| isolation of spotted fever group rickettsia from apodemus speciosus in an endemic area in japan. | a spotted fever group rickettsia was isolated from apodemus mice captured near the site where a person had been infected in miyazaki prefecture. antigenic characteristics of the isolate designated as strain to-1 were compared with those of rickettsia japonica (strain yh) and rickettsia montana (atcc vr611) by the indirect fluorescent antibody test with 14 serum specimens obtained from apodemus speciosus, immune rat antisera against the strains to-1 and yh, and three patients' sera. the titers of ... | 1992 | 1434069 |
| seroepidemiology of spotted fever group rickettsiae in small field rodents in japan. | to clarify the geographic distribution of spotted fever group (sfg) rickettsiae in japan, small field rodents captured in endemic and nonendemic areas were screened for anti-sfg rickettsia antibodies by the immunofluorescence test. among total 716 specimens tested, 73-75% of rodents were antibody-positive against rickettsia japonica and/or rickettsia montana, showing different degree of antibody-positive proportions among each species of the rodents. interestingly, these profiles were not differ ... | 1992 | 1522815 |
| rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in japan. | we propose the name rickettsia japonica sp. nov. (with type strain yh [= atcc vr-1363]) for a serologically specific species of spotted fever group rickettsiae that are pathogenic for humans (j. infect. dis. 159:1122-1126, 1989; j. clin. microbiol. 28:1177-1180, 1990). the biologic and genomic characteristics of the organism (g+c content, 31.2 +/- 0.7 mol%) are essentially the same as those of other pathogenic spotted fever group rickettsiae, although the r. japonica isolates cause a persistent ... | 1992 | 1581190 |
| species-specific monoclonal antibodies to rickettsia japonica, a newly identified spotted fever group rickettsia. | a total of 192 hybridomas were developed from mice immunized with rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathogenic species of spotted fever group rickettsiae, rickettsia akari, rickettsia australis, rickettsia conorii, rickettsia rickettsii, and rickettsia sibrica. seven of the species-speci ... | 1990 | 1696280 |
| serologic survey of spotted fever group rickettsiosis on hainan island of china. | a serosurvey for antibodies to rickettsia japonica was conducted on hainan island of china. serum specimens were collected from 1,030 outpatients at hospitals in different parts of the island regardless of their diagnosis. only two among 538 serum specimens collected in baoting and tongshi counties, located in the southern part of the island, were demonstrated to contain antibodies reactive with r. japonica at a high dilution. the specimens also reacted with r. rickettsii at the same titer as wi ... | 1991 | 1808466 |
| [a case report of spotted fever group rickettsiosis first encountered in shimane prefecture, japan]. | rickettsial infections of a spotted fever group have recently been experience in japan. although there have been 50 cases reported by this time, they were exclusively distributed in the areas along the pacific coast. we report in this paper the first case of spotted fever group rickettsiosis in shimane prefecture, an area faced to the japan sea. a 57 year-old man with high fever, general fatigue and rash was admitted to the hospital of shimane medical university at the end of september 1987, who ... | 1990 | 2335750 |
| prevalence of spotted fever group rickettsia antibody in apodemus speciosus captured in an endemic focus in miyazaki prefecture, japan. | fourteen of apodemus speciosus (large japanese field mouse) were captured near the place where one of the patients with spotted fever group rickettsiosis had been infected, in takaoka town, miyazaki prefecture. in the town, four human cases were reported. all of the mice had antibodies against rickettsia japonica and r. montana. the incidence of the antibody was significantly higher in apodemus mice in the area than in those from nonendemic area. | 1990 | 2395224 |
| comparison of antigenic reactivity between intracellular and extracellular rickettsiae of spotted fever group. | antigens of intracellular rickettsiae in vero cells persistently infected with japanese yh strain of the spotted fever group (rickettsia japonica sp. nov.) reacted in the immunofluorescence test with sera from patients to the same extent by igg antibody titration as did antigens of extracellular rickettsiae prepared from culture fluid of lytically infected bhk cells. on the other hand, igm antibody titers measured with intracellular rickettsiae in vero cells were significantly higher in some cas ... | 1989 | 2516664 |
| demonstration of heat-labile and heat-stable epitopes of rickettsia japonica on ultrathin sections. | spotted fever group rickettsiae including rickettsia japonica have two major polypeptides and lipopolysaccharide (lps)-like antigen on the surface. the major part of the antibodies to these polypeptides generated by immunization with live rickettsiae are reactive with heat-labile and conformation-dependent epitopes. | 1994 | 7523765 |
| [serosurvey for spotted fever group rickettsial infection in vertebrates in shimane prefecture]. | we carried out a survey for the prevalence of antibodies to the spotted fever group (sfg) rickettsia in vertebrates such as dogs, cattle, deer, and mice in shimane prefecture. rickettsia japonica was employed as antigen in indirect immunofluorescence (if) tests. the experiment for natural infections using decoy animals was performed in the field of the endemic area. 1. among 115 street dogs, 18.3% possessed the antibodies against sfg rickettsia, while all of the 8 hunting dogs had the antibodies ... | 1995 | 7602185 |
| specific amplification of rickettsia japonica dna from clinical specimens by pcr. | the gene encoding the 17,000-molecular-weight genus-common antigen (17k genus-common antigen) has been cloned and sequenced from rickettsia japonica. the primer pair used for pcr was designed from this sequence. a 357-bp fragment was observed by amplifying the genomic dna from r. japonica and also the dna from blood clots of patients with spotted fever group rickettsiosis. the results indicated that this method is suitable for the diagnosis of spotted fever group rickettsiosis in japan. | 1995 | 7714214 |
