| structure, restriction map and infectivity of the genomic and replicative forms of aapv dna. | we have characterized the genomic and replicative form (rf) dna of the aedes albopictus parvovirus (aapv), a virus isolated from a chronically infected c6/36 clone of aedes albopictus cell line [22]. the genome of aapv virions is a single-stranded linear dna molecule approximately 4.2 kb in length, essentially (about 90%) encapsidated as minus strand. a restriction map of the rf dna isolated from infected c6/36 cells was established. among the 23 restriction enzymes tested, 14 cleaved the aapv r ... | 1994 | 7944946 |
| complete nucleotide sequence and genomic organization of the aedes albopictus parvovirus (aapv) pathogenic for aedes aegypti larvae. | we have cloned the replicative form of the aedes albopictus parvovirus (aapv) genome and determined the complete sequence of the viral strand. the sequence is 4176 nucleotides (nt) in length. the first 134 nt at the 3' end and the terminal 182 nt at the 5' end of the viral (minus) strand can both generate by folding and annealing of complementary sequences a typical terminal t-shaped structure although they differ in their sequence. three large open reading frames (orfs), each one in a different ... | 1994 | 8178459 |
| pathogenicity of the aedes albopictus parvovirus (aapv), a denso-like virus, for aedes aegypti mosquitoes. | aapv, a denso-like virus isolated from a c6/36 clone of the aedes albopictus cell line, proved to be very pathogenic for aedes aegypti first and third instar larvae following per os infection. the mortality reached 90% in 10 days for larvae infected at the first instar. several factors, such as temperature, larval density and stage, and duration of contact with infectious particles, influenced infection. the virus titer in females surviving infection at the third larval instar reached 10(8) tcid ... | 1996 | 8931366 |
| venereal and vertical transmission of the aedes albopictus parvovirus in aedes aegypti mosquitoes. | following per oral infection of aedes aegypti larvae with aedes albopictus parvovirus (aapv), infected males and females adults were tested for their ability to transmit the virus venereally and vertically, respectively. both types of transmission were observed. a low percentage (2.2%) of aapv-free females were found contaminated by the virus after mating with aapv-infected males. although no significant difference was observed in the fecundity of orally infected and virus-free females, 17.1% of ... | 1997 | 9288802 |
| a new densovirus isolated from the mosquito culex pipiens (diptera: culicidae). | we have isolated from a laboratory strain of culex pipiens in which abnormal larval mortality occurred a small icosahedral nonenveloped dna virus sharing the main biological and biophysical properties of densoviruses (dnvs). unlike dnvs isolated previously from aedes species, i.e. the aaednv and the aaldnv (afanasiev, b.n., galyov, e. e., buchatsky, l.p., kozlov, y.v., 1991. nucleotide sequence and genomic organization of aedes densonucleosis virus. virology 185, 323-336; boublik, y., jousset, f ... | 2000 | 10773314 |
| infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses. | we purified and sequenced infectious hypodermal and hematopoietic necrosis virus (ihhnv), a small dna virus of shrimp, from wild penaeus stylirostris. the virion has a buoyant density of 1.45 as determined by cesium chloride gradient. analysis of 3873 nucleotides of the viral genome revealed three large open reading frames (orfs) and parts of the noncoding termini of the viral genome. the left, mid, and right orfs on the complementary (plus) strand have potential coding capacities of 666 amino a ... | 2000 | 11062047 |
| comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groups. | the dna and putative amino acid sequences of representative insect and shrimp parvoviruses (subfamily densovirinae) were analyzed using computer programs. shrimp viruses included hepatopancreatic parvovirus (hpv) of penaeus monodon (hpvmon) and p. chinensis (hpvchin), spawner-isolated mortality virus from p. monodon (smvmon) and infectious hypodermal and hematopoietic necrosis virus (ihhnv) from p. vannamei. insect viruses included aedes aegypti densovirus (aaednv), aedes albopictus densovirus ( ... | 2002 | 12135792 |
| structure comparisons of aedes albopictus densovirus with other parvoviruses. | parvoviridae is a family of the smallest viruses known with a wide variety of hosts. the capsid structure of the aedes albopictus c6/36 cell densovirus (c6/36 dnv) at 1.2-nm resolution was obtained by electron cryomicroscopy (cryoem) and three-dimensional (3d) image reconstruction. structure comparisons between the c6/36 dnv and other parvoviruses reveal that the degree of structural similarity between c6/36 dnv and the human parvovirus b19 is higher than that between c6/36 dnv and other insect ... | 2007 | 17393085 |
| a new densovirus isolated from the mosquito toxorhynchites splendens (wiedemann) (diptera: culicidae). | a new densovirus was isolated and characterized in laboratory strains of toxorhynchites splendens. the virus was detected by polymerase chain reaction (pcr) from mosquitoes reared in our laboratory. pcr fragments from each mosquito were compared by single strand conformation polymorphism (sscp) assay and found to be indistinguishable. thus, it is likely the densoviruses from these mosquitoes contain homologous nucleotide sequences. the pcr fragment corresponding to a 451 bp densovirus structural ... | 2007 | 17539278 |
