| massive occurrence of rickettsiae of the spotted fever group in fowl tampan, argas persicus, in the armenian s.s.r. | fowl tampans (argas persicus) collected in oktemberyan in the armenian s.s.r. in 1974 were found to be massively infected with rickettsiae of the spotted fever (sf) group. one isolated rickettsial strain, designated armenia 9, is antigenically related to, and probably identical with, strain armenia 11 and strain b of rickettsia slovaca, isolated from dermacentor marginatus ticks. strain armenia 9 grows readily in chick embryo yolk sacs and in chick embryo cell (cec) and l-cell cultures. it cause ... | 1977 | 22239 |
| experimental infection of hare (lepus europaeus) with coxiella burnetii and rickettsia slovaca. | hares (lepus europaeus l.) infected subcutaneously (s.c.) or per os with coxiella burnetii and s.c. with rickettsia slovaca overcame an inapparent infection accompanied by irregular rickettsaemia and distribution of rickettsiae to different organs and variable antibody response. neither riskettsia could be detected in faeces of infected animals, but c. burnetii was found in the urine of one hare, which died on day 13 post infection (p.i.). antibodies against c. burnetii persisted for one year of ... | 1978 | 30267 |
| rickettsiae of the spotted fever isolated from dermacentor marginatus ticks in south germany. | rickettsiae of the spotted fever group were detected by haemocyte test in 8 (12%) of 51 partially engorged dermacentor marginatus females collected in march/april 1975 in south germany. four strains of rickettsiae were isolated from ticks positive in haemocyte test. it was concluded with isolated strain brd-1 and homologous immune guinea pig, hamster and mouse sera in cf cross reactions with antigens and immune sera against other members of the sf group, that the newly isolated strains are simil ... | 1977 | 930478 |
| occurrence of coxiella burnetii, rickettsia slovaca, and organisms resembling bacillary rickettsiae in their natural foci in slovakia 20 years after their first detection. | | 1990 | 2227718 |
| rickettsiae in the sumava region. | antibodies were detected to c oxiella burnetii, rickettsia slovaca and chlamydia psittaci in the blood of small terrestrial mammals, larger wild and domestic animals and humans in the southern part of the protected landscape area of the sumava region between 1977 and 1982. the data obtained have contributed to the safe development of livestock raising in this region. | 1985 | 4007702 |
| toxicity of rickettsia slovaca. | | 1981 | 6116420 |
| first report of an infection with rickettsia slovaca. | a description was given of an infection with rickettsia slovaca following a tick bite. the clinical picture was that of an acute meningoencephalitis. its etiology was confirmed serologically by an increase in complement-fixating antibodies, from negative values to diagnostically significant titers. there were febrile relapses and a prolonged persistence of neurasthenic disorders. | 1980 | 6893828 |
| the ultrastructure of rickettsia slovaca in naturally infected females of the tick dermacentor marginatus. | the ultrastructure of rickettsia slovaca in naturally infected females of the tick dermacentor marginatus is similar to that of other members of the spotted fever group rickettsiae. r. slovaca was found to multiply in all organs, tissues and cells examined. it is primarily found free in the cytoplasm; occasionally it may also invade the cell nucleus. special attention was paid to the rickettsiae in the salivary gland. they multiply in all cells of the three acini types i-iii and also in the duct ... | 1980 | 7406420 |
| [rickettsia slovaca: an agent of the group of exanthematous fevers, in portugal]. | identification of rickettsia isolated from dermacentor marginatus ticks from portugal. | 1995 | 7779874 |
| genomic identification of rickettsia slovaca among spotted fever group rickettsia isolates from dermacentor marginatus in armenia. | restriction fragment length polymorphism (rflp) analysis of polymerase chain reaction (pcr) amplified genes was used for genomic identification of armenian isolates of the spotted fever group (sfg) rickettsiae with unclear taxonomic position. analysis was performed by using one genus-specific primer pair derived from r. prowazekii citrate synthase gene and two species-specific primer pairs derived from r. rickettsii genes for 190 k and 120 k antigens following alui, psti and rsai digestion of am ... | 1994 | 7793355 |
| proteinic and genomic identification of spotted fever group rickettsiae isolated in the former ussr. | sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), restriction fragment length polymorphism of polymerase chain reaction-amplified genes (rflp-pcr), and pulsed-field gel electrophoresis (pfge) were used to identify 25 isolates of spotted fever group rickettsia collected in the former ussr. six rickettsia akari isolates which were identical to the mk reference strain from the american type culture collection were found. also, 14 isolates were found to be rickettsia sibirica and ... | 1993 | 7902843 |
| identification of spotted fever group rickettsiae isolated from dermacentor marginatus and ixodes ricinus ticks collected in switzerland. | when 155 ticks collected in different regions of switzerland were tested by the hemolymph test, 10.3% were found to contain spotted fever group rickettsiae. six rickettsial isolates were made from dermacentor marginatus ticks and three were made from ixodes ricinus ticks. the polymerase chain reaction followed by restriction fragment length polymorphism analysis showed that the dermacentor ticks were infected with rickettsia length polymorphism analysis showed that the dermacentor ticks were inf ... | 1994 | 7915498 |
| first isolation of rickettsia slovaca from dermacentor marginatus in france. | three rickettsial strains isolated from dermacentor marginatus ticks in southern france in 1991 were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blotting with polyclonal mouse antisera, microimmunofluorescence serologic typing, and the polymerase chain reaction followed by analysis of restriction fragment length polymorphisms. by these methods, the isolates appear to be identical to the spotted fever group rickettsia, rickettsia slovaca, which has so far b ... | 1993 | 8095380 |
