Publications

TitleAbstractYear
Filter
PMID
Filter
new porcine rotavirus serotype antigenically related to human rotavirus serotype 3.serotyping of porcine rotaviruses isolated in ma104 cells from australian piglets with diarrhea showed that two strains belonged to serotype 3 and one strain was antigenically similar to the osu strain of porcine rotavirus (serotype 5). in addition, neutralizing antibodies to human rotavirus serotype 4 (st-3 strain) were detected in serum samples from sows in one area, and so it seems probable that porcine rotaviruses of at least three serotypes occur in australia.19882830302
molecular characterization in the vp7, vp4 and nsp4 genes of human rotavirus serotype 4 (g4) isolated in japan and kenya.the vp7, vp4 and nsp4 genes of human rotavirus serotype 4 (g4) were analyzed to investigate intraserotypic variations. the techniques used included reverse transcription polymerase chain reaction with subtype specific primers, restriction fragment length polymorphism analysis and sequence analysis. twelve isolates (nine from japan and three from kenya) and two standard strains (hochi, odelia) were g4a p[8] wa group nsp4. a standard strain (st3) was g4a p[6] wa group nsp4 and a strain (va70) was ...200111293484
reactivity of vp4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses.thirteen hybridomas secreting vp4-specific monoclonal antibodies against the gottfried strain of porcine rotavirus (serotype 4) were produced and characterized. nine of the hybridomas secreted neutralizing monoclonal antibodies (n-mabs) against gottfried rotavirus. these n-mabs were divided into five distinct groups (groups i to v) according to their patterns of reactivity with different serotypes of human and animal rotaviruses. group i n-mabs (n = 3) were cross-reactive with five different ser ...19892556431
conservation in rotaviruses of the protein region containing the two sites associated with trypsin enhancement of infectivity.the primary structure of the region in the outer layer protein vp3, containing the two sites associated with trypsin enhancement of infectivity of rotavirus was found to be greatly conserved in cultivable human rotavirus serotypes 1 (wa), 2 (ds1), and 3 (p) and in four human rotaviruses directly purified from feces. significant differences with this conserved sequence were found in human rotavirus serotype 4 (st3), isolated from an asymptomatic neonate, and in seven animal rotaviruses. however, ...19863019004
identification of two subtypes of serotype 4 human rotavirus by using vp7-specific neutralizing monoclonal antibodies.two distinct subtypes of human rotavirus serotype 4 were identified by using neutralizing monoclonal antibodies directed to the major outer capsid glycoprotein, vp7, of strains st3 (subtype 4a) and va70 (subtype 4b). specimens containing serotype 4 rotavirus, obtained from different countries, were examined for subtyping by using solid-phase immune electron microscopy, enzyme-linked immunosorbent assay, and, for cell culture-adapted strains, neutralization assay. all 59 human rotavirus strains i ...19882842373
evidence of rotavirus intragenic recombination between two sublineages of the same genotype.rotavirus g4 prevalence increased during the past decade, with one of the highest prevalences reported during rotavirus surveillance in argentina. intragenotype diversity analysis has led to its subdivision into lineages (i and ii) and sublineages (ia-id). on analysis of argentine and g4 vp7 sequences from other locations, one argentine strain (argres1723) appeared to be an intermediate between g4 sublineages ib and ic. similarity and bootscanning analyses and sawyer's test were carried out to d ...200415166456
[expression of vp7 from a group a rotavirus g4 field strain in baculovirus system].the full length of g4 type vp7 cdna was amplified by reverse transcription and polymerase chain reaction (rt-pcr) from a group a rotavirus field strain cr117 circulated in beijing. the amplified cdna was first cloned into ptz18. sequence analysis showed 96.9% homology of the deduced amino acid between vp7 of cr117 and st3. then the vp7 cdna of cr117 was subcloned into the transfer vector pbacpak8 downstream of the polyhedrin promotor. insect cells were cotransfected with recombinant plasmid pbac ...199912555424
characterization of the neutralizing epitopes of vp7 of the gottfried strain of porcine rotavirus.the neutralization epitopes of the outer capsid protein vp7 of a porcine group a rotavirus were studied by using neutralizing monoclonal antibodies (n-mabs). six n-mabs which were specific for the vp7 protein of the gottfried strain of porcine rotavirus (serotype g4) were used for analyzing the antigenic sites of vp7. three different approaches were used for this analysis: testing the serological reactivity of each n-mab against different g serotypes of human and animal rotaviruses, analyzing n- ...19937691871
sequence conservation within neutralization epitope regions of vp7 and vp4 proteins of human serotype g4 rotavirus isolates.serotype g4 rotavirus isolates causing four separate epidemics of severe diarrhoea in young children in melbourne, australia (from 1974-1990) were investigated for sequence variation in genes encoding the outer capsid proteins, vp4 and vp7. complementary dna of the gene encoding the major outer capsid neutralization antigen, vp7, of eighteen isolates was synthesized and amplified by coupled reverse transcription and polymerase chain reaction. direct sequencing methods were used to derive the ded ...19937504915
single gene substitution rotavirus reassortants containing the major neutralization protein (vp7) of human rotavirus serotype 4.a series of reassortants was isolated from coinfection of cell cultures with wild-type bovine rotavirus (uk strain [serotype 6]) or rhesus rotavirus (strain mmu18006 [serotype 3]) and a tissue culture-adapted human rotavirus strain, st3 (serotype 4). monospecific antiserum or a set of monoclonal antibodies to the major outer capsid neutralization glycoprotein, vp7, of the animal rotavirus parent was used to select for reassortants with human rotavirus serotype 4 neutralization specificity. the m ...19863021814
Displaying items 1 - 10 of 10