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epitheliocystis disease in the striped bass morone saxatilis from the chesapeake bay.epitheliocystis disease in the gills of the striped bass morone saxatilis from chesapeake bay was studied using light and electron microscopy. the epitheliocystis infection appeared synchronous in that all capsules on a single host were at the same stage of development. the disease appeared to begin in a single cell on the gill lamella, which gradually enlarged to form a large cyst, encapsulated by a thick cellular capsule of epithelial origin. the epitheliocystis inclusion was filled with cells ...1977200327
in situ morphology of nitrifying-like bacteria in aquaculture systems.the in situ microbiota from several aquaculture facilities with active nitrification was examined by transmission electron microscopy of thin sections for the presence of bacteria that contained intracytoplasmic membranes characteristic of the nitrifying bacteria. colonies of bacteria with the cellular morphology of a species of nitrosomonas were found to be present in both the culture water and in the biological filter slime of a freshwater chinook salmon (oncorhynchus tshawytscha) culture syst ...1976938036
identification of insulin-like growth factor-binding proteins in the circulation of four teleost fish species.insulin-like growth factor-binding proteins (igf-bps) were demonstrated in the circulation of four teleost fish species. in the coho salmon (oncorhynchus kisutch), serum binding of 125i-labelled human igf-i (125i-higf-i) was competitively inhibited by addition of excess recombinant bovine igf-i (rbigf-i) in a manner similar to that when rat serum was used. western-ligand blot procedure using the same labelled hormone identified at least three major forms of igf-bps in the plasma of all four tele ...19921380067
induction by beta-estradiol of vitellogenin in striped bass (morone saxatilis): characterization and quantification in plasma and mucus.striped bass (morone saxatilis) were implanted with beta-estradiol to induce the production of vitellogenin, the egg yolk precursor produced by the liver. electrophoretic analysis revealed that beta-estradiol caused marked production of a plasma protein of apparent molecular mass 170 kda. size exclusion chromatography suggested that the estradiol-induced protein circulated as a dimer. this protein was purified from the plasma of estradiol-treated fish by deae-agarose column chromatography and us ...19921426961
heterogeneity in the genome rnas and polypeptides of five members of a novel group of rotavirus-like viruses isolated from aquatic animals.biochemical characteristics of five rotavirus-like viruses isolated from striped bass (morone saxatilis), turbot (scophthalmus maximus), smelt (osmerus mordax) and atlantic salmon (salmo salar) in north america and europe were compared. the genome of each isolate was composed of 11 segments of dsrna and each isolate had a unique electropherotype in polyacrylamide gels. agarose gel electrophoresis showed similar rna profiles for all four isolates from north america, whereas the rna profile of the ...19911846647
polychlorobiphenyl (pcb) congeners in striped bass (morone saxatilis) from marine and estuarine waters of new york state determined by capillary gas chromatography.edible filets of striped-bass samples (60) from the hudson river estuary, the atlantic shore of long island, and long island sound were analyzed by high-resolution glass-capillary gas chromatography for 74 polychlorobiphenyl (pcb) compounds. the fish, a non-random subset of a larger sample, contained the following concentrations of total pcb residue (mean +/- the standard error of the mean). the corresponding mean values for the entire survey (n = 717) are given in parentheses: eastern long isla ...19902109967
s-oxygenation of thiobencarb (bolero) in hepatic preparations from striped bass (morone saxatilis) and mammalian systems.the in vitro s-oxygenation of thiobencarb (bolero; p-chlorobenzyl n,n-diethylthiocarbamate) in the presence of hepatic microsomes from freshwater- and seawater-adapted striped bass was investigated. thiobencarb s-oxide was the principal metabolite and accounted for 98% of the total thiobencarb metabolized by striped bass liver microsomes. studies on the biochemical mechanisms for striped bass hepatic s-oxygenation suggest that this reaction is catalyzed largely by the flavin-containing monooxyge ...19902133094
molecular characterization of a rotaviruslike virus isolated from striped bass (morone saxatilis).the characteristics of a rotaviruslike (sbr) virus isolated from striped bass (morone saxatilis) were examined following purification of viruses from infected cell cultures. virions had a double-layered capsid of icosahedral symmetry and a diameter of 75 nm. purified viruses contained five polypeptides ranging in molecular mass from 130 to 35 kda. none of the structural proteins were glycosylated. treatment with edta did not remove the outer capsid. by using enzymes and a chaotropic agent, it wa ...19902170670
effect of exogenous corticosteroids on circulating virus and neutralizing antibodies in striped bass (morone saxatilis) infected with infectious pancreatic necrosis virus.corticosteroids have been reported to induce immunosuppression in fish exposed to many types of bacterial antigens. we document a similar phenomenon in fish exposed to infectious pancreatic necrosis virus (ipnv). fingerling striped bass that were injected with the steroid triamcinolone acetonide (100 mg/kg body weight) 24 hours before receiving intraperitoneal inoculation with ipnv became viremic 3 days post inoculation (dpi) and virus was still detected in the buffy coat cells 14 dpi. in contra ...19863020770
paradoxical effects of cadmium exposure on antibacterial antibody responses in two fish species: inhibition in cunners (tautogolabrus adspersus) and enhancement in striped bass (morone saxatilis).previous work in a marine fish, the cunner (tautogolabrus adspersus), showed that endocytosis of bacteria by cells in the liver and spleen was affected by 96-h exposure of the fish to cadmium at a concentration of 12 micrograms/ml; however, antibody response to sheep erythrocytes was not affected. since the latter finding was questionable because of short immunization times, and data from more than a single fish species were desirable, both the cunner and an anadromous fish, the striped bass (mo ...19863094230
mycobacteriosis in cultured striped bass from california.striped bass (morone saxatilis) juveniles raised in an intensive culture system had chronic mortality resulting from infections with mycobacterium marinum. approximately one-half of a population of 900 yearlings succumbed to the disease and 80% of those remaining were infected. the bacteria were isolated on petrignani's medium after 7 days at 25 c and subcultures grew at temperatures from 20 to 37 c. the disease was characterized by systemic nodular lesions in all major organs. older tubercles c ...19873625895
transition metal-binding proteins from three chesapeake bay fish species.three species of chesapeake bay fish were collected, and endogenous levels of metal binding protein (mbp) were determined. in addition, the induction of metal-binding proteins by cadmium was studied. livers from freshly caught fish were extracted and chromatographed on sephadex g-75 to resolve mbp in the 5 to 20 kdalton range. all species studied exhibit measurable but varied levels of endogenous mbps in the molecular weight range investigated, mostly as a copper protein complex. upon induction ...19863709435
