| clinical importance of bilophila wadsworthia. | bilophila wadsworthia is an anaerobic, gram-negative, asaccharolytic, urease-positive, bile-resistant, catalase-positive bacillus, originally recovered from infections in patients with gangrenous and perforated appendicitis. additional isolations from clinical specimens, including pleural fluid, joint fluid, blood and pus from a scrotal abscess, mandibular osteomyelitis and axillary hidradenitis suppurativa are described here. bilophila is found as normal flora in feces and, occasionally, in sal ... | 1992 | 1295759 |
| bilophila wadsworthia bacteremia in two patients with hepatic abscesses. | bilophila wadsworthia, a recently described anaerobic gram-negative bacillus, was isolated from blood cultures of two patients with liver abscesses. these isolates exhibited the expected phenotypic characteristics, and identification was confirmed by cell wall fatty acid analysis by gas-liquid chromatography. this is the first documentation of bacteremia due to b. wadsworthia. | 1992 | 1401025 |
| antimicrobial susceptibility testing of bilophila wadsworthia by using triphenyltetrazolium chloride to facilitate endpoint determination. | initial susceptibility studies of bilophila wadsworthia indicated significant resistance to several beta-lactam antibiotics, including imipenem and cefoxitin. no beta-lactamase production was detected. however, some b. wadsworthia strains may grow as a heavy "haze" at up to the highest concentration of an antibiotic on standard antimicrobial agent-containing plates, and it is often difficult to determine the point at which conventional growth stops and haze growth begins. we investigated the nat ... | 1992 | 1416848 |
| in-vitro activity of twelve antibiotics against clinical isolates of bilophila wadsworthia. | | 1992 | 1607338 |
| bilophila wadsworthia isolates from clinical specimens. | bilophila wadsworthia is an anaerobic, gram-negative, asaccharolytic, bile-resistant, catalase-positive bacillus that is usually urease positive and was originally recognized in specimens of peritoneal fluid and tissue from patients with appendicitis. additional isolations from clinical specimens, including a scrotal abscess, mandibular osteomyelitis, axillary hidradenitis suppurativa, pleural fluid, joint fluid, and blood, are described here. | 1992 | 1629348 |
| amplification of bacterial 16s ribosomal dna with polymerase chain reaction. | the sequence of small-subunit rrna varies in an orderly manner across phylogenetic lines and contains segments that are conserved at the species, genus, or kingdom level. by directing oligonucleotide primers at sequences conserved throughout the eubacterial kingdom, we amplified bacterial 16s ribosomal dna sequences with the polymerase chain reaction. priming sites were located at the extreme 5' end, the extreme 3' end, and the center of 16s ribosomal dna. the isolates tested with these primers ... | 1990 | 2095137 |
| bilophila wadsworthia, gen. nov. and sp. nov., a unique gram-negative anaerobic rod recovered from appendicitis specimens and human faeces. | strongly catalase-positive gram-negative anaerobic rods were isolated from approximately half of all intra-abdominal specimens received from patients with gangrenous and perforated appendicitis, and subsequently also from normal faecal specimens. the organism was originally detected on bacteroides-bile-aesculin (bbe) agar, and grew slowly on non-selective anaerobic media containing blood. it was stimulated by bile and differed from other known genera by being urease- and catalase-positive, and b ... | 1989 | 2636263 |
| [isolation of bilophila wadsworthia from peritoneal fluid of a patient with an infected douglas pouch]. | | 1995 | 7499920 |
| preliminary study using species-specific oligonucleotide probe for rrna of bilophila wadsworthia. | portions of the 16s rna from a urease-positive bilophila wadsworthia strain were sequenced, and a probe was constructed. the probe was end labeled with [32p]atp and polynucleotide kinase and hybridized on a nylon filter (by dot blot hybridization) to the immobilized rrna of 12 b. wadsworthia strains and eight other anaerobic isolates. the probe efficiently hybridized only to the bilophila strains. cross-reactivity at high rna levels (2,000 ng) was observed with one strain of bacteroides thetaiot ... | 1994 | 7529241 |
| effect of iron depletion on protein profiles of bilophila wadsworthia. | | 1995 | 7548540 |
| bilophila wadsworthia isolates from clinical specimens. | | 1995 | 7548555 |
