic(50) ranging from 21 to 59 micro g/ml. however, the cytotoxic activity ...200312748986
effect of heat treatment on the antioxidative and antigenotoxic activity of extracts from persimmon (diospyros kaki l.) peel.heat treatment of persimmon peel (pp) increased the antioxidative activity of the 70% ethanolic extract (ee) and water extract (we) from pp. ee and we both prevented h2o2-induced dna damage to human peripheral lymphocytes. the antioxidative and antigenotoxic activities of the pp extracts were significantly affected by heating.200616636469
identification of a new apscaviroid from japanese persimmon.three viroid-like sequences were detected from japanese persimmon (diospyrus kaki thunb.) by rt-pcr using primers specific for members of the genus apscaviroid. based on the sequences, we determined the complete genomic sequences. two had 92.1-94.3% sequence identity with citrus viroid os (cvd-os) and 91.4-96.3% identity with apple fruit crinkle viroid (afcvd), respectively. another one, tentatively named persimmon viroid (pvd), had 396 nucleotides and less than 70% sequence identity with known ...200818365126
pheophorbide a-methyl ester, acyl-coa: cholesterol acyltransferase inhibitor from diospyros kaki.in the course of our search for acyl-coa: cholesterol acyltransferase (acat) inhibitors from natural sources, a new type of acat inhibitor was isolated from a methanol extract of diospyros kaki. on the basis of spectral and structural evidence, the compound was identified as pheophorbide a-methyl ester. pheophorbide a-methyl ester inhibited acat activity in a dose dependent manner with an ic50 value of 1.85 microg/ml.200314560918
incidence and growth of listeria monocytogenes in persimmon (diospyros kaki) fruit.the incidence of listeria monocytogenes on persimmon (diospyros kaki) surface of 'fuyu' and 'rama forte' was evaluated during a 5-month-period (from march to july) of two seasons periods (years 2005 and 2006). the fruits were collected in wholesale and street markets and retail in sao paulo and campinas city, brazil. a total of 582 fruits were analyzed using the bax system which is based on the polymerase chain reaction. the ability of this pathogen to grow on the peel and pulp of the two persim ...200818599141
bile acid-binding activity of young persimmon (diospyros kaki) fruit and its hypolipidemic effect in mice.the hypolipidemic effects and bile acid-binding properties of young persimmon (diospyros kaki) fruit were examined. in an animal experiment, male c57bl/6.cr mice (n = 5) were fed an ain-76-modified high fat diet supplemented with 2% or 5% (w/w) dried young persimmon fruit (yp) for 10 weeks. the intake of yp significantly enhanced fecal bile acid excretion and lowered the concentration of hepatic lipids and plasma cholesterol. analysis of gene expression in liver tissue showed that 2% or 5% yp up ...201019585467
inhibitory effects of flavonoid glycosides isolated from the peel of japanese persimmon (diospyros kaki 'fuyu') on melanin biosynthesis.we found that the acetone extract of the peel of japanese persimmon (diospyros kaki 'fuyu') inhibits melanin biosynthesis in mouse b16 melanoma cells. the activity-guided purification of the extract resulted in isolation of two active compounds, which have been identified as flavonoid glycosides, isoquercitrin (quercetin-3-o-glucoside) and hyperin (quercetin-3-o-galactoside) by spectral analysis. isoquercitrin and hyperin strongly inhibited the production of melanin (ic(50): 21.7 and 18.2 microm ...201020045948
inhibition of enzyme activities and the antiwrinkle effect of polyphenol isolated from the persimmon leaf (diospyros kaki folium) on human skin.many studies have been conducted to find a natural material that has high biologic functions for human skin without any side effects. persimmon leaf has a substantial amount of tannins in different forms; therefore, it was selected as a target material.200516029677
purification and characterisation of a novel chitinase from persimmon (diospyros kaki) with antifungal activity.a novel chitinase from the persimmon fruit was isolated, purified and characterised in this report. the diospyros kaki chitinase (dkc) was found to be a monomer with a molecular mass of 29 kda. it exhibited optimal activity at ph 4.5 with broad ph stability from ph 4.0-9.0. it has an optimal temperature of 60°c and thermostable up to 60°c when incubated for 30 min. the internal peptide sequences of dkc showed similarity with other reported plant chitinases. it has the ability to hydrolyse colloi ...201223411236
molecular identification of 1-cys peroxiredoxin and anthocyanidin/flavonol 3-o-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (diospyros kaki thunb.).fruits of persimmon (diospyros kaki thunb.) accumulate large amounts of proanthocyanidins (pas) in the early stages of development. astringent (a)-type fruits remain rich in soluble pas even after they reach full-mature stage, whereas non-astringent (na)-type fruits lose these compounds before full maturation. as a first step to elucidate the mechanism of pa accumulation in this non-model species, we used suppression subtractive hybridization to identify transcripts accumulating differently in y ...200919641937
acaricidal activity and function of mite indicator using plumbagin and its derivatives isolated from diospyros kaki thunb. roots (ebenaceae).acaricidal effects of materials derived from diospyros kaki roots against dermatophagoides farinae and d. pteronyssinus were assessed using impregnated fabric disk bioassay and compared with that of the commercial benzyl benzoate. the observed responses varied according to dosage and mite species. the ld50 values of the chloroform extract of diospyros kaki roots were 1.66 and 0.96 microg/cm2 against d. farinae and d. pteronyssinus. the chloroform extract of diospyros kaki roots was approximately ...200818309277
neuroprotective effects of a standardized flavonoid extract from diospyros kaki leaves.flavonoids, extracted from the leaves of diospyros kaki, are the main therapeutic components of naoxingqing (nxq), a potent and patented chinese herbal remedy widely used in china for the treatment of apoplexy syndrome.200919665536
