| "white-wild" (variola-like) poxvirus strains from rodents in equatorial africa. | strains of variola-like virus were isolated from rodents, namely 1 each from mastomys coucha (fam. muridae) and helioscorus rufobrachium (fam. sciuridae) in the bumba zone of the equatorial province of the zaire republic. attempts to isolate the virus from 48 monkeys were negative. neutralizing antibodies were found in 1 of 7 monkey (cercopithecus ascanius schmidti) sera examined as well as in the serum of h. rufobrachium from which the virus was isolated. | 1976 | 7947 |
| studies on schistosoma bovis in ethiopia. | schistosoma bovis occurs in at least seven of the 14 rovinces of ethiopia. results of faecal and snail surveys in three foci are reported. adwa. one collection showed that nine out of 26 bulinids were infected with s. bovis. the snail host was a tetraploid form of bulinus (n = 36). the examination of 200 specimens of cattle faces revealed no s. bovis eggs, which was attributed to poor technique or light infection. gewani. the snail host was bulinus abyssinicus, which was also infected with s. ha ... | 1975 | 808181 |
| litomosoides carinii: extraction of the microfilarial sheath components and antigenicity of the sheath fractions. | microfilarial sheaths of litomosoides carinii were isolated and extracted with 2% sodium dodecyl sulfate (sds) and 5% 2-mercaptoethanol (2me). extraction with sds alone did not alter the ultrastructure of the sheaths and yielded five polypeptides (27-67 kda) that were not recognized by antibodies of infected hosts but reacted with antibodies to host-serum proteins. 2me treatment caused partial solubilization of the sheaths (45% as determined by amino acid analysis), which could be further improv ... | 1992 | 1438136 |
| an electrophoretic polymorphism in salivary amylases (amy-1) of mastomys (praomys coucha). | an electrophoretic polymorphism of salivary amylases (amy-1) in mastomys (praomys coucha) (mwc, mrj and mcc strains) was detected. amylase in mwc or mrj saliva, which migrated fast toward the anode, was designated as amy-1a, and that in mcc saliva migrating slowly as amy-1b. salivary amylases are controlled by a pair of codominant alleles at a single autosomal locus (amy-1). no polymorphism was seen in pancreatic amylases (amy-2). the frequencies of these phenotypes did not differ between the se ... | 1990 | 1702847 |
| the infection characteristics of a south african isolate of schistosoma mansoni: a comparison with a puerto rican isolate in balb/c mice and mastomys coucha. | the infection characteristics of a south african (rsa) laboratory isolate of schistosoma mansoni were compared with those of a widely studied puerto rican (pr) isolate in balb/c mice and mastomys coucha over the period of 5-12 weeks after infection. the infectivity, maturation and, in particular, the fecundity of the two schistosomes were found to differ markedly. tissue egg-accumulation rates of the rsa strain were substantially lower than those of the pr strain. although the maturation of both ... | 1991 | 1902955 |
| immune responses of two mastomys sibling species to yersinia pestis. | this study assessed the in vitro cell-mediated immune responses of mastomys natalensis, with a diploid chromosome number of 2n = 32, and mastomys coucha, with a diploid chromosome number of 2n = 36, to yersinia pestis. splenic mononuclear (mn) cells of uninfected m. natalensis proliferated in response to crude fraction 1 of y. pestis and two subfractions derived from fraction 1 in vitro. proliferation was dose dependent and followed the time kinetics of other well-known mitogens. further charact ... | 1991 | 2037358 |
| intestinal parasites of conventionally maintained balb/c mice and mastomys coucha and the effects of a concomitant schistosome infection. | a longitudinal study was carried out to identify the spectrum of intestinal parasites present in conventionally maintained balb/c mice and mastomys coucha and to determine the effects of concomitant schistosome infections on their parasite status. six parasites were observed during the course of the study, namely the nematodes aspiculuris tetraptera and syphacia obvelata, entamoeba muris and the flagellates trichomonas muris, spironucleus muris and chilomastix spp. although the 2 rodents shared ... | 1990 | 2118582 |
| adrenocortical zonation of inbred wild-coloured mastomys, praomys coucha: a new border zone in the cortex of females. | adrenal glands were examined in the inbred strain of wild-coloured mastomys (praomys coucha), mwc. the most characteristic findings were emergence of a border zone between the zona fasciculata and z. reticularis of the cortex in females but not in males, and persistence of broad z. reticularis in males. the border zone was typically comprised of vacuolated cell layers, nodular layers and fibrous tissue. vacuolated cells increased in number during pregnancy and partially disappeared during lactat ... | 1990 | 2304326 |
| mastomys natalensis or mastomys coucha. correct species designation in animal experiments. | haemoglobin electrophoresis and chromosome analysis indicate that the widely used multimammate rat (chamois coloured) as experimental animal belongs to the species mastomys coucha (smith, 1836) and not, as commonly assumed, to mastomys natalensis (smith, 1834). | 1990 | 2382103 |
| comparative tests for detection of plague antigen and antibody in experimentally infected wild rodents. | the enzyme-linked immunosorbent assay (elisa) was compared with other standard tests for detection of plague (yersinia pestis) antibody and antigen in multimammate mice (mastomys coucha and m. natalensis) which were experimentally infected and then killed at daily intervals postinoculation. for detection of antibody in sera from m. natalensis, the immunoglobulin g (igg) elisa was equivalent in sensitivity to passive hemagglutination and more sensitive than the igm elisa and complement fixation. ... | 1986 | 3097065 |
| phenotypic differences in schistosoma mattheei ova from populations sympatric and allopatric to s. haematobium. | schistosoma mattheei ova were collected from cattle in different localities in south africa and after hatching, miracidia were used to infest bulinus (physopsis) globosus. cercariae harvested from these snails were used to infest the definitive host praomys (mastomys) coucha and eggs from the resulting female s. mattheei were collected. these ova were compared with a schistosoma haematobium x s. mattheei hybrid similarly collected from an infested p. (m.) coucha. the results indicate that s. mat ... | 1986 | 3725328 |
| advantages and use of laboratory-reared african multimammate mice (mastomys coucha) in the diagnosis of plague. | | 1982 | 7181544 |
| surface antigens of litomosoides carinii microfilariae: agglutinating antibodies react with sheath components of 40 and 120 kilo dalton molecular mass. | this study was conducted to identify surface antigens of the microfilarial sheath of litomosoides carinii which are accessible to antibodies. rabbit antisera were raised against the soluble and insoluble fractions of purified sheaths by extracting them with a buffer containing 2-mercaptoethanol and sodium dodecylsulphate. these sera and rabbit hyperimmune sera directed against homogenates of total microfilariae, mature (i.e. microfilariae liberating) female parasites and excretory-secretory prod ... | 1994 | 7520154 |
