| helicobacter pullorum sp. nov.-genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. | campylobacter-like organisms were isolated from the liver, duodenum and caecum of broiler and layer chickens, and from humans with gastroenteritis. they formed a unique dna homology group and a polyphasic taxonomic analysis was made of 16 strains. analysis of the nucleotide sequence of the 16s rrna gene from seven of the strains identified them as belonging to a single species, within the genus helicobacter. this conclusion was supported by the studies of relative dna homology and of total prote ... | 1994 | 7533595 |
| gastroenteritis associated with helicobacter pullorum. | | 1994 | 7983966 |
| helicobacter rodentium sp. nov., a urease-negative helicobacter species isolated from laboratory mice. | a spiral-shaped bacterium with bipolar, single, nonsheathed flagella was isolated from the intestines of laboratory mice. the organism grew at 37 and 42 degrees c under microaerobic and anaerobic conditions, did not hydrolyze urea, was weakly positive for catalase and oxidase, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and was resistant to cephalothin and nalidixic acid. this is the first urease-negative, murine helicobacter spp. isolated from intestines. also, h ... | 1997 | 9226892 |
| isolation of helicobacter pullorum from patients with enteritis. | helicobacter pullorum, recently described as sp. nov., is commonly isolated from asymptomatic poultry. two cases of human enteritis associated with h. pullorum, one of them in an immunocompromised patient, are reported. problems in the correct species identification by means of phenotypic and genotypic methods are discussed and for the first time a fatty acid pattern of helicobacter pullorum is presented. | 1997 | 9255900 |
| comparison of the productivity of cefoperazone amphotericin teicoplanin (cat) agar and modified charcoal cefoperazone deoxycholate (mccd) agar for various strains of campylobacter, arcobacter and helicobacter pullorum. | cefoperazone amphotericin teicoplanin (cat) agar was developed from cefoperazone deoxycholate (mccd) agar by modification of the selective antibiotics in order to permit growth of strains of campylobacter upsaliensis. in this study, 35 strains of campylobacter and arcobacter were tested for their ability to grow on cat and mccd media using the ecometric method. six of these strains were also tested using the modified miles-misra method. overall, nineteen strains out of the 35 tested grew better ... | 1997 | 9506285 |
| identification of unusual campylobacter-like isolates from poultry products as helicobacter pullorum. | twenty-six unclassified campylobacter-like strains previously isolated from 15 chicken carcasses and caecal contents, together with two more strains isolated from chicken faeces on a different occasion, were identified as helicobacter pullorum using various phenotypic identification methods. api campy identification kits and a 16-test identification scheme developed for campylobacters failed to identify these bacteria, or identified them as campylobacter spp. eighteen strains (including the two ... | 1998 | 9717286 |
| discrimination of helicobacter pullorum and campylobacter lari by analysis of whole cell fatty acid extracts. | helicobacter pullorum and campylobacter lari are rarely isolated from humans with acute enteritis. hitherto the two species could only be identified by genotypic techniques. gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. cholesteryl glucoside, a characteristic feature of the genus helicobacter, but seldom found in other bacteria, could not be detected in helicobacter pullorum. therefore, rapid determi ... | 1998 | 9835030 |
| routine identification of campylobacter jejuni and campylobacter coli from human stool samples. | correct identification of campylobacter jejuni and campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. the widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are not generally available. this investigation was performed to assess the performance of common biochemical identification in comparison with species-specific pcr and gas liquid chro ... | 1999 | 10518720 |
| genetic diversity in helicobacter pullorum from human and poultry sources identified by an amplified fragment length polymorphism technique and pulsed-field gel electrophoresis. | helicobacter pullorum was first isolated from the faeces and carcasses of poultry and has been associated with human gastroenteritis. the aim of this study was to examine interstrain genetic diversity within h. pullorum. two fingerprinting techniques were used: amplified fragment length polymorphism (aflp) and pulsed field gel electrophoretic (pfge) analysis. the 20 strains examined were from four countries and comprised 13 human isolates and seven poultry isolates. their identity was confirmed ... | 1999 | 10583689 |
| identification of helicobacter pylori and other helicobacter species by pcr, hybridization, and partial dna sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. | helicobacter pylori was identified in human liver tissue by pcr, hybridization, and partial dna sequencing. liver biopsies were obtained from patients with primary sclerosing cholangitis (n = 12), primary biliary cirrhosis (n = 12), and noncholestatic liver cirrhosis (n = 13) and (as controls) normal livers (n = 10). pcr analyses were carried out using primers for the helicobacter genus, helicobacter pylori (the gene encoding a species-specific 26-kda protein and the 16s rrna), helicobacter bili ... | 2000 | 10698999 |