protective effect of persimmon (diospyros kaki) peel proanthocyanidin against oxidative damage under h2o2-induced cellular senescence.8-hydroxy-2'-deoxyguanosine (8-ohdg), one of the most abundant oxidative dna adducts, is used as an indicator of oxidative dna damage associated with aging. among homologs of the silent information regulator (sir), sirtuin 1 (sirt1) is suggested as a regulator of the apoptotic response to dna damage. since it has been suggested that the aging process can be delayed by the attenuation of oxidative damage such as dna damage or sirt1 modulation, we focused on the protective effect against cellular ...200818520066
regulation of the peripheral body temperature by foods: a temperature decrease induced by the japanese persimmon (kaki, diospyros kaki).we investigated whether the ingestion of the japanese persimmon (kaki, diospyros kaki) could lower the human peripheral body temperature. it was found that the temperatures recorded at the foot and wrist were depressed after kaki consumption compared to after the same amount of water consumption. the effects of ingesting freeze-dried kaki and eating a cookie (as its nutritional counterpart) containing the same amount of carbohydrate, protein, fat, and water were compared. a similar temperature-r ...200312619669
tree-ring strontium-90 and cesium-137 as potential indicators of radioactive pollution.to examine whether tree rings can be used to detect or assess local historical 90sr or 137cs fallout, such as that resulting from the hiroshima atomic bomb, radial distribution of 90sr and 137cs in trees was examined. we studied a gymnosperm [japanese cedar, cryptomeria japonica (l. f.) d. don] and an angiosperm (japanese persimmon, diospyros kaki thunb.) tree species from the vicinity of the atomic bomb hypocenter, and from other locations in japan. a significant amount of 137cs was detected in ...200212469850
effect of five flavonoid compounds isolated from leaves of diospyros kaki on stimulus-induced superoxide generation and tyrosyl phosphorylation of proteins in human neutrophils.the crude drug "kaki-yô" is a traditional medicine used in japan as a hypotensive drug.200212417109
effect of five triterpenoid compounds isolated from leaves of diospyros kaki on stimulus-induced superoxide generation and tyrosyl phosphorylation in human polymorphonuclear leukocytes.the crude drug "kaki-yô" is a traditional medicine used in japan as a hypotensive drug.200211983195
screening of vasorelaxant activity of some medicinal plants used in oriental medicines.hexane, ethylacetate (etoac), and n-butanol (n-buoh) extracts of medicinal plants traditionally used in the east asia, such as china, korea, and japan were screened for their vasorelaxant activity using isolated rat aorta. among the 60 solvent-extracts from 20 medicinal plants, hexane and n-buoh extracts of diospyros kaki and polygonum aviculare, hexane, etoac, and n-buoh extracts of magnolia liliflora, n-buoh extract of sorbus commixta, and etoac and n-buoh extracts of selaginella tamariscina w ...200515848029
engineered sorbitol accumulation induces dwarfism in japanese persimmon.a cdna encoding sorbitol-6-phosphate dehydrogenase (s6pdh), which is a key enzyme in sorbitol biosynthesis in rosaceae, was introduced into the japanese persimmon (diospyros kaki) to increase the environmental stress tolerance. resultant transformants exhibited salt-tolerance with dwarfing phenotypes. therefore, we studied two transgenic lines to understand the physiological mechanism of this dwarfism: lines ps1 and ps6 accumulated high and moderate levels of sorbitol, respectively. the average ...200415535127
anaphylaxis after ingestion of sharon fruit.the sharon fruit is the edible fruit of the persimmon tree (diospyros kaki) which belongs to the ebenaceae family. there are few references of allergic reaction to this fruit. we introduce a case of a 33-years old man with a anaphylactic reaction immediately after ingestion of sharon fruit.200111420030
inhibition of eukaryotic dna polymerase alpha by persimmon (diospyros kaki) extract and related polyphenols.the effects of persimmon extract (diospyros kaki) and related polyphenols on eukaryotic dna polymerase alpha were examined. it was found that persimmon extract, epigallocatechin gallate, and epicatechin gallate strongly inhibited the activity of dna polymerase alpha purified from calf thymus. among these polyphenols, persimmon extract had the most potent effect on dna polymerase alpha activity and the concentration of persimmon extract producing 50% inhibition of the activity was 0.191 microm. p ...199910365250
transformation of japanese persimmon (diospyros kaki thunb.) with apple cdna encoding nadp-dependent sorbitol-6-phosphate dehydrogenase.japanese persimmon (diospyros kaki thunb. cv jiro) was transformed with apple (malus x domestica borkh.) cdna encoding nadp-dependent sorbitol-6-phosphate dehydrogenase (s6pdh) by an agrobacterium-mediated leaf-disc transformation system. integration and expression of the transgene were confirmed by genomic dna blot and immunoblot analyses. sorbitol accumulation in five of six transgenic plants obtained was confirmed by gc-ms. the amount of sorbitol in the leaves of transgenic plants varied from ...200111297780
inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity.the leaves of the persimmon diospyros kaki, have been traditionally used for treatment of hypertensive diseases in japan. we have studied the inhibitory effects of four flavonoids isolated from the leaves of the persimmon on angiotensin-converting enzyme activity. the four flavonoids astragalin [1], kaempferol-3-o-(2"-o-galloyl)-glucoside [2], isoquercitrin [3], and quercetin-3-o-(2"-o-galloyl)-glucoside [4] inhibited the angiotensin-converting enzyme activity in a dose-dependent fashion. compou ...19873430165
effects and action mechanism of diospyros kaki on the differentiation of human leukemia hl-60 cells.diospyros kaki thunb. (ebenaceae) is widely distributed in north-east asian countries. almost all parts of this plant have been traditionally used as medicine. human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] or all-trans retinoic acid (atra). combination of low doses of atra or 1,25-dihydroxyvitamin d3 that do not induce toxicity with another drug is a useful strategy for acute promyelocytic leukemia therap ...201019956868
24-hydroxyursolic acid from the leaves of the diospyros kaki (persimmon) induces apoptosis by activation of amp-activated protein kinase.there are multiple lines of evidence that persimmon extract and its constituents have potent antitumor activity against human cancer cells. however, the molecular mechanisms of 24-hydroxyursolic acid, a triterpenoid found in persimmon, on antitumor activities are not yet understood. here, we demonstrate that 24-hydroxyursolic acid inhibited cell proliferation, strongly activated amp-activated protein kinase (ampk) and mediated critical anticancer effects by inhibition of cyclooxygenase (cox-2) e ...201019960411
