| comparative benzo[a]pyrene metabolite patterns in fish and rodents. | benzo[a]pyrene is converted to 3-hydroxybenzo[a]pyrene, 9-hydroxybenzo[a]pyrene, benzo[a]pyrene-4,5-dihydrodiol, benzo[a]pyrene-7,8-dihydrodiol, benzo[a]pyrene-9,10-dihydrodiol, and benzo[a]pyrene quinones by postmitochondrial supernatant or microsomes in such fish as the rainbow trout, flounder, salmon, mullet, little skate, fundulus grandis, and sea catfish. it is also now well-established that many fish convert benzo[a]pyrene to potent mutagenic metabolites as has been demonstrated with the a ... | 1984 | 6087145 |
| in vitro incorporation of [14c]glycine into muscle protein of gulf killifish (fundulus grandis) in response to insulin-like growth factor-i. | the effect of recombinant human insulin-like growth factor i (rhigf-i) on protein synthesis was studied in vitro in the muscle of a teleost fish. protein synthesis was assessed by the incorporation of [14c]glycine into protein. rhigf-i stimulated [14c]glycine incorporation in a dose-dependent manner. the minimal effective dose was 0.1 ng/ml and maximal stimulation was obtained with 1 ng/ml. a high concentration (100 ng/ml) was ineffective. treatment with 1 ng/ml rhigf-i produced a significant re ... | 1995 | 7635273 |
| immune function, hepatic cyp1a, and reproductive biomarker responses in the gulf killifish, fundulus grandis, during dietary exposures to endocrine disrupters. | the gulf killifish, fundulus grandis, was used to determine the influence of biological rhythms on three biomarker responses. we first developed monoclonal antibodies against the model's immunoglobulins and vitellogenin in order to measure antibody responses and vitellogenesis, respectively. we then treated adults with 10, 1, 1, and 10 ppm of aroclor 1254, tribuyltin, 3-methylcholanthrene, and nonyl-phenol, respectively, in mixtures over a 16-week period. the study followed vibrio anguillarum-sp ... | 2000 | 11460684 |
| field investigation on the toxicity of alaska north slope crude oil (ansc) and dispersed ansc crude to gulf killifish, eastern oyster and white shrimp. | a field investigation was conducted on a louisiana spartina alterniflora shoreline to evaluate the toxic effects of crude oil (alaska north slope crude oil, ansc) and dispersed oil (ansc + dispersant corexit 9,500) on three aquatic species indigenous to the gulf of mexico: fundulus grandis (gulf killifish), crassostrea virginica (eastern oyster), and litopenaeus setiferus (white shrimp). results indicated that total hydrocarbons concentration value in oiled treatments decreased rapidly in 3h and ... | 2006 | 16098561 |
| long term hypoxia suppresses reproductive capacity in the estuarine fish, fundulus grandis. | human nutrient input has significantly altered dissolved oxygen (do) cycles in coastal waters such that summertime hypoxia (do <2 mg/l) and anoxia of bottom water are common worldwide. prolonged hypoxia usually reduces metabolic rate in fish and potentially reduces reproduction, particularly in a spring and summer spawning species such as the gulf killifish, fundulus grandis. to evaluate the effects of long term hypoxia on reproduction, gulf killifish were subjected to either normoxia (6.68+/-2. ... | 2007 | 17596980 |
| parasitic crustaceans as vectors of viruses, with an emphasis on three penaeid viruses. | parasitic crustaceans serve as both hosts and vectors of viruses as well as of parasites and other microbial pathogenic agents. few of the presumably numerous associations are known, but many can be anticipated. recently, branchiurans and gnathiid isopods have been documented to host helminths and blood parasites. because the agents can be observed readily with a microscope, these are better recognized than are the smaller viral, bacterial, and fungal agents. some agents are harmful to the host ... | 2009 | 21669853 |
| production and characterization of microbial biosurfactants for potential use in oil-spill remediation. | two biosurfactants, surfactin and fatty acyl-glutamate, were produced from genetically-modified strains of bacillus subtilis on 2% glucose and mineral salts media in shake-flasks and bioreactors. biosurfactant synthesis ceased when the main carbohydrate source was completely depleted. surfactin titers were ∼30-fold higher than fatty acyl-glutamate in the same medium. when bacteria were grown in large aerated bioreactors, biosurfactants mostly partitioned to the foam fraction, which was recovered ... | 2014 | 24411443 |