quantitative and qualitative studies of gut flora in striped bass from estuarine and coastal marine environments.examination of the intestinal contents of 130 striped bass (morone saxatilis) collected from the hudson river and long island sound during may to october 1981 showed that opportunistic fish pathogens--especially aeromonas hydrophila--predominated in samples from both locations. other isolates from both groups of striped bass included vibrio, pseudomonads, flavo-bacteria, alcaligenes, and enterics. small numbers of micrococcus, bacillus, corynebacterium, and acinetobacter were also isolated. tota ...19863735580
ultrastructural observations on peritoneal exudate cells from the striped bass.an ultrastructural study was conducted of the principal cells recovered from the peritoneal exudate of striped bass (morone saxatilis) injected with killed bacillus cereus. the most frequently observed cells were identified as macrophages, eosinophils, and neutrophils on the basis of comparing their structure with that reported for other fish species and higher vertebrates. all three of the types of cells were phagocytic; however, the numbers of bacteria engulfed were limited. like other studies ...19863765336
neutralizing activity against infectious pancreatic necrosis virus in striped bass, morone saxatilis, from the chesapeake bay. 19873820417
a light and electron microscopic study of epitheliocystis disease in the gills of connecticut striped bass (morone saxatilis) and white perch (morone americanus). 19704099449
toxicity of bromate to striped bass ichthyoplankton (morone saxatilis) and juvenile spot (leiostomus xanthurus).striped bass (morone saxatillis) eggs (12 h after fertilization) and larvae (4 d after hatching) and juvenile spot (leiostomus xanthurus) were exposed to a series of bromate concentrations for 4, 10, and 10 d, respectively, using static replacement bioassay techniques. three-dimensional mortality response surfaces were constructed by computerized probit regression techniques. newly hatched striped bass prolarvae were most sensitive to bromate and had a 96-h lc50 of 30.8 mg/l (as bro3-). four-day ...19817338936
the immunocytochemistry of cytokeratin in fish tissues.an increasing interest in fish species as sentinels of environmental pollution and in carcinogenesis research has led to the identification of diagnostically challenging neoplasms of uncertain cellular origin and the need for additional diagnostic methods. to determine the potential of using commercially available antibodies to intermediate filament proteins on paraffin-embedded fish tissues for immunocytochemistry in tumor diagnosis, the application of three antikeratin antibodies to normal adu ...19937505508
production of a biologically active recombinant teleostean growth hormone in e. coli cells.we have isolated and characterized several recombinant lambda phage clones carrying growth hormone (gh) cdna of striped bass (morone saxatilis). nucleotide sequence and the predicted amino acid sequence of sbgh was determined from a recombinant clone carrying the longest cdna insert. the sbgh cdna encodes a pre-hormone of 204 amino acid residues. comparison of the predicted amino acid sequence of sbgh with those of other vertebrates revealed different degrees of sequence identity: approximately ...19957758842
hormonal regulation of final maturation of striped bass oocytes in vitro.an in vitro culture system was developed to investigate hormonal control of final oocyte maturation (fom) in striped bass (morone saxatilis). isolated ovarian fragments exposed to human chorionic gonadotropin (hcg), dibutyryl camp, or forskolin produced significant amounts of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (dhp) and the oocytes underwent germinal vesicle breakdown (gvbd). slight increases in 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-s) production were also observed. pro ...19947851722
variability in an mhcmosa class ii beta chain-encoding gene in striped bass (morone saxatilis).the major histocompatibility complex (mhc) class ii b locus of the striped bass (morone saxatilis) was found to contain multiple forms of the class ii b gene. seven complete mhc class ii b cdna clones were isolated and sequenced, identifying five unique allelic forms of a mhc class ii b gene. among three specimens, each representing a geographically distinct population (chesapeake bay, md; roanoke river, nc; and santee-cooper reservoir, sc) extensive variability was detected in the beta 1 encodi ...19947883062
survey of hox-like genes in the teleost morone saxatilis: implications for evolution of the hox gene family.we used a pcr-based method to survey a defined subset of hox-like genes in the genome of morone saxatilis (striped bass) to determine their relationship to similar hox genes in other vertebrates, to estimate hox cluster number, and to gain additional information about vertebrate hox gene evolution. we identified eight distinct striped bass hox-like genes representing hox clusters a, b, and c. comparison of hox gene evolutionary trees from striped bass, zebrafish, and mouse provided evidence cons ...19947921046
growth hormone- and prolactin-producing cells in the pituitary gland of striped bass (morone saxatilis): immunocytochemical characterization at different life stages.the striped bass (morone saxatilis) is a seasonally breeding, long-lived, anadromous fish of growing economic importance. to describe the apparent activities of growth hormone (gh)- and prolactin (prl)-producing cells, pituitaries were collected from captive juveniles and from wild adult fish in late spring off the rhode island coast during their coastal migration and in the hudson river during the spawning migration. gh and prl were separated by reversed-phase hplc of pituitary extracts from ca ...19947926632
plasma levels of gonadal steroids during final oocyte maturation of striped bass, morone saxatilis l.levels of estradiol-17 beta (e2), testosterone (t), 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (dhp), and 17 alpha,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-s) were measured by radioimmunoassay (ria) in blood plasma of striped bass undergoing final oocyte maturation (fom). females were captured just prior to, or in the early stages of, fom and induced to complete maturation and ovulation with injected human chorionic gonadotropin, synthetic salmon gonadotropin-releasing hormone analogue (sg ...19947958748
cold acclimation increases carnitine palmitoyltransferase i activity in oxidative muscle of striped bass.the effect of thermal acclimation on the activity of carnitine palmitoyltransferase i (cpt i), the rate-limiting enzyme for beta-oxidation of long-chain fatty acids, was determined in oxidative red muscle of striped bass (morone saxatilis) acclimated at 5 or 25 degrees c. as observed in mammalian tissues, malonyl-coa potently inhibited cpt i activity of mitochondria. inhibition by malonyl-coa required inclusions of both bovine serum albumin (bsa) and palmitoyl-coa in the reaction media. because ...19948141397
in vitro secretion of insulin-like growth factor-binding proteins from liver of striped bass, morone saxatilis.in vitro secretion of insulin-like growth factor-binding proteins (igfbps) from liver of striped bass (sb: morone saxatilis) was studied using a simple organ-culture system. liver cubes (1 mm3) were cultured in minimum essential medium with earle's salts containing 0.1% bovine serum albumin and 100 u/ml penicillin in 5% co2/95% o2 at 16 degrees. the amount of double-stranded dna in these cultured liver cubes did not change by 192 hr in the culture, but decreased by 216 hr. four igfbps (a 23- to ...19958536935