| genetic variation in australian spotted fever group rickettsiae. | rickettsiae were isolated by cell culture of buffy coat blood from six patients with spotted fever from southeastern australia and flinders island in bass strait. the isolates were genetically compared with two previous rickettsia australis patient isolates. the genus-specific 17-kda genes from the isolates were compared after dna amplification and restriction fragment analysis of the amplified dna. this comparison revealed that mainland rickettsial isolates from southeastern australia were iden ... | 1996 | 8735110 |
| citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. | using pcr and an automated laser fluorescent dna sequencer, we amplified and sequenced a 1,234-bp fragment of the citrate synthase-encoding gene (glta) of 28 bacteria belonging to the genus rickettsia. comparative sequence analysis showed that most of the spotted fever group (sfg) rickettsiae belonged to one of two subgroups. the first subgroup included rickettsia massiliae, strain bar 29, rickettsia rhipicephali, "rickettsia aeschlimanni," and rickettsia montana, which have been isolated only f ... | 1997 | 9103608 |
| a new tick-transmitted disease due to rickettsia slovaca. | | 1997 | 9228967 |
| taxonomic relationships among spotted fever group rickettsiae as revealed by antigenic analysis with monoclonal antibodies. | the spotted fever group (sfg) is made up of more than 20 different rickettsial species and strains. study of the taxonomic relationships among the group has been attempted by phenotypic, genotypic, and phylogenetic analyses. in this study, we determined taxonomic relationships among the sfg rickettsiae by comparative analysis of immunogenic epitopes reactive against a panel of monoclonal antibodies. a total of 98 monoclonal antibodies, which were directed against epitopes on the major immunodomi ... | 1998 | 9542904 |
| phylogenetic analysis of spotted fever group rickettsiae by study of the outer surface protein rompa. | rickettsiae are classified in the order rickettsiales and have been included in the alpha subclass of the class proteobacteria on the basis of 16s rrna gene sequence comparison. to estimate the evolutionary forces that have shaped the members of the spotted fever group (sfg) rickettsiae, the ompa gene (apart from the tandem repeat units), encoding an antigenic high-molecular-mass membrane protein specific for the group, was amplified and sequenced from 21 isolates. the phylogenetic relationship ... | 1998 | 9734038 |
| rickettsia slovaca sp. nov., a member of the spotted fever group rickettsiae. | the name rickettsia slovaca sp. nov. (type strain is strain b) is proposed for a member of the spotted fever group (sfg) rickettsiae which was isolated from dermacentor marginatus ticks in slovakia in 1968, and was recently implicated in human febrile illness. this rickettsia can be phenotypically distinguished from other sfg rickettsiae by microimmunofluorescence serotyping, sds-page, western blotting and mabs. genotypic differences between r. slovaca and the other sfg representatives can be de ... | 1998 | 9828449 |
| phylogeny of rickettsia spp. inferred by comparing sequences of 'gene d', which encodes an intracytoplasmic protein. | 'gene d' is the ps120-protein-encoding gene, first described in rickettsia conorii and rickettsia japonica. sequence analysis of a 3030 bp fragment of 'gene d' in 24 representatives of the genus rickettsia was carried out to complete phylogenetic analyses previously inferred by comparison of gene sequences encoding citrate synthase, 17 kda antigen and rompa and rompb. the phylogenetic relationships between rickettsiae were inferred from the comparison of both the gene and the derived protein seq ... | 2001 | 11491333 |
| spotless rickettsiosis caused by rickettsia slovaca and associated with dermacentor ticks. | the pathogenic role of rickettsia slovaca was first demonstrated in 1997 in a patient who presented with a single inoculation lesion of the scalp and enlarged cervical lymph nodes after receiving a bite from a dermacentor tick. subsequently, we evaluated the occurrence of r. slovaca infections among patients living in france and hungary who presented with these symptoms. r. slovaca infections were confirmed by polymerase chain reaction (pcr) in 17 of 67 enrolled patients. infections were most li ... | 2002 | 11981728 |
| tick-borne lymphadenopathy (tibola). | from 1996 through 2000, we collected data on 86 patients with similar symptoms following a tick bite. the inclusion criteria were: enlarged regional lymph nodes and/or vesicular-ulcerative local reaction at the site of the tick bite. | 2002 | 12422619 |
| first isolation of rickettsia slovaca from a patient, france. | | 2003 | 12533298 |
| a case of tick-transmitted lymphadenopathy in bulgaria associated with rickettsia slovaca. | | 2003 | 12751725 |
| prevalence of rickettsia spp. in dermacentor marginatus ticks removed from game pigs (sus scrofa) in southern france. | during the months of october and november 2000 a total of 70 dermacentor marginatus ticks were removed from different game pigs sus scrofa in southern france and investigated by pcr using primers derived from the citrate synthase (glta) and the outer membrane protein (ompb) genes of rickettsiae. based on sequence analysis of 1,150 bp fragment of glta, rickettsia slovaca was identified in 11 ticks (15.7%). these results confirm that rickettsia slovaca, an emerging pathogen is highly prevalent in ... | 2003 | 12860624 |
| epidemiological and clinical differences among rickettsia slovaca rickettsiosis and other tick-borne diseases in spain. | | 2003 | 12860652 |
| serologically detected "new" tick-borne zoonoses in eastern croatia. | to establish serologically a contact with causative agents of human monocytic and granulocytic ehrlichiosis, human babesiosis, recently detected rickettsioses, and lyme disease in individuals with a history of tick bite from three counties in eastern croatia. | 2003 | 14515426 |
