| [distinct esterase profiles of levinea maloatica and of levinea amalonatica]. | | 1975 | 811411 |
| distinctive electrophoretic patterns of esterases from levinea malonatica, levinea amalonatica and citrobacter. | esterases of 42 strains of levinea malonatica, levinea amalonatica and citrobacter were analysed by horizontal slab electrophoresis in polyacrylamide-agarosegel using several synthetic substrates. on the basis of esterase zymograms a distinctive pattern was established for each of the three species. levinea malonatica was characterized by two major bands: one hydrolysing acetate esters but not butyrate esters; and the other hydrolysing alpha-naphthyl acetate and reacting weakly with alpha-naphth ... | 1977 | 856943 |
| serotyping of strains belonging to the citrobacter-levinea group isolated from diagnostic material. | a proposal is presented for the serotyping of citrobacter-levinea strains: 9 serotypes for citrobacter diversus (levinea malonatica) and 13 serotypes for h2s-negative, indole-positive variants of citrobacter freundii (levinea amalonatica), which showed a considerable antigenic diversity. by means of both kinds of antiserum types 80 per cent of the strains (l. malonatica 100 per cent, l. amalonatica 72 per cent) from a series of 106 strains isolated from different diagnostic material were serolo ... | 1976 | 936828 |
| [antigenic relationships between "levinea amalonatica" and "shigella dysenteriae" and "boydii" (author's transl)]. | twenty-eight strains among thirty-five levinea amalonatica show o antigenic relationships with shigella as follows: with s. dysenteriae 3 (5 strains), s. dysenteriae 4 (1 strain), s. dysenteriae 5 (3 strains), s. dysenteriae 7 (11 strains), s. boydii 6 (7 strains), and with both s. boydii 15 and s. dysenteriae 8 (1 strain). these strains do not induce purulent keratogenous conjunctivitis in guinea pigs as shigella do. | 1975 | 1098541 |
| mobilization of nonconjugative pbr322-derivative plasmids from laboratory strains of escherichia coli to bacteria isolated from seawater. | mobilization of derivatives of plasmids pbr322 and pbr325 was shown to occur between escherichia coli k12 strains in lb-broth at 37 degrees c, provided a mobilizer plasmid (f') was present either together with the nonconjugative plasmid or in a second donor strain. evidence from restriction endonuclease analysis suggested that the mobilization was facilitated by a transposition phenomenon involving the "gamma-delta" sequence of f'. it was shown that mobilization of a derivative of pbr325 from e. ... | 1992 | 1341984 |
| cloning and nucleotide sequence of the gene (cita) encoding a citrate carrier from salmonella typhimurium. | a cryptic citrate transport gene (cita) from salmonella typhimurium chromosome was cloned and its nucleotide sequence was determined. the cloned plasmid conferred citrate-utilizing ability on wild-type escherichia coli, which cannot grow on citrate as the sole source of carbon. the resultant e. coli transformant was able to transport citrate. a 1,302-base-pair open reading frame with a preceding ribosomal binding site was found in the cloned dna fragment. the 434-amino-acid protein that could be ... | 1991 | 1718953 |
| [biochemical properties of avian citrobacter amalonaticus strains]. | a report is given on the biochemical properties of c. amalonaticus-strains of avian origin. some strains show decisive deviations of the "norm", usually established from mammalian strains and details are given in a table. the results are discussed and the strains considered to be avian specific. | 1991 | 1786041 |
| chromosomally-mediated beta-lactamases produced by levinea amalonatica with activity against methoxyimino-cephalosporins. | levinea amalonatica strain a2370 was isolated from the blood culture of a patient hospitalized in charles nicolle hospital (tunis) during july 1986 and had decreased susceptibility to cefotaxime. isoelectric focusing of crude extracts from this strain demonstrated three bands of beta-lactamase activity which focused at ph 5.4, 5.5 and 6.05. the three bands were separated and analysed for their kinetic properties. one enzyme (pi 5.4) had the properties of a tem-1 beta-lactamase and did not confer ... | 1991 | 2055810 |
| nucleotide sequence of a citrate utilization gene from citrobacter amalonaticus. | the chromosomal dna fragment (bamhi-bglii fragment of 2,972 base pairs) conferring citrate utilization in citrobacter amalonaticus atcc 25405 was cloned and sequenced. two genes (cita and citb) identified in the cit+ determinant were found in a bamhi-bglii fragment from c. amalonaticus. southern dna-dna hybridization experiments and the construction of cit- mutants of c. amalonaticus showed that c. amalonaticus has a single copy of the cit gene on the chromosome. | 1989 | 2644212 |
