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cross-neutralization study of seven california group (bunyaviridae) strains in homoiothermous (ps) and poikilothermous (xtc-2) vertebrate cells.antigenic relationships among seven california group strains were studied by a plaque-reduction neutralization test (prnt). cross-reactions occurred in most cases but three subgroups were noted: (1) the major serogroup contained the viruses of california encephalitis, lacrosse, snowshoe hare and trahyna (including the lumbo strain) whereas (2) jamestown canyon and (3) trivittatus viruses were distinct. there was no significant difference between the prnt results in mammalian (ps) cells incubated ...197984857
arbovirus vector ecology studies in mexico during the 1972 venezuelan equine encephalitis outbreak.virus vector studies were conducted in the states of durango, chihuahua, and tamaulipas, mexico, in june and july 1972. apparently only a low level of venzuelan equine encephalitis (vee) virus transmission to equines occured at the time of the study, and the infection was restricted to areas which had not experienced overt activity during the preceding year. the low level of infection was associated with a scarcity of mosquitoes. the ib (epidemic) strain of vee virus was isolated from two pools ...1975235213
laboratory studies of transovarial transmission of trivittatus virus by aedes trivittatus.aedes trivittatus were experimentally exposed through a membrane to a concentration of trivittatus (tvt) virus in dog defibrinated blood of 10(3.4)-10(3.5) smicld 50/0.025 ml. transovarial and transstadial transmission was demonstrated. fifty-five percent of the infected mosquitoes tested transovarially transmitted tvt virus. tvt virus was isolated from 16% of the f1 generation progeny examined, including 19% of the larvae, 23% of adult females, and 10% of adult males.1978626271
isolation of trivittatus virus from larvae and adults reared from field-collected larvae of aedes trivittatus (diptera: culicidae). 1977886560
trivittatus virus infections in wild mammals and sentinel rabbits in central iowa.a serological survey was conducted in iowa to determine the prevalence rate of california group virus antibodies in sera of several vertebrate species. serum specimens were assayed for infectivity-neutralizing antibody in a microneutralization system with baby hamster kidney cell culture. of 77 sera assayed, 21 (27%) neutralized trivittatus (tvt) virus infectivity. the antibody prevalence rate was highest for eastern cottontail rabbits inasmuch as 46% (10/22) of the serum specimens form this spe ...19751200251
experimental transmission of trivittatus virus (california virus group) by aedes trivittatus.the mosquito, aedes trivittatus, when fed through a membrane a trivittatus virus dosage of 10(3.4) to 10(5.5) suckling mouse ld50/0.03 ml of blood, transmitted the virus to suckling mice. virus multiplication indicative of a biological vector occurred in this species. when aedes vexans and a. triseriatus ingested similar doses of trivittatus virus, both the infection and transmission rates were low and virus multiplication was poor. these results, added to evidence based on virus isolations from ...19761259079
isolation of jamestown canyon virus (california serogroup) from aedes mosquitoes in an enzootic focus in michigan.twenty isolates of jamestown canyon virus were obtained from adult females of 5 aedes species collected at the houghton lake wildlife research area, missaukee county, in north-central michigan between 1985 and 1989. fourteen were from aedes provocans, and 6 were from 4 other snowmelt aedes species. one isolate of trivittatus virus and one cache valley-like virus were also obtained. seasonal succession patterns for numerous mosquito species were recorded over 4 years. the temporal association of ...19902230774
serum antibody prevalence for herpesvirus sylvilagus, bacillus piliformis and california serogroup arboviruses in cottontail rabbits from pennsylvania.a serologic survey of 60 eastern cottontail rabbits (sylvilagus floridanus) from three counties in pennsylvania was conducted in march 1983. serum antibody prevalences for herpesvirus sylvilagus and la crosse virus (california serogroup) were less than 4%. there was no evidence of previous exposure to either jamestown canyon or snowshoe hare viruses (california serogroup). antibody to trivittatus virus (california serogroup) was found in 60% of the 20 cottontails from york county. no cottontails ...19883373643
spontaneous flight activity of aedes trivittatus (diptera: culicidae) infected with trivittatus virus (bunyaviridae: california serogroup). 19873585922
seroconversion rates to jamestown canyon virus among six populations of white-tailed deer (odocoileus virginianus) in indiana.the annual seroconversion of fawns, yearlings, and adult white-tailed deer (odocoileus virginianus) to jamestown canyon virus (california group) was followed at six indiana sites from 1981 through 1984. in all, sera from 1,642 deer (515 fawns, 618 yearlings, and 509 adults) were tested for neutralizing antibody to three california serogroup viruses: jamestown canyon, la crosse, and trivittatus. virtually all deer with specific neutralizing antibody showed evidence of a prior infection with james ...19873820426
