| anticholinergic poisoning due to chinese herbal medicines. | serious poisoning may occur following the consumption of chinese herbal medicines (chm) containing anticholinergics. the great majority of cases are probably related to the use of yangjinhua, the dried flower of datura metel l, for treating bronchial asthma, chronic bronchitis, pains and flu symptoms. in some cases the use of chm contaminated by atropine-like substances or fake herbs were suspected. some ginseng (panax ginseng) preparations might have been adulterated with mandragora officinarum ... | 1995 | 7631497 |
| effect of withafastuosin e on gastric mucosal offensive and defensive factors in rats. | withafastuosin e (we), a withanolide of datura fastuosa, has been reported to possess anti-stress activity and augment prostaglandins. the present investigation has been undertaken to evaluate the anti-ulcer activity and its mechanism in various models of experimentally induced ulcers in rats. we (20 mg/kg, po) reduced the incidence of ulcer and ulcer index significantly in rats. the drug also decreased volume of gastric secretion, acid and peptic output, though it did not affect mucin secretion ... | 1997 | 9418376 |
| datura metel: in vitro production of tropane alkaloids. | hairy root lines of datura metel were established following infection of aseptic stem segments with agrobacterium rhizogenes strain a4 and cultured in hormone-free b5 solid medium. the growth and production of hyoscyamine and scopolamine (mg/g dry wt.) of these root cultures was encouraged by using b5 liquid medium with half-strength salts. in these culture conditions, the capacity of the highest productive root line 25 to excrete scopolamine into the culture medium rose from 8.7% to 70% when th ... | 1999 | 10193206 |
| studies on antimycotic properties of datura metel. | the hexane, chloroform, acetone and methanolic fractions of datura metel l. were investigated for antifungal properties using pathogenic species of aspergillus. the chloroform fraction was found to be endowed with antifungal activity. the minimum inhibitory concentration (mic) of chloroform fraction of d. metel l. was 625.0 microg ml(-1) against all the three species of aspergillus, i.e. a. fumigatus, a. flavus and a. niger, by microbroth dilution and percent spore germination inhibition assays. ... | 2002 | 12007710 |
| effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of datura metel and hyoscyamus muticus. | in order to increase the production of the pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed to introduce the t-dna of the ri plasmid together with the tobacco pmt gene under the control of camv 35s promoter, into the genome of datura metel and hyoscyamus muticus. this gene codes for putrescine:sam n-methyltransferase (pmt; ec. 2.1.1.53), which catalyses the first committed step in the tropane alkaloid pathway. hairy root cultures overexpres ... | 2003 | 12493848 |
| different elongation pathways in the biosynthesis of acyl groups of trichome exudate sugar esters from various solanaceous plants. | two common pathways are known for elongation of aliphatic acids via acetate in biological organisms: the fatty acid synthase (fas) and the alpha-ketoacid elongation (alphakae) pathways. the alphakae route is utilized in many biosynthetic pathways, including the tricarboxylic acid cycle, leucine biosynthesis, and in formation of coenzyme b, glucosinolates, alpha-ketoadipate, sugar-ester acyl acids, short-chain alcohols of yeast and clostridium species, 2-amino-4-methylhex-4-enoic acid, and l-gamm ... | 2003 | 12687369 |
| evaluation of hypoglycemic and antihyperglycemic effects of datura metel (linn.) seeds in normal and alloxan-induced diabetic rats. | the seed powder of datura metel was tested for its hypoglycemic activity in normal and alloxan-induced diabetic rats. graded doses (25, 50 and 75 mg/kg, p.o.) of the seed powder when given to both normal and diabetic rats produced significant reduction in blood glucose at the 8 h. the effect was found to be dose dependent with all treatments at the doses administered. | 2004 | 15036475 |
| antifungal potential of indian medicinal plants. | fourteen indian plants, selected based on their use in respiratory and other disorders in traditional systems of medicine, were analyzed for their potential activity against fungi. the antifungal activity was investigated by disc diffusion, microbroth dilution and percent spore germination inhibition tests against pathogenic aspergilli. methanolic extracts of solanum xanthocarpum and datura metel inhibited the growth of aspergillus fumigatus, a. flavus and a. niger and their in vitro mics were f ... | 2004 | 15159003 |
| a novel antifungal pyrrole derivative from datura metel leaves. | phytochemical investigation of the leaves of datura metel linn. led to the isolation of a new pyrrole derivative 1 which was characterised as 2beta-(3,4-dimethyl-2,5-dihydro-1h-pyrrol-2-yl)-1'-methylethyl pentanoate on the basis of spectral data analyses and chemical reactions. compound 1 was endowed with antifungal activity and its mic was found to be 87.5 microg/ml. two proteins having molecular weights of 42 and 58 kd of aspergillus fumigatus are potential targets for compound 1. | 2004 | 15296098 |
| [tmv-infection localization and development of induced virus resistance in nicotiana sanderae hort., datura stramonium l. and datura metel]. | the localization of virus infection and development of local and systemic induced resistance in plants nicotiana sanderae hort., datura stramonium l. and d. metel l. infected by tmv have been studied. it was shown that acquired resistance to the second infection is induced in hypersensitive plants infected by tmv independent of localization mechanism. no definite correlation exists between these defense reactions. | 2004 | 15515900 |