| litomosoides carinii: macrofilariae-derived glycolipids--chromatography, serology and potential in the evaluation of anthelminthic efficacy. | a preliminary characterization of the glycolipids of litomosoides carinii macrofilariae, resolved according to their chromatographic, chemical and serological properties, has been performed. emphasis has been placed on the neutral fraction glycolipids. these are separable on thinlayer chromatography into two groups of fast and slow migrating band components, that differ in their migration, differential chemical staining and serological traits, respectively. serological analyses have been accompl ... | 1994 | 7708429 |
| ivermectin-induced cell-dependent lethal effects on litomosoides carinii microfilariae in vitro. | ivermectin affected the motility of litomosoides carinii microfilariae in vitro in a dose dependent manner but did not completely immobilize the larvae and had no lethal effects when tested up to a concentration of 1000 ng/ml. however, killing of microfilariae was induced by ivermectin in vitro in the presence of spleen cells of mastomys coucha or rats within 14 h. optimum effects occurred at drug levels of 10-100 ng ivermectin/ml. addition of infection serum led to increased cytotoxicity when c ... | 1994 | 7716399 |
| [phylogenetic relationships of mastomys to mouse and rat deduced from satellite dna sequences]. | mastomys is a rodent with a intermediate size between mouse and rat, and classify as a subgenus of praomys coucha. our study determined the phylogenetic evolutional relationships of mastomys to mouse and rat deduced from satellite dna sequences. genomic dna was extracted from each liver and digested by restriction enzymes (ecori and bglii). after separation and purification, we obtained these ecori/bglii-0.5 kbp repeated elements and determined the nucleotide sequences. the similarities and spee ... | 1994 | 7925629 |
| antifilarial activity of macrocyclic lactones: comparative studies with ivermectin, doramectin, milbemycin a4 oxime, and moxidectin in litomosoides carinii, acanthocheilonema viteae, brugia malayi, and b. pahangi infection of mastomys coucha. | the avermectins ivermectin and doramectin and the milbemycins milbemycin a4 oxime and moxidectin were tested for filaricidal activity in mastomys coucha infected with litomosoides carinii, acanthocheilonema viteae, brugia malayi, and b. pahangi. single subcutaneous doses of 0.005-5 mg/kg (l. carinii), 0.0005-0.5 mg/kg (a. viteae), 0.5 and 5 mg/kg (b. malayi), and 5 mg/kg (b. pahangi) were injected. necropsies were performed 42 days after treatment. the avermectins caused a strong and rapid reduc ... | 1994 | 7939168 |
| the infection characteristics of the antelope schistosomes, schistosoma margrebowiei and s. leiperi, in inbred balb/c mice and in mastomys coucha. | detailed studies were carried out to assess the infection characteristics of the antelope schistosomes in balb/c mice and mastomys coucha. both schistosome species reached sexual maturity in these hosts. however, they differed markedly in terms of egg production rates and preferred sites of tissue egg deposition. tissue egg deposition commenced between 4 and 5 weeks in the case of schistosoma margrebowiei and between 6 and 7 weeks in the case of s. leiperi. tissue egg accumulation rates of s. le ... | 1994 | 8006380 |
| experimental chemotherapy of filariasis: comparative evaluation of the efficacy of filaricidal compounds in mastomys coucha infected with litomosoides carinii, acanthocheilonema viteae, brugia malayi and b. pahangi. | eleven types/classes of compound with antifilarial activity were comparatively evaluated in mastomys coucha infected with litomosoides carinii, acanthocheilonema viteae, brugia malayi or b. pahangi. the paper deals with the efficacy of (i) predominantly microfilaricidal compounds [diethylcarbamazine, levamisole, avermectins (ivermectin, milbemycin), nitrofurans (nitrofurantoin, hydroxymethylnitrofurantoin, nifurtimox, furazolidone, furapyrimidone), organophosphorals (metrifonate, haloxon), and a ... | 1993 | 8094587 |
| igg subclasses of the multimammate rat, mastomys coucha: isolation and characterization of igg1 and igg2. | the objective of the study was to characterize the igg-system of mastomys coucha and to provide antibodies specific for igg subclasses of this animal species. serum proteins binding to protein a at ph 8.0 could be eluted in two distinct peaks at ph 7.5 - 6.2 and ph 5.0 - 3.9. the fractions were analysed by sds-page and immunoelectrophoresis. both tests suggested an igg character of the fractions. based on their migration behavior in the electric field they were classified as igg1 (anodic positio ... | 1994 | 8193173 |
| effect of ivermectin on two filaria-vector pairs. brugia malayi-aedes aegypti; litomosoides sigmodontis-bdellonyssus bacoti. | the effect of ivermectin was studied on two filaria-vector pairs, brugia malayi-aedes aegypti and litomosoides sigmodontis-bdellonyssus bacoti. the rodent hosts, respectively mastomys coucha and meriones unguiculatus, were treated with ivermectin doses of 0.05 mg/kg, or 0.2 mg/kg or 2 mg/kg. batches of vectors were fed on rodents, infected or not, treated or not, from h7 to d43 post-ivermectin. vector survival was observed and dissections were performed to study the filarial development. it appe ... | 1993 | 8215116 |
| evaluation of resistant-reversal, cdri compound 87/209 and its possible mode of action in rodent experimental malaria. | the resurgence of malaria in form of resistance against chloroquine (cq) has decreased the importance of the drug as a chemotherapeutic agent. if an agent in combination with cq can make cq resistant plasmodia susceptible to cq, the problem of drug resistance may then be solved. use of conventional drugs like verapamil, desipramine along with cq suggested the feasibility of this approach. this report is concerned with a new class of compound, cdri compound 87/209 (15 mg/kg b. wt.) which is given ... | 1995 | 8525290 |
| induction and prevention of shock-like lethal side effects after microfilaricidal treatment in filariae infected rodents. | in contrast to human carriers of microfilariae, filariae infected rodents generally tolerate an effective microfilaricidal treatment without obvious signs of adverse reactions. the study shows, however, that also the filariae (litomosoides carinii, brugia malayi) infected rodent mastomys coucha can be rendered sensitive to side effects of the treatment by the administration of d-galactosamine (d-gal), due to reduction of liver utp levels. independent of the drug (diethylcarbamazine, ivermectin, ... | 1995 | 8826101 |
| setaria digitata microfilaraemia in mastomys coucha: an animal model for chemotherapeutic and immunobiological studies. | intraperitoneal implantation of adult gravid females of the bovine filarial parasite, setaria digitata in mastomys coucha was found to induce microfilaraemia lasting for about 125 days. the microfilariae (mf) could be detected as early as 4 days post-implantation (p.i.) and peak levels of about 30 mf in 20 microliters of blood were observed by 21 days. a significant positive correlation was found between mf density and the body weight of recipients pre-implantation. the implanted adult worms wer ... | 1996 | 8873474 |
| mitochondrial heme oxygenase of mastomys coucha. | while studying the fate of heme generated during malaria infection, it was observed that mitochondrial preparations were highly enriched with heme compared to other subcellular particles. with this background, the present study aimed to determine the status of mitochondrial heme oxygenase and compare it with the microsomal enzyme. mitochondrial and microsomal preparations were obtained from liver, spleen, kidney and brain of normal, inducer (cobalt chloride and hemin)-treated and plasmodium berg ... | 1996 | 8930130 |
