| antibodies in normal and diseased pulps reactive with microorganisms isolated from deep caries. | immunoglobulin molecules in the supernatant fluids (sf) from pulpal explant cultures have been observed to react with microorganisms implicated in infections of root canals. in this study, the reactivity of immunoglobulin molecules in the sf from normal and irreversible pulpitis pulps to six strains of predominant microorganisms isolated from the immediate layer of carious lesions above the pulps used for explant cultures was investigated using an enzyme-linked immunosorbent assay. two atcc stra ... | 1992 | 1402561 |
| coaggregation studies of the eubacterium species. | eubacterium species are gram-positive anaerobic rods that are frequently isolated from subgingival plaque of periodontal pockets. five eubacterium species were tested for their ability to coaggregate with 33 oral bacterial strains. using visual and turbidimetric assays, coaggregation was observed among eubacterium brachy, eubacterium nodatum, eubacterium alactolyticum and eubacterium limosum strains only when tested with fusobacterium nucleatum strains; eubacterium saburreum displayed only weak ... | 1992 | 1494452 |
| serum antibodies to periodontal bacteria. | the purpose of this study was to determine how serum antibodies reactive with periodontitis-associated bacteria with relates to the diagnosis of periodontitis subjects. study groups included localized juvenile periodontitis (ljp) subjects, severe periodontitis (sp) subjects, chronic adult periodontitis (ap) subjects, and age matched controls. twenty-two bacterial strains, representing 18 different species most commonly found in early onset periodontitis were evaluated using serum from ljp, sp, a ... | 1990 | 2117654 |
| systemic antibody response of clinically characterized patients with antigens of eubacterium brachy initially and following periodontal therapy. | eubacterium brachy, a gram-positive anaerobic rod, has been implicated by cultural studies to be associated with the microflora of periodontal diseases. serum samples from 184 clinically characterized patients were evaluated in a standardized enzyme-linked immunosorbent assay (elisa) for reactivity to e. brachy antigens. sera from clinically healthy subjects (hs) served as controls. sera from rapidly progressive periodontitis (rp) patients demonstrated significantly greater reactivity by elisa t ... | 1986 | 3464734 |
| characteristics and sites of infection of eubacterium nodatum, eubacterium timidum, eubacterium brachy, and other asaccharolytic eubacteria. | three new species, eubacterium nodatum, eubacterium timidum, and eubacterium brachy, were described, primarily from subgingival samples taken from patients with moderate and severe adult periodontitis. except for the isolation of e. brachy from a pleuropulmonary infection, these species have not been reported from other infected body sites. we report on the isolation of these species and an undescribed group (d-6) of asaccharolytic eubacteria also found in periodontal disease from numerous diffe ... | 1987 | 3624445 |
| reaction of human sera with eubacterium brachy: isolation and characterization of an extracellular antigen. | recent studies have demonstrated an association of eubacterium sp. with the subgingival microflora of patients with chronic periodontitis. one species, eubacterium brachy, was evaluated to determine the possible mechanisms by which this microorganism may contribute to these diseases. of 167 sera evaluated by double diffusion in agar, 20.8% displayed reactivity with a sonicated preparation of e. brachy. an extracellular antigen was identified in the culture supernatant fluid which reacted with an ... | 1985 | 3972441 |
| serum antibody reactive with predominant organisms in the subgingival flora of young adults with generalized severe periodontitis. | in the present study we sought to determine whether serum antibody was present against microorganisms which predominate in the subgingival flora of young adults with generalized severe periodontitis (sp). subjects with sp were often seropositive for eubacterium brachy, fusobacterium nucleatum e3c22, and peptostreptococcus micros, whereas subjects with juvenile periodontitis (jp) and subjects with healthy periodontium (hp) were not. both sp and jp subjects were more frequently seropositive for ba ... | 1985 | 3988337 |
| eubacterium brachy. reactivity in in vitro bone resorptive bioassay. | recent studies have demonstrated an association of eubacterium sp. with the subgingival microflora of patients with chronic periodontitis. one species, eubacterium brachy, was evaluated to determine the possible mechanisms by which this microorganism may contribute to this disease. an extracellular antigen was identified in the culture supernatant which reacted with antibodies in human sera. this antigen was isolated by methanol precipitation and purified by gel filtration. the purified extracel ... | 1984 | 6423804 |
| pleuropulmonary infection associated with eubacterium brachy, a new species of eubacterium. | a new species of eubacterium was isolated from a case of pleural effusion. a case history and description of the organism are given. | 1980 | 7276145 |
| the metabolism of phenylalanine and leucine by a cell suspension of eubacterium brachy and the effects of metronidazole on metabolism. | degradation of phenylalanine and leucine by resting cells of eubacterium brachy atcc 33089 were studied under strict anaerobic conditions. the effects of metronidazole and air on the metabolism were also studied. the organism principally produced phenylpropionate and isocaproate from phenylalanine and leucine, respectively. other products were cinnamate and phenylacetate from phenylalanine, and alpha-ketoisocaproate and isovalerate from leucine. the organism also produced hydroxylated end-produc ... | 1994 | 7695510 |