| in vitro antifungal activity of 2-(3,4-dimethyl-2,5-dihydro-1h-pyrrol-2-yl)-1-methylethyl pentanoate, a dihydropyrrole derivative. | a novel compound 2-(3,4-dimethyl-2,5-dihydro-1h-pyrrol-2-yl)-1-methylethyl pentanoate was isolated from the plant datura metel l. the in vitro activity of this dihydropyrrole derivative against aspergillus and candida species was evaluated by using standard methods approved by the national committee for clinical laboratory standards. the compound was found to be active against all the species tested, namely candida albicans, candida tropicalis, aspergillus fumigatus, aspergillus flavus and asper ... | 2005 | 15888463 |
| study on potato virus m (pvm) occurrence in potato fields in iran. | 57 native potato tuber samples collected from different potato growing region of iran, planted on single rows in karaj college experimental station. plant samples of each single row plus 9.25 fresh foliage samples collected from fields under new introduced cultivars were tested for potato virus (pvm) infection during growing season. also 78 weeds and field crops belonging to solonacae and leguminosae from or neighboring to potato field were tested. results indicated that pvm was not found on any ... | 2005 | 16637213 |
| xanthine oxidase inhibitory activity of some indian medical plants. | xanthine oxidase inhibitory activity was assayed from six species belonging to different families traditionally used for the treatment of gout and related symptoms by indigenous people of india. the aqueous, methanol-water mixture and methanolic extract of these plants were used for the experiment. of the 18 extracts assayed, 14 extracts demonstrated xanthine oxidase inhibitory activity at 100 microg/ml, among which 10 extracts showed an inhibition greater than 50% and ic(50) values below 100 mi ... | 2007 | 17014977 |
| daturametelins h, i, and j: three new withanolide glycosides from datura metel l. | three new withanolide glycosides named daturametelins h-j (1-3), together with two known ones, daturataturin a (4) and 7,27-dihydroxy-1-oxowitha-2,5,24-trienolide (5), were isolated from the meoh extract of the aerial parts of datura metel l. (solanaceae). their structures were determined mainly by spectroscopic techniques including 2d-nmr (hmbc, hmqc, (1)h,(1)h-cosy, noesy) and ms experiments. compounds 1-5 were tested for their antiproliferative activity towards the human colorectal carcinoma ... | 2006 | 17193256 |
| potato virus yisolated from pepper fields in tehran province. | potato virus y was known as the main cause of yellowing and vein necrosis of pepper in tehran province, using double antibody sandwich elisa (das-elisa). biological properties including host range of the isolate was determined after biological purification. host range studies showed that pepper isolate of pvy caused vein clearing and mosaic symptoms on datura metel and capsicum annum, mosaic on nicotiana tabacum cv. white barley, n. tabacum cv. samsun and n. rustica but didn't show any symptoms ... | 2006 | 17390898 |
| impacts of t-phylloplanin gene knockdown and of helianthus and datura phylloplanins on peronospora tabacina spore germination and disease potential. | t-phylloplanin proteins secreted to aerial surfaces of tobacco (nicotiana tabacum) by short procumbent trichomes inhibit spore germination and blue mold disease caused by the oomycete pathogen peronospora tabacina. many other plants were found to contain water-washed leaf surface proteins (phylloplanins), but the functions and properties of these are not known. here we extend earlier evidence for the antifungal activity of t-phylloplanins using a reverse genetics approach. rna interference of th ... | 2007 | 17573541 |
| toxicity studies on datura metel l. with reference to official stramonium. | suspensions of the powdered leaf of datura metel l. and datura stramonium l. (solanaceae) were administered by intubation at different doses: 0.125 mg/kg, 0.250 mg/kg, 0.500 mg/kg, 1.000 mg/kg, 1.224 mg/kg and 1.400 mg/kg to virgin female albino mice and were observed daily. after two weeks, the mice were sacrificed and the liver, kidney and intestine were removed, preserved in 10% formalin solution and embedded in paraffin wax. tissues from these organs were stained for assessment of tissue mor ... | 2007 | 17594983 |
| preventing establishment: an inventory of introduced plants in puerto villamil, isabela island, galapagos. | as part of an island-wide project to identify and eradicate potentially invasive plant species before they become established, a program of inventories is being carried out in the urban and agricultural zones of the four inhabited islands in galapagos. this study reports the results of the inventory from puerto villamil, a coastal village representing the urban zone of isabela island. we visited all 1193 village properties to record the presence of the introduced plants. in addition, information ... | 2007 | 17940606 |
| analysis of the biological and molecular variability of watermelon mosaic virus isolates from iran. | watermelon mosaic virus (wmv) is one of the most important viruses that causes different symptoms in cucurbitaceae. wmv is a potyvirus with a worldwide distribution, but occurs most commonly in temperate and mediterranean regions. cucurbit species grown in yazd, esfahan, west azerbaijan, hormozgan, and kerman provinces were surveyed for the relative incidence of wmv in 2004-2005. a total of 757 symptomatic cucurbit and 31 weed species were collected and assayed for infection with wmv. of 788 lea ... | 2008 | 18712590 |