| acanthocheilonema viteae: vaccination with irradiated l3 induces resistance in three species of rodents (meriones unguiculatus, mastomys coucha, mesocricetus auratus). | three species of rodents were immunized with 50 irradiated (35 krad) stage-3 larvae (l3) of the filaria acanthocheilonema viteae and challenged with an infection of normal l3. the immunization induced a significant reduction of the worm burden developing from the challenge infection in all host species, the jird (meriones unguiculatus), the multimammate rat (mastomys coucha) and the golden hamster (mesocricetus auratus). the induced resistance was highest in jirds (92.5 +/- 9.7) followed by gold ... | 1997 | 9018308 |
| antibodies to diethylcarbamazine potentiate the antifilarial activity of the drug. | the antifilarial drug diethylcarbamazine citrate (dec) is known to mediate in vivo microfilaricidal activity in conjunction with the host immune system. in this study passive transfer of antibodies to dec elicited by immunization with methyl piperazine carboxylic acid (mpca) coupled to bovine serum albumin (bsa), was found to potentiate microfilaricidal activity of subcurative doses of dec in setaria digitata infected mastomys coucha. active immunization of microfilaraemic animals with mpca-bsa ... | 1997 | 9149286 |
| antifilarial activity of a synthetic marine alkaloid, aplysinopsin (cdri compound 92/138). | cdri compound 92/138, a synthetic analogue of aplysinopsin, was evaluated in experimental filarial infections, litomosoides carinii in cotton rats (sigmodon hispidus) and acanthocheilonema viteae in mastomys coucha. the compound killed 63.8 and 90% of adult l. carinii and a. viteae at doses of 30 and 50 mg/kg (i.p.) respectively given for 5 days. by the oral route, at 100 mg/kg for 5 days the compound caused 50.9 and 57% mortality of adult l. carinii and a. viteae, respectively. at 200 mg/kg adm ... | 1997 | 9236820 |
| 1h magnetic resonance imaging and 31p magnetic resonance spectroscopy in experimental filariasis. | 1h magnetic resonance imaging and 31p magnetic resonance spectroscopy (mrs) have been carried out in experimental rodent filariasis, i.e., acanthocheilonema viteae infection in the rodent host, mastomys coucha. the t2-weighted image of the infected host shows fine hyperintense thread like structures of adult filariid nests in the cervical region. 31p mrs of normal and infected hosts, localized over the same region of interest, show seven major peaks corresponding to phosphomonoesters (including ... | 1997 | 9408140 |
| cellular immune response of mastomys and gerbils in experimental filariasis. | to determine mitogenic and antigen-specific cellular immune responses of two species of rodents, viz. meriones unguiculatus and mastomys coucha to assess the usefulness of the a. viteae/mastomys model for cellular immune studies in experimental filariasis. | 1998 | 9537274 |
| the fate and acute toxicity of aflatoxin b1 in the mastomys and rat. | the fate and acute toxicity of aflatoxin b1 (afb1) were studied in the mastomys (praomys coucha) and compared with fischer rats. the experiment regarding the fate of [3h]afb1 showed that the radioactivity was excreted mainly through the feces, more rapidly in the mastomys than in the rat, regardless of whether [3h]afb1 was given orally or intravenously. the levels of radioactivity bound to the liver dna were lower in the mastomys than in the rat, indicating that the levels of afb1 binding to the ... | 1998 | 9604291 |
| brugia malayi in mastomys coucha: establishment in immunosuppressed animals. | investigations on various aspects of human filariasis using target filarial parasite, brugia malayi is jeopardised to a great extent due to its prolonged incubation period and poor harvest from the existing experimental animal models. to obviate these difficulties it was contemplated to establish b. malayi infection in immunosuppressed mastomys coucha. cortisone, a well known immunosuppressant, was used at 10 mg/kg dose level subcutaneously in two courses each of 5 days duration. the first cours ... | 1998 | 9698261 |
| picroliv prevents oxidation in serum lipoprotein lipids of mastomys coucha infected with plasmodium berghei. | picroliv, an iridoid glycoside mixture from the root and rhizome of picrorhiza kurrooa, at the dose of 6 mg/kg p.o. for two weeks provided significant protection against the generation of lipid peroxidation products in serum beta-lipoproteins of p. berghei infected m. coucha. incubation of normal rat hepatocytes with very low density lipoprotein or low density lipoprotein isolated from infected animals caused significant generation of lipid peroxides followed by a decrease in the viability of th ... | 1998 | 9717447 |
| juvenile female litomosoides sigmodontis produce an excretory/secretory antigen (juv-p120) highly modified with dimethylaminoethanol. | a 120 kda antigen produced by juvenile female litomosoides sigmodontis (juv-p120) was isolated and purified. the amino acid composition of the molecule was determined. juv-p120 was shown to be highly modified with n,n-dimethyl-aminoethanol (28.4 mol%). treatment of juv-p120 with potassium hydroxide (beta-elimination) or with sodium m-periodate leads to the destruction of epitopes recognized by antibodies immune affinity-purified with isolated juv-p120. juvenile l. sigmodontis were shown to relea ... | 1998 | 9774792 |
| brugia malayi in mastomys coucha: establishment in immunosuppressed animals. | investigations on various aspects of human filariasis using target filarial parasite, brugia malayi is jeopardised to a great extent due to its prolonged incubation period and poor harvest from the existing experimental animal models. to obviate these difficulties it was decided to establish b. malayi infection in immunosuppressed mastomys coucha. cortisone, a well-known immunosuppressant, was used at 10-mg/kg dose level subcutaneously in two courses each of 5 days duration. the first course was ... | 1998 | 9821467 |
| metal chelators/antioxidants: approaches to protect erythrocytic oxidative stress injury during plasmodium berghei infection in mastomys coucha. | desferal, n-acetyl penicillamine (metal chelators) and propylgallate, catechin, and reduced glutathione (antioxidants) suppressed the erythrocytic oxidative damage generated during plasmodium berghei infection in mastomys coucha. superoxide anion and lipid peroxide levels were increased and on the contrary, superoxide dismutase activity was noticeably decreased in the infected erythrocytes. metal chelators/antioxidant treatment to infected animals resulted in restoration of o(2)(-), lpo and sod ... | 1999 | 10479467 |
| bacillus sphaericus interferes with the development of brugia malayi in aedes aegypti. | aedes aegypti (black-eyed liverpool strain) were exposed to a sublethal dose (ld25) of bacillus sphaericus and were fed to mastomys coucha infected with brugia malayi. the development of the filarial parasite was found to be arrested mostly at the second larval stage. the infection (p < 0.05), infectivity rates (p < 0.001) and l3 load (p < 0.001) were found to be reduced significantly in the treated group. | 1999 | 10526424 |
| metabolite mapping of human filarial parasite, brugia malayi with nuclear magnetic resonance. | metabolite mapping of human filarial parasite, brugia malayi was carried out in vitro as well as in situ in host mastomys coucha by 31p nuclear magnetic resonance (nmr) spectroscopy. detection of parasites by visualizing contrast spots due to pathologic changes was observed by 1h magnetic resonance imaging (mri). major metabolites of adult b. malayi observed by 31p-nmr spectroscopy were of sugar phosphates (sp), phosphomonoesters (pme), glycerophosphoryl-ethanolamine (gpe), -choline (gpc), phosp ... | 1999 | 10609999 |
