| hemagglutinin activity of two distinct genogroups of feline and canine rotavirus strains. | genomes of hemagglutinating strains of feline and canine rotaviruses, were much more closely related to each other than to non-hemagglutinating strains. the cat2 feline rotavirus appears to derive from reassortment between hemagglutinating and non-hemagglutinating strains. | 1992 | 1309998 |
| genetic diversity and similarity among mammalian rotaviruses in relation to interspecies transmission of rotavirus. | to address the question whether there was any molecular evidence for interspecies transmission of rotaviruses from one animal species to another, genetic relationships among human and animal rotaviruses were examined by a series of hybridization experiments in which genomic rnas from 14 rotavirus strains derived from seven different host species were hybridized with the [32p]-labelled transcription probes prepared from 11 strains representing rotaviruses from those seven host species. in general ... | 1991 | 1656919 |
| experience with serotyping rotavirus strains by reverse transcription and two-step polymerase chain reaction with generic and type-specific primers. | six vp7 serotypes or g types (g1-g4, g8 and g9) occur in group a human rotaviruses. gouvea et al. recently reported a novel g-typing method based on reverse transcription (rt)-polymerase chain reaction (pcr) amplification of the vp7 gene with type-specific primers [gouvea, v. et al. (1990). journal of clinical microbiology 28, 276-82]. when we followed their protocol, 40 (89%) of 45 faecal rotavirus specimens were typed into g1-g4 and g9. the five specimens that were untypeable by the rt-pcr met ... | 1991 | 1724291 |
| molecular identification by rna-rna hybridization of a human rotavirus that is closely related to rotaviruses of feline and canine origin. | with a few exceptions subgroup i group a human rotavirus strains have short rna patterns, whereas most animal rotavirus strains belong to subgroup i and have long rna patterns. thus, new isolates of subgroup i human rotaviruses with long rna patterns are considered to have a high likelihood of being animal rotaviruses. a group of human rotaviruses represented by the au-1 strain has recently been shown to be genetically related to a feline rotavirus (frv-1) isolated in japan. a human rotavirus, s ... | 1990 | 2166076 |
| analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. | the sb-1a rotavirus recovered from a diarrheic piglet in the united states is a naturally occurring intertypic rotavirus. when studied by reciprocal neutralization tests, the sb-1a virus was similar, if not identical, to the porcine gottfried virus (serotype 4) and the porcine osu virus (serotype 5). analysis of reassortant viruses prepared from the sb-1a virus and the serotype 2 human ds-1 virus revealed that the antigenic specificity of the outer capsid protein vp3 of sb-1a was shared with the ... | 1987 | 2434522 |
| comparative studies of the antigenic polypeptide species vp4, vp6, and vp7 of three strains of bovine rotavirus. | three bovine rotavirus strains belonging to two distinct serotype groups, serotype 6 (ncdv and b641) and b223, distinct from the other six mammalian rotavirus serotypes but not yet assigned to a serotype group, were compared with each other and with canine rotavirus (k9, serotype 3) by studying the properties of their cognate polypeptide species vp4, vp6, and vp7. the three viruses showed distinct differences in the polyacrylamide gel electrophoretic migration rates of protein species vp4 and vp ... | 1989 | 2476464 |
| characterization of a canine rotavirus strain by neutralization and molecular hybridization assays. | a canine rotavirus, rs-15, previously isolated in japan was determined to be subgroup i and serotype 3. when compared with prototype human and animal rotavirus strains by rna-rna hybridization assay, the rs-15 strain showed a high degree of homology only with the canine cu-1 strain isolated in the united states of america, suggesting that canine rotaviruses constitute a distinct gene family, which we have elsewhere proposed to term "genogroup". | 1989 | 2548456 |
| plaque formation of canine rotavirus in cultures of gbk bovine cell line. | | 1988 | 2834598 |