allergy caused by ingestion of persimmon (diospyros kaki): detection of specific ige and cross-reactivity to profilin and carbohydrate determinants.allergy to persimmon (diospyros kaki) is very rare and not yet confirmed by means of double-blind, placebo-controlled, food-challenge (dbpcfc). thus far, specific ige to this fruit and cross-reactivity to pollen and other foods has not been determined.200111295664
Gluconacetobacter kakiaceti sp. nov., a novel acetic acid bacterium isolated from kaki vinegar, Japanese traditional fruit vinegar.Two novel acetic acid bacteria, strains G5-1T and I5-1, were isolated from traditional kaki vinegar, which is produced from fruits of kaki (Diospyros kaki Thunb.), collected in Kumamoto Prefecture, Japan. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strains G5-1T and I5-1 formed a distinct subline in the genus Gluconacetobacter and closely related to Gluconacetobacter swingsii DST GL01T with 99.3 % sequence similarity. Strains G5-1T and I5-1 showed DNA-DNA relatedness ...201121841006
Self-nanoemulsifying drug delivery system of persimmon leaf extract: Optimization and bioavailability studies.In current study, a self-nanoemulsifying drug delivery system (SNEDDS) of persimmon (Diospyros kaki) leaf extract (PLE) was developed and characterized to compare its in vitro dissolution and relative bioavailability with commercially available tablets (Naoxinqing tablets). Pseudo-ternary phase diagrams were constructed by phase diagram by micro plate dilution (PDMPD) method, of which the evaluation method was improved to use Multiskan Ascent for identifying turbidity. The formulation of PLE-loa ...201121884770
major water-soluble polyphenols, proanthocyanidins, in leaves of persimmon (diospyros kaki) and their alpha-amylase inhibitory activity.the amounts and compositions of polyphenol in persimmon leaves and persimmon leaf tea were investigated. the predominant polyphenols in fresh leaves were water-soluble, and the contents reached a maximum (2.40% w/w) in june, and then gradually decreased. separation of them followed by thiolytic degradation revealed that the major components were unique proanthocyanidin oligomers consisting of four heterogeneous extension units, including epigallocatechin-3-o-gallate. persimmon leaf tea also cont ...201020622463
identification of yeast and acetic acid bacteria isolated from the fermentation and acetification of persimmon (diospyros kaki).persimmon (diospyros kaki) is a seasonal fruit with important health benefits. in this study, persimmon use in wine and condiment production was investigated using molecular methods to identify the yeast and acetic acid bacteria (aab) isolated from the alcoholic fermentation and acetification of the fruit. alcoholic fermentation was allowed to occur either spontaneously, or by inoculation with a commercial saccharomyces cerevisiae wine strain, while acetification was always spontaneous; all thes ...201222265289
inhibitory effects of persimmon (diospyros kaki) extract and related polyphenol compounds on growth of human lymphoid leukemia cells.we have investigated the effects of persimmon (diospyros kaki) extract (ps) and related polyphenol compounds such as catechin (c), epicatechin (ec), epicatechingallate (ecg), epigallocatechin (egc), and epigallocatechingallate (egcg) on the growth of human lymphoid leukemia molt 4b cells. we found that ps, ecg, egc, and egcg strongly inhibited the growth of the cells in a dose-dependent manner, while c and ec inhibited the growth of the cells only moderately. ornithine decarboxylase (odc), a rat ...19979255971
induction of uncoupling protein-1 and -3 in brown adipose tissue by kaki-tannin in type 2 diabetic nsy/hos mice.kaki-tannin, a highly polymerized-tannin from the young fruits of persimmon (diospyros kaki 'hachiya'), has been shown to have bile acid-binding activity. to verify the effect of kaki-tannin on the metabolism of lipid and glucose in type 2 diabetes, type 2 diabetic nsy/hos mice were fed an ain76-modified high fat diet supplemented with 1% (w/w) kaki-tannin for 8weeks. kaki-tannin induced a 2-fold increase in fecal bile acid excretion and was significantly effective in the prevention of a rise in ...201122079182
evaluation to the antioxidant activity of total flavonoids extract from persimmon (diospyros kaki l.) leaves.persimmon leaves are commonly consumed as beverages, but are also used as a popular folk medicine in china. the purpose of this work is to assess the antioxidant activity of an extract of total flavonoids from persimmon leaves (tfpl). the effect of tfpl on total antioxidant activity, reducing power, 1,1-diphenyl-2-picrylhydrazyl (dpph()) radical scavenging, superoxide anion (()o(2)(-)) radical scavenging, hydroxyl (oh()) radical scavenging and metal chelating activities was examined. we found th ...201121802475
bile acid-binding ability of kaki-tannin from young fruits of persimmon (diospyros kaki) in vitro and in vivo.the bile acid-binding ability of a highly polymerized tannin (kaki-tannin) extracted from dried-young fruits of persimmon (diospyros kaki) was examined. the kaki-tannin was composed mainly of epicatechin, epigallocatechin, epicatechin-3-o-gallate and epigallocatechin-3-o-gallate. bile acid-binding ability of kaki-tannin was examined against cholic acid, glycocholic acid, taurocholic acid and deoxycholic acid in vitro, and its effect on fecal bile acid excretion in mice was also examined. althoug ...201020922818
plant antimutagens and their mixtures in inhibition of genotoxic effects of xenobiotics and aging processes.the antimutagenic effect of the bioactive compounds from fruits of morus alba l. (ma), punica granatum l. (pg), diospyros kaki l. (dk), cydonia oblonga mill. (co) and roots of glycyrrhiza glabra (gg) were investigated. the antimutagenic effects of compounds separately as well as their action in complex mixtures were studied on mutations induced by genotoxicants (x-rays, n-methylnitrosourea, cyclophosphamide, naf) and aging in bone marrow cell chromosomes from mice and rats. when tested separatel ...200212570329
