| 'candidatus phytoplasma luffae', a novel taxon associated with witches' broom disease of loofah, luffa aegyptica mill. | the phytoplasma associated with witches' broom disease of loofah [luffa aegyptica mill., syn. luffa cylindrica (l.) m.j. roem.] in taiwan was classified in group 16srviii, subgroup a (16srviii-a), based on results from actual and in silico rflp analysis of 16s rrna gene sequences. nucleotide sequencing of pcr-amplified, cloned dna segments revealed rrn interoperon sequence heterogeneity in the loofah witches' broom (lfwb) phytoplasma. whereas the 16s-23s rrna spacer region of rrna contained a co ... | 2017 | 28758630 |
| phylogenetic analysis of "candidatus phytoplasma australiense" reveals distinct populations in new zealand. | abstract the phytoplasma "candidatus phytoplasma australiense" has been reported from new zealand and australia, where it has been associated with a range of host plants, especially since the 1970s. partial tuf gene sequences of 36 new zealand (nz) isolates from four different host genera revealed nine different variants, which clustered into two distinct groups without any obvious correlation with host or geographic region. phylogenetic analysis of these sequences, together with those available ... | 2006 | 18943748 |
| isolation and characterization of the atp-binding cassette (abc) transporter system genes from loofah witches' broom phytoplasma. | a clone containing a 3903 bp ecori-restriction fragment was obtained from a lambda(zap) genomic library of loofah witches' broom (lfwb) phytoplasma by plaque hybridization using a pcr fragment as a probe. sequence analysis revealed that this fragment contained three open reading frames (orfs). the deduced amino acid sequences of orf 1 and orf 2 showed a high homology with the atp-binding proteins of the abc transporter system genes of prokaryotes and eukaryotes, and encoded proteins with a molec ... | 2007 | 17654010 |
| first complete nucleotide sequence and heterologous gene organization of the two rrna operons in the phytoplasma genome. | phytoplasmas are cell-wallless gram-positive low g + c bacteria belonging to the mollicutes that inhabit the cytoplasm of plants and insects. although phytoplasmas possess two ribosomal rna (rrn) operons, only one has been fully sequenced. here, we determined the complete nucleotide sequence of both rrn operons (designated rrna and rrnb) of onion yellows (oy) phytoplasma. both operons have rrna genes organized as 5'-16s-23s-5s-3' with very highly conserved sequences; the 16s, 23s, and 5s rrna ge ... | 2003 | 12804119 |
| organization of ribosomal rna genes from a loofah witches' broom phytoplasma. | using the technique of integrative mapping with three vectors carrying chromosomal rdna sequences, one of two rrna operons of loofah witches' broom (lfwb) phytoplasma was constructed. this is the first complete rrna operon of a phytoplasma to be reported. the operon has a context of 5'-16s-23s-5s-3' with a trna(ile) gene in the its and trna(val) and trna(asn) genes downstream from the 5s rrna gene. although the other operon has not been cloned, the dna sequence of a pcr-amplified product shows t ... | 2001 | 11244569 |