| imipenem and cilastatin in acute osteomyelitis and suppurative arthritis. therapy in infants and children. | twenty-five infants and children with acute osteomyelitis (n = 7), suppurative arthritis (n = 11), or both (n = 7) were treated with imipenem and cilastatin sodium. patients ranged in age from 5 months to 11.3 years. needle aspiration of infected sites was performed in all patients, and 11 (44%) required further surgical drainage. imipenem and cilastatin sodium in a dosage of 100 mg/kg/d was used for children 3 years of age or younger, while older ones received 60 mg/kg/d intravenously, divided ... | 1987 | 3544811 |
| biochemical identification of new species and biogroups of enterobacteriaceae isolated from clinical specimens. | in 1972 there were only 11 genera and 26 species in the family enterobacteriaceae. today there are 22 genera, 69 species, and 29 biogroups or enteric groups. this paper is a review of all of the new organisms. it has a series of differential charts to assist in identification and a large chart with the reactions of 98 different organisms for 47 tests often used in identification. a simplified version of this chart gives the most common species and tests most often used for identification. the so ... | 1985 | 3881471 |
| conservation of dna sequences for plasmid-mediated citrate utilization within the enterobacteria. | southern blot dna-dna hybridization experiments with a cloned cit+ dna fragment as a probe showed that the plasmid-mediated cit+ determinants from four cit plasmids (r726, poh3001, poh3035, and poh30221) were all homologous. sequences homologous to the plasmid-borne cit+ gene were also found in total bacterial dna isolated from salmonella paratyphi b, salmonella enteritidis, salmonella typhimurium lt-2, citrobacter freundii, atcc 8090, citrobacter amalonaticus atcc 25405, klebsiella pneumoniae i ... | 1986 | 3941048 |
| the serology of citrobacter koseri, levinea malonatica, and levinea amalonatica. | | 1974 | 4599660 |
| citrobacter infections in humans: experience at the seattle veterans administration medical center and a review of the literature. | the genus citrobacter is a distinct group of human pathogens comprising three species: citrobacter freundii (biotypes a and b), citrobacter amalonaticus, and citrobacter diversus. in this review the clinical and microbiologic experience during 1972-1978 at the seattle veterans administration medical center (seattle, wash.) with 298 isolates of citrobacter is analyzed in relation to a survey of the literature. the most common sources of citrobacter isolates were urine, sputum, and soft tissue exu ... | 1980 | 6763304 |
| [utilization and fermentation of 2-ketogluconate by "enterobacteriaceae" (author's transl)]. | two methods were compared: 1) the ability to use 2-ketogluconate (2-kg) as source of carbon and energy in defined medium, and 2) the ability to produce acid by fermentation of 2-kg (0.5 g/100 ml) in peptone water with phenol red. results of growth on, and acid production from, 2-kg were identical. a total of 990 strains was studied. no strain of escherichia coli, shigella (4 species), salmonella (4 sub-genera), yersinia pestis, y. pseudotuberculosis, edwardsiella (2 species), proteus vulgaris, p ... | 1981 | 7235456 |
| evaluation of rapid one system for identification of 379 strains in the family enterobacteriaceae and oxidase-negative, gram-negative nonfermenters. | the ability of the rapid one system (innovative diagnostic systems, inc., norcross, ga.) to identify 364 strains in the family enterobacteriaceae and 15 oxidase-negative, gram-negative, nonfermentative rods was evaluated. kits were inoculated with no. 2 mcfarland standard suspensions, and reactions were interpreted after 4 h of incubation at 35 degrees c. overall, the method correctly identified (to the species level or to the genus level for salmonellas and non-shigella sonnei shigella species) ... | 1994 | 8027345 |
| classification of citrobacteria by dna hybridization: designation of citrobacter farmeri sp. nov., citrobacter youngae sp. nov., citrobacter braakii sp. nov., citrobacter werkmanii sp. nov., citrobacter sedlakii sp. nov., and three unnamed citrobacter genomospecies. | dna relatedness studies (hydroxyapatite method) were done on 112 strains of citrobacteria. by using the recommended definition of a genomospecies 11 genomospecies were identified in the genus citrobacter. these genomospecies were separable by their biochemical profiles. citrobacter koseri (citrobacter diversus) and citrobacter amalonaticus proved to be homogeneous species, as previously described. c. amalonaticus biogroup 1, as described by farmer et al. (j. clin. microbiol. 21:46-76, 1985), was ... | 1993 | 8240948 |