| inactivation of anaerobic bacteria by various photosensitized porphyrins or by hemin. | the photodynamic effects of deuteroporphyrin (dp), hematoporphyrin derivative (hpd), hematoporphyrin (hp), or protoporphyrin (pp) on a variety of anaerobic microorganisms were examined in this study. the majority of the species, among the 350 strains tested, were inhibited by concentrations of < or = 2.5 micrograms/ml of light-activated dp. species found to be resistant to this treatment included bilophila wadsworthia, fusobacterium mortiferum, fusobacterium varium, and bacteroides gracilis. the ... | 1994 | 7765091 |
| in vitro activity of du-6859a against anaerobic bacteria. | the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ... | 1994 | 7840599 |
| bilophila wadsworthia bacteremia in a patient with gangrenous appendicitis. | | 1994 | 8086539 |
| morphological response of bilophila wadsworthia to imipenem: correlation with properties of penicillin-binding proteins. | the penicillin-binding protein (pbp) patterns of six strains of bilophila wadsworthia were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and subsequent fluorography of membrane preparations labelled with [3h]benzylpenicillin. the pbp profiles among the strains were similar; generally, seven to nine pbp-reactive bands could be visualized; their molecular weights ranged from 31 to 137 kda. the relative affinities of the pbps of four strains of b. wadsworthia fo ... | 1993 | 8109929 |
| comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. | cellular fatty acid profiles were determined for species in 33 genera of anaerobic gram-negative bacilli and were confirmed to be a useful taxonomic tool. most of the genera could be differentiated by visual inspection of their profiles. the three genus pairs that were most difficult to distinguish visually (bacteroides and prevotella, pectinatus and megamonas, and serpulina and bilophila) and the species of these genera were differentiated by the midi (microbial id, inc.) identification system. ... | 1994 | 8186100 |
| in vitro activity of bay y3118 against anaerobic bacteria. | the antimicrobial activity of a new quinolone, bay y3118, was determined against 326 strains of anaerobic bacteria and compared with the activities of ampicillin-sulbactam, cefotetan, clindamycin, imipenem, metronidazole, and sparfloxacin. the national committee for clinical laboratory standards-approved wadsworth agar dilution technique with brucella-laked blood agar was used throughout the study. breakpoints used to determine the percent susceptible were 2 micrograms/ml for bay y3118 and sparf ... | 1993 | 8285647 |
| recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms. | the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ... | 1993 | 8324113 |
| bactericidal activity of selected antimicrobial agents against bilophila wadsworthia and bacteroides gracilis. | bactericidal assays of bacteroides gracilis (six strains) and bilophila wadsworthia (12 strains) in brucella broth with appropriate supplements were performed by the time-kill kinetic method. antimicrobial agents tested were ampicillin/sulbactam (final concentrations, 16/8 micrograms/ml), ticarcillin/clavulanate (128/2 micrograms/ml), imipenem (8 micrograms/ml), cefoxitin (32 micrograms/ml), chloramphenicol (16 micrograms/ml), clindamycin (4 micrograms/ml), and metronidazole (16 micrograms/ml). ... | 1993 | 8324144 |
| in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria. | the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ... | 1993 | 8517721 |
| [optimal survival of bilophila wadsworthia under various transport conditions]. | | 1996 | 8822060 |
| in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria. | the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ... | 1996 | 8878617 |
| taurine reduction in anaerobic respiration of bilophila wadsworthia rzatau. | organosulfonates are important natural and man-made compounds, but until recently (t. j. lie, t. pitta, e. r. leadbetter, w. godchaux iii, and j. r. leadbetter. arch. microbiol. 166:204-210, 1996), they were not believed to be dissimilated under anoxic conditions. we also chose to test whether alkane- and arenesulfonates could serve as electron sinks in respiratory metabolism. we generated 60 anoxic enrichment cultures in mineral salts medium which included several potential electron donors and ... | 1997 | 9143131 |
| [rapid identification of bilophila wadsworthia]. | biophila wadsworthia is a recently recognized nonspore-forming anaerobic gram-negative rod and is reported to be associated with various infections such as gangrenous perforated appendicitis, peritonitis, osteomyelitis and bacteremia. although the isolation of b. wadsworthia seems to be facilitated by using bacteroides bile esculin (bbe) agar, the reliable scheme of identification of this species has been published. this study was conducted to find simple, rapid, and reliable measures for identi ... | 1997 | 9283136 |