| transcriptomic and reverse genetic analyses of branched-chain fatty acid and acyl sugar production in solanum pennellii and nicotiana benthamiana. | acyl sugars containing branched-chain fatty acids (bcfas) are exuded by glandular trichomes of many species in solanaceae, having an important defensive role against insects. from isotope-feeding studies, two modes of bcfa elongation have been proposed: (1) fatty acid synthase-mediated two-carbon elongation in the high acyl sugar-producing tomato species solanum pennellii and datura metel; and (2) alpha-keto acid elongation-mediated one-carbon increments in several tobacco (nicotiana) species an ... | 2008 | 18931142 |
| molecular and biological characterization of tomato chlorotic mottle virus suggests that recombination underlies the evolution and diversity of brazilian tomato begomoviruses. | abstract tomato chlorotic mottle virus (tocmov) is an emerging begomovirus species widely distributed throughout tomato-growing regions of brazil. tocmov appears to have expanded its geographic range recently, invading tomato-growing areas that were free of begomovirus infection before 2004. we have determined the first complete genome sequence of an infectious tocmov genome (isolate ba-se1), which is the first begomovirus species isolated in the northeast of brazil. when introduced by particle ... | 2007 | 18943601 |
| virus diseases in the tobacco fields of guilan and western azerbaijan provinces of iran. | tobacco (nicotiana tabacum l.) is one of the important industrial plants in iran. viruses as an important group of plant pathogens cause many losses on the quality and quantity of tobacco crop. there was few information on the types of plant viruses infecting the tobacco fields of guilan and almost no information for western azerbaijan province. during 2005-2007, leaf samples were taken from symptomatic plants in the growing areas of these two provinces. the observed symptoms on plants in the fi ... | 2008 | 19226768 |
| melatonin and serotonin in flowers and fruits of datura metel l. | datura metel is a plant that contains several different neurologically active phytochemicals which affect human health. on-going research has examined the potential role of the human neuroindoles, melatonin and serotonin, in medicinal plants with neurological efficacy. in this report, we describe the quantification of melatonin and serotonin in flowers and developing fruits of datura metel and the effects of cold stress on the levels of these neuroindoles in the reproductive tissues of this plan ... | 2009 | 19732299 |
| anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, haemonchus contortus. | the source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. the egg hatching and larvicidal effect of indigenous plant extracts were investigated against the sheep parasite, haemonchus contortus. the purpose of the present study was to assess the efficacy of leaf, bark, and seed ethy ... | 2010 | 21161270 |
| ethnobotanical survey of medicinal plants commonly used by kani tribals in tirunelveli hills of western ghats, india. | for thousands of years, medicinal plants have played an important role throughout the world in treating and preventing a variety of diseases. kani tribal people in tirunelveli hills still depend on medicinal plants and most of them have a general knowledge of medicinal plants which are used for first aid remedies, to treat cough, cold, fever, headache, poisonous bites and some simple ailments. | 2011 | 21291981 |
| control of charcoal rot fungus macrophomina phaseolina by extracts of datura metel. | methanolic leaf and fruit extracts of datura metel were found highly effective in suppressing against macrophomina phaseolina, the cause of charcoal rot disease. these extracts were further subjected to successive fractionation with n-hexane, chloroform, ethyl acetate and n-butanol. all the concentrations (3.125-200 mg ml(-1)) of chloroform, ethyl acetate and n-butanol fractions of leaf extract, and n-hexane fraction of fruit extract completely inhibited the target fungal growth. two compounds a ... | 2011 | 22004473 |
| cytotoxic activity of withanolides isolated from tunisian datura metel l. | withanolide-type steroids, withametelin q (1) and 12α-hydroxydaturametelin b (2) along with three known withanolides, were isolated from leaves of datura metel l. (solanaceae). the respective structures, characterized mainly by nmr spectroscopy, were identified as (20r,22r,24r)-21,24-epoxy-1α,3β-dihydroxywitha-5,25(27)-dienolide-3-o-β-d-glucopyranoside (1) and (20r,22r,24r)-12α,21,27-trihydroxy-1-oxowitha-2,5,24-trienolide-27-o-β-d-glucopyranoside (2). the cytotoxicity of isolated compounds was ... | 2011 | 21851957 |
| [scopolamine and hyoscyamine synthesis in hair roots culture of datura metel]. | to establish the hair roots culture system of datura metel and study the hair roots growth and biosynthesis of scopolamine and hyoscyamine in hair roots culturing system. | 2012 | 23397717 |
| evaluation of in vitro antiviral activity of datura metel linn. against rabies virus. | the soxhlet and cold extracts of datura metel linn. were evaluated for in vitro antirabies activity. | 0 | 27695266 |