| helicobacter canadensis sp. nov. isolated from humans with diarrhea as an example of an emerging pathogen. | we recently analyzed 11 helicobacter isolates cultured from diarrhea patients in canada. these isolates had been characterized biochemically by restriction fragment length polymorphism (rflp; alui, hhai) analysis and by fatty-acid analysis as helicobacter pullorum. however, four of the isolates differed biochemically from h. pullorum by their inability to hydrolyze indoxyl acetate and their resistance to nalidixic acid. using complete 16s rrna analysis, we determined that these four strains clus ... | 2000 | 10878041 |
| slaughter pigs are commonly infected by closely related but distinct gastric ulcerative lesion-inducing gastrospirilla. | an association between (unculturable) gastrospirillum-like organisms (glo) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. in dogs glo detected by microscopy may represent several helicobacter species or subspecies. therefore we investigated which helicobacter spp. are present in stomachs of swine and their possible association with ulcerative lesions of the pars oesophagea. the presence of helicobacter spp. in the antrum and pars oesophagea in 122 stomachs o ... | 2000 | 10878060 |
| cytolethal distending toxin in avian and human isolates of helicobacter pullorum. | helicobacter pullorum has been isolated from the feces and livers of poultry and is associated with human gastroenteritis. discrimination of this organism from other enterohepatic helicobacter species and campylobacter species has proven difficult. h. pullorum from both avian and human clinical sources has dna sequence homology and cytotoxic activity that represent a new member of the cytolethal distending toxin (cdt) family of bacterial toxins. cdt is a potential virulence factor in h. pullorum ... | 2000 | 10915100 |
| differentiation of clinical helicobacter pullorum isolates from related helicobacter and campylobacter species. | helicobacter pullorum, first detected in the liver and intestinal contents of poultry, was defined as a new species in 1994. this organism has since been isolated from humans with gastroenteritis. phenotypic as well as genotypic methods have been used to identify h. pullorum associated with cases of human disease. | 2000 | 10971678 |
| helicobacters of possible zoonotic origin: a review. | since the isolation of helicobacter pylori, many new helicobacter species have been identified from the gastrointestinal tract in humans and animals. in humans, a spiral organism different from h. pylori and provisionally named "helicobacter heilmannii", has been associated with gastritis, gastric ulceration and to a lesser degree, gastric cancer. in addition helicobacter cinaedi, helicobacter fennelliae, helicobacter pullorum and "flexispira rappini" have been isolated from cases of enteric dis ... | 2000 | 11233522 |
| bacteremia caused by a helicobacter pullorum-like organism. | we report a case of bacteremia caused by a helicobacter pullorum-like organism in a 35-year-old man with pyrexia of unknown origin. culture of blood samples obtained at admission yielded a motile, spiral-shaped gram-negative rod, and 16s rrna gene sequencing identified this organism as helicobacter pullorum-like, showing 23 base differences compared with the recently described "helicobacter canadensis" (a recently proposed group that had previously been classified within h. pullorum). we believe ... | 2001 | 11595983 |
| influence of activated charcoal, porcine gastric mucin and beta-cyclodextrin on the morphology and growth of intestinal and gastric helicobacter spp. | bile-tolerant helicobacter spp. are emerging human and animal pathogens. however, due to their fastidious nature, which requires nutrient-rich complex media to grow, infection with these bacteria may be underestimated. the accumulation of toxic metabolites in cultures may be one of the main obstacles for successful culture of these organisms. the present study examined various potential growth-enhancing substances for helicobacter spp. and, furthermore, how they may affect spiral to coccoid conv ... | 2002 | 11882701 |
| immunogenic proteins of helicobacter pullorum, helicobacter bilis and helicobacter hepaticus identified by two-dimensional gel electrophoresis and immunoblotting. | the ecological niches occupied by various species of helicobacter are not yet known and the full spectrum of diseases associated with helicobacter infections are not yet defined. since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. immunogenic cell surface proteins of helicobacter pullorum, helicobacter bili ... | 2002 | 12112861 |
| immune responses to bile-tolerant helicobacter species in patients with chronic liver diseases, a randomized population group, and healthy blood donors. | bile-tolerant helicobacter species such as helicobacter pullorum, helicobacter bilis, and helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (cld) in humans. antibody responses to cell surface proteins of h. pullorum, h. bilis, and h. hepaticus in serum samples from patients with cld, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (elisa). the ... | 2002 | 12414744 |
| the rapid detection of low molecular mass proteins differentially expressed under biological stress for four helicobacter spp. using proteinchip technology. | helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. in addition h. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from h. ... | 2003 | 12627380 |
