| watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan. | the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ... | 2011 | 21399920 |
| complete nucleotide sequence of watermelon chlorotic stunt virus originating from oman. | watermelon chlorotic stunt virus (wmcsv) is a bipartite begomovirus (genus begomovirus, family geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the middle east and north africa. recently squash (cucurbita moschata) grown in an experimental field in oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence iden ... | 2012 | 22852046 |
| molecular characterization of watermelon chlorotic stunt virus (wmcsv) from palestine. | the incidence of watermelon chlorotic stunt disease and molecular characterization of the palestinian isolate of watermelon chlorotic stunt virus (wmcsv-[pal]) are described in this study. symptomatic leaf samples obtained from watermelon citrullus lanatus (thunb.), and cucumber (cucumis sativus l.) plants were tested for wmcsv-[pal] infection by polymerase chain reaction (pcr) and rolling circle amplification (rca). disease incidence ranged between 25%-98% in watermelon fields in the studied ar ... | 2014 | 24956181 |
| analysis of watermelon chlorotic stunt virus and tomato leaf curl palampur virus mixed and pseudo-recombination infections. | watermelon chlorotic stunt virus (wmcsv) and tomato leaf curl palampur virus (tolcpmv) are limiting factors for cucurbit production in south and southeastern iran. tolcpmv infects all cucurbit crops (except watermelons) whereas wmcsv is somewhat limited to watermelon, causing detrimental effects on fruit production. in a survey, we detected wmcsv in all watermelon growing farms in fars province (southern iran). given that wmcsv and tolcpmv are present in the same geographical location in iran, w ... | 2015 | 26433951 |
| frequent migration of introduced cucurbit-infecting begomoviruses among middle eastern countries. | in the early 2000s, two cucurbit-infecting begomoviruses were introduced into the eastern mediterranean basin: the old world squash leaf curl virus (slcv) and the new world watermelon chlorotic stunt virus (wmcsv). these viruses have been emerging in parallel over the last decade in egypt, israel, jordan, lebanon and palestine. | 2014 | 25300752 |
| impact of cis-acting elements' frequency in transcription activity in dicot and monocot plants. | the production of new cultivars via recombinant dna technology is important in applied agriculture. promoters play fundamental roles in successful transformation and gene expression. fragments of the upstream regulatory region of the movement protein gene of the tomato yellow leaf curl virus (tylcv; two fragments) and watermelon chlorotic stunt virus (wmcsv, two fragments) and one fragment of the coat protein putative promoter of tylcv (cpty-pro) were isolated to assess their abilities to drive ... | 2015 | 28324408 |
| implication of bemisia tabaci heat shock protein 70 in begomovirus-whitefly interactions. | the whitefly bemisia tabaci (gennadius) is a major cosmopolitan pest capable of feeding on hundreds of plant species and transmits several major plant viruses. the most important and widespread viruses vectored by b. tabaci are in the genus begomovirus, an unusual group of plant viruses owing to their small, single-stranded dna genome and geminate particle morphology. b. tabaci transmits begomoviruses in a persistent circulative nonpropagative manner. evidence suggests that the whitefly vector e ... | 2012 | 23015709 |
| quantification and localization of watermelon chlorotic stunt virus and tomato yellow leaf curl virus (geminiviridae) in populations of bemisia tabaci (hemiptera, aleyrodidae) with differential virus transmission characteristics. | bemisia tabaci (gennadius) is one of the economically most damaging insects to crops in tropical and subtropical regions. severe damage is caused by feeding and more seriously by transmitting viruses. those of the genus begomovirus (geminiviridae) cause the most significant crop diseases and are transmitted by b. tabaci in a persistent circulative mode, a process which is largely unknown. to analyze the translocation and to identify critical determinants for transmission, two populations of b. t ... | 2014 | 25365330 |
| characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: squash leaf curl virus and watermelon chlorotic stunt virus. | squash leaf curl virus (slcv) and watermelon chlorotic stunt virus (wmcsv) are cucurbit-infecting bipartite begomoviruses. both viruses are found in the eastern mediterranean basin but the effects of dual infection of both viruses on melon (cucumis melo l.) have not been described. 'arava' melon plants were inoculated in the greenhouse, using whiteflies, with either slcv, wmcsv, or both. control plants were exposed to nonviruliferous whiteflies or not exposed at all. following inoculation, plant ... | 2011 | 21219130 |
| inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling circle amplification of total dna from infected plants and whiteflies. | a new system for inoculation of plants with begomoviral dna without cloning or the use insect vectors is described. total dna extracted from begomovirus-infected plants was amplified by rolling circle amplification (rca) using the bacteriophage phi29 dna polymerase, and inoculated to plants by particle bombardment. infection rates of up to 100% were obtained using this technique. this technique successfully inoculated all the begomoviruses evaluated: five bipartite (bean golden yellow mosaic vir ... | 2010 | 20447420 |
| watermelon chlorotic stunt virus from the sudan and iran: sequence comparisons and identification of a whitefly-transmission determinant. | abstract the genomes of two watermelon chlorotic stunt virus (wmcsv) isolates, one from the sudan and one from iran, were cloned and sequenced. sequence relationship with other geminiviruses characterizes wmcsv as a typical eastern hemisphere geminivirus with a bipartite genome. the two geographically distant wmcsv isolates from africa and the middle east share a very high overall sequence similarity: 98% between their dna-a and 96% between their dna-b components, and their respective capsid pro ... | 2000 | 18944543 |
| occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran. | during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ... | 2008 | 18564898 |