| life cycle of calyptospora funduli (apicomplexa: calyptosporidae). | the taxonomic status of the extraintestinal piscine coccidium calyptospora funduli is based in part on its requirement of an intermediate host (the daggerblade grass shrimp palaemonetes pugio). in the present study, grass shrimp fed livers of gulf killifish (fundulus grandis) infected with sporulated oocysts of c. funduli exhibited numerous sporozoites suspended in the intestinal contents when fresh squash preparations were examined by light microscopy. using this method, sporozoites were not se ... | 2000 | 10864246 |
| biology and pathogenesis of the coccidium eimeria funduli infecting killifishes. | epizootics of eimeria funduli involved estuarine killifishes (fundulus grandis, f. pulvereus, f. similis, and f. heteroclitus) in mississippi, alabama, and virginia. all of more than 500 specimens examined of f. grandis from mississippi during 1977 though 1979 had infections, regardless of age, sex, or season collected. oocysts occurred primarily in the liver and pancreas, replacing up to 85% of both those organs. infrequent sites of infection were fatty tissue of the body cavity, ovary, intesti ... | 1980 | 7391893 |
| a new and unusual eimerian (protozoa: eimeriidae) from the liver of the gulf killifish, fundulus grandis. | oocysts and sporocysts of eimeria funduli sp. n. are described from the gulf killifish, fundulus grandis, on the basis of light microscopy, transmission and scanning electron miscroscopy, and location in the liver of infected hosts. the spherical sporulated oocysts of e. funduli isolated from liver tissue measure 20-31 (25) micrometer across with ovoid sporocysts 9-11 x 5-7 (10 x 6) micrometer. a micropyle, polar granule, and oocyst residuum are absent, but sporocysts have stieda and substieda b ... | 1979 | 522224 |
| a new species of homalometron (digenea: apocreadiidae) from fishes in the northern gulf of mexico. | homalometron palmeri n. sp. is described and reported from the following fishes in the northern gulf of mexico: micropogonias undulatus, sciaenops ocellatus, bairdiella chrysoura, pogonias cromis, fundulus grandis, fundulus similis, and eucinostomus argenteus. this species historically has been misidentified as homalometron pallidum by some, and treated as homalometron sp. by others. the new species differs from h. manteri by having a smaller body size, relatively longer postcecal space ranging ... | 2013 | 22924927 |
| characterization of isolates of streptococcus agalactiae from diseased farmed and wild marine fish from the u.s. gulf coast, latin america, and thailand. | we examined lancefield serogroup b streptococcus isolates recovered from diseased, cultured hybrid striped bass (striped bass morone saxatilis × white bass m. chrysops) and wild and cultured gulf killifish fundulus grandis from coastal waters of the u.s. gulf of mexico (gulf coast) and compared those isolates to strains from tilapias oreochromis spp. reared in mississippi, thailand, ecuador, and honduras and to the original gulf coast strain identified by plumb et al. ( 1974 ). the isolates were ... | 2015 | 26030196 |
| vertebrate osmoregulation: a student laboratory exercise using teleost fish. | here, we describe a laboratory experiment as part of an upper-level vertebrate physiology course for biology majors to investigate the physiological response of vertebrates to osmoregulatory challenges. the experiment involves measuring plasma osmolality and na+-k+-atpase activity in gill tissue of teleost fish acclimated to water of differing salinity. we describe results obtained using the widely available goldfish (carassius auratus) and a common baitfish, the gulf killifish (fundulus grandis ... | 2007 | 18057409 |
| pcr-based analysis of mitochondrial dna copy number, mitochondrial dna damage, and nuclear dna damage. | because of the role that dna damage and depletion play in human disease, it is important to develop and improve tools to assess these endpoints. this unit describes pcr-based methods to measure nuclear and mitochondrial dna damage and copy number. long amplicon quantitative polymerase chain reaction (la-qpcr) is used to detect dna damage by measuring the number of polymerase-inhibiting lesions present based on the amount of pcr amplification; real-time pcr (rt-pcr) is used to calculate genome co ... | 2016 | 26828332 |
| influence of euthanasia method on blood and gill variables in normoxic and hypoxic gulf killifish fundulus grandis. | in many experiments, euthanasia, or humane killing, of animals is necessary. some methods of euthanasia cause death through cessation of respiratory or cardiovascular systems, causing oxygen levels of blood and tissues to drop. for experiments where the goal is to measure the effects of environmental low oxygen (hypoxia), the choice of euthanasia technique, therefore, may confound the results. this study examined the effects of four euthanasia methods commonly used in fish biology (overdose of m ... | 2017 | 28397260 |