| purification and characterization of three carboxylesterases from enterobacteriaceae. | the carboxylesterases from proteus vulgaris, salmonella enterica and citrobacter amalonaticus were purified 104-, 95- and 120-fold, respectively by chromatography. the enzymes had similar catalytic activities but differed considerably in their inactivation by heat, di-isopropyl fluorophosphate and cd2+, zn2+, hg2+ and cu2+. quantitative neutralization of hydrolytic activity with specific immunoglobulins indicated that the three enzymes were antigenically distinct. | 1993 | 8472928 |
| mutational analysis of the cita citrate transporter from salmonella typhimurium: altered substrate specificity. | the cita citrate transporter in salmonella typhimurium is encoded by the cita gene and consists of 434 amino acid residues that probably include 12 membrane-spanning segments [shimamoto. t., et al. (1991) j. biochem. 110, 22-28]. cita mutants with altered substrate specificities were isolated by in vitro mutagenesis using nitrous acid. the mutants could grow on isocitrate as a sole carbon source which normally cannot be transported well by the cita transporter of s. typhimurium. the mutation sit ... | 1996 | 8806660 |
| cloning, nucleotide sequencing, and expression of the 3-methylaspartate ammonia-lyase gene from citrobacter amalonaticus strain yg-1002. | the gene coding for 3-methylaspartate ammonia-lyase (3-methylaspartase, mal, ec 4.3.1.2) from citrobacter amalonaticus strain yg-1002 (tpu 6323) was cloned onto plasmid pbluescript ii ks(+), and the nucleotide sequence of the 1239-bp open reading frame (orf), consisting of 413 codons, was identified as the mal gene coding for mal. the predicted polypeptide has 62.5% identity with mal from the obligate anaerobe, clostridium tetanomorphum ncimb 11547. orf1, which showed 58.6% and 58.8% identities ... | 1998 | 9830098 |
| cloning and expression in escherichia coli of an azoreductase gene from clostridium perfringens and comparison with azoreductase genes from other bacteria. | a genomic library of clostridium perfringens atcc 3626 was constructed in phage lambda gt11 and screened with an antibody against the c. perfringens azoreductase, which catalyzes the reduction of azo dyes to aromatic amines. a positive recombinant phage, containing a 3.8 kb dna fragment insert was selected and purified. lytic and lysogenic escherichia coli cultures infected with the recombinant phage had higher azoreductase activity than cultures infected only with the vector lambda gt11. the 3. ... | 1999 | 10071864 |
| a novel ctx-m beta-lactamase (ctx-m-8) in cefotaxime-resistant enterobacteriaceae isolated in brazil. | to estimate the diversity of extended-spectrum beta-lactamases in brazil, 18 strains from different species of the family enterobacteriaceae exhibiting a positive double-disk synergy test were collected by a clinical laboratory from several hospitals in rio de janeiro, brazil, in 1996 and 1997. four strains (proteus mirabilis, enterobacter cloacae, enterobacter aerogenes, and citrobacter amalonaticus) hybridized with a 550-bp ctx-m probe. the p. mirabilis strain produced a ctx-m-2 enzyme. the e. ... | 2000 | 10858358 |
| outbreak of nosocomial infections due to extended-spectrum beta-lactamase-producing strains of enteric group 137, a new member of the family enterobacteriaceae closely related to citrobacter farmeri and citrobacter amalonaticus. | a member of the enterobacteriaceae initially identified as kluyvera cryocrescens by the microscan gram-negative combo 13 panel caused an outbreak of nosocomial infections in four patients (pneumonia, n = 2; urinary tract infection, n = 1; wound infection, n = 1) and urinary tract colonization in one patient. when the strains were tested by the enteric reference laboratory of the centers for disease control and prevention, biochemical results were most compatible with yersinia intermedia, kluyver ... | 2000 | 11060050 |
| identification and functional characterization of arylamine n-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid. | arylamine n-acetyltransferase activity has been described in various bacterial species. bacterial n-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. we characterized these enzymes further by steady-state kinetics, time-dependent inhibition, and dna hybridization in 40 species, mostly from the human intestinal microflora. we report for the first time n-acetyltransferase activity in 11 ... | 2001 | 11344150 |
| further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens. | rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ... | 2001 | 11491080 |
| intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae. | intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ... | 2001 | 11544246 |