| potential for proteomic profiling of helicobacter pylori and other helicobacter spp. using a proteinchip array. | the helicobacter genus is associated with a wide spectrum of pathologies in the gastrointestinal tract. however, in contrast to helicobacter pylori, few data are available regarding proteomic characteristics of enterohepatic helicobacters. proteomic analysis of this genus has predominantly utilised two-dimensional gel electrophoresis methodology. in the present study we applied an innovative technique using proteinchip arrays coupled with surface-enhanced laser desorption/ionisation time of flig ... | 2003 | 12738385 |
| genotypic identification of erythromycin-resistant campylobacter isolates as helicobacter species and analysis of resistance mechanism. | the correct identification of campylobacter species remains cumbersome, especially when conventional biochemical tests and antimicrobial susceptibility patterns are used for a phenotypical identification. correct identification is important for epidemiological purposes and for studying changes in antimicrobial resistance patterns. six erythromycin-resistant campylobacter strains were investigated by 16s ribosomal dna (rdna) sequencing, 23s rdna sequencing, and restriction fragment length polymor ... | 2003 | 12904383 |
| identification of campylobacteria isolated from danish broilers by phenotypic tests and species-specific pcr assays. | to validate a phenotypic campylobacter species identification method employed to identify campylobacters in broilers by comparison with campylobacterial species identification using various species-specific pcr analyses. | 2003 | 12969276 |
| increased prevalence of seropositivity for non-gastric helicobacter species in patients with autoimmune liver disease. | various helicobacter species have been isolated from the stomach, intestinal tract and liver of a variety of mammalian and some avian species, and helicobacter dna has been detected in human bile and liver samples. an immunoblot assay was established to analyse serum antibody responses to non-gastric helicobacter species in patients with autoimmune liver diseases, in comparison with healthy individuals. sera from 36 patients with primary sclerosing cholangitis (psc), 21 with primary biliary cirr ... | 2003 | 14532338 |
| helicobacter species sequences in liver samples from patients with and without hepatocellular carcinoma. | only a minority of patients carrying a defined viral aetiologic agent develop cirrhosis and ultimately hepatocellular carcinoma (hcc), the mechanism underlying the worsening is still undefined. experimental infection by helicobacter hepaticus in mice causes chronic hepatitis and hcc and recently, more helicobacter species (helicobacter spp.) have been detected in the liver of patients suffering from cholestatic diseases and hcc arising from non-cirrhotic liver. we investigated whether helicobact ... | 2004 | 14966925 |
| comparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters. | the lipopolysaccharide of helicobacter pylori plays an important role in colonization and pathogenicity. the present study sought to compare structural and biological features of lipopolysaccharides from gastric and enterohepatic helicobacter spp. not previously characterized. | 2004 | 15270745 |
| association of helicobacter species with hepatitis c cirrhosis with or without hepatocellular carcinoma. | recent studies have suggested that bacterial coinfection with helicobacter species in patients already infected with hepatitis c virus (hcv) could be involved in the development of cirrhosis and hepatocellular carcinoma (hcc). a retrospective cross sectional study was performed in order to explore the association between helicobacter species and hcv associated liver diseases. | 2005 | 15710989 |
| prevalence of helicobacter pullorum among patients with gastrointestinal disease and clinically healthy persons. | feces from 531 patients with gastroenteritis and from 100 clinically healthy individuals were tested for helicobacter pullorum by use of pcr. samples positive by pcr were qualified for isolation. h. pullorum dna was demonstrated to be present in feces from 4.3% of patients with gastrointestinal disease but also in feces from 4.0% of clinically healthy persons. one strain was isolated from one patient with gastrointestinal disease. | 2005 | 15956438 |
| the cytolethal distending toxin among helicobacter pullorum strains from human and poultry origin. | helicobacter pullorum has been associated with diarrhoea, gastroenteritis and liver disease in humans and with hepatitis and enteritis in poultry. the purpose of the present study was to examine whether cytolethal distending toxin was present among 10 poultry and three human h. pullorum isolates and whether a different level of cytolethal distending toxin activity was noted. a pcr assay was performed to detect the cdtb gene. in addition, epithelial hep-2 cells inoculated with sonicate from all s ... | 2006 | 16310982 |
| helicobacter pullorum in chickens, belgium. | a total of 110 broilers from 11 flocks were tested for helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. h. pullorum isolates were examined by amplified fragment length polymorphism (aflp) fingerprinting for interstrain genetic diversity and relatedness. sixteen isolates from cecal samples from 2 different flocks were obtained. aflp analysis showed that these isolates and 4 additional isolates from a different flock cluster ... | 2006 | 16494752 |
| detection of helicobacter pullorum in meat chickens in australia. | | 2006 | 16555556 |
| detection and identification of food-borne pathogens of the genera campylobacter, arcobacter and helicobacter by multiplex pcr in poultry and poultry products. | the objective of this study was to develop a multiplex polymerase chain reaction (pcr) to detect and differentiate food-borne pathogens of the three genera campylobacter, arcobacter and helicobacter in a single step procedure. one common reverse primer and three genus-specific forward primers were designed by hybridizing to the 16s rrna of selected reference strains. besides the species with significance as food-borne pathogens isolated from poultry meat--campylobacter jejuni, campylobacter coli ... | 2006 | 17010041 |