| induced pesticide tolerance results from detoxification pathway priming. | few studies in developmental toxicology have focused on whether early life contaminant exposure affects future susceptibility. investigations in frogs suggested that early life exposure to a pesticide resulted in higher tolerance to a subsequent challenge. this led to the hypothesis that early-life stage exposures can alter phenotypically plastic traits during development, resulting in induced tolerance. here, we used gulf killifish (fundulus grandis) to test the role of detoxification pathway p ... | 2017 | 28259584 |
| relative contributions of copper oxide nanoparticles and dissolved copper to cu uptake kinetics of gulf killifish (fundulus grandis) embryos. | the toxicity of soluble metal-based nanomaterials may be due to the uptake of metals in both dissolved and nanoparticulate forms, but the relative contributions of these different forms to overall metal uptake rates under environmental conditions are not quantitatively defined. here, we investigated the linkage between the dissolution rates of copper(ii) oxide (cuo) nanoparticles (nps) and their bioavailability to gulf killifish (fundulus grandis) embryos, with the aim of quantitatively delineat ... | 2017 | 28081364 |
| is exposure to macondo oil reflected in the otolith chemistry of marsh-resident fish? | genomic and physiological responses in gulf killifish (fundulus grandis) in the northern gulf of mexico have confirmed oil exposure of resident marsh fish following the macondo blowout in 2010. using these same fish, we evaluated otolith microchemistry as a method for assessing oil exposure history. laser-ablation inductively-coupled-plasma mass spectrometry was used to analyze the chemical composition of sagittal otoliths to assess whether a trace metal signature could be detected in the otolit ... | 2017 | 27682216 |
| freshwater influences on embryos, hatching and larval survival of euryhaline gulf killifish fundulus grandis and potential constraints on habitat distribution. | the influence of fresh water on potential habitat occupancy of early life-history stages of euryhaline gulf killifish fundulus grandis was determined by evaluating fertilization of freshwater-spawned eggs and subsequent survival of embryos and larvae in comparison with saline water (salinity 7). overall per cent fertilization of eggs was low (mean ± s.e. = 20·21 ± 0·03%). embryo survival was greater in saline water, but hatching rate (mean ± s.e. = 81·6 ± 0·1%) and post-hatch survival of larvae ... | 2016 | 27238386 |
| cross-resistance in gulf killifish (fundulus grandis) populations resistant to dioxin-like compounds. | the houston ship channel (hsc) in houston, texas is an aquatic environment with a long history of contamination, including polychlorinated dibenzodioxins (pcdd), polychlorinated dibenzofurans (pcdf), polychlorinated biphenyls (pcbs), polycyclic aromatic hydrocarbons (pahs), and heavy metals. populations of gulf killifish (fundulus grandis) from the hsc have adapted to resist developmental cardiac deformities caused by dioxin-like compounds (dlcs). contaminants in the hsc have acted as a strong s ... | 2016 | 27064400 |
| the potential role of polyamines in gill epithelial remodeling during extreme hypoosmotic challenges in the gulf killifish, fundulus grandis. | polyamines are a family of low molecular weight organic cations produced in part by the coordinated actions of arginase ii (arg ii) and ornithine decarboxylase (odc). although gill polyamine homeostasis is affected by acute transfer to fresh water, little is known of its function in fish osmoregulation. the current study investigated the role of polyamines in the compensatory response of hypoosmotic challenge in the euryhaline fish, fundulus grandis. adult f. grandis were acclimated to 5 ppt wat ... | 2016 | 26780219 |
| photo-enhanced toxicity of fluoranthene to gulf of mexico marine organisms at different larval ages and ultraviolet light intensities. | significant increases in toxicity have been observed as a result of polycyclic aromatic hydrocarbon (pah) absorption of ultraviolet (uv) radiation in aquatic organisms. early life stage aquatic organisms are predicted to be more susceptible to pah photo-enhanced toxicity as a result of their translucence and tendency to inhabit shallow littoral or surface waters. the objective of the present study was to evaluate the sensitivity of varying ages of larval mysid shrimp (americamysis bahia), inland ... | 2016 | 26590351 |
