| molecular phylogeny of the genus frankia and related genera and emendation of the family frankiaceae. | the members of the actinomycete genus frankia are nitrogen-fixing symbionts of may species of woody dicotyledonous plants belonging to eight families. several strains isolated from diverse actinorhizal plants growing in different geographical areas were used in this study. the phylogenetic relationships of these organisms and uncharacterized microsymbionts that are recalcitrant to isolation in pure culture were determined by comparing complete 16s ribosomal dna sequences. the resulting phylogene ... | 1996 | 8573482 |
| morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces. | in an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16s rdna sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. all except one strain, which was found to be a bacillus, were members of the order actinomycetales. the majority of the strains either were closely related to geodermatophilus obscurus, which was al ... | 1996 | 8661940 |
| polyamine profiles within genera of the class actinobacteria with ll-diaminopimelic acid in the peptidoglycan. | polyamine patterns of coryne- and nocardioform representatives of the class actinobacteria with ll-diaminopimelic acid in the peptidoglycan, comprising strains of the genera aeromicrobium, nocardioides, intrasporangium, terrabacter, terracoccus, propioniferax, friedmanniella, microlunatus, luteococcus and sporichthya, were analysed. the different polyamine patterns were in good agreement with the phylogenetic heterogeneity within this group of actinomycetes. strains of the closely related genera ... | 1999 | 10028260 |
| selective isolation and distribution of sporichthya strains in soil | a simplified enrichment method in which centrifugation is used for selective isolation of sporichthya strains from soil is described. gellan gum plus 2 mm cacl2 stimulated growth of sporichthya polymorpha kcc a0089 so that this organism was readily recognized on an isolation plate. high yields of motile spores were obtained by using a flooding solution containing 0.1% skim milk in 10 mm mops (morpholinepropanesulfonic acid) (ph 8.0) and then incubating the preparation at 27 degrees c for 60 min ... | 1999 | 10223982 |
| sporichthya brevicatena sp. nov. | a strain was isolated from soil that formed short chains of aerial mycelium dividing into motile spores on an agar medium, but not substrate mycelium. the organism had menaquinone 9(h8), ll-diaminopimelic acid in the cell wall amino acids and a dna g + c content of 71 mol%. on the basis of the morphological and chemotaxonomic characteristics and phylogenetic analysis, this strain was classified into the genus sporichthya. dna-dna hybridization revealed that the strain differed from the known spe ... | 1999 | 10555360 |
| application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. | the present paper describes a simple enrichment technique which enables rapid and selective isolation of diverse zoosporic actinomycete genera directly from soil and plant litter. this technique, designated the rehydration and centrifugation (rc) method, consists of immersing the air-dried source material in 10 mm phosphate buffer containing 10% soil extract, letting the preparation stand at 30 degrees c for 90 min, followed by centrifugation of the fluid at 1,500 x g for 20 min. portions of the ... | 2000 | 11204769 |
| quadrisphaera granulorum gen. nov., sp. nov., a gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules. | a gram-positive bacterium, designated strain ag019(t), was isolated by micromanipulation from aerobic granules obtained from a laboratory-scale sequencing batch reactor. this isolate grew axenically as cocci clustered predominantly in tetrads, and was morphologically similar to the dominant organisms observed in the biomass. the morphology also resembled that of the tetrad-forming organisms commonly seen in activated sludge samples. strain ag019(t) was found to be an oxidase-negative, catalase-p ... | 2005 | 16166665 |
| catenulispora acidiphila gen. nov., sp. nov., a novel, mycelium-forming actinomycete, and proposal of catenulisporaceae fam. nov. | a novel, gram-positive bacterial strain was isolated from forest soil. among species with validly published names, the 16s rrna gene sequence is related most closely (approx. 93 % similarity) to that of sporichthya polymorpha dsm 43042(t). however, differently from this species, it forms both vegetative and aerial mycelia. the aerial hyphae are straight to slightly flexuous, starting to septate to form chains of more than 20 cylindrical spores with a rugose surface. the strain is acidophilic, wi ... | 2006 | 16902001 |
| culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp. | a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ... | 2008 | 18670903 |
| phylogenetic analysis of actinobacterial populations associated with antarctic dry valley mineral soils. | despite the apparent severity of the environmental conditions in the mcmurdo dry valleys, eastern antarctica, recent phylogenetic studies conducted on mineral soil samples have revealed the presence of a wide diversity of microorganisms, with actinobacteria representing one of the largest phylotypic groups. previous metagenomic studies have shown that the majority of antarctic actinobacterial populations are classified as 'uncultured'. in this study, we assessed the diversity of actinobacteria i ... | 2009 | 19278445 |