| detection and identification of spotted fever group rickettsiae in ticks collected in southern croatia. | a total of 197 ticks belonging to four species (haemaphysalis punctata, hyalomma marginatum, rhipicephalus bursa and dermacentor marginatus) collected in october 2000 from domestic animals in southern croatia were examined for the presence of rickettsiae by molecular techniques. specific sequences of the rickettsiae were detected in 25 (12.7%) of ticks tested. the prevalence of infection in d. marginatus and h. marginatum ticks was 36.8 and 64.7%, respectively. none of the ticks belonging to the ... | 2002 | 14570128 |
| dermacentor-borne necrosis erythema and lymphadenopathy: clinical and epidemiological features of a new tick-borne disease. | this paper describes the epidemiological and clinical features of a tick-borne disease differing somewhat from other tick-borne diseases found previously in spain. all patients were bitten by dermacentor marginatus or a large tick. the clinical features include a crustaceous or necrotic lesion at the site of the tick's attachment, surrounded by an erythema (erythema migrans-like) and painful regional lymphadenopathies. the probable aetiological agent is rickettsia slovaca. similar cases have bee ... | 2004 | 15059122 |
| concomitant or consecutive infection with coxiella burnetii and tickborne diseases. | q fever is a worldwide zoonosis caused by coxiella burnetii, which can be isolated from ticks. reports of people with both q fever and other tickborne diseases are rare. in this study, we describe 6 patients with q fever who were infected with 1 of the following tickborne pathogens: rickettsia conorii (2 patients), rickettsia slovaca (2), rickettsia africae (1), and francisella tularensis (1). | 2004 | 15614696 |
| tick species and tick-borne infections identified in population from a rural area of spain. | to determine the tick species that bite humans in the province of soria (spain) and ascertain the tick-borne pathogens that threaten people's health in that province, 185 tick specimens were collected from 179 patients who sought medical advice at health-care centres. the ticks were identified, and their dna examined by pcr for pathogens. most ticks were collected in autumn and spring (59 and 57 respectively). nine species of ticks were identified, the most frequent being dermacentor marginatus ... | 2005 | 16181517 |
| rickettsia slovaca in dermacentor ticks found on humans in spain. | | 2006 | 16450104 |
| effect of antibiotic treatment in patients with debonel/tibola. | debonel/tibola is a tick-borne acute/sub-acute infection transmitted in our environment by dermacentor marginatus and mainly caused by rickettsia slovaca. the aim of our study was to know the effect of starting early treatment in the course of the debonel/tibola. | 2005 | 16481523 |
| debonel/tibola: is rickettsia slovaca the only etiological agent? | | 2005 | 16481539 |
| detection of spotted fever group (sfg) rickettsiae in dermacentor reticulatus (acari: ixodidae) in poland. | dermacentor reticulatus ticks from poland were investigated by molecular methods for the presence of rickettsiae. during 2003/2004, a total of 285 adult ticks was assayed using primers rpcs.877 and rpcs.1258 derived from the citrate synthase (glta) gene, and 116 samples (40.7%) were positive for rickettsial dna. ten out of these positive samples were further assayed using slo1f and slo1r primers derived form the rompa-encoding gene to confirm that detected rickettsiae belong to the spotted fever ... | 2006 | 16524778 |
| rickettsiae of the spotted-fever group in ixodid ticks from hungary: identification of a new genotype ('candidatus rickettsia kotlanii'). | three common european 'anthrophilic' ticks, ixodes ricinus, haemaphysalis concinna and dermacentor reticulatus, were collected in hungary and tested, in assays based on nested pcr, for rickettsiae of the spotted-fever group. low percentages of i. ricinus (2.7%) and h. concinna (1.0%) and a high percentage of d. reticulatus (26.8%) were found to be infected. the rickettsiae in the ticks were then identified, by sequencing of the genes coding for 16s ribosomal rna (16s rdna), citrate synthase (glt ... | 2006 | 16630380 |
| rickettsia slovaca infection, france. | | 2006 | 16710981 |
| the seroprevalence of human infection with rickettsia slovaca, in an area of northern spain. | an epidemiological survey was undertaken to explore human exposure to rickettsia slovaca in two provinces of northern spain. when ifat were used to test 200 members of the general population for antibodies to rickettsiae of the spotted-fever group, six (3.3%) were found positive, presumably, since dermacentor is one of the most common genera of human-biting tick in the study area, for antibodies to r. slovaca. thirty-one (16.9%) of an additional 183 subjects who presented shortly after being bit ... | 2006 | 16762114 |
| [rickettsia slovaca infection alter a tick bite]. | | 2006 | 17020735 |
| ticks and tick-borne rickettsiae surveillance in montesinho natural park, portugal. | this study constitutes the first contribution to the knowledge of tick dynamics and its implication in the epidemiology of rickettsial diseases in montesinho natural park (mnp), bragança district of portugal. of 76 ticks collected, 12 (15.8%) were dermacentor (d.) marginatus, 36 (47.4%) d. reticulatus, and 28 (36.8%) rhipicephalus (r.) sanguineus. isolation assays were performed by shell-vial technique on 41 ticks. israeli spotted fever strain was an isolate from r. sanguineus, and three isolate ... | 2006 | 17114695 |
| rickettsia slovaca infection: debonel/tibola. | this study describes the epidemiological, clinical, and microbiological characteristics of a new tick-borne disease in spain-dermacentor-borne necrosis erythema lymphadenopathy (debonel). the clinical presentations include an eschar at the site of the tick bite, surrounded by an erythema and painful regional lymphadenopathy. the disease appears during the colder months and its vector is dermacentor marginatus (d. marginatus). from january 1990 to december 2004, 54 patients presented at hospital ... | 2006 | 17114711 |