| detection of rickettsia japonica in haemaphysalis longicornis ticks by restriction fragment length polymorphism of pcr product. | pcr was applied to the detection of rickettsia japonica, the causative agent of oriental spotted fever (osf), in ticks collected at two sites of the muroto area on shikoku island, a major area in japan where osf is endemic. primer pair rr190.70p and rr190.602n of the r. rickettsii 190-kda antigen gene sequence of regnery and others (r.l. regnery, c.l. spruill, and b.d. plikaytis, j. bacteriol. 173:1576-1589, 1991) primed the dna extracted from haemaphysalis longicornis ticks but not those extrac ... | 1995 | 7790445 |
| nucleotide sequence of polymerase chain reaction product amplified from rickettsia japonica dna using rickettsia rickettsii 190-kilodalton surface antigen gene primers. | the pcr product amplified from rickettsia japonica with the primer pair rr 190.70p and rr 190.602n of r. rickettsii 190-kda antigen gene was cloned into m13mp19 rf dna at the eco ri site and sequenced by chemiluminescent dna sequencing. the sequence revealed a molecular size of 533 base pairs (bp). the primer-flanking region of 491 bp, an open reading frame, was compared with the corresponding region of r. rickettsii, demonstrating 35 nucleotide substitutions in r. japonica. the sequence of prim ... | 1994 | 7898385 |
| differentiation of rickettsia japonica by restriction endonuclease fragment length polymorphism using products of polymerase chain reaction amplification with rickettsia rickettsii 190-kilodalton surface antigen gene primers. | restriction fragment length polymorphism of polymerase chain reaction (pcr) amplification products differentiated rickettsia japonica, a causative agent of oriental spotted fever, from other spotted fever group (sfg) rickettsiae. primer pair rr190. 70p and rr190. 602n of r. rickettsii 190-kda antigen gene sequence primed genomic dnas obtained from r. japonica, type strain yh and strains nt, nk, yki, and tkn. the products were cleaved by psti but not by afai restriction endonuclease. the psti dig ... | 1993 | 7901739 |
| analysis of major surface polypeptides of rickettsia japonica. | major surface polypeptides of rickettsia japonica migrated to the position of 120, 135, and 145 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, when the organisms were solubilized at room temperature. two major bands at the position of 135 and 185 kda were seen, when the organisms were solubilized by heating before electrophoresis. heat-denaturation of the 120- and 145-kda polypeptides in excised gel bands changed their mobility and caused them to migrate to 135- and 185-kda po ... | 1994 | 7968692 |
| rickettsia japonica, the etiologic agent of oriental spotted fever. | | 1993 | 8502180 |
| [spotted fever group rickettsiosis and vectors in kanagawa prefecture]. | primer pairs for pcr were designed from the gene encoding the 17,000-molecular-weight genus-common antigen of rickettsia japonica, rickettsia rickettsii, rickettsia conorii, rickettsia typhi and rickettsia prowazekii. primers r1, r2 were designed for amplifying the genomic dna from spotted fever group (sfg) rickettsiae and epidemic typhus rickettsiae. primers rj5, rj10 were designed for amplifying the genomic dna from only r. japonica. using the primers r1, r2, about a 540-bp fragment was observ ... | 1996 | 8741704 |
| cross-reactivity of rickettsia japonica and rickettsia typhi demonstrated by immunofluorescence and western immunoblotting. | cross-reactivity between rickettsia japonica and r. typhi was observed by immunofluorescence tests using sera from patients with oriental spotted fever (osf), from whom the causative agent was isolated and identified as r. japonica. western immunoblotting with these sera revealed that only the 120-kilodalton surface polypeptide, i.e., rickettsial outer membrane protein (romp) b, has a common antigenicity with the 105-kilodalton surface polypeptide of r. typhi. in some cases, antibodies specifica ... | 1995 | 8789054 |
| epidemiological survey of small rodents for spotted fever rickettsial antibody in hokkaido, japan. | the prevalence of antibody against spotted fever group rickettsia (sfgr) was investigated with rickettsia japonica antigen in small rodents captured in an area in hokkaido, where no human cases of sfgr infection had been reported. antibody against sfgr was found in apodemus speciosus and clethrionomys rufocanus but not in apodemus argenteus. none of these rodents had antibody against r. typhi. this is the first report of the antibody against sfgr in rodents in hokkaido. | 1996 | 8806039 |
| demonstration of a heat-stable 120-kilodalton protein of rickettsia japonica as a spotted fever group-common antigen. | genomic libraries of rickettsia japonica were cloned into an expression vector lambda gt11. a clone expressing a protein reactive with antiserum against 120-kilodalton (kda) proteins, a mixture of heat-modifiable and heat-stable polypeptides, was selected and designated as lambda rj120-1. the expressed protein has a molecular mass of 180 kda. western immunoblotting demonstrated that the expressed protein was a fusion protein with beta-galactosidase. the antiserum against 120-kda proteins was abs ... | 1996 | 8867609 |
| serological survey of spotted fever group rickettsia in wild rats in thailand in the 1970s. | in thailand, the first human cases of spotted fever group rickettsiosis were reported in 1994, but no serosurveys on wild rats have yet been conducted. we investigated the seroepidemiology in wild rats collected in the 1970s from two regions in thailand, and found a 62.2% positive rate of antibodies for spotted fever group rickettsia (sfgr) by the indirect immunofluorescence antibody test. of the antibody-positive rats, 82.2% had higher titers of antibodies against tt-118 than those against rick ... | 1996 | 9013486 |
| citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. | using pcr and an automated laser fluorescent dna sequencer, we amplified and sequenced a 1,234-bp fragment of the citrate synthase-encoding gene (glta) of 28 bacteria belonging to the genus rickettsia. comparative sequence analysis showed that most of the spotted fever group (sfg) rickettsiae belonged to one of two subgroups. the first subgroup included rickettsia massiliae, strain bar 29, rickettsia rhipicephali, "rickettsia aeschlimanni," and rickettsia montana, which have been isolated only f ... | 1997 | 9103608 |
| japanese spotted fever: report of 31 cases and review of the literature. | spotted fever group (sfg) rickettsioses, which are transmitted by ticks, were long thought not to exist in japan. three clinical cases of japanese spotted fever (jsf) were first reported in 1984. the causative agent was isolated and named rickettsia japonica. through october 1996, 31 cases were diagnosed as jsf in tokushima prefecture. infected patients typically had acute high fever, headache, and characteristic exanthema; eschar was observed in 90%. after the discovery of jsf, more than a hund ... | 1997 | 9204291 |
| intracytoplasmic localization of antigenic heat-stable 120- to 130-kilodalton proteins (ps120) common to spotted fever group rickettsiae demonstrated by immunoelectron microscopy. | immunoelectron microscopy demonstrated antigenic heat-stable 120- to 130-kilodalton proteins (ps120) of spotted fever group (sfg) rickettsiae with antiserum against recombinant ps120 of rickettsia japonica. in the case of r. japonica, a major part of the protein was shown to be localized outside the electron-lucent nucleoid-like region in the cytoplasm of the organisms. the other sfg rickettsiae represented a similar localization of the ps120 antigens cross-reactive to that of r. japonica. on th ... | 1997 | 9403508 |
| [incidence of antibodies in raccoon dogs and deer inhabiting suburban areas]. | we evaluated the incidence of antibodies against canine distemper (cd) virus, canine parvovirus, toxoplasma gondii (t. gondii), aujeszky's disease virus, porcine reproductive and respiratory syndrome virus, rickettsia tsutsugamushi, rickettsia japonica, and coxiella burnetii in 30 raccoon dogs and 5 deer captured in suburban areas in kanagawa prefecture. among the raccoon dogs, anti-t. gondii antibody was detected in 1 animal (3.3%) and anti-cd virus antibody in 9 (30.0%). however, antibodies ag ... | 1998 | 9621559 |
| survey of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture, japan. | serological tests were performed to investigate extent of tick-borne diseases in dogs infested with rhipicephalus sanguineus at a kennel in okayama prefecture. three of 22 dogs (13.6%) were positive for ehrlichia canis. two of 19 dogs (10.5%) were positive for rickettsia japonica. three of 22 dogs (13.6%) were positive for babesia gibsoni. none of these animals were positive for coxiella burnetii or hepatozoon canis. a microfilaria was detected in a drop smear of hemolymph from an engorged femal ... | 1998 | 9673953 |
| phylogenetic analysis of spotted fever group rickettsiae by study of the outer surface protein rompa. | rickettsiae are classified in the order rickettsiales and have been included in the alpha subclass of the class proteobacteria on the basis of 16s rrna gene sequence comparison. to estimate the evolutionary forces that have shaped the members of the spotted fever group (sfg) rickettsiae, the ompa gene (apart from the tandem repeat units), encoding an antigenic high-molecular-mass membrane protein specific for the group, was amplified and sequenced from 21 isolates. the phylogenetic relationship ... | 1998 | 9734038 |
| phenotypic and genotypic homogeneity of the strains of rickettsia japonica isolated from patients with oriental spotted fever. | nine pathogenic strains of rickettsia japonica isolated from patients with oriental spotted fever were compared phenotypically and genotypically. constitution and antigenicity of the proteins demonstrated to be the same among strains. polymerase chain reaction (pcr) amplification of the two major outer membrane protein genes (ompa and ompb) and an intracellular spotted fever group-common antigen protein gene (rps120) produced the same sizes of products for all strains. restriction fragment lengt ... | 1999 | 10529114 |
| sequence analysis of the gene encoding a spotted fever group-specific intracytoplasmic protein ps120 of rickettsia japonica. | the 3,438-nucleotide (nt) sequence containing a 3,054-nt open reading frame of the gene (rps120) encoding an antigenic, intracytoplasmic, spotted fever group-specific and heat-stable 120-kilodalton protein (ps120) of rickettsia japonica was determined. the nt and deduced 1,018 amino-acid (aa) sequences were compared to those of r. conorii since only those of this species had been determined among sfg rickettsiae. the homologies of these sequences between r. japonica and r. conorii were considera ... | 1999 | 10585146 |
| sequence analysis of the gene encoding the major outer membrane protein romp b of rickettsia japonica. | the complete nucleotide sequence of the gene encoding romp b (ompb), one of the two major outer membrane antigen proteins, of rickettsia japonica was determined and compared to those of the other spotted fever group and typhus group rickettsiae. open reading frame of the ompb gene of r. japonica consisted of 4,968 nucleotides coding for a putative precursor protein with 1,656 amino acids (aa) in which the n-terminal 1,363 aa and the c-terminal 293 aa encode 135-kilodalton romp b and 32-kilodalto ... | 1999 | 10609616 |
| genetic classification of "rickettsia heilongjiangii" and "rickettsia hulinii," two chinese spotted fever group rickettsiae. | to determine the phylogenetic position of two new rickettsial strains isolated from ticks in china, 16s ribosomal dna, glta, and ompa (apart from the tandem repeat units) genes were amplified by pcr and sequenced. the phylogenetic relationships between these strains and other rickettsiae were inferred from the comparison of sequences of the three genes by the parsimony, neighbor-joining, and maximum-likelihood methods. the results demonstrated that the 054 strain, a rickettsia pathogenic in huma ... | 2000 | 10970415 |