dkmyb4 is a myb transcription factor involved in proanthocyanidin biosynthesis in persimmon fruit.proanthocyanidins (pas) are secondary metabolites that contribute to the protection of the plant and also to the taste of the fruit, mainly through astringency. persimmon (diospyros kaki) is unique in being able to accumulate abundant pas in the fruit flesh. fruits of the nonastringent (na)-type mutants lose their ability to produce pa at an early stage of fruit development, while those of the normal astringent (a) type remain rich in pa until fully ripened. the expression of many pa pathway gen ...200919783643
ethylene biosynthesis in detached young persimmon fruit is initiated in calyx and modulated by water loss from the fruit.persimmon (diospyros kaki thunb.) fruit are usually classified as climacteric fruit; however, unlike typical climacteric fruits, persimmon fruit exhibit a unique characteristic in that the younger the stage of fruit detached, the greater the level of ethylene produced. to investigate ethylene induction mechanisms in detached young persimmon fruit, we cloned three cdnas encoding 1-aminocyclopropane-1-carboxylic acid (acc) synthase (dk-acs1, 2, and -3) and two encoding acc oxidase (dk-aco1 and -2) ...012529535
selection of suitable reference genes for rt-qpcr normalization under abiotic stresses and hormone stimulation in persimmon (diospyros kaki thunb).the success of quantitative real-time reverse transcription polymerase chain reaction (rt-qpcr) to quantify gene expression depends on the stability of the reference genes used for data normalization. to date, systematic screening for reference genes in persimmon (diospyros kaki thunb) has never been reported. in this study, 13 candidate reference genes were cloned from 'nantongxiaofangshi' using information available in the transcriptome database. their expression stability was assessed by geno ...201627513755
hepatic gene expression of the insulin signaling pathway is altered by administration of persimmon peel extract: a dna microarray study using type 2 diabetic goto-kakizaki rats.persimmon (diospyros kaki) is a very popular fruit in east asian countries, but its peels are not consumed despite the fact that they contain many antioxidants such as carotenoids and polyphenols. we prepared a fat-soluble extract from persimmon peel (pp) and fed type 2 diabetic goto-kakizaki (gk) rats an ain-93g rodent diet supplemented with persimmon peel extract (pp diet) for 12 weeks. compared with the control ain-93g diet, the pp diet significantly reduced plasma glutamic-pyruvate transamin ...201121370910
evaluation of particle size influence on proximate composition, physicochemical, techno-functional and physio-functional properties of flours obtained from persimmon (diospyros kaki trumb.) coproducts.the aim of the work was to study the influence of particle size in the composition, physicochemical, techno-functional and physio-functional properties of two flours obtained from persimmon (diospyros kaki trumb. cvs. 'rojo brillante' (rbf) and 'triump' (thf) coproducts. the cultivar (rbf and thf) and particle size significantly affected all parameters under study, although depending on the evaluated property, only one of these effects predominated. carbohydrates (38.07-46.98 g/100 g) and total ...201728093647
the leaf of diospyros kaki thumb ameliorates renal oxidative damage in mice with type 2 diabetes.diabetic kidney disease is the most common and severe chronic complication of diabetes. the leaf of diospyros kaki thumb (persimmon) has been commonly used for herbal tea and medicinal purposes to treat a variety of conditions, including hypertension and atherosclerosis. however, the effect of persimmon leaf on kidney failure has not been investigated. this study aimed to examine the role of persimmon leaf in protecting the diabetes-associated kidney damage in a mouse model of type 2 diabetes. m ...201628078262
epigenetic regulation of the sex determination gene megi in polyploid persimmon.epigenetic regulation can add a flexible layer to genetic variation, potentially enabling long-term but reversible cis-regulatory changes to an allele while maintaining its dna sequence. here, we present a case in which alternative epigenetic states lead to reversible sex determination in the hexaploid persimmon diospyros kaki previously, we elucidated the molecular mechanism of sex determination in diploid persimmon and demonstrated the action of a y-encoded sex determinant pseudogene called og ...201627956470
restorative role of persimmon leaf (diospyros kaki) to gamma irradiation-induced oxidative stress and tissue injury in rats.to evaluate the efficacy of persimmon leaf extract (pl (as a rich plant source) in modulation of radiation-induced liver injury and some metabolic variations in gamma-irradiated rats.201727834112
molecular cloning and functional characterization of dkmate1 involved in proanthocyanidin precursor transport in persimmon (diospyros kaki thunb.) fruit.persimmon fruits accumulate a large amount of proanthocyanidins (pas) in "tannin cells" during development that cause the sensation of astringency due to coagulation of oral proteins. pollination-constant non-astringent (pcna) is a spontaneous mutant persimmon phenotype that loses its astringency naturally on the tree at maturity; while the more common non-pcna fruits remain rich in pas until they are fully ripened. here, we isolated a dkmate1 gene encoding a multidrug and toxic compound extrusi ...201627472890
persimmon breeding in japan for pollination-constant non-astringent (pcna) type with marker-assisted selection.oriental persimmon (diospyros kaki) originated in eastern asia, and many indigenous cultivars have been developed in china, japan, and korea. these cultivars are classified into four groups based on their natural astringency loss on the tree and seed formation: pollination-constant non-astringent (pcna), pollination-variant non-astringent (pvna), pollination-constant astringent (pca), and pollination-variant astringent (pva). pcna is the most desirable type because the fruit can be eaten without ...201627069391
comparison of sensory and compositions of five selected persimmon cultivars (diospyros kaki l.) and correlations between chemical components and processing characteristics.persimmon is one of the most popular and valuable fruits in markets because of its distinguished flavor as well as its significant benefits to health. the present study systematically compared the sensory characteristics and chemical compositions of five commercial persimmon fruits, and the correlations between chemical components and processing characteristics were also included. our results showed that obvious differences in aroma and taste were found among the cultivars. likewise, the content ...201627570285