| prevalence of spotted fever group rickettsia species detected in ticks in la rioja, spain. | our objective was to learn the prevalence of spotted fever group (sfg) rickettsia detected in ticks in la rioja, in the north of spain. from 2001 to 2005, 496 ticks representing 7 tick species were analysed at the hospital de la rioja. ticks were removed from humans with or without rickettsial syndrome (n = 59) or collected from mammals (n = 371) or from vegetation by dragging (n = 66). the presence of sfg rickettsia in these ticks was investigated by semi-nested pcr (ompa gene) and sequencing. ... | 2006 | 17114730 |
| prevalence of rickettsia slovaca in dermacentor marginatus ticks removed from wild boar (sus scrofa) in northeastern spain. | rickettsia slovaca, the causative agent of tibola, is transmitted by dermacentor ticks. dermacentor marginatus is the most widely species distributed in northeastern spain, and the wild boar constitutes the main host. d. marginatus ticks were collected from hunter-killed wild boar and were tested by pcr/rflp. rickettsial dna-positive ticks were sequenced using the ompa pcr primers. the prevalence of r. slovaca in d. marginatus ticks was 17.7%. other spotted fever group rickettsiae were detected ... | 2006 | 17114731 |
| prevalence data of rickettsia slovaca and other sfg rickettsiae species in dermacentor marginatus in the southeastern iberian peninsula. | in southern spain, dermacentor marginatus ticks can be infected with several genospecies of spotted fever group (sfg) rickettsia. we developed a nested polymerase chain reaction assay by using a species-specific probe targeting the ompa gene to detect and differentiate between the two groups of rickettsiae previously described in d. marginatus. sfg rickettsia has been detected in 85.15% of ticks studied (26.7% of positives have been to r. slovaca, the causative agent of tibola-debonel, and 73.3% ... | 2006 | 17114732 |
| spotted fever group rickettsiae in ticks feeding on humans in northwestern spain: is rickettsia conorii vanishing? | during a 7-year study, we identified and analyzed by pcr 4,049 ticks removed from 3,685 asymptomatic patients in castilla y león (northwestern spain). a total of 320 ticks (belonging to 10 species) were pcr-positive for rickettsiae. comparison of amplicon sequences in databases enabled us to identify eight different spotted fever group (sfg) rickettsiae: rickettsia slovaca, rickettsia sp. irs3/irs4, r. massiliae/bar29, r. aeschlimannii, rickettsia sp. rpa4/dns14, r. helvetica, rickettsia sp. dms ... | 2006 | 17114733 |
| evaluation of igg antibody response against rickettsia conorii and rickettsia slovaca in patients with debonel/tibola. | the aim of the study was to determine the igg antibody response to spotted fever group rickettsia (sfgr) r. conorii and r. slovaca, and its specificity and sensitivity in patients with debonel/tibola. a prospective study of 31 patients with debonel was carried out from january 2001 to may 2004. the sfgr serology testing (igg ifa) for the diagnosis of debonel/tibola showed 61% sensitivity and 100% specificity. the r. slovaca antigen allowed the diagnoses in 18 of the 31 patients (58%), and 17 pat ... | 2006 | 17114780 |
| spotted fever rickettsioses in southern and eastern europe. | mediterranean spotted fever due to rickettsia conorii conorii was thought, for many years, to be the only tick-borne rickettsial disease prevalent in southern and eastern europe. however, in recent years, six more species or subspecies within the spotted fever group of the genus rickettsia have been described as emerging pathogens in this part of the world. tick-borne agents include rickettsia conorii israelensis, rickettsia conorii caspia, rickettsia aeschlimannii, rickettsia slovaca, rickettsi ... | 2007 | 17266709 |
| the role of wild boar (sus scrofa) in the eco-epidemiology of r. slovaca in northeastern spain. | rickettsia slovaca is considered the etiological agent of tick-borne lymphadenopathy (tibola), an emerging disease transmitted by tick bites. dermacentor marginatus constitutes the most important vector and wild boar (sus scrofa) is the main wild host in our area. the epidemiology of this tick-borne rickettsioses has not been completely clarified. during hunting season 2004, wild boar sera sample were collected from northeastern spain. ticks were collected both from wild boar and from flagging v ... | 2007 | 17417958 |
| rickettsiae and rickettsial infections: the current state of knowledge. | new human rickettsial pathogens have been discovered, and long-known rickettsiae of undetermined pathogenicity have been demonstrated to cause illness. disease associated with rickettsia slovaca has unique clinical manifestations, including prominent lymphadenopathy without fever and rash. rickettsial genomes are highly conserved, with reductive evolution leading to a small genome that relies on the host cell for many biosynthetic functions. advances in the evaluation of the pathogenesis of rick ... | 2007 | 17582568 |
| rickettsia sp. strain rpa4 detected in portuguese dermacentor marginatus ticks. | dermacentor marginatus species is the common vector of rickettsia slovaca. recently, a new rickettsia genotype, rpa4, was detected in several dermacentor sp. ticks in several countries of the former soviet union and in spain. in an epidemiological surveillance study, ninety-eight d. marginatus, collected in the south of portugal in spring of 2003 and 2004, were screened by polymerase chain reaction (pcr) to detect rickettsiae. overall, 59.2% of the ticks were pcr-positive, among them 34.5% were ... | 2007 | 17627441 |
| rickettsia slovaca in dermacentor marginatus and tick-borne lymphadenopathy, tuscany, italy. | of 263 patients in tuscany, italy, from whom ticks were removed during july 2005-may 2007, five showed signs of tick-borne encephalopathy. of the ticks, 17 were dermacentor marginatus; 6 (35.3%) of these were identified by sequence analysis as containing rickettsia slovaca. tick-borne lympadenopathy occurs in this area. | 2008 | 18439371 |