| prevalence of spotted fever rickettsial antibodies in dogs and rodents in the philippines. | antibodies against spotted fever group rickettsiae have been detected in blood samples of dogs and rodents obtained from selected areas in the philippines. in this serosurvey, the positive percentage rates are 8.3% (11/132) in dogs and 12.2% (6/49) in rats. positive results were read from samples tested with rickettsia japonica antigen. no positive result was obtained in blood samples of rats and house mice using r. akari antigen. the findings of this study are the first to confirm the detection ... | 2000 | 11056558 |
| seroprevalence of antibodies against spotted fever group rickettsia among dogs and humans in okinawa, japan. | we evaluated serum antibodies against rickettsia japonica in 517 dogs (430 stray dogs and 87 pet dogs) and 164 humans in okinawa, japan, by indirect immunofluorescence assay. the seropositive rate in stray dogs was significantly higher than that in pet dogs (30.7 versus 4.6%, p<0.01). this high prevalence rate is attributed to the understandably frequent environmental exposure of stray dogs to tick infestation. human samples obtained from okinawa and sapporo also showed a significant difference ... | 2001 | 11270611 |
| fulminant japanese spotted fever associated with hypercytokinemia. | we report a patient with japanese spotted fever caused by rickettsia japonica who developed shock associated with hypercytokinemia. elevated levels of cytokines (macrophage colony-stimulating factor, interleukin 1 beta, interleukin 10, and gamma interferon) decreased rapidly after a combination treatment using an antibiotic (minocycline hydrochloride [mino]) and methylprednisolone; however, tumor necrosis factor alpha levels were increased. the patient's fever relapsed and was resolved only afte ... | 2001 | 11376087 |
| phylogeny of rickettsia spp. inferred by comparing sequences of 'gene d', which encodes an intracytoplasmic protein. | 'gene d' is the ps120-protein-encoding gene, first described in rickettsia conorii and rickettsia japonica. sequence analysis of a 3030 bp fragment of 'gene d' in 24 representatives of the genus rickettsia was carried out to complete phylogenetic analyses previously inferred by comparison of gene sequences encoding citrate synthase, 17 kda antigen and rompa and rompb. the phylogenetic relationships between rickettsiae were inferred from the comparison of both the gene and the derived protein seq ... | 2001 | 11491333 |
| japanese spotted fever associated with multiorgan failure. | a 49-year-old man was admitted to our hospital, with a diagnosis of multiple organ failure, on june 10, 2000. physical examination revealed high fever, generalized maculopapular erythema, and an eschar on his lower leg. laboratory findings revealed severe renal and liver dysfunction, disseminated intravascular coagulation (dic), and markedly elevated soluble interleukin 2-receptor (sil2-r) level (>10 000 u/ml). administration of minocycline was started immediately, with a diagnosis of rickettsia ... | 2001 | 11810592 |
| genetic identification of rickettsiae isolated from ticks in japan. | following the description in japan of japanese spotted fever, caused by rickettsia japonica, a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by pcr and monoclonal antibody testing. we identified these six strains by amplification and sequencing of the genes encoding 16s rrna and citrate synthase. we confirmed that the i ... | 2002 | 12037083 |
| phylogenetic analysis of spotted fever group rickettsiae isolated from ticks in japan. | eight spotted fever group (sfg) rickettsiae isolated from ticks in japan were classified by phylogenetic analysis based on the nucleotide sequences of both the citrate synthase-encoding gene (glta) and 190-kda antigen-encoding gene (rompa). in the phylogenetic tree of glta, strains dt-1 and fla-1 isolated from the dermacentor taiwanensis and haemaphysalis frava ticks, respectively, were placed as rickettsia japonica, and strains io-1, io-2, io-25, im-1 and ip-2 from genus ixodes ticks were place ... | 2002 | 12061626 |
| japanese spotted fever involving the central nervous system: two case reports and a literature review. | japanese spotted fever (jsf), first reported in 1984, is a rickettsial disease caused by rickettsia japonica. until now, affliction of the central nervous system has been rarely reported. here we report two cases of jsf associated with a central nervous system disorder such as meningoencephalitis. | 2002 | 12354906 |
| fulminant japanese spotted fever definitively diagnosed by the polymerase chain reaction method. | a 72-year-old man was admitted to the emergency ward in our hospital on july 20, 2001, because of consciousness disturbance, fever, generalized skin eruption, and severe general weakness beginning 7 days previously. physical examination on admission revealed marked systemic cyanosis, erythema, and purpura. laboratory findings indicated disseminated intravascular coagulation (dic) and multiorgan failure (platelet count, 0.9 x 10(4)/micro l; fibrin degradation product, 110 micro g/ml; c-reactive p ... | 2002 | 12373493 |
| detection of antibodies against spotted fever group rickettsia (sfgr), typhus group rickettsia (tgr), and coxiella burnetii in human febrile patients in the philippines. | a total of 157 sera from febrile patients in the philippine general hospital in manila, luzon, and the northern samar provincial hospital, the philippines, were used. serum antibodies against spotted fever group rickettsia (sfgr) and typhus group rickettsia (tgr) were detected by indirect immunofluorescence test. antibody positive rates were 1.3% for sfgr (rickettsia japonica) and 2.5% for tgr (r. typhus), respectively. rickettsial antibodies in humans in the philippines were found for the first ... | 2003 | 12711823 |
