| reactivity of recombinant and mutant vanadium bromoperoxidase from the red alga corallina officinalis. | vanadium bromoperoxidase (vbpo) from the marine red alga corallina officinalis has been cloned and heterologously expressed in esherichia coli. the sequence for the full-length cdna of vbpo from c. officinalis is reported. steady state kinetic analyses of monochlorodimedone bromination reveals the recombinant enzyme behaves similarly to native vbpo from the alga. the kinetic parameters (k(m)(br-)=1.2 mm, k(m)(h(2)o(2))=17.0 microm) at the optimal ph 6.5 for recombinant vbpo are similar to report ... | 2002 | 12121762 |
| dynamics of potentially protective compounds in rhodophyta species from patagonia (argentina) exposed to solar radiation. | the impact of solar radiation upon potentially protective compounds (i.e., uv-absorbing compounds and carotenoids) was assessed in four rhodophyte species from patagonia (i.e., ceramium sp. lyngbye, corallina officinalis linnaeus, callithamnion gaudichaudii agardh and porphyra columbina montagne) during short-term (i.e., 46 h) experiments. algae were exposed to solar radiation under two treatments (par only: 400-700 nm, and par+uvr: 280-700 nm) and sub-samples were taken every 3 h (or longer per ... | 2004 | 15246352 |
| effects of solar radiation on the patagonian rhodophyte corallina officinatis (l.). | experiments were conducted in patagonian waters (argentina) to assess the impact of solar radiation (par, 400-700 nm, and uvr, 280-400 nm) upon two strains of the red alga corallina officinalis linnaeus, characteristic of the mid and lower intertidal zone, during march 2000. fluorescence parameters were determined using a pulse amplitude modulated (pam) fluorometer. the two strains had different initial optimal quantum yields but similar strong decreases in the quantum yield when the algae were ... | 2003 | 16245043 |
| ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae corallina officinalis l. | the response of the antioxidant defense system of an intertidal macroalgae corallina officinalis l. to different dosages of uv-b irradiation was investigated. results showed that superoxide dimutase (sod) and peroxidase (pox) increased and then maintained at a relatively stable level when subjected to uv-b irradiation. catalase (cat) activity under medium dosage of uv-b irradiation (muv) and high dosage of uv-b irradiation (huv) treatments were significantly decreased. ascorbate peroxidase (apx) ... | 2010 | 20608508 |
| steroids and an oxylipin from an algicolous isolate of aspergillus flavus. | a new oxylipin, (8e,12z)-10,11-dihydroxyoctadeca-8,12-dienoic acid (1), a new steroid, 3ß,4a-dihydroxy-26-methoxyergosta-7,24(28)-dien-6-one (2), and four known steroids, episterol (3), (22e,24r)-ergosta-7,22-dien-3ß,5a,6a-triol (4), (22e,24r)-ergosta-5,22-dien-3ß-ol (5), and (22e,24r)-ergosta-4,6,8(14),22-tetraen-3-one (6), were isolated from the cultures of aspergillus flavus, an endophytic fungus isolated from the marine red alga corallina officinalis. their structures and relative stereochem ... | 2011 | 21452344 |
| bromoperoxidase of the macroalga corallina officinalis. | | 2006 | 1426568 |
| extracts of north sea macroalgae reveal specific activity patterns against attachment and proliferation of benthic diatoms: a laboratory study. | a variety of macroalgae (ceramium rubrum, corallina officinalis, palmaria palmata, mastocarpus stellatus, fucus vesiculosus, cladophora rupestris, ulva sp.) were investigated by scanning electron microscopy to visualize epiphytic colonizers. the macroalgae differed in terms of their epiphytic coverage of bacteria, fungi and diatoms. macroalgae, largely devoid of epiphytic diatoms, were hypothesized to employ effective antifouling means to reduce epiphytic coverage, whilst heavily fouled macroalg ... | 2008 | 18092216 |
| assessing the use of the mitochondrial cox1 marker for use in dna barcoding of red algae (rhodophyta). | the red algae, a remarkably diverse group of organisms, are difficult to identify using morphology alone. following the proposal to use the mitochondrial cytochrome c oxidase subunit i (cox1) for dna barcoding animals, we assessed the use of this gene in the identification of red algae using 48 samples plus 31 sequences obtained from genbank. the data set spanned six orders of red algae: the bangiales, ceramiales, corallinales, gigartinales, gracilariales and rhodymeniales. the results indicated ... | 2006 | 21642175 |