| rickettsia slovaca infection in humans in the northeast of spain: seroprevalence study. | catalonia is an endemic area of mediterranean spotted fever. in 1997, a. lakos described a new tick-borne infectious disease called tick-borne lymphadenopathy. the causative agent is rickettsia slovaca, which is transmitted by dermacentor marginatus ticks. we have diagnosed human cases in catalonia. the objective of this study was to determinate seroprevalence of r. slovaca infection in humans in the northeast of spain. the population included 217 subjects from catalonia, northeast of spain and ... | 2008 | 18620514 |
| isolation and identification of a novel spotted fever group rickettsia, strain ig-1, from ixodes granulatus ticks collected on orchid island (lanyu), taiwan. | a novel species of spotted fever group (sfg) rickettsia, rickettsia spp. ig-1, was identified and isolated from adult ixodes granulatus ticks collected from orchid island (lanyu), an islet located in southeastern taiwan. serum samples collected from the rodent host rattus losea and sfg-positive human serum reacted with ig-1 using an immunofluorescence assay (ifa). pairwise nucleotide sequence analysis of 16s rrna (rrs), glta, ompa, ompb, and sca4 shows that ig-1 belongs to sfg rickettsiae and ha ... | 2008 | 18689633 |
| specific detection of rickettsia slovaca by restriction fragment length polymorphism of sca4 gene. | | 2008 | 18999896 |
| rickettsia helvetica in dermacentor reticulatus ticks. | we report on the molecular evidence that dermacentor reticulatus ticks in croatia are infected with rickettsia helvetica (10%) or rickettsia slovaca (2%) or co-infected with both species (1%). these findings expand the knowledge of the geographic distribution of r. helvetica and d. reticulatus ticks. | 2009 | 19116063 |
| life cycle of rickettsia slovaca in l929 cell line studied by quantitative real-time pcr and transmission electron microscopy. | rickettsia slovaca, a member of spotted fever rickettsiae, is an agent of a mild human disease known as tibola or debonel. using quantitative real-time pcr we identified the highest point of multiplication of wild and standard type (strain b) of r. slovaca on the second vs. the fourth day postinfection. comparing both types of r. slovaca by transmission electron microscopy substantiated different cytopathological and morphological changes in infected cells and other differences, for example a sl ... | 2009 | 19220472 |
| ultrastructural study of the life cycle of rickettsia slovaca, wild and standard type, cultivated in l929 and vero cell lines. | ultrastructural changes induced by rickettsia slovaca standard type (st) and wild type (wt) were examined during their life cycle in l929 and vero cells. r. slovaca invaded the cytoplasm of the host cell by phagocytosis on the 1st d p.i. rickettsiae adhering to the cytoplasmic membrane were engulfed by cellular extensions and occurred in phagocytic vacuoles. binary fission of rickettsia was observed. the nuclear chromatin of eukaryotic cells was rearranged and condensed during 3rd and 6th d p.i. ... | 2009 | 19418250 |
| typhus and other rickettsioses: emerging infections in germany. | rickettsioses are diseases caused by rickettsiae, obligate intracellular bacteria that are transmitted by arthropods to humans. they cause various types of spotted fever and typhus. | 2009 | 19547738 |
| first detection of rickettsia slovaca in greece. | seven hundred and three ticks collected in november and june of 2004-2006 from domestic animals in four localities of halkidiki prefecture, northern greece, were tested for the presence of ricketsial dna. rickettsia slovaca was detected in one pool of rhipicephalus bursa ticks, representing the first report of detection of r. slovaca in greece. | 2010 | 19554462 |
| rickettsia slovaca and r. raoultii in tick-borne rickettsioses. | tick-borne lymphadenopathy (tibola), also called dermacentor-borne necrosis erythema and lymphadenopathy (debonel), is defined as the association of a tick bite, an inoculation eschar on the scalp, and cervical adenopathies. we identified the etiologic agent for 65% of 86 patients with tibola/debonel as either rickettsia slovaca (49/86, 57%) or r. raoultii (7/86, 8%). | 2009 | 19624931 |
| dermacentor marginatus and ixodes ricinus ticks versus l929 and vero cell lines in rickettsia slovaca life cycle evaluated by quantitative real time pcr. | ticks transmit many different pathogens to animals, humans and their pets. rickettsia slovaca, as a member of the spotted-fever-group rickettsiae is an agent of the human disease tick-borne lymphadenopathy (tibola), also called dermacentor-borne necrosis erythema and lymphadenopathy (debonel), which occurs from the mediterranean to central europe, transmitted by dermacentor reticulatus and dermacentor marginatus (acari: ixodidae). in this study, quantitative real time pcr was used to characteriz ... | 2010 | 19779862 |
| emergence of rickettsia slovaca infection in brittany, france. | | 2009 | 19793131 |
| rickettsia slovaca and rickettsia raoultii in dermacentor marginatus ticks collected on wild boars in tuscany, italy. | during the hunting season 2007-2008, 494 dermacentor marginatus (sulzer) ticks were collected from 109 hunter-killed wild boars, sus scrofa, in lucca's province, tuscany, italy. rickettsia slovaca, the causative agent of tick-borne lymphadenopathy (tibola), was detected in 32.1% of ticks tested (n=112) by using polymerase chain reaction primers targeting glta, ompa, and ompb rickettsial genes. moreover, rickettsia raoultii was found for the first time in italy, with 1.8% infection prevalence. th ... | 2009 | 19960702 |
| rickettsia slovaca in dermacentor marginatus ticks, germany. | | 2009 | 19961713 |
| scalp eschar and neck lymphadenopathy caused by bartonella henselae after tick bite. | rickettsia slovaca and rickettsia raoultii have been associated with a syndrome characterized by scalp eschar and neck lymphadenopathy following tick bites. however, in many cases, the causative agent remains undetermined. we report 3 cases of this syndrome caused by bartonella henselae, and we propose the term "senlat" to collectively describe this clinical entity. | 2010 | 20070235 |