| antifilarial activity of some 2h-1-benzopyran-2-ones (coumarins). | six synthetic 2h-1-benzopyran-2-one (cournarin) derivatives (cdri compounds # 1, 2, 3, 4, 5 and 6) were evaluated for filaricidal activity against litomosoides carinii and acanthocheilonema viteae infections in cotton rats (sigmodon hispidus) and mastomys coucha respectively. significant effects on macrofilariae (>80% death/sterilisation) were detected with compounds #2, 3 and 6 against l. carinii and/or a. viteae. thus detection of filaricidal activity in benzopyrones, which are so far known fo ... | 2000 | 10936568 |
| effects of 2-deoxy-d-glucose on acanthocheilonema viteae: rodent filariids as studied by multinuclear nmr spectroscopyt. | a well known glucose antimetabolite, 2-deoxy glucose (2dg) widely used in chemotherapy of cancer along with radiation, was evaluated as an antifilarial agent by nuclear magnetic resonance. the uptake and metabolism of 2dg in the experimental filarial infection acanthocheilonema viteae was studied by in vivo multinuclear nmr. an unusually long retention time of 2dg6p within these parasites was observed on continuous 31p nmr monitoring, along with a decrease in atp levels. these results led to the ... | 2000 | 10970042 |
| setaria digitata infections in cattle: parasite load, microfilaraemia status and relationship to immune response. | a total of 110 cattle were examined in an area endemic for bancroftian filariasis for the prevalence of infection of the bovine filarial parasite setaria digitata. about 12.5% of cattle were found to harbour both adult worms in the peritoneum and microfilariae (mf) in circulation; 70% of the cattle were amicrofilaraemic but with an adult worm infection. a third group of cattle (16.5%) was free of detectable mf and adult worms. the presence of adult worms and/or mf did not influence the antibody ... | 2000 | 11138024 |
| experimentally induced infection with autonomous parvoviruses, minute virus of mice and h-1, in the african multimammate mouse (mastomys coucha). | to determine whether the multimammate mouse (mastomys coucha) could be used to evaluate rodent parvovirus-based vectors, neonates were subcutaneously inoculated with minute virus of mice (prototype strain, mvmp) or rat parvovirus h-1. the course of infection with both viruses was similar. seroconversion occurred within two weeks after virus inoculation, as detected by use of hemagglutination-inhibition assays, and antibody titers remained high for the entire observation period of 12 months. vira ... | 2000 | 11200567 |
| bone marrow response to acute and chronic trypanosoma congolense infection in multimammate rats (mastomys coucha). | the femoral bone marrow of multimammate rats (n=90), aged 3-8 weeks, experimentally infected with different doses of trypanosoma congolense was examined by light and electron microscopy. some animals died from trypanosomosis, but groups of 10 were killed at 4-8, 9-16, 20-24, 30, 40, 50 and 60 days post-infection (dpi). in the acute stage of infection (4-8 dpi) the bone marrow invariably showed a striking increase in erythropoiesis, characterized by an increase in the number of mitotic figures an ... | 2001 | 11222012 |
| effects of bay 44-4400, a new cyclodepsipeptide, on developing stages of filariae (acanthocheilonema viteae, brugia malayi, litomosoides sigmodontis) in the rodent mastomys coucha. | bay 44-4400 was used as a spot on formulation and administered in single doses of 25 and 100 mg/kg to acanthocheilonema viteae, brugia malayi, and litomosoides sigmodontis infected mastomys coucha on various dates during prepatency, aiming to affect third stage larvae, fourth stage larvae or preadult worms. microfilaraemia levels were controlled in comparison to untreated controls until necropsies were performed 100 days p.i. (a. viteae, l. sigmodontis) and 150 days p.i. (b. malayi) to determine ... | 2001 | 11495640 |
| filaricidal efficacy of anthelmintically active cyclodepsipeptides. | pf 1022a, a novel anthelmintically active cyclodepsipeptide, and bay 44-4400, a semisynthetic derivative of pf 1022a were tested for filaricidal efficacy in mastomys coucha infected with litomosoides sigmodontis, acanthocheilonema viteae and brugia malayi. the parent compound pf 1022a showed limited anti-filarial efficacy in l. sigmodontis and b. malayi infected animals. oral doses of 5 x 100 mg/kg on consecutive days caused only a temporary decrease of microfilariaemia levels. by contrast, bay ... | 2001 | 11595239 |
| distribution of trypanosoma congolense in infected multimammate rats (mastomys coucha): light and electron microscopical studies. | in an attempt to determine whether trypanosoma congolense occurs both within and outside the blood vessels in an infected animal host, multimammate rats (mastomys coucha) were infected with t. congolense and samples from spleen, lymph nodes, bone marrow, liver, kidney, lungs, brain, heart, intestines, ovaries and testes were collected. the tissue samples were fixed and processed for light and electron microscopical examination. in all the tissues examined, trypanosomes were found only within the ... | 2002 | 11983307 |
| fertilization of oocytes and birth of normal pups following intracytoplasmic injection with spermatids in mastomys (praomys coucha). | the mastomys is a small laboratory rodent that is native to africa. although it has been used for research concerning reproductive biology, in vitro fertilization (ivf) and intracytoplasmic sperm injection are very difficult in mastomys because of technical problems, such as inadequate sperm capacitation and large sperm heads. the present study was undertaken to examine whether mastomys spermatids could be used to fertilize oocytes in vitro using a microinsemination technique, because spermatids ... | 2003 | 12606361 |
| 7-o-[4-methyl piperazine-1-(2-acetyl)]-2h-1-benzopyran-2-one: a novel antifilarial lead compound. | in preliminary studies we found that benzopyrones (coumarins), which are known to exert many biological activities including anti-inflammatory effect, possess promising macrofilaricidal action as well. in order to explore the possibility of combining such a macrofilaricidal activity with the microfilaricidal potential of the known piperazine pharmacophore, we synthesized a series of compounds and evaluated their antifilarial effect. in the present study, one of these compounds, 7-o-[4-methyl pip ... | 2003 | 12826297 |
| [demonstration and frequency of bartonellae in the blood of the rodents of the upper ubangi pertaining to the following species; mylomys cuninnghamei alberti, epimus rattus frugivorus, rattus mastomys coucha, louphuromys silkapusi, dasymys incomitus fuscus and teaterillus emini]. | | 1953 | 13150077 |
| 4-methyl-7-(tetradecanoyl)-2h-1-benzopyran-2-one: a novel dna topoisomerase ii inhibitor with adulticidal and embryostatic activity against sub-periodic brugia malayi. | a compound of the coumarin class, 4-methyl-7-(tetradecanoyl)-2h-1-benzopyran-2-one, was evaluated for antifilarial activity against the human filarial parasite, brugia malayi (sub-periodic strain) in mastomys coucha. the test compound brought about a 24.4% reduction in circulating microfilaremia on day 8 after initiation of treatment when administered by the peritoneal route at a dose of 50 mg/kg for 5 consecutive days. the compound also caused a 62.0% mortality in adult parasites. apart from ki ... | 2004 | 14652743 |
| the antifilarial activity of a marine red alga, botryocladia leptopoda, against experimental infections with animal and human filariae. | the antifilarial activity of the marine red alga botryocladia leptopoda against rodent and human lymphatic filarial parasites is described. the animal filarial species included litomosoides sigmodontis and acanthocheilonema viteae maintained in cotton rats and mastomys coucha, respectively, while a subperiodic strain of the human lymphatic filarial parasite brugia malayi was maintained in m. coucha. the crude extract and its hexane fraction brought about a marked reduction in the peripheral micr ... | 2004 | 15243801 |
