| coaggregation studies of the eubacterium species. | eubacterium species are gram-positive anaerobic rods that are frequently isolated from subgingival plaque of periodontal pockets. five eubacterium species were tested for their ability to coaggregate with 33 oral bacterial strains. using visual and turbidimetric assays, coaggregation was observed among eubacterium brachy, eubacterium nodatum, eubacterium alactolyticum and eubacterium limosum strains only when tested with fusobacterium nucleatum strains; eubacterium saburreum displayed only weak ... | 1992 | 1494452 |
| eubacterium nodatum mimics actinomyces in intrauterine device-associated infections and other settings within the female genital tract. | eubacterium nodatum is an obligately anaerobic, gram-positive, branching rod that markedly resembles actinomyces, particularly actinomyces israelii, in its cellular and colonial characteristics. its isolation from the female genital tract was examined for a study period in which use of intrauterine contraceptive devices (iuds) was common, and additional characteristics of the organism were investigated. fifteen genital isolates of e nodatum were all associated with the presence of a foreign body ... | 1992 | 1553171 |
| growth stimulation of treponema denticola by periodontal microorganisms. | previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ... | 1990 | 2321929 |
| chemical composition of eubacterium nodatum cell wall peptidoglycan. | the structure of eubacterium nodatum cell wall peptidoglycan was investigated. the peptide subunit of e. nodatum peptidoglycan has the following structure: l-ala-d-glu (gly)-l-orn-d-ala. the carboxyl group of alanine occupying position 4 is attached to the delta-amino group of ornithine of an other subunit by the cross-linking bridge l-ala-l-ala-l-orn. all glycine molecules are connected with the alpha-carboxyl group of glutamic acid with the ratio being 0.5-1. the hydrolysis of e. nodatum pepti ... | 1989 | 2742451 |
| characteristics and sites of infection of eubacterium nodatum, eubacterium timidum, eubacterium brachy, and other asaccharolytic eubacteria. | three new species, eubacterium nodatum, eubacterium timidum, and eubacterium brachy, were described, primarily from subgingival samples taken from patients with moderate and severe adult periodontitis. except for the isolation of e. brachy from a pleuropulmonary infection, these species have not been reported from other infected body sites. we report on the isolation of these species and an undescribed group (d-6) of asaccharolytic eubacteria also found in periodontal disease from numerous diffe ... | 1987 | 3624445 |
| serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis. | in the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ... | 1985 | 3988337 |
| the gingival immune response to periodontal pathogens in juvenile periodontitis. | a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ... | 1994 | 7870467 |
| phylogeny of oral asaccharolytic eubacterium species determined by 16s ribosomal dna sequence comparison and proposal of eubacterium infirmum sp. nov. and eubacterium tardum sp. nov. | 16s rrna gene sequences of eubacterium brachy, eubacterium nodatum, eubacterium saphenum, eubacterium timidum, and two previously unnamed taxa were determined. the results of a phylogenetic analysis indicated that all of the strains sequenced belonged to a deep branch of the low-g+c-content gram-positive group. the levels of 16s ribosomal dna sequence similarity between species were low, suggesting that a number of genera may be represented in this group. the representatives of the two unnamed t ... | 1996 | 8863423 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| restriction fragment-length polymorphism analysis of 16s rdna from oral asaccharolytic eubacterium species amplified by polymerase chain reaction. | restriction fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic eubacterium species, that is, eubacterium brachy, eubacterium exiguum, eubacterium lentum, eubacterium minutum, eubacterium nodatum, eubacterium saphenum, eubacterium timidum and 33 asaccharolytic eubacterium strains isolated from oral sites. the 16s rrna gene sequences from isolated genomic dna samples we ... | 1998 | 9573818 |
| diversity of oral asaccharolytic eubacterium species in periodontitis--identification of novel phylotypes representing uncultivated taxa. | oral asaccharolytic eubacterium species are associated with periodontal disease and other oral infections. the aim of this study was to use a culture-independent molecular technique to determine the diversity of asaccharolytic eubacterium species in subgingival plaque in periodontitis. an oligonucleotide pcr primer designed to amplify 16s rrna genes of the oral asaccharolytic eubacterium branch of the phylogenetic tree was constructed. this primer was used together with a universal primer in pcr ... | 1999 | 10204481 |