| static and dynamic systems in rickettsia slovaca life cycle evaluated by quantitative real-time polymerase chain reaction. | quantitative real-time polymerase chain reaction was used to characterize the growth of rickettsia slovaca, a tick-borne pathogen transmitted by dermacentor reticulatus and d. marginatus ticks, in static (l929 and vero cells) and dynamic (d. marginatus and ixodes ricinus ticks) cultivation systems. the highest points of bacterial multiplication and the time-spans between the inoculum and the maximum of rickettsial copies were increased in consecutive order from eukaryotic cells, i. ricinus to d. ... | 2010 | 20537110 |
| eschar and neck lymphadenopathy caused by francisella tularensis after a tick bite: a case report. | | 2011 | 21418587 |
| tick-borne lymphadenopathy (tibola) acquired in southwestern germany. | tick-borne lymphadenopathy (tibola) was first described in 1997 in a patient in france. the causative agent, rickettsia slovaca, is transmitted by dermacentor ticks. | 2011 | 21663601 |
| coinfections of rickettsia slovaca and rickettsia helvetica with borrelia lusitaniae in ticks collected in a safari park, portugal. | borrelia and rickettsia bacteria are the most important tick-borne agents causing disease in portugal. identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. a total of 677 questing ticks including dermacentor marginatus, rhipicephalus sanguineus, ixodes ricinus, hyalomma lusitanicum, h. marginatum, and haemaphysalis punctata we ... | 2010 | 21771525 |
| Evaluation of clinical specimens for Rickettsia, Bartonella, Borrelia, Coxiella, Anaplasma, Franciscella and Diplorickettsia positivity using serological and molecular biology methods. | We monitored clinical samples from patients of different age groups from selected regions in Slovakia. Overall seroprevalence evaluated by immunofluorescence (IFA) using nine Bartonella, two Borrelia, six rickettsial (spotted fever and typhus group), two Coxiella, and one human granulocytic ehrlichiosis Anaplasma, Franciscella tularensis and Diplorickettsia massiliensis antigens, in rural and city populations of Slovak Republic, was found to be 32% positive for spotted fever group rickettsiae. O ... | 2011 | 22098390 |
| widespread use of real-time pcr for rickettsial diagnosis. | we report 2 years of experience with rickettsial molecular diagnosis using real-time pcr at the french national reference center. all rickettsia genomes available were compared to discover specific sequences to design new sets of primers and probes. the specificity was verified in silico and against a panel of 30 rickettsial species. sensitivity was determined using 10-fold serial dilutions. finally, primers and probes that were both specific and sensitive were routinely used for the diagnosis o ... | 2011 | 22092999 |
| first report on the occurrence of rickettsia slovaca and rickettsia raoultii in dermacentor silvarum in china. | abstract: background: rickettsioses are among both the longest known and most recently recognized infectious diseases. although new spotted fever group rickettsiae have been isolated in many parts of the world including china, little is known about the epidemiology of rickettsia pathogens in ticks from xinjiang autonomous region of china. methods: in an attempt to assess the potential risk of rickettsial infection after exposure to ticks in xinjiang uygur autonomous region of china, a total of ... | 2012 | 22257726 |
| [case of tibola/debonel (tick - borne lymphadenopathy/dermacentor spp. - borne necrosis - erythema - lymphadenopathy) in poland]. | rickettsia slovaca was recognized as the causative agent of the disease named tick - borne lymphadenopathy (tibola) or dermacentor spp.- borne necrosis - erythema lymphadenopathy (debonel). generally, this ricketsiosis is considered a mild disease but the disease is considered a mild rickettsiosis, but severe symptoms can appeare, especially in untreated patients. described infection is the first case of tibola/debonel found in poland. the patient had typical symptoms, such as enlarged painful c ... | 2011 | 22390043 |
| rickettsial diseases. | rickettsiae are a rather diverse collection of organisms with several differences; this prohibits their description as a single homogenous group. rickettsiae are maintained in nature through a cycle involving reservoir in mammals and arthropod vectors. the public health impact of these on lives or productivity lost is largely unmeasured, but suspected to be quite high worldwide. the diseases caused by rickettsia and orientia species are often collectively referred to as rickettsioses. coxiella b ... | 2012 | 23405539 |
| detection of bartonella tamiae, coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern algeria. | in recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. in algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. for this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in el tarf and souk ahras, algeria. | 2016 | 26791781 |
| assessment of domestic goats as models for experimental and natural infection with the north american isolate of rickettsia slovaca. | rickettsia slovaca is a tick-borne human pathogen that is associated with scalp eschars and neck lymphadenopathy known as tick-borne lymphadenopathy (tibola) or dermacentor-borne necrosis erythema and lymphadenopathy (debonel). originally, r. slovaca was described in eastern europe, but since recognition of its pathogenicity, human cases have been reported throughout europe. european vertebrate reservoirs of r. slovaca remain unknown, but feral swine and domestic goats have been found infected o ... | 2016 | 27741322 |
| first report of rickettsia raoultii and r. slovaca in melophagus ovinus, the sheep ked. | melophagus ovinus (diptera: hippoboscidae), a hematophagous ectoparasite, is mainly found in europe, northwestern africa, and asia. this wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. herein, we investigated the presence of various rickettsia species in m. ovinus. | 2016 | 27884170 |
| rickettsia slovaca infection in humans, portugal. | fifteen years after the initial detection of rickettsia slovaca in ticks in portugal, 3 autochthonous cases of r. slovaca infection were diagnosed in humans. all patients had an eschar on the scalp and lymphadenopathy; 2 patients had facial edema. r. slovaca infection was confirmed by serologic testing, culture, and pcr. | 0 | 24050379 |