| induction of resistance in tomato plants against tomato mosaic tobamovirus using beneficial microbial isolates. | the possibility of making use of the phenome non of systemic acquired resistance (sar) to control viruses achieved by the soaking treatment of tomato seeds cv. castl rock with three growth forms to bacillus circulans, pseudomonas fluorescens 2 and trichoderma harzianum against tomato mosaic tobamovirus (tomv) infection. all the application forms of beneficial biotic inducers were reduced the mean number of tomv local lesions on datura metel. p. fluorescens 2 was found to be the best treatment in ... | 2013 | 24494520 |
| datura metel-synthesized silver nanoparticles magnify predation of dragonfly nymphs against the malaria vector anopheles stephensi. | malaria is a life-threatening disease caused by parasites transmitted to people and animals through the bites of infected mosquitoes. the employ of synthetic insecticides to control anopheles populations leads to high operational costs, non-target effects, and induced resistance. recently, plant-borne compounds have been proposed for efficient and rapid extracellular synthesis of mosquitocidal nanoparticles. however, their impact against predators of mosquito larvae has been poorly studied. in t ... | 2015 | 26337272 |
| ethnomedicinal plant use value in the lakki marwat district of pakistan. | medicinal plants are regional treasures for the treatment of many ailments. the present research investigated and documented knowledge of indigenous commonly used medicinal plants, including traditional names, preparations and uses, in the lakki marwat district of pakistan. the information gathered was statistically analyzed using the icf method to establish baseline data for more comprehensive investigations of bioactive compounds of indigenous medicinal plants. | 2014 | 25448507 |
| isolation and characterization of some phytochemicals from indian traditional plants. | the present study was designed to evaluate relative contribution of different polyphenols (total phenolics, flavonoids, flavonols) and their antioxidants activities in aqueous extracts of different parts of some plants; argemone mexicana, datura metel, calotropis procera, thevetia peruviana, and cannabis sativa. the antioxidants (total phenolics, flavonoids, flavones) were determined by chemical methods. the antioxidant capacities of these extracts were evaluated by frap assay. the results demon ... | 2012 | 23304524 |
| micropropagation: a tool for the production of high quality plant-based medicines. | medicinal plants are the most important source of life saving drugs for the majority of the world's population. the biotechnological tools are important to select, multiply and conserve the critical genotypes of medicinal plants. plant tissue culture techniques offer an integrated approach for the production of standardized quality phytopharmaceutical through mass-production of consistent plant material for physiological characterization and analysis of active ingredients. micropropagation proto ... | 2006 | 16472132 |
| [eco-physiological investigations on wild and cultivated plants in the negev desert : iii. daily courses of net photosynthesis and transpiration at the end of the dry period]. | the daily course of net photosynthesis and transpiration was measured with temperature and humidity controlled cuvettes at the end of the dry summer season in the negev desert. species studied included: dominant species of the natural vegetation, cultivated plants in the run-off farm avdat and permanently irrigated plants. an analysis of the influence of single climatic factors on gas exchange was given in part ii of this publication. the reactions of the plants to complex changes in all the env ... | 1972 | 28313070 |
| [eco-physiological investigations on wild and cultivated plants in the negev desert : ii. the influence of climatic factors on carbon dioxide exchange and transpiration at the end of the dry period]. | the influence of climatic factors on net photosynthesis, dark respiration and transpiration was investigated in the negev desert at the end of the dry summer period when plant water stress was at a maximum. species studied included: dominant species of the natural vegetation (artemisia herba-alba, hammada scoparia, noaea mucronata, reaumuria negevensis, salsola inermis, zygophyllum dumosum), cultivated plants receiving rainfall and run-off water during the winter season in the run-off farm avdat ... | 1972 | 28311256 |
| evaluation of in vitro cytotoxic and antioxidant activity of datura metel linn. and cynodon dactylon linn. extracts. | to evaluate in vitro cytotoxicity and antioxidant activity of datura metel l. and cynodon dactylon l. extracts. | 2017 | 27034603 |
| steroidal saponins from datura metel. | datura metel l. (solanaceae) is an annual herb that has been widely used in the traditional medicine for the treatment of coughs, bronchial asthma, and rheumatism. chemical investigation of an acidic methanol extract of the whole plants of d. metel resulted in the isolation of five new steroidal saponins (1-3, 5, and 6), named metelosides a-e, and four known compounds (4, 7-9). their structures were elucidated by extensive spectroscopic methods, including 1d and 2d nmr and ms spectra. the struct ... | 2017 | 28237768 |
| investigation of anti-asthmatic potential of kanakasava in ovalbumin-induced bronchial asthma and airway inflammation in rats. | kanakasava is an indian traditional ayurvedic formulation containing datura (datura metel), vasaca (adhatoda vasica), dhataki (woodfordia fruticosa) and grape (vitis vinifera) extracts as major constituents and used to treat pulmonary diseases including coughing, breathing difficulty and asthma. the present study was designed to assess the safety and therapeutic efficacy of kanakasava against ovalbumin-induced bronchial asthma and related airway inflammation in rats due to lack of evidence based ... | 2017 | 27502540 |