anti-inflammatory potential of quercetin-3-o-β-d-("2"-galloyl)-glucopyranoside and quercetin isolated from diospyros kaki calyx via suppression of map signaling molecules in lps-induced raw 264.7 macrophages.diospyros kaki (dk) contains an abundance of flavonoids and has been used in folk medicine in korea for centuries. here, we report for the first time the anti-inflammatory activities of quercetin (qct) and quercetin 3-o-β-("2"-galloyl)-glucopyranoside (q32g) isolated from dk. we have determine the no cytotoxicity of q32g and qct against raw 264.7 cells up to concentration of 50 μm. qct and q32g demonstrated potent anti-inflammatory activities by reducing expression of nitric oxide (no), tumor ne ...201627648736
anti-inflammatory effects and mechanisms of action of coussaric and betulinic acids isolated from diospyros kaki in lipopolysaccharide-stimulated raw 264.7 macrophages.diospyros kaki thunb. is widely distributed in east asian countries, its leaves being mainly used for making tea. in this study, coussaric acid (ca) and betulinic acid (ba), both triterpenoid compounds, were obtained from d. kaki leaf extracts through bioassay-guided isolation. ca and ba showed anti-inflammatory effects via inhibition of the nuclear factor-κb (nf-κb) pathway, providing important information on their anti-inflammatory mechanism. furthermore, they markedly inhibited nitric oxide ( ...201627618005
evaluation of the formation of volatiles and sensory characteristics of persimmon (diospyros kaki l.f.) fruit wines using different commercial yeast strains of saccharomyces cerevisiae.this study evaluated the effects of five strains (iffi 1346, iffi 1363, cicc 31482, d254 and cgmcc2.346) of the yeast saccharomyces cerevisiae on the aromatic profiles of fermented persimmon (diospyros kaki l.f.) musts. a total of 50 and 60 compounds were identified in persimmon wine by stir bar sorptive extraction coupled with gas chromatography-mass spectrometry. according to odour activity values (oavs), 26 detected compounds showed an oav above 1. principal component analysis explained the d ...201425186058
dkpk genes promote natural deastringency in c-pcna persimmon by up-regulating dkpdc and dkadh expression.the astringency of chinese pollination-constant non-astringent (c-pcna) persimmon (diospyros kaki thunb.) can be naturally removed on the tree. this process is controlled by a single locus and is dominant against other types of persimmons; therefore, this variant is an important candidate for commercial cultivation and the breeding of pcna cultivars. in our previous study, six full-length coding sequences (cds) for pyruvate kinase genes (dkpk1-6) were isolated, and dkpk1 is thought to be involve ...201728243247
extraction, identification and photo-physical characterization of persimmon (diospyros kaki l.) carotenoids.carotenoid pigments were extracted and purified from persimmon fruits using accelerated solvent extraction (ase). eleven pigments were isolated and five of them were clearly identified as all-trans-violaxanthine, all-trans-lutein, all-trans-zeaxanthin all-trans-cryptoxanthin and all-trans-β-carotene. absorption and fluorescence spectra were recorded. to evaluate the potential of ¹o₂ quenching of the purified carotenoids, we used a monocarboxylic porphyrin (p1cooh) as the photosensitizer to produ ...201728231085
data in support of antioxidant activities of the non-extractable fraction of dried persimmon (diospyros kaki thunb.).this data article is related to the research article entitled, "antioxidant potential in non-extractable fraction of dried persimmon (diospyros kaki thunb.)" (matsumura et al., 2016) [1]. we investigated antioxidant activities of the non-extractable fraction of dried persimmon fruits in vitro and in vivo. we evaluated both extracted fraction and non-extractable fraction, and reported that non-extractable fraction may possess significantly antioxidant potential in vivo on the basis of the oxygen ...201627547805
optical inspection and morphological analysis of diospyros kaki plant leaves for the detection of circular leaf spot disease.the feasibility of using the bio-photonic imaging technique to assess symptoms of circular leaf spot (cls) disease in diospyros kaki (persimmon) leaf samples was investigated. leaf samples were selected from persimmon plantations and were categorized into three groups: healthy leaf samples, infected leaf samples, and healthy-looking leaf samples from infected trees. visually non-identifiable reduction of the palisade parenchyma cell layer thickness is the main initial symptom, which occurs at th ...201627529250
evaluation studies of persimmon plant (diospyros kaki) for physiological benefits and bioaccessibility of antioxidants by in vitro simulated gastrointestinal digestion.this study aims to analyze the antioxidant benefits from persimmon leaf tea, fruit and fibres taking into account their changes along gastrointestinal digestion. the evolution of polyphenols, flavonoids and antioxidant capacity was studied using the recent harmonized in vitro protocol published by minekus et al. (2014). the digestion was performed with and without digestive enzymes. results showed aqueous leaf extract was richer in antioxidants than the fruit or the extracted fibres. nevertheles ...201727507501
five complete chloroplast genome sequences from diospyros: genome organization and comparative analysis.diospyros is the largest genus in ebenaceae, comprising more than 500 species with remarkable economic value, especially diospyros kaki thunb., which has traditionally been an important food resource in china, korea, and japan. complete chloroplast (cp) genomes from d. kaki, d. lotus l., d. oleifera cheng., d. glaucifolia metc., and diospyros 'jinzaoshi' were sequenced using illumina sequencing technology. this is the first cp genome reported in ebenaceae. the cp genome sequences of diospyros ra ...201627442423