| adherence to and invasion of vero cells by recombinant escherichia coli expressing the outer membrane protein rompb of rickettsia japonica. | recombinant escherichia coli expressing the outer membrane protein rompb of rickettsiae on the surface were generated. the dna corresponding to the open reading frame of the ompb gene of a spotted fever group rickettsia, rickettsia japonica, was amplified by polymerase chain reaction. the amplified fragment was inserted between the sal i and the xho i sites of the expression vector pet-22b(+). e. coli bl21(de3) was transformed by the constructed plasmid. the recombinant bacteria expressed a reco ... | 2003 | 12860694 |
| ectoparasites of the pallas squirrel, callosciurus erythraeus, introduced to japan. | the squirrel callosciurus erythraeus (pallas) (rodentia: sciuridae) was intentionally introduced to japan in 1935 and has become established throughout much of the country. although they live mainly in forests, pallas squirrels come into gardens and are frequently fed by people or kept as pets, so their ectoparasites could be of potential medical as well as veterinary importance. during 2001-2003 we conducted the first ectoparasite survey of pallas squirrels in japan. from 105 c. erythraeus capt ... | 2004 | 15009447 |
| first serologic evidence of human spotted fever group rickettsiosis in korea. | to investigate the prevalence of spotted fever group rickettsioses in korea, a serosurvey of japanese spotted fever rickettsiosis in patients with acute febrile illness was conducted with an indirect immunofluorescence assay. overall, 19.88% of the patients were found to have polyvalent antibody against rickettsia japonica. this study is the first documentation of spotted fever group rickettsiosis in korea. | 2004 | 15131221 |
| detection of rickettsial dna in ixodid ticks recovered from dogs and cats in japan. | dna from ticks recovered from 1137 dogs and 133 cats from all over japan were examined for rickettsia infection by citrate synthase gene (glta)-based pcr and partial nucleotide sequencing. a total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested pcr. sequence analysis was successfully performed on 102 samples. dna of rickettsia japonica or closely related rickettsia spp. strains were detected from 38 ticks in 16 prefectures mainly in weste ... | 2005 | 16397379 |
| adaptive evolution and recombination of rickettsia antigens. | the genus rickettsia consists of intracellular bacteria that cause a variety of arthropod vectored human diseases. i have examined the evolutionary processes that are generating variation in antigens that are potential vaccine candidates. the surface proteins rompa and rompb are subject to intense positive natural selection, causing rapid diversification of their amino acid sequences between species. the positively selected amino acids were mapped and cluster together in regions that may indicat ... | 2006 | 16408241 |
| the major outer membrane protein rompb of spotted fever group rickettsiae functions in the rickettsial adherence to and invasion of vero cells. | the role of one of the major outer membrane proteins, rompb, of spotted fever group rickettsiae was examined. antibodies generated against native rompb inhibited plaque formation by rickettsia japonica in vero cells when applied at the time of inoculation of the rickettsiae. however, antibodies to heat-denatured rompb did not. moreover, the soluble recombinant rompb also inhibited plaque formation to some extent. thus it seems that rompb functions at least in the adherence of rickettsiae to host ... | 2006 | 16500128 |
| japanese spotted fever, south korea. | we describe the first case of japanese spotted fever and the first isolate of spotted fever group rickettsia from a patient in south korea. the isolated rickettsia from the patient was identified as rickettsia japonica by analysis of the nucleotide sequences of 16s rrna, glta, ompa, ompb, and sca4 genes. | 2006 | 16836831 |
| severe japanese spotted fever successfully treated with fluoroquinolone. | a 77 years old woman who had a bite with eschar on her left arm, was admitted to emergency ward in our hospital, because of high fever, severe malaise, skin eruption, and consciousness disturbance beginning 5 days previously. she was diagnosed as japanese spotted fever by seropositive of rickettsia japonica (r. japonica) antibody, and successfully treated with fluoroquinolone, after minocycline hydrochloride had been proven ineffective. r. japonica-specific dna was detected by pcr from the tick: ... | 2006 | 17170509 |
| a case of japanese spotted fever complicated with acute myocarditis. | japanese spotted fever (jsf) is caused by rickettsia japonica. although it induces a variety of complications, acute myocarditis has never been reported as a complication of jsf. we treated a jsf patient who developed acute myocarditis. to our knowledge, this is the first case of jsf complicated with acute myocarditis. | 2007 | 17314430 |
| molecular survey of babesia microti, ehrlichia species and candidatus neoehrlichia mikurensis in wild rodents from shimane prefecture, japan. | a significant number of patients are diagnosed with "fevers of unknown origin" (fuo) in shimane prefecture in japan where tick-borne diseases are endemic. we conducted molecular surveys for babesia microti, ehrlichia species, and candidatus neoehrlichia mikurensis in 62 fuo cases and 62 wild rodents from shimane prefecture, japan. pcr using primers specific for the babesia 18s small-subunit rrna (rdna) gene and anaplasmataceae groesl amplified products from 45% (28/62) and 25.8% (16/62) of captu ... | 2007 | 17446675 |
| [pediatric pneumonia, pleural effusion, and pericarditis following cat scratch disease and serological cross-reactions among bartonella henselae and rickettsia japonica determined by indirect fluorescence antibodies]. | cat scratch disease is associated with a variety of systemic manifestations. we report a pediatric case associated with pneumonia, pleural effusion, and pericarditis. a 3-year-old boy developed prolonged fever unresponsive to antibiotic treatment, including azithromycin and minocycline. although the fever resolved with corticosteroid treatment, bartonella henselae igg titer was positive in indirect fluorescence antibodies, as was rickettsia japonica igg titer. both titers were significantly redu ... | 2007 | 17447481 |