| crystallization and preliminary x-ray analysis of citrobacter amalonaticus methylaspartate ammonia lyase. | methylaspartate ammonia lyase (mal) catalyses the reversible alpha,beta-elimination of ammonia from l-threo-(2s,3s)-3-methylaspartic acid to give mesaconic acid. crystals of citrobacter amalonaticus mal have been obtained by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. three crystal forms were obtained from identical crystallization conditions, two of which (forms a and b) diffract to high resolution, whilst the third form diffracted poorly. crystals of ... | 2001 | 11717518 |
| insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase. | methylaspartate ammonia lyase (mal) catalyzes the magnesium-dependent reversible alpha,beta-elimination of ammonia from l-threo-(2s,3s)-3-methylaspartic acid to mesaconic acid. the 1.3 a mad crystal structure of the dimeric citrobacter amalonaticus mal shows that each subunit comprises two domains, one of which adopts the classical tim barrel fold, with the active site at the c-terminal end of the barrel. despite very low sequence similarity, the structure of mal is closely related to those of r ... | 2002 | 11796115 |
| low-virulence citrobacter species encode resistance to multiple antimicrobials. | citrobacter spp. are gram-negative commensal bacteria that infrequently cause serious nosocomial infections in compromised hosts. they are often resistant to cephalosporins due to overexpression of their chromosomal beta-lactamase. during a recent study of multidrug-resistant enterobacteriaceae (mdre) in solid-organ transplant patients, we found that almost half of patients colonized with mdre carried one or more cefpodoxime-resistant citrobacter freundii, citrobacter braakii, or citrobacter ama ... | 2002 | 12384364 |
| chromosome-encoded ambler class a beta-lactamase of kluyvera georgiana, a probable progenitor of a subgroup of ctx-m extended-spectrum beta-lactamases. | a chromosome-encoded beta-lactamase gene, cloned and expressed in escherichia coli from kluyvera georgiana reference strain cuetm 4246-74 (dsm 9408), encoded the extended-spectrum beta-lactamase klug-1, which shared 99% amino acid identity with the plasmid-mediated beta-lactamase ctx-m-8. this work provides further evidence that kluyvera spp. may be the progenitor(s) of ctx-m-type beta-lactamases. | 2002 | 12435721 |
| role of citrobacter amalonaticus and citrobacter farmeri in dissimilatory perchlorate reduction. | the article deals with the novel physiological function of dissimilatory perchlorate reduction by strains jb101 and jb109 isolated from a laboratory-enriched mixed consortium originating from a sewage treatment facility. the biochemical and physiological data of the strains showed good correspondence with members of the family enterobacteriaceae. the partial 16s rdna sequence of the strains jb101 and jb109 had similarity of 99.8% to citrobacter amalonaticus and 98% to citrobacter farmeri, respec ... | 2004 | 15069667 |
| citrobacter koseri and citrobacter amalonaticus isolates carry highly divergent beta-lactamase genes despite having high levels of biochemical similarity and 16s rrna sequence homology. | isolates previously identified as citrobacter diversus are now known as citrobacter koseri. we measured sequence variation at the beta-lactamase structural gene among a group of clinical isolates originally identified as c. diversus by api 20e profiling. | 2004 | 15128725 |
| degradation of cyanophycin by sedimentibacter hongkongensis strain ki and citrobacter amalonaticus strain g isolated from an anaerobic bacterial consortium. | using a combination of various enrichment techniques, the strictly anaerobic, gram-positive, endospore-forming bacterium sedimentibacter hongkongensis strain ki as revealed by 16s rrna analysis and the gram-negative enterobacterium citrobacter amalonaticus strain g as revealed by physiological tests were isolated from an anaerobic cyanophycin (cgp)-degrading bacterial consortium. s. hongkongensis strain ki is the first anaerobic bacterium with the ability to hydrolyze cgp to beta-asp-arg and bet ... | 2005 | 16000772 |
| dissemination of ctx-m-type extended-spectrum beta-lactamase genes to unusual hosts. | a citrobacter amalonaticus and a morganella morganii producing the ctx-m-1 extended-spectrum beta-lactamase (esbl) were isolated from an area where this enzyme is now widespread in escherichia coli. this is the first report of ctx-m-1 in the former species. in both cases the esbl determinant was possibly acquired by these unusual hosts in vivo, after coinfection with e. coli strains carrying conjugative plasmids encoding ctx-m-1. | 2005 | 16081971 |