| [study on chemical constituents in seeds of datura metel from xinjiang]. | to study the chemical constituents in the seeds of datura metel from xinjiang province. | 2015 | 26983236 |
| chemical composition and cytotoxicity of the essential oil from different parts of datura metel l. | the essential oil from different parts of datura metel l. were extracted using hydrodistillation and gc-ms was used to analyse the essential oil. the main components of flowers were ketone (23.61%) and ethyl palmitate (15.84%). the main components of leaves were ketone (18.84%) and phytol (18.71%). ketone (39.45%) and phytol (31.32%) were the major components of petioles. palmitic acid (30.60%) and ethyl linoleate (21.56%) were the major components of seeds. the major ingredient of roots was pal ... | 2016 | 26418519 |
| identification of potential plant extracts for anti-tick activity against acaricide resistant cattle ticks, rhipicephalus (boophilus) microplus (acari: ixodidae). | to develop an eco-friendly tick control method, seven plant extracts were prepared using 50 and 95% ethanol and evaluated for acaricidal activity against cattle tick, rhipicephalus (boophilus) microplus. the adult immersion test was adopted for testing different extracts. based on 72 h screening criterion, 95% ethanolic extracts of datura metel fruits and argemone mexicana whole plant were found effective showing more than 50% mortality of treated ticks. the 95% ethanolic extracts of d. metel fr ... | 2015 | 25717008 |
| [plant regeneration and clonal propagation system of datura metel]. | to study the condition of plant regeneration and clonal propagation system of datura metel. | 2014 | 25095331 |
| new anti-inflammatory withanolides from the leaves of datura metel l. | nine new withanolides, named daturafolisides a-i (1-9), along with six known compounds (22r)-27-hydroxy-7α-methoxy-1-oxowitha-3,5,24-trienolide-27-o-β-d-glucopyranoside, daturataturin a, daturametelin j, daturataurin b, baimantuoluoside b, 12-deoxywithastramonolide (10-15), were isolated from the leaves of datura metel l. the structures and absolute stereochemistry of these compounds were elucidated by means of spectroscopic methods including 1d and 2d nmr techniques, mass spectrometry and circu ... | 2014 | 24844203 |
| five withanolides from the leaves of datura metel l. and their inhibitory effects on nitric oxide production. | four new withanolides named dmetelins a-d (compounds 1-4), along with the known compound 7α,27-dihydroxy-1-oxo-witha-2,5,24-trienolide (5) were isolated from the leaves of datura metel l. (solanaceae). their structures were elucidated on the basis of detailed analysis of 1d and 2d nmr and mass spectrometry data. all the compounds were evaluated for their inhibitory effects on lipopolysaccharide (lps)-induced nitric oxide (no) production in raw264.7 cells. compounds 1, 4 and 5 showed significant ... | 2014 | 24731984 |
| retarded hippocampal development following prenatal exposure to ethanolic leaves extract of datura metel in wistar rats. | datura metel contains atropine alkaloids and has been used to treat complication like asthma and, bronchitis, because of its anticholinergic properties. | 2013 | 24665157 |
| two novel norwithasteroids with unusual six- and seven-membered ether rings in side chain from flos daturae. | chemical investigation of 50% ethanol eluate fraction of macroporous resin for the flower of datura metel l. collected in jiangsu province of china resulted in the isolation of two novel naturally occurring norwithasteroids, baimantuoluoline i (1) and baimantuoluoside j (2). their structures were elucidated as 5 α , 6 β , 12 β -trihydroxy-1-oxo-2-en-ergosta-21,24;22,29-diepoxy-26-carboxylic acid (1) and 5 α , 6 β , 12 β , 25-tetrahydroxy-1-oxo-2-en-ergosta-21,24;22,29-diepoxy-26-carboxylic acid ... | 2013 | 23606878 |
| [identification of daturae flos and its adulterants based on dna barcoding technique]. | to identify the original plant of daturae flos from its adulterants by dna barcoding, the sequences of its2, psba-trnh, matk, rbcl of four species including datura metel, darura innoxia, darura stramonium and brugmansia arborea were compared and analyzed. the pcr and sequencing success rate of the four regions (its2, psba-trnh, matk, rbcl) was 100%, 90%, 100% and 85%, respectively. sequences were assembled with codoncode aligner. k2p distances were calculated and nj tree was performed by mega 4. ... | 2011 | 22260038 |
| two new withanolide lactones from flos daturae. | chemical investigation of the 50% ethanol eluate fraction from a macroporous resin of flowers of datura metel l. collected in the jiangsu province of china resulted in the isolation of two new withanolides, baimantuoluoline g (1) and baimantuoluoside h (2). their structures were elucidated as (12β,6β,22r)-1,10-seco-6,12,27-trihydroxy-26-oxo-witha-3,5,24-trienolide-1-oic acid-ε-lactone (1) and (5β,6α,12β,22r)-5,6,12,27-tetra-hydroxy-1,26-dioxo-witha-2,24-dienolide-27-o-β-glucopyranoside (2) on th ... | 2011 | 21747337 |
| biochemical and structural characterization of recombinant hyoscyamine 6β-hydroxylase from datura metel l. | hyoscyamine 6β-hydroxylase (h6h; ec 1.14.11.11), an important enzyme in the biosynthesis of tropane alkaloids, catalyzes the hydroxylation of hyoscyamine to give 6β-hydroxyhyoscyamine and its epoxidation in the biosynthetic pathway leading to scopolamine. datura metel produces scopolamine as the predominant tropane alkaloid. the cdna encoding h6h from d. metel (dmh6h) was cloned, heterologously expressed and biochemically characterized. the purified recombinant his-tagged h6h from d. metel (dmrh ... | 2010 | 20951598 |