| recently described clinically important anaerobic bacteria: medical aspects. | there is still inadequate information on the role of certain newly described or reclassified anaerobes in disease processes, on their normal sites of carriage, and on their antimicrobial susceptibilities. herein, we summarize this information (most of the literature reviewed is from the past 5 years, but a few of the articles are approximately 10 years old). porphyromonas species had seemed to be relatively nonpathogenic, but recent work indicates that this belief is incorrect. p. gingivalis, p ... | 1997 | 9310641 |
| adherence of bilophila wadsworthia to laminin and fibronectin. | | 1997 | 9310670 |
| bilophila wadsworthia clinical isolates compared by polymerase chain reaction fingerprinting. | bilophila wadsworthia isolates recovered from a right-ear cholesteatoma and brain abscess of the same patient were analyzed by means of polymerase chain reaction (pcr) fingerprinting with single primers (t3b and m13 core) to ascertain if they originated from the same clone. their pcr fingerprint profiles were compared with those of three additional b. wadsworthia clinical isolates and the type strain (atcc 49260). the two isolates from the same patient produced pcr fingerprint profiles identical ... | 1997 | 9310711 |
| [first isolation of bilophila wadsworthia in otitis externa]. | bilophila wadsworthia is an obligately anaerobic, gram-negative rod, which was first described in 1989. the natural habitat of this species seems to be the lower intestinal tract where it was recovered from about 60% of the persons investigated. bilophila wadsworthia is mainly associated with intra-abdominal infections, but the species was also isolated as part of aerobic-anaerobic mixed infections at various extra-abdominal sites. otological infections due to bilophila wadsworthia have as yet n ... | 1997 | 9340341 |
| antimicrobial susceptibility testing of bilophila wadsworthia isolates submitted for routine laboratory examination. | mics of antibiotics against bilophila wadsworthia isolates were measured by agar and broth microdilution with pyruvic acid and by etest. the inoculum size influenced greatly agar dilution. despite discrepancies in mics depending on the measurement method used, clindamycin consistently showed potent activity. broth microdilution and etest appear to be candidates for laboratory susceptibility testing. | 1998 | 9620424 |
| susceptibility of the anaerobic gram-negative non-sporulating rod, bilophila wadsworthia to beta-lactams, beta-lactamase inhibitors, meropenem, metronidazole, clindamycin and quinolones. | the susceptibility of eighty-seven strain of bilophila wadsworthia to five beta-lactams, two beta-lactamase inhibitors, meropenem, metronidazole, clindamycin and two quinolones was determined. tests were performed by the modified reference agar dilution technique using triphenyltetrazolium chloride for endpoint reading. the test strains showed a reduced susceptibility to the beta-lactams, penicillin g (mic90 4 micrograms/ml), ampicillin (mic90 32 micrograms/ml), piperacillin (mic90 64 micrograms ... | 1998 | 9638871 |
| bilophila wadsworthia isolated from clinical specimens in taiwan. | bilophila wadsworthia, a newly discovered anaerobic gram-negative species, has been found in such various clinical specimens as bile, pus, blood et cetera, in addition to appendicitis specimens. the formation of black center colonies in bacteroides bile esculin (bbe) agar is one of the important characteristics of the species. | 1994 | 9747333 |
| activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes. | the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ... | 1999 | 10543754 |
| comparison of effects of medium composition and atmospheric conditions on detection of bilophila wadsworthia beta-lactamase by cefinase and cefinase plus methods. | the influence of growth medium and incubation conditions on the detection of bilophila wadsworthia beta-lactamase was tested with cefinase and cefinase plus disks. the tests involved aerobic and anaerobic incubation with conventional disk and quantitative tube assays. the production of beta-lactamase was correlated with penicillin g, ampicillin, and ampicillin-sulbactam mics and inhibition zones on penicillin (2-u) disks. the strains were grown on (i) brucella agar (brucella), (ii) brucella agar ... | 2000 | 10655376 |