a low-molecular-weight (25-kda) igf-binding protein is increased with growth inhibition in the fasting striped bass, morone saxatilis.the effect of fasting on circulating igfbps in the striped bass was assessed in relation to changes in growth and metabolism. thirty-day-fasted (30df) and 60-day-fasted (60df) fish, and 60df fish refed for 14 additional days (refed), were compared with control, fed fish. growth and metabolic status of each animal were assessed by determining body length (bl) and body weight (bw) changes, hepatosomatic index (hsi), condition factor (cf), and serum glucose concentration, and by assaying for incorp ...19968804561
humoral immunoglobulins of the white sturgeon, acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.white sturgeon (acipenser transmontanus) immunoglobulin (ig) was purified from serum by two methods, ion-exchange chromatography and gel filtration and precipitation of the euglobulin fraction. the purity of these immunoglobulin preparations was confirmed by gel electrophoresis. sequence analysis of the n-terminal amino acids confirmed that the purified protein was immunoglobulin. the major portion of the immunoglobulin preparation consisted of two proteins with estimated molecular weights (m.w. ...19968915630
the effect of temperature and water quality on antibody response to aeromonas salmonicida in sunshine bass (morone chrysops x morone saxatilis).the kinetics of the antibody response to aeromonas salmonicida were determined in sunshine bass (hybrid striped bass: female morone chrysops x male morone saxatilis) acclimated to 10, 18, 24, 29 degrees c, and to 0.15 mg 1(-1) un-ionized ammonia and 200 mg 1(-1) nitrate levels. temperature and water quality factors affected the immune response of sunshine bass. temperatures of 10 degrees c and 18 degrees c decreased the magnitude and delayed the time of the antibody response to a. salmonicida. t ...19969157680
edwardsiellosis in wild striped bass from the chesapeake bay.the first epizootic of edwardsiellosis, caused by edwardsiella tarda, is described. the epizootic occurred in the chesapeake bay, maryland (usa) during the summer and autumn of 1994, and affected wild adult striped bass (morone saxatilis). clinical signs included numerous irregular coalescing hemorrhagic ulcers on the body and fins that were distinctly malodorous. internally, the body cavity was filled with abundant yellowish or sanguinous mucoid fluid, and the visceral organs had multiple tiny ...19979249698
plasma gonadotropin ii, sex steroids, and thyroid hormones in wild striped bass (morone saxatilis) during spermiation and final oocyte maturation.the blood levels of gonadotropin ii (gth ii), sex-steroid hormones, and thyroid hormones were determined in wild spermiating male striped bass (morone saxatilis) in males and in females at various stages of final oocyte maturation (fom), captured on their spawning grounds. the progression of spermiation was associated with increases in plasma gth ii and decreases in plasma testosterone (t), 11-ketotestosterone, and thyroxine (t4). plasma triiodothyronine (t3) remained at high and relatively unch ...19979356218
endocrine profiles of female striped bass (morone saxatilis) in captivity, during postvitellogenesis and induction of final oocyte maturation via controlled-release gnrha-delivery systems.plasma levels of reproductive and thyroid hormones were measured in captive striped bass females during postvitellogenesis and the spawning period (march-june). circulating gonadotropin ii (gth ii), 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-p), and 17,20beta, 21-trihydroxy-4-pregnen-3-one (17,20beta,21-p) remained low and unchanged in nonmaturing females, while 17beta-estradiol (e2) and testosterone (t) declined throughout postvitellogenesis. plasma thyroxine (t4) declined significantly in ...19989593648
fish lesions in the chesapeake bay: pfiesteria-like dinoflagellates and other etiologies.ulcerative lesions and mass mortalities of atlantic estuarine fish, particularly menhaden (brevoortia tyrannus), have been associated with exposure to pfiesteria-like dinoflagellates and their toxins. we collected fish from the chicamacomico river, maryland, and observed solitary ulcerative lesions on the majority of menhaden sampled. one striped bass (morone saxatilis) had an area of reddening around the base of the dorsal fin. bluegill (lepomis machrochirus), channel catfish (ictalurus punctat ...19989601194
a comparison of the chemiluminescent response of crassostrea virginica and morone saxatilis phagocytes to zymosan and viable listonella anguillarum.if reactive oxygen species (ros) produced by hemocytes of the eastern oyster, crassostrea virginica, impart bactericidal activity, exposure of hemocytes to bacteria should result in increased ros generation. in an earlier study, this hypothesis was tested using luminol- and lucigenin-augmented chemiluminescence (cl) to measure ros production. the bacterium listonella anguillarum did not stimulate a net increase in hemocyte-derived cl, and it was suggested that bacterial antioxidants might suppre ...19989617583
indicative and pathogenic microbiological quality of aquacultured finfish grown in different production systems.the nature and number of indicator and pathogenic microbes in fish reared using recirculating and nonrecirculating water systems were compared. for each system, 20 samples of rainbow trout (oncorhynchus mykiss), tilapia (oreochromis spp.), hybrid striped bass (morone saxatilis x m. chrysops), and pacu (piaractus mesopotamicus) were randomly selected and gutted, and microbial analyses were performed using aoac procedures. five fish were subsampled and analyzed for indicative microbial quality wit ...19989708283
multiple gnrhs present in a teleost species are encoded by separate genes: analysis of the sbgnrh and cgnrh-ii genes from the striped bass, morone saxatilis.gnrh is a neuropeptide which plays an essential role in the control of reproductive fitness for all vertebrates. increasing evidence suggests that multiple forms of gnrh may exist in most vertebrate brains. southern blot analysis of the three gnrhs known to be present in perciform fish, the seabream (sb)gnrh, the salmon(s) gnrh and the chicken (c) gnrh-ii, demonstrates that each is present as a single gene copy in the genome of the striped bass, morone saxatilis. in order to investigate the phys ...19989845669
genomic organization of the hoxa4-hoxa10 region from morone saxatilis: implications for hox gene evolution among vertebrates.the physical mapping of hox gene clusters from a limited number of vertebrates has shown an overall conservation in gene organization in which major evolutionary changes appear to be primarily restricted to the deletion of one or more genes, with the exception of the amplification of additional clusters as postulated from zebrafish. we have sequenced a 31 kb region of the hoxa cluster from the teleost morone saxatilis (striped bass), both to provide a detailed physical map of this region and to ...199910327649
novel myosin vi isoform is abundantly expressed in retina.several forms of sensory deficit have been associated with unconventional myosin defects in humans and other animals. normal hearing in mammals has been shown to require functional myosin vi (avraham et al., 1995) and myosin viia (gibson et al., 1995; liu et al., 1997), and the combined blindness and deafness of usher syndrome type ib has been shown to be produced by specific defects in myosin viia (weil et al., 1997). here we report the cloning and characterization of two distinct myosin vi iso ...200010644428