| isolation and identification of caliciviruses from dogs with enteric infections. | caliciviruses were isolated from 7 dogs and 1 captured coyote with enteritis. there was a high fatality rate in dogs 4 to 16 weeks of age. the occurrence in these dogs of concurrent infection with known enteric pathogens such as salmonella sp, canine parvovirus, canine coronavirus, and canine rotavirus did not allow making any conclusions regarding the pathogenicity of this newly recognized calicivirus. the caliciviruses were characterized by electron microscopy and were further identified as be ... | 1985 | 2982299 |
| characterization of canine rotavirus rs 15 strain and comparison with other rotaviruses. | | 1985 | 2995714 |
| a scanning and transmission electron microscopic study of rotavirus-induced intestinal lesions in neonatal gnotobiotic dogs. | neonatal gnotobiotic dogs orally inoculated with canine rotavirus had ultrastructural changes limited to the jejunal and ileal regions of the small intestine. early scanning electron microscopic findings consisted of swollen villus epithelial cells, denuded foci on intestinal villi, and slight to moderate villus atrophy. later changes were slight villus atrophy with no denuded intestinal villi. transmission electron microscopic changes in villus epithelial cells from 12 to 48 hours post-inoculat ... | 1986 | 3018981 |
| characterization of a canine rotavirus hemagglutinin. | | 1986 | 3784211 |
| isolation and characterization of a canine rotavirus. | canine rotavirus particles were visualized by direct electron microscopy in the feces from a clinically normal dog. the virus was subsequently propagated in cell cultures; it was characterized and compared with rotaviruses from other species. replication of the virus in cell culture was found to be less dependent upon trypsin than that of human, bovine and porcine rotaviruses. reproducible, sharp-edged plaques of various sizes were produced by the canine rotavirus in an established cell line of ... | 1982 | 6179502 |
| serological comparison of canine rotavirus with various simian and human rotaviruses by plaque reduction neutralization and hemagglutination inhibition tests. | by the plaque reduction neutralization test, the cu-1 strain of canine rotavirus was similar, if not identical, to three strains (no. 14, no. 15, and p) of the tentatively designated third human rotavirus serotype. in addition, strain cu-1 demonstrated a one-way antigenic relationship with two other strains (m and b) of the third human rotavirus serotype. the cu-1 strain of canine rotavirus hemagglutinated human group o, rhesus monkey, dog, sheep, and guinea pig erythrocytes. a two-way antigenic ... | 1983 | 6190752 |
| isolation of a rotavirus from a newborn dog with diarrhea. | a rotavirus was isolated from a newborn dog that died after having clinical signs of diarrhea. virus particles with rotaviral morphologic features were observed by transmission electron microscopy in the intestinal homogenate collected at necropsy. cytopathic effects were observed, and rotaviral antigens were detected by indirect immunofluorescence in ma-104 monolayer cultures (a fetal rhesus macaque kidney cell) inoculated with intestinal homogenate. this rotavirus isolate, designated lsu 79c-3 ... | 1981 | 6266295 |
| isolation, propagation, and characterization of a second equine rotavirus serotype. | a rotavirus designated strain h-2 was isolated in primary african green monkey kidney cells from a foal with diarrhea. this cell culture-adapted strain was found to be similar, if not identical, to simian rotavirus (strains mmu18006 and sa-11) and canine rotavirus (strain cu-1) and, in addition, demonstrated a one-way antigenic relationship with five human rotavirus strains (p, b, no. 14, no. 15, and yo) of the third human rotavirus serotype by the plaque reduction neutralization test. this is t ... | 1983 | 6309657 |
| isolation and characterization of an equine rotavirus. | a rotavirus, designated as the h-1 strain, was isolated from a diarrheic foal in primary african green monkey kidney cells and ma104 cells. this cell culture-adapted strain hemagglutinated erythrocytes of human group o, rhesus monkeys, guinea pigs, and sheep. it was found to be similar, if not identical, to porcine rotaviruses (strains osu, ee, and a-580) by plaque reduction neutralization and hemagglutination inhibition tests, and, in addition, it was found to belong to subgroup 1. this equine ... | 1983 | 6313746 |