| evaluation of the presence of rickettsia slovaca infection in domestic ruminants in catalonia, northeastern spain. | rickettsia slovaca is the etiological agent of the human disease tick-borne lymphadenopathy (tibola) transmitted by dermacentor spp. ticks. in our area, dermacentor marginatus is the most important tick vector; adult ticks feed on mammals, especially ungulates such as wild boars and domestic ruminants. the epidemiology of tick-transmitted diseases describes a wild cycle and a domestic cycle and both are connected by ticks. to identify the role of domestic ruminants in the transmission and mainte ... | 2012 | 23186170 |
| complete genome sequence of rickettsia slovaca, the agent of tick-borne lymphadenitis. | the present study reports the complete and annotated genome sequence of the human pathogen rickettsia slovaca strain 13-b, which was isolated from a dermacentor tick in slovakia in 1968. the 1.27-mb genome provides further insights into the acquisition of virulence related to genome reduction in rickettsia species. | 0 | 22374949 |
| molecular evidence for bacterial and protozoan pathogens in hard ticks from romania. | the aim of the present study was to provide a preliminary insight into the diversity of tick-borne pathogens circulating at the domestic host-tick interface in romania. for this, feeding and questing ticks were analyzed by real-time polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum, anaplasma platys, ehrlichia canis, borrelia burgdorferi sensu latu, and by pcr and subsequent sequencing for rickettsia spp., babesia spp. and theileria spp. a total of 382 ticks, encompas ... | 2013 | 23428204 |
| molecular detection of rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, romania. | the diverse tick fauna as well as the abundance of tick populations in romania represent potential risks for both human and animal health. spotted fever group (sfg) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. however, the epidemiology of rickettsial diseases has been poorly investigated in romania. in urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservo ... | 2016 | 26507182 |
| sympatric occurrence of ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks and rickettsia and babesia species in slovakia. | vojka nad dunajom in the south-west of the slovak republic is a locality with sympatric occurrence of 3 species of ticks. this study investigated the spatial distribution of dermacentor reticulatus, ixodes ricinus, and haemaphysalis concinna ticks in this area and determined the prevalence of babesia and rickettsia species in questing adults of these tick species considered as potential risk for humans and animals. ticks were collected by blanket dragging over the vegetation from september 2011 ... | 2014 | 24973275 |
| antibodies to rickettsia spp. and borrelia burgdorferi in spanish wild red foxes (vulpes vulpes). | we examined 314 red foxes (vulpes vulpes) from the province of soria, spain, for rickettsia typhi, rickettsia slovaca, and borrelia burgdorferi infection. immunofluorescence assays showed 1.9% had antibodies to r. typhi, 6.7% had antibodies to r. slovaca, and 8.3% had antibodies to b. burgdorferi. serostatus was not correlated with sex or age. because red foxes can be infected by rickettsiae and b. burgdorferi, presence of red foxes may be and indicator for the presence of these pathogens. | 2016 | 26540334 |
| scalp eschar and neck lymphadenopathy after tick bite: an emerging syndrome with multiple causes. | the clinical and epidemiological features of 56 patients with scalp eschar associated with neck lymphadenopathy after a tick bite (senlat) syndrome were evaluated at the national french rickettsial center. eschar swabs, crusts, and biopsies as well as ticks and blood samples were acquired for molecular and serological assays. senlat predominantly affects children (p < 0.05), followed by 40- to 70-year-olds, and it is found mostly in women (p < 0.05). the seasonal distribution has two peaks: one ... | 2014 | 24682865 |
| molecular evidence of rickettsia spp. in ixodid ticks and rodents in suburban, natural and rural habitats in slovakia. | natural foci of tick-borne spotted fever group (sfg) rickettsiae of public health concern have been found in slovakia, but the role of rodents in their circulation is unclear. ticks (ixodes ricinus, ixodes trianguliceps, dermacentor marginatus, dermacentor reticulatus, haemaphysalis concinna and haemaphysalis inermis) and tissues of rodents (apodemus flavicollis, apodemus sylvaticus, myodes glareolus, microtus arvalis, microtus subterraneus and micromys minutus) were examined for the presence of ... | 2017 | 28340608 |
| detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy. | tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ... | 2017 | 28110915 |
| rickettsiae and rickettsial diseases in croatia: implications for travel medicine. | to review the current state of knowledge concerning rickettsiae and rickettsioses in croatia and to discuss their implications for travellers. | 2017 | 27404664 |
| molecular detection of spotted fever group rickettsiae in ticks removed from humans in turkey. | a total of 229 attached hard ticks were collected from humans in yozgat province (turkey) in 2009. all ticks were individually examined for the presence of spotted fever group (sfg) rickettsiae using the polymerase chain reaction (pcr) targeting rickettsial citrate synthase (glta) and outer membrane protein (ompa) genes. rickettsial dna was detected in 24 (10.5%) tick samples belonging to dermacentor marginatus (sulzer) (9 males, 3 females), hyalomma marginatum koch (8 males, 2 females) and haem ... | 2016 | 27131413 |
| rickettsioses in europe. | bacteria of the genera rickettsia and orientia (family rickettsiaceae, order rickettsiales) cause rickettsioses worldwide, and are transmitted by lice, fleas, ticks and mites. in europe, only rickettsia spp. cause rickettsioses. with improvement of hygiene, the risk of louse-borne rickettsiosis (epidemic typhus) is low in europe. nevertheless, recrudescent form of rickettsia prowazekii infection persists. there could be an epidemic typhus outbreak if a body lice epidemic occurs under unfavorable ... | 2016 | 26384814 |