co-production of tannase and pectinase by free and immobilized cells of the yeast rhodotorula glutinis mp-10 isolated from tannin-rich persimmon (diospyros kaki l.) fruits.hyper tannase and pectinase-producing yeast rhodotorula glutinis mp-10 was isolated from persimmon (diospyros kaki l.) fruits. the main pectinase activity of yeast was exo-polygalacturonase. no pectin methyl esterase and too low pectin lyase activities were detected for this yeast. the maximum exo-activities of tannase and polygalacturonase were determined as 15.2 and 26.9 u/ml for free cells and 19.8 and 28.6 u/ml for immobilized cells, respectively. immobilized cells could be reused in 13 succ ...201322717667
topical application of a cleanser containing extracts of diospyros kaki folium, polygonum cuspidatum and castanea crenata var. dulcis reduces skin oil content and pore size in human skin.the effects of skin pores on skin topographic features can be reduced by decreasing excessive production and accumulation of sebum and elimination of comedones. therefore, a cosmetic cleanser that regulates sebum homeostasis is required. in the present study, the effects of a cosmetic cleanser that contained diospyros kaki folium, polygonum cuspidatum and castanea crenata var. dulcis (dpc) was examined on the removal of sebum and on skin pore size. healthy volunteers (n=23) aged 20-50 years were ...201526137233
proanthocyanidins in an astringent persimmon inhibit salmonella pathogenicity island 1 (spi1) secretion.astringent compounds contained in persimmon fruits have been widely used in japan as food preservatives and thus as anti-bacterial and anti-fungi reagents. however, the molecular mechanism of the anti-microbial activity has been unclear. one of the virulence secretion systems in salmonella enterica was used to test the anti-microbial activity of extracts from a persimmon (diospyros kaki thunb 'saijo').201626041125
transferability of retrotransposon primers derived from persimmon (diospyros kaki thunb.) across other plant species.retrotransposon-based molecular markers are powerful molecular tools. however, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-consuming and costly. therefore, research into the transferability of retrotransposon primers developed from one plant species onto others would ...201323913371
inactivation of pathogenic viruses by plant-derived tannins: strong effects of extracts from persimmon (diospyros kaki) on a broad range of viruses.tannins, plant-derived polyphenols and other related compounds, have been utilized for a long time in many fields such as the food industry and manufacturing. in this study, we investigated the anti-viral effects of tannins on 12 different viruses including both enveloped viruses (influenza virus h3n2, h5n3, herpes simplex virus-1, vesicular stomatitis virus, sendai virus and newcastle disease virus) and non-enveloped viruses (poliovirus, coxsachievirus, adenovirus, rotavirus, feline calicivirus ...201323372851
study of antiobesity effect through inhibition of pancreatic lipase activity of diospyros kaki fruit and citrus unshiu peel.pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. the inhibitory activity on pancreatic lipase enzyme of diospyros kaki fruit and citrus unshiu peel mixture extract (pcm) w ...201627529064
physicochemical characterization of pure persimmon juice: nutritional quality and food acceptability.technological process for production of non-astringent persimmon (diospyros kaki thunb. cv. "rojo brillante") juice was described. the degree of fruit ripening expressed as color index (ci) varied between 12.37 and 16.33. persimmon juice was characterized by determining physicochemical quality parameters as yield, total soluble solids (tss), ph, titratable acidity (ta), organic acids, and main sugars. a thermal treatment of 90 ºc for 10 s was effective in controlling naturally occurring microorg ...201525619747
identification and characterization of micrornas from chinese pollination constant non-astringent persimmon using high-throughput sequencing.micrornas (mirnas) have been shown to play key roles in regulating gene expression at post-transcriptional level, but mirnas associated with natural deastringency of chinese pollination-constant nonastringent persimmon (cpcna) have never been identified.201525604351
dkmyb2 wound-induced transcription factor of persimmon (diospyros kaki thunb.), contributes to proanthocyanidin regulation.proanthocyanidins (pas) are secondary metabolites that contribute to the protection of a plant against biotic and abiotic stresses. persimmon (diospyros kaki) accumulates abundant pas in each plant organ, and some potential myb-like transcription factors (myb-tfs) involved in the production of pas have been isolated. in this study, we aimed to molecularly characterize one of them, dkmyb2, which was placed in a subclade including a pa regulator of arabidopsis (arabidopsis thaliana), transparent t ...201020690029
extraction, purification and anti-radiation activity of persimmon tannin from diospyros kaki l.f.in this study, persimmon tannin was extracted from diospyros kaki l.f. using ultrasound-assisted extraction and purified by d101 macroporous resin column chromatography and polysulfone ultrafiltration membrane. the tannin content of the final persimmon tannin extracts was attained to 39.56% calculated as catechin equivalents. also, the radioprotective effects of persimmon tannin for hek 293t cells proliferation and apoptosis after gamma irradiation were investigated by cck-8, hoechst 33258 stain ...201627267156
three new species of mealybug (hemiptera, coccomorpha, pseudococcidae) on persimmon fruit trees (diospyros kaki) in southern brazil.brazil has the greatest insect diversity in the world; however, little is known about its scale insect species (hemiptera: coccomorpha). mealybugs (pseudococcidae) have been found in at least 50% of persimmon orchards diospyros kaki l. in the southern part of the country. in this study three new mealybug species on persimmon trees located in the serra gaúcha region, rs, brazil, namely, anisococcus granarae pacheco da silva & kaydan, sp. n., ferrisia kaki kaydan & pacheco da silva, sp. n. and pse ...201627199595
response surface methodology applied to supercritical fluid extraction (sfe) of carotenoids from persimmon (diospyros kaki l.).supercritical carbon dioxide with ethanol as co-solvent was used to extract carotenoids from persimmon fruits (diospyros kaki l.). based on a response surface methodology (rsm), a predicting model describing the effects of co2 temperature, pressure, flow rate, ethanol percentage and extraction time was set up for each of the four carotenoids of interest. the best extraction yields in our experimental domain were found at 300 bars, 60°c, 25% (w/w) ethanol, 3ml/min flow rate and 30min for xanthoph ...201627132842