| enteric fever-like illness caused by infection with citrobacter amalonaticus. | 'enteric fever' is a potentially fatal, severe systemic disease, which is encountered worldwide. traditionally, enteric fever refers to a bacteremic illness caused by members of certain salmonella serotypes, notably: salmonella typhi, a gram-negative bacterium, and to a lesser extent, salmonella paratyphi a, b and c. in addition, other non-salmonella organisms may produce a syndrome clinically indistinguishable from "enteric fever". brucella sp., campylobacter sp., edwardsiella tarda, enterobact ... | 2005 | 16083226 |
| development of a rapid immunochromatographic strip for detection of escherichia coli o157. | we developed an immunochromatographic (ic) strip for the rapid detection of escherichia coli o157 in enriched samples. murine monoclonal antibody to e. coli o157:h7 lipopolysaccharide was conjugated with 40 nm of colloidal gold particles by the citrate method. the specificity of the ic strip was determined using 48 pure-cultured bacteria, including 32 e. coli strains and 16 non-e. coli strains. regardless of h serotype, e. coli o157 strains produced a positive signal, whereas the others, represe ... | 2005 | 16245720 |
| characterization of the chromosomal class a beta-lactamase cko from citrobacter koseri. | the gene bla(cko) encoding the chromosomal class a beta-lactamase of citrobacter koseri was cloned and sequenced. cko was found to display only 41% identity with sed-1 from citrobacter sedlakii and 36% with cdia from citrobacter amalonaticus (formerly citrobacter diversus). no transcriptional regulator was found upstream from bla(cko). silent and missense mutations were detected in four bla(cko) genes amplified from different c. koseri clinical isolates, but the cko variants displayed identical ... | 2006 | 16445758 |
| emergence of arma and rmtb aminoglycoside resistance 16s rrna methylases in belgium. | 16s rrna methylase-mediated high-level resistance to aminoglycosides has been reported recently in clinical isolates of gram-negative bacilli only from a limited number of countries. this study was conducted to investigate the occurrence of this type of resistance in clinical isolates of enterobacteriaceae from two belgian hospitals and the characteristics of the strains. | 2007 | 17224412 |
| real-time pcr method for salmonella spp. targeting the stn gene. | to develop a real-time pcr assay for salmonella spp. targeting the stn gene. | 2007 | 17241358 |
| a novel phytase appa from citrobacter amalonaticus cgmcc 1696: gene cloning and overexpression in pichia pastoris. | a novel phytase gene appa, with upstream and downstream sequences from citrobacter amalonaticus cgmcc 1696, was cloned by degenerate polymerase chain reaction (pcr), and thermal asymmetric interlaced (tail) pcr and was overexpressed in pichia pastoris. sequence analysis revealed one open reading frame that consisted of 1311 bp encoding a 436-amino-acid protein, which had a deduced molecular mass of 46.3 kda. the phytase appa belongs to the histidine acid phosphatase family and exhibits the highe ... | 2007 | 17657539 |
| carbon and energy balances of glucose fermentation with hydrogenproducing bacterium citrobacter amalonaticus y19. | for the newly isolated h2-producing chemoheterotrophic bacterium citrobacter amalonaticus y19, anaerobic glucose metabolism was studied in batch cultivation at varying initial glucose concentrations (3.5- 9.5 g/l). the carbon-mass and energy balances were determined and utilized to analyze the carbon metabolic-pathways network. the analyses revealed (a) variable production of major metabolites (h2, ethanol, acetate, lactate, co2, and cell mass) depending on initial glucose levels; (b) influence ... | 2008 | 18388473 |
| metallo-β-lactamases among enterobacteriaceae from routine samples in an italian tertiary-care hospital and long-term care facilities during 2008. | the emergence of metallo-β-lactamase (mbl)-producing enterobacteriaceae is a serious public health concern. producers have been repeatedly isolated from patients and long-term care facility (ltcf) residents around bolzano, and we sought to assess their prevalence and clinical impact. all routine enterobacteriaceae isolates from a bolzano tertiary-care hospital and associated long-term care facilities in 2008 (n = 5500) were screened for mbls, with case details reviewed for the source patients. i ... | 2011 | 20345467 |
| chemical composition and antibacterial activity of essential oils of tripleurospermum disciforme in three developmental stages. | tripleurospermum disciforme (c.a. mey) schultz bip. (asteraceae) is a widespread biennial species which also has traditional medicinal uses. according to the few recent studies, essential oils of this species exhibit anti-inflammatory, antispasmodic, antiseptic, antifungal, antiulcer, and antioxidant activity. objective: the chemical compositions of the hydrodistilled oils of t. disciforme of iranian origin are studied in the stages of prior to flowering, flowering, and post flowering, for the f ... | 2010 | 20795784 |