virus isolations from mosquitoes in southern ontario, 1976 and 1977.following the 1975 epidemic of st. louis encephalitis (sle) in ontario, programs were instituted to monitor virus activity in mosquito populations during 1976 and 1977. mosquitoes were trapped with cdc light traps and co2 cone traps, pooled by species, and tested for virus by intracerebral inoculation of suckling mice. in 1976, 51 175 mosquitoes were tested. sle virus was isolated from two mixed pools of culex pipiens--c. restuans mosquitoes. five isolations of california serogroup viruses were ...19806103747
isolations of potosi virus from mosquitoes (diptera: culicidae) collected in connecticut.potosi virus (potv) (bunyaviridae: orthobunyavirus) was first isolated from aedes albopictus (skuse) collected in potosi, mo, in 1989, and subsequent isolations were reported from illinois, michigan, ohio, and the carolinas. to determine whether the distribution of this virus extends into the northeastern united states, we analyzed arboviruses acquired from mosquitoes collected in connecticut from 1998 to 2004. in 2001, a bunyavirus was isolated from aedes vexans (meigen) that was different from ...200516363172
complete genome sequence of trivittatus virus.trivittatus virus (family bunyaviridae, genus orthobunyavirus) represents an important genetic intermediate between the california encephalitis group and the bwamba/pongola and nyando groups. here, we report the first complete genome sequence of the prototype (eklund) strain, isolated in 1948, which, interestingly, shows only a few differences when compared to partial sequences of modern strains.201526212363
isolations of trivittatus virus from wisconsin mosquitoes, 1964-1968. 19724401663
serological survey for antibodies to mosquito-borne bunyaviruses among us national park service and us forest service employees.serum samples from 295 employees of great smoky mountains national park (grsm), rocky mountain national park (romo), and grand teton national park with adjacent bridger-teton national forest (grte-btnf) were subjected to serological analysis for mosquito-borne bunyaviruses. the sera were analyzed for neutralizing antibodies against six orthobunyaviruses: la crosse virus (lacv), jamestown canyon virus (jcv), snowshoe hare virus (sshv), california encephalitis virus, and trivittatus virus (tvtv) b ...201626855300
arboviruses in north dakota, 2003-2006.to investigate arbovirus transmission in north dakota, we collected and screened mosquitoes for viral infection by vero cell culture assay. seven viruses were isolated from 13 mosquito species. spatial and temporal distributions of the important vectors of west nile virus (wnv), cache valley virus, jamestown canyon virus (jcv), and trivittatus virus are reported. snowshoe hare virus, potosi virus, and western equine encephalomyelitis virus were also isolated. the risks of culex tarsalis and aede ...201425487728
determination and comparative analysis of the small rna genomic sequences of california encephalitis, jamestown canyon, jerry slough, melao, keystone and trivittatus viruses (bunyaviridae, genus bunyavirus, california serogroup).the nucleotide sequences of the small (s) genomic rnas of six california (cal) serogroup bunyaviruses (bunyaviridae: genus bunyavirus) were determined. the s rnas of two california encephalitis virus strains, two jamestown canyon virus strains, jerry slough virus, melao virus, keystone virus and trivittatus virus contained the overlapping nucleocapsid (n) and non-structural (nss) protein open reading frames (orfs) as described previously for the s rnas of other cal serogroup viruses. all n prote ...19957897347
jamestown canyon virus (california serogroup) is the etiologic agent of widespread infection in michigan humans.in a sample population of 780 michigan residents tested for neutralizing antibodies to california serogroup viruses, 216 (27.7%) had specific neutralizing antibody to jamestown canyon virus. an additional eight (1.0%) had specific neutralizing to trivittatus virus; none had specific neutralizing antibody to la crosse virus. significantly more male residents than female residents of the lower peninsula had antibody to jamestown canyon virus. the frequency of neutralizing antibody titers fits the ...19863953951
virus-vector-host relationships of aedes stimulans and jamestown canyon virus in a northern indiana enzootic focus.collections of hematophagous diptera at the kingsbury state fish and wildlife area in northern indiana between 1982 and 1984 yielded 118,972 mosquitoes from which 5 isolates of jamestown canyon virus and 3 isolates of trivittatus virus were obtained. all jamestown canyon isolates were from aedes stimulans, including 1 from a pool of newly emerged males and 2 from pools of newly emerged females. these 3 isolates suggest that jamestown canyon virus is transovarially transmitted by ae. stimulans. a ...19862878626
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