| serological survey of rickettsia japonica infection in dogs and cats in japan. | antibodies against rickettsia japonica in 20 of 1,207 dogs and 5 of 584 cats in japan were detected using immunofluorescence. some antibody-positive animals were detected in niigata and kagawa prefectures, areas in which japanese spotted fever in human patients has never been identified. some animals were positive for antibodies against other new rickettsia species. | 2007 | 17913859 |
| [fulminant japanese spotted fever--the second fatal case in japan]. | a 77-year-old woman who have no past history, was admitted in a local hospital in muroto city, kochi, japan, after several days of fever and severe general fatigue and generalized skin erythema. she was suspected to have japanease spotted fever, which was a local pandemic disease. she was treated with minocycline immediately. the next day, she had consciousness disturbance and low blood pressure. laboratory findings indicated disseminated intravascular coagulation (dic) and multiple organ failur ... | 2008 | 18411764 |
| detection of spotted fever and typhus group rickettsial infection in wild raccoon dogs (nyctereutes procyonoides koreensis) in chonbuk province, korea. | both the spotted fever and the typhus group rickettsioses are potential emerging arthropod-borne infectious disease complexes in korea. wild raccoon dogs (nyctereutes procyonoides koreensis) sheltered at the korean society for the protection of wild animals, chonbuk branch, korea, were sampled for the presence of spotted fever and typhus group rickettsial pathogens. thirty-six serum samples of wild raccoon dogs were examined by an indirect fluorescent antibody test using type-specific yh strain ... | 2008 | 18634203 |
| spotted fever group rickettsia sp. closely related to rickettsia japonica, thailand. | | 2009 | 19331747 |
| identification of spotted fever group rickettsia species by polymerase chain reaction-restriction fragment length polymorphism analysis of the sca4 gene. | dna samples from four rickettsial species in japan--r. japonica aoki, r. asiatica io-1, r. helvetica ip-1, and rickettsia tamurae at-1--were analyzed by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) in which a portion of the sca4 gene was amplified and restriction digested with the mspi enzyme. each spotted fever group rickettsial species exhibited unique restriction profiles after mspi digestion. to examine the possibility of applying pcr-rflp analysis to field s ... | 2009 | 19485769 |
| prediction of rickettsial skin eschars in humans using an experimental guinea pig model. | until now, when a new rickettsia species was isolated in a tick, it was not possible to predict whether it was a human pathogen or if it would cause a skin eschar at the infection site. guinea pigs are injected intradermally with 25 different rickettsia species or subspecies: 16 induced an eschar, 5 induced inflammatory lesions and 4 have no effect. we observed that the occurrence of skin eschars in this model was significantly correlated (p <0.05) with observations of skin eschars in humans (14 ... | 2009 | 19527779 |
| diagnostic assay for rickettsia japonica. | we developed a specific and rapid detection system for rickettsia japonica and r. heilongjiangensis, the causative agents of spotted fever, using a taqman minor groove binder probe for a particular open reading frame (orf) identified by the r. japonica genome project. the target orf was present only in r. japonica-related strains. | 2009 | 19961684 |
| dissemination of spotted fever rickettsia agents in europe by migrating birds. | migratory birds are known to play a role as long-distance vectors for many microorganisms. to investigate whether this is true of rickettsial agents as well, we characterized tick infestation and gathered ticks from 13,260 migratory passerine birds in sweden. a total of 1127 ixodes spp. ticks were removed from these birds and the extracted dna from 957 of them was available for analyses. the dna was assayed for detection of rickettsia spp. using real-time pcr, followed by dna sequencing for spec ... | 2010 | 20052286 |
| nine cases of japan spotted fever diagnosed at our hospital in 2008. | the diagnosis of japan spotted fever (jsf) is very difficult in some cases. the initial diagnosis of jsf is very important to treat. | 2010 | 20465700 |
| detection of spotted fever group rickettsia spp. from bird ticks in the u.k. | migratory birds are known to play a role in the long-distance transportation of microorganisms. to investigate whether this is true for rickettsial agents, we undertook a study to characterize tick infestation in populations of the migratory passerine bird riparia riparia (passeriformes: hirundinidae), the sand martin. a total of 194 birds were sampled and ticks removed from infested birds. the ticks were identified as female ixodes lividus (acari: ixodidae) using standard morphological and mole ... | 2010 | 20546129 |
| serological survey of five zoonoses, scrub typhus, japanese spotted fever, tularemia, lyme disease, and q fever, in feral raccoons (procyon lotor) in japan. | we investigated the seroprevalence of five tick- or mite-borne zoonoses, scrub typhus (orientia tsutsugamushi), japanese spotted fever (rickettsia japonica), tularemia (francisella tularensis), lyme disease (borrelia afzelii and borrelia garinii), and q fever (coxiella burnetii), in feral raccoons (procyon lotor) captured in hokkaido and kanagawa prefectures in japan. of the 559 raccoons captured in hokkaido, 8 (1.4%), 3 (0.5%), 1 (0.2%), and 1 (0.2%) carried antibodies against o. tsutsugamushi ... | 2011 | 20553108 |