| helicobacter equorum sp. nov., a urease-negative helicobacter species isolated from horse faeces. | gram-negative, curved, motile bacteria (strains eqf1t and eqf2) were isolated from faecal samples from two clinically healthy horses. both strains possessed a single, monopolar, sheathed flagellum and were urease-negative. the novel strains grew at 37 degrees c under microaerobic conditions and were positive for oxidase, catalase and alkaline phosphatase activities. the isolates reduced nitrate to nitrite, but gamma-glutamyl transpeptidase activity was not detected. the novel isolates did not gr ... | 2007 | 17267952 |
| tissue tropism of helicobacter pullorum in specific pathogen-free chickens determined by culture and nucleic acid detection. | three-day-old specific pathogen-free chickens (n = 24) located in isolators were inoculated orally with helicobacter pullorum. one group (n = 12) was infected with a h. pullorum field isolate from human origin, another one (n = 12) with the american type culture collection h. pullorum reference isolate 51801 originating from chickens. both isolates were positive for cytolethal distending toxin, investigated using a polymerase chain reaction (pcr). a third group (n = 4) was kept as a negative con ... | 2006 | 17274304 |
| pathogenesis of helicobacter pullorum infections in broilers. | four groups of 23 one-day-old broiler chickens were each inoculated by gavage with a different helicobacter pullorum strain isolated from humans or poultry. as a control, a fifth group of eight animals was inoculated with phosphate-buffered saline. faecal samples were collected weekly and tested for the presence of h. pullorum dna using pcr. at 1, 8, 15, 22 and 42 days postinoculation, birds were euthanized and samples from the liver and intestinal tract were histologically, immunohistochemicall ... | 2007 | 17289200 |
| occurrence and antibiotic susceptibility of helicobacter pullorum from broiler chickens and commercial laying hens in italy. | in 2005, in order to investigate the occurrence of helicobacter pullorum in poultry, the caecal contents collected from a total of 60 animals intensively reared in italy on 15 different farms (9 farms of broiler chicken and 6 of laying hens) were examined at the slaughterhouse. a modified steele-mcdermott membrane filter method was used. small, greyish-white colonies of gram-negative, gently curved, slender rod bacteria were preliminarily identified as h. pullorum by a polymerase chain reaction ... | 2007 | 17303278 |
| first report of helicobacter pullorum in the faeces of a diarrhoeic psittacine bird (psephotus haematogaster). | | 2006 | 16980526 |
| nucleotide sequence of the gyra gene of arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates. | the nucleotide sequence of the gyra gene of arcobacter butzleri, arcobacter cryaerophilus, arcobacter cibarius, and arcobacter skirrowii was determined. the deduced gyra proteins are closely related to those of wolinella succinogenes and helicobacter pullorum, whereas those of campylobacter species showed less sequence identity. the phylogenetic analysis of gyra sequences provides a result similar to 16s rrna gene sequence phylogenetic analysis and allows the discrimination among a. butzleri spe ... | 2007 | 17378897 |
| serum antibodies to enterohepatic helicobacter spp. in patients with chronic liver diseases and in a population with high prevalence of h. pylori infection. | enteric helicobacter species might be a risk factor for chronic liver and biliary tract diseases. | 2006 | 16464652 |
| new trends in emerging pathogens. | the emergence of pathogens is the result of a number of impact in all parts of the food chain. the emerging technologies in food production explain how new pathogens can establish themselves in the food chain and compromise food safety. the impact of the food technology is analysed for several bacteria, such as yersinia, campylobacter, arcobacter, helicobacter pullorum, enterobacter sakazakii, mycobacterium avium spp. paratuberculosis, prions related to vcjd and others. the importance of the abi ... | 2007 | 17976849 |
| development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed populations. | the identification of sites resulting in cross-contamination of poultry flocks in the abattoir and determination of the survival and persistence of campylobacters at these sites are essential for the development of intervention strategies aimed at reducing the microbial burden on poultry at retail. a novel molecule-based method, using strain- and genus-specific oligonucleotide probes, was developed to detect and enumerate specific campylobacter strains in mixed populations. strain-specific oligo ... | 2008 | 18281428 |
| study of helicobacter pullorum proinflammatory properties on human epithelial cells in vitro. | helicobacter pullorum is an enterohepatic helicobacter species of avian origin detected in patients with acute diarrhoea and inflammatory bowel disease. the aim of the present study was to determine whether h pullorum exerts a direct effect on human intestinal epithelial cells in vitro and to characterise the bacterial mechanisms and the signalling pathways involved. | 2009 | 18579667 |
