| screening of the closterovirus genome by degenerate primer-mediated polymerase chain reaction. | the genome of beet yellows virus (byv), the type representative of the closterovirus group, encodes a homologue of the cellular heat-shock protein (hsp) 70 family. a pair of degenerate primers targeted to motifs a and e, which are highly conserved in hsp70s, was synthesized. genomes of several definite and possible members of the closterovirus group were screened for the presence of the hsp70 gene with pcr using these degenerate primers. byv, citrus tristeza virus (ctv), beet yellow stunt virus ... | 1994 | 8207405 |
| organization of the 3'-terminal half of beet yellow stunt virus genome and implications for the evolution of closteroviruses. | the 3'-terminal half of the beet yellow stunt virus (bysv) genome 10,545 nt, has been cloned and sequenced. the sequenced portion of the bysv genome encompasses 10 open reading frames (orfs) and 241 nt of the 3' untranslated region. the sequence spans, in the 5' to 3' direction, the c-terminal region of the replication-associated polyprotein gene (orf 1a) which includes the set of motifs typical of helicases (hel), the entire 53-kda polymerase (rdrp) gene (orf 1b), and genes encoding 30-kda (orf ... | 1996 | 8661428 |
| nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 are similar to beet yellows virus, the closterovirus type member. | the entire genome of grapevine leafroll-associated closterovirus-2 (glrav-2), except the exact 5' terminus, was cloned and sequenced. the sequence encompasses nine open reading frames (orfs) which include, in the 5' to 3' direction, an incomplete orf1a encoding a putative viral polyprotein and eight orfs that encode proteins of 52 kda (orf1b), 6 kda (orf2), 65 kda (orf3), 63 kda (orf4), 25 kda (orf5), 22 kda (orf6), 19 kda (orf7) and 24 kda (orf8) respectively, and 216 nucleotides of the 3' untr ... | 1998 | 9603345 |
| characterization of the beet yellow stunt virus coat protein gene. | abstract the beet yellow stunt virus (bysv) genome contains at least nine open reading frames (orfs) that code for proteins ranging from 6 to 66 kda. based on amino acid sequence comparisons, the coat protein (cp) was previously identified as the product of orf7. we expressed the product of orf7 in bacteria and confirmed that orf7 codes for the bysv cp by immunoblotting. bysv is a phloem-limited virus, and virus cp antigen of a quality sufficient for diagnostic antisera production has not been a ... | 1998 | 18944815 |
| molecular characterization of a closterovirus from carrot and its identification as a german isolate of carrot yellow leaf virus. | a high-molecular-weight dsrna (approximately 15 kbp) was isolated from chlorotic leaves of a carrot plant and used for determining the entire nucleotide sequence of a closterovirus. the complete genome of this carrot closterovirus (ccv) was 16.4 kb in length and contained ten open reading frames (orfs). the genome organization of ccv resembled that of beet yellow stunt virus, but orf2 and orf3 were in a reversed order. based on hsp70h sequences, ccv is most closely related to carnation necrotic ... | 2009 | 19575278 |
| biological, molecular, and serological studies of a novel strain of grapevine leafroll-associated virus 2. | in california, a novel closterovirus was detected in "redglobe" grapevine, associated with graft incompatibility and given a trivial name "grapevine rootstock stem lesion associated virus (grslav)." the biological properties of the putative virus were ascertained when asymptomatic yet infected redglobe scion buds were graft-inoculated onto test plants of cabernet sauvignon propagated on 18 different rootstocks. it proved lethal on test plants growing on rootstocks 1616c, 5bb, 5c, 3309c, and 1103 ... | 2011 | 21487783 |
| beet yellow stunt virus in the phloem of sonchus oleraceus l. | | 1981 | 6268801 |
| beet yellow stunt virus in cells of sonchus oleraceus l. and its relation to host mitochondria. | in sonchus oleraceus l. (asteraceae) infected with the beet yellow stunt virus (bysv) the virions are found in phloem cells, including the sieve elements. in parenchymatous phloem cells, the virus is present mainly in the cytoplasm. in some parenchymatous cells, containing massive accumulations of virus, the flexuous rodlike virus particles are found partly inserted into mitochondrial cristae. the mitochondrial envelope is absent where virus is present in the cristae. a similar relation between ... | 1979 | 18631608 |
| annulate lamellae in phloem cells of virus-infected sonchus plants. | the occurrence of annulate lamellae (al) in differentiating phloem of sonchus oleraceus (compositae) singly infected with sowthistle yellow vein virus (syvv) and doubly infected with a combination of syvv and beet yellow stunt virus is documented by electron microscopy. cell types in which al were found were immature sieve elements and phloem parenchyma cells. al were found only in cells that also contained syvv particles although a direct association between the virus and al was not apparent. t ... | 1977 | 873998 |
| electron microscopy of beta leaves infected with beet yellow stunt virus. | | 1970 | 4099082 |