comparative severity of experimentally induced mycobacteriosis in striped bass morone saxatilis and hybrid tilapia oreochromis spp.twenty striped bass morone saxatilis and 20 hybrid tilapia oreochromis niloticus x o. mossambicus x o. aureus each received a single intramuscular injection of 1.6 x 10(6) colony forming units per gram body weight of mycobacterium marinum. striped bass manifested significantly greater clinical and microscopic disease compared to tilapia. whereas all the striped bass had died or were clinically ill by day 8 post-infection, there was no apparent disruption of normal behaviour, physical appearance, ...199910686670
a survey of size-specific mercury concentrations in game fish from maryland fresh and estuarine waters.a survey of size-specific mercury (hg) concentrations in game fish from a subset of maryland fresh and estuarine waters was conducted, in which hg concentrations in 112 fish from seven freshwater impoundments and three tidal and four estuarine locations in chesapeake bay and its tributaries were measured. striped bass (morone saxatilis) was the most intensively examined species. of the fish examined, the largest freshwater sportfish contained the highest hg concentrations. striped bass and large ...200010790502
cloning and sequencing hybrid striped bass (morone saxatilis x m. chrysops) transforming growth factor-beta (tgf-beta), and development of a reverse transcription quantitative competitive polymerase chain reaction (rt-qcpcr) assay to measure tgf-beta mrna of teleost fish.a transforming growth factor (tgf)-beta was isolated and cloned from hybrid striped bass (morone saxatilis x m. chrysops) anterior kidney mononuclear cells. this isolate (genbank accession number af140363) contains an open reading frame of 1146 bases coding for a 382 amino acid protein most similar to rainbow trout tgf-beta (57.3 and 78.6% identity with precursor and active protein, respectively) and rat tgf-beta 1 (41.1 and 68.8% identity with precursor and active protein, respectively). consen ...200010938723
effects of insulin-like growth factor-i on in vitro final oocyte maturation and ovarian steroidogenesis in striped bass, morone saxatilis.recombinant human (rh) insulin-like growth factor-i (igf-i) was more potent than rhigf-ii at inducing in vitro germinal vesicle breakdown (gvbd), a marker for resumption of meiosis, in oocytes of striped bass. treatment of ovarian fragments containing oocytes in intact follicles with rhigf-i increased concentrations of estradiol-17beta and maturation-inducing steroid (mis) 17,20beta, 21-trihydoxy-4-pregnen-3-one (20beta-s) in the culture medium and decreased testosterone levels. the follicles we ...200010993826
cloning and functional expression of a thyrotropin receptor from the gonads of a vertebrate (bony fish): potential thyroid-independent role for thyrotropin in reproduction.the thyroid stimulating hormone receptor (tshr) mediates the pituitary control of the development, growth and function of the thyroid. the expression of the gene encoding this receptor is known only in the thyroid, lymphocytes, fibroblasts, retro-orbital tissue and fat cells. we have cloned a tshr from the gonads of a non-mammalian vertebrate, a bony fish (striped bass, morone saxatilis) in the course of our search for gonadotropin receptors (follicle stimulating hormone receptor, fshr and lutei ...200011000515
characterization of a pituitary gnrh-receptor from a perciform fish, morone saxatilis: functional expression in a fish cell line.gonadotropin-releasing hormones (gnrhs) bind to the specific receptor present on the gonadotrophs to activate the synthesis and release of gonadotropins (follicle stimulating hormone or fsh and luteinizing hormone or lh), which in turn control gonadal maturation, gametogenesis and gamete release. perciform species have three endogenous gnrhs. the main objective of this study was to characterize the gonadotropin-releasing hormone receptor (gnrh-r) present in the pituitary of a perciform species, ...200011064153
seasonal fluctuations in pituitary levels of the three forms of gonadotropin-releasing hormone in striped bass, morone saxatilis (teleostei), during juvenile and pubertal development.in most vertebrates, the development of a mature gonadotropin-releasing hormone (gnrh) secretory system is pivotal for the onset of puberty. the role of the three native gnrh forms, seabream (sb) gnrh, chicken (c) gnrh-ii and salmon gnrh, in striped bass puberty remains elusive. this study examined the changes in pituitary gnrh levels throughout juvenile and pubertal development, a period encompassing 3 to 4 years. the levels of the two most abundant forms in the pituitary, sbgnrh and cgnrh-ii ( ...200111375123
in vitro hormone induction of final oocyte maturation in striped bass (morone saxatilis) follicles is inhibited by blockers of phosphatidylinositol 3-kinase activity.oocyte germinal vesicle breakdown (gvbd) was induced in striped bass ovarian fragments when tissues were incubated with 100-nm recombinant human insulin-like growth factor-i (rhigf-i), 25-iu human chorionic gonadotropin (hcg) ml(-1), or 290 nm of the maturation-inducing steroid (mis), 17,20beta,21-trihydroxy-4-pregnen-3-one (20beta-s). inhibitors of phosphatidylinositol 3-kinase (pi 3-k), wortmannin (100 nm) and ly 294002 (50 microm), inhibited gvbd induced by these hormones. furthermore, the in ...200111399481
sequence conservation among fish myostatin orthologues and the characterization of two additional cdna clones from morone saxatilis and morone americana.myostatin (mstn) negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. mice and cattle possessing mutant mstn alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. mstn orthologues have been previously characterized in 12 vertebrate species, including the white bass morone chrysops. presented herein is the identification and characterization of novel cdna clones from two additio ...200111399495
development of hepatic cyp1a and blood vitellogenin in eel (anguilla anguilla) for use as biomarkers in the thames estuary, uk.the potential of eel (anguilla anguilla) as a monitoring species for the thames estuary, uk, was examined. hepatic cytochrome p4501a [7-ethoxyresorufin o-deethylase (erod) activity] and blood vitellogenin (western analysis) were investigated as biomarkers of exposure to, respectively, organic contaminants and to contaminants showing estrogenic activity. hepatic microsomal erod activities in a. anguilla from seven sites in the thames estuary in may 1998 varied three-fold (111 +/- 24 to 355 +/- 42 ...200011460720
a unique mycobacterium species isolated from an epizootic of striped bass (morone saxatilis).we isolated a mycobacterium sp. resembling mycobacterium marinum and m. ulcerans from diseased striped bass (morone saxatilis) during an epizootic of mycobacteriosis in the chesapeake bay. this isolate may represent an undescribed mycobacterium species, based on phenotypic characteristics and comparative 16s rrna gene sequence.200111747708
the gnrh system in the european sea bass (dicentrarchus labrax).the cdna sequences encoding three gnrh forms, sea bream gnrh (sbgnrh), salmon gnrh (sgnrh) and chicken gnrh ii (cgnrh ii), were cloned from the brain of european sea bass, dicentrarchus labrax. comparison of their deduced amino acid sequences to the same forms in the gilthead sea bream, sparus aurata, and striped bass, morone saxatilis, revealed high homology of the prepro-cgnrh ii (94% and 98% respectively), and prepro-sgnrh (92% to both species). the sbgnrh exhibited dissimilar identities, wit ...200211786378