| canine viral enteritis. | canine viral enteritis should be suspected in dogs with an acute onset of vomiting and diarrhea, especially in puppies and where several animals are affected simultaneously. definitive diagnosis requires laboratory confirmation, most often detection of viral particles in the stool. no diagnostic test is entirely specific or absolutely sensitive, however, and laboratory findings should be weighed accordingly. immunization is the key to successful control. effective vaccines for canine parvovirus ... | 1983 | 6316616 |
| inoculation of neonatal gnotobiotic dogs with a canine rotavirus. | neonatal gnotobiotic dogs were inoculated orally with a rotavirus isolated from a pup with fatal diarrhea, and in the gnotobiotic dogs, diarrhea was observed between postinoculation hours (pih) 20 and 24. the diarrhea persisted through pih 154, and inoculated pups had clinical signs of dehydration after pih 24. negative-contrast electron microscopy of the feces from inoculated pups revealed rotavirus particles from pih 12 through 154. using an indirect-fluorescent antibody test, serum rotavirus ... | 1983 | 6625322 |
| gross and light microscopic lesions in neonatal gnotobiotic dogs inoculated with a canine rotavirus. | gross, light microscopic, and morphometric changes were observed in the small intestines of 11 neonatal gnotobiotic dogs after oral inoculation with a canine rotavirus. starting in pups killed at postinoculation hour (pih) 24, gross changes consisted of moderate dilatation and thinning of the walls of the small intestine, hyperemia, and a moderate amount of semiliquid-to-liquid greenish-yellow intestinal contents. in the jejunum and ileum of inoculated pups killed from pih 18 to 48, columnar vil ... | 1983 | 6625323 |
| analysis of gene selection in reassortant formation between canine rotavirus k9 and human rotaviruses with different antigenic specificities. | a number of antigenic mosaic reassortants which have neutralization proteins vp4 and vp7 derived from different parental strains were analysed in order to study gene selection in reassortant formation between animal and human rotaviruses (hrv). these reassortants were isolated from mixed infection of ma-104 cells with canine rotavirus strain k9 (subgroup i and g serotype 3) and hrv strains (with subgroup i or ii antigen and g serotype 1-4, 9 or 12 antigen), through repeated selections with anti- ... | 1993 | 7506839 |
| species specificity and interspecies relatedness in vp4 genotypes demonstrated by vp4 sequence analysis of equine, feline, and canine rotavirus strains. | we determined the nucleotide and deduced amino acid sequences of the vp4 genes of five equine, two feline, and two canine rotavirus strains. a high degree of homology (> 97.0%) was found among the vp4 amino acid sequences of the equine strains h2, fi-14, and fi23. equine strain l338 has a distinct vp4 amino acid sequence from those of the other equine strains (78.1% or less homology), and the l338 vp4 exhibited more than 17.0% divergence at the amino acid level from those of rotavirus strains pu ... | 1994 | 8178429 |
| a new serotype of the outer capsid protein vp4 shared by an unusual human rotavirus strain ro1845 and canine rotaviruses. | the vp4 protein of human rotavirus (hrv) strain ro1845 and canine rotavirus strains k9 and cu-1 exhibited greater than 98% amino acid identity within their group, but showed less identity with vp4 proteins of other hrv and animal rotavirus strains, the simian rotavirus strain rrv vp4 being most similar to them (90% amino acid identity). to exclude the possibility that these three strains were members of the rrv vp4 serotype p3, neutralization studies were performed using antisera to reassortant ... | 1993 | 8277285 |
| serotypic characterization of outer capsid spike protein vp4 of vervet monkey rotavirus sa11 strain. | the vervet monkey rotavirus sa11, a prototype strain of group a rotaviruses, has been shown to possess vp7 serotype 3 specificity but its neutralization specificity with regard to the other outer capsid protein vp4 has not been elucidated. we thus determined its vp4 specificity by two-way cross-neutralization with guinea pig antiserum prepared with a single gene substitution reassortant that had only the vp4-encoding gene from the simian rotavirus sa11 strain and remaining ten genes from human r ... | 1998 | 9687880 |