the leaves of diospyros kaki exert beneficial effects on a benzalkonium chloride-induced murine dry eye model.in this study, the beneficial effects of the oral administration of ethanol extract of diospyros kaki (eedk) were tested on a mouse dry eye model induced by benzalkonium chloride (bac).201627110091
protective effect of diospyros kaki against glucose-oxygen-serum deprivation-induced pc12 cells injury.ischemic cerebrovascular disease is one of the most common causes of death in the world. recent interests have been focused on natural antioxidants and anti-inflammatory agents as potentially useful neuroprotective agents. diospyros kaki (persimmon) has been shown to exert anti-inflammatory, antioxidant, and antineoplastic effects. however, its effects on ischemic damage have not been evaluated. here, we used an in vitro model of cerebral ischemia and studied the effects of hydroalcoholic extrac ...201626941791
isolation and characterization of dkpk genes associated with natural deastringency in c-pcna persimmon.chinese pollination-constant non-astringent (c-pcna) persimmon (diospyros kaki thunb.) is considered to be an important germplasm resource for the breeding of pcna cultivars, though its molecular mechanisms of astringency removal remain to be elucidated. previously, we showed that the abundance of pyruvate kinase gene transcripts increased rapidly during astringency removal in c-pcna persimmon fruit. here, we report the full-length coding sequences of six novel dkpk genes from c-pcna persimmon f ...201626925075
antioxidant potential in non-extractable fractions of dried persimmon (diospyros kaki thunb.).dried fruits of persimmon (diospyros kaki thunb.) are a traditional food in japan and contain large quantities of tannins. in this study, we investigated the in vitro and in vivo antioxidant potentials of non-extractable fractions from dried persimmons. hydrolysed non-extractable fractions showed the highest antioxidant activities in vitro. in subsequent experiments, the plasma oxygen radical absorbance capacity (orac) values in rats supplemented with a 5% non-extractable fraction were approxima ...201626920271
the persimmon 9-lipoxygenase gene dklox3 plays positive roles in both promoting senescence and enhancing tolerance to abiotic stress.the lipoxygenase (lox) pathway is a key regulator for lipid peroxidation, which is crucial for plant senescence and defense pathways. in this study, the transcriptional expression patterns of three persimmon (diospyros kaki l. 'fupingjianshi') 9-lipoxygenase genes (dklox1, dklox3, and dklox4) were investigated. dklox1 was specifically expressed in fruit, particularly in young fruit, and showed little response to the postharvest environments. dklox4 was expressed in all tissues and slightly stimu ...201526697033
pectin methylesterase from kiwi and kaki fruits: purification, characterization, and role of ph in the enzyme regulation and interaction with the kiwi proteinaceous inhibitor.pectin methylesterase was purified from kiwi (actinidia chinensis) and kaki fruit (diospyros kaki). the ph values of the fruit homogenates were 3.5 and 6.2, respectively. the kiwi enzyme is localized in the cell wall and has a neutral-alkaline pi, whereas the kaki enzyme is localized in the soluble fraction and has a neutral-acidic pi. the molecular weights of the kiwi and kaki enzymes were 50 and 37 kda, respectively. the two enzymes showed a similar salt and ph dependence of activity, and a di ...200415675822
acidic electrolyzed water efficiently improves the flavour of persimmon (diospyros kaki l. cv. mopan) wine.the ability of acidic (acw) and alkaline electrolyzed waters (alw) to improve the flavour of persimmon (diospyros kaki l.) wine was evaluated. wines made with acw (wacw) were significantly better than wines made with alw or pure water (pw) in aroma, taste, and colour. volatile analysis showed that wacw has high alcohol and ester contents, including 2-phenylethanol, isopentanol, isobutanol, ethyl dodecanoate, phenethyl acetate, and butanedioic acid diethyl ester. the total amino acid content of p ...201626616934
quercetin-3-o-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranoside suppresses melanin synthesis by augmenting p38 mapk and creb signaling pathways and subsequent camp down-regulation in murine melanoma cells.in this study, the effect of purified quercetin-3-o-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosid (qcgg) on melanogenesis was investigated. qcgg was isolated from the calyx of a traditional korean medicinal herb, persimmon (diospyros kaki). the hypopigmentation effects of qcgg were determined by examination of cellular melanin contents, tyrosinase activity assay, camp assay, and western blotting of α-msh-stimulated b16f10 mouse melanoma cells. our results showed that qcgg inhibited both melanin ...201526586997
nmr-based analysis of the chemical composition of japanese persimmon aqueous extracts.japanese persimmon (diospyros kaki l.) is recognized as an outstanding source of biologically active compounds relating to many health benefits. in the present study, nmr spectroscopy provided a comprehensive metabolic overview of japanese persimmon juice. detailed signal assignments of japanese persimmon juice were carried out using various 2d nmr techniques incorporated with broadband water suppression enhanced through t1 effects (bb-wet) or wet sequences, and 26 components, including minor co ...201626482562
selected chemical compounds in firm and mellow persimmon fruit before and after the drying process.persimmon is a seasonal fruit and only available in fresh form for a short period of each year. in addition to freezing, drying is the simplest substitute for the fresh fruit and accessible throughout the year. the effect of mellowing and drying was evaluated in 'tipo' persimmon, an astringent cultivar.201626455574
diospyros kaki extract inhibits alkali burn-induced corneal neovascularization.the purpose of this study was to evaluate the effect of ethanol extract of diospyros kaki (eedk) leaves on corneal neovascularization (conv) in rats. one week after the alkali burns in the corneas, the conv area coverage in the conv-positive control group, 100 mg/kg eedk group, and 200 mg/kg eedk group was 43.3% ± 5.5%, 337.7% ± 2.5%, and 27.2% ± 4.3%, respectively. the areas of conv in the eedk-treated groups were significantly different from those of the conv group. eedk significantly attenuat ...201626348484