| a card-fish protocol for the identification and enumeration of epiphytic bacteria on marine algae. | a card-fish protocol was developed and applied to analyse surface-associated bacteria on the marine algae ulva lactuca, delisea pulchra, corallina officinalis, amphiroa anceps, porphyra sp. and sargassum linearifolium. the combination of alexa(546)-labelled tyramide as the reporter molecule with sybr green ii counterstain allowed for superior detection of the hybridised probe fluorescence against plant tissue from which pigment autofluorescence has been reduced. | 2006 | 16216355 |
| crystal structure of dodecameric vanadium-dependent bromoperoxidase from the red algae corallina officinalis. | the three-dimensional structure of the vanadium bromoperoxidase protein from the marine red macroalgae corallina officinalis has been determined by single isomorphous replacement at 2.3 a resolution. the enzyme subunit is made up of 595 amino acid residues folded into a single alpha+beta domain. there are 12 bromoperoxidase subunits, arranged with 23-point group symmetry. a cavity is formed by the n terminus of each subunit in the centre of the dodecamer. the subunit fold and dimer organisation ... | 2000 | 10843856 |
| on the regiospecificity of vanadium bromoperoxidase. | vanadium haloperoxidase enzymes catalyze the oxidation of halide ions by hydrogen peroxide, producing an oxidized intermediate, which can halogenate an organic substrate or react with a second equivalent of hydrogen peroxide to produce dioxygen. haloperoxidases are thought to be involved in the biogenesis of halogenated natural products isolated from marine organisms, including indoles and terpenes, of which many are selectively oxidized or halogenated. little has been shown concerning the abili ... | 2001 | 11457064 |
| purification, crystallisation and preliminary x-ray analysis of the vanadium-dependent haloperoxidase from corallina officinalis. | the vanadium-dependent haloperoxidase from the seaweed corallina officinalis has been purified to homogeneity and crystallised. the protein is reported to be a hexamer of 12 x 64,000 da, contains no haem, and is dependent on vanadium for activity. the crystals are grown from polyethylene glycol (peg) 6,000 and 0.4 m potassium chloride. they are stable and diffract to better than 2 a resolution. they are of a cubic space group i23 (or 12(1)3) with cell dimensions a = b = c = 310 a. | 1995 | 7867808 |
| structure of the 'corallinans'--sulfated xylogalactans from corallina officinalis. | the structure of the main polysaccharides extracted from the red seaweed corallina officinalis was characterized by methylation, desulfation-methylation, and ethylation analysis. the backbone has an alternating-->4)-alpha-l-gal-(1-->3)-beta-d-gal-(1-->structure. the c6 position of 3-linked units is substituted mainly by beta-d-xylosyl side stubs but also by sulfate ester groups and minor amounts of 4-o-methylgalactosyl side stubs. positions c2 and c3 of 4-linked units also carry sulfate or metho ... | 1994 | 8011594 |
| thermostability of the vanadium bromoperoxidase from corallina officinalis. | | 1993 | 8132014 |
| vanadium haloperoxidase-catalyzed bromination and cyclization of terpenes. | marine red algae (rhodophyta) are a rich source of bioactive halogenated natural products, including cyclic terpenes. the biogenesis of certain cyclic halogenated marine natural products is thought to involve marine haloperoxidase enzymes. evidence is presented that vanadium bromoperoxidase (v-brpo) isolated and cloned from marine red algae that produce halogenated compounds (e.g., plocamium cartilagineum, laurencia pacifica, corallina officinalis) can catalyze the bromination and cyclization of ... | 2003 | 12656585 |
| zinc concentrations in marine macroalgae and a lichen from western ireland in relation to phylogenetic grouping, habitat and morphology. | zinc concentrations in 19 species of marine macroalgae and a lichen from western ireland (spiddal, co. galway) were analysed by atomic absorption spectrophotometry. algae were collected from a single site but occupied different shore levels and belonged to distinct phylogenetic groupings and to different (previously recognised) morphological groups. concentrations ranged from 15-115 microg g(-1) dry weight. the greatest variation in concentration occurred amongst the red algal species, containin ... | 2004 | 15111037 |
| complexity and idiosyncrasy in the responses of algae to disturbance in mono- and multi-species assemblages. | there is considerable debate about whether stability (e.g. inertia) of an assemblage, or of individuals in an assemblage, is positively associated with the number of species or whether there are idiosyncratic effects of particular species. we assessed the general model that the loss of an individual alga, caused by trampling, is greater in monospecific than in multi-species stands but that the responses of algae are idiosyncratic, depending on the morphology of the species. the experiment was do ... | 2008 | 18574597 |