| experimental test of host specificity in a behaviour-modifying trematode. | host behavioural modification by parasites is a common and well-documented phenomenon. however, knowledge on the complexity and specificity of the underlying mechanisms is limited, and host specificity among manipulating parasites has rarely been experimentally verified. we tested the hypothesis that the ability to infect and manipulate host behaviour is restricted to phylogenetically closely related hosts. our model system consisted of the brain-encysting trematode euhaplorchis sp. a and six po ... | 2015 | 26394540 |
| physiological management of dietary deficiency in n-3 fatty acids by spawning gulf killifish (fundulus grandis). | lipid dynamics of spawning fish are critical to the production of viable embryos and larvae. the present study utilized manipulation of dietary fatty acid (fa) profiles to examine the ability of spawning gulf killifish (fundulus grandis) to mobilize critical lipid components from somatic reserves or synthesize long-chain polyunsaturated fas (lc-pufas) de novo from shorter-chain c18 precursors. an egg and multi-tissue evaluation of changes in fa concentrations across time after fish were switched ... | 2015 | 25939715 |
| community structure of skin microbiome of gulf killifish, fundulus grandis, is driven by seasonality and not exposure to oiled sediments in a louisiana salt marsh. | mucus of fish skin harbors complex bacterial communities that likely contribute to fish homeostasis. when the equilibrium between the host and its external bacterial symbionts is disrupted, bacterial diversity decreases while opportunistic pathogen prevalence increases, making the onset of pathogenic bacterial infection more likely. because of that relationship, documenting temporal and spatial microbial community changes may be predictive of fish health status. the 2010 deepwater horizon oil sp ... | 2015 | 25704317 |
| diet-induced fatty acid variation in critical tissues of a spawning estuarine fish and consequences for larval fitness. | freshwater and marine fishes exhibit a dichotomy in biosynthesis of long-chain polyunsaturated fatty acids (lc-pufas) from shorter-chain precursors. marine species generally lack this ability due to deficiencies in requisite desaturase or elongase enzymes. gulf killifish fundulus grandis is a euryhaline cyprinodont whose fatty acid (fa) dynamics have not been previously examined. we utilized experimental variations in exogenous fas available to spawning f. grandis in order to construct a multiti ... | 2016 | 25244374 |
| genomic and genotoxic responses to controlled weathered-oil exposures confirm and extend field studies on impacts of the deepwater horizon oil spill on native killifish. | to understand the ecotoxicological impacts of the deepwater horizon oil spill, field studies provide a context for ecological realism but laboratory-based studies offer power for connecting biological effects with specific causes. as a complement to field studies, we characterized genome-wide gene expression responses of gulf killifish (fundulus grandis) to oil-contaminated waters in controlled laboratory exposures. transcriptional responses to the highest concentrations of oiled water in the la ... | 2014 | 25208076 |
| response to comment on "multi-tissue molecular, genomic, and developmental effects of the deepwater horizon oil spill on resident gulf killifish (fundulus grandis)". | | 2014 | 24946222 |
| comment on "multitissue molecular, genomic, and developmental effects of the deepwater horizon oil spill on resident gulf killifish (fundulus grandis)". | | 2014 | 24946159 |
| evolved resistance to pcb- and pah-induced cardiac teratogenesis, and reduced cyp1a activity in gulf killifish (fundulus grandis) populations from the houston ship channel, texas. | the houston ship channel (hsc), connecting houston, texas to galveston bay and ultimately the gulf of mexico, is heavily industrialized and includes several areas that have historically been identified as containing significant levels of mercury, dioxins, furans, polychlorinated biphenyls (pcbs), and polycyclic aromatic hydrocarbons (pahs). gulf killifish, fundulus grandis, inhabit this entire estuarine system, including the most contaminated areas. f. grandis is the sister species of the well-e ... | 2014 | 24699180 |
| metabolic and embryonic responses to terrestrial incubation of fundulus grandis embryos across a temperature gradient. | this study simulated terrestrial incubation and measured rates of embryogenesis, nitrogen elimination, heart rate, lactate production, maximum length of time a hatch could be delayed and developmental responses of terrestrially incubated gulf killifish fundulus grandis embryos at temperatures ranging from 20 to 30° c. temperature had a positive relationship with rate of embryogenesis, but a negative relationship with extent of extended incubation. the 30° c treatment reached embryonic maturity 6 ... | 2014 | 24588641 |
