Publications

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single nucleotide primer extension (snupe) analysis of the g6pd gene in somatic cells and oocytes of a kangaroo (macropus robustus).cdna sequence analysis of the x-linked glucose-6-phosphate dehydrogenase (g6pd) gene has shown a base difference between two subspecies of the kangaroo, macropus robustus robustus (wallaroo) and m. r. erubescens (euro). a thymine residue in the wallaroo at position 358 in exon 5 has been replaced by a cytosine residue in the euro, which accounts for the previously reported electrophoretic difference between the two subspecies. this base difference allowed use of the single nucleotide primer exte ...200010893863
analysis of dnase 1 sensitivity and methylation of active and inactive x chromosomes of kangaroos (macropus robustus) by in situ nick translation.the overall nuclease sensitivity and methylation of active and inactive x chromosomes of kangaroos were examined by in situ nick translation. cultured fibroblasts of subspecies wallaroo-euro (macropus robustus robustus; macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived x chromosomes to be distinguished. no difference was found between the active and inactive x chromosomes with dnase i or mspi digestion. when chromosomes were digested with the methyla ...19938381740
analyses of mitochondrial amino acid sequence datasets support the proposal that specimens of hypodontus macropi from three species of macropodid hosts represent distinct species.hypodontus macropi is a common intestinal nematode of a range of kangaroos and wallabies (macropodid marsupials). based on previous multilocus enzyme electrophoresis (mee) and nuclear ribosomal dna sequence data sets, h. macropi has been proposed to be complex of species. to test this proposal using independent molecular data, we sequenced the whole mitochondrial (mt) genomes of individuals of h. macropi from three different species of hosts (macropus robustus robustus, thylogale billardierii an ...201324261823
a highly repeated dna from the genome of the wallaroo (macropus robustus robustus).the major satellite of m. r. robustus dna has been isolated in a ag+-cs2so4 gradient. it has a density of 1.710 g/cm(3) compared with 1.697 g/cm(3) for the bulk of the dna, and accounts for about 10% of the total dna. sequence heterogeneity within the satellite was shown by an increase in density to 1.715 g/cm(3) and by a reduction of 12 degrees c in the temperature of denaturation (tm) after renaturation. the satellite was found to occur in the centromeric regions of all chromosomes. this patte ...19816167251
protein turnover in two kangaroo sub-species (macropus robustus robustus and m. r. erubescens) from divergent habitats and the sympatric feral goat (capra hircus).1. whole-body protein turnover was measured by a single-injection [15n]glycine method in the euro (macropus robustus erubescens), wallaroo (m. r. robustus) and goat (capra hircus). 2. dietary nitrogen (n) intake was greater (p < 0.05) in the goat (1.17 g n/kg 0.75 x day) than in the kangaroo sub-species and greater (p < 0.05) in the wallaroo (1.07 g n/kg 0.75 x day) than in the euro (0.93 g n/kg 0.75 x day). similar (0.29-0.34 g n/kg 0.75 x day) among the species. 3. crude protein (cp) turnover ...19938101778
differences in a ribosomal dna sequence of morphologically indistinguishable species within the hypodontus macropi complex (nematoda: strongyloidea).the nucleotide sequence of the second internal transcribed spacer (its-2) from ribosomal dna has been determined for 3 members of the hypodontus macropi species complex. sequences were compared from nematodes collected from 3 species of australian macropodid marsupial, petrogale persephone, macropus robustus robustus and thylogale billardierii. the its-2 of each operational taxonomic unit ranged from 287 to 292 bases in length, and had a gc content of 36.6-40.1%. differences in nucleotide sequen ...19957635644
studies on metatherian sex chromosomes. i. inheritance and inactivation of sex-linked allelic genes determining glucose-6-phosphate dehydrogenase variation in kangaroos.wallaroos (macropus robustus robustus), which have the g6pd-f electrophoretic phenotype, crossed with euros (m.r.erubescens), of g6pd-s phenotype, produced f1 animals which had only the maternal g6pd type regardless of the direction of the cross. when f1 hybrids were backcrossed to wallaroos or euros, backcross progeny of either perental phenotype resulted. sex-linked inheritance of allelic g6pd genes is shown to occur in wallaroos, euros and red kangaroos (m. rufus). dose compensation for x chr ...19751225290
molecular evidence for a cryptic species within the parasitic nematode macroponema comani (strongyloidea: cloacininae).nematodes resembling macroponema comani, a common parasite of eastern grey kangaroos, macropus giganteus, in eastern australia were collected from an unexpected host species, the northern wallaroo, macropus robustus woodwardi, in the northern territory, representing a highly disjunct occurrence. although these specimens showed no morphological differences when compared with ma. comani from m. giganteus, sequencing of the first and second internal transcribed spacers its-1 and its-2 of the nuclea ...201222710283
single-strand conformation polymorphism analysis of genetic variation in labiostrongylus longispicularis from kangaroos.single-strand conformation polymorphism (sscp) analysis was employed to screen for sequence heterogeneity in the second internal transcribed spacer (its-2) of ribosomal (r) dna of labiostrongylus longispicularis, a parasitic strongylid nematode occuring in some species of kangaroo in different geographical regions of australia. the results showed that most of the nematodes screened had different sscp profiles, which were subsequently shown to correspond to polymorphisms and/or an indel in the it ...200111465490
bacteriophages from the forestomachs of australian marsupials.bacteriophages were observed in forestomach contents from three species of australian macropodoid marsupials possessing a foregut fermentative digestion: the eastern grey kangaroo (macropus giganteus), the eastern wallaroo (macropus robustus robustus), and the rufous bettong (aepyprymnus rufescens). forty-six morphologically distinct phage types, representing the families myoviridae, siphoviridae, and podoviridae, were identified. the range of forms varied between host species. the greatest dive ...19911785938
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