| anaerobic bacteraemia: a review of 17 cases. | of 93 blood cultures received with a suspicion of anaerobic bacteraemia over a period of two years, only 17 (18.3%) showed anaerobic growth. twelve grew anaerobes alone while five had a polymicrobial flora. seven of these patients (4.3%) had pre-existing heart disease while others had history of prior surgery, diabetes mellitus or urinary tract infection. oropharynx was the commonest portal of entry, followed by gastrointestinal tract. the anaerobes isolated were anaerobic streptococci, bacteroi ... | 1998 | 10703573 |
| comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria. | the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ... | 2000 | 10795599 |
| in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria. | we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ... | 2000 | 10882699 |
| comparative in vitro activities of ertapenem (mk-0826) against 1,001 anaerobes isolated from human intra-abdominal infections. | by using an agar dilution method, the comparative in vitro activities of ertapenem (mk-0826) were studied against 1,001 anaerobes isolated from human intra-abdominal infections in 17 countries worldwide. mk-0826 was uniformly active against all isolates, including all bacteroides fragilis group species isolates, with the exception of 12 of 61 (20%) strains of bilophila wadsworthia, 3 strains of lactobacilli, and 1 isolate of acidaminococcus fermentans. geographical variation in activity was not ... | 2000 | 10952584 |
| purification, properties and primary structure of alanine dehydrogenase involved in taurine metabolism in the anaerobe bilophila wadsworthia. | alanine dehydrogenase [l-alanine:nad+ oxidoreductase (deaminating), ec 1.4.1.4.] catalyses the reversible oxidative deamination of l-alanine to pyruvate and, in the anaerobic bacterium bilophila wadsworthia rzatau, it is involved in the degradation of taurine (2-aminoethanesulfonate). the enzyme regenerates the amino-group acceptor pyruvate, which is consumed during the transamination of taurine and liberates ammonia, which is one of the degradation end products. alanine dehydrogenase seems to b ... | 2000 | 11041346 |
| biochemical and molecular characterization of taurine:pyruvate aminotransferase from the anaerobe bilophila wadsworthia. | bilophila wadsworthia rzatau is a gram-negative bacterium which converts the sulfonate taurine (2-aminoethanesulfonate) to ammonia, acetate and sulfide in an anaerobic respiration. taurine:pyruvate aminotransferase (tpa) catalyses the initial metabolic reaction yielding alanine and sulfoacetaldehyde. we purified tpa 72-fold to apparent homogeneity with an overall yield of 89%. the purified enzyme did not require addition of pyridoxal 5'-phosphate, but highly active enzyme was only obtained by ad ... | 2000 | 11082195 |
| bacteriology of histopathologically defined appendicitis in children. | acute appendicitis is the most common surgical emergency in childhood. however, the pathogenesis and detailed microbiology are obscure. | 2000 | 11099090 |
| comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria. | the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ... | 2001 | 11120995 |
| a member of the delta subgroup of proteobacteria from a pyogenic liver abscess is a typical sulfate reducer of the genus desulfovibrio. | strain fh26001/95 (atcc 700045) was previously isolated from a pyogenic liver abscess from a human. comparative 16s rrna gene sequence analysis showed that this strain is related to members of the delta subgroup of the proteobacteria, within a cluster of sulfate-reducing bacteria (desulfovibrio spp.) and non-sulfate-reducing bacteria (bilophila wadsworthia and lawsonia spp.). the phenotype of strain fh26001/95 was found to be typical of members of the genus desulfovibrio. growth and substrate tr ... | 2001 | 11158153 |
| dissimilatory sulfite reductase (desulfoviridin) of the taurine-degrading, non-sulfate-reducing bacterium bilophila wadsworthia rzatau contains a fused dsrb-dsrd subunit. | a dissimilatory sulfite reductase (dsr) was purified from the anaerobic, taurine-degrading bacterium bilophila wadsworthia rzatau to apparent homogeneity. the enzyme is involved in energy conservation by reducing sulfite, which is formed during the degradation of taurine as an electron acceptor, to sulfide. according to its uv-visible absorption spectrum with maxima at 392, 410, 583, and 630 nm, the enzyme belongs to the desulfoviridin type of dsrs. the sulfite reductase was isolated as an alpha ... | 2001 | 11160104 |
| in vitro activity of telithromycin (hmr 3647) against 502 strains of anaerobic bacteria. | in a previous study, we compared hmr 3004 with azithromycin, clarithromycin, erythromycin and roxithromycin against 502 anaerobic bacteria using nccls-approved procedures. this report extends this study by reporting the activity of telithromycin (hmr 3647) against these strains. telithromycin inhibited 10% of bacteroides fragilis, 50% of other b. fragilis group organisms and 93% of other bacteroides spp. telithromycin inhibited all porphyromonas spp. and 98% of prevotella spp. activity against b ... | 2001 | 11266423 |