| dispersant and salinity effects on weathering and acute toxicity of south louisiana crude oil. | chemical dispersants are an important technology in the remediation of oil spills in the aquatic environment, facilitating degradation of crude oil and salinity is an important factor in dispersant effectiveness. the aim of the present study was to explore the role of salinity on the degradation chemistry of crude oil polycyclic aromatic hydrocarbons (pahs) and acute toxicity of the water accommodated fraction (waf) of the dispersant corexit 9500a and chemically dispersed crude oil on a common e ... | 2013 | 24377102 |
| oxidative stress responses of gulf killifish exposed to hydrocarbons from the deepwater horizon oil spill: potential implications for aquatic food resources. | ecosystem effects of polycyclic aromatic hydrocarbons (pahs) remain under investigation following the gulf of mexico deepwater horizon oil spill. fundulus grandis, an established indicator of aquatic ecosystem health, was investigated because this species shares genes and biochemical pathways with higher trophic-level fish and plays an important role in the gulf food chain. oxidative stress responses including hepatic cytochrome p4501a (cyp1a) and serum antioxidant capacity were evaluated in fis ... | 2014 | 24122941 |
| multitissue molecular, genomic, and developmental effects of the deepwater horizon oil spill on resident gulf killifish (fundulus grandis). | the deepwater horizon oil rig disaster resulted in crude oil contamination along the gulf coast in sensitive estuaries. toxicity from exposure to crude oil can affect populations of fish that live or breed in oiled habitats as seen following the exxon valdez oil spill. in an ongoing study of the effects of deepwater horizon crude oil on fish, gulf killifish ( fundulus grandis ) were collected from an oiled site (grande terre, la) and two reference locations (coastal ms and al) and monitored for ... | 2013 | 23659337 |
| effects of potassium ion supplementation on survival and ion regulation in gulf killifish fundulus grandis larvae reared in ion deficient saline waters. | teleost fish often live in an environment in which osmoregulatory mechanisms are critical for survival and largely unknown in larval fish. the effects of a single important marine ion (k(+)) on survival and ion regulation of larval gulf killifish, an estuarine, euryhaline teleost, were determined. a four-week study was completed in four separate recirculating systems with newly hatched larvae. salinity in all four systems was maintained between 9.5 and 10‰. two systems were maintained using crys ... | 2013 | 23319161 |
| interindividual variation in complex i activity in fundulus heteroclitus along a steep thermocline. | the first enzyme in the oxidative phosphorylation pathway is complex i (ec 1.6.5.3). complex i is a large heteromeric enzyme complex with 45 protein subunits that translocates h(+) ions across the mitochondrial inner membrane. among northern and southern populations of the teleost fish fundulus heteroclitus, complex i subunits have fixed amino acid substitutions. additionally, there are differences in oxidative phosphorylation activity among populations of f. heteroclitus. to investigate whether ... | 2013 | 23303323 |
| rna-seq reveals complex genetic response to deepwater horizon oil release in fundulus grandis. | the release of oil resulting from the blowout of the deepwater horizon (dh) drilling platform was one of the largest in history discharging more than 189 million gallons of oil and subject to widespread application of oil dispersants. this event impacted a wide range of ecological habitats with a complex mix of pollutants whose biological impact is still not yet fully understood. to better understand the effects on a vertebrate genome, we studied gene expression in the salt marsh minnow fundulus ... | 2012 | 22971268 |
| analysis of tissue proteomes of the gulf killifish, fundulus grandis, by 2d electrophoresis and maldi-tof/tof mass spectrometry. | the gulf killifish, fundulus grandis, is a small teleost fish that inhabits marshes of the gulf of mexico and demonstrates high tolerance of environmental variation, making it an excellent subject for the study of physiological and molecular adaptations to environmental stress. in the present study, two-dimensional (2d) gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry were used to resolve and identify proteins from five tissues: skeletal ... | 2012 | 22537935 |