| isolation and characterization of the full coding sequence of a novel densovirus from the mosquito culex pipiens pallens. | during an investigation of arboviruses in china, a novel densovirus (dnv) was isolated from the adult female culex pipiens pallens. the virus, designated culex pipiens pallens densovirus (cppdnv), caused cytopathic effect in c6/36 cells. the virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. the complete coding region of cppdnv was found to be 3335 nt and it contained three open reading frames (orfs). cppdnv shares 82-93 % identical nucleotides with isolates of the ... | 2008 | 18089743 |
| expression strategy of aedes albopictus densovirus. | the transcription map of the aedes albopictus densovirus (aaldnv) brevidensovirus was identified by northern blotting, rapid amplification of cdna ends (race) analysis, and rnase protection assays. aaldnv produced mrnas of 3,359 (ns1), 3,345 (ns2), and 1,246 (vp) nucleotides. the two overlapping p7/7.4 ns promoters employed closely located alternate transcription initiation sites, positioned at either side of the ns1 initiation codon. all ns mrnas coterminated with vp mrna. all promoters, explor ... | 2013 | 23804640 |
| application of post-pcr methods for analysis of mosquito densovirus. | two clades of aedes densovirus, aedes aegypti densovirus and aedes albopictus densovirus, were classified according to the origin of isolation. these two densoviruses were isolated from indigenous mosquitoes and mosquito cell lines, respectively. this group of invertebrate viruses belongs to the subfamily densovirinae of the parvoviridae family and infects only insects. several types of densoviruses have been isolated from mosquitoes especially aedes aegypti and aedes albopictus, which are impor ... | 2014 | 25507597 |
| application of post-pcr methods for analysis of mosquito densovirus. | two clades of aedes densovirus, aedes aegypti densovirus and aedes albopictus densovirus, were classified according to the origin of isolation. these two densoviruses were isolated from indigenous mosquitoes and mosquito cell lines, respectively. this group of invertebrate viruses belongs to the subfamily densovirinae of the parvoviridae family and infects only insects. several types of densoviruses have been isolated from mosquitoes especially aedes aegypti and aedes albopictus, which are impor ... | 2014 | 25427347 |
| comparison of mosquito densoviruses: two clades of viruses isolated from indigenous mosquitoes. | we analyzed the phylogenetic tree of densoviruses isolated from indigenous mosquitoes and mosquito cell lines. our findings suggest two distinct clades of densovirus. the viruses in the first clade were isolated from an indigenous mosquito which had the aedes aegypti densovirus (aaednv) as a representative virus. the other clade of viruses was isolated from mosquito indigenous cell line which had the aedes albopictus densovirus (aaldnv) as the representative virus. the origin of the two clades o ... | 2013 | 24050091 |
| new genetic variation of aedes albopictus densovirus isolated from mosquito c6/36 cell line. | densovirus (dnv) is a small single-stranded dna, non-enveloped virus belonging to the subfamily densovirinae of the parvoviridae family. this group of invertebrate viruses infects exclusively insects. two of the major densoviruses, aedes aegypti (aaednv) and ae. albopictus (aaldnv), infect mosquitoes that carry viruses responsible for two important public health diseases, namely, dengue hemorrhagic fever and yellow fever. the present study describes cloning, sequencing and phylogenetic analysis ... | 2012 | 23431818 |
| an efficient and easy method of infection of mosquito larvae from virus-contaminated cell cultures. | a new method for efficient infection of aedes aegypti larvae by the aedes albopictus densovirus, aapv is described. it consists of placing first or third instar larvae in culture flasks containing a chronically infected mosquito cell line. after 24 or 48 h of exposure to the contaminated culture, the larvae acquired the virus by feeding on infected cells. using this technique, up to 95% of first instar ae. aegypti larvae were found infected by the aapv. | 1994 | 7822456 |
| a novel densovirus isolated from the asian tiger mosquito displays varied pathogenicity depending on its host species. | mosquito-borne viral diseases (mbvds) continue to pose a significant global public health burden. mosquito control remains a core intervention strategy in integrated mosquito management (imm) programs to reduce the transmission of mbvds. mosquito densoviruses (mdvs) are mosquito-specific entomopathogenic viruses, and their attractive biological and pathogenic characteristics make mdvs potential biological control agents as alternatives to traditional chemical pesticides. however, different viral ... | 2019 | 31333635 |
| markedly reduced severity of dengue virus infection in mosquito cell cultures persistently infected with aedes albopictus densovirus (aaldnv). | aaldnv-infected c6/36 cells serially passaged for over 10 weeks showed a decline in percentage of anti-aaldnv-positive cells (apc) from an initial 92% to approximately 20%. cultures of persistent apc were indistinguishable from uninfected cultures by direct microscopy but most stained cells from early apc passages had enlarged nuclei with eosinophilic inclusions, while late apc passages had few and naive cells none. super challenge of persistent apc cultures did not increase percentage apc and s ... | 2004 | 15518806 |