| colonic infection by bilophila wadsworthia in pigs. | bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. challenge-exposure or prevalence studies in laboratory and other animals have not been reported. b. wadsworthia is closely related phylogenetically to desulfovibrio sp. and lawsonia intracellularis, which are considered colon pathogens. we developed a pcr specific for b. wadsworthia dna. samples of bacterial dna extracted from the feces of pig ... | 2001 | 11283090 |
| genetic analysis of a rhodobacter capsulatus gene region involved in utilization of taurine as a sulfur source. | rhodobacter capsulatus was shown to grow efficiently with taurine as sole source of sulfur. we identified a gene region exhibiting similarity to the escherichia coli tauabc genes coding for a taurine-specific abc transporter. the r. capsulatus tauabc genes were flanked by two putative operons (orf459-484-590 and cyse-srpi-nifs2) both reading in opposite direction relative to tauabc. orf459 shows strong similarity to taurine:pyruvate aminotransferase (tpa) from bilophila wadsworthia catalyzing th ... | 2001 | 11728723 |
| antimicrobial susceptibility of non-bacteroides fragilis group anaerobic gram-negative bacilli in europe. | objective: to investigate the in vitro activity of a range of anti-anaerobe antimicrobials against non-bacteroides fragilis group anaerobic gram-negative bacilli isolated in europe. methods: isolates from 15 laboratories in 13 countries were identified by conventional methods. the mics of 20 antibiotics were determined by an agar dilution method. results: there were 488 prevotella spp., 174 fusobacteria, 69 porphyromonas spp., 33 bacteroides spp., 28 bilophila wadsworthia and 16 campylobacter sp ... | 1999 | 11853565 |
| in vitro activity of gemifloxacin compared to seven other oral antimicrobial agents against aerobic and anaerobic pathogens isolated from antral sinus puncture specimens from patients with sinusitis. | using a microbroth method for aerobes and agar dilution for anaerobes, we studied the comparative in vitro activity of gemifloxacin, three fluoroquinolones, two macrolides and two beta-lactams, against 207 aerobic and 162 anaerobic antral sinus puncture isolates. gemifloxacin was active at < or = 0.25 ug/ml against 198/207 [96%] aerobes and 127/162 [78%] anaerobes or 325/369 [88%] of all isolates and was the most active quinolone on a weight basis against gram-positive organisms. all haemophilus ... | 2002 | 11858906 |
| incidence of bilophila wadsworthia in appendiceal, peritoneal and fecal samples from children. | | 1997 | 11864089 |
| comparison of the etest and a microbroth dilution system (sceptor) to a reference agar dilution method for susceptibility testing of bilophila wadsworthia. | objective: to compare the etest and a microbroth dilution system (sceptor) to a reference agar dilution method for susceptibility testing of bilophila wadsworthia. methods: the susceptibility of 15 clinical isolates of bilophila wadsworthia was determined by the national committee for clinical laboratory standards (nccls) agar dilution method using triphenyltetrazolium chloride for endpoint determination. the results were compared with the results obtained by the e test and a commercial microbro ... | 1997 | 11864112 |
| in vitro activities of faropenem against 579 strains of anaerobic bacteria. | the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ... | 2002 | 12384389 |
| search for lawsonia intracellularis and bilophila wadsworthia in malabsorption-diseased chickens. | proliferative enteropathy is an important enteric disease caused by lawsonia intracellularis. a wide range of host species can be infected by the same bacterium, yet the clinico-pathologic features among these hosts remains almost identical. the disease has been recognized regularly among ratites, but not in other avian families, such as galliforms, even though these suffer uncharacterized enteric conditions. fresh ileum-colon contents were obtained from 228, 3- to 8-week-old chickens with enter ... | 2003 | 12889732 |
| preparation and characterization of polyclonal and monoclonal antibodies against lawsonia intracellularis. | proliferative enteropathy is an intestinal infectious disease caused by the obligate intracellular bacterium lawsonia intracellularis. immunohistochemistry staining has superior sensitivity over hematoxylin and eosin and silver staining for detecting l. intracellularis in histological sections. a l. intracellularis-specific monoclonal antibody (mab) produced in the uk (ig4 mab) has been described in the literature. however, no monoclonal or polyclonal antibodies are commercially available. there ... | 2003 | 14535543 |
