| purification and characterization of a xylanase from the thermophilic ascomycete thelavia terrestris 255b. | thielavia terrestris 255b, a thermophilic ascomycete, produced two major forms of xylanase with pis of 4.6 (xylanase i) and 6.1 (xylanase ii). the latter enzyme could be purified to greater than 99% homogeneity using anion-exchange chromatography and gel filtration. xylanase ii had a mol wt of 25.7 kda (sds-page) and a ph and a temperature optimum of 3.6-4.0 and 60-65 degrees c, respectively. the ratio of the enzyme's activity against xylan and carboxymethylcellulose was 500-1000 to 1, indicatin ... | 1992 | 1622204 |
| a comparison of two xylanases from the thermophilic fungi thielavia terrestris and thermoascus crustaceus. | two thermophilic xylanases (xylanase ii from thielavia terrestris 255b and the 32-kda xylanase from thermoascus crustaceus 235e) were studied to determine if they had different and complementary modes of action when they hydrolysed various types of xylans. partial amino acid sequencing showed that these two enzymes belonged to different families of beta-1,4-glycanases. xylanase ii achieved faster solubilization of insoluble xylan whereas the 32-kda xylanase was more effective in producing xylose ... | 1993 | 7764332 |
| cloning, heterologous expression, and characterization of thielavia terrestris glucoamylase. | thielavia terrestris is a soil-borne thermophilic fungus whose molecular/ cellular biology is poorly understood. only a few genes have been cloned from the thielavia genus. we detected an extracellular glucoamylase in culture filtrates of t. terrestris and cloned the corresponding glaa gene. the coding region contains five introns. based on the amino acid sequence, the glucoamylase was 65% identical to neurospora crassa glucoamylase. sequence comparisons suggested that the enzyme belongs to the ... | 2003 | 14665735 |
| production of microbial biomass protein from potato processing wastes by cephalosporium eichhorniae. | the use of cephalosporium eichhorniae 152 (atcc 38255) (reclassified as acremonium alabamense; see addendum in proof), a thermophilic, acidophilic, amylolytic fungus, for the conversion of potato processing wastes into microbial protein for use as animal feed was studied. the fungus was not inhibited by alpha-solanine or beta-2-chaconine, antimicrobial compounds in potatoes, or by morpholine or cyclohexylamine (additives to steam used in the peeling process) at levels likely to be encountered in ... | 1987 | 16347277 |
| ecology of thermophilic fungi in mushroom compost, with emphasis on scytalidium thermophilum and growth stimulation of agaricus bisporus mycelium. | twenty-two species of thermophilic fungi were isolated from mushroom compost. scytalidium thermophilum was present in the compost ingredients, fresh straw, horse droppings, and drainage from compost and dominated the fungal biota of compost after preparation. of 34 species of thermophilic fungi tested, 9 promoted mycelial growth of agaricus bisporus on sterilized compost: chaetomium thermophilum, an unidentified chaetomium sp., malbranchea sulfurea, myriococcum thermophilum, s. thermophilum, sti ... | 1994 | 16349175 |
| relationship of the morphogenesis of thielavia terrestris (apinis) malloch et cain with previous culture conditions. | | 2006 | 16572809 |
| efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose. | this study examined the cellulytic effects on steam-pretreated barley straw of cellulose-degrading enzyme systems from the five thermophilic fungi chaetomium thermophilum, thielavia terrestris, thermoascus aurantiacus, corynascus thermophilus, and myceliophthora thermophila and from the mesophile penicillum funiculosum. the catalytic glucose release was compared after treatments with each of the crude enzyme systems when added to a benchmark blend of a commercial cellulase product, celluclast, d ... | 2006 | 16599567 |
| oxidoreductive cellulose depolymerization by the enzymes cellobiose dehydrogenase and glycoside hydrolase 61. | several members of the glycoside hydrolase 61 (gh61) family of proteins have recently been shown to dramatically increase the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases. however, purified gh61 proteins have neither demonstrable direct hydrolase activity on various polysaccharide or lignacious components of biomass, nor an apparent hydrolase active site. cellobiose dehydrogenase (cdh) is a secreted flavocytochrome produced by many cellulose degrading fungi with no wel ... | 2011 | 21821740 |
| comparative genomic analysis of the thermophilic biomass-degrading fungi myceliophthora thermophila and thielavia terrestris. | thermostable enzymes and thermophilic cell factories may afford economic advantages in the production of many chemicals and biomass-based fuels. here we describe and compare the genomes of two thermophilic fungi, myceliophthora thermophila and thielavia terrestris. to our knowledge, these genomes are the first described for thermophilic eukaryotes and the first complete telomere-to-telomere genomes for filamentous fungi. genome analyses and experimental data suggest that both thermophiles are ca ... | 2011 | 21964414 |
| a low molecular mass cutinase of thielavia terrestris efficiently hydrolyzes poly(esters). | a low molecular mass cutinase (designated ttcuta) from thielavia terrestris was purified and biochemically characterized. the thermophilic fungus t. terrestris cau709 secreted a highly active cutinase (90.4 u ml(-1)) in fermentation broth containing wheat bran as the carbon source. the cutinase was purified 19-fold with a recovery yield of 4.8 %. the molecular mass of the purified ttcuta was determined as 25.3 and 22.8 kda using sds-page and gel filtration, respectively. ttcuta displayed optimal ... | 2012 | 23271406 |