comparison of the host ranges and antigenicity of cryptosporidium parvum and cryptosporidium wrairi from guinea pigs.oocysts of a cryptosporidium isolate from guinea pigs were not infectious for adult mice, but were infectious for two of three newborn calves and for suckling mice. however, oocysts isolated from calves or mice infected with guinea pig cryptosporidium were not infectious for guinea pigs. four isolates of c. parvum from calves were incapable of infecting weanling guinea pigs. microscopic examination of tissue from the colon and cecum of suckling guinea pigs inoculated with c. parvum revealed spar ...19921386385
effect of hyperimmune bovine colostrum raised against cryptosporidium parvum on infection of guinea pigs by cryptosporidium wrairi.oocysts shedding was markedly reduced in guinea pigs inoculated intraintestinally with cryptosporidium wrairi sporozoites that had been incubated with hyperimmune bovine colostrum raised to c. parvum when compared with shedding in guinea pigs inoculated with sporozoites incubated in either non-immune bovine colostrum or buffered saline. however oocyst shedding was apparently not reduced in guinea pigs inoculated by gavage with oocysts of c. wrairi and subsequently treated twice daily per os with ...19911818160
cryptosporidium wrairi sp. n. from the guinea pig cavia porcellus, with an emendation of the genus. 19714997038
ultrastructure of cryptosporidium wrairi from the guinea pig. 19714997039
comparison of cryptosporidium parvum and cryptosporidium wrairi by reactivity with monoclonal antibodies and ability to infect severe combined immunodeficient mice.twenty-three monoclonal antibodies raised to cryptosporidium parvum and 12 raised to c. wrairi reacted with equal intensity with the heterologous species. despite demonstration of a close immunologic relationship between these two species, c. wrairi did not induce persistent infection in severe combined immunodeficient mice as did c. parvum.19957806379
similarities and differences between dna of cryptosporidium parvum and c. wrairi detected by the polymerase chain reaction.the objective of this study was to determine if primers, probes, and phc1, a plasmid containing a 2.3 kilobase insert of genomic dna from cryptosporidium parvum, would be useful for the detection of cryptosporidium wrairi dna using the polymerase chain reaction. c. wrairi dna was prepared from oocysts recovered from guinea pigs and c. parvum dna was prepared from the iowa strain of c. parvum. two 26-(bp) primers were used to amplify a 452-base pair bp target sequence within the cloned dna. simil ...19947927066
pcr-rflp analysis of the cryptosporidium oocyst wall protein (cowp) gene discriminates between c. wrairi and c. parvum, and between c. parvum isolates of human and animal origin.cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. despite the morphological and antigenic similarities to c. parvum, c. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species. we used the polymerase chain reaction to clone two distinct 550 bp-long dna fragments, wc-i and wc-ii, of the gene encoding the cryptosporidium oocyst wall protein (cowp) of c. wrairi, which showed 98% identity ...19979170264
phylogenetic analysis of cryptosporidium parasites based on the small-subunit rrna gene locus.biological data support the hypothesis that there are multiple species in the genus cryptosporidium, but a recent analysis of the available genetic data suggested that there is insufficient evidence for species differentiation. in order to resolve the controversy in the taxonomy of this parasite genus, we characterized the small-subunit rrna genes of cryptosporidium parvum, cryptosporidium baileyi, cryptosporidium muris, and cryptosporidium serpentis and performed a phylogenetic analysis of the ...199910103253
the identification of cryptosporidium species and cryptosporidium parvum directly from whole faeces by analysis of a multiplex pcr of the 18s rrna gene and by pcr/rflp of the cryptosporidium outer wall protein (cowp) gene.a multiplex polymerase chain reaction (pcr) procedure to amplify 18s rrna gene fragments has been developed. amplified dna fragments of the expected size were obtained which were specific for cryptosporidium parvum and cryptosporidium wrairi (422 bp), cryptosporidium baileyi (11106 bp) and cryptosporidium muris (1346 bp). criptosporidium parvum and c. wrairi can be distinguished using a pcr/restriction fragment length polymorphism (rflp) analysis of the cryptosporidium outer wall protein (cowp) ...199910576575
molecular analysis of the 18s rrna gene of cryptosporidium serpentis in a wild-caught corn snake (elaphe guttata guttata) and a five-species restriction fragment length polymorphism- based assay that can additionally discern c. parvum from c. adult wild-caught corn snake (elaphe guttata guttata) was presented for humane euthanasia and necropsy because of severe cryptosporidiosis. the animal was lethargic and >5% dehydrated but in good flesh. gastric lavage was performed prior to euthanasia. histopathologic findings included gastric mucosal hypertrophy and a hemorrhagic erosive gastritis. numerous 5- to 7-microm-diameter round extracellular organisms were associated with the mucosal hypertrophy. a pcr, acid-fast stains, giemsa stai ...199910583987
morphologic, host specificity, and genetic characterization of a european cryptosporidium andersoni isolate.this study was undertaken in order to characterize a cryptosporidium muris-like parasite isolated from cattle in hungary and to compare this strain with other cryptosporidium species. to date, the large-type oocysts isolated from cattle were considered as c. muris described from several mammals. the size, form, and structure of the oocysts of the hungarian strain were identical with those described by others from cattle. an apparent difference between the morphometric data of c. muris-like paras ...200011191899
pcr-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair dna fragment discriminates between cryptosporidium parvum and c. meleagridis and between c. parvum isolates of human and animal origin.genomic dnas from human cryptosporidium isolates previously typed by analysis of the 18s ribosomal dna locus (cryptosporidium parvum bovine genotype, c. parvum human genotype, cryptosporidium meleagridis, and cryptosporidium felis) were used to amplify the diagnostic fragment described by laxer et al. (m. a. laxer, b. k. timblin, and r. j. patel, am. j. trop. med. hyg., 45:688-694, 1991). the obtained 452-bp amplified fragments were sequenced and aligned with the homologous cryptosporidium wrair ...200211916736
cloning and phylogenetic analysis of the ribosomal internal transcribed spacer-1 (its1) of cryptosporidium wrairi and its relationship to c. parvum genotypes.we have cloned and sequenced the ribosomal internal transcribed spacer-1 (its1) of cryptosporidium wrairi. phylogenetic analysis of this region provided further support to the validity of c. wrairi as a distinct species and also to the concept that many of the genotypes recently identified within c. parvum are in fact separate species. analysis placed the "cattle" and "mouse" genotypes of c. parvum as each other's closest relatives and c. wrairi as a sister group to these two genotypes, followed ...200112402523
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