| development of three quantitative real-time pcr assays for the detection of rickettsia raoultii, rickettsia slovaca, and rickettsia aeschlimannii and their validation with ticks from the country of georgia and the republic of azerbaijan. | a previous surveillance study of human pathogens within ticks collected in the country of georgia showed a relatively high infection rate for rickettsia raoultii, r. slovaca, and r. aeschlimannii. these 3 spotted fever group rickettsiae are human pathogens: r. raoultii and r. slovaca cause tick-borne lymphadenopathy (tibola), and r. aeschlimannii causes an infection characterized by fever and maculopapular rash. three quantitative real-time polymerase chain reaction (qpcr) assays, rraoul, rslov, ... | 2012 | 23182543 |
| tick-borne rickettsioses in europe. | rickettsioses are caused by obligate intracellular bacteria within the genus rickettsia, mainly transmitted by arthropods. until recently, mediterranean spotted fever (msf) caused by rickettsia conorii was considered the only tick-borne rickettsiosis in europe. however, 'new' tbr have been described in europe during last years. for instance, other subspecies such as r. conorii caspia and r. conorii israelensis have been involved in msf. dermacentor-borne necrosis erythema and lymphadenopathy/tic ... | 2012 | 23177355 |
| rickettsia slovaca from dermacentor marginatus ticks in sardinia, italy. | nineteen ticks belonging to the species dermacentor marginatus, rhipicephalus bursa, and haemaphysalis sulcata were collected from wild animals (wild boar, deer, and mouflon) in south-western sardinia, italy. five d. marginatus ticks from wild boar were pcr-positive when analyzed using glta-specific and ompa-specific primers, leading to the identification and first isolation in cell culture of rickettsia slovaca, the causative agent of tick-borne lymphadenopathy (tibola), on the island of sardin ... | 2012 | 23140897 |
| rickettsia massiliae infection and senlat syndrome in romania. | the purpose of this prospective study is to describe the clinical and epidemiological characteristics of rickettsioses in romania, where only rickettsia conorii is known by clinicians but new rickettsia species have been identified recently in ticks. a total of eight patients, including a nine-year-old child, were included between june 2011 and june 2012, in the hospital for infectious and tropical diseases, bucharest, romania. seven cases presented during summer months and one in spring. six pa ... | 2016 | 27034192 |
| bacterial agents in 248 ticks removed from people from 2002 to 2013. | a retrospective study was conducted to analyze the tick species removed from people and to detect tick-infecting bacteria in the specimens collected over the past 10 years at the reference center for rickettsioses, marseille, france. a total of 248 ticks were removed from 200 people, including dermacentor (73), rhipicephalus (67), ixodes (60), amblyomma (8), argas (3), hyalomma (1), and haemaphysalis (1) species. bacterial dna was detected in 101 ticks: rickettsia slovaca (34%) and rickettsia ra ... | 2016 | 26874669 |
| the role of particular tick developmental stages in the circulation of tick-borne pathogens affecting humans in central europe. 3. rickettsiae. | ixodes ricinus, dermacentor reticulatus and d. marginatus ticks are the most important vector for rickettsia spp. in central europe. ticks sustain rickettsial transmission cycles transovarially and transstadially, it makes enable the rickettsial circulation in the tick population in the absence of vertebrate competent reservoir. rickettsia helvetica is transmitted by i. ricinus tick; the highest rates of infection are noted in adult females, lower in males and in nymphs. all tick developmental s ... | 2017 | 27614472 |
| isolation of rickettsia helvetica from ticks in slovakia. | to date, only three rickettsial species have been found in ticks in slovakia by serological and/or molecular-biological techniques, namely rickettsia slovaca, candidatus rickettsia irs, and rickettsia raoultii. recently, we succeeded in isolation of the forth species, rickettsia helvetica from ixodes ricinus, the most frequent tick in slovakia. the isolation, positive for 10% of tested ticks, was performed on xtc cells by the shell-vial technique, gimenez staining and light microscopy. the infec ... | 2012 | 23043605 |
| [rickettsiosis after tick bite: a subtle clinic picture on many occasions, we must be vigilant]. | rickettsia diseases are a group of tick-borne transmitted diseases, classified into 2 large groups: spotted fevers and typhus fevers. in addition, a new condition has been described recently, known as tick-borne lymphadenopathy. a retrospective series is presented of paediatric cases of rickettsia diseases diagnosed in 2013 and 2014. a total of 8 patients were included, of which 2 of them were diagnosed as mediterranean spotted fever, and 6 as tick-borne lymphadenopathy. rickettsia slovaca, rick ... | 2017 | 26976382 |
| comparative value of blood and skin samples for diagnosis of spotted fever group rickettsial infection in model animals. | the definitive diagnosis of spotted fever group (sfg) rickettsioses in humans is challenging due to the retrospective nature and cross reactivity of the serological methods and the absence of reliable and consistent samples for molecular diagnostics. existing data indicate the transient character of bacteremia in experimentally infected animals. the ability of arthropod vectors to acquire rickettsial infection from the laboratory animals in the absence of systemic infection and known tropism of ... | 2016 | 27282078 |
| tick-borne lymphadenopathy, an emerging disease. | tick-borne lymphadenopathy (tibola) is a spotted fever group disease characterized by an eschar and pronounced enlarged lymph nodes after a scalp tick bite. the goal of this synopsis is to review the tibola literature published until may 2013: forty-one articles (reporting 537 cases) were included. there was a predominance of cases in females and young people. spain, france, and hungary reported the majority of cases, and they were mainly reported in the colder seasons. the involved tick bite wa ... | 2014 | 25090977 |