| in vitro activities of a new des-fluoro(6) quinolone, garenoxacin, against clinical anaerobic bacteria. | the antimicrobial activities of garenoxacin and eight other antibiotics against 641 anaerobic isolates were evaluated with the nccls agar dilution method. overall, the mics of garenoxacin for 50 and 90% of the strains tested (in micrograms per milliliter) were as follows: bacteroides fragilis group, 0.5 and 2; prevotella spp., 0.25 and 2; fusobacterium spp., 0.25 and 0.5; porphyromonas spp., 0.125 and 0.25; bilophila wadsworthia, 0.5 and 1; veillonella spp., 0.25 and 0.5; clostridium spp., 0.25 ... | 2003 | 14576144 |
| two intriguing bilophila wadsworthia cases from hungary. | bilophila wadsworthia, an obligately anaerobic gram-negative bacillus, was first isolated from appendicitis specimens and human faeces. the two cases described here are the first report of the isolation of b. wadsworthia in hungary, and include the first isolation from chronic mastoiditis complicated with a brain abscess. | 2004 | 15496398 |
| biochemical differentiation and comparison of desulfovibrio species and other phenotypically similar genera. | seventeen human clinical isolates representing four species of desulfovibrio were characterized using 16s rrna gene sequences and tests for catalase, indole, nitrate, bile, urease, formate-fumarate stimulation, desulfoviridin, motility, and hydrogen sulfide production, plus susceptibility to antimicrobial agents. eighty additional strains representing 10 phenotypically similar genera (bilophila, selenomonas, capnocytophaga, campylobacter, bacteroides, sutterella, anaerobiospirillum, dialister, v ... | 2005 | 16081948 |
| in vitro activity of ramoplanin and comparator drugs against anaerobic intestinal bacteria from the perspective of potential utility in pathology involving bowel flora. | susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infections and for certain other situations in which intestinal bacteria may play a role. the antimicrobial activity of ramoplanin (mdl 62,198) against 928 strains of intestinal anaerobic ... | 2004 | 16701520 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004]. | tendency of isolated bacteria from infections in general surgery during the period from april 2004 to march 2005 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 645 strains including 17 strains of candida spp. were isolated from 226 (79.0%) of 286 patients with surgical infections. three hundred and seventeen strains were isolated from primary infections, and 345 strains were isolated from postoperative infections. from primary infectio ... | 2006 | 16805318 |
| identification of bilophila wadsworthia by specific pcr which targets the taurine:pyruvate aminotransferase gene. | the bile-resistant, strictly anaerobic bacterium bilophila wadsworthia is found in human faecal flora, in human infections and in environmental samples. a specific pcr primer set for the gene encoding the first metabolic enzyme in the degradative pathway for taurine in b. wadsworthia, taurine:pyruvate aminotransferase (tpa), was developed and tested. in addition, enrichment cultures were started from faecal samples of primates and felines and shown to contain b. wadsworthia. these were subcultur ... | 2006 | 16842362 |
| preliminary evidence of endotoxic activity of bilophila wadsworthia. | in this study we describe two properties of the gram-negative bacterium bilophila wadsworthia, namely the ability to clot limulus lysate and the capacity to induce the production of tissue factor-like procoagulant activity by human mononuclear cells in vitro. although exhibited at a lower degree when compared with those of typical gram-negative bacteria or gram-negative endotoxin those activities may account in part for bilophila's pathogenicity. the capacity indeed to induce fibrin formation th ... | 1995 | 16887503 |
| bilophila wadsworthia: a unique gram-negative anaerobic rod. | although comprising less than 0.01% of the normal human gastrointestinal microbiota, bilophila wadsworthia is the third most common anaerobe recovered from clinical material obtained from patients with perforated and gangrenous appendicitis. since its discovery in 1988, b. wadsworthia has been recovered from clinical specimens associated with a variety of infections, including sepsis, liver abscesses, cholecystitis, fournier's gangrene, soft tissue abscesses, empyema, osteomyelitis, bartholiniti ... | 1997 | 16887567 |