| embryonic development and metabolic costs in gulf killifish fundulus grandis exposed to varying environmental salinities. | the gulf killifish (fundulus grandis) is a euryhaline fish found in coastal marsh along the entire of gulf of mexico and southern atlantic of coast of the united states. the objective of this study was to investigate the effects of salinity on embryogenesis in the gulf killifish. four recirculation systems at salinities of 0.4, 7, 15, and 30 g/l were maintained at a static temperature with flow-through trays, containing embryos (n = 39) placed in triplicate into each system. throughout embryogen ... | 2012 | 22252334 |
| effects of low salinity media on growth, condition, and gill ion transporter expression in juvenile gulf killifish, fundulus grandis. | the gulf killifish, fundulus grandis, is a euryhaline teleost which has important ecological roles in the brackish-water marshes of its native range as well as commercial value as live bait for saltwater anglers. effects of osmoregulation on growth, survival, and body condition at 0.5, 5.0, 8.0 and 12.0‰ salinity were studied in f. grandis juveniles during a 12-week trial. relative expression of genes encoding the ion transport proteins na(+)/k(+)-atpase (nka), na(+)/k(+)/2cl(-) cotransporter(nk ... | 2012 | 22245490 |
| the effect of short-term hypoxic exposure on metabolic gene expression. | the long-term effect of hypoxia is to decrease both the production and use of atp and thus decrease the reliance on mitochondrial oxidative energy production. yet, recent studies include more immediate affects of hypoxia on gene expression and these data suggest the maintenance of mitochondrial function. to better understand the short-term physiological response to hypoxia, we quantified metabolic mrna expression in the heart ventricles and livers of the teleost fish fundulus grandis exposed to ... | 2012 | 22021243 |
| protein recovery and identification from the gulf killifish, fundulus grandis: comparing snap-frozen and rnalater® preserved tissues. | reliable proteomic analysis of biological tissues requires sampling approaches that preserve proteins as close to their in vivo state as possible. in the current study, the patterns of protein abundance in one-dimensional (1-d) gels were assessed for five tissues of the gulf killifish, fundulus grandis, following snap-freezing tissues in liquid nitrogen or immersion of fresh tissues in rnalater(®). in liver and heart, the protein profiles in 1-d gels were better preserved by snap-freezing, while ... | 2011 | 21898826 |
| adaptation versus allometry: population and body mass effects on hypoxic metabolism in fundulus grandis. | hypoxia has significant effects on organisms, from metabolic reduction to death, and could be an important evolutionary force affecting the variation among populations within a species. to determine intraspecific variation in hypoxic metabolism and the effect of body mass, we examine rates of oxygen consumption (m(o2)) at seven oxygen concentrations among seven populations of fundulus grandis that inhabit a mosaic of habitats with different frequencies and intensities of hypoxia. for m(o2), ther ... | 2016 | 19891563 |
| simultaneous extraction of high-quality rna and dna from small tissue samples. | purification of high-quality dna and rna from a single sample is becoming increasingly important for studies seeking both genomic and transcriptomic data. we compare different methods for isolating dna and rna from fish embryos (gulf killifish; fundulus grandis) and describe an optimal technique to extract high-quality dna and rna from a single embryo. the optimal method utilizes a chaotropic buffer and spin column technology. from embryos weighing approximately 4 mg, we were able to isolate an ... | 2011 | 18840898 |
| [some ecological aspects of larvivorous fish existing in cayo santa maría, cuba]. | one thousand and three hundred twenty one fish from cyprinodon variegatus, gambusia punticulata, fundulus grandis saguanus. cubanichthys cubensis y girardinus metallicus were collected for the purpose of locating and identifying fish of interest in the control of mosquito larvae in cayo santa maria, villa clara province. ecological indexes such as diversity (h') and equity (j') were estimated through a program named biodiversity pro v2 and also the contents in the stomach of 341 specimens (25,81 ... | 2013 | 23427465 |