| detection of babesia sp. eu1 and members of spotted fever group rickettsiae in ticks collected from migratory birds at curonian spit, north-western russia. | to reveal the prevalence of spotted fever group (sfg) rickettsiae and babesia sp. in ixodes ricinus (l.) ticks from migratory birds, 236 specimens represented 8 species of passeriformes and were collected at curonian spit in kaliningrad enclave of north-western russia. the ticks (total 126) being detached from four bird species, turdus philomelos, fringilla coelebs, parus major, and sturnus vulgaris, were investigated by pcr using the primers rp cs.877p/rp cs.1258n for the detection of rickettsi ... | 2011 | 20553110 |
| evaluation of rickettsia japonica pathogenesis and reservoir potential in dogs by experimental inoculation and epidemiologic survey. | rickettsia japonica pathogenesis and reservoir potential in dogs were evaluated by both experimental inoculation and epidemiologic survey. in the experimental inoculation study, dogs 1 and 2 were pretreated with an immunosuppressive dose of cyclosporine 14 days before inoculation and became ill after exposure to r. japonica. dogs exhibited clinical signs, including fever, anorexia, depression, and decreased water consumption, between 36 and 96 h after inoculation, but these signs disappeared spo ... | 2010 | 20980481 |
| high incidence of rickettsiosis correlated to prevalence of rickettsia japonica among haemaphysalis longicornis tick. | endemic spotted fever group rickettsiosis was reported in shimane prefecture, japan. from an analysis of 14 clinical cases found in the endemic area, the infectious agent of spotted fever group rickettsiosis was identified as rickettsia japonica. in this study, we also found that rickettsia japonica was highly infected with the vector tick, haemaphysalis longicornis, in the endemic area. these findings suggest that the high incidence of rickettsiosis in shimane prefecture can be explained by the ... | 2010 | 21139348 |
| histopathological diagnosis of japanese spotted fever using formalin-fixed, paraffin-embedded skin biopsy specimens: usefulness of immunohistochemistry and real-time pcr analysis. | clin microbiol infect abstract: japanese spotted fever (jsf) is caused by rickettsia japonica, and lethal cases are reported yearly in southwest japan. we thus established the method of diagnosing jsf by immunohistochemistry (ihc) and real-time pcr (rt-pcr) using formalin-fixed, paraffin-embedded skin biopsy specimens. two monoclonal antibodies were used for ihc, and the 17k genus common antigen gene served as the target of rt-pcr. we collected skin biopsy (nôçâ=ôçâ61) and autopsy (nôçâ=ôçâ1) sp ... | 2011 | 21668575 |
| the rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin. | pathogenic rickettsiae species cause high morbidity and mortality, especially r. prowazekii the causative agent of typhus. like many intracellular pathogens, rickettsiae exploit the cytoskeleton to enter and spread within the host cell. here we report that the cell surface antigen sca4 of rickettsiae colocalizes with vinculin in cells at sites of focal adhesions, and that sca4 binds to and activates vinculin through two vinculin binding sites (vbss) that are conserved across all rickettsia. rema ... | 2011 | 21841197 |
| restriction of the growth of a nonpathogenic spotted fever group rickettsia. | the growth kinetics of pathogenic and nonpathogenic rickettsiae were compared to elucidate the mechanism responsible for the pathogenicity of rickettsiae. vero and hela cells derived from mammals were inoculated with a nonpathogenic species of spotted fever group rickettsia, rickettsia montanensis, before being infected with the pathogenic species rickettsia japonica. the mammalian cells became persistently infected with r. montanensis and produced low levels of rickettsiae. on the other hand, s ... | 2011 | 22066520 |
| [clinical investigation of nine pediatric japanese spotted fever cases]. | to clarify the clinical manifestations of pediatric japanese spotted fever (jsf), which remain unclear, we retrospectively reviewed the records of 9 consecutive hospitalized children 5 boys and 4 girls aged 0-15 years (median: 2.3) whose diagnosis was patients with jsf who were serologically confirmed from april 2008 to october 2009. we initially studied the polymerase chain reaction (pcr) assay validity for specific rickettsia japonica dna in the blood. we also studied febrile duration, the his ... | 2011 | 22250454 |
| complete genomic dna sequence of the east asian spotted fever disease agent rickettsia japonica. | rickettsia japonica is an obligate intracellular alphaproteobacteria that causes tick-borne japanese spotted fever, which has spread throughout east asia. we determined the complete genomic dna sequence of r. japonica type strain yh (vr-1363), which consists of 1,283,087 base pairs (bp) and 971 protein-coding genes. comparison of the genomic dna sequence of r. japonica with other rickettsiae in the public databases showed that 2 regions (4,323 and 216 bp) were conserved in a very narrow range of ... | 2013 | 24039725 |
| detection of spotted fever group rickettsiae in ticks from zhejiang province, china. | tick species distribution and prevalence of spotted fever group rickettsiae (sfgr) in ticks were investigated in zhejiang province, china in 2010 and 2011. pcr was used to detect sfgr and positive amplicons were sequenced, compared to published sequences and phylogenic analysis was performed using mega 4.0. a total of 292 adult ticks of ten species were captured and 7.5 % (22/292) of the ticks were pcr-positive for sfg rickettsia. the pcr-positive rates were 5.5 % (6/110) for haemaphysalis longi ... | 2015 | 25633265 |
| genomic analysis of rickettsia japonica strain yht. | rickettsia japonica strain yh, isolated in 1984 in japan, is the type strain of r. japonica, the tick-borne agent of japanese spotted fever. here, we report the 1.33-mb genome of this rickettsial species. | 0 | 23209240 |
| rickettsia vini n. sp. (rickettsiaceae) infecting the tick ixodes arboricola (acari: ixodidae). | recently, a new rickettsia named 'candidatus rickettsia vini' belonging to the spotted fever group has been molecularly detected in ixodes arboricola ticks in spain, the czech republic, slovakia and turkey, with prevalence reaching up to 100 %. the aim of this study was to isolate this rickettsia in pure culture, and to describe it as a new rickettsia species. | 2016 | 27565956 |