| herbicidal effects of extracts and residue incorporation of datura metel against parthenium weed. | the present study was designed to evaluate the herbicidal activity of datura metel against the noxious weed parthenium (parthenium hysterophorus l.). in a laboratory bioassay, the effect of aqueous, methanol and n-hexane shoot and root extracts of 5, 10, 15 and 20% w/v (on a fresh weight basis) concentration of d. metel were tested against the germination and seedling growth of parthenium. both aqueous and methanol extracts markedly suppressed the germination and seedling growth of parthenium. g ... | 2010 | 20812130 |
| baimantuoluosides d-g, four new withanolide glucosides from the flower of datura metel l. | in our search for bioactive anti-psoriasis compounds from the flower of datura metel l, we isolated four new withanolide glucosides, baimantuoluosides d, e, f and g (1-4). the structures of the new compounds are (5alpha,6alpha,7beta,22r)-5,6,7,27-tetrahydroxy-1-oxowitha-2,24-dien-27-o-beta-d-glucopyranoside (1), (5alpha,6beta,7alpha,22r)-5,6,7,27-tetrahydroxy-1-oxowitha-2,24-dien-27-o-beta-d-glucopyranoside (2), (5alpha,6beta,7alpha,12beta,22r)-5,6,7,12,27-pentahydroxy-1-oxowitha-2,24-dien-27-o- ... | 2010 | 20803115 |
| two new amide alkaloids from the flower of datura metel l. | two new amide alkaloids were isolated from the alkaloidal fraction of datura metel l. their structures were elucidated as (e)-methyl 4-(3-(4-hydroxyphenyl)-n-methylacrylamido) butanoate and 6,7-dimethyl-1-d-ribityl-quinoxaline-2,3(1h,4h)-dione-5'-o-β-d-glucopyranoside on the basis of spectroscopic methods including hresims, 1d and 2d nmr. tropane alkaloids have for a long time been considered as characteristic ingredients of d. metel. however, the presence of amide alkaloids in d. metel was repo ... | 2010 | 20600686 |
| [clinical analysis of poisoning induced by intake of datura metel]. | | 2009 | 19493501 |
| authentication of the 31 species of toxic and potent chinese materia medica by light microscopy, part 3: two species of t/pcmm from flowers and their common adulterants. | toxic and potent chinese materia medica (t/pcmm) has become a hot and sensitive topic as more and more people around the world are interested in the safety of herbal medicines. t/pcmm is irreplaceable in treating some diseases; but it can easily cause serious problems if confused with other herbal medicines. accurate identification is essential to ensure their safe use, but up to now, the literature on the authentication of t/pcmm is scant. thus, we are undertaking a study of 31 t/pcmm originati ... | 2009 | 19208392 |
| [a case of food poisoning due to ingestion of eggplant, solanum melongena, grafted on devil's trumpet, datura metel]. | a husband and wife successively visited an emergency room with symptoms of staggering, slurred speech, mydriasis, and drowsiness, three hours after separately eating spaghetti with meat sauce. the sauce contained eggplant that had been grafted onto a devil's trumpet, datura metel. scopolamine and atropine were detected in the leftover sauce and in the sera of the patients. this is the first case of food poisoning related to datura in okinawa, japan and also might be the first report of food pois ... | 2008 | 19029791 |
| chemical constituents from the flower of datura metel l. | phytochemical investigation of the 50% ethanol eluate fraction of macroporous resin for the flower of datura metel l. led to the isolation of a new compound named yangjinhualine a (1) and five known megastigmane sesquiterpenes through repeated silica gel and ods column chromatography and semipreparative hplc. the structures of these compounds were determined by spectroscopic methods, including 2d-nmr, esi-ms, and hr-esi-ms spectrometry as well as by comparison with published data. five known meg ... | 2008 | 18806950 |
| two instances of chinese herbal medicine poisoning in singapore. | datura metel l. (yangjinghua) is a toxic herb that contains anticholinergic compounds. inappropriate consumption of this herb could result in anticholinergic poisoning. clinical features of such poisoning have not been previously described. we report two such cases. both patients had taken brews of datura metel l., and developed poisoning soon afterwards. prominent clinical features included confusion, dilated pupils, absence of sweating, and the absence of sluggish bowel sounds. no flushing of ... | 2008 | 18465037 |
| molecular cloning and characterization of a new cdna encoding hyoscyamine 6beta-hydroxylase from roots of anisodus acutangulus. | a new full-length cdna encoding hyoscyamine 6beta-hydroxylase (designated as aah6h, genbank accession no. ef187826), which catalyzes the last committed step in the scopolamine biosynthetic pathway, was isolated from young roots of anisodus acutangulus by rapid amplification of cdna ends (race) for the first time. the full-length cdna of aah6h was 1380 bp and contained a 1035 bp open reading frame (orf) encoding a deduced protein of 344 amino acid residues. the deduced protein had an isoelectric ... | 2007 | 17927905 |
| cytotoxic withanolides from the flowers of datura metel. | chemical investigation of a methanol extract of the flowers of datura metel has led to the isolation of 10 new withanolides, withametelins i-p (1-8), 1,10-seco-withametelin b (9), and 12beta-hydroxy-1,10-seco-withametelin b (10), together with seven known withanolides. the structures of 1-10 were elucidated by means of spectroscopic methods, and the absolute stereochemistry of 1 was confirmed by single-crystal x-ray analysis. compounds 1, 3, 4, and 6 exhibited cytotoxic activities against a549 ( ... | 2007 | 17583953 |