| biodiversity of coralline algae in the northeastern atlantic including corallina caespitosa sp. nov. (corallinoideae, rhodophyta)(1). | the corallinoideae (corallinaceae) is represented in the northeastern atlantic by corallina officinalis l.; corallina elongata j. ellis et sol.; haliptilon squamatum (l.) h. w. johans., l. m. irvine et a. m. webster; and jania rubens (l.) j. v. lamour. the delimitation of these geniculate coralline red algae is based primarily on morphological characters. molecular analysis based on cox1 and 18s rrna gene phylogenies supported the division of the corallinoideae into the tribes janieae and corall ... | 2009 | 27033664 |
| efficient purification with high recovery of vanadium bromoperoxidase from corallina officinalis. | a novel, simple and highly efficient process for purifying vanadium bromoperoxidase from corallina officinalis is reported. the key innovation is adding 0.5 mm sodium orthovanadate to the crude cell extract followed by heating at 70°c for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. combining the heat treatment with ammonium sulfate precipitation and deae-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously ... | 2011 | 21046195 |
| in vitro antioxidant activities of sulfated polysaccharide fractions extracted from corallina officinalis. | sulfated polysaccharides (f1, f2) from seaweed corallina officinalis were isolated through anion-exchange column chromatography. their chemical characteristics were determined by gc, hplc, ft-ir and uv spectra. f1 and f2 contained only two monosaccharides, namely galactose and xylose. the antioxidant activities of f1, f2 and the de-sulfated polysaccharides (df-1, df-2) in vitro were investigated, including hydroxyl radicals scavenging effect, superoxide radical scavenging capacity, dpph radical ... | 2011 | 21896282 |
| a study of the relationship between process conditions and mechanical strength of mineralized red algae in the preparation of a marine-derived bone void filler. | bone void fillers that can enhance biological function to augment skeletal repair have significant therapeutic potential in bone replacement surgery. this work focuses on the development of a unique microporous (0.5-10 microm) marine-derived calcium phosphate bioceramic granule. it was prepared from corallina officinalis, a mineralized red alga, using a novel manufacturing process. this involved thermal processing, followed by a low pressure-temperature chemical synthesis reaction. the study fou ... | 2011 | 22034740 |
| do cues matter? highly inductive settlement cues don't ensure high post-settlement survival in sea urchin aquaculture. | increasing settlement and post-settlement survival during the critical transition from planktonic larvae to benthic juveniles will increase efficiency for sea urchin aquaculture. this study investigated the effects of temperature and settlement cues on the settlement and post-settlement survival of the sea urchin tripneustes gratilla during this phase. the current commercial methodology, which utilises natural biofilm settlement plates, was tested and resulted in low settlement (<2%) and poor po ... | 2011 | 22162755 |
| chiharaea and yamadaia (corallinales, rhodophyta) represent reduced and recently derived articulated coralline morphologies(1). | phycologists have hypothesized that the diminutive fronds produced by species in the genera chiharaea and yamadaia, which are composed of comparatively few genicula and intergenicula, represent morphological intermediates in the evolution of articulated corallines from crustose ancestors. we test this "intermediate frond hypothesis" by comparing rbcl sequences from the generitype species chiharaea bodegensis and yamadaia melobesioides to sequences from other coralline genera. we demonstrate that ... | 2012 | 27008997 |
| elevated co2 levels affect the activity of nitrate reductase and carbonic anhydrase in the calcifying rhodophyte corallina officinalis. | the concentration of co(2) in global surface ocean waters is increasing due to rising atmospheric co(2) emissions, resulting in lower ph and a lower saturation state of carbonate ions. such changes in seawater chemistry are expected to impact calcification in calcifying marine organisms. however, other physiological processes related to calcification might also be affected, including enzyme activity. in a mesocosm experiment, macroalgal communities were exposed to three co(2) concentrations (380 ... | 2013 | 23314813 |