| antifilarial activity of zoanthus species (phylum coelenterata, class anthzoa) against human lymphatic filaria, brugia malayi. | the chloroform methanol (1:1) extract of an unidentified green zoanthus (phylum coelenterata, class anthozoa) showed promising in vitro adulticidal activity with a lethal concentration of 125 microg/ml on brugia malayi. this extract brought about a 52.2% reduction in circulating microfilariae of b. malayi when administered to infected mastomys coucha at 250 mg/kg, orally for 5 consecutive days. further fractionation of the extract led to the recovery of four fractions, which were evaluated simul ... | 2004 | 15258853 |
| sequence analysis of cdna encoding follicle-stimulating hormone and luteinizing hormone beta-subunits in the mastomys (praomys coucha). | to examine the molecular basis of efficient superovulation in the mastomys (praomys coucha), the cdna sequences of the follicle-stimulating hormone (fsh) and luteinizing hormone (lh) beta-subunits were determined and compared with those of other mammals. fshbeta and lhbeta cdnas were 1606 and 513 bp long, respectively, from the 5'-ends to the putative polya sites. the deduced sequences of the fshbeta and lhbeta precursor proteins were 130 and 141 amino acids in length, respectively. the amino ac ... | 2004 | 15364211 |
| responses of mastomys coucha, that have been infected with brugia malayi and treated with diethylcarbamazine or albendazole, to re-exposure to infection. | the responses of mastomys coucha to re-exposure to infection with homologous infective larvae (l(3)) of brugia malayi were investigated, after initial infections with the nematode had been treated subcutaneously for 5 days with diethylcarbamazine (dec; 150 mg citrate/kg. day) or albendazole (alb; 50 mg/kg. day). the parasite burdens, serum concentrations of igg reacting with a soluble somatic extract of adult b. malayi (bmas), and cytokine and lymphocyte-proliferative responses to filarial antig ... | 2004 | 15667714 |
| inflammatory antigens of brugia malayi and their effect on rodent host mastomys coucha. | the study was aimed at identifying pro- and anti-inflammatory cytokine releasing potential of brugia malayi adult worm fractions and their role in filarial infection and pathogenesis. thp-1 cells were incubated with soluble somatic brugia malayi adult worm extract (bmas) and its sephadex g-200 fractions bmafi, bmafii and bmafiii and the effect of the fractions on parasitological, immunological and lymph node parameters was assessed in mastomys coucha. bmafii stimulated the pro-inflammatory tnf-a ... | 2004 | 15752117 |
| lethal lps-independent side effects after microfilaricidal treatment in acanthocheilonema viteae-infected rodents. | mastomys coucha and jirds infected with acanthocheilonema viteae, a filarial species free of endosymbiontic bacteria of the genus wolbachia, suffer lethal side effects after effective microfilaricidal therapy with diethylcarbamazine and levamisole, whereas, m. coucha infected with the wolbachia-infested species brugia malayi or litomosoides carinii tolerate corresponding treatment. mortality in a. viteae infected, treated animals varied with microfilariae density in the blood. it was up to 100% ... | 2005 | 15997410 |
| acanthocheilonema viteae in mastomys coucha: chemotherapeutic and chemoprophylactic role of vitamin a in experimental filarial infection. | the role of vitamin a was evaluated for its chemotherapeutic and chemoprophylactic action against acanthocheilonema viteae infection in mastomys coucha. vitamin a was administered for 10 days, five days before infection and five days post infection. on day 0 experimental animals as well as controls were infected with l3, the infective stage. establishment of the worms revealed significantly less percentage of worm recovery over untreated controls. cell-mediated response was found to be the cause ... | 2006 | 16729591 |
| protection against l3 induced brugia malayi infection in mastomys coucha pre-immunized with bmafii fraction of the filarial adult worm. | the present study was aimed at investigating protective efficacy of bmafii (sephadex g-200 eluted fraction of brugia malayi adult worm extract) against establishment of infective larvae (l3)-induced b. malayi infection in mastomys coucha and to delineate immunological responses induced in the host. healthy male m. coucha were immunized with bmafii and subsequently inoculated with b. malayi l3. specific igg and cell mediated immune responses (cellular proliferation) including release of tumor nec ... | 2006 | 16757067 |
| localization of brugia malayi (sub-periodic) adults in different organs of mastomys coucha and its influence on microfilaraemia and host antibody response. | lymphatic filariasis caused by nematode parasites wuchereria bancrofti or brugia malayi is a spectral disease and produces wide range of immune responses and varying levels of microfilaraemia in infected individuals. the relationship between the immune response of host and the developmental stage of the parasite as well as the microfilariae (mf) density and specific location of the adult worms is yet to be understood. as an experimental model, b. malayi adapted in the experimental animal mastomy ... | 2006 | 16862320 |
| ultrasonography in filaria-infected rodents: detection of adult litomosoides sigmodontis and brugia malayi filariae. | to evaluate the usefulness of ultrasonography (usg) in the detection of adult filariae in rodents. wuchereria bancrofti are frequently detected using usg in humans, whereas adult brugia malayi have not been so far. | 2006 | 16930260 |
| infectivity and pathogenicity of cryptosporidium andersoni to a novel host, southern multimammate mouse (mastomys coucha). | the infectivity and pathogenicity of cryptosporidium andersoni (bovine isolate) for neonatal and adult southern multimammate mice (mastomys coucha) was studied using transmission experiments. c. andersoni isolate used in this study was not infective for balb/c mice, but experimental infection proved susceptibility of neonatal and adult m. coucha to the infection. the prepatent period was 20-24 days, the patent period varied between 46 and 59 days. no signs of clinical illness or macroscopic find ... | 2007 | 16997473 |
| chemical constituents and antifilarial activity of lantana camara against human lymphatic filariid brugia malayi and rodent filariid acanthocheilonema viteae maintained in rodent hosts. | lymphatic filariasis continues to be a major health problem in tropical and subtropical countries. a macrofilaricidal agent capable of eliminating adult filarial parasites is urgently needed. in the present study, we report the antifilarial activity in the extract of stem portion of the plant lantana camara. the crude extract at 1 g/kg for 5 days by oral route killed 43.05% of the adult brugia malayi parasites and sterilized 76% of surviving female worms in the rodent model mastomys coucha. a 34 ... | 2007 | 17061115 |
| anti-filarial activity of novel formulations of albendazole against experimental brugian filariasis. | the study was aimed at developing better orally active albendazole (alb) formulations. six formulations (alb-1 to alb-6) were prepared and tested against brugia malayi in mastomys coucha and jird (meriones unguiculatus) at 200 mg/kg, orally, for 5 consecutive days. the anti-filarial efficacy was assessed against microfilariae (mf), adult worms and female reproductive potential. three of the 6 alb formulations showed greatly improved female worm sterilizing potential (alb-1: 90%; alb-3: 63%; alb- ... | 2007 | 17078904 |