| cryptobacterium curtum gen. nov., sp. nov., a new genus of gram-positive anaerobic rod isolated from human oral cavities. | novel eubacterium-like isolates, strains 12-3t and kv43-b, which were isolated from the periodontal pocket of an adult patient with periodontal disease and necrotic dental pulp, respectively, were studied taxonomically and phylogenetically. the morphological and differential biochemical characteristics of these organisms are also described in this paper. these organisms were gram-positive, anaerobic, non-spore-forming, rod-shaped bacteria that were inert in most of the conventional biochemical t ... | 1999 | 10425779 |
| description of mogibacterium pumilum gen. nov., sp. nov. and mogibacterium vescum gen. nov., sp. nov., and reclassification of eubacterium timidum (holdeman et al. 1980) as mogibacterium timidum gen. nov., comb. nov. | a new genus, mogibacterium, is proposed for anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria which have been isolated from the periodontal pockets of adult human patients with periodontal disease and infected root canals. the novel isolates, strains d2-18t, ba11a-f and d5-2t, were inert in most of the conventional biochemical tests and phenotypically resemble asaccharolytic eubacterium species. the protein profiles of whole cells on sds-page gels and western immunoblotting reacti ... | 2000 | 10758875 |
| "checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study. | we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status. | 2000 | 10914791 |
| microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients. | polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities. | 2000 | 11073323 |
| checkerboard assessments of serum antibodies to oral microbiota as surrogate markers of clinical periodontal status. | this study addresses whether checkerboard assessments of serum igg antibodies to oral bacteria may serve as surrogate markers of clinical periodontal status in epidemiologic studies. | 2001 | 11142661 |
| change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole. | the current investigation evaluated changes in levels and proportions of 40 bacterial species in subgingival plaque samples during, immediately after and up to 1 year after metronidazole or amoxicillin therapy combined with srp. | 2001 | 11422580 |
| taxonomic characterization of mogibacterium diversum sp. nov. and mogibacterium neglectum sp. nov., isolated from human oral cavities. | novel isolates, strains hm-7, hm-6, hh-31, p9a-ht and ujb13-d, which were isolated from tongue plaque and necrotic dental pulp, were studied taxonomically and phylogenetically. these organisms were anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria that were inert in most of the conventional biochemical tests and phenotypically resemble mogibacterium species or asaccharolytic eubacterium species. the g+c contents of the dnas from the novel isolates ranged from 41 to 42 mol %. dna-d ... | 2002 | 11837293 |
| microbial profile on metallic and ceramic bracket materials. | the placement of orthodontic appliances creates a favorable environment for the accumulation of a microbiota and food residues, which, in time, may cause caries or exacerbate any pre-existing periodontal disease. the purpose of the present study was to compare the total bacterial counts present on metallic and ceramic orthodontic brackets in order to clarify which bracket type has a higher plaque retaining capacity and to determine the levels of streptococcus mutans and lactobacillus spp on both ... | 2002 | 12169034 |
| gram-positive anaerobic bacilli in human periodontal disease. | the uncertain taxonomy of oral anaerobic gram-positive bacilli and their generally slow growing nature has limited the understanding of their role in periodontal disease. the current objective was to design and use species-specific oligonucleotide probes to investigate the relationship of selected gram-positive anaerobic bacilli to periodontal disease. | 2004 | 15206913 |
| clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis. | the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy. | 2004 | 15367191 |
| microarrays complement culture methods for identification of bacteria in endodontic infections. | the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ... | 2005 | 15943771 |
| microbiological composition associated with interleukin-1 gene polymorphism in subjects undergoing supportive periodontal therapy. | interleukin-1 gene polymorphism (il-1 gene) has been associated with periodontitis. the present study examined the subgingival microbiota by il-1 gene status in subjects undergoing supportive periodontal therapy (spt). | 2006 | 16881809 |
| association of eubacterium nodatum and treponema denticola with human periodontitis lesions. | the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia. | 2006 | 16922925 |
| periodontal infection profiles in type 1 diabetes. | we investigated the levels of subgingival plaque bacteria and serum igg responses in patients with type 1 diabetes and non-diabetic controls of comparable periodontal status. | 2006 | 17092237 |
| clinical and microbiological profiles of human immunodeficiency virus (hiv)-seropositive brazilians undergoing highly active antiretroviral therapy and hiv-seronegative brazilians with chronic periodontitis. | this study compares the periodontal clinical profile and the composition of the subgingival microbiota of human immunodeficiency virus (hiv)-seropositive and hiv-seronegative subjects with chronic periodontitis. | 2007 | 17199544 |