| quorum sensing activity of citrobacter amalonaticus l8a, a bacterium isolated from dental plaque. | cell-cell communication is also known as quorum sensing (qs) that happens in the bacterial cells with the aim to regulate their genes expression in response to increased cell density. in this study, a bacterium (l8a) isolated from dental plaque biofilm was identified as citrobacter amalonaticus by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry (ms). its n-acylhomoserine-lactone (ahl) production was screened by using two types of ahl biosensors namely chr ... | 2016 | 26860259 |
| prevalence of extended spectrum beta-lactamase production among uropathogens in south mumbai and its antibiogram pattern. | β-lactams are the most widely used group of antimicrobials. however, increasing resistance to these valuable drugs in uropathogens, mediated principally by β-lactamases, has become a major concern. the present study was undertaken to determine the prevalence of extended spectrum β-lactamase (esbl) producers in clinical isolates of urine specimens, collected from various healthcare centres across south mumbai. a total of 195 gram negative urine isolates were identified as pseudomonas aeruginosa ( ... | 2012 | 27418912 |
| molecular characteristics of extended-spectrum cephalosporin-resistant enterobacteriaceae from humans in the community. | to investigate the molecular characteristics of extended-spectrum cephalosporin (esc)-resistant enterobacteriaceae collected during a cross-sectional study examining the prevalence and risk factors for faecal carriage of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in humans living in areas with high or low broiler density. | 2015 | 26029910 |
| microbial degradation of a recalcitrant pesticide: chlordecone. | chlordecone (kepone®) is a synthetic organochlorine insecticide (c10cl10o) used worldwide mostly during the 1970 and 1980s. its intensive application in the french west indies to control the banana black weevil cosmopolites sordidus led to a massive environmental pollution. persistence of chlordecone in soils and water for numerous decades even centuries causes global public health and socio-economic concerns. in order to investigate the biodegradability of chlordecone, microbial enrichment cult ... | 2016 | 28066351 |
| purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties. | the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ... | 2017 | 28110033 |
| citrobacter amalonaticus y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery. | citrobacter amalonaticus y19 is a good biocatalyst for production of hydrogen (h2) from oxidation of carbon monoxide (co) via the so-called water-gas-shift reaction (wgsr). it has a high h2-production activity (23.83 mmol h2 g(-1) cell h(-1)) from co, and can grow well to a high density on various sugars. however, its h2-production activity is expressed only when co is present as an inducer and in the absence of glucose. | 2017 | 28360938 |
| microbial degradation of paclitaxel using citrobacter amalonaticus rashtia isolated from pharmaceutical wastewater: kinetic and thermodynamic study. | paclitaxel is a highly toxic anticancer agent which is used in a wide range against ovarian, breast, lung, and prostate cancers. paclitaxel is manufactured recently in the north of iran which may lead to the introduction of the drug into the environment via pharmaceutical wastewater. to our knowledge, paclitaxel degradation is currently performed using physicochemical methods and biological degradation of paclitaxel has not been reported. in this study, a paclitaxel degrading bacterium was isola ... | 2016 | 27339310 |
| enhanced biohydrogen production from corn stover by the combination of clostridium cellulolyticum and hydrogen fermentation bacteria. | hydrogen was produced from steam-exploded corn stover by using a combination of the cellulolytic bacterium clostridium cellulolyticum and non-cellulolytic hydrogen-producing bacteria. the highest hydrogen yield of the co-culture system with c. cellulolyticum and citrobacter amalonaticus reached 51.9 l h2/kg total solid (ts). the metabolites from the co-culture system were significantly different from those of the mono-culture systems. formate, which inhibits the growth of c. cellulolyticum, coul ... | 2016 | 27150511 |
| citrobacter amalonaticus human urinary tract infections, marseille, france. | citrobacter amalonaticus is a bacterium that has rarely been reported as a human pathogen. here we report four cases of c. amalonaticus infections occurring in patients hospitalized in marseille, france, and review all cases described in the published literature. | 2016 | 26958347 |