| functional analysis of the heterologous nsp1 genes in the genetic background of simian rotavirus sa11. | function of rotavirus nsp1 was analyzed by using single-nsp1 gene-substitution reassortants, skf, sdf, and snf which have the nsp1 gene derived from human rotaviruses ku, ds-1, and canine rotavirus k9, respectively, in the genetic background of simian rotavirus sa11. the nsp1 genes from ku, ds-1, k9, and sa11 exhibited 58-76% nucleotide sequence identity to one another. no substantial difference in viral growth was observed among the reassortants and sa11. however, production of nsp1 was not det ... | 1999 | 10481750 |
| development, characterization, and diagnostic applications of monoclonal antibodies against bovine rotavirus. | hybridomas secreting monoclonal antibodies (mabs) against the nebraska calf diarrhea strain of bovine rotavirus (brv) were characterized. indirect fluorescent-antibody assay, immunodot assay, and immunoprecipitation were used to select hybridomas that produced anti-brv mabs. seven of the mabs were shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot assay to be reactive with the brv outer capsid protein, vp7, which has a molecular mass of 37.5 kda. none of the seve ... | 2000 | 10702506 |
| human rotavirus hcr3 possesses a genomic rna constellation indistinguishable from that of feline and canine rotaviruses. | infection and spread of group a rotaviruses under natural conditions are mostly limited to one animal host species. however, rare molecular evidence exists for interspecies transmission by whole virions of animal rotaviruses to humans. human rotavirus strain hcr3, which was isolated in 1984 from a healthy infant in philadelphia, u.s.a. was shown by rna-rna hybridization to form 11 hybrid bands with feline rotavirus strain frv64 and canine rotavirus strains cu-1 and k9, but not with rotaviruses c ... | 2000 | 11205126 |
| nucleotide sequence variation of the vp7 gene of two g3-type rotaviruses isolated from dogs. | the sequence of the vp7 gene of two rotaviruses isolated from dogs in southern italy was determined and the inferred amino acid sequence was compared with that of other rotavirus strains. there was very high nucleotide and amino acid identity between canine strain rv198/95 and other canine strains, and to the human strain hcr3a. strain rv52/96, however, was found to have about 95% identity to the g3 serotype canine strains k9, a79-10 and cu-1 and 96% identity to strain rv198/95 and to the simian ... | 2001 | 11226570 |
| recent epidemiological status of canine viral enteric infections and giardia infection in japan. | epidemiology of canine enteric infections was studied. rectal swabs collected from 95 dogs presented at animal hospitals during a period from january to june of 2000 were examined for enteric pathogens, including viruses and giardia lamblia (g. lamblia). most frequently detected in both diarrheal and normal feces were canine coronavirus (55.4%) and g. lamblia (48.2%). canine parvovirus type 2 (cpv-2) was specifically associated with diarrheal cases and cpv-2b was the predominant antigenic type. ... | 2001 | 11411507 |
| [isolation of canine rotavirus and study of its immunobiological properties]. | canine rotavirus was isolated in ma104 roller culture of rhesus macaque cells. two passages in gnotobiotic puppies and two in colostrum-free puppies resulted in isolation of strain p of canine rotavirus. after 20 passages in ma104 culture the virus was adapted to mdck culture. optimal conditions for accumulation of canine rotavirus and its antigen (9.01 g tcd50/ml) in mdck culture are trypsin pretreatment of the virus inoculate in the final concentration of 50 mcg/ml for 30 min at 37 degrees c, ... | 2001 | 11449799 |
| isolation and genetic characterization of two g3p5a[3] canine rotavirus strains in italy. | two strains of canine rotavirus were isolated from pups with clinical signs of gastroenteritis. both strains were identified by polymerase chain reaction (pcr) as g3p5a[3], although restriction endonuclease analysis of the pcr amplicons revealed a genetic difference between the two isolates in the vp7 gene. the isolation in italy of canine rotaviruses displaying the same vp7 and vp4 specificities as in the usa and in japan, suggests that the g3 and p5a[3] types are highly conserved among canine ... | 2001 | 11516488 |