| skin nodules on multimammate mice (mastomys coucha). | | 2007 | 17721527 |
| untangling the roles of fire, grazing and rainfall on small mammal communities in grassland ecosystems. | in grassland systems across the globe, ecologists have been attempting to understand the complex role of fire, grazing and rainfall in creating habitat heterogeneity and the consequences of anthropogenic control of these factors on ecosystem integrity and functioning. using a south african grassland ecosystem as a model, we investigated the impact of fire and grazing pressure on small mammal communities during three differing periods of a rainfall cycle. over 2 years, 15,203 trap nights revealed ... | 2007 | 17846799 |
| persistence of mastomys natalensis papillomavirus in multiple organs identifies novel targets for infection. | the high incidence of multiple wart formation and skin cancer in organ-transplant recipients, as well as the question of an involvement of papillomaviruses in a variety of human cancers, require a model system for papillomavirus infections in immunocompetent animals. such an in vivo model is represented by the multimammate rat mastomys coucha, which is infected with mastomys natalensis papillomavirus (mnpv). mnpv primarily induces benign skin tumours, such as papillomas and keratoacanthomas. her ... | 2007 | 17872518 |
| monoclonal antibodies generated against excretory/secretory antigens of mammalian stage larvae of the lymphatic filarial parasite wuchereria bancrofti. | monoclonal antibodies (mabs) against excretory/secretary (e/s) antigens of fourth stage (l4) larvae of wuchereria bancrofti were raised and screened for their specificity and sensitivity and evaluated for their potential in detecting homologous e/s antigens in human blood samples. five mabs were obtained and, among them, mab a7 showed high reactivity against e/s antigens of l4 and crude somatic antigens of microfilariae (mf) of w. bancrofti, and infective stage (l3) and adult stage larvae of bru ... | 2007 | 17885888 |
| prior killing of intracellular bacteria wolbachia reduces inflammatory reactions and improves antifilarial efficacy of diethylcarbamazine in rodent model of brugia malayi. | the discovery of the endosymbiont wolbachia, which has a mutualistic relationship with filarial nematodes, and its importance in filarial parasite biology has provided a lead for developing novel chemotherapeutic agents against human filariasis. wolbachia also appears to be involved in immunopathological responses as well as adverse reactions after antifilarial therapy. the aim of the present study was to explore the potential of administering anti-wolbachial therapy before antifilarial treatmen ... | 2008 | 18180957 |
| effect of chemotherapeutic treatment on cytokine (ifn-gamma, il-2, il-4, il-5, il-10) gene transcription in response to specific antigens in brugia malayi-infected mastomys coucha. | cytokine (interferon (ifn)-gamma, interleukin (il)-2, il-4, il-5, il-10) gene transcription in response to filarial antigens was determined in peripheral blood mononuclear cells of brugia malayi-infected mastomys coucha in the course of untreated and chemotherapeutically abbreviated infections. transcript levels in infected untreated animals suggest particular time courses for the various cytokines with ongoing parasite development and differing efficacies of female, male, microfilarial, and l3 ... | 2008 | 18679719 |
| antifilarial activity of caesalpinia bonducella against experimental filarial infections. | lymphatic filariasis is a disabling disease that continues to cripple population in tropical countries. currently available antifilarial drugs are not able to control the disease. therefore, a better antifilarial is urgently required for proper management of the disease. we undertook this study to assess the antifilarial activity of caesalpinia bonducella-seed kernel against rodent filarial parasite in experimental model. | 2008 | 18820361 |
| antifilarial lead molecules isolated from trachyspermum ammi. | lymphatic filariasis is caused by infection with the parasitic filarial nematodes wuchereria bancrofti, brugia malayi and b. timori, transmitted by mosquitoes. the lack of an adulticidal drug poses a challenge to filariasis elimination, hence it is essential to develop an effective antifilarial drug which could either kill or permanently sterilize the adult worms. in the reported work the in vitro activity of a methanolic extract of fruits of trachyspermum ammi (apiaceae) against adult bovine fi ... | 2008 | 18830147 |
| protective efficacy of a filarial surface antigen in experimental filariasis. | a water-insoluble, detergent-soluble, surface-associated glycoprotein, designated as dssd1, was found to induce microfilaria clearance in mastomys coucha implanted with setaria digitata. intraperitoneal implantation of adult female worms of s. digitata in m. coucha could induce microfilaraemia lasting about 165 days in circulation. immunization of m. coucha with dssd1 antigen either before or after implantation of worms resulted in a significant reduction in microfilaria density. complete cleara ... | 2009 | 18922206 |
| amplification of rat microsatellite loci in mastomys coucha smith, 1836. | the multimammate rat m. coucha is the most widespread strain to be introduced in biomedical research and various stocks of this strain are maintained in laboratories across the globe. it is an ideal carrier of normally non-human disease to the domestic environment. in order to analyze genetic purity, strains of m. coucha were subjected to pcr-based dna fingerprinting using sequence tagged microsatellite markers to evolve molecular signature to them. for this, 10 rats sequenced tagged microsatell ... | 2008 | 18949891 |
| adult brugia malayi mitochondrial and nuclear fractions impart th1-associated sizeable protection against infective larval challenges in mastomys coucha. | protective immunity to the subperiodic human filariid, brugia malayi, was explored in the rodent host, mastomys coucha after vaccination with subcellular fractions derived from the adult stage of the parasite. the highest level of protection was conferred in animals vaccinated with the 'mitochondria rich' (mt) fraction, in which microfilaraemia and worm burden were markedly reduced by 67.2 and 65.9%, respectively, followed by the 'nucleus rich' (nr) fraction, showing reductions of 62 and 52.3%, ... | 2009 | 19063769 |
| the n-hexane and chloroform fractions of piper betle l. trigger different arms of immune responses in balb/c mice and exhibit antifilarial activity against human lymphatic filarid brugia malayi. | modulation of immune functions by using herbal plants and their products has become fundamental regime of therapeutic approach. piper betle linn. (piperaceae) is a widely distributed plant in the tropical and subtropical regions of the world and has been attributed as traditional herbal remedy for many diseases. we have recently reported the antifilarial and antileishmanial efficacy in the leaf extract of bangla mahoba landrace of p. betle which is a female plant. the present report describes th ... | 2009 | 19281872 |
| immunization with inflammatory proteome of brugia malayi adult worm induces a th1/th2-immune response and confers protection against the filarial infection. | mastomys coucha and jirds (meriones unguiculatus) were immunized with four cytokine-stimulating sds-page resolved fractions f5 (68-84 kda), f6 (54-68 kda), f10 (38-42 kda) and f14 (20-28 kda) of brugia malayi adult worm to determine which of these fractions has the potential to influence the establishment of subsequently introduced b. malayi infection in the animals. the proteins in the fractions were analyzed by 2de and maldi-tof. immunization with f6 suppressed the establishment of third stage ... | 2009 | 19450648 |
