| sequence analysis of the 3'-terminal half of rna 1 of wheat spindle streak mosaic virus. | cdna complementary to the 3'-terminal half of rna 1 of wheat spindle streak mosaic virus (wssmv) from southern france has been cloned and sequenced. one large open reading frame (orf) of 4410 nucleotides and a nontranslated region (ntr) of 213 nucleotides at the 3'-end excluding the poly(a)-tail were found. because of the amino acid sequence homology to the polyprotein of barley yellow mosaic virus (baymv) rna 1, the encoded polyprotein of the sequenced region of wssmv is supposed to comprise th ... | 1994 | 7979968 |
| structural proteins of three viruses in the potyviridae adhere only to their homologous cylindrical inclusions in mixed infections. | immunoelectron microscopy of serial sections of leaf cells of wheat doubly infected by wheat streak mosaic virus and agropyron mosaic virus or wheat spindle streak mosaic virus showed that structural proteins or virions associated only with homologous cylindrical inclusions (ci) or with self in aggregates. ci protein of wsmv was seen firmly attached to, and extended through, a plasmodesma. based on ci protein similarities with transport protein families, membrane-associated secretion proteins, o ... | 1993 | 8373700 |
| analysis of nucleotide sequence of wheat yellow mosaic virus genomic rnas. | wheat yellow mosaic virus (wymv) isolate hc was used for viral cdna synthesis and sequencing. the results show that the viral rnal is 7 629 nucleotides encoding a polyprotein with 2 407 amino acids, from which seven putative proteins may be produced by an autolytic cleavage processing besides the viral coat protein. the rna2 is 3 639 nucleotides and codes for a polyprotein of 903 amino acids, which may contain two putative non-structural proteins. although wymv shares a similarity in genetic org ... | 1999 | 18726520 |
| soil-borne viruses affecting cereals: known for long but still a threat. | a number of furo- and bymoviruses, which are naturally transmitted by the ubiquitous soil-borne protozoon polymyxa graminis are of great economic importance for autumn-sown wheat, barley, rye, triticale, and oats. the two barley-affecting bymoviruses, barley yellow mosaic virus and barley mild mosaic virus, are widespread in europe and east asia. production of wheat, rye, and triticale is threatened by three related furoviruses and two bymoviruses. soil-borne wheat mosaic virus mainly occurs in ... | 2009 | 19159654 |
| broad-spectrum detection and quantitation methods of soil-borne cereal mosaic virus isolates. | a broad-spectrum reverse transcription-polymerase chain reaction (rt-pcr) protocol was developed for detecting soil-borne cereal mosaic virus (sbcmv) isolates, responsible for mosaic diseases in europe, using primers targeting the highly conserved 3'-untranslated region of rna-1 and rna-2 of sbcmv. the 3'-end region is a privileged target for the detection of a wide range of isolates, because of sequence conservation, of the trna-like structure, the major role in viral replication and the signal ... | 2009 | 19490978 |
| endoplasmic reticulum-derived pinwheels in wheat infected with wheat spindle streak mosaic virus. | | 2015 | 4728828 |
| occurrence of viruses and associated grain yields of paired symptomatic and nonsymptomatic tillers in kansas winter wheat fields. | vector-borne virus diseases of wheat are recurrent in nature and pose significant threats to crop production worldwide. in the spring of 2011 and 2012, a state-wide sampling survey of multiple commercial field sites and university-managed kansas agricultural experiment station variety performance trial locations spanning all nine crop-reporting regions of the state was conducted to determine the occurrence of barley yellow dwarf virus-pav (bydv-pav), cereal yellow dwarf virus-rpv, wheat streak m ... | 2016 | 26799958 |
| using air pressure cells to evaluate the effect of soil environment on the transmission of soilborne viruses of wheat. | abstract an air pressure cell, a laboratory tool that precisely controls soil matric potential, was utilized in a novel approach to investigate the epidemiology and management of soilborne disease. matric potentials of -1, -5, -20, and -40 kpa were established in cores of field soil infested with wheat soilborne mosaic virus (wsbmv) and its presumed vector polymyxa graminis. equilibrated soil cores were planted to wheat (triticum aestivum), and after intervals of growth under controlled environm ... | 2003 | 18944097 |
| the effects of postplanting environment on the incidence of soilborne viral diseases in winter cereals. | abstract soilborne wheat mosaic virus (sbwmv) and wheat spindle streak mosaic virus (wssmv) are putatively transmitted to small grains by the obligate parasite polymyxa graminis, but little is known about environmental requirements for transmission and the resulting disease incidence. we planted susceptible wheat and triticale cultivars in field nurseries on different autumn dates in 3 years and observed the incidence of symptomatic plants in each following spring. autumn postplanting environmen ... | 2004 | 18943773 |
| soilborne wheat mosaic virus movement protein and rna and wheat spindle streak mosaic virus coat protein accumulate inside resting spores of their vector, polymyxa graminis. | to study virus-vector interactions between soilborne wheat mosaic virus (sbwmv) or wheat spindle streak mosaic virus (wssmv) and polymyxa graminis ledingham, p. graminis was propagated in plants grown hydroponically. p. graminis accumulated to high levels in several barley cultivars tested. multiple developmental stages of p. graminis could be identified in infected barley roots. accumulation of sbwmv and wssmv inside p. graminis sporosori in the roots of soil-grown winter wheat and hydroponical ... | 2004 | 15242168 |
| induction of 4vs chromosome recombinants using the cs ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from haynaldia villosa. | the wheat spindle streak mosaic virus (wssmv) or wheat yellow mosaic virus (wymv) resistance gene, wss1, from haynaldia villosa, was previously mapped to the chromosome arm 4vs by the development of 4v (4d) substitution and t4dl·4vs translocation lines. for better utilization and more accurate mapping of the wss1, in this research, the cs ph1b mutant was used to induce new translocations with shortened 4vs chromosome fragments. thirty-five homozygous translocations with different alien fragment ... | 2013 | 23989649 |
| epistatic determinism of durum wheat resistance to the wheat spindle streak mosaic virus. | the resistance of durum wheat to the wheat spindle streak mosaic virus (wssmv) is controlled by two main qtls on chromosomes 7a and 7b, with a huge epistatic effect. wheat spindle streak mosaic virus (wssmv) is a major disease of durum wheat in europe and north america. breeding wssmv-resistant cultivars is currently the only way to control the virus since no treatment is available. this paper reports studies of the inheritance of wssmv resistance using two related durum wheat populations obtain ... | 2017 | 28451771 |
| the complete nucleotide sequence of barley mild mosaic virus rna1 and its relationship with other members of the potyviridae. | the complete nucleotide sequence of rna1 of a french barley mild mosaic bymovirus isolate has been determined. the molecule is composed of 7261 nucleotides and contains a single large open reading frame encoding a protein of 2258 amino acids with a calculated mr of 256,375. amino acid sequence comparison with poty- and rymoviruses reveals eight domains corresponding to the potyviral p3, 6k1, cl, 6k2, nla-vpg, nla-pro, nlb and capsid proteins, respectively. seven putative cleavage sites, similar ... | 1996 | 8623538 |