| co-circulation of multiple species of rickettsiales bacteria in one single species of hard ticks in shenyang, china. | bacteria in the order rickettsiales include some of the most important zoonotic (re)emerging pathogens for animals and humans. in 2012, a total of 1267 adult haemaphysalis longicornis ticks were collected from domestic animals (cattle and sheep) in shenyang of liaoning province, china. these ticks were grouped into 181 pools (each pool with 6-7 ticks). rickettsiales agents were identified in 93 (51.38%) tick pools using pcr targeting rrs (16s rrna). in addition to 16s rrna gene sequences, glta a ... | 2014 | 25131152 |
| a molecular and serological survey of rickettsiales bacteria in wild sika deer (cervus nippon nippon) in shizuoka prefecture, japan: high prevalence of anaplasma species. | we surveyed rickettsiales bacteria, including rickettsia, ehrlichia, anaplasma, and neoehrlichia, in wild sika deer (cervus nippon nippon) from shizuoka prefecture, japan. in spleen samples from 187 deer, anaplasma phagocytophilum (deer type), a. bovis, and a. centrale were successfully detected by pcr assay targeting to 16s rdna or p44/msp2, and their positive rates were 96.3% (180/187), 53.5% (100/187), and 78.1% (146/187), respectively. additionally, 2 or 3 anaplasma species could be detected ... | 2015 | 25971318 |
| detection of rickettsia spp. and host and habitat associations of fleas (siphonaptera) in eastern taiwan. | rickettsia typhi and rickettsia felis (rickettsiales: rickettsiaceae) are two rickettsiae principally transmitted by fleas, but the detection of either pathogen has rarely been attempted in taiwan. of 2048 small mammals trapped in eastern taiwan, apodemus agrarius pallas (24.5%) and mus caroli bonhote (24.4%) (both: rodentia: muridae) were the most abundant, and m. caroli hosted the highest proportion of fleas (63.9% of 330 fleas). two flea species were identified: stivalius aporus jordan and ro ... | 2012 | 22390200 |
| molecular characterization of 'candidatus rickettsia vini' in ixodes arboricola from the czech republic and slovakia. | the aim of this study was to analyze the prevalence of rickettsiae in the tree-hole tick ixodes arboricola in the czech republic and slovakia. during may to september of 2009 and 2013, bird boxes belonging to three different areas were screened for ticks. in total, 454 nestlings and 109 nests of 10 hole-breeding bird species were examined. ticks were found on ficedula albicollis, parus major, cyanistes caeruleus and sitta europaea and/or in their nests. in total, 166 ticks (17 nymphs, 10 males a ... | 2015 | 25769386 |
| extremely low genomic diversity of rickettsia japonica distributed in japan. | rickettsiae are obligate intracellular bacteria that have small genomes as a result of reductive evolution. many rickettsia species of the spotted fever group (sfg) cause tick-borne diseases known as "spotted fevers". the life cycle of sfg rickettsiae is closely associated with that of the tick, which is generally thought to act as a bacterial vector and reservoir that maintains the bacterium through transstadial and transovarial transmission. each sfg member is thought to have adapted to a spec ... | 2017 | 28057731 |
| case with cellulitis, lymphangitis and subcutaneous nodule suspected due to rickettsia japonica carrying tick bite. | | 2016 | 27786374 |
| [intravenous minocycline and levofloxacin for treatment of two cases of japanese spotted fever]. | we report herein on two cases of japanese spotted fever (jsf) treated with intravenous minocycline (mino) and levofloxacin (lvfx). an 80 year-old woman (case1) and a 63 year-old man (case2) with high fever (> 39 degrees c) and wide-spread skin erythema were admitted because they were suspected of having developed jsf. after admission, we treated them with intravenous mino and lvfx. the patients' fevers were resolved within 36 hours after antibiotics. they were diagnosed as having jsf based on th ... | 2015 | 26630793 |
| hemophagocytic lymphohistiocytosis due to rickettsia japonica in a 3-month-old infant. | | 2015 | 26241724 |
| improvement in early diagnosis of japanese spotted fever by using a novel rick pcr system. | rickettsia diseases, including japanese spotted fever (jsf), are serious infections. delayed diagnosis occasionally results in life-threatening liver disorders and disseminated intravascular coagulation (dic). because of the shortness of the latent period, serological diagnosis is not preferable for early diagnosis of jsf. until now, a polymerase chain reaction (pcr)-based diagnosis method has been used for early diagnosis, and the sensitivity reaches as high as 90% using skin biopsy samples as ... | 2015 | 26130479 |
| [first case report of fatal japanease spotted fever in kumamoto prefecture and the investigation of severe cases in kamiamakusa city]. | an 81 year-old woman whose chief complaint was fever and general fatigue was admitted to our hospital in kamiamakusa city, kumamoto prefecture in october 2013. she was suspected to have japanease spotted fever, which is a local pandemic disease in kamiamakusa, because wide-spreaded skin erythema appeared on the palms of her hands and soles of her feet several days after admission. though there was a delay of more than 6 days after onset, we treated her with minocycline (mino) and levofloxacin (l ... | 2014 | 25672141 |
| a case of japanese spotted fever complicated with central nervous system involvement and multiple organ failure. | japanese spotted fever (jsf), first reported in 1984, is a rickettsial disease characterized by high fever, rash, and eschar formation. a 61-year-old man was admitted to a local hospital in nagasaki city, japan, after several days of high fever and generalized skin erythema. his condition deteriorated and laboratory findings indicated disseminated intravascular coagulation (dic). the patient was transferred to our hospital with mental disturbance and status epilepticus. treatment included minocy ... | 2012 | 22466839 |