| in vitro and in vivo antifilarial potential of marine sponge, haliclona exigua (kirkpatrick), against human lymphatic filarial parasite brugia malayi: antifilarial activity of h. exigua. | the present study reports on the antifilarial activity of a marine sponge haliclona exigua (phylum porifera). the crude methanol extract and n-butanol-soluble fraction killed adult brugia malayi at 31.25-microg/ml concentration (both in motility and 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay) while the chloroform fraction was lethal at a lower concentration of 15.6 microg/ml. the activity could be located in a single molecule araguspongin c which brought about mortality ... | 2009 | 19626340 |
| adult brugia malayi approximately 34 kda (bmt-5) antigen offers th1 mediated significant protection against infective larval challenge in mastomys coucha. | we earlier reported a sizeable protection conferred by 'mitochondria rich' (mt) fraction of adult b. malayi and the present study was planned to locate the candidate protective molecule/s in the active fraction. the mt fraction was subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and the antigen bands showing strong immune-reactivity with the resistant mastomys sera were assayed for their lymphoproliferative response using splenocytes of protected animals. of th ... | 2009 | 19638321 |
| improvement in the antifilarial efficacy of doxycycline and rifampicin by combination therapy and drug delivery approach. | the present investigation deals with the evaluation of antifilarial efficacy of liposome entrapped antiwolbachial antibiotics doxycycline and rifampicin (5 doses at 10 mg/kg, subcutaneously for 15 days) alone and/or in combination with standard filaricide diethylcarbamazine (dec) against human lymphatic filariid brugia malayi in rodent host mastomys coucha. the delivery system maintained the sustained release of antibiotics up to 48 h and significantly (p < 0.05) augmented the antifilarial poten ... | 2010 | 19954408 |
| antifilarial activity in vitro and in vivo of some flavonoids tested against brugia malayi. | we evaluated the antifilarial activity of 6 flavonoids against the human lymphatic filarial parasite brugia malayi using an in vitro motility assay with adult worms and microfilariae, a biochemical test for viability (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (mtt)-reduction assay), and two animal models, meriones unguiculatus (implanted adult worms) and mastomys coucha (natural infections). in vitro, naringenin and hesperetin killed the adult worms and inhibited (>60%) mtt-re ... | 2010 | 20609356 |
| genomic analysis of the first laboratory-mouse papillomavirus. | a papillomavirus (pv) that naturally infects laboratory mice will provide an extremely valuable tool for pv research. we describe here the isolation, cloning and molecular analysis of the first novel laboratory-mouse pv, designated muspv. this agent, recently identified in the tissues from florid and asymmetrical papillomas on the face of nude mice (nmri-foxn1(nu)/foxn1(nu)), was demonstrated to be transmissible to immunocompetent mice (ingle et al., 2010). the muspv genome is 7510 bp in length, ... | 2010 | 21084500 |
| gedunin and photogedunin of xylocarpus granatum possess antifilarial activity against human lymphatic filarial parasite brugia malayi in experimental rodent host. | the present study is aimed to evaluate antifilarial activity of xylocarpus granatum (fruit from andaman) against human lymphatic filarial parasite brugia malayi in vivo. the in vitro antifilarial activity has already been reported earlier for this mangrove plant which has traditionally been used against several ailments. aqueous ethanolic crude extract, four fractions (ethyl acetate fraction, n-butanol fraction, water-soluble fraction and water-insoluble fraction) and pure molecule/s of x. grana ... | 2011 | 21523424 |
| inflammatory mediator release by brugia malayi from macrophages of susceptible host mastomys coucha and thp-1 and raw 264.7 cell lines. | to investigate which life stage of the parasite has the ability to stimulate release of pro- or anti-inflammatory mediators from macrophages. | 2011 | 21771428 |
| Sensitization with anti-inflammatory BmAFI of Brugia malayi allows L3 development in the hostile peritoneal cavity of Mastomys coucha. | Filarial parasites survive by inducing tolerance in host but the antigens and mechanisms involved are not clear. Recently we found that BmAFI, a Sephadex G-200 eluted fraction of Brugia malayi adult worm extract, stimulates IL-10 release from THP-1 cells. In the present study, we determined the SDS-PAGE profile of BmAFI and infective 3rd stage larva (L3), investigated the effect of pre-sensitization of host with BmAFI on the survival and development of L3 in the non-permissive peritoneal cavity ... | 2011 | 21875568 |
| The adjuvant-free immunoprotection of recombinant filarial protein Abundant Larval Transcript-2 (ALT-2) in Mastomys coucha and the immunoprophylactic importance of its putative signal sequence. | The filarial protein Abundant Larval Transcript-2 (ALT-2) of the filarial parasite Brugia malayi has been shown to produce 74% worm clearance when administered with an adjuvant. In the present study, we show that it not only induces humoral and cell-mediated immunity, but also protection up to 71% in Mastomys coucha, a permissive animal model for filariasis, even without adjuvant. This unique feature of ALT-2 protein is highly restricted to its 21 amino acid N-terminal signal sequence, the absen ... | 2011 | 21864531 |
| immunization with brugia malayi myosin as heterologous dna prime protein boost induces protective immunity against b. malayi infection in mastomys coucha. | the current control strategies employing chemotherapy with diethylcarbamazine, ivermectin and albendazole have reduced transmission in some filaria-endemic areas, there is growing interest for complementary approaches, such as vaccines especially in light of threat of parasite developing resistance to mainstay drugs. we earlier demonstrated recombinant heavy chain myosin of b. malayi (bm-myo) as a potent vaccine candidate whose efficacy was enhanced by heterologous dna prime/protein boost (myo-p ... | 2016 | 27828973 |
| antifilarial activity of diterpenoids from taxodium distichum. | lymphatic filariasis caused by wuchereria bancrofti, brugia malayi and b. timori, is a debilitating disease with an adverse social and economic impact. the infection remains unabated in spite of treatment with existing antifilarial drugs diethylcarbamazine (dec) and ivermectin which are chiefly microfilaricides. there is therefore, need for macrofilaricides, embryostatic agents and better microfilaricides. in the present study we explored the antifilarial potential of crude extract and its molec ... | 2016 | 27245322 |
| cofactor independent phosphoglycerate mutase of brugia malayi induces a mixed th1/th2 type immune response and inhibits larval development in the host. | lymphatic filariasis is a major debilitating disease, endemic in 72 countries putting more than 1.39 billion people at risk and 120 million are already infected. despite the significant progress in chemotherapeutic advancements, there is still need for other measures like development of an effective vaccine or discovery of novel drug targets. in this study, structural and immunological characterization of independent phosphoglycerate mutase of filarial parasite brugia malayi was carried out. pro ... | 2014 | 25061608 |