| habitat-associations of turban snails on intertidal and subtidal rocky reefs. | patchiness of habitat has important influences on distributions and abundances of organisms. given the increasing threat of loss and alteration of habitats due to pressures associated with humans, there is a need for ecologists to understand species' requirements for habitat and to predict changes to taxa under various future environmental conditions. this study tested hypotheses about the generality of patterns described for one species of marine intertidal turban snail for a different, yet clo ... | 2013 | 23675409 |
| misleading morphologies and the importance of sequencing type specimens for resolving coralline taxonomy (corallinales, rhodophyta): pachyarthron cretaceum is corallina officinalis. | coralline red algae play a key role in the ecology of near shore marine ecosystems and are increasingly being used to study the effects of climate change in the marine environment. corallines are very difficult to identify to species, and even to genus, using morpho-anatomy, likely complicating studies of their ecology, physiology, and biodiversity. we sequenced a 296 base pair fragment of chloroplast dna from a 187-year-old isolectotype specimen of pachyarthron cretaceum, a morphologically dist ... | 2014 | 26988460 |
| corallina and ellisolandia (corallinales, rhodophyta) photophysiology over daylight tidal emersion: interactions with irradiance, temperature and carbonate chemistry. | the photophysiology of three geniculate coralline algal species (corallina officinalis, c. caespitosa and ellisolandia elongata) was determined in intertidal rock pools in the south-west uk at combe martin (51°12'31n 4°2'19w) and heybrook bay (50°31'66n 4°11'41w), at the start, middle and end of summer (september 1 and 2) and winter (february 9 and 10) daylight tidal emersion periods, in relation to prevailing irradiance, temperature and carbonate chemistry conditions. algal photophysiology was ... | 2014 | 25170177 |
| production of hydroxylated polybrominated diphenyl ethers from bromophenols by bromoperoxidase-catalyzed dimerization. | hydroxylated polybrominated diphenyl ethers (ho-pbdes) are emerging endocrine-disrupting compounds that are widely present in the marine environment. the origin of ho-pbdes is generally attributed to metabolism of pbdes and natural production in the environment. however, it is unclear how ho-pbdes are produced naturally. here we report the formation of ho-pbdes from simple bromophenols (bps) [e.g., 2,4-dibromophenol (2,4-dbp) and 2,4,6-tribromophenol (2,4,6-tbp)] under the catalysis of bromopero ... | 2014 | 25229997 |
| microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging. | calcifying coralline macroalgae provide biogenic habitats colonised by epiphytic microalgae that contribute significantly to community productivity. georeferenced hyperspectral and high-resolution fluorescence imaging were coupled to microspatially mapped community composition and relative biomass of macroalgal host and epiphyte microalgal groups, and their weighted contributions to productivity within host fronds of corallina officinalis on upper and lower zones of a rocky shore were determined ... | 2016 | 26923719 |
| characterising the microbiome of corallina officinalis, a dominant calcified intertidal red alga. | the living prokaryotic microbiome of the calcified geniculate (articulated) red alga, corallina officinalis from the intertidal seashore is characterised for the first time based on the v6 hypervariable region of 16s rrna. results revealed an extraordinary diversity of bacteria associated with the microbiome. thirty-five prokaryotic phyla were recovered, of which proteobacteria, cyanobacteria, bacteroidetes, actinobacteria, planctomycetes, acidobacteria, verrucomicrobia, firmicutes and chlorofle ... | 2016 | 27222222 |
| arsenobetaine and thio-arsenic species in marine macroalgae and herbivorous animals: accumulated through trophic transfer or produced in situ? | arsenobetaine (ab) and thio-arsenoribosides were measured in common macroalgae species (8 phaeophyta, 4 rhodophyta and 2 chlorphyta), along the australian south east coast line. as well, arsenic species profiles were measured for two common marine herbivores, the sea urchin centrostephanus rodgersii and the fish odax cyanomelas that graze on these macroalgae to understand if trophic transfer of these species would account for their presence in marine herbivores. ab was found in seven of the four ... | 2016 | 28007168 |
| different speciation for bromine in brown and red algae, revealed by in vivo x-ray absorption spectroscopic studies. | members of various algal lineages are known to be strong producers of atmospherically relevant halogen emissions, that is a consequence of their capability to store and metabolize halogens. this study uses a noninvasive, synchrotron-based technique, x-ray absorption spectroscopy, for addressing in vivo bromine speciation in the brown algae ectocarpus siliculosus, ascophyllum nodosum, and fucus serratus, the red algae gracilaria dura, g. gracilis, chondrus crispus, osmundea pinnatifida, asparagop ... | 2014 | 26988449 |