| comparative study of responses in four datura species to a zinc stress. | the effects of zinc toxicity on the growth and the photosynthetic activities of four datura species (datura metel, datura innoxia, datura sanguinea, datura tatula) were studied using various znso4 concentrations (0, 1, 2.5 and 5 mm) added in the coic lessaint solution. growth, photosynthesis, chlorophyll fluorescence and chlorophyll concentration were measured after 20 days of zinc stress. these parameters were severely reduced by this heavy metal. the zinc excess involves the stomate closing, t ... | 2005 | 15823334 |
| evaluation of analgesic effect of datura fastuosa leaves and seed extracts. | the aqueous extracts of datura fastuosa leaves and seeds were evaluated for the analgesic effect on acetic acid-induced writhing and hot plate reaction in mice. according to the results, d. fastuosa leaves and seeds extracts at oral doses of 400 and 800 mg/kg are effective as analgesic. the analgesic activity of leaf extract is reduced by naloxone but not that of seed extract. | 2003 | 12837368 |
| investigation of the effect of space environment on the contents of atropine and scopolamine in datura metel by capillary zone electrophoresis. | the seeds of datura metel were carried aboard a retrievable satellite and exposed to space environment. the effects of space environment (weightlessness and ionizing radiation) on the contents of atropine and scopolamine in d. metel were investigated by using an effective capillary zone electrophoresis (cze) method, which employed 50 mmol/l phosphate buffer (ph 8) containing 10% (v/v) tetrahydrofuran as the running buffer. the results showed that the contents of atropine and scopolamine varied t ... | 2003 | 12684101 |
| steroidal compounds from in vitro regenerated shoots of datura metel. | regeneration of adventitious shoots from young leaves of datura metel is described. shoot buds developed on ms medium with 2 mg/l and elongated on hormone-free solid basal medium. the microshoots failed to produce alkaloids, but a number of steroidal compounds were detected. the c(28) sterol 3beta,24xi-dihydroxy-ergosta-5,25-dienolide, regarded as the precursor of withanolides and related steroidal lactones, was identified. the withanolide 12-deoxywithastramonolide was also detected. | 2003 | 12628388 |
| calystegine distribution in some solanaceous species. | the distribution of eight calystegines (a(3), a(5), b(1), b(2), b(3), b(4), c(1) and n(1)) and their content was investigated by gas chromatography coupled to mass spectrometry (gc-ms) in datura metel, atropa belladonna, hyoscyamus albus, mandragora autumnalis, solanum sodomaeum, withania somnifera, withania frutescens and brunfelsia nitida. the most frequently encountered calystegines were a(3), b(1), b(2) and b(3), while distribution of n(1) and c(1) was more limited. in all the investigated s ... | 2001 | 11557078 |
| [acute intoxication with "sobi-lobi" (datura). four cases in niger]. | the datura (datura stramonium, datura metel) belongs to a group of plants from the solanacies variety and can be the cause of voluntary intoxication. in nigher, its flowers and seeds are used for their hallucinogenic properties. its alcaloïds have an atropine-like effect. we are reporting here the first four cases of voluntary intoxication among teenagers in niamey (republic of niger). the symptoms, which happened to be identical for the four patients, lead us quickly towards the possibility of ... | 2000 | 11204734 |
| [chirality of natural products: hyoscyamine and scopolamine]. | monthly changes of the content-ratio between s-(-)- and r-(+)-hyoscyamine as well as those between s-(-)- and r-(+)-scopolamine in the leaves of datura metel l. cultivated in the field, were quantitatively analyzed by the use of hplc with a chiral adsorbent. it was found that s-(-)-isomer was predominant for hyoscyamine and the ratio of r-(+)-isomer gradually increased during the growth, whereas in the case of scopolamine, s-(-)-isomer was the sole one found throughout the cultivation period. th ... | 2000 | 11082712 |
| genotypes and alkaloid contents of datura metel varieties. | datura metel l. var. muricata (bernh.) danert was found to be double recessive with respect to the genes concerning the color and form of the corolla by breeding experiments involving four varieties, i.e. var. metel (white, simple corolla), var. rubra (purple, simple), var. fastuosa (purple, double or triple) and var. muricata (white, purple). the results support the proposal by danert and others that these variants should be considered as varieties or forms of a single species, datura metel. th ... | 1996 | 8874823 |
| variation of scopolamine and atropine in different parts of datura metel during development. | scopolamine and atropine contents in the whole plant of datura metel l. increased gradually with the progress of developmental growth, and were most pronounced when the plant was at the end of its reproductive stage. the highest percentage of scopolamine accumulation in the root was after 16 weeks. the root was the organ which often accumulated higher amounts of atropine. the aerial parts, if compared with the root of the plant, usually accumulated relatively higher amounts of scopolamine and re ... | 1995 | 17238092 |