| in vitro susceptibility of helicobacter pullorum strains to different antimicrobial agents. | the in vitro activity of 13 antimicrobial agents against 23 helicobacter pullorum strains from poultry (21) and human (two) origin, and one human h. canadensis strain was tested by the agar dilution method. with the h. pullorum strains, monomodal distributions of minimum inhibitory concentrations (mics) were seen with lincomycin, doxycycline, gentamicin, tobramycin, erythromycin, tylosin, metronidazole, and enrofloxacin in concentration ranges considered as indicating susceptibility in other bac ... | 2005 | 15910225 |
| phylogenetic analysis of helicobacter species based on partial gyrb gene sequences. | analysis of 16s rrna gene sequences is one of the most common methods for investigating the phylogeny and taxonomy of bacteria. however, several studies have indicated that the 16s rrna gene does not distinguish between certain helicobacter species. we therefore selected for phylogenetic analysis an alternative marker, gyrb, encoding gyrase subunit b. the aim of this investigation was to examine the applicability of gyrb gene fragments (approximately 1100 bp) for the phylogenetic study of 16 hel ... | 2007 | 17329766 |
| mitotic catastrophe as a prestage to necrosis in mouse liver cells treated with helicobacter pullorum sonicates. | helicobacter pullorum infections have been associated with several enterohepatic diseases, but the mechanism of action is currently undefined. the present study was therefore set up to investigate possible cytotoxic effects of this pathogen on liver cells. a mouse hepatic cell line was exposed to h. pullorum sonicate and cytotoxicity was observed for all isolates after incubation for 72 h. features characteristic for mitotic catastrophe characterized by chromatin condensation, formation of multi ... | 2009 | 19217023 |
| complete nucleotide sequence of the gyra gene of helicobacter pullorum and identification of a point mutation leading to ciprofloxacin resistance in poultry isolates. | to assess the molecular basis of nalidixic acid and ciprofloxacin resistance in helicobacter pullorum, the gyra gene of h. pullorum cip 104787t was sequenced. in addition, 9 isolates (2 susceptible to ciprofloxacin and resistant to nalidixic acid, 3 susceptible and 4 resistant to both antibiotics) were selected from 44 poultry isolates and the nucleotide sequences of their quinolone resistance-determining regions (qrdrs) were compared. the 2490 bp gyra gene showed an open reading frame encoding ... | 2007 | 17644320 |
| species-specific identification and differentiation of arcobacter, helicobacter and campylobacter by full-spectral matrix-associated laser desorption/ionization time of flight mass spectrometry analysis. | rapid and reliable identification of arcobacter and helicobacter species, and their distinction from phenotypically similar campylobacter species, has become increasingly important, since many of them are now recognized as human and/or animal pathogens. matrix-associated laser desorption/ionization-time of flight (maldi-tof) ms has been shown to be a rapid and sensitive method for characterization of micro-organisms. in this study, we therefore established a reference database of selected arcoba ... | 2010 | 19959629 |
| association between entero-hepatic helicobacter species and crohn's disease: a prospective cross-sectional study. | the pathogenesis of crohn's disease (cd) involved microbial factors. some helicobacter species, the so-called entero-hepatic helicobacters (ehh), can naturally colonize the intestinal surface and have been detected in humans. aim to look for an association between cd and the presence of ehh dna in intestinal biopsies. | 2009 | 19438427 |
| high levels of serum antibodies to cell surface proteins of helicobacter pullorum and helicobacter pylori in hepatitis c virus-infected patients. | | 2009 | 18821131 |
| a novel real-time pcr assay for the detection of helicobacter pullorum-like organisms in chicken products. | a novel real-time pcr assay was developed for the direct detection in food of helicobacter pullorum-like bacteria, which are occasionally associated with human enteric disease. experiments using control strains showed that the realtime pcr assay was specific and reproducible, with a detection level of 1 colony-forming unit (cfu)/g. the assay was then applied to determine contamination rates in 30 samples of three types of chicken-meat products obtained from five retail outlets in spain (valencia ... | 2008 | 18843599 |
| helicobacter species and common gut bacterial dna in gallbladder with cholecystitis. | to analyze the association between helicobacter spp. and some common gut bacteria in patients with cholecystitis. | 2010 | 20939110 |
| could helicobacter organisms cause inflammatory bowel disease? | the discovery of helicobacter pylori sparked a revolution in the understanding and management of peptic ulcer disease and gastric cancer. other helicobacter species are recognized as important pathogenic agents in colitic diseases of rodents and primates, in particular helicobacter bilis, helicobacter fennelliae, helicobacter hepaticus and helicobacter trogontum. helicobacter bilis and h. hepaticus are now routinely used to initiate rodent models of inflammatory bowel disease (ibd), particularly ... | 2010 | 20955468 |
| characterization of n-linked protein glycosylation in helicobacter pullorum. | the first bacterial n-linked glycosylation system was discovered in campylobacter jejuni, and the key enzyme involved in the coupling of glycan to asparagine residues within the acceptor sequon of the glycoprotein is the oligosaccharyltransferase pglb. emerging genome sequence data have revealed that pglb orthologues are present in a subset of species from the deltaproteobacteria and epsilonproteobacteria, including three helicobacter species: h. pullorum, h. canadensis, and h. winghamensis. in ... | 2010 | 20581208 |