| cryptosporidium scrofarum n. sp. (apicomplexa: cryptosporidiidae) in domestic pigs (sus scrofa). | we describe the morphological, biological, and molecular characteristics of cryptosporidium pig genotype ii and propose the species name cryptosporidium scrofarum n. sp. to reflect its prevalence in adult pigs worldwide. oocysts of c. scrofarum are morphologically indistinguishable from c. parvum, measuring 4.81-5.96 μm (mean=5.16)×4.23-5.29 μm (mean=4.83) with a length to width ratio of 1.07±0.06 (n=400). oocysts of c. scrofarum obtained from a naturally infected pig were infectious for 8-week- ... | 2012 | 23021264 |
| cryptosporidium proliferans n. sp. (apicomplexa: cryptosporidiidae): molecular and biological evidence of cryptic species within gastric cryptosporidium of mammals. | the morphological, biological, and molecular characteristics of cryptosporidium muris strain ts03 are described, and the species name cryptosporidium proliferans n. sp. is proposed. cryptosporidium proliferans obtained from a naturally infected east african mole rat (tachyoryctes splendens) in kenya was propagated under laboratory conditions in rodents (scid mice and southern multimammate mice, mastomys coucha) and used in experiments to examine oocyst morphology and transmission. dna from the p ... | 2016 | 26771460 |
| disorganized muscle protein-1 (dim-1) of filarial parasite brugia malayi: cdna cloning, expression, purification, structural modeling and its potential as vaccine candidate for human filarial infection. | we have recently identified disorganized muscle protein-1 (dim-1) in one of the proinflammatory fractions of the human filaria brugia malayi adult worm. the present study was undertaken to characterize b. malayi dim-1 (dim-1bm) and explore its vaccine potential. in this study we cloned and expressed the dim-1bm gene, investigated its sequence homology with other nematodes, constructed in silico structural model, purified the recombinant dim-1bm (rdim-1bm) protein, and studied the effect of immun ... | 2014 | 24513011 |
| subcutaneously administered ultrafine plga nanoparticles containing doxycycline hydrochloride target lymphatic filarial parasites. | systemic chemotherapeutic targeting of filarial parasites is unfocused due to their deep seated location in lymphatic vessels. this warrants a prolonged dosing regimen in high doses for an anthelmintic like doxycycline hydrochloride (dox). in order to provide an alternative, we have constructed ultrafine plga nanoparticles of dox (dpnps), so as to exploit the peculiarity of lymphatic vasculature underneath the subcutaneous layer of skin, which preferentially allows entry of only 10-100 nm sized ... | 2016 | 27144397 |
| epitope mapping of brugia malayi alt-2 and the development of a multi-epitope vaccine for lymphatic filariasis. | human lymphatic filariasis is a neglected tropical disease, causing permanent and long-term disability with severe immunopathology. abundant larval transcript (alt) plays a crucial role in parasite establishment in the host, due to its multi-faceted ability in host immune regulation. although alt protein is a key filarial target, its exact function is yet to be explored. here, we report epitope mapping and a structural model of brugia malayi alt-2, leading to development of a multi-epitope vacci ... | 2017 | 26892175 |
| moxidectin causes adult worm mortality of human lymphatic filarial parasite brugia malayi in rodent models. | moxidectin is a macrocyclic lactone belonging to milbemycin family closely related to ivermectin and is currently progressing towards phase iii clinical trial against human infection with the filaria onchocerca volvulus (leuckart, 1894). there is a single report on the microfilaricidal and embryostatic activity of moxidectin in case of the human lymphatic filarial parasite brugia malayi (brug, 1927) in mastomys coucha (smith) but without any adulticidal action. in the present study, the in vitro ... | 2014 | 25651699 |
| protection against filarial infection by 45-49 kda molecules of brugia malayi via ifn-γ-mediated inos induction. | nitric oxide (no) mediated mechanisms have been implicated in killing of some life-stages of brugia malayi/wuchereria bancrofti and protect the host through type 1 responses and ifn-γ stimulated toxic mediators' release. however, the identity of no stimulating molecules of the parasites is not known. three predominantly no-stimulating sds-page resolved fractions f8 (45.24-48.64 kda), f11 (33.44-38.44 kda) and f12 (28.44-33.44 kda) from b. malayi were identified and their proteins were analyzed b ... | 2015 | 25454090 |
| synthesis and antifilarial activity of chalcone-thiazole derivatives against a human lymphatic filarial parasite, brugia malayi. | here we report the synthesis of novel chalcone-thiazole compounds and their antifilarial activity. the antifilarial properties of these hybrids were assessed against microfilariae as well as adult worms of brugia malayi. among all the synthesized compounds, only two compounds, namely 4g and 4n were identified to be promising in vitro. these active compounds were tested in b. malayi-jird (meriones unguiculatus) and b. malayi-mastomys coucha models. compound 4n showed 100% embryostatic effect and ... | 2014 | 24863844 |
| nanocurcumin: a novel antifilarial agent with dna topoisomerase ii inhibitory activity. | the aim of this study is to evaluate the antifilarial, antiwolbachial and dna topoisomerase ii inhibitory activity of nanocurcumin (nano-cur). | 2014 | 24479705 |
| therapeutic efficacy of poly (lactic-co-glycolic acid) nanoparticles encapsulated ivermectin (nano-ivermectin) against brugian filariasis in experimental rodent model. | the present study reports on the antifilarial activity of poly (lactic-co-glycolic acid) nanoparticles encapsulated ivermectin (nano-ivm) against human lymphatic filariid brugia malayi in rodent host mastomys coucha. nano-ivm was prepared and optimized by nanoprecipitation method. the selected nano-ivm (f5) showed a uniform spherical shape with 96 nm diameter and 74.12 % entrapment efficiency, and when used at a suboptimal dose of 100 μg/kg body weight, completely eliminated filarial parasites f ... | 2014 | 24366812 |
| immunization of mastomys coucha with brugia malayi recombinant trehalose-6-phosphate phosphatase results in significant protection against homologous challenge infection. | development of a vaccine to prevent or reduce parasite development in lymphatic filariasis would be a complementary approach to existing chemotherapeutic tools. trehalose-6-phosphate phosphatase of brugia malayi (bm-tpp) represents an attractive vaccine target due to its absence in mammals, prevalence in the major life stages of the parasite and immunoreactivity with human bancroftian antibodies, especially from endemic normal subjects. we have recently reported on the cloning, expression, purif ... | 2013 | 24015262 |
| improved antifilarial activity of ivermectin in chitosan-alginate nanoparticles against human lymphatic filarial parasite, brugia malayi. | the current antifilarial treatments are not up to the mark partly due to deep location of filarial parasites in the human lymphatic system. we report here on the improvement in the antifilarial activity of ivermectin (ivm) using chitosan-alginate nanoparticles prepared by modified complex coacervation method. the nanoparticles were spherical having 155 nm size and 4.56 and 75.67% loading and entrapment efficiency respectively for ivm. the delivery system maintained the sustained release and sign ... | 2013 | 23828187 |