| detoxification of mercury pollutant leached from spent fluorescent lamps using bacterial strains. | the spent fluorescent lamps (sfls) are being classified as a hazardous waste due to having mercury as one of its main components. mercury is considered the second most toxic heavy metal (arsenic is the first) with harmful effects on animal nervous system as it causes different neurological disorders. in this research, the mercury from phosphor powder was leached, then bioremediated using bacterial strains isolated from qatari environment. leaching of mercury was carried out with nitric and hydro ... | 2016 | 26725036 |
| combined strategies for improving expression of citrobacter amalonaticus phytase in pichia pastoris. | phytase is used as an animal feed additive that degrades phytic acid and reduces feeding costs and pollution caused by fecal excretion of phosphorus. some phytases have been expressed in pichia pastoris, among which the phytase from citrobacter amalonaticus cgmcc 1696 had high specific activity (3548 u/mg). improvement of the phytase expression level will contribute to facilitate its industrial applications. | 2015 | 26410558 |
| complete genome sequence of novel carbon monoxide oxidizing bacteria citrobacter amalonaticus y19, assembled de novo. | we report here the complete genome sequence of citrobacter amalonaticus y19 isolated from an anaerobic digester. pacbio single-molecule real-time (smrt) sequencing was employed, resulting in a single scaffold of 5.58mb. the sequence of a mega plasmid of 291kb size is also presented. | 2015 | 26210290 |
| co-addition of manure increases the dissipation rates of tylosin a and the numbers of resistance genes in laboratory incubation experiments. | the behavior of veterinary antibiotics in the soil is commonly studied using the following methods to add antibiotics to the soil: (a) adding manure collected from animals fed a diet that includes antibiotics; (b) adding antibiotic-free animal manure spiked with antibiotics; and (c) the direct addition of antibiotics. however, most studies have only used methods (b) and (c) in their research, and few studies have simultaneously compared the different antibiotic addition methods. this study used ... | 2015 | 25958362 |
| citrobacter amalonaticus phytase on the cell surface of pichia pastoris exhibits high ph stability as a promising potential feed supplement. | phytase expressed and anchored on the cell surface of pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst. in this study, the phytase gene of citrobacter amalonaticus was fused with the pichia pastoris glycosylphosphatidylinositol (gpi)-anchored glycoprotein homologue gcw61. phytase exposed on the cell surface exhibits a high activity of 6413.5 u/g, with an opt ... | 2014 | 25490768 |
| the effect of heavy metals on microbial community structure of a sulfidogenic consortium in anaerobic semi-continuous stirred tank reactors. | the effect of heavy metals on community structure of a heavy metal tolerant sulfidogenic consortium was evaluated by using a combination of denaturing gradient gel electrophoresis (dgge) of 16s rrna gene and dissimilatory sulfite reductase (dsrb) gene fragments, 16s rrna gene cloning analysis and fluorescence in situ hybridization (fish). for this purpose, four anaerobic semi-continuous stirred tank reactors (referred as r1-r4) were run in parallel for 12 weeks at heavy metal loading rates of 1. ... | 2014 | 23880736 |
| glycerol assimilation and production of 1,3-propanediol by citrobacter amalonaticus y19. | citrobacter amalonaticus y19 (y19) was isolated because of its ability for carbon monoxide-dependent hydrogen production (water-gas shift reaction). this paper reports the assimilation of glycerol and the production of 1,3-propanediol (1,3-pdo) by y19. genome sequencing revealed that y19 contained the genes for the utilization of glycerol and 1,2-propanediol (pdu operon) along with those for the synthesis of coenzyme b12 (cob operon). on the other hand, it did not possess the genes for the ferme ... | 2013 | 23377788 |
| first report of peritoneal dialysis-related peritonitis caused by citrobacter amalonaticus. | | 2015 | 22383728 |
| [enterobacteriaceae and beta-lactams : wild susceptibility patterns]. | four susceptibility patterns of wild types of enterobacteria against old beta-lactams including aminopenicillins, carboxypenicillins and first-generation cephalosporins were individualized during the 1980s : susceptible, penicillinase low level, cephalosporinase and a combination of penicillinase and cephalosporinase. such indirect detection of a mechanism of resistance allowed an interpretative reading for this class of antibiotics. at the present time, seven susceptibility patterns were propos ... | 2012 | 22280847 |