| helicobacter pullorum outbreak in c57bl/6ntac and c3h/hentac barrier-maintained mice. | helicobacter pullorum is a bacterial pathogen in humans. by using microaerobic culture techniques, h. pullorum was isolated from the feces of barrier-maintained mice and identified, on the basis of biochemical, restriction fragment length polymorphism, and 16s rrna gene sequence analyses. this finding presents an opportunity to study h. pullorum pathogenesis in mice. | 2010 | 20220161 |
| bacteremia caused by a novel helicobacter species in a 28-year-old man with x-linked agammaglobulinemia. | we report on a case of bacteremia caused by a previously unknown urease-negative helicobacter strain, immib hp-28/08, isolated from blood cultures of a 28-year-old man with x-linked agammaglobulinemia. the identification of the isolate was based on 16s rrna gene sequencing. in the phylogenetic tree, the isolate fell into a cluster which included helicobacter canadensis, helicobacter equorum, and helicobacter pullorum. this is the first report of bacteremia caused by this fastidious organism. fur ... | 2010 | 20881163 |
| [diarrhea associated with helicobacter pullorum in a child]. | | 2011 | 21412671 |
| [antimicrobial sensitivity of hippurate-negative campylobacter and helicobacter pullorum strains isolated from patients with diarrhea]. | c. jejuni as well as some hippurate-negative campylobacter species and related diarrheagenic organisms, are the leading cause of gastroenteritis in our environment all throughout the year. the aim of the present study was to determine the sensitivity of hippurate-negative campylobacter and helicobacter pullorum strains isolated from the stools of patients with diarrhea. we tested 39 campylobacter coli, two c. lari and five helicobacter pullorum strains identified by mass spectrometry analysis. t ... | 2011 | 22173192 |
| helicobacter species and gut bacterial dna in meckel's diverticulum and the appendix. | to analyse the possible association of various helicobacter species and certain common gut bacteria in patients with meckel's diverticulum and appendicitis. | 2011 | 22039324 |
| resistance of sprague-dawley rats to infection with helicobacter pullorum. | helicobacter pullorum is an enterohepatic helicobacter spp. known to infect both chickens and humans. h. pullorum infection has recently been reported in both mice and rats. this study was designed to determine whether standard methods of colony health surveillance using exposure to rodent soiled bedding could detect h. pullorum in sprague-dawley rats. we exposed 8 helicobacter-free sprague-dawley rats to bedding from h. pullorum-infected brown norway rats. fecal samples were analyzed by pcr eve ... | 2012 | 23294887 |
| chromosomal integration vectors allowing flexible expression of foreign genes in campylobacter jejuni. | campylobacter jejuni is a major cause of human gastroenteritis yet there is limited knowledge of how disease is caused. molecular genetic approaches are vital for research into the virulence mechanisms of this important pathogen. vectors that allow expression of genes in c. jejuni via recombination onto the chromosome are particularly useful for genetic complementation of insertional knockout mutants and more generally for expression of genes in particular c. jejuni host backgrounds. | 2015 | 26497958 |
| prevalence of helicobacter pullorum in conventional, organic, and free-range broilers and typing of isolates. | helicobacter pullorum represents a potential food-borne pathogen, and avian species appear to be a relevant reservoir of this organism. in this study, the prevalence of h. pullorum was investigated at 30 conventional farms where 169 ceca from 34 flocks were tested, at eight organic farms where 39 ceca from eight flocks were tested, and at seven free-range farms where 40 ceca from eight flocks were tested. all of the ceca were obtained from healthy broiler chickens. moreover, amplified fragment l ... | 2010 | 21097592 |
| occurrence of helicobacter pullorum in turkeys. | in order to investigate the occurrence of helicobacter pullorum in turkeys, caecum contents collected at the slaughterhouse from 55 animals intensively reared in 11 farms were sampled. gram-negative curved rod bacteria were isolated by a modified steele and mcdermott filter technique and further identified as h. pullorum by polymerase chain reaction (pcr). eleven and 31 isolates, randomly selected from each positive farm, underwent phenotypic (biochemical and antibiotic susceptibility tests) and ... | 2010 | 21144678 |
| extensive microbial and functional diversity within the chicken cecal microbiome. | chickens are major source of food and protein worldwide. feed conversion and the health of chickens relies on the largely unexplored complex microbial community that inhabits the chicken gut, including the ceca. we have carried out deep microbial community profiling of the microbiota in twenty cecal samples via 16s rrna gene sequences and an in-depth metagenomics analysis of a single cecal microbiota. we recovered 699 phylotypes, over half of which appear to represent previously unknown species. ... | 2014 | 24657972 |
| helicobacter pullorum isolated from fresh chicken meat: antibiotic resistance and genomic traits of an emerging foodborne pathogen. | meat and meat products are important sources of human intestinal infections. we report the isolation of helicobacter pullorum strains from chicken meat. bacteria were isolated from 4 of the 17 analyzed fresh chicken meat samples, using a membrane filter method. mic determination revealed that the four strains showed acquired resistance to ciprofloxacin; one was also resistant to erythromycin, and another one was resistant to tetracycline. whole-genome sequencing of the four strains and comparati ... | 2015 | 26386065 |