| bilophila wadsworthiain brain abscess: case report. | a case of a patient with a 20-year history of chronic otitis media complicated by cholesteatoma and brain abscess is described. a ct scan with contrast material showed three abscess cavities in the right cerebellar hemisphere. a culture from a specimen of the cholesteatoma yielded a significant amount of growth of bilophila wadsworthia, bacteroides fragilis and prevotella oris and a moderate growth of alpha-streptococci and staphylococcus simulans. from the pus of the brain abscess we also isola ... | 1997 | 16887572 |
| recovery of bilophila wadsworthiafrom clinical specimens in italy. | this report is the first survey in italy to evaluate the incidence of recovery of bilophila wadsworthia in clinical situations. the survey was carried out at the departments of microbiology in two northern italian hospitals over a one-year period. tests for b. wadsworthia were carried out on a range of specimens from different body sites, when etiology by anaerobes was suspected. out of a total of 350 samples examined, 67% were positive in bacteriological tests. mixed anaerobic infections were d ... | 1997 | 16887594 |
| adherence of bilophila wadsworthia to cultured human embryonic intestinal cells. | adherence of bilophila wadsworthia to the cultured human embryonic intestinal cell line, intestine 407 (int 407), varied among the strains tested from strongly adherent (76-100% cells positive for one or more adherent bacteria) to non- or weakly adherent (0-25% positive cells). although negative staining revealed that infrequent cells of an adherent strain, wal 9077, the adherent type-strain, wal 7959, and a non-adherent strain, wal 8448, expressed loosely associated fimbrial structures, a role ... | 1998 | 16887620 |
| effects of host iron transport compounds on growth kinetics and outer-membrane protein expression of bilophila wadsworthia. | since the environmental iron concentration has emerged as an important attribute in the expression of bacterial virulence, the purpose of this study was to determine the effects of transferrin, lactoferrin, heme compounds, and inorganic iron sources (ferric and ferrous sulfate) on the growth of bilophila wadsworthia and to study its outer membrane composition when grown under these different simulated in vivo conditions. lactoferrin, transferrin, hemin and hemoglobin supported full growth of the ... | 1998 | 16887629 |
| deoxycholic acid formation in gnotobiotic mice associated with human intestinal bacteria. | in humans and animals, intestinal flora is indispensable for bile acid transformation. the goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice inoculated with a human fecal dilution of 10(-6), and five strains of 7alpha-dehydroxylating bac ... | 2006 | 17152920 |
| [bilophila wadsworthia isolated from surgical specimens]. | bilophila wadsworthia is a recently recognized nonspore-forming anaerobic gram-negative rod and is reported to be associated with various surgical infections. in the present study, the isolation rate of b. wadsworthia from surgical specimens and its antimicrobial susceptibilities were examined. last 5 years, b. wadsworthia was isolated from 46 (5.2%) out of 884 specimens from the surgical abdominal infections. b. wadsworthia was most frequently isolated from secondary peritonitis such as perfora ... | 2006 | 17334062 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004]. | tendency of isolated bacteria from infections in abdominal surgery during the period from april 2005 to march 2006 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 384 strains including 18 strains of candida spp. were isolated from 161 (70.3%) of 229 patients with surgical infections. one hundred and ninty-five strains were isolated from primary infections, and 171 strains were isolated from postoperative infections. from primary infecti ... | 2007 | 17612256 |
| [application of "microring an" to "level 1": presumptive identification of anaerobic gram-negative bacilli]. | commonly isolated anaerobic gram-negative rods (4 genus 64 strains), some other important gram-negative anaerobic species (9 genus 45 strains), and cigar-shaped clostridia (11 strains) were studied on their susceptibility patterns to 6 agents on "microring an". some modifications were made in the methods and interpretation of results. susceptibility patterns to erythromycin, rifampicin, colistin, benzylpenicillin, kanamycin, and vancomycin were following (sensitive [s], intermediate [i], resista ... | 2006 | 17822332 |
| [bacteria isolated from perforation peritonitis and their antimicrobial susceptibilities]. | bacteria isolated from 25 cases of perforation peritonitis and its antimicrobial susceptibilities were studied. one hundred and forty-six strains including 36 strains of aerobic bacteria, 107 strains of anaerobic bacteria and 3 strains of yeast were isolated from intra-peritoneal fluid obtained at operation. among aerobic bacteria, enterobacteriaceae was isolated from 13 cases (52%) out of all, and followed by those of enterococcus spp. (24%), pseudomonas aeruginosa (20%), in this order. among n ... | 2007 | 18018418 |
