| genetic diversity among phytoplasmas infecting opuntia species: virtual rflp analysis identifies new subgroups in the peanut witches'-broom phytoplasma group. | phytoplasmas were detected in cactus (opuntia species) plants exhibiting witches'-broom disease symptoms in yunnan province, south-western china. comparative and phylogenetic analyses of 16s rrna gene sequences indicated that an overwhelming majority of the cactus-infecting phytoplasmas under study belonged to the peanut witches'-broom phytoplasma group (16srii). genotyping through use of computer-simulated restriction fragment length polymorphism (rflp) analysis of 16s rrna genes revealed a rem ... | 2008 | 18523193 |
| multilocus sequences confirm the close genetic relationship of four phytoplasmas of peanut witches'-broom group 16srii-a. | four witches'-broom diseases associated with arachis hypogaea (peanut), crotalaria pallida, tephrosia purpurea, and cleome viscosa were observed in hainan province, china during field surveys in 2004, 2005, and 2007. in previously reported studies, we identified these four phytoplasmas as members of subgroup 16srii-a, and discovered that their 16s rrna gene sequences were 99.9-100% identical to one another. in this study, we performed extensive phylogenetic analyses to elucidate relationships am ... | 2014 | 23686459 |
| high-throughput transcriptome analysis of the leafy flower transition of catharanthus roseus induced by peanut witches'-broom phytoplasma infection. | peanut witches'-broom (pnwb) phytoplasma are obligate bacteria that cause leafy flower symptoms in catharanthus roseus. the pnwb-mediated leafy flower transitions were studied to understand the mechanisms underlying the pathogen-host interaction; however, our understanding is limited because of the lack of information on the c. roseus genome. in this study, the whole-transcriptome profiles from healthy flowers (hfs) and stage 4 (s4) pnwb-infected leafy flowers of c. roseus were investigated usin ... | 2014 | 24492256 |
| identification of putative effector genes and their transcripts in three strains related to 'candidatus phytoplasma aurantifolia'. | molecular mechanisms underlying phytoplasma interactions with host plants are largely unknown. in this study attempts were made to identify effectors of three phytoplasma strains related to 'ca. p. aurantifolia', crotalaria phyllody (crp), faba bean phyllody (fbp), and witches' broom disease of lime (wbdl), using information from draft genome of peanut witches' broom phytoplasma. seven putative effectors were identified in wbdl genome (sap11, sap21, eff64, eff115, eff197, eff211 and effsap67), f ... | 2017 | 28454710 |
| identification of a novel subgroup 16srii-u phytoplasma associated with papaya little leaf disease. | papaya is an important fruit crop cultivated in tropical and subtropical regions. papaya little leaf (pll) disease was observed in china. the phytoplasma 16s rrna gene was detected from symptomatic papaya trees via pcr using phytoplasma universal primers p1/p7 followed by r16f2n/r16r2. no amplification products were obtained from templates of asymptomatic papaya trees. these results indicated a direct association between phytoplasma infection and pll disease. comparative and phylogenetic analyse ... | 2016 | 27266888 |
| comparative analysis of the peanut witches'-broom phytoplasma genome reveals horizontal transfer of potential mobile units and effectors. | phytoplasmas are a group of bacteria that are associated with hundreds of plant diseases. due to their economical importance and the difficulties involved in the experimental study of these obligate pathogens, genome sequencing and comparative analysis have been utilized as powerful tools to understand phytoplasma biology. to date four complete phytoplasma genome sequences have been published. however, these four strains represent limited phylogenetic diversity. in this study, we report the shot ... | 2013 | 23626855 |