| phylogeny of oral asaccharolytic eubacterium species determined by 16s ribosomal dna sequence comparison and proposal of eubacterium infirmum sp. nov. and eubacterium tardum sp. nov. | 16s rrna gene sequences of eubacterium brachy, eubacterium nodatum, eubacterium saphenum, eubacterium timidum, and two previously unnamed taxa were determined. the results of a phylogenetic analysis indicated that all of the strains sequenced belonged to a deep branch of the low-g+c-content gram-positive group. the levels of 16s ribosomal dna sequence similarity between species were low, suggesting that a number of genera may be represented in this group. the representatives of the two unnamed t ... | 1996 | 8863423 |
| restriction fragment-length polymorphism analysis of 16s rdna from oral asaccharolytic eubacterium species amplified by polymerase chain reaction. | restriction fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic eubacterium species, that is, eubacterium brachy, eubacterium exiguum, eubacterium lentum, eubacterium minutum, eubacterium nodatum, eubacterium saphenum, eubacterium timidum and 33 asaccharolytic eubacterium strains isolated from oral sites. the 16s rrna gene sequences from isolated genomic dna samples we ... | 1998 | 9573818 |
| cryptobacterium curtum gen. nov., sp. nov., a new genus of gram-positive anaerobic rod isolated from human oral cavities. | novel eubacterium-like isolates, strains 12-3t and kv43-b, which were isolated from the periodontal pocket of an adult patient with periodontal disease and necrotic dental pulp, respectively, were studied taxonomically and phylogenetically. the morphological and differential biochemical characteristics of these organisms are also described in this paper. these organisms were gram-positive, anaerobic, non-spore-forming, rod-shaped bacteria that were inert in most of the conventional biochemical t ... | 1999 | 10425779 |
| description of mogibacterium pumilum gen. nov., sp. nov. and mogibacterium vescum gen. nov., sp. nov., and reclassification of eubacterium timidum (holdeman et al. 1980) as mogibacterium timidum gen. nov., comb. nov. | a new genus, mogibacterium, is proposed for anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria which have been isolated from the periodontal pockets of adult human patients with periodontal disease and infected root canals. the novel isolates, strains d2-18t, ba11a-f and d5-2t, were inert in most of the conventional biochemical tests and phenotypically resemble asaccharolytic eubacterium species. the protein profiles of whole cells on sds-page gels and western immunoblotting reacti ... | 2000 | 10758875 |
| taxonomic characterization of mogibacterium diversum sp. nov. and mogibacterium neglectum sp. nov., isolated from human oral cavities. | novel isolates, strains hm-7, hm-6, hh-31, p9a-ht and ujb13-d, which were isolated from tongue plaque and necrotic dental pulp, were studied taxonomically and phylogenetically. these organisms were anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria that were inert in most of the conventional biochemical tests and phenotypically resemble mogibacterium species or asaccharolytic eubacterium species. the g+c contents of the dnas from the novel isolates ranged from 41 to 42 mol %. dna-d ... | 2002 | 11837293 |
| campylobacter species isolated from extra-oro-intestinal abscesses: a report of four cases and literature review. | campylobacter species are frequently isolated from fecal specimens of patients with diarrheal illness. several campylobacter species are commonly isolated from the oral cavity. in contrast, campylobacter species are rarely isolated from extra-oro-intestinal abscesses. reported here are four cases of extra-oro-intestinal abscesses due to polymicrobial flora, including campylobacter species. the first case is a 35-year-old woman who was diagnosed with a brain abscess caused by c. gracilis, strepto ... | 2008 | 18488257 |
| subgingival microbial communities in leukocyte adhesion deficiency and their relationship with local immunopathology. | leukocyte adhesion deficiency i (lad-i) is a primary immunodeficiency caused by single gene mutations in the cd18 subunit of β2 integrins which result in defective transmigration of neutrophils into the tissues. affected patients suffer from recurrent life threatening infections and severe oral disease (periodontitis). microbial communities in the local environment (subgingival plaque) are thought to be the triggers for inflammatory periodontitis, yet little is known regarding the microbial comm ... | 2015 | 25741691 |
| molecular methods for diagnosis of odontogenic infections. | historically, the identification of microorganisms has been limited to species that could be cultured in the microbiology laboratory. the purpose of the present study was to apply molecular techniques to identify microorganisms in orofacial odontogenic infections (ois). | 2012 | 22326175 |
| microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. | this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp). | 2015 | 26521147 |
| a case of relapsed lung abscess caused by eubacterium brachy infection following an initial diagnosis of pulmonary actinomycosis. | we report a rare case of lung abscess due to eubacterium brachy. in this case, an analysis of the aspirate from frank pus revealed gram-positive coccobacilli. we initially strongly suspected lung abscess associated with actinomycosis because of the chronic/recurrent clinical course and radio-pathological findings such as a granuloma lesion. although a biochemical analysis revealed actinomyces sp., 16s rrna gene sequencing and a phylogenetic tree analysis of the isolated strain confirmed the pres ... | 2017 | 28856090 |