investigation and analysis of genetic diversity of diospyros germplasms using scot molecular markers in guangxi.knowledge about genetic diversity and relationships among germplasms could be an invaluable aid in diospyros improvement strategies.201526317414
leaves of persimmon (diospyros kaki thunb.) ameliorate n-methyl-n-nitrosourea (mnu)-induced retinal degeneration in mice.the purpose of the study was to investigate the protective effects of the ethanol extract of diospyros kaki (eedk) persimmon leaves to study n-methyl-n-nitrosourea (mnu)-induced retinal degeneration in mice. eedk was orally administered after mnu injection. retinal layer thicknesses were significantly increased in the eedk-treated group compared with the mnu-treated group. the outer nuclear layer was preserved in the retinas of eedk-treated mice. moreover, eedk treatment reduced the mnu-dependen ...201526260943
immunostimulatory polysaccharide isolated from the leaves of diospyros kaki thumb modulate macrophage via tlr2.the objective of this study was to investigate the immunostimulatory effects of a polysaccharide fraction from the leaves of diospyros kaki thumb (ple0) and the molecular mechanism responsible for its action in raw 264.7 macrophages as well as in vivo effects on cyclophosphamide-induced immunosuppression in mice. ple0 concentration-dependently activated the expressions of inducible nitric oxide synthase (inos) at the protein and mrna levels and its promoter activity, and that these activations c ...201526093315
accelerated solvent extraction of carotenoids from: tunisian kaki (diospyros kaki l.), peach (prunus persica l.) and apricot (prunus armeniaca l.).extraction of carotenoids from biological matrices and quantifications remains a difficult task. accelerated solvent extraction was used as an efficient extraction process for carotenoids extraction from three fruits cultivated in tunisia: kaki (diospyros kaki l.), peach (prunus persica l.) and apricot (prunus armeniaca l.). based on a design of experiment (doe) approach, and using a binary solvent consisting of methanol and tetrahydrofuran, we could identify the best extraction conditions as be ...201525872435
analysis of xyloglucan endotransglycosylase/hydrolase (xth) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening.xyloglucan endotransglycosylase/hydrolase (xth) enzymes have played a role in the remodeling of cell wall hemicelluloses. to investigate the function of xths in persimmon (diospyros kaki l.) fruit development and postharvest softening, five cdnas (dkxth1 to dkxth5), whose putative proteins contained the conserved deidfeflg motif of xth, were cloned. real time quantitative pcr analysis revealed that dkxth1, dkxth4, and dkxth5 peaked in immature expanding fruit, and their higher expression was obs ...201525849978
occurrence of sooty blotch and flyspeck disease on sweet persimmon in korea.sooty blotch and flyspeck (sbfs), a disease caused by a complex of fungi, results in substantial economic losses for commercial growers of sweet persimmon (diospyros kaki l.) in korea. however, many species causing sbfs in korea have not been identified and sources of inoculum are uncertain. based on mycological characteristics, pathogenicity, and molecular data, the causal fungi were identified as dissoconium sp. and zygophiala wisconsinensis. this is the first report of sbfs of sweet persimmon ...201223115517
persimmon (diospyros kaki l.) leaves: a review on traditional uses, phytochemistry and pharmacological properties.persimmon (diospyros kaki l.) leaves, known as shi ye (in chinese), have a long history as a chinese traditional medicine for the treatment of ischemia stroke, angina, internal hemorrhage, hypertension, atherosclerosis and some infectious diseases, etc. additionally, persimmon leaves could be used as healthy products, cosmetics and so on, which have become increasingly popular in asia, such as japan, korea and china etc.201525637828
effects of phospholipid complexes of total flavonoids from persimmon (diospyros kaki l.) leaves on experimental atherosclerosis rats.the total flavonoids from persimmon leaves (plf), extracted from the leaves of diospyros kaki l. dispryosl and ebenaceae, is reported to possess many beneficial health effects. however, the oral bioavailability of plf is relatively low due to its poor solubility. in the present study, the phospholipid complexes of total flavonoids from persimmon leaves (plf-pc) was prepared to enhance the oral bioavailability of plf and to evaluate its antiatherosclerotic properties in atherosclerosis rats in co ...201627340105
a comparative study on the in vitro antioxidant potentials of three edible fruits: cornelian cherry, japanese persimmon and cherry laurel.this study was designed in order to investigate in vitro antioxidant potentials of 80% methanolic extracts prepared from three edible fruits, cornus mas l., diospyros kaki l., laurocerasus officinalis roem. for this purpose, 8 different tests were performed including 2,2-diphenyl-1-picrylhydrazyl (dpph) and superoxide radical scavenging tests, ferric reducing antioxidant power (frap), cupric reducing antioxidant capacity (cuprac), metal-chelating capacity, determination of total antioxidant capa ...201222713712
discovery and molecular characterization of a new cryptovirus dsrna genome from japanese persimmon through conventional cloning and high-throughput sequencing.through the application of next generation sequencing, in synergy with conventional cloning of dop-pcr fragments, two double-stranded rna (dsrna) molecules of about 1.5 kbp in size were isolated from leaf tissue of a japanese persimmon (accession sspi) from apulia (southern italy) showing veinlets necrosis. high-throughput sequencing allowed whole genome sequence assembly, yielding a 1,577 and a 1,491 bp contigs identified as dsrna-1 and dsrna-2 of a previously undescribed virus, provisionally n ...201525315633
rapid screening of low-molecular-weight phenols from persimmon (diospyros kaki) pulp using liquid chromatography/uv-visible/electrospray mass spectrometry analysis.persimmon fruits have been widely used in traditional medicine owing to their phenolic composition. this research aims to perform a rapid, detailed and affordable study of the profile of low-molecular-weight phenols from persimmon pulp.201525103690
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