| a rapid and efficient luminescence-based method for assaying phosphoglycosyltransferase enzymes. | phosphoglycosyltransferases (pgts) are families of integral membrane proteins with intriguingly diverse architectures. these enzymes function to initiate many important biosynthetic pathways including those leading to peptidoglycan, n-linked glycoproteins and lipopolysaccharide o-antigen. in spite of tremendous efforts, characterization of these enzymes remains a challenge not only due to the inherent difficulties associated with the purification of integral membrane proteins but also due to the ... | 2016 | 27624811 |
| biochar, bentonite and zeolite supplemented feeding of layer chickens alters intestinal microbiota and reduces campylobacter load. | a range of feed supplements, including antibiotics, have been commonly used in poultry production to improve health and productivity. alternative methods are needed to suppress pathogen loads and maintain productivity. as an alternative to antibiotics use, we investigated the ability of biochar, bentonite and zeolite as separate 4% feed additives, to selectively remove pathogens without reducing microbial richness and diversity in the gut. neither biochar, bentonite nor zeolite made any signific ... | 2016 | 27116607 |
| natural and experimental helicobacter pullorum infection in brown norway rats. | helicobacter pullorum is an enterohepatic helicobacter species (ehs) that was recently reported as a naturally acquired infection in mice. faecal samples from 18 out of 20 brown norway (bn) rats, housed in the same barrier as the h. pullorum-infected mice, were positive for h. pullorum using species-specific pcr. in addition, we determined whether h. pullorum was able to persistently colonize the gastrointestinal tract and/or biliary tree and elicit tissue inflammation as well as a serum igg res ... | 2012 | 22580914 |
| low occurrence of helicobacter dna in tropical wild birds, venezuela. | enteric helicobacter species (helicobacter pullorum, helicobacter pametensis, helicobacter canadensis, helicobacter anseris, and helicobacter brantae) have been found in birds from temperate latitudes. we evaluated the occurrence of helicobacter spp. in terrestrial wild birds from venezuela. a fragment of 16s rrna gene was amplified by pcr with helicobacter genus-specific primers. helicobacter spp. were detected in four of 80 fecal and in three of 42 intestinal tissue samples. analyses of 16s an ... | 2013 | 24502727 |
| gastric and enterohepatic non-helicobacter pylori helicobacters. | a substantial number of reports published in the last year have contributed to a better understanding of both human and animal infection with non-helicobacter pylori helicobacter species (nhph). gastric infection of humans with helicobacter suis and helicobacter felis as well as unidentified nhph has been described to cause a chronic gastritis and a variety of clinical symptoms, whereas enterohepatic nhph, including helicobacter cinaedi, helicobacter bilis, and helicobacter canis, have been repo ... | 2013 | 24011248 |
| description of helicobacter valdiviensis sp. nov., an epsilonproteobacteria isolated from wild bird faecal samples. | two gram-stain-negative, gently curved rod-shaped isolates (wbe14(t) and wbe19), recovered from wild bird faecal samples in the city of valdivia (southern chile) were subjected to a polyphasic taxonomic study. results of a genus-specific pcr indicated that these isolates belonged to the genus helicobacter. this was further confirmed by a phylogenetic analyses based on the 16s rrna, 60 kda heat-shock protein (cpn60) and gyrase subunit b (gyrb) genes, where both strains formed a novel phylogenetic ... | 2014 | 24585375 |
| the cytolethal distending toxin subunit cdtb of helicobacter induces a th17-related and antimicrobial signature in intestinal and hepatic cells in vitro. | enterohepatic helicobacter species are associated with several digestive diseases. helicobacter pullorum is an emerging human foodborne pathogen, and helicobacter hepaticus is a mouse pathogen; both species are associated with intestinal and/or hepatic diseases. they possess virulence factors, such as cytolethal distending toxin (cdt). data indicate that cdt may be involved in chronic inflammatory responses, via its active subunit, cdtb. the proinflammatory properties of the cdtb of h. pullorum ... | 2016 | 26908757 |
| genome dynamics and molecular infection epidemiology of multidrug-resistant helicobacter pullorum isolates obtained from broiler and free-range chickens in india. | some life-threatening, foodborne, and zoonotic infections are transmitted through poultry birds. inappropriate and indiscriminate use of antimicrobials in the livestock industry has led to an increased prevalence of multidrug-resistant bacteria with epidemic potential. here, we present a functional molecular epidemiological analysis entailing the phenotypic and whole-genome sequence-based characterization of 11 h. pullorum isolates from broiler and free-range chickens sampled from retail wet mar ... | 2017 | 27815276 |
| helicobacter pullorum induces nitric oxide release in murine macrophages that promotes phagocytosis and killing. | helicobacter pullorum is an avian enterohepatic species that, more recently, has also been found as a naturally acquired infection in mice and rats, and isolated from patients with gastrointestinal and hepatobiliary diseases. in this work, the interaction between h. pullorum and murine macrophages was examined. firstly, the impact of nitric oxide, which is an antimicrobial produced by mammalian macrophages, on h. pullorum 6350-92 viability and morphology was studied by colony-forming assays and ... | 2016 | 26764024 |