molecular taxonomy of the suborder bodonina (order kinetoplastida), including the important fish parasite, ichthyobodo necator.ichthyobodo necator is an important fish ectoparasite with a broad host and ecological range. a novel method, involving the use of an anesthetic, allowed the collection of large numbers of parasites from the skin and gills of hybrid striped bass (morone saxatilis male x m. chrysops female). genomic dna from these samples was used to amplify and clone the 18s rrna gene. the 18s rrna gene was similarly cloned from bodo caudatus, bodo edax, bodo saltans, an unidentified bodo species, and dimastigel ...200212046598
fish tissue quality in the lower mississippi river and health risks from fish consumption.between 1990 and 1994, samples of three shellfish species (i.e. blue crab, callinectes sapidus;crayfish, procambarus acutis; and river shrimp, macrobrachium ohionii) and 16 fish species and were collected at six sites along the lower mississippi river by the louisiana department of environmental quality, office of water resources in coordination with the us environmental protection agency. the fish species included: bigmouth buffalo (ictiobus cyanellus); blue catfish (ictalurus furcatus); carp ( ...200312526903
mycobacterium shottsii sp. nov., a slowly growing species isolated from chesapeake bay striped bass (morone saxatilis).slowly growing, non-pigmented mycobacteria were isolated from striped bass (morone saxatilis) during an epizootic of mycobacteriosis in the chesapeake bay. growth characteristics, acid-fastness and results of 16s rrna gene sequencing were consistent with those of the genus mycobacterium. a unique profile of biochemical reactions was observed among the 21 isolates. a single cluster of eight peaks identified by analysis of mycolic acids (hplc) resembled those of reference patterns but differed in ...200312710607
experimental mycobacteriosis in striped bass morone saxatilis.striped bass morone saxatilis were infected intraperitoneally with approximately 10(5) mycobacterium marinum, m. shottsii sp. nov., or m. gordonae. infected fish were maintained in a flow-through freshwater system at 18 to 21 degrees c, and were examined histologically and bacteriologically at 2, 4, 6, 8, 17, 26, 36 and 45 wk post-infection (p.i.). m. marinum caused acute peritonitis, followed by extensive granuloma development in the mesenteries, spleen and anterior kidney. granulomas in these ...200312747636
transforming growth factor-beta response to mycobacterial infection in striped bass morone saxatilis and hybrid tilapia oreochromis spp.striped bass (morone saxatilis) and hybrid tilapia (oreochromis spp.) were experimentally infected with mycobacterium marinum. splenic mononuclear cell transforming growth factor-beta (tgf-beta) mrna was measured by reverse transcription quantitative-competitive pcr (rt-qcpcr). in histologic sections of liver and anterior kidney, the area of each section that was occupied by granulomas and the total area of each section were measured by computer-assisted image analysis and compared as a proporti ...200312963276
the effect of storing hybrid bass (morone saxatilis x m. chrysops) sera at freezing temperatures on antibody titers.the effect of storing hybrid bass sera at freezing temperatures on their enzyme-linked immunosorbent assay (elisa) titers was determined, and the precipitate formed during storage was characterized. frozen bass sera from fish vaccinated with bacterial sonicate showed no titer in either of two groups-one immunized with bacterial sonicate and the other with sonicate emulsified in adjuvant. fresh sera exhibited average titers of 1:32 in both groups and 1:20 in convalescent serum. hybrid bass antise ...200312969655
mycobacterium-inducible nramp in striped bass (morone saxatilis).in mammals, the natural resistance-associated macrophage protein 1 gene, nramp1, plays a major role in resistance to mycobacterial infections. chesapeake bay striped bass (morone saxatilis) is currently experiencing an epizootic of mycobacteriosis that threatens the health of this ecologically and economically important species. in the present study, we characterized an nramp gene in this species and obtained evidence that there is induction following mycobacterium exposure. the striped bass nra ...200414977970
polymicrobic infection of hybrid striped bass (morone chrysops x morone saxatilis) with three bacterial pathogens: a case report. 200415009255
chlordane contamination in selected estuarine and coastal marine finfish and shellfish of new jersey, usa.analysis of chlordane contamination in selected finfish and shellfish species from estuarine and coastal marine waters of new jersey, usa, indicates consistently highest organochlorine pesticide levels in samples from the north and northeast regions of the state. gas chromatographic analysis of tissue samples from four finfish species (american eel, anguilla rostrata; bluefish, pomatomus saltatrix; striped bass, morone saxatilis; and weakfish, cynoscion regalis) and one shellfish species (blue c ...199615093520
dietary oligonucleotides from yeast rna influence immune responses and resistance of hybrid striped bass (morone chrysops x morone saxatilis) to streptococcus iniae infection.three feeding trials were conducted to evaluate potential immunomodulatory effects of nucleotides in the diet of hybrid striped bass. a basal diet was formulated from menhaden fish meal to contain 40% crude protein and 10% lipid. an oligonucleotide product (ascogen p) from brewer's yeast was added to the basal formulation at the manufacturer's recommended rate of 0.5% to produce the experimental diet. each diet was fed to four replicate groups of juvenile hybrid striped bass for seven or eight w ...200415110330
the effect of growth medium salinity of photobacterium damselae subsp. piscicida on the immune response of hybrid bass (morone saxatilis x m. chrysops).photobacterium damselae subsp. piscicida (p. damselae) was grown on various media and the effect of media salinity on certain immune responses of hybrid bass was studied. in israel, pasteurellosis outbreaks have not been reported at water salinities below 1.38 per thousand. during vaccination experiments the salinity of the medium on which p. damselae is grown, was shown to affect stimulation of the immune system. no correlation was found between antibody response and protection. bacterial envel ...200415123315
age related changes in humoral immune response of hybrid striped bass (morone chrysops x morone saxatilis).a number of factors can influence immune function affecting the magnitude and duration of specific immune responses. one factor which has been noted to affect immune responses is age of animal. in mammals, juveniles have a lower immune response when compared to adults. in fish, fry have a lower immune response than adults; however, few studies have investigated the immune response in juveniles, the age when most fish are reared in aquaculture settings. the humoral immune responses of hybrid stri ...200415261696
species differences in contaminants in fish on and adjacent to the oak ridge reservation, tennessee.risks to humans and other organisms from consuming fish have become a national concern in the usa. in this paper, we examine the concentrations of 137cs, arsenic, beryllium, cadmium, lead, mercury, and selenium in three species of fish from two river reaches adjacent to the us department of energy's oak ridge reservation in tennessee. we were interested in whether there were species and locational differences in radiocesium and metal concentrations and whether concentrations were sufficiently hi ...200415325875