| histopathological evidence of regeneration following hepatotoxic effects of the cyanotoxin microcystin-lr in the hardhead catfish and gulf killifish. | the cyanobacterial toxin microcystin-lr (mc-lr) causes liver damage in several freshwater fish species. in the present study, two estuarine species, hardhead catfish arius felis and gulf killifish fundulus grandis, were injected intraperitoneally with mc-lr at 45-300 μg/kg and their livers examined histopathologically for up to 23 d postinjection (pi) in the catfish and 5 d pi in the killifish. the livers from both species exhibited extensive, diffuse hepatocellular necrosis by 6 h pi. the necro ... | 2006 | 26599043 |
| effects of long-term hypoxia on enzymes of carbohydrate metabolism in the gulf killifish, fundulus grandis. | the goal of the current study was to generate a comprehensive, multi-tissue perspective of the effects of chronic hypoxic exposure on carbohydrate metabolism in the gulf killifish fundulus grandis. fish were held at approximately 1.3 mg l(-1) dissolved oxygen (approximately 3.6 kpa) for 4 weeks, after which maximal activities were measured for all glycolytic enzymes in four tissues (white skeletal muscle, liver, heart and brain), as well as for enzymes of glycogen metabolism (in muscle and liver ... | 2006 | 16985201 |
| oxygen consumption, blood lactate and inter-individual variation in the gulf killifish, fundulus grandis, during hypoxia and recovery. | rates of oxygen consumption (m(o(2))) for fundulus grandis, the gulf killifish, were measured in air-saturated water, at four progressively lower levels of oxygen and upon normoxic recovery. the pattern of m(o(2)) versus oxygen partial pressure (p(w)o(2)) was that of an oxygen regulator, with a critical oxygen pressure (p(c)) of 34 torr (1 torr=133.3 pa). below this value, m(o(2)) decreased and the concentration of blood lactate increased, indicating anaerobic metabolism during hypoxia. recovery ... | 2000 | 10964034 |
| apoptosis, pcna, and p53 in fundulus grandis fish liver after in vivo exposure to n-methyl-n'-nitro-n-nitrosoguanidine and 2-aminofluorene. | dysfunction in homeostatic mechanisms of cell death and proliferation are considered to be important in the pathogenesis of chemically induced neoplasia. p53 has been implicated in the regulation of cell death and proliferation. to determine whether expression of apoptosis, proliferating cell nuclear antigen (pcna), and p53 differ between an alkylating agent and a polycyclic aromatic hydrocarbon, host response was measured through sequential immunohistochemical detection of apoptosis (terminal d ... | 2017 | 10930048 |
| membrane permeability of coelenterazine analogues measured with fish eggs. | to determine the suitability of various coelenterazine analogues for the regeneration of aequorin in living cells, the membrane permeabilities of 11 analogues were measured using the eggs of the killifish fundulus grandis by soaking the eggs in solutions containing the analogues. the results indicated that e-coelenterazine, which has an exceptionally high rate of in vitro regeneration of aequorin, not only permeated poorly into the eggs but also was highly unstable. all other analogues tested pe ... | 1997 | 9291095 |
| timed daily administrations of hormones and antagonists of neuroendocrine receptors alter day-night rhythms of allograft rejection in the gulf killifish, fundulus grandis. | immune activity during scale allograft rejection, measured by melanophore destruction, is two to three times greater at night (12-hr scotophases) than during the day (12-hr photophases) in gulf killifish (fundulus grandis). in the present study of killifish, hormones and antagonists of neuroendocrine receptors were administered daily at 0800 or 2000 hr during either 12-hr photoperiods (light onset: 0800 hr) or continuous light to examine possible neuroendocrine regulation of the allograft reject ... | 1995 | 7789748 |
| nonphotic stimuli alter a day-night rhythm of allograft rejection in gulf killifish. | the influence of environmental stimuli on a daily rhythm of immune activity during scale allograft rejection was investigated in gulf killifish, fundulus grandis. although melanophore destruction in the grafts is largely restricted to the scotophases in killifish held on 12 h daily photoperiods (ld 12:12), timed daily netting (tank-transfer "stress"), thermoperiods (from 20 degrees to 30 degrees c for 4 or 12 h), and feeding altered the expression of this rhythm. melanophore breakdown peaked 0-1 ... | 2006 | 7768316 |
| a day-night rhythm of immune activity during scale allograft rejection in the gulf killifish, fundulus grandis. | the circadian variation of scale allograft rejection was studied in teleost fish maintained on 12-h daily photoperiods (ld 12:12). immune activity, measured by melanophore breakdown, was two to three times greater during the dark than during the light whether scale allografts were transplanted at light onset or light offset. because rejection occurred predominantly at night, survival times of both primary and secondary allografts were about 0.4 days longer when the transplants were made at light ... | 2006 | 8325435 |
