| plasmids containing cos ends inhibit the replication of phage phi ctx in pseudomonas aeruginosa. | in bacteriophage phi ctx, the cohesive end sequences cos, the integrase gene int, the attachment site attp (the target site for int) and the gene ctx encoding a pore-forming cytotoxin ctx, are clustered. phi ctx can infect some pseudomonas aeruginosa strains with a subsequent induction of ctx expression. the 41 and 477 bp fragments containing cos ends of phi ctx dna were cloned into the high copy number plasmid pha10. after pretransformation with the cos ends containing plasmids, plaque formatio ... | 1996 | 8725104 |
| control of effective expression of the phage phictx-encoded ctx gene in pseudomonas aeruginosa by a promoter upstream of the cos site. | in the dna of bacteriophage phictx, the attachment site (attp), the cohesive end site (cos), and the gene (ctx) encoding the pore-forming cytotoxin, are clustered. deletion variants and pcr-generated fragments of the dna were cloned into the pseudomonas aeruginosa and escherichia coli vector pha10. recombinant plasmids carrying the chloramphenicol acetyltransferase gene, cat, were used for promoter studies. two promoters responsible for ctx expression are located between attp and cos and between ... | 1998 | 9520258 |
| mutational analysis of bacteriophage phi ctx cos site. | the dna of phi ctx contains the gene ctx, located closely downstream of cos. this encodes for the pore-forming cytotoxin protein, ctx. phi ctx converts some pseudomonas aeruginosa strains into ctx producers. after different periods of phi ctx infection, two distinct forms of phage dna were isolated: circular dna from bacterial cytosol and later linear dna from phi ctx particles. when circular phi ctx dna was transfected into the p. aeruginosa strains cf5 and e40, phi ctx was amplified and ctx ex ... | 1996 | 9029787 |