in vitro response of the striped bass natural resistance-associated macrophage protein, nramp, to lps and mycobacterium marinum exposure.mycobacteriosis in chesapeake bay (usa) striped bass morone saxatilis is an ongoing disease problem with important economic implications for a large commercial and recreational fishery. additionally, striped bass serve as a reservoir of potential mycobacterial zoonoses. recently, we described a striped bass gene homolog of the natural resistance-associated macrophage protein family (msnramp), which is responsible for resistance to mycobacterial infections in mice. striped bass msnramp is strongl ...200415533797
bass hepcidin synthesis, solution structure, antimicrobial activities and synergism, and in vivo hepatic response to bacterial infections.bass hepcidin was purified from the gill of hybrid striped bass (morone chrysops x morone saxatilis) based on antimicrobial activity against escherichia coli. this 21-amino acid peptide has 8 cysteines engaged in 4 disulfide bonds and is very similar to human hepcidin, an antimicrobial peptide with iron regulatory properties. to gain insight into potential role(s) of bass hepcidin in innate immunity in fish, we synthesized the peptide, characterized its antimicrobial activities in vitro, determi ...200515546886
effect of salinity on expression of branchial ion transporters in striped bass (morone saxatilis).the time course of osmoregulatory adjustments and expressional changes of three key ion transporters in the gill were investigated in the striped bass during salinity acclimations. in three experiments, fish were transferred from fresh water (fw) to seawater (sw), from sw to fw, and from 15-ppt brackish water (bw) to either fw or sw, respectively. each transfer induced minor deflections in serum [na+] and muscle water content, both being corrected rapidly (24 hr). transfer from fw to sw increase ...200415562450
isolation and characterization of mycobacteria from striped bass morone saxatilis from the chesapeake bay.mycobacteriosis in striped bass morone saxatilis of chesapeake bay, usa, was first diagnosed in 1997 based on the presence of granulomatous inflammation and acid-fast bacteria in skin and spleen. to confirm histopathology, bacteriological detection and identification of mycobacteria were begun using splenic tissue from fish with and without skin ulcerations. on the basis of initial studies using a variety of selective and nonselective media, decontamination, homogenization and incubation conditi ...200415584409
seasonal, interannual, and long-term variation in sport fish contamination, san francisco bay.this study documents changes in contamination over time at seasonal, interannual, and decadal time scales for sport fish collected in san francisco bay. samples from seven fish species were prepared according to common consumption practices (muscle fillets either with or without skin) and analyzed for trace metals (mercury and selenium) and trace organochlorine contaminants (pcbs, ddts, chlordanes, and dieldrin). in 2000, sport fish samples exceeded human health screening values for mercury, pcb ...200515589247
immunogenic and protective effects of a dna vaccine for mycobacterium marinum in fish.mycobacteriosis, caused by numerous mycobacterium spp., can be a devastating disease of both wild and cultured fishes. as no efficacious treatment exists, a vaccine against fish mycobacteriosis is essential for prevention and control of this disease. thus, a dna vaccine was constructed using the mycobacterium marinum ag85a gene that encodes one of the major secreted fibronectin-binding proteins of mycobacterium spp., which was isolated and then subcloned into a commercially available eukaryotic ...200515621306
ultrastructure of mycobacterium marinum granuloma in striped bass morone saxatilis.an emerging epizootic of mycobacteriosis currently threatens striped bass morone saxatilis populations in chesapeake bay, usa. several species of mycobacteria, including mycobacterium marinum, species resembling m. avium, m. gordonae, m. peregrinum, m. scrofulaceum and m. terrae, and the new species m. shottsii have been isolated from diseased and healthy bass. in this study, we describe the ultrastructure of developing m. marinum granulomas in experimentally infected bass over a period of 45 wk ...200415648839
optimal swim speeds for traversing velocity barriers: an analysis of volitional high-speed swimming behavior of migratory fishes.migrating fish traversing velocity barriers are often forced to swim at speeds greater than their maximum sustained speed (u(ms)). failure to select an appropriate swim speed under these conditions can prevent fish from successfully negotiating otherwise passable barriers. i propose a new model of a distance-maximizing strategy for fishes traversing velocity barriers, derived from the relationships between swim speed and fatigue time in both prolonged and sprint modes. the model predicts that fi ...200515671330
mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from chesapeake bay striped bass (morone saxatilis).a group of slowly growing photochromogenic mycobacteria was isolated from chesapeake bay striped bass (morone saxatilis) during an epizootic of mycobacteriosis. growth characteristics, acid-fastness and 16s rrna gene sequencing results were consistent with those of the genus mycobacterium. biochemical reactions, growth characteristics and mycolic acid profiles (hplc) resembled those of mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. sequencing of th ...200515879246
molecular identification and expression analysis of natural resistance associated macrophage protein (nramp) cdna from japanese flounder (paralichthys olivaceus).natural resistance associated macrophage protein (nramp) controls partially innate resistance to intracellular parasites. its function is to enhance the ability of macrophages to kill pathogens. however, little is known about the structure and function of nramp in lower vertebrates such as teleosts. we have recently isolated a cdna encoding nramp from japanese flounder (paralichthys olivaceus). the full-length cdna of the nramp is 3066 bp in length, including 224 bp 5' terminal utr, 1662 bp enco ...200615998592
molecular polymorphism and expression analysis of mhc class ii b gene from red sea bream (chrysophrys major).mhc class ii (major histocompatibility complex class ii) plays an important role in the immune response of vertebrates. its function is to present antigenic peptides to the t-cell receptor. in order to study the function and molecular polymorphism of class ii b gene in fish, we have isolated cdnas encoding class ii b from spleen cdna library of red sea bream (chrysophrys major) by using est sequencing, and examined genomic organization, molecular polymorphism and expression of red sea bream clas ...200616045985
comparative analysis of mycobacterial infections in wild striped bass morone saxatilis from chesapeake bay.during an ongoing epizootic of mycobacteriosis, wild striped bass morone saxatilis from chesapeake bay were analyzed using 3 methods for detection of either mycobacterial infection or associated granulomatous pathology. the specific detection techniques, which utilized aseptically collected splenic tissue, were histology, quantitative culture and nested pcr. based on analysis of 118 samples, detection of infection differed significantly between the 3 methods (chi-square, p = 0.0007). quantitativ ...200516385818