| hydrogen as an energy source for the human pathogen bilophila wadsworthia. | the gram-negative anaerobic gut bacterium bilophila wadsworthia is the third most common isolate in perforated and gangrenous appendicitis, being also found in a variety of other infections. this organism performs a unique kind of anaerobic respiration in which taurine, a major organic solute in mammals, is used as a source of sulphite that serves as terminal acceptor for the electron transport chain. we show here that molecular hydrogen, one of the major products of fermentative bacteria in the ... | 2008 | 18066702 |
| isolation and characterization of porins from desulfovibrio piger and bilophila wadsworthia: structure and gene sequencing. | the outer membrane proteins of desulfovibrio piger and bilophila wadsworthia (omp-dp and omp-bw, respectively) and the genes encoding them (omp-dp and omp-bw) were isolated and characterized. native omp-dp and omp-bw form a trimeric structure of approximately 120 kda. these proteins disaggregated into monomers with a molecular weight of approximately 53 kda after heating at 95 degrees c for 10 min. the pore-forming abilities of these oligomeric proteins demonstrated that they form small nonspeci ... | 2008 | 18709355 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2006 and march 2007]. | tendency of isolated bacteria from infections in abdominal surgery during the period from april 2006 to march 2007 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 474 strains including 23 strains of candida spp. were isolated from 170 (75.2%) of 226 patients with surgical infections. two hundred and twenty-six strains were isolated from primary infections, and 224 strains were isolated from postoperative infections. from primary infecti ... | 2008 | 18814799 |
| [bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008]. | bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ... | 2009 | 19860320 |
| [bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents --special references to bacteria isolated between april 2008 and march 2009]. | bacteria isolated from infections in abdominal surgery during the period from april 2008 to march 2009 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 712 strains including 18 strains of candida spp. were isolated from 173 (80.5%) of 215 patients with surgical infections. three hundred and sixty-six strains were isolated from primary infections, and 346 strains were isolated from postoperative infections. from primary infections, anaero ... | 2010 | 20919496 |
| abundance and diversity of mucosa-associated hydrogenotrophic microbes in the healthy human colon. | hydrogenotrophic microbiota have a significant impact on colonic health; however, little is known about their diversity and ecology in situ. here, molecular-based methods and multivariate analyses were used to examine the abundance and diversity of mucosa-associated hydrogenotrophic microbes in 90 biopsies collected from right colon, left colon and rectum of 25 healthy subjects. functional genes of all three hydrogenotrophic groups were detected in at least one colonic region of all subjects. me ... | 2011 | 21753800 |
| [Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between April 2009 and March 2010]. | Bacteria isolated from surgical infections during the period from April 2009 to March 2010 were investigated in a multicenter study in Japan, and the following results were obtained. In this series, 671 strains including 16 strains of Candida spp. were isolated from 174 (79.1%) of 220 patients with surgical infections. Four hundred and eleven strains were isolated from primary infections, and 244 strains were isolated from surgical site infection. From primary infections, anaerobic Gram-negative ... | 2011 | 21861307 |
| Desulfobaculum xiamenensis gen. nov., sp. nov., a member of the family Desulfovibrionaceae isolated from marine mangrove sediment. | A taxonomic study was carried out on strain P1(T), which was isolated from mangrove sediment samples collected from Qinglan Port (Hainan, China). Cells were curved rods, motile with a single polar flagellum. The strain was non-spore-forming with cell size of 0.6 × 1.5-2.2 µm. Catalase and oxidase activities were not detected. Growth was observed in the temperature range 22-44 °C (optimum 35-40 °C) and pH range 5.5-8.5 (optimum 7.0). NaCl was required for growth, and tolerated up to 3.5% (op ... | 2011 | 21873514 |