| isolation, serologic and molecular characterization of the first g3 caprine rotavirus. | we isolated a rotavirus in cell culture, named the grv strain, from a stool specimen of a korean goat with diarrhea, and performed an in-depth characterization. at various passage levels in cell culture, the grv strain retained its pathogenicity for goat kids, thereby for the first time establishing that a caprine rotavirus can cause diarrhea in goat kids. the grv strain grew to a high titer and agglutinated group o human erythrocytes. the grv vp7 protein was 96% identical with the rrv (simian r ... | 2003 | 12664291 |
| genetic characterization of canine rotavirus isolated from a puppy in korea and experimental reproduction of disease. | canine rotavirus was isolated from feces of a korean jindo dog with mild diarrhea, and the isolate was genetically characterized. rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. the virus isolate (gc/ks05) was identified as subtype g3p[3] using reverse transcription polymerase chain reaction (rt-pcr). the strain displayed 98% and 90% identity with the vp7 genes of a canin ... | 2007 | 17459836 |
| multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group a rotavirus strains. | rna-rna hybridization assays and complete genome sequence analyses have shown that feline rotavirus (frv) and canine rotavirus (crv) strains display at least two distinct genotype constellations (genogroups), represented by the frv strain rva/cat-tc/aus/cat97/1984/g3p[3] and the human rotavirus (hrv) strain rva/human-tc/jpn/au-1/1982/g3p3[9], respectively. g3p[3] and g3p[9] strains have been detected sporadically in humans. the complete genomes of two crv strains (rva/dog-tc/ita/rv198-95/1995/g3 ... | 2011 | 21609783 |
| canine rotavirus c strain detected in hungary shows marked genotype diversity. | species c rotaviruses (rvc) have been identified in humans and animals, including pigs, cows and ferrets. in dogs, rvc strains have been reported anecdotally on the basis of visualization of rotavirus-like virions by electron microscopy combined with specific electrophoretic migration patterns of the genomic rna segments. however, no further molecular characterization of these viruses was performed. here, we report the detection of a canine rvc in the stool of a dog with enteritis. analysis of t ... | 2015 | 26297005 |
| full-genome sequencing of a hungarian canine g3p[3] rotavirus a strain reveals high genetic relatedness with a historic italian human strain. | a canine rotavirus a strain was identified in the fecal specimen of a young dog during 2012 in hungary. the strain rva/dog-wt/hun/135/2012/g3p[3] shared complete genotype constellation (g3-p[3]-i3-r3-c3-m3-a15-n2-t3-e3-h6) and high genome sequence similarity (nt, 98.8 %) with a historic human strain, rva/human-tc/ita/pa260-97/1997/g3p[3]. this study provides evidence for the canine origin of the unusual nsp1 genotype, a15, and reinforces the hypothesis of direct interspecies transmission of cani ... | 2015 | 25634124 |
| whole genotype constellation of prototype feline rotavirus strains frv-1 and frv64 and their phylogenetic relationships with feline-like human rotavirus strains. | feline rotaviruses, members of the species rotavirus a, are an infrequent source of zoonotic infections, and were previously shown by rna-rna hybridization assays to possess two distinct genomic rna constellations, represented by strains frv-1 and frv64. due to the lack of whole genome sequence information for frv-1, human rotavirus strain au-1 has been used as a surrogate for the genotype constellation of feline rotaviruses. the aim of this study was to determine the whole genome sequence of fr ... | 2015 | 25351516 |
| rare g3p[3] rotavirus strain detected in brazil: possible human-canine interspecies transmission. | an unusual strain of human rotavirus g3p[3] (r2638 strain) was detected from a 1-year-old child patient during the epidemiological survey of rotavirus in the state of são paulo, brazil in 2011. | 2012 | 22398035 |
| identification of co-infection by rotavirus and parvovirus in dogs with gastroenteritis in mexico. | this is the first report on circulating canine rotavirus in mexico. fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe. | 2017 | 28716388 |