molecular identification, polymorphism, and expression analysis of major histocompatibility complex class iia and b genes of turbot (scophthalmus maximus).major histocompatibility complex (mhc) class ii has a central role in the adaptive immune system by presenting foreign peptides to the t-cell receptor. the full lengths of mhc class ii a and b cdna were cloned from turbot by homology cloning and rapid amplification of cdna ends polymerase chain reaction (race pcr), and genomic organization, molecular polymorphism, and expression of turbot class iib gene were examined to study the function of class iib gene in fish. the deduced amino acid sequenc ...200616832747
immune and histopathologic responses of dna-vaccinated hybrid striped bass morone saxatilis x m. chrysops after acute mycobacterium marinum infection.the post-challenge immune and histopathologic responses of hybrid striped bass vaccinated with a dna vaccine encoding the mycobacterium marinum ag85a gene and subsequently challenged with m. marinum were investigated. juvenile hybrid striped bass morone saxatilis x m. chrysops were injected intramuscularly with 25 or 50 microg dna plasmid and developed significant specific protective responses to live bacterial challenge 120 d post-vaccination. both vaccine groups demonstrated increased survival ...200617240750
cloning and expression analysis of myogenin from flounder (paralichthys olivaceus) and promoter analysis of muscle-specific expression.myogenin is a bhlh transcription factor of the myod family. it plays a crucial role in myoblast differentiation and maturation. we report here the isolation of flounder myogenin gene and the characterization of its expression patterns. sequence analysis indicated that flounder myogenin shared a similar structure and the conserved bhlh domain with other vertebrate myogenin genes. flounder myogenin gene contains 3 exons and 2 introns. sequence alignment and phylogenetic showed that flounder myogen ...200717336560
isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern canada.viral haemorrhagic septicaemia virus (vhsv) was isolated from mortalities occurring in populations of mummichog, fundulus heteroclitus, stickleback, gasterosteus aculeatus aculeatus, brown trout, salmo trutta, and striped bass, morone saxatilis, in new brunswick and nova scotia, canada. the isolated viral strains produced a cytopathic effect on the epithelioma papillosum cyprini cell line. serum neutralization indicated the virus was vhsv and sequencing identified the rhabdovirus isolates as the ...200717394523
mercury in sport fish from the sacramento-san joaquin delta region, california, usa.total mercury (hg) concentrations were determined in fillet tissue of sport fish captured in the sacramento-san joaquin river delta and surrounding tributaries, a region particularly impacted by historic gold and mercury mining activity. in 1999 and 2000, mercury concentrations were measured in 767 samples from ten fish species. largemouth bass (micropterus salmoides), the primary target species, exhibited a median hg concentration of 0.53 mug g(-1) (n=406). only 23 largemouth bass (6%) were bel ...200818063015
mycobacterial infections in striped bass from delaware bay.eighty striped bass morone saxatilis were obtained from delaware bay using commercial gill nets set adjacent to woodland beach (n = 70) and bowers beach (n = 10) in december 2003. fish were examined for gross lesions. total lengths (tls) and eviscerated weights were determined to calculate condition factors (k). portions of spleens were aseptically harvested for bacterial culture, and portions of spleens, kidneys (anterior and posterior), livers, and gonads were obtained for histological examina ...200718201050
comparison of antimicrobial activity in the epidermal mucus extracts of fish.the mucus layer on the surface of fish consists of several antimicrobial agents that provide a first line of defense against invading pathogens. to date, little is known about the antimicrobial properties of the mucus of arctic char (salvelinus alpinus), brook trout (s. fontinalis), koi carp (cyprinus carpio sub sp. koi), striped bass (morone saxatilis), haddock (melanogrammus aeglefinus) and hagfish (myxine glutinosa). the epidermal mucus samples from these fish were extracted with acidic, orga ...200818342561
mycobacteriosis in fishes: a review.mycobacterium species have long been recognised as a significant source of morbidity and mortality in finfish aquaculture, as well as in wild finfishes. mycobacteria infecting fishes also include zoonotic pathogens that can cause protracted illness, especially in immunocompromised individuals. several basic aspects of mycobacterial pathobiology in aquatic animals remain poorly understood, although a number of important recent developments have been made, especially with respect to identification ...200918620877
apolipoprotein a-i from striped bass (morone saxatilis) demonstrates antibacterial activity in vitro.hdl and apolipoprotein a-i from teleostean fishes demonstrate in vitro activity against gram-positive and gram-negative bacteria. in this study, we purified apoa-1 from striped bass (morone saxatilis) plasma and examined its in vitro antibacterial activity against streptococcus sp., escherichia coli, and mycobacterium marinum. in addition, we obtained sequence for a putative striped bass apoa-1 gene, which when translated contained the identical sequence generated from n-terminal sequencing of t ...200818627791
mycobacteriosis-associated mortality in wild striped bass (morone saxatilis) from chesapeake bay, u.s.a.the striped bass (morone saxatilis) is an economically and ecologically important finfish species along the atlantic seaboard of the united states. recent stock assessments in chesapeake bay (u.s.a.) indicate that non-fishing mortality in striped bass has increased since 1999, concomitant with very high (>50%) prevalence of visceral and dermal disease caused by mycobacterium spp. current fishery assessment models do not differentiate between disease and other components of non-fishing mortality ...200818839766
bioaccumulation and trophic transfer of mercury in striped bass (morone saxatilis) and tautog (tautoga onitis) from the narragansett bay (rhode island, usa).we examined the bioaccumulation and trophic transfer of mercury in two marine finfish species, striped bass (morone saxatilis) and tautog (tautoga onitis), collected from the narragansett bay (rhode island, usa). for each of these target fish, white muscle tissue was analyzed for total mercury (hg) and results were evaluated relative to fish age, body size, and hg content of preferred prey. dietary and stable isotope analysis was also used to elucidate the effect of trophic processes on hg conce ...200919150736
f-type lectin from the sea bass (dicentrarchus labrax): purification, cdna cloning, tissue expression and localization, and opsonic activity.recently described biochemical and structural aspects of fucose-binding lectins from the european eel (anguilla anguilla) and striped bass (morone saxatilis) led to the identification of a novel lectin family ("f-type" lectins) characterized by a unique sequence motif and a characteristic structural fold. the f-type fold is shared not only with other members of this lectin family, but also with apparently unrelated proteins ranging from prokaryotes to vertebrates. here we describe the purificati ...200919162197