| herbal poisoning caused by adulterants or erroneous substitutes. | six cases of herbal poisoning involving six patients in hong kong, taipei and kuala lumpur are reported. the sources of poisoning were identified as adulterants (podophyllum emodi) or erroneous substitutes (datura metel). in cases of suspected herbal poisoning, it is recommended that the prescriptions, herbal residues and herb samples should be collected for pharmacognostical and chemical analysis to substantiate the cause of poisoning. insofar as it is possible, an estimate of the amount of her ... | 1994 | 7966541 |
| [optinum for extracting total alkaloids in datura metel l]. | | 1994 | 7980860 |
| identical amino acid sequence of ferredoxin from datura metel and d. innoxia. | the complete amino acid sequence of the respective [2fe-2s] ferredoxins from datura metel (d. alba) and d. innoxia, have been determined by automated edman degradation of the entire cm-protein and of the peptides obtained by tryptic digestion and cnbr treatment. the ferredoxins from the two plants had an identical amino acid sequence, suggesting a very close taxonomic relationship between the two. this is the first report for the identical primary structure of fds from different species of highe ... | 1994 | 7764481 |
| variation of total hyoscine content of cultivated datura metel linn. | a titrimetric method is described for rapid assay of hyoscine content of organs of locally cultivated datura metel linn. raw materials required (approximately 1.5 gm) are much less than those required for conventional percolation processes. using the procedure, the flowers have been shown to be richest in hyoscine, only minimal levels of alkaloids being detected in the fruits. furthermore, the total alkaloid content of d. metel has been shown to peak in the hot dry season, and at its lowest duri ... | 1993 | 7839880 |
| biological studies of indigenous medicinal plants--i: physicochemical and antimicrobial screening of non-alkaloidal constituents of some solanaceous seeds. | the seeds of atropa belladonna datura fastuosa d. stramoniurn and hyoscyamus niger were investigated for the isolation and anti-microbial activity of non-alkaloidal constituents. the compounds were separated by column chromatography and spectral studies i.e. ir and uv were performed to ascertain their chemical nature. the anti-microbial activity of crude extracts and purified compounds was tested against gram positive and gram negative bacteria and a fungus. all the micro-organisms tested were m ... | 1992 | 16414703 |
| a new withanolide, datumetelin, from the leaves of datura metel. | | 1988 | 17265324 |
| [acute poisoning with atropine-like alkaloids of datura metel]. | | 1988 | 2973941 |
| [poisoning caused by datura fastuosa (ketjubung)]. | | 1985 | 4033823 |
| the tonoplast, a specific marker of embryogenic microspores of datura cultured in vitro. | microspores of datura metel l. have a thin tonoplast which, upon culture in vitro, develops a more or less uniform coating or deposit (as early as 12 h). this response was specific for embryogenic microspores, thereby making it possible to distinguish them from the nonembryogenic microspores. cytochemical tests indicated the presence of tannins in this deposit which persists only until the early globular stage of embryogenesis. thus tannin-coated tonoplasts can be used as a reliable and specific ... | 1983 | 6618913 |
| change in the amino-acid content during male gametophyte formation of datura metel in situ. | determination of the free and bound aminoacids during microsporogenesis of datura metel showed that the principal amino-acids were proline, glutamic acid, aspartic acid, threonine-serine and alanine. of these, only proline showed a consistent increment during pollen development. in contrast, aspartic acid and lysine decreased in the later stages of microsporogenesis. the amounts of other amino-acids did not show any consistent pattern. four amino-acids, namely proline, glutamic acid and threonin ... | 1978 | 24317576 |
| letter: poisoning due the datura fastuosa. | | 1976 | 957466 |
| poisoning due to ingestion of the seeds of kechubong (datura fastuosa) for its ganja-like effect in singapore. | | 1974 | 4835325 |
| the distribution of total alkaloids and major components in the different organs of datura metel var. fastuosa at various stages of growth. | | 1973 | 4732118 |
| the early ontogeny of embryoids and callus from pollen and subsequent organogenesis in anther cultures of datura metel and rice. | haploidy induction through anther culture has been examined in datura metel and rice with a view to tracing the precise sequence of development of the pollen, either directly or through an intervening callus, into an embryo and seedling. in d. metel, the vegetative cell of the young pollen grain assumes the major role in formation of embryos whereas the generative cell and its few derivatives degenerate. embryos and seedlings arising directly from pollen without an intervening callus phase alway ... | 1972 | 24481698 |
| [on the biogenesis of datura alkaloids; study of ontogenic variations by use of the chromatographic method in datura metel sims]. | | 1965 | 14332509 |
| hemagglutinins in extracts of israeli plants. ii. the chemical nature of the active substance in datura metel. | | 1959 | 13631238 |
| [pharmacognostic study of datura metel l. & datura innoxia mill]. | | 1957 | 13494175 |
| [structure of leaves of datura innoxia mill. (erroneously classified as datura metel l.)]. | | 1955 | 13248654 |
| [content of alkaloids in datura metel l. during its vegetation]. | | 1952 | 13033005 |
| effect of pruning and exploration on the growth and alkaloidal content of datura metel linn. | | 1948 | 18880180 |
| effect of colchicine treatment on the alkaloidal content of datura metel. | | 1948 | 18915114 |