| freerunning circasemilunar spawning rhythm of fundulus grandis and its temperature compensation. | gulf killifish, fundulus grandis, exhibit rhythmic reproductive activities that are synchronized with semilunar tidal fluctuations in their gulf coast habitat. these cyclic activities, monitored through daily egg collections, persist in the laboratory for as long as four months with periods near the tidal period of 13.7 days. in nature, a specific semilunar spawning phase is maintained with respect to tidal cycles. however, in the laboratory, the phase may gradually advance or delay. these phase ... | 1992 | 24214278 |
| combined use of biochemical indicators to assess sublethal pollution effects on the gulf killifish (fundulus grandis). | | 1988 | 3408266 |
| circadian and seasonal variations of lipogenesis in the gulf killifish, fundulus grandis. | lipogenesis was measured in male gulf killifish at three seasons under different environmental conditions. cold temperature is stimulatory for lipogenesis in the fall and spring, but warm temperature is more stimulatory during the summer. the highest rate of lipogenesis occurs during the spring. however, total body fat content is relatively low at that time, which suggests that lipids are being actively mobilized as well. a high rate of lipogenesis also occurs at a cold temperature (20 degrees c ... | 1985 | 3870846 |
| usefulness of cyprinodon variegatus and fundulus grandis in carcinogenicity testing: advantages and special problems. | cyprinodon variegatus and fundulus grandis, two species of cyprinodontid fishes studied extensively and used in toxicological and biological investigations, are compared as laboratory test animals. their ecology, general biology, and suitability for various types of experimentation are examined. a laboratory system for exposure of these species at critical life stages (embryos, fry, juveniles) to suspect carcinogens is described. a discussion of the use, findings, and potential of these species ... | 1984 | 6431292 |
| exposure to the water soluble fraction of crude oil or to naphthalenes alters breathing rates in gulf killifish, fundulus grandis. | | 1984 | 6713129 |
| effects of the polychlorinated biphenyl aroclor 1242 on locomotor activity and on the neurotransmitters dopamine and norepinephrine in the brain of the gulf killifish, fundulus grandis. | | 1980 | 6108136 |
| differences in the effects of fuel oil, an oil dispersant, and three polychlorinated biphenyls on fin regeneration in the gulf coast killifish, fundulus grandis. | | 1980 | 6775715 |
| circadian phase-dependent prolactin mechanisms in hepatic lipogenesis of a teleost. | in the teleost, fundulus grandis, injections of prolactin early in the light phase cause an immediate 50% depression in the rate of hepatic lipogenesis ([14c]acetate incorporation); 10 h later, that rate has returned to levels not different from controls. injections of prolactin late in the light phase cause an even more dramatic immediate depression of lipogenesis (79%) followed by a gradual increase in lipogenic rate which is 2.6 times higher than the control rate after 24 h. the stimulation o ... | 1979 | 512547 |
| timing of a single daily meal affects daily serum prolactin rhythm in gulf killifish, fundulus grandis. | | 1978 | 622004 |
| the anatomy and fine structure of the eye in teleost. iv. the choriocapillaris and the dual vascularization of the area centralis in fundulus grandis. | | 1976 | 1269541 |
| circadian rhythms of brain 5-hydroxytryptamine and swimming activity in the teleost, fundulus grandis. | | 1976 | 6207 |
| the anatomy and fine structure of the eye in teleost. iii. the structure of the lentiform body in fundulus grandis. | | 1975 | 1204680 |
| the anatomy and fine structure of the eye in teleost. ii. the vascular connections of the lentiform body in fundulus grandis. | | 1974 | 4140784 |
| the anatomy and fine structure of the eye of teleost. i. the choroid body in fundulus grandis. | | 1974 | 4605214 |
| daily rhythms in concentration of plasma cortisol in male and female gulf killifish, fundulus grandis. | | 1973 | 4710243 |
| daily variations in the fattening response to prolactin in fundulus grandis held on different photoperiods. | | 1971 | 5094239 |
| octospiniferoides chandleri n. gen., n. sp., a neoechinorhynchid acanthocephalan from fundulus grandis baird and girard on thetexas coast. | | 1957 | 13406675 |