kinetics of hepatic phase i and ii biotransformation reactions in eight finfish species.hepatic microsomes and cytosols of channel catfish (ictalurus punctatus), rainbow trout (oncorhynchus mykiss), atlantic salmon (salmo salar), red tilapia (oreochromis sp.), largemouth bass (micropterussalmoides), striped bass (morone saxatilis), hybrid striped bass (m. saxatilis x m. crysops), and bluegill (lepomis macrochuris) (n=8) were used to study the kinetics of phase i (ecod, erod, prod, brod) and phase ii (udp-glucuronosyltransferase (udpgt)-, sulfotransferase (st)- and glutathione-s-tra ...200919201019
a review of mycobacteriosis in marine fish.mycobacteriosis is a serious and often lethal disease of fish, affecting a wide range of species globally both in culture and wild settings. caused by several species of the genus mycobacterium, the disease has received considerable attention in recent years because of the discovery of new species in piscine hosts, epizootics in wild fisheries, and the ability of a few species to infect humans. the impact of this disease in aquaculture and the aquaria trade has been well reported and there is cu ...200919261041
nested polymerase chain reaction assay for detection of mycobacterium shottsii and m. pseudoshottsii in striped bass.wild striped bass morone saxatilis in chesapeake bay are experiencing a high prevalence of mycobacteriosis, which produces granulomatous lesions of the skin and visceral organs. culture-based studies have indicated that the newly described species mycobacterium shottsii and m. pseudoshottsii are the dominant isolates from diseased fish. the classical fish pathogen m. marinum is also found, albeit at much lower frequencies. both m. shottsii and m. pseudoshottsii are extremely slow-growing on stan ...200819306608
female morphology of philometra rubra (nematoda: philometridae), a parasite of the abdominal cavity of the striped sea-bass morone saxatilis (moronidae, perciformes) in the usa.the nematode philometra rubra (leidy, 1856) (philometridae) is redescribed from subgravid females found in the abdominal cavity of the fish morone saxatilis (walbaum) from south carolina, usa in november 2008. the species is characterized by the presence of 14 cephalic papillae arranged in two circles, a relatively long oesophagus with a distinct anterior inflation, and well-developed papilla-like caudal projections. cephalic papillae of the external circle differ from those in other congeners i ...200919391332
eustrongylides ignotus infecting commercial bass, morone chrysops female x morone saxatilis male, and other fish in the southeastern usa. 200919490394
historical presence (1975-1985) of mycobacteriosis in chesapeake bay striped bass morone saxatilis.a retrospective analysis of archived tissue blocks has revealed that mycobacteriosis was apparent in chesapeake bay striped bass as early as 1984. of 37 cases available from the years 1975 to 1985, 2 fish were found positive based on histopathology and genus-specific pcr. multi-gene sequencing places the bacteria from the 2 positive cases (1984 and 1985) within the mycobacterium tuberculosis clade with closest resemblance to the recently described fish pathogen m. pseudoshottsii. our data confir ...200919750805
in vitro and in vivo evaluation of the prebiotics grobiotic-a, inulin, mannanoligosaccharide, and galactooligosaccharide on the digestive microbiota and performance of hybrid striped bass (morone chrysops x morone saxatilis).two separate experiments were conducted with hybrid striped bass to evaluate four potential prebiotics: grobiotic-a (partially autolyzed brewer's yeast, dairy ingredient components, and fermentation products), mannanoligosaccharide (mos), galactooligosaccharide (gos), and inulin. in the in vitro experiment, intestinal contents were incubated with the individual prebiotics (0.5% by weight) at 25 degrees c for 24 and 48 h. analysis of volatile fatty acids in the supernatant showed that grobiotic-a ...201019844649
mycobacteria isolated from chesapeake bay fish.mycobacteriosis in fish can result in ulcers, emaciation, and in some cases death. mycobacteria have been previously isolated from a variety of chesapeake bay fish species, and the current study was designed to identify potential host specificity and location fidelity of mycobacterial isolates. mycobacteria were isolated from wild fish of the chesapeake bay collected from the upper bay, the choptank river, herring bay, the chicamacomico river, the pocomoke river and the potomac river in 2003-200 ...201019909394
expanded range and new host species of mycobacterium shottsii and m. pseudoshottsii.mycobacterium shottsii and m. pseudoshottsii are recently described mycobacteria commonly isolated from chesapeake bay striped bass morone saxatilis. however, their distribution in striped bass outside of the chesapeake region and their ability to infect alternative hosts have not been described. mycobacteria identified as m. shottsii (based on fatty acid methyl ester analysis and multigene sequencing) were isolated from striped bass collected in albemarle sound, north carolina, and white perch ...200920043404
influence of nutritional state on the progression and severity of mycobacteriosis in striped bass morone saxatilis.challenge studies with mycobacterium marinum clearly demonstrate that a poor diet affects the progression and severity of mycobacteriosis in striped bass morone saxatilis. fish (n = 512 total, wt = 65 +/- 15 g) were inoculated intraperitoneally with 10(4) colony-forming units (cfu) g(-1) body weigth (bw) or a physiological saline solution (controls) and evaluated for 8 mo. inoculated fish fed a low-ration diet (0.15% bw d(-1)) developed a severe, systemic infection characterized by a high bacter ...200920099412
quantitative pcr assay for mycobacterium pseudoshottsii and mycobacterium shottsii and application to environmental samples and fishes from the chesapeake bay.striped bass (morone saxatilis) in the chesapeake bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described mycobacterium species, mycobacterium pseudoshottsii and m. shottsii. the ecology of these mycobacteria outside the striped bass host is currently unknown. in this work, we developed quantitative real-time pcr assays for m. pseudoshottsii and m. shottsii and applied these assays to dna extracts from chesapeake bay water and sediment samples, as ...201020656856
low prevalence of splenic mycobacteriosis in migratory striped bass morone saxatilis from north carolina and chesapeake bay, u.s.a.mycobacteriosis is a chronic bacterial disease causing an ongoing epizootic in striped bass morone saxatilis in chesapeake bay, u.s.a. prevalence of disease is high in pre-migratory fish, and multiple species of mycobacterium spp. have been isolated. however, prevalence of mycobacteriosis in the coastal migratory population is unknown and is of concern to multiple coastal states, as disease-related mortality may impact the long-term health of the population. histological examinations of spleens ...201020815326
diversity in destinations, routes and timing of small adult and sub-adult striped bass morone saxatilis on their southward autumn migration.almost three-quarters of the 46 young adult and sub-adult striped bass morone saxatilis that were acoustically tagged in plum island estuary, massachusetts, u.s.a., in the summer of 2006 were detected in one or more southern coastal arrays during their autumn migration. on the basis of the trajectories along which these m. saxatilis moved from feeding to overwintering areas, three migratory groups emerged. after leaving plum island estuary, about half of the fish were detected only in a mid-lati ...201021155786
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