| effects of dietary fiber on cecal short-chain fatty acid and cecal microbiota of broiler and laying-hen chicks. | this experiment was conducted to evaluate the effects of feeding dietary fiber on cecal short-chain fatty acid (scfa) concentration and cecal microbiota of broiler and laying-hen chicks. the lower fiber diet was based on corn-soybean meal (sbm) and the higher fiber diet was formulated using corn-sbm-dried distillers grains with solubles (ddgs) and wheat bran to contain 60.0 g/kg of both ddgs and wheat bran from 1 to 12 d and 80.0 g/kg of both ddgs and wheat bran from 13 to 21 d. diets were formu ... | 2015 | 26316341 |
| helicobacter pullorum cytolethal distending toxin targets vinculin and cortactin and triggers formation of lamellipodia in intestinal epithelial cells. | helicobacter pullorum, a bacterium initially isolated from poultry, has been associated with human digestive disorders. however, the factor responsible for its cytopathogenic effects on epithelial cells has not been formally identified. the cytopathogenic alterations induced by several human and avian h. pullorum strains were investigated on human intestinal epithelial cell lines. moreover, the effects of the cytolethal distending toxin (cdt) were evaluated first by using a wild-type strain and ... | 2014 | 24470577 |
| novel function assignment to a member of the essential hp1043 response regulator family of epsilon-proteobacteria. | hp1043 of helicobacter pylori is an orphan response regulator (rr) with a highly degenerate receiver sequence incapable of phosphorylation, which is essential for cell viability. in contrast, the orthologous rr protein of helicobacter pullorum, an enterohepatic helicobacter species mainly isolated from poultry, harbours a consensus receiver sequence and is associated with a cognate histidine kinase (hk). here, we show that this two-component system of h. pullorum, denoted hpmg439/hpmg440, is inv ... | 2013 | 23475951 |
| the pathogenic potential of helicobacter pullorum: possible role for the type vi secretion system. | helicobacter pullorum is a putative enterohepatic pathogen that has been associated with hepatobiliary and gastrointestinal diseases in chickens and in humans. the pathogenic potential of h. pullorum nctc 12826 was investigated. | 2013 | 23067230 |
| nitrosative stress defences of the enterohepatic pathogenic bacterium helicobacter pullorum. | helicobacter pullorum is an avian bacterium that causes gastroenteritis, intestinal bowel and hepatobiliary diseases in humans. although h. pullorum has been shown to activate the mammalian innate immunity with release of nitric oxide (no), the proteins that afford protection against no and reactive nitrogen species (rns) remain unknown. here several protein candidates of h. pullorum, namely a truncated (trhb) and a single domain haemoglobin (sdhb), and three peroxiredoxin-like proteins (prx1, p ... | 2017 | 28855660 |
| helicobacter pullorum: an emerging zoonotic pathogen. | helicobacter pullorum (h.pullorum) commonly colonizes the gastrointestinal tract of poultry causing gastroenteritis. the bacterium may be transmitted to humans through contaminated meat where it has been associated with colitis and hepatitis. despite the high prevalence of h. pullorum observed in poultry, little is known about the mechanisms by which this bacterium establishes infection in host and its virulence determinants. in this article we aim to provide an overview of this emerging zoonoti ... | 2017 | 28443081 |
| effect of heparin, fucoidan and other polysaccharides on adhesion of enterohepatic helicobacter species to murine macrophages. | helicobacter species have been isolated and cultured from both the gastric and enterohepatic niches of the gastrointestinal tract and are associated with a wide spectrum of diseases. some members of the enterohepatic helicobacter species (ehs), which include helicobacter bilis, helicobacter hepaticus and helicobacter pullorum, are associated with chronic inflammatory and proliferative bowel inflammation, hepatitis and in experimental murine studies with hepatic cancer. the present study aimed to ... | 2010 | 21088929 |
| the interplay between campylobacter and helicobacter species and other gastrointestinal microbiota of commercial broiler chickens. | poultry represent an important source of foodborne enteropathogens, in particular thermophilic campylobacter species. many of these organisms colonize the intestinal tract of broiler chickens as harmless commensals, and therefore, often remain undetected prior to slaughter. the exact reasons for the lack of clinical disease are unknown, but analysis of the gastrointestinal microbiota of broiler chickens may improve our understanding of the microbial interactions with the host. | 2014 | 24940386 |
| persistent helicobacter pullorum colonization in c57bl/6ntac mice: a new mouse model for an emerging zoonosis. | helicobacter pullorum, an enterohepatic helicobacter species, is associated with gastroenteritis and hepatobiliary disease in humans and chickens. recently, a novel h. pullorum outbreak in barrier-maintained rats and mice was described. in this study, persistence of infection and serological responses were further evaluated in h. pullorum-infected female c57bl/6ntac and c3h/hentac mice obtained from the barrier outbreak. c57bl/6ntac mice (n=36) aged 10-58 weeks were confirmed to be chronically i ... | 2012 | 22301616 |