| cytotoxic activity of biosynthesized gold nanoparticles with an extract of the red seaweed corallina officinalis on the mcf-7 human breast cancer cell line. | nano-biotechnology is recognized as offering revolutionary changes in the field of cancer therapy and biologically synthesized gold nanoparticles are known to have a wide range of medical applications. | 2014 | 24935390 |
| antiprotozoal, antimycobacterial and cytotoxic potential of twenty-three british and irish red algae. | as part of our continuing research on seaweeds, we have screened the crude extracts of 23 red marine algae collected from england and ireland. the clinically important blood-stage life forms of trypanosoma brucei rhodesiense, t. cruzi, leishmania donovani and mycobacterium tuberculosis were used as test organisms in the in vitro assays. the selectivity of the extracts was determined by using mammalian skeletal myoblast (l6) cells. all algal extracts showed activity against t. brucei rhodesiense, ... | 2010 | 20077438 |
| the dodecameric vanadium-dependent haloperoxidase from the marine algae corallina officinalis: cloning, expression, and refolding of the recombinant enzyme. | the dodecameric vanadium-dependent bromoperoxidase from corallina officinalis has been cloned and over-expressed in escherichia coli. however, the enzyme was found to be predominantly in the form of inclusion bodies. this protein presents a challenging target for refolding, both due to the size (768kda) and quaternary structure (12x64kda). successful refolding conditions have been established which result in an increase in the final yield of active bromoperoxidase from 0.5mg to 40mg per litre of ... | 2007 | 17049263 |
| seasonal variation of the lipoidal matters and hypolipidaemic activity of the red alga corallina officinalis l. | the lipoidal matters of corallina officinalis l. showed a seasonal nonsignificant quantitative variation. however, the fatty acids revealed a relative increase in the summer and winter, while unsaponifiable matter exhibited a slight increase in the spring. the gc/ms analysis of saponifiable and unsaponifiable matter of the algal samples collected in different seasons revealed that samples collected in the spring contained a low cholesterol content and high steroidal compounds as well as high pol ... | 2003 | 12557241 |
| asymmetric sulfoxidation catalyzed by a vanadium-containing bromoperoxidase. | a vanadium-containing bromoperoxidase (vbrpo) from the alga corallina officinalis has been shown to catalyze the stereoselective oxidation of some aromatic bicyclic sulfides to the corresponding (s)-sulfoxides in high (up to 91%) ee. hydrogen peroxide was found to have a large effect on the catalyzed reaction, most likely due to an inhibition of vbrpo. high optical and chemical yields were found to be favored by a continuous slow addition of hydrogen peroxide to keep a low excess. the reaction g ... | 1997 | 11671985 |
| preliminary x-ray analysis of a new crystal form of the vanadium-dependent bromoperoxidase from corallina officinalis. | a new crystal form of the vanadium-dependent bromoperoxidase from corallina officinalis has been obtained. the crystals exhibit a 'teardrop' morphology and are grown from 2 m ammonium dihydrogen phosphate ph and diffract to beyond 1.7 a resolution. they are in tetragonal space group p4222 with unit-cell dimensions of a = b = 201.9, c = 178.19 a, alpha = beta = gamma = 90 degrees. a 2.3 a resolution native data set has been collected at the hamburg synchrotron. a mercury derivative data set has a ... | 1998 | 9761927 |
| extraction of a calcification inhibitor from corallina officinalis. | | 1992 | 1633974 |
| vanadate activation of bromoperoxidase from corallina officinalis. | a nonheme bromoperoxidase has been purified to homogeneity from the red seaweed corallina officinalis. like the corresponding enzyme previously reported from c. pilulifera, this bromoperoxidase contains a significant amount of nonheme iron. however, it is vanadate ion and not iron that activates the enzyme, and maximal activity is achieved with stoichiometric vanadium incorporation. the absence of competition between vanadium and iron suggests that they occupy distinct binding sites in the prote ... | 1989 | 2540754 |
| [diagnosis and anthelminthic action of alsidium helminthocorton kützing (corsican moss), of jania rubens lamour and of corallina officinalis l]. | | 1983 | 6625480 |
| [amino acids of the corsican moss, alsidium helminthocroton kutzing, jania rubens lamx. and corallina officinalis l]. | | 1982 | 7185317 |