| salivary microbiota of hiv-positive children and its correlation with hiv status, oral diseases, and total secretory iga. | this study aimed to investigate the prevalence and levels of salivary microorganisms in hiv-positive children, and their correlation to hiv status, oral lesions, and salivary iga levels. | 2008 | 18384349 |
| [microbiological and biochemical characteristics of inflammatory tissues in the periodontium]. | to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva. | 2008 | 18413987 |
| antimicrobial effectiveness of an herbal mouthrinse compared with an essential oil and a chlorhexidine mouthrinse. | the authors investigated mouthrinses' antimicrobial effectiveness against predominant oral bacteria, as determined by the minimum inhibitory concentration (mic). specifically, they evaluated an herbal mouthrinse, an essential oil rinse and a 0.12 percent chlorhexidine gluconate rinse. | 2008 | 18451378 |
| photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. | recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ... | 2009 | 18465191 |
| photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. | recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment. | 2008 | 18771363 |
| lysozyme and microbiota in relation to gingivitis and periodontitis. | the aim of this study was to investigate associations between the immune component of the oral cavity, lysozyme, in gingival crevicular fluid and unstimulated saliva, and colonization dynamics of periodontopathogenic bacteria from supra- and subgingival plaque samples in patients with gingivitis or periodontitis. | 2009 | 19179970 |
| serum antibodies to periodontal bacteria as diagnostic markers of periodontitis. | assessment of periodontal conditions in epidemiologic studies usually requires a clinical examination, which is resource-intensive. we investigated the ability of serum immunoglobulin g (igg) antibodies to periodontal bacteria to reflect clinical periodontal status. | 2009 | 19335084 |
| comparison between polymerase chain reaction-based and checkerboard dna hybridization techniques for microbial assessment of subgingival plaque samples. | to compare polymerase chain reaction (pcr) with subsequent reverse hybridization (micro-ident test) and checkerboard dna-dna hybridization for the identification of 13 bacterial species in subgingival plaque samples. | 2009 | 19563330 |
| microbiological profile of untreated subjects with localized aggressive periodontitis. | the microbial profile of localized aggressive periodontitis (lagp) has not yet been determined. therefore, the aim of this study was to evaluate the subgingival microbial composition of lagp. | 2009 | 19637996 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method. | to culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ... | 2010 | 20219690 |
| investigation of six selected bacterial species in endo-periodontal lesions. | didilescu ac, rusu d, anghel a, nica l, iliescu a, greabu m, bancescu g, stratul si. investigation of six selected bacterial species in endo-periodontal lesions. international endodontic journal, 45, 282-293, 2012. abstract: aim  to investigate and determine possible associations of six tested bacteria belonging to 'orange' and 'green' complexes, in endo-periodontal lesions: parvimonas micra, fusobacterium nucleatum, campylobacter rectus, eubacterium nodatum, eikenella corrodens and capnocytopha ... | 2011 | 22077868 |
| molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard dna-dna hybridization. | knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. the purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 dna probes for different bacterial species by using the checkerboard dna-dna hybridization (cddh) technique. | 2012 | 22196182 |
| necrotizing periodontal diseases in hiv-infected brazilian patients: a clinical and microbiologic descriptive study. | objective: nowadays, necrotizing periodontal diseases have a low prevalence; however, a better understanding of the etiopathogenesis of these diseases is necessary for determining more adequate preventive and therapeutic strategies. method and materials: from a pool of 1,232 hiv-infected patients, 15 presented with necrotizing periodontal diseases, which were evaluated by full-mouth periodontal clinical measurements. subgingival biofilm samples were collected from necrotizing lesions of six of t ... | 2012 | 22259811 |
| effect of periodontal therapy on the subgingival microbiota over a 2-year monitoring period. i. overall effect and kinetics of change. | to examine the 2-year post-therapy kinetics of change in the composition of subgingival biofilms. | 2013 | 23710672 |
| serum igg antibody levels to periodontal microbiota are associated with incident alzheimer disease. | periodontitis and alzheimer disease (ad) are associated with systemic inflammation. this research studied serum igg to periodontal microbiota as possible predictors of incident ad. | 2014 | 25522313 |
| adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial. | to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients. | 2012 | 22445846 |
| analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence. | to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences. | 2013 | 24278920 |
| relationship between serologic markers of periodontal bacteria and metabolic syndrome and its components. | periodontitis is a result of a complex biologic alteration of the periodontal microenvironment and a distributional shift of key periodontal pathogens. metabolic syndrome (mets), a complex cluster of cardiovascular risk factors, has been linked to periodontal diseases; however, the contribution of periodontal bacteria to systemic conditions remains unclear. | 2015 | 25415246 |
| the effects of different orthodontic appliances upon microbial communities. | orthodontic appliances can promote accumulation of dental plaque, with associated enamel decalcification or gingival inflammation. the aim of this study was to examine longer-term microbiological changes during orthodontic treatment with fixed appliances. | 2014 | 24345204 |
| microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. | the detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. the collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. however, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. the aim of this cross-sectional s ... | 2014 | 24144271 |
| comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample? | the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control). | 2017 | 27786617 |
| does obesity influence the subgingival microbiota composition in periodontal health and disease? | to evaluate whether obesity affects the subgingival microbial composition of patients with periodontal health or chronic periodontitis (cp). | 2016 | 27717180 |
| detection of subgingival periodontal pathogens--comparison of two sampling strategies. | the aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant. | 2016 | 26193958 |
| tlr4, nod1 and nod2 mediate immune recognition of putative newly identified periodontal pathogens. | periodontitis is a polymicrobial inflammatory disease that results from the interaction between the oral microbiota and the host immunity. although the innate immune response is important for disease initiation and progression, the innate immune receptors that recognize both classical and putative periodontal pathogens that elicit an immune response have not been elucidated. by using the human oral microbe identification microarray (homim), we identified multiple predominant oral bacterial speci ... | 2016 | 26177212 |
| subgingival microbial profiles of generalized aggressive and chronic periodontal diseases. | the aim of this study was to distinguish between generalized aggressive (gagp) and chronic periodontitis (cp) based on the subgingival microbial profiles predominant in these diseases. | 2012 | 22377404 |
| influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes. | this study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (dm) and generalized chronic periodontitis (chp). | 2017 | 28198980 |
| effect of smokeless tobacco products on human oral bacteria growth and viability. | to evaluate the toxicity of smokeless tobacco products (stps) on oral bacteria, seven smokeless tobacco aqueous extracts (staes) from major brands of stps and three tobacco-specific n-nitrosamines (tsnas) were used in a growth and viability test against 38 oral bacterial species or subspecies. all seven staes showed concentration-dependent effects on the growth and viability of tested oral bacteria under anaerobic culture conditions, although there were strain-to-strain variations. in the presen ... | 2016 | 27756619 |
| effect of photoactivated disinfection using light in the blue spectrum. | light in the blue spectrum is well known to cure composites. this in vitro-study was aimed to analyze a potential antimicrobial activity when combined with riboflavin as a photosensitizer. | 2016 | 26994334 |
| subgingival bacterial recolonization after scaling and root planing in smokers with chronic periodontitis. | the aim of this study was to compare subgingival bacterial recolonization patterns after scaling and root planing in current smokers and non-smokers. | 2015 | 25283721 |
| metronidazole and amoxicillin as adjuncts to scaling and root planing for the treatment of type 2 diabetic subjects with periodontitis: 1-year outcomes of a randomized placebo-controlled clinical trial. | to evaluate the clinical and microbiological effects of the use of metronidazole (mtz) + amoxicillin (amx) as adjuncts to scaling and root planing (srp) for the treatment of chronic periodontitis (chp) in type 2 diabetic subjects. | 2014 | 24930639 |
| inter-bacterial correlations in subgingival biofilms: a large-scale survey. | although the complexity of the oral ecology and the ecological differences between health and disease are well accepted, a clear view on the dynamics in relation to disease is lacking. in this study, the prevalence and abundance of 20 key oral bacteria was assessed in health and disease and more importantly a closer look was given to the inter-bacterial relationships. | 2014 | 24102517 |